LOCUS       NZ_LR135305             3220 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7429 plasmid 9.
ACCESSION   NZ_LR135305
VERSION     NZ_LR135305.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885211
            Assembly: GCF_900639465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,202
            Genes (coding)                    :: 3,086
            CDSs (with protein)               :: 3,086
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 73 of 116
            Pseudo Genes (internal stop)      :: 24 of 116
            Pseudo Genes (multiple problems)  :: 20 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135305.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7429"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            <1..534
                     /locus_tag="EQB73_RS17165"
     CDS             <1..534
                     /locus_tag="EQB73_RS17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_164857726.1"
                     /translation="ATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKK
                     FVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEE
                     FRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLT
                     SDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(671..1087)
                     /locus_tag="EQB73_RS17170"
     CDS             complement(671..1087)
                     /locus_tag="EQB73_RS17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1090..1374)
                     /locus_tag="EQB73_RS17175"
     CDS             complement(1090..1374)
                     /locus_tag="EQB73_RS17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1739..1963
                     /locus_tag="EQB73_RS17180"
     CDS             1739..1963
                     /locus_tag="EQB73_RS17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225471215.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLKYKNMTPERKRSL
                     EKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     gene            3035..>3220
                     /locus_tag="EQB73_RS17190"
     CDS             3035..>3220
                     /locus_tag="EQB73_RS17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_164857727.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMT"
                     /name="RepB family plasmid replication initiator protein"
     Typing          2..534
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctacaaaag cctttgtcgg tgatttagaa aagctctata aagatatgct taatcttact
       61 taccgtactg aagatgagga ttctattcgc tactttgttt tgtttactgg ttttgaaatc
      121 aataaaacaa aaaagtttgt tgaagtcagt gttaatccgg acctagaata tattattaat
      181 ggtttaacag ctgaatttag tagatttgaa ttatctgctt tcacgtcgtt gcgttctacg
      241 tatgcaaaaa ctctattccg actgcttatg cagtaccgtt ctactggtta ttatgtagtt
      301 cgtattgaag agtttaggag cttaatggat atcccagaat actatcaaat gggtaacatt
      361 gatcaaaaag tattaaatcc tgcattacga gaactaaaaa aatacttcaa tgatcttaaa
      421 gtcacaaaaa taaaagcaaa aaagggcaat aaaatcgcca aattagaatt tactttttca
      481 agcctaacgt cagatttgcc aaagatttct ctgcgtaact ggttagaaga ataaataaaa
      541 aagaactcgc cccccttaca ttaagcaagt agacaagtcc cttacaaccg actaagattg
      601 ctagtacatt ctagcatatt gtatggggct tctaaataaa aaacagatag gaataactcc
      661 tatctgtttt ttatcgtttt agaaaatttg ttgggtcgaa atttctagaa gtgactgttt
      721 gactggtttc tttttgatga ttctttggtc gatttcgatt tgatggattt agtttagctt
      781 cgattttttc tttcacttct tgatatactt tagaatttgt gccaataaga acgcctgtat
      841 cttttagata taaatcagca aattctgcga tgatttcagt catgatggaa atttcttttc
      901 ccatagcatt tagttttgcc tgattgctgg tagtggtcgt gttggtatcg ggttcataac
      961 tcatacataa aaagcgaatg gcttccgagt agttattgat atctggattt ttttcgatga
     1021 tggaatctaa aacgtcaaca tgactagggt acaaagtaac atgtttttgg acagaagttt
     1081 ttctcatggt tatatcattc ctccgataag catttcaatg atttgattat ttgtgtagac
     1141 atactcgctt aatatttgag tattttctgt ttggatagca ataaatttat cgagcaataa
     1201 atcgaattgg tcttttttgt aagtcgtaag ttgacggtca aaatcacttt cgattaaaac
     1261 ttttagatat ttatttcgac tccatttttc tcctgtttta ttcgtgagtt cttggcaacg
     1321 tttgtctatt gttgcaacaa actttggatc aatattccga actaaaatgt ccataaaaaa
     1381 tctccttttg caattatgta taatttatgt ataccataca aatactatat ttgtatagct
     1441 gttttttgcc aaaatactat acataagcta tccacgatgg cgaaaaagtg atatcaaatt
     1501 tttttcaaag aaacgtgata ggcacagcct atctagcacc tttcgtaaga aagtgatacc
     1561 acgtttcctt atgctcttta aaattcgggg ggagtgctgc acgtgtgatc gataaccaaa
     1621 tttaaaaatg cacgatcaaa aatttattta gtttttcaaa cgacattttt tttgagagta
     1681 tagacggcaa agaagtgatt ttatttctag aaaaattgtg agactggaga aagaaaaaat
     1741 ggaaaaattt ccttacgaat attttcaaaa cttagaaaaa ttagagcaag caattcaagc
     1801 aatcaagttc gatttaacag acgaaaaatt gttgaaatat aaaaatatga cgcccgaaag
     1861 aaaacgatcg ttggaaaaat taatgcaact agtgaacgaa gacggcggaa tttcagacgc
     1921 agcttttgac gcatggagcg aatttcatgg agaatataat taagtgggtt taaatggctc
     1981 taaaatcgct tctaagagtt ttttagcttg gaccaatgaa atacaccaaa aactaaataa
     2041 aacgtcttaa atcgaaaaat acgagctctc agagcgactt tatttgtaaa tgttgcatat
     2101 aagaaataat tacggatttt agacttttgt aggatatttt tgaaataaat atttatttct
     2161 gttataattt gattgcaaat aagtgaacac gaaacacacg aaagccccag agaggtcgga
     2221 ttctctgggg ctttttttgt gggctgagtc gccttaatta agttttactt cttgtttcgg
     2281 ttattcaacc agtaactgaa caaagcgctt aaaagcccaa caagaaggga gcaaataaag
     2341 ttagtgaaca cgaaacacac ctccttccag tcgcaagact gtcggatagg cgacaatcaa
     2401 ttatacaata aaaaagcaaa aataagccta agttttttat tgccttgggg ttgttagggc
     2461 ttgccctgac cgcctgtaag gcgctttaga acgaaggaca tagtttttat cgaatcttgt
     2521 cttagaacgg cttagacgag ctctcaggac gccacaaagg agtccctaat gaattatttt
     2581 tggcgaagga gcctaaaggc gactgctgca agtaaaatgt gagagcgtag cgatcactcc
     2641 ttcttacagc gaccataggg agctagattg gcagtttaat atcatgcgcg aacctgttta
     2701 ttttaaaaag tgggtgattt tttggcggga aattttgctc gcgcgcgcgc gtttatttta
     2761 tttatttttt aaagattttt tagaaccctt tttagggggc agttgagcct tactctccca
     2821 acggatacag aagggtacta acaacttttt atctgagtac taacaacttt ttatctgagt
     2881 actaacaact ttttatctga gtactaacaa ctttttatct gagtactaac aactttttat
     2941 ctgagtacta atatcctttt gtataataac aaccaaccta tttacgagtt ggttgttaaa
     3001 ggttattatt tatttgtaag gggggaagaa aggaatggat gaaaaaacgg aattaacagt
     3061 caaatatcaa aatgaattga atttagtacc gctgaaaaat ttcaatgcta agcagatgga
     3121 tttatttttt gcattatgcg ctcgtatgaa agatcgtggt ttgaaaaaag ttagattttc
     3181 tttcgaggaa ttaaaagagt taagtgatta taagatgact
//
