LOCUS       NZ_LR135304             5250 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7429 plasmid 8.
ACCESSION   NZ_LR135304
VERSION     NZ_LR135304.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885211
            Assembly: GCF_900639465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,202
            Genes (coding)                    :: 3,086
            CDSs (with protein)               :: 3,086
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 73 of 116
            Pseudo Genes (internal stop)      :: 24 of 116
            Pseudo Genes (multiple problems)  :: 20 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7429"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(660..1076)
                     /locus_tag="EQB73_RS17140"
     CDS             complement(660..1076)
                     /locus_tag="EQB73_RS17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1078..1359)
                     /locus_tag="EQB73_RS17145"
     CDS             complement(1078..1359)
                     /locus_tag="EQB73_RS17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353845.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIENDF
                     DHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(1735..2259)
                     /locus_tag="EQB73_RS17150"
     CDS             complement(1735..2259)
                     /locus_tag="EQB73_RS17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002305828.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KTEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(2276..3245)
                     /locus_tag="EQB73_RS17155"
                     /pseudo=""
     CDS             complement(2276..3245)
                     /locus_tag="EQB73_RS17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            4390..4968
                     /locus_tag="EQB73_RS17640"
     CDS             4390..4968
                     /locus_tag="EQB73_RS17640"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_257971661.1"
                     /translation="MKTSNRKYKVKTVNHRIPPKKALCVINNSKVINQELKPYLSEQEM
                     IKKISKDIFLTNSDTILLEKFLKKNSYFRFSIYVKLMKNIDKVTILDVIETYKLDSFIR
                     NQLHYFVNQIEIFWKKSLSDNMCVSYEETSIFPKNQCYLDKNIYSDLKWAEDIIGHFNS
                     FFYSNQSPTFKHHHIKKIIVYQYGLYLKK"
                     /name="Abi family protein"
     gene            4965..>5250
                     /locus_tag="EQB73_RS17645"
     CDS             4965..>5250
                     /locus_tag="EQB73_RS17645"
                     /inference="COORDINATES: protein motif:HMM:NF019371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_269472012.1"
                     /translation="MTFGSLTTFINQLNTEYYNNWVMSCYDNPKYKKAIRSWANVIRAY
                     RNKIAHHSRIYAFKVTDAPTIIKNDSKSYFPENNLKETQKLYLYGGLYVI"
                     /name="Abi family protein"
     Typing          complement(2281..3245)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.666"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aacatctgtt aatatatgaa gatagcatga cacaaacttt atggaataaa tttctaatag
       61 atttagaaaa aaagatcgat aaaatccctt ccttagaaaa atccaattat ggttttccgg
      121 aaaattggag aaacaagtta aaaataatga ttgtttaatt gttatttaaa ttgttagtag
      181 acataatgta catactacca agtagcccct ggatcaaccg atccagggga agactttgtg
      241 attttcaatt ggtctatcta aaacatcttt ttaaaagctt gattcttcgc tgttggcttt
      301 tttcgattct ttgtgaatat tcagtgatta tttttttact acttgttatt ttccgaggga
      361 tttgttaaaa gtttaatagc gtttctcagt aagggacgtc gttttttcat tttcctatgt
      421 atggactgct cgaacgagca gccttttttt attccaggga acgtcctatt ctttgagttt
      481 ccatgctatt gaataaccgg gacttaggga ttagggcagc gccccaaaat gaaaaaagaa
      541 aaatccgctt ctgcgaattc ttcttttttt tcgcaagttg gtaccaactt gcaaatcttg
      601 ctggaagacc gatccctaaa aagaaatcga ggaatttttt tgtttttcgg tttctttttt
      661 tatcttttta gaaagtttgt tgggtcaaaa tttccagaag taaaggtttg attggtttct
      721 ttttgacgat ttttgggttg atttcgtcgg gaaggattga gttgattttc aattttttct
      781 ttcacttctt gatatacttt agaatttgta ccgataagaa caccagtatc ttttagatat
      841 aaatcagcaa attctgccat gatttctgtc atgatagaaa tttcttttcc catggcattg
      901 agttttgctt ggttactggt tgtggttgta ttggtatcag gttcataact catacataaa
      961 aagcgaatgg cttctgaata attgttaacg tctggatttt tttcgatgat ggaatctaaa
     1021 acgtcaacgt gactgggata aagggttacg tgtttttgaa cagaagtttt tctcatgtta
     1081 tattcctccg ataagtgttt caatgagttg gttgtttgtg tgaacatagt catttagtat
     1141 gttcgtattt tctgcttgaa tggcaataaa tttttcgaat aataaatcga attgatcttt
     1201 tttgtaattc ataagttcgt ggtcaaaatc gttttcgatt aaaattttta ggtattgatt
     1261 tcgactccat tttttgcccg ttttggttgt tagttcctga caacgtttat ctattcttgt
     1321 aataattttg ggattgatat ttcgaactaa aatgtccatg gaaattttcc tttcgtatca
     1381 gtgtataatt tatgtatatc atacaaatat agtatttgta tagctatttt tcgccaaaac
     1441 actatacata gcctagccac aatggcaaaa aagtgatagc aaattttttt ctgagaaacg
     1501 tgataggcac agcttatcta gcacctttcg taagaaagtg ataccacgtt tccttatgct
     1561 ctttcaaaat tcagaaaaat cagctgtaaa acgagtgcag tccgagtaag attcaacgaa
     1621 aaaacgggga ttggggcgcc accaaaatat cgcaacatgg gcccatgttg ctctgctcgc
     1681 aaaagaaaaa agaagaataa aaccagcaat ttttattaat ttaaattgct ggttttattg
     1741 ccagaattgc caccattttt tctccgtctt tgattgtttt tctatcgatt ctgtttcttc
     1801 agaaagagtc gttgatttat tttccgaaga ttcttccgta taagtcaact gtagttgttc
     1861 ctgcagtttc tcgatttgtt ggttggcttg cagggtgagt tgttgttgtt ggtcgagtaa
     1921 tttttgcatt tgttttattt gctcgtcttt ttcttgtagt tgcatatctt tttgatttaa
     1981 ctgttgttct aggagagaaa ggacttctaa ttcgggaact tggttggcgt ttttgtggtt
     2041 ttcggtttta gttttttcgc caactagttg ttgatttgag ttttcgtttt cgttaaaaaa
     2101 cattgatttt atagcctttt gtccggtttc attaatcaca aacttgtttc cgattttcga
     2161 aaaccaaagt tttcgatttt cgtcagaaac ttttttgtta attgctgttt tactaactcc
     2221 taattcttca gcaagttctt tgattgtttt ttctgtcata aaatctaaaa acccctcaat
     2281 ctttgaataa gttttgaaga ttgtctaata agtctttttt tagttctgaa ggaatatcgg
     2341 aatttatctc tgatttttgt gcattatttt ctttattttg ggtattttgt tctctttcta
     2401 acttttctaa ctgttgtaaa agttccaatt ttcgcttttg ttctttgatt ttttcttgta
     2461 atgaagtagg tgctttttca gattgaactt gttggttagc tagttctaat gctttgtttt
     2521 gttcttcttt tgttggggca tgtttgttaa agtcgtctga tttttttatc tcaggtttaa
     2581 atgtaaattg atagccaatg acaggctttc cccgtccttt tccgtatctc ttttggattg
     2641 tcagaccttt gaagattgcg gttagttctt ggcgaatagg ctttataatt tttttattta
     2701 tatcactttc tctataactt ttagggatat caagtaattc atgaaagtct tcttttgaaa
     2761 aataggcaat tcctttggtt ctgtattgtt ttaataatct aaacatggtt ttggaataac
     2821 tgctttctaa ttcattgaat tgttggagag aatatcgaac ccattcttca agattattaa
     2881 gtaagggtaa ggctttctca tggacttgta tttcagcaaa aggaatatca gcttttccat
     2941 cgattttaaa ttgattaaat aaaacaaatc gttcacgttg caaaccgtca gcacttcttc
     3001 taccaaaacg taaatccatt aatttgtcat acgttgtttc taaatcatct atgaatcgaa
     3061 catttgcagt tgctttataa ttgcttaaat cttttaattg ttcgaatgta aattgtactt
     3121 tttttatcgc ctacgtcacg cattctagat actatagaga agaataaatt catttctact
     3181 gacgtaaatt tgcgtaaagg aatcgtattt aattcatggt ggtatttcac aatttcattt
     3241 gccataaaat cctccttcaa gattaactac ttaacaaaat tataaagagt ttctaatagg
     3301 ggtcaagccc ctaattaaag acccctaaaa cagacaaaag accctaaaaa cagacaaaaa
     3361 gacccctaaa aacagacaaa aagaccccta aaaacagaca aaaagacccc caaaagattc
     3421 tgcacacctt gggagagtaa gcatacagcc gacggtaaag aggtttaaaa agaggtttaa
     3481 aaagagatta taaaagagga gaggggcaaa atttcccgcc taaaaatttt ctgctttttt
     3541 agccaaacag gttcgcgaat tttactaaac tgggaaactt gctccctctg gtcgctaaaa
     3601 aggtgtgagg gaaaaacacc ctcacattgt acctgcgcag tcgcctaaag gctcctttgc
     3661 taaaaacgtt gtttaaagag atttttttag aggctctgcg cgctcgttta agccgtttta
     3721 agacagttta gtatggataa cccttatctt gttcttaaag caccttacag gcggtcaggg
     3781 caagccctaa caacccttaa agagcataaa accatcctaa aattcgattt aatctgtctt
     3841 atctatgttc ttgtatattt tatatacctt gggtagtaaa acgtctgaaa acgcaaataa
     3901 gagcgtatga aacaaaacaa taagcaaccg attcagcaga attccggagg agccattttc
     3961 gtacgttagc aaaaaagtgc tgttgctaaa agctgcttgc ggtgcggagc gaacaaggtc
     4021 tcctagagcg agacgtgtac aaacaagctt acaaattcgt gaaaggcacc ggttttgcgc
     4081 catttccaag caaaaaaagg tctatcgagc ttgctcgata gaccgatcgc atttagcccc
     4141 ccgcattcta acaggggggg aataatgtga aaaattatgg tagtaatgct tgatatatag
     4201 gcatttcttt tggttgcaca cttaaaaaaa atcacgattt tgacaactaa atttttaaat
     4261 aaaaaagctt tacagtttaa ttcaaatgtg ttttaatata tctatcagta cttgccacgc
     4321 ctctgattta atatcatgcg cattatggta gggctttttt tttatatttt tttaagaaag
     4381 aagattctta tgaagacatc aaatagaaaa tataaagtta aaacagttaa tcatagaatt
     4441 ccacctaaaa aggcattatg tgtaattaat aattccaaag taattaatca agagttaaaa
     4501 ccatatttat ccgagcaaga aatgataaaa aaaataagta aagatatttt tttaacaaat
     4561 tccgatacaa ttttattaga aaagttttta aagaaaaata gttattttcg atttagtatt
     4621 tatgttaaat taatgaaaaa tatagataaa gttactattc tagatgttat tgaaacgtac
     4681 aaattagatt cattcattcg taatcaattg cattattttg tgaatcaaat agaaattttt
     4741 tggaaaaaat ctctttcaga taatatgtgc gtttcttatg aagaaactag catatttccg
     4801 aagaatcagt gttatttgga taaaaatatt tattcagatt tgaaatgggc tgaagatata
     4861 attggtcatt ttaatagttt tttttattca aatcaaagtc ccacttttaa acatcatcat
     4921 ataaaaaaaa tcattgttta ccaatatggg ctttatttga agaaataaca tttggtagtt
     4981 taacaacatt tattaatcaa cttaatacag agtattataa taattgggtc atgagctgtt
     5041 atgataatcc taaatacaaa aaagctatac gaagttgggc aaacgtaatt agagcttata
     5101 gaaataagat agctcatcat tcaagaatat atgcatttaa agttactgac gctccaacaa
     5161 tcataaaaaa tgattccaaa agttattttc ctgaaaataa cttaaaagaa acccaaaagt
     5221 tatatttgta tggaggatta tacgttataa
//
