LOCUS       NZ_LR135301            15151 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7429 plasmid 5.
ACCESSION   NZ_LR135301
VERSION     NZ_LR135301.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885211
            Assembly: GCF_900639465.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15151)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:59:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,289
            CDSs (total)                      :: 3,202
            Genes (coding)                    :: 3,086
            CDSs (with protein)               :: 3,086
            Genes (RNA)                       :: 87
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 73 of 116
            Pseudo Genes (internal stop)      :: 24 of 116
            Pseudo Genes (multiple problems)  :: 20 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135301.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..15151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7429"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(<1..714)
                     /locus_tag="EQB73_RS16930"
     CDS             complement(<1..714)
                     /locus_tag="EQB73_RS16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615412.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_127841144.1"
                     /translation="MANTIIFGQQKGGVGKTTDTCMITLVAAMLMKKRTILIDIDLQAN
                     ATNFMTKTFGVETVPQTLMSALENGDLRPAIVNLKENLDLIPAGYDMRLYTEFLTENFD
                     NTIDRTFYLKNLIEEIKDDYDYIFIDIPPSTDLKVDNAMVACDYLVVVQETQQFSFEGS
                     QRLIFDYIQTLVDDFGSDVPIQIAGILPALLQQKRPMHQKLVEETIDTFGRDNVFNTII
                     QNHARLEWYPRIGFQF"
                     /name="ParA family protein"
     gene            1418..2923
                     /locus_tag="EQB73_RS16935"
     CDS             1418..2923
                     /locus_tag="EQB73_RS16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_060763466.1"
                     /translation="MKVSDVISVILKSGIREYKCLNSKIKPIGYKAEGKKGAIFGFRSK
                     KLMTESRGIVLTSQEGLAENEDKFTHWTPNVYSYGAYADEKRIIVKGHNERNLQQINTF
                     VIDFDRLTGEKLDSQMILDAAIDLELMPTLILETPGGFQAYFILENAWYISSKNNYQSI
                     EVAKRVSENLRKAFAEELPSVDLGCNHFGIARIPRTDNIVYYYPALTHDIQQLIQWSMK
                     YEPAKTYKKPLLSLVKNKYKQVNEMWYRQLISNPKIVGAKGIMGRNNVIFTLSLANYAS
                     GVDLEDCLNEMDLFNSSLERPLKDRERVRIVKSAYSGKYRGASKTYVQELVSNWGLESL
                     SEEKMFTQQRNTWYKFKKERHKRKKSHLSEWKEDILAYLETQCYRYRPEVRLKKTDLQA
                     AITFNGQSIPKRSLDRALKELMAEGKLFVQIKAGRNGGLVVATRKALIRTVIQVKQQVK
                     SAYKQGIKTFFKEADMLVRIFREPDKYSQRVDHYKQLNLWNTG"
                     /name="primase C-terminal domain-containing protein"
     gene            complement(3050..4372)
                     /locus_tag="EQB73_RS16940"
     CDS             complement(3050..4372)
                     /locus_tag="EQB73_RS16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="WP_060763465.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKLY
                     KHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKSIQ
                     EQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRPRLV
                     LTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNPILPHAKVV
                     VDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFLLDTWL
                     GNLPALKEAYELKEEFYWIWDTPDPDEGHLRYSQWRHRCMSSNSKDAYKDLVRAVDNWH
                     VEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKRKPRFNSS
                     AFNKAMLYDTFNWYEVNDHDITDNFGVDFSTPIKNLEKGDL"
                     /name="ISL3 family transposase"
     gene            complement(4532..6427)
                     /gene="merA"
                     /locus_tag="EQB73_RS16945"
     ARG             complement(4536..6427)
                     /name="merA"
                     /gene="merA"
                     /gene_name="mercury(II) reductase"
                     /tool="amrfinderplus"
                     /drug_class="MERCURY"
                     /identity="96.04"
                     /coverage="100.0"
     gene            complement(6456..6848)
                     /gene="merR"
                     /locus_tag="EQB73_RS16950"
     CDS             complement(6456..6848)
                     /gene="merR"
                     /locus_tag="EQB73_RS16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /GO_function="GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="WP_001863748.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYDD
                     VKRVGFIKRIQELGFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKRIE
                     TMLDDLKQRCPDEKQLHSCPIIETLT"
                     /name="merR"
     gene            7137..7790
                     /locus_tag="EQB73_RS16955"
     CDS             7137..7790
                     /locus_tag="EQB73_RS16955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_224813426.1"
                     /translation="MKRTWGKNLTILMLGIIITGCGEKSQETAKETKAYTFAVSTTVQS
                     LDPKLAIDPTSQLVLNNVMEGLYKLNAKGEIVPGGANGLPEISKDGLKYVIRLRKDAMW
                     SNGTPVVADDYVYSWKRTLSNQDSPNIGFFESIKNGKEIISGTMKPDKLGVYAKDKNTL
                     VINLSTPIPYFTSMLINTAFFPQKQEVVEKEGDRYGTNSDSVIYNGPYIGVHGT"
                     /name="ABC transporter substrate-binding protein"
     gene            complement(7835..8440)
                     /locus_tag="EQB73_RS16960"
     CDS             complement(7835..8440)
                     /locus_tag="EQB73_RS16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_002321606.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERPE
                     FQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNEL
                     LDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRL
                     VFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
                     /name="recombinase family protein"
     gene            8565..8852
                     /locus_tag="EQB73_RS16965"
     CDS             8565..8852
                     /locus_tag="EQB73_RS16965"
                     /inference="COORDINATES: protein motif:HMM:NF025079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="WP_060763464.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPAT
                     RLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIGSVAKF"
                     /name="DUF4158 domain-containing protein"
     gene            complement(8886..9566)
                     /locus_tag="EQB73_RS16970"
     CDS             complement(8886..9566)
                     /locus_tag="EQB73_RS16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_010710189.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPD"
                     /name="IS6-like element IS1216 family transposase"
     gene            9691..12042
                     /locus_tag="EQB73_RS16975"
     CDS             9691..12042
                     /locus_tag="EQB73_RS16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="WP_000344083.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQV
                     ELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVYTP
                     YLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLNVNY
                     LEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGNSGNY
                     VRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKGLLSSA
                     GDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTTEELNNI
                     FSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAVDLDMLGE
                     EILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMSTGYSGNLG
                     FPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNYIAKYNQKVI
                     NQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRKIYDEYRNKLT
                     NSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWLGDLMTCWGEVK
                     KLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDSTALSALKTYYCDK
                     GAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFIDKIVNNSLGTDFT
                     YLDFIGDEGKVANGFSEKIIQFQENRSKLN"
                     /name="glycoside hydrolase domain-containing protein"
     gene            12122..12517
                     /locus_tag="EQB73_RS16980"
     CDS             12122..12517
                     /locus_tag="EQB73_RS16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002304891.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIVD
                     YFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKKKE
                     YNYIVKAKVSKRFIFNPKLEIYSVVIE"
                     /name="hypothetical protein"
     gene            12842..13459
                     /locus_tag="EQB73_RS16985"
     CDS             12842..13459
                     /locus_tag="EQB73_RS16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001224538.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFKR
                     PEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTASY
                     KQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPPEFP
                     AIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
                     /name="recombinase family protein"
     gene            13500..13799
                     /locus_tag="EQB73_RS16990"
     CDS             13500..13799
                     /locus_tag="EQB73_RS16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="WP_000366408.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFLV
                     CGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
                     /name="BRCT domain-containing protein"
     gene            13833..14030
                     /locus_tag="EQB73_RS16995"
     CDS             13833..14030
                     /locus_tag="EQB73_RS16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048669782.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCPK
                     CHRRYKQKQGEYVAITIIKE"
                     /name="hypothetical protein"
     gene            14333..14665
                     /locus_tag="EQB73_RS17000"
     CDS             14333..14665
                     /locus_tag="EQB73_RS17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6440 family protein"
                     /protein_id="WP_010714745.1"
                     /translation="MPLTLILGGEYIYYDKNLGMMVAGDNSNSELIEQSDNVPLDRHKC
                     KEHVFKDKRFVEKESESLTLLGYKIVVDTVTGVNYLETPKGGITPLLDKNGKICIDKKK
                     SINKKG"
                     /name="DUF6440 family protein"
     gene            complement(14698..14982)
                     /locus_tag="EQB73_RS17005"
     CDS             complement(14698..14982)
                     /locus_tag="EQB73_RS17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010714377.1"
                     /translation="MSSLINRKIPQGATANKEADTKRKVTIQKKSSKKILASERKNIKV
                     SPSTFDLIKTISTMKSYKNYEFIDKAVESYIQNNLTEREQRILKNLISK"
                     /name="hypothetical protein"
     Typing          1419..2904
                     /name="rep_cluster_180"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.832"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaattgaaat ccaatccgtg gataccactc taaacgtgca tgattttgaa taatagtgtt
       61 aaaaacattg tctcttccaa atgtgtctat tgtttcctca actaattttt ggtgcattgg
      121 gcgtttttgt tgtaacaatg caggtaaaat gcctgcaatt tgaattggaa cgtctgagcc
      181 aaaatcgtca actaaggttt gtatataatc aaaaattagc cgttgacttc cctcaaaaga
      241 aaattgttgt gtttcttgta ctacaaccaa gtaatcacat gcaaccatag cattatctac
      301 ctttaaatcg gttgaaggtg ggatgtcaat aaaaatataa tcataatcgt ctttaatttc
      361 ttcaataagg tttttaagat aaaaagttct atctattgta ttatcaaaat tttctgttaa
      421 gaattcagtg tatagacgca tatcataacc agcgggaatt aaatctaagt tttccttaag
      481 gtttacgata gcaggacgta gatcgccatt ttcaagagca gacatcaaag tctgaggtac
      541 cgtttctacc ccaaatgttt ttgtcataaa atttgttgca tttgcttgca aatcaatatc
      601 aattaaaata gtacgctttt tcattagcat cgcagctacc aacgttatca tgcaagtgtc
      661 agttgttttt cctaccccgc ccttttgttg tccaaatata attgtattag ccatcaatat
      721 actccttcct tgtaatcaaa atgtaataaa agataaatgt atctttgtat cttttttcta
      781 tatttaaata gtaatacaaa tagaattaat tgtaaataga taaatgtatc ttttttctaa
      841 ttatcttaaa acatacttat ttaatcagat agcattttat aagaaaagtt attaaataga
      901 cttctaaata aggatacaaa gataaaaaga taaaaggata caaagataaa aagatacaac
      961 aataaaatta aaggataaaa agataaaaag ataaaaagat aaaaggatac aaagataaaa
     1021 ggatacaaaa ataaattaag aatgattatt tgagagaaaa tttttacttt acaaaagttg
     1081 tagtgacatg tattctaaag aagaatttga atgaatagaa aaaagataca tttactaatt
     1141 gaattataga caaaaaaata gagacggtaa cttagttgat ttgccgatca actaaattac
     1201 agaatcaaaa tgtacacagc atttcatgat tctctttcgt cccattatag ttttagatta
     1261 cagagagttg cagctctctt agtcttttta attatatcat ttgggacctt aaaaatcaag
     1321 attttgagct atattttagc tgcaaataag gtttaaagga gagctgtaga atgcctgcag
     1381 ttctcctttt tttaaatata gaatgggtgg tgataagatg aaagtttctg acgtgatttc
     1441 agttatttta aaaagtggta ttcgtgaata caagtgttta aacagcaaaa ttaaaccaat
     1501 cggctataaa gctgaaggaa aaaaaggtgc tatttttggg tttagatcca agaagttaat
     1561 gacggaatct cgtggaatcg ttcttacttc tcaagaaggc ttagctgaaa atgaagataa
     1621 atttactcat tggacaccta acgtttacag ctatggtgca tatgcagatg aaaaaagaat
     1681 aattgtaaaa gggcacaacg aacgcaactt gcaacaaatc aatacttttg taatcgattt
     1741 tgatcgctta acaggcgaaa aattagattc tcaaatgatt ctcgatgcag caatcgatct
     1801 ggaattaatg ccaaccctta ttttggaaac accagggggc ttccaagcat actttattct
     1861 tgaaaatgcc tggtatattt cgtccaaaaa taattatcag tcaatcgaag tggccaaacg
     1921 agtatctgag aacttaagaa aagcatttgc tgaagaattg ccttcagtcg atttaggttg
     1981 caatcatttt gggatagcac gtatccctag aactgacaac attgtctatt attatccagc
     2041 tttaacacat gatatacagc aattaatcca atggtcaatg aaatatgagc cagcgaaaac
     2101 atataaaaaa ccgctacttt cacttgttaa aaataaatat aaacaagtga acgaaatgtg
     2161 gtatcgacaa ttaattagta atccaaaaat tgtaggcgct aaagggatta tgggccggaa
     2221 taatgtgatt ttcactttat ctttggccaa ttatgcatca ggagttgact tagaagactg
     2281 tctaaatgag atggatcttt ttaacagttc tttagaacgt cctctaaaag atagagaacg
     2341 agtaagaatt gtaaaaagtg cttactctgg gaagtataga ggtgcaagta agacttatgt
     2401 acaggagtta gtgtcaaatt ggggcttaga atcgctgtct gaggaaaaaa tgtttacaca
     2461 acaaagaaat acttggtaca aatttaagaa ggaacgtcat aaaagaaaga aatctcacct
     2521 ttccgaatgg aaagaagata ttttagctta ccttgaaacc caatgttata gatacagacc
     2581 tgaagtcaga ctgaagaaaa cagatttgca agcagcgatt actttcaacg gacaatcaat
     2641 tcctaaacgt tctcttgacc gtgcattaaa agaattgatg gccgaaggta aattatttgt
     2701 acaaataaaa gctggacgga atgggggact agtagttgcc actcgtaaag cgttaattcg
     2761 tacggtcatt caagtgaagc aacaggtaaa atctgcgtac aaacagggca ttaagacatt
     2821 ttttaaagaa gccgatatgt tagtaagaat cttcagagaa ccggataaat acagtcaaag
     2881 agtagatcat tataagcaat taaatttgtg gaatacagga taaaataaaa catgtcaagt
     2941 gcctatacat tatatttctt ttatacttgt tttcgttttt gttagttatt atcctaattc
     3001 tgtatatttt tgtttttttg ggtattcgga ttttatggtg gaaaagggct tataaatcac
     3061 ccttctccaa attcttaata ggtgtggaaa aatcgacacc aaagttgtct gtaatgtcgt
     3121 gatcattcac ttcataccaa ttgaaagtat cgtataacat agctttattg aaagcacttg
     3181 aattaaatcg tggcttacgc tttttatgga gcttctcatt gaaaaggatt ttggctcgta
     3241 aggcatcaaa cgagtaacct ctacccattc gctctacctg cctaataatg ctgttaattg
     3301 actccgtata agcattagtg agccttttat caaagtagtt gaatatttcg acatgccagt
     3361 tgtctacggc tctcacgagg tctttatatg cgtctttaga gttgctggac atacaacggt
     3421 gtctccattg actataacga agatgacctt catctggatc aggagtatcc catatccagt
     3481 aaaactcttc tttgagttca taggcttctt ttaaagcagg aagattacct aaccaagtat
     3541 ctaagaggaa tgattcacgt tcatttaaat cgtgtttacg ctttagaagg ataaaccttt
     3601 cacgcataag ggtacgtctt tctttttggc tcatatgggc tttcaaagac ttcctgacgt
     3661 tatctaaggc ttgattagcc attctaacta catgaaactt atctacgaca actttagcgt
     3721 gtggaaggat agggttcact gcgtctttgt agggcttcca catatccatt gtgacgtact
     3781 caatgtaagt cctgtcactg atttctgaaa gacgttggat gactgtttcc ttgttacggt
     3841 taggcttgat gtcataaata gtcctgcgtt caatattagt caatacaagc cgaggtctac
     3901 ggataatatg tatctcgtct atcccaagcc acttaggagt ttcaaactgg tattcacgtt
     3961 ctttgagtgc cacatagtcc ttaaaaacgt tcctaatggt tttctcgtca acaccaacgc
     4021 tttctgcgac ttctacaaag gtcttagaca tggattgctc ttgaatggac tttaaaagcc
     4081 ttttggtcat actacgcttc tcatctacag atattaggcg ttcccagaag gtagatccgc
     4141 attcacgaca cttgtatcgt ctacggttca attgtaagcc cactcgcttt aaacgaatgg
     4201 gcaaatccat aattagttga tttcttgaac tgtgtttgta caacttgtca aaaccacatt
     4261 caggacaacg ttcaggtggt ccgactgctt caactttaaa catcatatcg gtttcatttt
     4321 cttgtggcgg ctctattgtt ttaatgtctg gtagggataa taagtctgac atattattca
     4381 tcctttatta ctctgcggtg ttaaggtatc aattatagat ttgatgtcga aatcatcagt
     4441 gttaaattat ttttgtgacc acaaatgcac ttagaaaatc ccttaaagca ttagctggtt
     4501 cacttgaata gggagttgta aaaaagttcc cttagcctgc acagcaagat aatttcgaaa
     4561 catctttatc aaaagttagg acagccagct tcaatccttc tgccattgtt agatatggag
     4621 ccatcgtttc tctcagatct ccaacagtta aaccgaattt cacagctaat gttgctgcat
     4681 aaattacgtc tcctgcgttt tctgccacta catgcgcccc taacactttc aatgttttcg
     4741 cgtctgccac taatttgaaa acacctgttg tttcccgatt aacgagcgct cttggaacag
     4801 catccaacgg caataccgat gttttcactt catatccttt ttcttttgcc tgttgctccg
     4861 ttaaaccaac cgttgcaatc gatggagaag taaacgtaac gcctggaacc acttctaaat
     4921 tgaccttttg atttagtcct ccgattgcat tacgagcagc aagtccacct tcataagcag
     4981 ctacataaac aaattgggga ccgagagtga catctccagc tgaataaatt cgggaattgg
     5041 tcgttttaag ataatcatca atgacaattt caccacggga accaacttca acgcctgctg
     5101 catgtaagtt taatgattct gtatttggtt ttcttccagt ggcaattagc aattgttctg
     5161 cttcaataat tcgcttttta ccatttatct caacatgaac ttttttaatg tctccatctt
     5221 gctcaactcg ttcataggtt gcacctgtta ctaaattaat tccctgttct gttaaggcct
     5281 tagtaatggc ttctgaaatt tcaggatcgt attcttttaa tagacgctcg cttctttgaa
     5341 tcaaagtgac ttctgacccg aggttatgaa atagttgtcc taattccatg ccgatatatc
     5401 ctgaaccaat tacggtaaga cgatttggaa ccttctttaa ttccaataag ctagtgcttg
     5461 ttaaataatc tacttcatct aatccgggaa tattaggtgc agttgaagaa gcacctgtag
     5521 ctattaaaaa tcttttggct gtgatttgat tgccatttac ttcaactgta ttttcattta
     5581 cgaattttgc ttcacctttt attaattcaa aaccataatc atcaattaaa ttcacatatt
     5641 tttcatttcg catctcggtt actaaatcat tcttttgttt tactaatggc gctaaatcaa
     5701 catttgaagc cgaagtgtgt aatcccacaa atggattatt ttttgctaga tgattgattt
     5761 cccctgctct taataaggtc ttagaaggaa cgcatccgac attaacgcaa gttccaccca
     5821 ccgttccacg ctcaatcata gccacttttg cgttcaaagt aacggcttca atggcagatg
     5881 aaaaggcagc tccaccagaa ccgatgatga tgtaatcata atcatagtta ccttcatcat
     5941 ttaaacttac atctgtcctt ttttccgatt gcacttgtat ttcttctgct tcgcccggtt
     6001 gatattgtgc gtcagtaatc gctgttttcg cgatatcaat gtctacatca tacggtagtt
     6061 caaacagtgc ttctccgcga cgaaagtcta cttcaatccc ttttgcacct gcattttcaa
     6121 gagcaaccgc aatatgttct tcacaaccag tgcaggtcat tccttcaacg tttagccgat
     6181 attttttcaa taaattcgtc ttttgttccg attgaaattc ttctgcttcg cccgggtgat
     6241 agtttgcgtc agcaatcgcc tttattgcac tttcaacctc aatatcatcg ggcagttcaa
     6301 atactgcttc accacgacga taactagact caatattttt agcacccatc ttttcaagtg
     6361 ctgattctac gtgtttttca caacccgtac aagtcattcc tgaaatgttt accttaaatt
     6421 tattcataaa aaagctcctt tcgttaatta atctctcatg ttaatgtttc tattattgga
     6481 cacgaatgta attgcttttc atctggacat cgttgtttta agtcgtctaa catagtttca
     6541 attcgtttta aatcctctat ttgtttttgc acttcctttt gttttttaga aacaaattcg
     6601 aacatatctt gacaacgaac ttcatcttta tctacaacac caagtaattt ataaatctcg
     6661 cttaaagaaa aaccaagttc ctgtattcgt ttaataaacc caacacgctt aacgtcatca
     6721 tatgaatata tccgataacc agcttccgtt cggtgaggtt cttgtaataa attttttcgc
     6781 tcgtaatatc tgatcgtttc tttattaact ccacatttat ctgcaaactc actaatgcga
     6841 taaatcatct tatttcaccc catgaatatt ataaaccgtg taccatagta cacgttcaag
     6901 taaattggac tattttattt ttttcccaaa gtatcactcg tatgtgattg atacttttat
     6961 ataatactat tgattatcct aacatcccac cagaaaatcc gatttaaaca ccagttgttt
     7021 ttaaactatt aagaaagtaa ttccactata tattccgaag acccgttttt tttacagtat
     7081 aattaagcta agtgttgcta gaacaatcag ataagaaatt gaataggggt gtttttttga
     7141 aaagaacttg gggaaagaat ttaacaattc ttatgttagg aataattatt actggttgtg
     7201 gggaaaagtc tcaagaaaca gcaaaagaaa ccaaagctta tacttttgct gtttctacta
     7261 cagtacaaag cttagatccg aagttagcta tcgaccctac aagccaactt gttttaaata
     7321 acgtaatgga aggattatat aaacttaatg ctaaaggtga aattgtacct ggaggagcta
     7381 atggccttcc agaaatctct aaggatggac ttaagtacgt tattcgattg agaaaggatg
     7441 caatgtggag taatggtacg cctgttgttg cagatgacta tgtttattcg tggaaaagaa
     7501 cgttaagtaa tcaagattca ccaaatattg gtttttttga atctattaaa aatggtaaag
     7561 aaataatctc aggaactatg aaaccagata agttgggagt ttatgctaag gataagaata
     7621 ctttggttat taatttgagt acgccaatcc catatttcac ttctatgcta attaatacag
     7681 cattttttcc tcaaaaacag gaagttgttg aaaaagaagg agatagatat ggtacgaact
     7741 ctgatagtgt gatttataat ggaccctata tcggggtaca tggaacatag aaacgaaata
     7801 gtgcggtaga aaagcgaatg aatgtagtat atttctacaa atcattcgct ttctttattt
     7861 tataaatagt tggtcgagag atcccgtttt cttttgcaat ctctgcaact ggaatgcctt
     7921 gctttaacat gtccaccacg gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa
     7981 tagaatactc gattggtcgt cccttatatc ggccggcttt ttgagccaat aatactcctt
     8041 gccgctgacg ttcacgaatt ttctcacgct cgttttgtgc aatataactt agcagtgata
     8101 aaaacatatc gaacatagcg gtatccagga gttcatttcc agtattaaaa tctaaaaact
     8161 gtgcatctaa tatttttaac gctacttttt tctgtttcat aaaagcaact gtatttttta
     8221 tatcttcata atctcttcct aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac
     8281 ggatataatt tagtaatttt tgaaactcag gacgctcagt atctttacca ctggcttttt
     8341 cttggaataa tttatcaatg ttaagtcctt ccatcattgc caattgtcga tccaaatttt
     8401 gatcttgtga agatactcga atatagccaa tttttcccat aaaaaactcc tttttaaaag
     8461 tgatataatt tacatgtaaa atttacttgt agttttactg ctatcatagc atttttaaaa
     8521 ataaatttta tgtagactta aaatttactt catggagggt ttaaatggat gaaatcaaaa
     8581 gtaacaaaaa agcatttggc atttatttag gagaacctag tacagaacaa ttaaaccaat
     8641 ttttttattt gcatgaaaaa gatttagaat tgatggagac tatacgacga ccagctacta
     8701 gattaggttt tgcactacag ttagtctcac tacgtttttt aggaactttt atattagatc
     8761 ttaccaatat tccccaaaat gtattgagtt atgtagcaac tcaactaaat attgatcctg
     8821 gagaaattat cggttctgtt gcaaagtttt aaataaagaa taaaatccct tacggtatct
     8881 atgatttaat ctgggattcc caataatacc ttgatttcag tacagaccga aaacccgaag
     8941 agagtgcctt cttttcgggt tttcttatat aatcctcgga tggcttccat gcctttaatc
     9001 gtgggtgagg cagtgcgtaa acttcgatag aatttattgc gtctctttac tggacgatgg
     9061 tcttgttcaa tcaaattatt caggtattta atggtacgat gttctgtccc ttgataaaat
     9121 ccgtattctt ttagtttctt aaaggcacta gcaattgatg gtgctttatc tgtgactaca
     9181 accttcggtt catcaaactg cttcactaac cgcttaagaa aagcataggc tgcttgtgtg
     9241 tcccgttttt tacgtaacca aatatccaag gttaaaccat ctgcatcgat ggctcgatac
     9301 aaataatgcc attttccttt aattttgatg tacgtttcat ccattttcca ggaataaaag
     9361 gattttttat ttttcttttt ccaaatttga tagagtagtt tgccatattc ttgcacccaa
     9421 cgataaatcg tcgtatgaga aacgttaatg ccacgatcat ataagatttc ttgaacttca
     9481 cgatagctaa ggttataacg aagatagtag cccacggcta caataatcac atcctgctga
     9541 aattgctttc ctttaaaatg attcatcgtc attcctcctg ctatcttttt ctattattct
     9601 accttatttg atagtagatt taaaactttg caacagaacc taatcaacac ataaacgaac
     9661 gtatgttcgg caagaaagca ggggattttt atggatgaga tggttttaag tactcagaaa
     9721 tggctaaaca agaaatacag taatgttaca gggtttgata aggttccaga aaatgggcga
     9781 acagggtggc ctacaatcta tggacttatt gaaggactac aagtagaact tggtattact
     9841 aatctagtag ctaattttgg acctaccact gaaaaaatgt acgataatca ggttactcct
     9901 aaatggggca aaaatctacc taaaaatatt gtatttctta ttcaaggtgc cttttggtgt
     9961 aaggggatta atccaggtgg cttcgatggg gtatatactc catatttaga tactgctgta
    10021 aaagagttgc agactgatgc agggtttaat ggaagcgctg taactggagt tttggatgcg
    10081 aaatgggcca aagcgttatt tgatatgagt gcttttgttc ttgttcccgg cggagatagt
    10141 aaaattagaa aaatgcagca atggttaaat gtaaattatt tagaatacac gggaataatg
    10201 ccttgtgatg gtatttatca aagaaattca aatcaagcat taatttttgc attacaagct
    10261 gaactaggat attctcccag tgaagcaaca gggtcttatg gtaatgggac aactagtaaa
    10321 acttatcctg tgtctgaagg taactcggga aactatgtaa gaataattca atatggatta
    10381 tatgttaatg gttattttga agacggtaca ttcgatggta ttttcactaa atatatgggt
    10441 ttagaagtac tggcattccg aaaatttatg gtattgcctg aatatactga aatagcagat
    10501 gttgttgtaa taaaaggact attgtctagc gctggagata ttagaagatc tgcagatggc
    10561 gcagatactt ctacgcaatt aactagatct caaatccaaa cattggttga taatgatata
    10621 aaaatagttg gtagatatct tactggtact gttggaattg gaaaagatga acgaaataag
    10681 tatctaacta cagaggaatt gaataatatt ttttctaaaa atttatctgt gtttcctatc
    10741 tatcaagatg gaggtgcagc attagcttat tttacttatg atcgtggctt atcagatggt
    10801 aagaaggcta tagatgcagc aaaaaatcta aatataccat taacaacagt tatttatttt
    10861 gctgttgatc tagacatgtt aggggaagaa attcttgcat atgctggaga atattttaag
    10921 ggggtatctg ctgtaatgtc atatagcggg tatcaaacgg gtgtttacgg aacaagaaat
    10981 gtttgttctc aaataatcaa taacgggtat gcttcgtttg catttgtttc agatatgtcg
    11041 actggttata gtgggaattt aggttttcct atgcctagac aatggtcttt tgatcaattt
    11101 gttgaattta ccataggaag tggcaacgga gctgttggta tcgatttaat cgctacatcg
    11161 ggtagagata gcggatttaa tgagttatca aatgatacta ataatgataa ttacattgcc
    11221 aaatacaatc agaaggttat aaatcagatg gtacgtgcgt atcaatatct atcacaagct
    11281 ggtcttgata atccttggaa ccctcatttg acattttata gatatgtaaa ttattctggt
    11341 ttgtcatggg acatcatttc ttctcctgta acagagcatg atagaaaaat ttatgatgaa
    11401 tataggaata aattaactaa tagcgagggc ctgtataatt attttataga tcctaatact
    11461 ggttcaataa taggtttgcc tcacttaatt gtgacgttac aatcacagat gtttctagcc
    11521 gacacaatca atgatagtgt ttctgacttt gcaggttggt taggagattt aatgacttgc
    11581 tggggtgaag ttaagaaatt aggaatacaa atggagcaag gtgtatttgc attagtaggt
    11641 acggctgcag gtggaacatt tagtctagaa gatttatacc aagatgttga cgcggtaaat
    11701 gtttatacct tagcaaaaag aaattctgat tcaacagctt tatcggcttt aaaaacgtac
    11761 tattgtgata agggtgctat tgatagattt aatcaatttg ttaagaacag atttggttct
    11821 tcagataaaa tcgcttcagt aacaaatagt ttattagaaa atgacgaaac agttgatatc
    11881 ttaggtgtag ggataatttc atttttcatt gataaaatag ttaataattc acttgggaca
    11941 gattttactt atcttgattt tattggagat gaggggaaag ttgcaaatgg attttctgaa
    12001 aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt tactatttaa
    12061 acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat tgtaaaagga
    12121 catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata aaaataattc
    12181 tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac ttttaataac
    12241 tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga gaaaacttcc
    12301 tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag taaaaaaatt
    12361 aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg acttcaaaga
    12421 aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat ttatttttaa
    12481 ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa attgtaaaag
    12541 ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca taaaagaaaa
    12601 actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt atgtatttta
    12661 gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt atactttttg
    12721 acatgccttg attttctttg tttatgtatg gcataataac cacataatac aatttgaaac
    12781 gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt agataaaaac
    12841 gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg aacgacaact
    12901 gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa aagccagcgg
    12961 caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa aaggagattt
    13021 actctatcta caatcattag atagactagg cagaaataaa caaatgattt tagaagaatg
    13081 gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc ctctgttaga
    13141 tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact taattctgca
    13201 attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc aaagagaagg
    13261 cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg ctttacctcc
    13321 tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag tctatgctat
    13381 ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat atgaacatac
    13441 cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag agtttgaaaa
    13501 tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg acacgaaccc
    13561 aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt acttcaaaaa
    13621 caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct ctctatacaa
    13681 aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc attttatcag
    13741 aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg tatttataat
    13801 cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat gatcgttggg
    13861 gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt gactgtgggg
    13921 aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc catcgccgat
    13981 acaaacagaa acaaggagaa tatgtagcaa ttacaataat taaagaataa acaaataaga
    14041 tttacttcaa tgttggtttc tgtttcatac taaataaaaa cctattttaa tttagtaaaa
    14101 gggaattggt ttgcaagcaa aatcttccgt tttttgatag aattaagaaa aagttgacaa
    14161 cagatcctat ttaaaaatag gatgatttgc acttgaaatt tacttattgg aataagaggc
    14221 aatctgttta atcgattgat tgtgcgtaga aaatgaacaa aaaggtaagt aacgaaagcc
    14281 gttaaaaaaa gagaaaaagg acggtaagaa aattaaagaa aggaagattt ttatgccatt
    14341 aaccttaata ctaggtggag aatatattta ttatgataaa aatttaggca tgatggttgc
    14401 cggggataat tcaaatagtg agttgataga acaatcagat aacgtaccat tagacagaca
    14461 taaatgtaag gaacatgtat ttaaagacaa acgttttgtt gaaaaagaaa gtgaatcttt
    14521 aactctatta ggctataaaa ttgtagttga tactgttact ggtgtgaatt atttagaaac
    14581 tcctaaagga ggaattacgc ctctacttga taaaaatggt aaaatctgca ttgataaaaa
    14641 gaaatcaata aataaaaaag gataaaaaga tacatgtatc tttttatcct tttttattta
    14701 ttttgagatt aaatttttga ggatacgttg ttcacgttct gttaaattat tttgtatata
    14761 gctttctaca gctttatcaa taaattcata atttttgtag cttttcattg ttgatatggt
    14821 tttaattaag tcaaaagtag agggggacac tttgatgttt tttctttctg aagctaatat
    14881 ctttttactc gatttttttt gaatagtaac ttttcgtttt gtatctgctt ctttatttgc
    14941 tgttgcccct tgtgggattt tacgatttat caaactagac atttagacta atctcctttc
    15001 ccagggaagt taattttccg tctaatatat atctcggagt ataaaaataa tcttgaatat
    15061 caccattact tttataaaga gtgatgcgtt cttctaattc acagaaaata tctgcaaaaa
    15121 gagcaatcat ttttttatca tgatagtctt g
//
