LOCUS       NZ_LR135296            10634 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7237 plasmid 4.
ACCESSION   NZ_LR135296
VERSION     NZ_LR135296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885076
            Assembly: GCF_900639455.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:13:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,940
            CDSs (total)                      :: 2,850
            Genes (coding)                    :: 2,746
            CDSs (with protein)               :: 2,746
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 36 of 104
            Pseudo Genes (incomplete)         :: 67 of 104
            Pseudo Genes (internal stop)      :: 20 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7237"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            1..933
                     /locus_tag="EQB69_RS15390"
     CDS             1..933
                     /locus_tag="EQB69_RS15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="EQB69_RS15395"
     CDS             950..1420
                     /locus_tag="EQB69_RS15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1971
                     /locus_tag="EQB69_RS15400"
     CDS             1504..1971
                     /locus_tag="EQB69_RS15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_010729430.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2098..3279
                     /locus_tag="EQB69_RS15405"
     CDS             2098..3279
                     /locus_tag="EQB69_RS15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3508..4656)
                     /gene="tnpB"
                     /locus_tag="EQB69_RS15410"
     CDS             complement(3508..4656)
                     /gene="tnpB"
                     /locus_tag="EQB69_RS15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4673..5077)
                     /gene="tnpA"
                     /locus_tag="EQB69_RS15415"
     CDS             complement(4673..5077)
                     /gene="tnpA"
                     /locus_tag="EQB69_RS15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            5706..6860
                     /locus_tag="EQB69_RS15420"
     CDS             5706..6860
                     /locus_tag="EQB69_RS15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002348708.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            6833..7276
                     /locus_tag="EQB69_RS15425"
     CDS             6833..7276
                     /locus_tag="EQB69_RS15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            7578..7958
                     /locus_tag="EQB69_RS15430"
     CDS             7578..7958
                     /locus_tag="EQB69_RS15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002348702.1"
                     /translation="MSEQNQNLASDHSKKYTYRSKPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7940..8854
                     /locus_tag="EQB69_RS15435"
     CDS             7940..8854
                     /locus_tag="EQB69_RS15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002348701.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8814..9092
                     /locus_tag="EQB69_RS15910"
     CDS             8814..9092
                     /locus_tag="EQB69_RS15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            9094..9783
                     /locus_tag="EQB69_RS15445"
     CDS             9094..9783
                     /locus_tag="EQB69_RS15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347611.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7941..8851
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatacat taataattaa tactttaatt attatcttta ataaacatag aaacttattt
     1921 cttcacagca tatttccaat tgttttatta atttttctat actttatata aaaagataga
     1981 actatttagt aagataaaaa agtagctaat tgtaattttc gtctaaaact caaaactagt
     2041 aaaaataact ttattgtgtt atttttattt ataaattatg aaaaaggacc tttcgaaatg
     2101 aaaaataata gaatctacaa aaatcagttg aattatgggc ttttattgcc agtttttctg
     2161 ctatgtatca ttggattact ttctttatat gttgctttat atcacgactc ttacactaat
     2221 tcaaaaatca caaaagaatt aattaaacaa gtattgtggt atagtattgg gttcacttca
     2281 gcaataacta tttatttttt taatcctaaa attttgtgga aattaactcc tatattttac
     2341 ggattatgca tcataatgtt attacttctc ttgaaattct ataatccaca attagcagtc
     2401 ttaactggag caaaaaattg gttttatatt gctggttttt cgtttcaacc tactgagtta
     2461 gtcaaaatag gatacaccct tatgctagct aaaataaccg tcaattatca gcaaaaatct
     2521 attactagta atacgaaaga tgattggatt tatttactta aaacttttat tgctactctt
     2581 ccaataggaa tattattatt ttttcagaac gatctaggaa caatgttagt cttcattgtt
     2641 atttatattt ttattttgtt tacgtctaac ataaatataa aaataattgc cccaataatc
     2701 gccattggtg ttgttttatc cttaatattt ttatatttgg ttatatcaga tacaggaaga
     2761 gatttcttat taaaaatagg atttcacgac tatcagttta aaagaataga ctcatggttg
     2821 aatccatttt tagatccaaa tggatctagc tatcagttag caaatagttt aattgctatt
     2881 ggctctggcg gacttttagg tactggtttt aatgtatcaa acattcacgt tcctgttaga
     2941 gaatccgata tgatattttc tgtaattgga gaaaattttg gctttatagg aagttgtctt
     3001 gtaatattcc tatactttta tttaatttat caaatgtata aaacatgttt taaaagcaat
     3061 aacttatttt tcacctatat cggtataggc attgttgcaa tgattttttt tcatgttttt
     3121 gaaaatatag gtgcaagtat cggattacta cctctaactg gtattccact cccctatatt
     3181 agtcaaggtg gttctgccat tcttagtaat tttttggggt tgggatttat gttgtgttgt
     3241 agtagatata ctaacgaaaa aaaaggaaca caaaaatgaa ttcaaaataa cactttatga
     3301 atagaatgtc tatttcaata aaaattatga taccttaaag ttaatcagtg aactactcac
     3361 cacttagcta aacctgacgg ttcttaacgc ttgaagtggg agcttcttgg gaatagagca
     3421 tacttgatag cgtatttctt tactaacagc ggtgtctctt atttaccaag ctatccccgt
     3481 agttcctacg gttcttgatg attggatcta agccaatccc atagaatgta gtcttagacc
     3541 ttcggtcaga atattgatac tagcattgat atcacgatca tgatgagtat gacaaacagg
     3601 acaagtccat tctcgaattt caagagattt cttgccatct ttatgtccgc actctgaaca
     3661 aatctgacta gacggaaacc atttatctac tttgatgatt tttcgtccat accagtcagc
     3721 cttgtattgt aatttagtca caaactttga ccaagaaaca tcagaaatac ttttagctaa
     3781 tttatgattt cgcaacatac cttttgtgtt taagtcttca atacagataa tatcgtgatt
     3841 tttgatcatt tctgtactca acttattcag aaaatcagta cgttgattca ttactttttc
     3901 atgcaatcta gctactttgc gtttttgttt ttgataattt ttagcttcaa atagattggt
     3961 acctttattt ttagccaata aagcacgcct agacaatttg cgttgttcac gctttagttt
     4021 cttttccatt ttggacgtga atttattatt atcaattttt tgtccatcag aaagaatcgc
     4081 aaagtccata atacctaagt caataccaat tgtggaatta gttttaggta attcgataat
     4141 ttcttcttta cacaacaaag aaatatagta tttaccgcta gaatgacgtg atattgtagc
     4201 ggatttgata atcccttttg gttgtctatg aagcttaatt cttactaatg acttcaattt
     4261 aggaactttg atgaatttac tatcaatcaa agcaactgta ccattttgat tattcgttgt
     4321 ataactctga acaggatttt tcttactttt gaaacgtgga aatccaacgg atttatcccg
     4381 aaaaaaattc ttgtatgctt tatctaaatt aagttgggca ttggctaaag caaggctatc
     4441 aatttctttc aaaaacggaa attctttctt gtatttagct ggtgtcggaa aagtcatttt
     4501 tttagaagaa tcatttttaa cttcttcata cgcctttttt cggtcatcaa gcattagatt
     4561 gtagacctta cggacacaac caaaagattt ggcaaagaag atttcttgtt cctctgttgg
     4621 atagattctg aatttgtatg cttttagttg ttccataaag ttcacctact ttctatttat
     4681 gattgtttcc ttgattttgg atatacttct taatcacatc aattggtgcg ccaccagttg
     4741 tcagaagaca aaaactttta gaccagaaca tttctttcca aagaaattgt ttgactcttg
     4801 gaaagtcacg ttttatcaat cttgagctgg cacttttata ggcattgatg aattttgtca
     4861 attctgtttt aggttgagct ttgaacataa tatgaacgtg gtctttatca tgattccact
     4921 ccactaaagt aatgtgatac gattctgaaa ttctttcaaa agtagttttg gcataatcag
     4981 atatttcatc atcaatcact tgtctacgat atttcgttac taaaacaagg tggtaataaa
     5041 gaagaaatac tgaatgatta tttgtatcta attccatgtt atatcaactc atttcttgta
     5101 tagactgatt gtaacatgga acaaaataaa aaggcaatac gccttagttg tcggtcttcg
     5161 agttaccaac ggtaaccctc ataccgaacg gcattcatca cccacctata gaggatgggc
     5221 gacttctgcc taattctgtt aaattcaaat tattacaaca gtcctaacga agaattgttt
     5281 gtaaaacaaa cggataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata
     5341 atgagtgttt attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata
     5401 ttttatgaaa aataacaccc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa
     5461 agaagcaact gcagaaaatc aagccttaaa aagagctttt gacctagacc aatgaaaaat
     5521 cacaaagtga gcccctgtat cccttggtag agaggctact tggcatagtc gacattatgt
     5581 taactaacgc tataaataac gattatgttc ccaaaacaat attttatgaa ctataacaaa
     5641 aatttgttat cgcatattgt atattttaat tataggtgta taccacttaa atttaacaca
     5701 aaggcgtgat tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga
     5761 tgattggaat ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca
     5821 atatcaactc ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac
     5881 actccataac ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc
     5941 ttggttcgcc atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc
     6001 cagtattttc agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa
     6061 atggagaata ttcaggaagt atttcacaat ttttagcaaa agaacttcaa aatctagtag
     6121 atagtaatat aacagataca accttatttt ttcaagataa tagcatttat tattccgata
     6181 atggacaaat taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt
     6241 cctttacaga aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct
     6301 ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact
     6361 ttattatgat taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt
     6421 acgtagaagc tgctatctta tgtaacaaaa cagatgtaca tgctacttca gctagagcca
     6481 ttatgcatta tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc
     6541 aagatcaaat aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa
     6601 tactaaacct ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa
     6661 ctgctggtta tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca
     6721 atcaaggtgg ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa
     6781 taagtgcaaa tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca
     6841 ataccattac aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct
     6901 tttcttaact agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct
     6961 aggaacttgg caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc
     7021 aaaaaatata gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat
     7081 tgataaaaat ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact
     7141 aacactgcgc tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa
     7201 gctcgtattt attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt
     7261 ttttaaagct gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt
     7321 gaaacaagcg aaactttaca aaaagtggct agcatttgct agcctttttt gtattgtgga
     7381 gcgtgggttt tgagggggat tcaaggggga gcttgcgccc ctttgaaaag cggttatttg
     7441 caccaaatac gtaggtattt ggctagcaaa tatagagctt gccaagccct tttggtgagc
     7501 tttactcatt gtgccttttt aggtacaatg gtattgcgaa cgatacgtcc gtaggacgta
     7561 ctgaaagggt gggacttatg agcgaacaaa atcaaaattt ggctagcgat cattctaaaa
     7621 aatataccta tcgatccaag ccaaaacaaa tcagttttcg agtgagcgaa tctgaatttg
     7681 caaagctgaa gcagtcagct gaagctttgc aaatgagtgt gccggctttc gtgaaagcca
     7741 aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgcc caggcaatcg
     7801 cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg tgcaatacac
     7861 accaacatga cgttgctcct ggagacgccc aacgtttatc cgaaaaccta aaaatcatgc
     7921 aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt cctgtagccg
     7981 agcaatcaat tacgcagaac cacgagcaac cattaaaaca ggaataaact gtgacgtcga
     8041 ttatgccaaa agtgaaatga aacaaattcg catgctgtat ggaaaagacg accgtgtaca
     8101 ggcacactta ttgattcaat cttttcgacc aggagaaatt accgctgaaa aagctaatca
     8161 actaggggca gagtatgcaa aaaaaatagc ccccgatcac caaacagcta tttacacaca
     8221 tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc tggagactgg
     8281 aaaaaaattt caagctcatg gtcaagcatt tctaaacaaa tgttatgacc taaatgacga
     8341 gatttgtctt gcacataact taccaatcac tgagcgagga aagaaaccag aaaaacgaac
     8401 catgtctgaa ataaaattaa aagaaaaaaa tgaacctgtt tggaaagacg aaattcggat
     8461 tgcgatcgat caaacaatga aaaacaaaaa aaccagaacc tatgaccaat tttgtaattc
     8521 cctcaaaaca ttggggattc attgcttcaa tcgcgggaaa aatttcactt acgaattgat
     8581 ccacaaaaag aaaaaagtac gatccaacag actaggaaag gactacgaaa aggagacgat
     8641 tttacatgag ctggacagac gagaacgaac ctataaccaa cagcgaacca acgagctacg
     8701 aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac aacgaactct
     8761 tacgcctgaa caaccggaaa ttagaccggc tcgaagaaac catgaacaac acgttgaaag
     8821 aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacata gaaaacgaaa
     8881 taaagaaaca acgtgctttt ctcgttcctt atacgcaaga aaggatacaa actacgattg
     8941 atttacaagt taaaaaactc catcaagagc tattagacca gcgaaataag gaacagaaaa
     9001 aggtgcttat tcaacgatca ttggattttc ttgccccctt atgttgctta gtcctgacac
     9061 tgttcgcatt ttggttagta tggggaggct aaaatggcat tttatttacc ctatctactc
     9121 atttttgtta gtatttcagg aagtatttgg ctcatctata aaatttttca aacaagatat
     9181 tcattaaaag gctctaaaat tcgatttaag cgatttttct ttcttggctg tatattttcc
     9241 ttgataatcg tttcaagcgg cttattcggc gttttagagg gaaataagcg cgtttctaga
     9301 agtattttat taggaaatgt cactcaaaag tatgaatcag cgcgtgataa aaagaaaaaa
     9361 gagcaagcat tagcacaaaa aatggaagag tttaccactt gttatgagga aatgaacgat
     9421 atttttgtga atcaagaaaa acgtttgacg gataaaaaca tggaaaaact cactcgtctt
     9481 tatcaaaact tgccagaaaa acagcaaaaa gaggttcaag acaactatga gcgaaccaaa
     9541 aaagacgtgc aatatgtgaa agatacgaaa gtggaagaag cttgtagtga tttatttgga
     9601 gatacaattc ctttttcaac tagcgaacaa gaacgaaaag aacgacaaca atcagtcaca
     9661 tatgaacgct atgaaatctt gcttcaacaa gcaattaaca tacaaaatct gactaaaaaa
     9721 gaaacagctt taaattattt aaaaagcgtc aaggaatggt tagatcagca acagcaaaat
     9781 tgaatttgat tgaaattcat gttaaactaa acatgcaaat gttcaaacat tagatacacg
     9841 aaagccccag agaggtcgga ttctctgggg ctttttttag gttgatgtcg cctttactga
     9901 ttttacttct tgtttcggtt attcaaccaa taactgaata cagagataca aactcctaca
     9961 agaagggggc aaataaacag ttcaaacatc agatacacct ccttccagtc gcaagactgt
    10021 cggataggcg acaagttatt ataccctaca aaattaaatc tgtgtggcaa ttttaagtat
    10081 ctcttatttc cgggttgtta gggcttgccc tgaccgcctg tagggcgctt taaattccag
    10141 ataaagtttt ttatactaag actgtcttaa aacggcttag acaagctctt agagcctcaa
    10201 ccctcctttt tgaacaatgg ttttagcaaa ggagctggag gcgactgcgc aggtataatg
    10261 tgagagcgta gcgatcacac ctttttggcg accgtaggga gcgaggtttt gaattagcag
    10321 tttagaaaag ttcgcgaacc tgtttaaaga aaaaataatt gtttttttag gtgggaaatt
    10381 ttgtctccct cttttataat ctttttttaa acctcttttt aaacctcttt aacgtcggct
    10441 gtgtccttac tcccccaagg tatacaaaat tttataggca tgaaattatc tgtttatagg
    10501 catgaaatta tctgtttata ggcatgaaat tatctgttta taggcatgaa attatctgtt
    10561 ttaggtattg tttttagaca taatatgtcc taaaatgatg tcaataaaat catttagact
    10621 ggagtaacaa ctaa
//
