LOCUS       NZ_LR135291             9319 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7199 plasmid 5.
ACCESSION   NZ_LR135291
VERSION     NZ_LR135291.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885040
            Assembly: GCF_900639445.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9319)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:08:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,110
            CDSs (total)                      :: 3,018
            Genes (coding)                    :: 2,897
            CDSs (with protein)               :: 2,897
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 43 of 121
            Pseudo Genes (incomplete)         :: 77 of 121
            Pseudo Genes (internal stop)      :: 21 of 121
            Pseudo Genes (multiple problems)  :: 17 of 121
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135291.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7199"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(<1..225)
                     /locus_tag="EQB72_RS16670"
     CDS             complement(<1..225)
                     /locus_tag="EQB72_RS16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127843690.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAP"
                     /name="hypothetical protein"
     gene            complement(185..1099)
                     /locus_tag="EQB72_RS16255"
     CDS             complement(185..1099)
                     /locus_tag="EQB72_RS16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1081..1461)
                     /gene="mobC"
                     /locus_tag="EQB72_RS16260"
     CDS             complement(1081..1461)
                     /gene="mobC"
                     /locus_tag="EQB72_RS16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(1903..2040)
                     /locus_tag="EQB72_RS16500"
     CDS             complement(1903..2040)
                     /locus_tag="EQB72_RS16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(2154..2774)
                     /locus_tag="EQB72_RS16265"
     CDS             complement(2154..2774)
                     /locus_tag="EQB72_RS16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(2771..4897)
                     /locus_tag="EQB72_RS16270"
     CDS             complement(2771..4897)
                     /locus_tag="EQB72_RS16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(4951..5325)
                     /locus_tag="EQB72_RS16275"
     CDS             complement(4951..5325)
                     /locus_tag="EQB72_RS16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            5458..5919
                     /locus_tag="EQB72_RS16280"
     CDS             5458..5919
                     /locus_tag="EQB72_RS16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(6232..6402)
                     /locus_tag="EQB72_RS16505"
     CDS             complement(6232..6402)
                     /locus_tag="EQB72_RS16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(6418..6957)
                     /locus_tag="EQB72_RS16285"
     CDS             complement(6418..6957)
                     /locus_tag="EQB72_RS16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(6985..7722)
                     /locus_tag="EQB72_RS16290"
     CDS             complement(6985..7722)
                     /locus_tag="EQB72_RS16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            8368..8454
                     /locus_tag="EQB72_RS16295"
     CDS             8368..8454
                     /locus_tag="EQB72_RS16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(8575..9264)
                     /locus_tag="EQB72_RS16300"
     CDS             complement(8575..9264)
                     /locus_tag="EQB72_RS16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..9319
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6986..7722)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(189..1099)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggggcaaga aaatccaatg atcgttgaat aagcaccttt ttctgttcct tatttcgctg
       61 gtctaatagc tcttgatgga gttttttaac ttgtaaatca atcgtagttt gtatcctttc
      121 ttgcgtataa ggaacgagaa aagcacgttg tttctttatt tcgttttcta tgtcttttaa
      181 cgttttagag cgataattct tgttggtggt ctgttcctct ttcaacgtgt tgttcatggt
      241 ttcttcgagc cggtctaatt tccggttgtt caggcgtaag agttcgttgt tctcttcgta
      301 cttcttctgc agtttcgcga atgctagctc gagctcttcg tagctcgttg gttcgctgtt
      361 ggttataggt tcgttctcgt ctgtccagct catgtaaaat cgtctccttt tcgtagtcct
      421 ttcctagtct gttggatcgt acttttttct ttttgtggat caattcgtaa gtgaaatttt
      481 tcccgcgatt gaagcaatga atccccaatg ttttgaggga attacaaaat tggtcatagg
      541 ttctggtttt tttgtttttc attgtttgat cgatcgcaat ccgaatttcg tctttccaaa
      601 caggttcatt tttttctttt aattttattt cagacatggt tcgtttttct ggtttctttc
      661 ctcgctcagt gattggtaag ttatgtgcaa gacaaatctc gtcatttagg tcataacatt
      721 tgtttagaaa tgcttgacca tgagcttgaa atttttttcc agtctccaga ttaacggagt
      781 taatgacaat gtgattatga atatggtctt tgtctgtatg tgtgtaaata gctgtttggt
      841 gatcgggggc tatttttttt gcatactctg cccctagttg attagctttt tcagcggtaa
      901 tttctcctgg tcgaaaagat tgaatcaata agtgtgcctg tacacggtcg tcttttccat
      961 acagcatgcg aatttgtttc atttcacttt tggcataatc gacgtcacag tttattcctg
     1021 ttttaatggt tgctcgtggt tctgcgtaat taattgcacg actacaagaa gtggttcggc
     1081 ttaccttaat tgttgccata tcttttgtag ctccttttgc atgattttta ggttttcgga
     1141 taaacgttga gcgtcaccag aagcaacgtc atgttggtgt gtattgcacc acttagcaat
     1201 ttgattgata ttccctccag ctttggctaa atcgcgcgcg attgcctggg caatgtctgg
     1261 cgccactttc ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga aagccggcac
     1321 actcatttgc aaagcttcag ccgattgctt cagctttgca aattcggatt cgctcactcg
     1381 aaaactgatt tgttttggct cggatcgata ggtatatttt ttagaagaat tgctagccaa
     1441 attttgattt tgttcgctca taagtcccac cctttcagta cgtcctacgg acgtatcgtt
     1501 cgcaatacca ttgtacctaa aaaggtacaa tgagtaaagc tcactaaaag ggtttggcaa
     1561 gctctatatt tgctagccaa atacctacgt atttagtgca aataaccgct tgtcaaaggg
     1621 gcgcaagctc ccccttgaat ccccctcaaa agccacgctc cacaatacaa aaaaagctag
     1681 caaatgctag ccactttttg taaagtttcg cttatttcac cgaagatgaa attgttactt
     1741 tgcaacaaaa aacataaatg cttttttgtg taaactacta actatttagc taaaactgat
     1801 agttttatta aacattcagc aatattttta gccaattttt tatcagactt tcggcgagtc
     1861 attatgataa tatgtccact ttttgttact tctaccaaaa atctagcaat aactctccca
     1921 ttcttcagtt gatttagttt cataagtctt ttgtaattga cggtttaatt tttttggcaa
     1981 ttgcaaagaa taaataagtc tatcataaaa tttttcatac tcttttttca ataaaatcac
     2041 tagggtgcct cctttttatt aagtgattat ttaaattttg ggataggaga gatggaatat
     2101 aaaataaata cccgtggtgc tagttttaaa tcagctagca tcacgggtaa aaattataat
     2161 tctataatat tagttccata gtttagaaca tttaagtcat gtgttactag tacaacggct
     2221 tttttttctt tttctgttaa atttttcaga aagttcatta taaaagttga gttttcttta
     2281 tctaaatttc ctgtaggttc atctgcaaat aatatatcaa atggttttaa catatttcta
     2341 attaatgcta ttctttgttg ttctccaccg cttaagagaa agattttttc ttttaagata
     2401 tcttttggca aagaaaaatc tgttaataaa ttataaatat gatcattgga aatattactg
     2461 ttaaactttt ttgctaattc gaaattgtat tgtactgttt gattttcaag aagagcatag
     2521 ttttgaaata aatagctaat atgatttctt cgtagacgtt gtatatcatg tttagaaact
     2581 ttcttttcaa aatagaataa gttaccggta tattgtttat ctattaggct taaattattt
     2641 aacagagtag tttttccact accacttttt cctgttacta taataaaatc tttttctgat
     2701 attgaaaagt tccaattttg tttgatcttt tttttattaa ttgacagggt gaaattctca
     2761 actgataata tcataaaata ttctccttaa aatatttaaa actatttttt ttataaataa
     2821 taatagaagt aattagattt attaaaaaga tcataaaaat tataaaatac aagtaagtgt
     2881 tttttgaaaa tatcaattga aaacacatag tagcaaataa ctctattgta aaagggagta
     2941 aacataaatc taaaacttga cttattggtg aatatccaaa tatttttttt aaagctattt
     3001 tttttgaatt tatagtaaaa ctaacaaaaa tcatttgaaa attaattatt ataaataatc
     3061 ctaatattaa aagatattgt agatatgcat ttaatttatt tacttttttt tgagataata
     3121 attcaccagt ttcttgaaaa acagaataag aaaaatttat gatattttct aggttaaatt
     3181 cttttatata cggattaatt tgattaaaaa agttttggtt tgaagtatgt ttctgatcaa
     3241 aaaataatcc accatcacta gtcaatatat ttccccaaaa taatgattct gttatttctg
     3301 gattaataac tctaactatg ggatcagtta ttgtatagtt atcttttgcc tttccatata
     3361 aggtattaaa actaaaatac ttttggttat tattaacaaa tattagtttt atttttaaat
     3421 ttttcaaatt aggattttta taaggagctt gaggaggaga gtcctcaagt ctaaactgta
     3481 tgtcttctaa ataatttttt tcaatcattt ttgcatattc tttatatttt ttaggtacta
     3541 gaatgtaagt agttagtgga tcagagttta ttaaatttaa gtcagtagat tcttcaaaag
     3601 aaataggatt gtaatatttt agatagttta aatcaacaca tattcctgca ccagcaggtt
     3661 gactaacata gtcttcaaaa tttggataaa ctttttgtcc aataaaaaat tctttattat
     3721 tctcttggat agttgcgtaa ttgtatggcg cgataataaa agttttataa ttcttctgta
     3781 ttttataaac aaatttacta gcttttttca agtagttatt ttcttctacg gtgttgtttc
     3841 tatttaattg attagtaata tttgttttgt ataaattagt agttctttcc caatatttaa
     3901 gagaattatt ttcggcttca ttatgggtat ttattacttt tgtagaagct attaacagaa
     3961 ggatcatgct taagaataga cagatattta atatacaaag atttaactta agtggtcgtg
     4021 aattttttag ataatttttt atactagata aatgatagat acttagagta actatgataa
     4081 ataaaaaact aattataaaa tgaaaaatac tacatagaag aaatgatagg aaaaaatttt
     4141 cgttactgta atatatgctc aaaaaaatag ataaaatgaa agttataatc aaagaacaaa
     4201 ataagaggtt taactcttta atagaatctt ttatagtatc aaaagagtta tagcctaaca
     4261 ttttttttag agtgatttct ttcatttttg aaaaagcaaa tataattgta gtaattacaa
     4321 taaatatact aagatagcag ataacaagaa caatattagt attaatttga aaagtatctg
     4381 attgattgat attggaaata gtaggtgttc catatttttt cagtacagga attaatttgt
     4441 ttgagtttcc ttgtacatat aatatttcac ttaatccaat attttccaca tgagaaaaag
     4501 gataaatatt tatatttaaa aaattatttg gaagttttaa ttggtactta gaatttttgt
     4561 tattagtata atggacttta gaatcgtttc ttgctaatat atttttttta gaaaatttcc
     4621 agtgtttgtt agcgttaggg tttgtagcaa gaatagttag ttggttaaca gcagtaaaac
     4681 tatattgaga aatattaata ttttcttgtg tagatatttc atacaaagcg tttatataac
     4741 tgttaaagtc catatcatct ttatattgca tatgaatttc agcggtattt ttatatagcg
     4801 cttcagaagt ctttatacca tttataacat tagtagaaaa aaatagtatt gtaatgcaga
     4861 caaaaagtga taaaacagcg tttatttttt ttaacatatt ttgaatttcc tttcaaatgt
     4921 aaataaaaaa gagagtttaa actctctttt ttaataaaca ttccaccaac cagtatttcc
     4981 agtaagtgtg gcattgcatg ttgcataagc tcttactcca gctttctgaa ctgatcgtac
     5041 ttgaccgcct cgtccatttt tagctgttgc actatgttta gctctagcat ggtagtaatt
     5101 tgcataaact ttaaatccat taaccccaca gttgaaatcc ccgccgccag cagcatatct
     5161 caatttacta gaagaaaatc cttcttctgt agcttccatt cctcctccgt aaatggcggt
     5221 atggtcttcg ggtacatcta gatttaaagc ttctgtggca ctagctattc ctccactagt
     5281 taataagaca cccattaaag cagaaagaca taaaaatttt ttcataactt atttctccta
     5341 tcttttttac tagaattatt ttattttagt gtgttttata aaataaagaa cttttttggc
     5401 gcgttagtat ttttatgata aaattttatg taaataatcg tttacatagg agaaaaaatg
     5461 gaggatttag gatctaagct aagagaatta agagtgaaaa aagggtatac tcaagaatat
     5521 gtggcaagaa aatttaacgt tacacgtcaa actatttcaa agtgggaaaa taacaaaaca
     5581 acaccagata tagagacact aaaaaaaatt tgtgaatttt atagtactaa cataaattct
     5641 attttaaatg aagataattc aaaaaataaa aaaaataatt tactggtatt gacagctatt
     5701 atttttaata tatttgttac tatattaggt ttatttgaat tgattcaaat gaattttttt
     5761 agctttacac atggaatatt tacttggttt gctctatctg gattaatagt aatagatatc
     5821 ttaaattatt tttatattta ctattcttat ttaaaaaatg aaaaattttt ttatacaatg
     5881 tttttccccc tagttatttt gattatttta ttaagctaat atgaagtagt tagttattta
     5941 attgcatagt tgacataatg tcgactatgc caagtagcct ctctaccaag ggatacaggg
     6001 gctcactttg tgatttttca ttggtctagg tcaaaagctc tttttaaggc ttgattttct
     6061 gcagttgctt ctttccgttt ctttgtgaat ttttaccagt cattttttac tggggtgtta
     6121 tttttcataa aatatgataa atttttggta gcgcttcata agcgcgttgc tttttcaaga
     6181 ataaacactc attatttttt aactggctgc tcggaagagc agtctttttt attatttatc
     6241 cgtctctttt acaaacaatt cttcgttagg actgttgtaa taatttggat tcaatttcaa
     6301 ttcaccatgt ttattccctt tattgattac tgacaataac atcggactga agcctagaat
     6361 tcctagtaaa atagaaccga atagaagatt ttttcttgtc atgaatgttc cctccagtta
     6421 tttccaaagt tgccaccatt tttgcttctt cttttttgtg tgtttttcta tcgattctgt
     6481 ttcttcagaa agaatagtag ttgattcatc ttctggagtt tcttccgtat aagctaattg
     6541 taactgttct tgcagttttt cgatctgttg atttgcctgt agggtgagct gttgttgctg
     6601 gtcgagtaat ttttgcatat tagaattatt ttgctctttg agggaaagtt gttctcgtaa
     6661 aaattcattt tcttcacgca acttatctag caactcgttt acaatcacgt ttttgcttga
     6721 tatataaacg tttttatcgt tttcttgttc atactcatgc aacctatata gtaacctttg
     6781 gttacctaag ttgctagcaa gttgcaatac tccagaacgt aatacaacct tataaccacg
     6841 ctcattttta gctaataagg acgggtctaa tttatcaaca tgtttagaga ttgcttgcct
     6901 acttatttca aataattcag ataattcctt tattgtaaag gtttctttgg gttgcatgat
     6961 aattgcctcc taaattgtta ggtattattt ctctaaccaa ttatctaagg taattgaagg
     7021 tatattttct tcaaaataaa tttgcagaga tcctatctta tttcctttgt aggctttaat
     7081 ttttttgatt tcaaaagttt taaaaatagc agggtgagtt gaattaacgt ttaaaaattc
     7141 ttttttcgct tgggcaaaaa tttgtttatc aatatctctc atgcgataac tttctggtat
     7201 tgctaataga gctctaaaat cttctatttt tacaaaccag tgaccttttt tagatcttct
     7261 gtctttatga gccataagtt ggcgatataa ctctttaacg tagctactct ttaagtttgt
     7321 catattttct aattcgaaac gagtaaaatt agttccgatt gaattaagta tatattcaaa
     7381 ttcttcagaa acctttatta ataatgtttc attcacttta tcgattttaa atttttgaaa
     7441 cagatttaat tgttcatatg ttttttcgtc ttcataaata aaagacaaag taatgatttt
     7501 ttttgacatt tttattaaat cattagcaaa acggtcgcca cgtgcattta tattgtaatc
     7561 aattaattct tttatttgtt taaatgtaaa aatttcttct tttgtacctt ttcttttcat
     7621 tttagaacat attccccaaa ataaatccat atctataggg gtaaattttc ttaaagggac
     7681 agaatttaat ccattattat atcgaactat atcgttactc aataaaaatc acctcaataa
     7741 attatgttat catagacata agaggatgta aaacacaatt tatcccccat agtgtgacaa
     7801 tttatccccc atagtgtgac aatttatccc ccatagtgtg acaatttatc ccccatagtg
     7861 tgacaattta tcccccacta cctatgtcaa tacttactct cccataaggt tgctaatccc
     7921 taacagttat tattaacagt atatctaaca gtatatctaa cagtatatct taagagcaca
     7981 ttgaatttcc cgcctaaaaa actcctactt tttcctccaa aaaggttcgc gaatttttct
     8041 aaactggaaa acttgctccc tacggtcgct aaaaaggtgt gatcgctatg ctctcatatt
     8101 atactgcgca gtcgcctccg gctcctttgc taaaaccgtt gttcaaagag gaggtgtgag
     8161 actctgaaag cttgtctaag ccgttctaag acaattcttg attaaatttc ccttaatcta
     8221 gtcgagaagc gccttacagg cggtcagggc aagccctaac aaccctaaaa caagtgatac
     8281 ttaaaaacac cacacaaatt taatttttta agatataata acttgtcgcc tatccgacag
     8341 tcttgcgact ggaaggaggt gtatctaatg tttgaactgt tcatttgccc ccttcttgta
     8401 ggagtttgtg tctctttatt cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa
     8461 taaaggcgac atcaacctga aaaaaagccc cagagaatcc gacctctctg gggctttcgt
     8521 gtatctaatg tttgatcatt tgcacattca gtttaacatg attttaaatc aaattcaatt
     8581 ttgttgttgc tgatctaacc attccttgac actttttaga tagtttaagg cggtttcttt
     8641 ttttgtcgga ttttgcatgt tagttgcttg ttgaagtaag atttcatagc gttcatatgt
     8701 gacagattgt tgtttttctt ttttttcttc ttcactggca aaccaagggt ttgtgtctcc
     8761 aaatagatca ctacaggctt cttctatctt tgtatctttt acatattgca cgtctttttt
     8821 ggttcgctca tagttgtctt gaacctcttt ttgctgtttt tctggcaagt tttgataaag
     8881 acgagtgagt ttttccatgt ttttatccgt caaacgtttt tcttgattca caaaaatatc
     8941 gttcatttcc tcataacaag tggtaaactc ttccattttt tgtgctaatg cttgctcttt
     9001 tttcttttta tcacgcgctg attcatactt ttgagtgaca tttcctaata aaatacttct
     9061 agaaacgcgc ttatttccct ctaaaacgcc gaataagccg cttgaaacga ttatcaagga
     9121 aaatatacag ccaagaaaga aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata
     9181 tcttgtttga aaaattttat agatgagcca aatacttcct gaaatactaa caaaaatgag
     9241 tagatagggt aaataaaatg ccattttagc ctccccatac taaccaaaat gcgaacagtg
     9301 tcaggactaa gcaacataa
//
