LOCUS       NZ_LR135285             4305 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6975 plasmid 8.
ACCESSION   NZ_LR135285
VERSION     NZ_LR135285.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884860
            Assembly: GCF_900639395.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,323
            CDSs (total)                      :: 3,230
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 84 of 139
            Pseudo Genes (internal stop)      :: 31 of 139
            Pseudo Genes (multiple problems)  :: 25 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135285.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6975"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(<1..333)
                     /locus_tag="EQB65_RS17345"
     CDS             complement(<1..333)
                     /locus_tag="EQB65_RS17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_164857622.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGSY
                     LEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIFMT
                     LTTPNVK"
                     /name="protein rep"
     gene            complement(922..2328)
                     /locus_tag="EQB65_RS17350"
     CDS             complement(922..2328)
                     /locus_tag="EQB65_RS17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_002304901.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDENI
                     YLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIGTL
                     DDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDEVSP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREENEREK
                     ALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(2550..2912)
                     /locus_tag="EQB65_RS17355"
     CDS             complement(2550..2912)
                     /locus_tag="EQB65_RS17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002313294.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRKSQ
                     MSESGFASMFSDVNSR"
                     /name="hypothetical protein"
     gene            complement(2916..3224)
                     /locus_tag="EQB65_RS17360"
     CDS             complement(2916..3224)
                     /locus_tag="EQB65_RS17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002304909.1"
                     /translation="MDKIDLREETVNRIEVMYERLADYLNLSDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQISVINSKEKGM"
                     /name="hypothetical protein"
     gene            complement(3233..3652)
                     /locus_tag="EQB65_RS17365"
     CDS             complement(3233..3652)
                     /locus_tag="EQB65_RS17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305964.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGIIL
                     LDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            complement(3649..>4305)
                     /locus_tag="EQB65_RS17370"
     CDS             complement(3649..>4305)
                     /locus_tag="EQB65_RS17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_127840800.1"
                     /translation="AECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRND
                     YNPHFHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKG
                     FQQEKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLD
                     RYKKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
                     /name="protein rep"
     Typing          complement(926..2328)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.359"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttacattc ggtgtcgtca acgtcataaa aataaattga tactgctttt cttccgcaat
       61 agcttgcatc ataatcgaca acatcatcga atccttacga gctttacgcc aagcacataa
      121 cggacagaaa cgatttttac aaaagtgagc ttggaccaat ttctttttct ccttatcaat
      181 cgttgcaata aattctaaat atgaaccaca gcctgtcatc aattcacgca ttttgggaga
      241 aattcgatta tcactaaatg ccaccacttt tttcaaattt ttcttttttt ctcgaaatgt
      301 tccgttaatc aaatcttgct ttttcttttt catcttgcta tactgaatct acaaattttg
      361 tatacaaaaa aggctgaaaa gccgataaca aaaaatagat tgctctcctt tctagtttag
      421 tttttttcta gtcaattaaa actataaaat actagggaag aaaaagcaat cttttttttt
      481 gttcttcctt agagccacaa gggatagacc catactatag ccccgatttc ttcgcagaaa
      541 tagttgccta tagtatcaag ataagaaaaa acatcaaaaa agagggcgaa aatcgccccc
      601 tttttttcgt tttaaaaccc caaaacccaa agacataaaa ttcgatcaat cgaccactgc
      661 atttttaagg gggtgtacat attttgtaca tttaaccgcc tgttagatag ggctgttaaa
      721 ttttcccact cattcttagt catgcaaaca taaaaccgca ccctttaaaa agggcgcccc
      781 caaacttcgc taacgctaag ttggccaaaa aatggacttg ccatttttta gcaaatgtca
      841 gcttaatagg ctagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt
      901 accgttgact gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttatg
      961 ctgtgagacg ttttgacgat aaaataaggt tttctcctgc tctaatactt ctaattcctc
     1021 agaaagcccc atatcctttt ctgcaagccc aaaacggata gacaaacgag tgatcgattt
     1081 ttgaaatgcg ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag
     1141 ctttttattt tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc
     1201 ttccaatgca gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg
     1261 ctcactcatg ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa
     1321 atcaacaaaa tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc
     1381 taaaggaacc atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt
     1441 tttcttctct tcttcaatct tcgctctctc agaagccacc agagccttct cacgctcgtt
     1501 ttcttctctt tgcctacaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac
     1561 ggccgtttga cgcaattcgg aaatttcgct catgatttct ttgtattcat gaatatcttt
     1621 gattttgtta gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat
     1681 tgactcaact tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc
     1741 tcgtaaaccc tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt
     1801 atacccttca gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg
     1861 aataacagca ttgtagacgg ataaactagg attccgttct ttaaattctt tgaaatactc
     1921 aactaaaatt ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt
     1981 agaagaacca tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa
     2041 ttctcgctgt ggttccattt ttttatcctt gcgaaccttt gctaaatagt cctcaatttt
     2101 tctatcggct cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc
     2161 atatttttca cgaatattta tttgttccaa atagatattt tcatccgatt ttgtgaaatc
     2221 aatatgctta tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga
     2281 aatgcttgta ttctttgttc cctttttgaa cgaaatacta aaccccaagt taaacccctc
     2341 cttcgcgctg tcgctaataa aaagagtgta aacctttctg ttactctttg tcaaagagta
     2401 acgttttcct aattgtgaca tgactgtcac aattccaaac gctctccgag ggttttcgcc
     2461 gtcacaagtg acaacgaaaa aaggagccga aagcgtgtca cgctctcaac tccttttacg
     2521 ctttattttg cttcatgcaa aatattcgct tatcgtgaat ttacgtcact aaacattgac
     2581 gcaaatccac tctcactcat ctgacttttt ctattcggta tccctccgaa tattccgtca
     2641 gattcgttag ggtaattccg ttcgcttggt gctgattttt catcagctcg caactcatta
     2701 cccactgatt tttcgtgttg ctgatcgtca gaaatcgacg atagccaccg ctcaaaatca
     2761 gcaaaagatt caaacccaaa acgcttgcga agagccttgc ccattgcagc attttctttc
     2821 tcaatcaatc gtttcttctg ttggcgaagt ttcgcctgtt ctttttttaa tttttcttct
     2881 tgcactttaa tatctgctaa agtcattccc ataaactaca tccccttttc tttggaatta
     2941 ataacggaaa tttggtttaa ctgcataata tgactcatta atttcaacca aaattctttt
     3001 tcagcttcat cagttgtttg cgctaaatgc ttatgaacaa attcattagt caaaactgaa
     3061 gattttgcta gatcagcatt gataaccgag ttgagaacat ccatcattcg ttcaagcatc
     3121 tcactataat tatcttcatc cgaaagattc aaataatcag ctaaccgttc atacatcact
     3181 tcaattctat tgactgtttc ttctctcaaa tcaattttat ccatgtcaca tctcacttcc
     3241 agaattgcca ccattttcga ttttttaatt cttgattttc tagtaacaac tgatcgtttt
     3301 tttgttgctg aagcagggtc aatttttgtt gatcacgaat catttctgtt aattcagaaa
     3361 tatgtttgtc cttctcagct aattgatcac gcaaaaactc attctctttt tccaaaggaa
     3421 aatcactgcc cgtgctattg accggtaaat gaccagtcaa ttgaccacct tttgaccgat
     3481 aaattgaccg agcaacacca atcaactctt tgtctagcaa gataattcca tttttcttgt
     3541 accgagcaag atcatatccg gctttgacta gataatttct cactgtattt ttggttattc
     3601 ctagttcatc agcaagcgcc ttaattgtca ttttatccgt tgaatcagtc attcattttc
     3661 cccctcaaca gatttttcat cgacatacat tcctgaaaaa taatttcttt catcagcact
     3721 catctcacga taatcaaccg catatttcga ttctttcctc atccacgaag cagtcaaaaa
     3781 ccaaaaatat tcattctcat cttttttctt ataacgatca aggtcacccg actcatattt
     3841 tttcttgtat tctttgaata caccgttaaa cgtaatcaac tgacgacctt tcattgcatg
     3901 atagaatgtg tcaaacactt cctgactttc ggtcagttca aaatcttttg ccgaatattt
     3961 cgcaacttcc tgtactgctt tttcttgttg gaaccctttg accttgcgaa tatccaactg
     4021 ggtaatttcg tcggtaccat ccggattaat tcccgttttt ccggtcactt cccgccacaa
     4081 atctaaccat tccgattgct tgatataata atctttatcc gtgaaataag atttatttac
     4141 tgccaaaatc aagtgaaaat gcgggttata gtcattccgt tttttgttgt acgtgatttc
     4201 caattttcgc acatagcctt taatcgaagt cttaacccgt ttgcgcataa acatcttttt
     4261 caatgcatga ttaaacaaat caatttcatc gttcaaacat tccgc
//
