LOCUS       NZ_LR135283            12090 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6975 plasmid 6.
ACCESSION   NZ_LR135283
VERSION     NZ_LR135283.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884860
            Assembly: GCF_900639395.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12090)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,323
            CDSs (total)                      :: 3,230
            Genes (coding)                    :: 3,091
            CDSs (with protein)               :: 3,091
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 54 of 139
            Pseudo Genes (incomplete)         :: 84 of 139
            Pseudo Genes (internal stop)      :: 31 of 139
            Pseudo Genes (multiple problems)  :: 25 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135283.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6975"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            1..945
                     /locus_tag="EQB65_RS17200"
     CDS             1..945
                     /locus_tag="EQB65_RS17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..>1067
                     /locus_tag="EQB65_RS17205"
                     /pseudo=""
     CDS             966..>1067
                     /locus_tag="EQB65_RS17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /name="HTH domain-containing protein"
     gene            1142..1822
                     /locus_tag="EQB65_RS17210"
     CDS             1142..1822
                     /locus_tag="EQB65_RS17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            <1877..2053
                     /locus_tag="EQB65_RS17960"
                     /pseudo=""
     CDS             <1877..2053
                     /locus_tag="EQB65_RS17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /name="GNAT family N-acetyltransferase"
     gene            2146..2940
                     /gene="aph(3')-IIIa"
                     /locus_tag="EQB65_RS17220"
     ARG             2147..2937
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <3350..3433
                     /locus_tag="EQB65_RS17965"
                     /pseudo=""
     CDS             <3350..3433
                     /locus_tag="EQB65_RS17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
                     /name="MLS leader peptide"
     gene            3482..3565
                     /locus_tag="EQB65_RS17230"
     CDS             3482..3565
                     /locus_tag="EQB65_RS17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="WP_031929417.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuator leader peptide
                     ErmL"
     gene            3690..4427
                     /gene="erm(B)"
                     /locus_tag="EQB65_RS17235"
     ARG             3691..4424
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     gene            complement(4690..5553)
                     /locus_tag="EQB65_RS17240"
     CDS             complement(4690..5553)
                     /locus_tag="EQB65_RS17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="WP_001284311.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKT
                     PKLPGI"
                     /name="zeta toxin family protein"
     gene            complement(5555..5827)
                     /locus_tag="EQB65_RS17245"
     CDS             complement(5555..5827)
                     /locus_tag="EQB65_RS17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="WP_000301765.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVN
                     LLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
                     /name="antitoxin"
     gene            complement(5844..6059)
                     /locus_tag="EQB65_RS17250"
     CDS             complement(5844..6059)
                     /locus_tag="EQB65_RS17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="WP_001835296.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETA
                     KNGGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            complement(6218..6898)
                     /locus_tag="EQB65_RS17255"
     CDS             complement(6218..6898)
                     /locus_tag="EQB65_RS17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            6925..7416
                     /locus_tag="EQB65_RS17260"
     CDS             6925..7416
                     /locus_tag="EQB65_RS17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_232043243.1"
                     /translation="MILPYLIVDLKLCNRTDSSMIAKNERGYKVVLRSGVLQLARNLDN
                     QRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQLALKEQNNSHIQKLL
                     DQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMDEQTKNWKKWWQLWK
                     "
                     /name="DUF536 domain-containing protein"
     gene            7431..7601
                     /locus_tag="EQB65_RS17575"
     CDS             7431..7601
                     /locus_tag="EQB65_RS17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032491833.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            8056..8280
                     /locus_tag="EQB65_RS17265"
     CDS             8056..8280
                     /locus_tag="EQB65_RS17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="WP_002363584.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="class II bacteriocin"
     gene            8300..8587
                     /locus_tag="EQB65_RS17270"
     CDS             8300..8587
                     /locus_tag="EQB65_RS17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_002363589.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="bacteriocin immunity protein"
     gene            9037..9417
                     /locus_tag="EQB65_RS17280"
     CDS             9037..9417
                     /locus_tag="EQB65_RS17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_010782660.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9399..10313
                     /locus_tag="EQB65_RS17285"
     CDS             9399..10313
                     /locus_tag="EQB65_RS17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010782659.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKKKVRS
                     NKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTTEQPEI
                     RPTRRNHDQHVERGADYQQELSF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10558..11247
                     /locus_tag="EQB65_RS17295"
     CDS             10558..11247
                     /locus_tag="EQB65_RS17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002369882.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     ARG             3691..4427
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     BGC             3056..12090
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9400..10310
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.421"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgggt tctgttgcaa
     1081 agttttaaat ctactatcaa ataaggtaga ataatagaaa aagatagcag gaggaatgac
     1141 gatgaatcat tttaaaggaa agcaatttca gcaggatgtg attattgtag ccgtgggcta
     1201 ctatcttcgt tataacctta gctatcgtga agttcaagaa atcttatatg atcgtggcat
     1261 taacgtttct catacgacga tttatcgttg ggtgcaagaa tatggcaaac tactctatca
     1321 aatttggaaa aagaaaaata aaaaatcctt ttattcatgg aaaatggatg aaacgtacat
     1381 caaaattaaa ggaaaatggc attatttgta tcgagccatc gatgcagatg gtttaacctt
     1441 ggatatttgg ttacgtaaaa aacgggacac acaagcagcc tatgcttttc ttaagcggtt
     1501 agtgaagcag tttgatgaac cgaaggttgt agtcacagat aaagccccct ctattacaag
     1561 tgcctttaag aaactaaaag aatacggctt ttatcaaggg acagaacatc gtaccattaa
     1621 atacctgaat aatttgattg aacaagacca tcgtccagta aagagacgca ataaattcta
     1681 tcgaagttta cgcactgcct ctaccacgat taaaggcatg gaagccattc gaggattata
     1741 taagaaaacc cgaaaagaag gcactctctt cgggttttcg gtctgtactg aaatcaaggt
     1801 attattggga atcccagctt aaatcataga taccgtaagg gattttattc tttatttaaa
     1861 actttgcaac agaaccgaat gggcaaagca taaaaacttg catggactaa tgcttgaaac
     1921 ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc
     1981 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
     2041 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
     2101 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
     2161 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
     2221 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
     2281 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
     2341 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
     2401 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
     2461 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
     2521 gacatatcgg attgtcccta tacgaatagc ttagacagcc gattagccga attggattac
     2581 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
     2641 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
     2701 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
     2761 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
     2821 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
     2881 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
     2941 tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat ctaatctttt
     3001 ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta aaagttcgct
     3061 agataggggt cccgagcgcc tacgaggaat ttgtatcgat aagaaataga tttaaaaatt
     3121 tcgctgttat tttgtacatt taacttgacg gtgacatctc tctattgtga gttattagtg
     3181 gtacagtttt caaccgtttt aattataaaa aagtggtgca tttttaaatt ggcacaaaca
     3241 ggtaacggtt attgcaggtg tatttcttat ctatgggttt aacatggatt ttatcattaa
     3301 aatcatgagt attgtccgag agtgattggt cttgcgtatg gttaacccta aagttatgga
     3361 aataagactt agaagcaaac ttaagagtgt gttgatagtg cattatctta aaattttgta
     3421 taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt
     3481 catgttggta ttccaaatgc gttatgtaga taaaacatct actgttttga aacagactaa
     3541 aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt gactaatctt
     3601 atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct gtatttgttt
     3661 atagatgtaa tcacttcagg agtgataaca tgaacaaaaa tataaaatat tctcaaaact
     3721 ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta aaagaaaccg
     3781 ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa
     3841 gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat
     3901 taaaactgaa tactcgtgtc actttaattc accaagatat tctacagttt caattcccta
     3961 acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta
     4021 aaaaagtggt ttttgaaagc cgtgcgtctg acatctatct gattgttgaa gaaggattct
     4081 acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga
     4141 ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg
     4201 tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg aagctatata
     4261 cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt
     4321 ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag
     4381 tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc
     4441 ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata
     4501 gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa aaaacagcga
     4561 aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg accccaacat
     4621 gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac tcttttcctt
     4681 ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg taggtggctg
     4741 caagctttcc aatttttgtt gaattgcttt aaattcaggt gtctcttggt gtttatttag
     4801 aaccattttt tgctctattc gttctaaagt cggttgaatt tctttccctg atactttacg
     4861 attcaattct ttttctaagg tttcttttgg actaatagaa ggtgtttcca aacttgaata
     4921 gagttttact ccttctctgt tatatagcct tatatcgcta aataagcccg ttttatgaag
     4981 ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt ttggtgttgc
     5041 cctggctgtc attggatcat ctgcatacat ggtttcatat cgttcaattg ttcctaaata
     5101 tgagttgatt ttaggtactg ccatgacata catttttgtt tcataacctt tggcttgaag
     5161 cattgttgcg gtttgaatag gaacgtctgt tgttcgtcct gtaccttcga tcaccaaatt
     5221 atacccttga tcgctcaaac ggcttatgat cgcttctgtc atgcgattag aataaggggt
     5281 aacgtgtttt actacgtctt tttcataaag tttcactagt tcatcaaaat tagggtgctg
     5341 ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt caaaaattgc
     5401 tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag cggttggcga
     5461 ttcaaccgct ttttttcctt gaatcaattc ttctaaatta tcatttaagc gattctcaaa
     5521 ttgtttgtca gtaaaattga ctatatttgc catattaagc cactttctct ttattcaaaa
     5581 cttgttttga gtaacgattc attgaccgcc aatactcatg aacggcttgt aattcttcta
     5641 aagaataatc aaaaagattt acacgttttg caagctttaa ttggtttaat aaattgactt
     5701 ccaataattg agagtcattt ttatttaatt catggttcaa aacatagttt aatactcgat
     5761 tataaacgct tgccgataat tcgttaatga tctctatttc aaatgttttt tcatacgtaa
     5821 ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg tatatattct
     5881 tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt ttcgattttt
     5941 atgatatggt gcaagtcagc acgaacacga accgtcttat ctcccattat atcttttttt
     6001 gcactgattg gtgtatcatt tcgttttgct ttttgtgcgc ctaaatttcc cacaatcact
     6061 cacttctttc tatttcttct tactcttatt ttatcatcaa caatcacaaa tcacaagtga
     6121 tttgtgatta atcacttgtg atttgtgatt ctattctttt tcgggttctg ttgcaaagtt
     6181 ttaaataaag aataaaatcc cttacggtat ctatgattta agctgggatt cccaataata
     6241 ccttgatttc agtacagacc gaaaacccga agagagtgcc ttcttttcgg gttttcttat
     6301 ataatcctcg aatggcttcc atgcctttaa tcgtggtaga ggcagtgcgt aaacttcgat
     6361 agaatttatt gcgtctcttt actggacgat ggtcttgttc aatcaaatta ttcaggtatt
     6421 taatggtacg atgttctgtc ccttgataaa agccgtattc ttttagtttc ttaaaggcac
     6481 ttgtaataga gggggcttta tctgtgacta caaccttcgg ttcatcaaac tgcttcacta
     6541 accgcttaag aaaagcatag gctgcttgtg tgtcccgttt tttacgtaac caaatatcca
     6601 aggttaaacc atctgcatcg atggctcgat acaaataatg ccattttcct ttaattttga
     6661 tgtacgtttc atccattttc catgaataaa aggatttttt atttttcttt ttccaaattt
     6721 gatagagtag tttgccatat tcttgcaccc aacgataaat cgtcgtatga gaaacgttaa
     6781 tgccacgatc atataagatt tcttgaactt cacgatagct aaggttataa cgaagatagt
     6841 agcccacggc tacaataatc acatcctgct gaaattgctt tcctttaaaa tgattcatcg
     6901 tcattcctcc tgctatcttt ttctattatt ctaccttatt tgatagtaga tttaaaactt
     6961 tgcaacagaa ccgattcttc catgatagct aaaaatgagc gtggttataa ggttgtattg
     7021 cgttctggag tattgcaact tgctcgcaac ttagacaatc aaaggttgct agataagttg
     7081 cataaacatg aacaaaacaa cgataaaaat gcttatatac caaacaaaaa cgtggttgca
     7141 aacaagttgc tagatcagtt gcgtgaagaa aatgaatttt tacgagaaca acttgccctc
     7201 aaagagcaaa ataattctca tatacaaaaa ctactcgacc agcaacaaca actcaccctg
     7261 caagccaacc aacaaatcga aaaacttcag gagcaactgc agttgactta tacggaagag
     7321 tcttcagaga atagttcaac gactctttct gaagcaacag aaacaatgga cgaacaaaca
     7381 aagaattgga aaaaatggtg gcagctatgg aaatgaagaa ggaaatactt atgacaaaaa
     7441 aacacttgct attcggttct atcctactag gaatattaag ctttagccca atgttattat
     7501 cagtggccaa caaaggaaat aaaaaagggg aactaaaact caatcctaat tactacaaca
     7561 gtcaaaatga ggaagtattt gtagaaaaac ttgataaata agaaaagact gctcgtacga
     7621 gcagtctttc tgaaagagat tatcctatta ttaaccttag aaaagaataa taaatgaata
     7681 tgagatatgt aaaaagcata acaaacacaa acaaaaataa caagtattta aaaaatgacc
     7741 gatcaatatt cacaaagaat tagaaagaaa caaccgcaga aaaccaagcc ttaaaaagag
     7801 cttttaacct agaccaataa aaattcacaa aatcagcgcc tggatccctt ggtacaggcg
     7861 ttacttggta gtatacacat tatgtctact agctgtttaa ataactgatc ttataatttt
     7921 atttaaacga ttatgaattc aaaactactt tttatgacgt taatcattgt tatataataa
     7981 aatgttatat aataaaatta ctaagattat ttaatataac aagcaatttt aaaaaaacga
     8041 aaaggaaaaa caatcatgaa aaagaaagta ttaaaacatt gtgttattct aggaatatta
     8101 ggaacttgtc tagctggcat cggtacagga ataaaagttg atgcagctac ttactatgga
     8161 aatggtcttt attgtaacaa agaaaaatgt tgggtagatt ggaatcaagc taaaggagaa
     8221 attggaaaaa ttattgttaa tggttgggtt aatcatggtc catgggcacc tagaaggtag
     8281 tggtagggag gttattataa tggattttac taaagaagaa aaacttttaa atgcaattag
     8341 taaagtatac aatgaagcaa ctatagatga ctatcctgac ttaaaagaaa agctctttct
     8401 ttattctaaa gaaatcagtg agggaaaaag tgttggtgaa gttagtatga aattaagtag
     8461 ttttcttgga agatatattt taaaacataa atttggatta cctaaatctt taatagaatt
     8521 acaagaaatt gttagtaagg aatctcaagt atatagagga tgggcttcta ttggtatttg
     8581 gagttaattc tttggaataa aatgaaaatg ggactgaaga atcaattctg attcatcagt
     8641 cccataaatt atagagccga aaaattatca tatttataga tttattaata gtaaatggca
     8701 taagaatctt tgaacaacga aaaaaatgta gctctaccta attattgatt tgcaacaatt
     8761 tcaccgaagg tgaaacaagc gaaactttac aaaaagtggc tagcatttgc tagtcttttt
     8821 tgtattgtgg agagtggggg ggagggggat tcaaggggga gcttgcgccc ctttgacaag
     8881 cggttatttg caccaaatac gtaggtattt ggctagcaaa tatagagctt gccaagccct
     8941 ttagtgagct ttagtgagct ttacttattg taccttttta ggtacaatgg tattgcgaac
     9001 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa tcaatatttg
     9061 gctagcaatc attctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     9121 gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg
     9181 ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     9241 gacattgctc aggcaatcgc gcgcgattta gccaaagctg gcgggaatat caatcaaatt
     9301 gccaagtggt gcaacacaca ccaacacgac gttgatcctg gcgacgccca acgcttatcc
     9361 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagcc
     9421 gaaccacttc ttgtagccga gcgatcaatt acgccgaacc acgagcaacc gtcaaaacag
     9481 ggataaactg tgacattgat tatgccaaaa gcgaaatgaa acaaattcgc atgctgtatg
     9541 gaaaagacga tcgtgtacaa gcacacttat tgatccagtc ttttcgacca ggagaaatta
     9601 ccgctgaaaa agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc cctgaccacc
     9661 aaatcgcgat ttacacacat acagacaaag accatattca taaccacatt gtcattaact
     9721 ccgtcaatct ggaaactggg aacaaatttc aagcccatgg tcaagcattt ttaaacaaat
     9781 gttatgacct aaatgacgag atttgtcttg aacatgactt aacaatcact gaacgaggga
     9841 agaaacccga aaaacgaacc atgtctgaaa tcaaattaaa agaaaaaaat gaacctgttt
     9901 ggaaagacga aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa actcgaacgt
     9961 ataaccaatt ttgtgattct cttaaaacat tcggcattca ttgcttgaat cgtgggaaaa
    10021 attttactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaaa ctaggaaagg
    10081 attacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgagcc tacaatcaac
    10141 aacgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca aaagaagtac
    10201 gaagagaaca acgaactctt acgactgaac aaccggaaat tagaccgact cgaagaaacc
    10261 atgaccaaca cgttgaaaga ggagcagact accaacaaga attatcgttc taaaacgtta
    10321 caagacatag aaaatgaaat aaagaaacaa cgtgcttttc ttgcacctta taccaaagaa
    10381 agaatacaaa ctacaattga ttcacaagtc aaaaaattcc accatgcgct attaaaccag
    10441 cgaaataaag aacagaaaaa agtaattatg cagcgatcaa tggatattct tgccccctta
    10501 tgttgcttaa tcatgacact gttagccttt tggctggtat ggggagaata aatagaaatg
    10561 gcattctatt taccctatct actcattttt gccagtattt taggaattat ttggcttagc
    10621 tataaggctt atcaaacaag atattcatta aaaggctcta aaattcgatt taagaggttt
    10681 ttattgccag gggttatatt tacattatca atcgttttaa gtggtttatt cggcgtttta
    10741 gagggaaata aacgtatttc aagaagtatt ttattagggg atatcactca aaagtatgaa
    10801 gcagcatgtg ataaaaagaa aaaggagcaa gtgttaacac aaaaaatgga agagtttacc
    10861 acttgttatg aggaaatgaa cgatattttt gtgaatcaag aaaaacgttt gacggataaa
    10921 aacatggaaa aactcactcg tctttatcaa aacttgccag aaaaacagca aaaagaggtt
    10981 caagacaact atgagcgaac caaaaaagac gtgcaatatt ttaaagatac gcagacagaa
    11041 gaagcttgtt ataatttatt cagtgataca atcccttttt caactagtga acaggaacga
    11101 aaagaacggc aacaatcagt tacctacgaa cgttataaag cactgcttca gcaagcaacg
    11161 aacatacaaa atccaacgaa aaaagaaacg gcattaaatt atctaaaaag tgtcaaggaa
    11221 tggttagatc agcaacagca aaattgaatt tgatcggcaa tcatgttaaa ctaaacatgc
    11281 aaatgatcaa acattagata cacgaaagcc ccagagaggt cggattctct ggggcttttt
    11341 ttcaggttga tgtcgccttt attgatttta cttcttgttt cggttattca accaataact
    11401 gaataaagag acacaaactt ctataagaag ggggcaaatg aacaattcaa acattagata
    11461 cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatatc ttaaaaaatt
    11521 aaacttgtgt ggtgttttta agtatcgctt attttagggt tgttagggct tgccctgacc
    11581 gcctgtaagg cgctttaaag ctagataagg atttttatcc aagactgtct taaaacggct
    11641 tagataagca ttcagaaccg ctcaaaggcc tctttaccaa catgttttta gcaaaggagc
    11701 ctttaggcga ctgcgcagta caatgtgaga gcgtagcgat cacacctttt tggcgaccgt
    11761 aggaagcaag gttttggatt ggcagttttg aaaaatgcgc gaacctgttt aaagaaaaaa
    11821 gaagtggttt ttaggcggga aaattagccc ccctcttttt atatctcttt ttaaacctct
    11881 ttttaaacct ctttaccgta agctgtgtcc ttactctacc aatggataca gaatgttatg
    11941 aaggacattt tgtctgttta tgaaggacat tttgtctgtt tatgaaggac attttgtctg
    12001 tttatgaagg acattttgtc tgtttgaacg tcaaatatga atgtcttgac atttaaaaaa
    12061 tgtcattcat aattggattg aggtgattat
//
