LOCUS       NZ_LR135269             1928 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 12.
ACCESSION   NZ_LR135269
VERSION     NZ_LR135269.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135269.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="12"
     gene            <1..668
                     /locus_tag="EQB59_RS15500"
     CDS             <1..668
                     /locus_tag="EQB59_RS15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_164856674.1"
                     /translation="QFHEGKARIDFNPNKIGQFLASSMKNFIHDLFLEPHFSRADIACD
                     MVNVPDEFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKV
                     VPKEVETWWRLEMQLRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQ
                     NFWSLLSRNSKYKYRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            868..>1050
                     /locus_tag="EQB59_RS15505"
                     /pseudo=""
     CDS             868..>1050
                     /locus_tag="EQB59_RS15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            1631..>1928
                     /locus_tag="EQB59_RS15515"
                     /pseudo=""
     CDS             1631..>1928
                     /locus_tag="EQB59_RS15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
ORIGIN
        1 tgcaatttca tgaagggaaa gcacgaattg attttaatcc caataaaatt ggtcagtttt
       61 tagccagttc gatgaaaaat ttcattcatg atttatttct agaaccccat ttttcgcggg
      121 cagatatcgc ttgcgatatg gttaatgttc cggatgagtt tattacacaa tatcgagtgg
      181 ttgatcctgt ttctttcaag ccaatttatg ggcggtctgg gaagctggaa acagcttatt
      241 ggggcagtcg tgccagcgaa cgccagattc ggctatacaa caaaaaattg gaacaggaaa
      301 ccaaacgaaa agttgtacca aaggaagttg agacttggtg gcggttagaa atgcaactac
      361 ggcgtggcaa ggcaaccgat tggcataata tggttcaaga aagtttgaat agttttgcga
      421 gcctgcattt tttgccgatt gatacaacac ctacagatag aataatgctc gctggtttaa
      481 tgtctgatca aaatttctgg tctcttttgt cgaggaattc aaaatataaa tatcgggaat
      541 tgctgaaaga agaaagtcaa aatgatgaat tgacgaatca tttgcgagaa acgtttgcag
      601 aatcggcaga caatttgaaa caagaattag acacgtggct tttgggtttg gacgtgacag
      661 agaagtgata ccgaataaga aaagtcagat gagtgagagt aaatttacgt caatgtttaa
      721 tgacgtgagt ttacgataag cgattatttt gcgtgaagca aaataaagcg taaaaggagt
      781 tgagagcgtg acacgctttc ggctcctttt ttcgttgtca cttgtgacag cgaataccct
      841 cggagagcat ttggaattgt gacagccatg tcacaattag gaaaatgtta ctctttgtca
      901 aagagtaaca gaaaggttta cactcttttt attagcgaca gcgcgaagga ggggtttaac
      961 ttggggttta gtatttcgtt caaaaaggga acaaaaaata caagcatttc tcataataat
     1021 cgtgagttaa ctgatagcca aaaaatcaat agtttgtctg gtagaaaata attagcagat
     1081 ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca tttcaaacta tattttctac
     1141 aatagctcta aaatggcata ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt
     1201 tatcggcaca atcggtgaaa cacgttagag gagcgaaaat ggagcctgta aggcgactgg
     1261 tagcggaacg gtcgtgttag attgtgtagc cgatagctga acgaaataaa tgctggcagc
     1321 gccattctat ttcggcagga ggaggctcaa ggggttcggg gaatgaaatt ccctgaggtc
     1381 tttttatttt ttctagagga gtgctataat caaagaaaat gagaaaaaag agtaaaaaaa
     1441 tagtcgagca gtgttgacgc actctcgacc tttacggaaa ctacttcacc tagttccgta
     1501 actcaagttc attatacgaa tatttttagg tttcttcaag ctagagattt ttggatgtgg
     1561 atttttagag ttacgtgaac tctggaaatt ctttccaaga atctcttttt tcgttgagga
     1621 ggagctattt ttgagtaaaa aaggcataat cgtaaatgag tgggaaaaac ttaaccgtat
     1681 ttctaacagg cggttaaatg ttcaaaattt gaacaccccc ctaaaattac agtggtcaat
     1741 cgaccgaatt accattgttg gaaaattgaa agaaaatatt tattatcaca caccaaatga
     1801 tgtcttgatt ttaaattttg agcaattgat gcaattgaat gaggggaatg ggtttgtcaa
     1861 atcagttggt aaaaatggct ggcaaattgt cgaccaatac gaagaaaata tcgcgtatat
     1921 tgagctct
//
