LOCUS       NZ_LR135268             1955 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 11.
ACCESSION   NZ_LR135268
VERSION     NZ_LR135268.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135268.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="11"
     gene            complement(<1..192)
                     /locus_tag="EQB59_RS15480"
                     /pseudo=""
     CDS             complement(<1..192)
                     /locus_tag="EQB59_RS15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(<800..982)
                     /locus_tag="EQB59_RS15490"
                     /pseudo=""
     CDS             complement(<800..982)
                     /locus_tag="EQB59_RS15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1182..>1955)
                     /locus_tag="EQB59_RS15495"
     CDS             complement(1182..>1955)
                     /locus_tag="EQB59_RS15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364959.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127837504.1"
                     /translation="QLMRLNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKFHEGKGRI
                     DFNPNKINQFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPVSFKPI
                     YGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRGKATDW
                     YAMVHESLDSFASPHFLPVSTKPIDKVLISGLIADQNNWSIISRDLKYRLRKLLKQESQ
                     NDELTNHLRESFAESADNLKQELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(1236..1949)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.736"
                     /coverage="89.0"
                     /evalue="0.0"
ORIGIN
        1 ttcaaaatct aaaatcaaca catcattttg cgtgtggtaa taaatatttt ccttcatttt
       61 tccaacaatg gtaattcgat caattgacca agacattttt atcgttttta cgctctcacc
      121 gacacttaac cgcctgttag atatacggtt aagtttttcc cactcattta caatcgtgcc
      181 atttttactc aaatttcgtt cctcctcaaa gaaaaaagag attcttggaa agaattttaa
      241 aagttcacgt aacccttaaa attcattaac aagaatctct agtcttgaag aaacctaaaa
      301 atattcgtat aatgattatg agttacggga ctagtagaag tagtttccgt aaaggtcgag
      361 aacgttggta gcgttgctcg actatttttt tgctcttttt tcgatttttt ataattataa
      421 catcagaaga caaaaactga caaatactcc cgagtataaa agacacaaga ccttggggaa
      481 tgtgattccc caaacccctt gagcctcctc ctgccgaaat ggaagggcgc taccgctgtt
      541 tatttcattc agctatcggc tttcacaatc taacacgacc gttccgctac cagtcgcctt
      601 acaggctcca ttctcgctac tctaacgtgt tttaacgatt gtgccgataa ctaaacgaaa
      661 taaacgctaa aaccgctcag aacgcaaaat atgccgtttt agagctattg tagaaaatgt
      721 agtttgaaat gcaattttct tctttcagct ttttatttcc cacttgtaaa tctgctaatt
      781 attttctacc agacaaacca ttgatttttt ggctatcagt taactcacga ttattatgag
      841 aaatgcttgt attctttgtt ccctttttga acgaaatact aaaccctaag gtgaacccct
      901 ccttcgcgct ctcgctaata aaaagagtgt aaacctttct gttactcttt gacaaagagt
      961 aacgttttcc taattgtgac atggctgtca caattccaaa cgctctccga gggttttcgc
     1021 tgtcacaagt gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac
     1081 gctttatttt gcttcatgca aaatattcgc ttatcgtgaa tttacgtcac taaacattga
     1141 cgcaaatcca ctctcactca tctgactttt cttattcggt atcacttctc tgtcacgtcc
     1201 aaccccaaaa gccacgtgtc taattcttgt ttcaaattgt ctgccgattc cgcaaatgat
     1261 tctcgtaaat gattcgtcaa ttcatcattt tgactttctt gcttaagcaa ttttcttaat
     1321 ctatatttca aatctcgact gataatactc caattatttt gatcagcaat caatcctgaa
     1381 atcaacactt tatctattgg tttagtactc actggtaaaa agtgtggact ggcaaagcta
     1441 tctaaacttt catgaaccat cgcataccaa tcagtagctt ttccgcgccg taactgcagt
     1501 tccagtcgcc accaagtttt tatttccggt ggtacaattt tccgcttagt ttcttgttcc
     1561 aattttttgt tgtacatccg tatttgtcgc tcactggcac gacttcccca ataagccgtt
     1621 tccagcttcc cagaccgccc atagattggt ttgaacgaaa caggatcaac cactcgatat
     1681 tgtgtaataa actcatcagg cacatcgata atatcgcaag caatatctgc acgtgagaaa
     1741 tgtggttcca aaaataaatc atgaatgaaa tttttcattg aactagctaa aaattgattg
     1801 attttatttg ggttaaagtc aatccgtcct tttccctcat gaaacttaag aatctcaata
     1861 taagcaatat tttcttcata ttggtctaat aattgccagc cattattccc aaccgctttt
     1921 aaataaccat taccttcgtt tagacgcatc agttg
//
