LOCUS       NZ_LR135267             1981 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 10.
ACCESSION   NZ_LR135267
VERSION     NZ_LR135267.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135267.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            complement(<1..231)
                     /locus_tag="EQB59_RS15465"
                     /pseudo=""
     CDS             complement(<1..231)
                     /locus_tag="EQB59_RS15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /name="replication protein"
     gene            complement(<814..996)
                     /locus_tag="EQB59_RS15470"
                     /pseudo=""
     CDS             complement(<814..996)
                     /locus_tag="EQB59_RS15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(1196..>1981)
                     /locus_tag="EQB59_RS15475"
     CDS             complement(1196..>1981)
                     /locus_tag="EQB59_RS15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127837502.1"
                     /translation="VDFEQLMRLNENQGYLKSVGTNGWQLFDQYGEDIAYIEMLKFHPD
                     KGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIACDILGVPDDFITQYRIVDPVS
                     FKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQERKKQIVPKEIETWWRIEMQLRRGK
                     ATDWHAMVRESLDSFASPHYLPGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLK
                     EESQNDELTNHLRETFKESANDLKIELDTWLLGLDVTEK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(1197..1963)
                     /name="rep_cluster_1968"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaacacaact ccattttgcg tccaataagc tatggtatca ttcagttttc cgacgattgt
       61 aattcgatcg attgaccaac ttaattttag ggggtgtacc ttatcgtcaa cacttaaccg
      121 cctgttagaa atacggttaa gtttttcgag ctgatttatc gttgttcttt tttctgcccc
      181 tcgaccttca atttttgatt gaatttcact caactgattt tcatttgcca ttttcgtcct
      241 ccagacgaaa aaagagactt ttggaaagaa tttccagagt tcacgtaacc ctaaaaattc
      301 atatccaaaa atctctagct tgaagaaacc taaaaatatt cgtataatga acctgagtta
      361 cggagctagg tgaagtagtt tccgtaatta gccgaagggt gcttccaaca ccgttcggct
      421 tttattttgc tcttttttct cgttttcttt gattatagca ctcctcctaa aaaaataaaa
      481 agacctcagg gaatttcatt ccccgaaccc cttgagcctc ctcctgccga aatggaaagg
      541 gcgcacccgc agtttatttc attcagctat cggctttcac aatctaacac gatcgttccg
      601 ctaccagtcg ccttacaggc tccattttcg ctcctctaac gtgttttaac gattgtgccg
      661 ataactaaac gaaataaatg ctaaaaccgc tcagaacgca aaatatgccg ttttagagct
      721 attgtagaaa atgtagtttg aaatgcaatt ttcttctttc agctttttat ttcccactta
      781 taaatctgct aattattttc taccagacaa accattgatt ttttggctat cagttaactc
      841 acgattatta tgagaaatgc ttgtattctt tgttcccttt ttgaacgaaa tactaaaccc
      901 taaggtgaac ccctccttcg cgctgtcgct aataaaaaga gtgtaaacct ttctgttact
      961 ctttgacaaa gagtaacatt ttcctaattg tgacatagat gtcacaattc caaacgctct
     1021 ccgagggttt tcgctgtcac aagtgacaac gaaaaaagga gccgaaagcg tatcacgcta
     1081 tcaactcctt ttacgcttta ttttgcttca cgcaaaataa tcgcttatcg taaattcacg
     1141 tcattaaata ttgacgtaaa tttactctca ctcatctgac ttttcttatt cggtatcact
     1201 tctctgtcac gtccaaaccc aaaagccacg tgtctaattc tattttcaag tcgtttgcag
     1261 attccttaaa cgtctctcgt aaatgattcg tcaactcatc gttttggctt tcttctttca
     1321 acaatttgcg caatctatat ttcaaatttc tgctaatata cgcccactct gaatgatctt
     1381 gattcagccc ttttatcata attctatcaa caggttttac atctccaggc aagtaatgag
     1441 gacttgcaaa gctatctaaa ctttcccgaa ccatcgcatg ccaatctgtt gctttgccac
     1501 gtcttagctg catttctata cgccaccatg tttcgatttc ttttggtaca atctgttttt
     1561 ttcgttcctg ttcaagcttt ttgttgtaca tccgcacttg tcgctcgctg gcacgactac
     1621 cccagtacgc tgtttctaat ttgccagaac ggccataaat tggcttaaaa gacacagggt
     1681 caacgattcg atactgggta ataaaatcat caggaacacc gagaatatcg caagcaatat
     1741 ctgcacgtga gaaatgcgca tccagaaaca aatcatgaat gaattgtttc atcgacccct
     1801 ttaaaaactc gccaatttta tttggattaa aatcaatacg acctttgtca gggtgaaatt
     1861 ttaacatttc gatataggcg atatcttctc catattggtc aaataattgc caaccatttg
     1921 taccaaccga cttcaaatac ccttgatttt cattcaaacg cattaattgc tcaaaatcga
     1981 c
//
