LOCUS       NZ_LR135266             3848 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 9.
ACCESSION   NZ_LR135266
VERSION     NZ_LR135266.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135266.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            complement(155..1561)
                     /locus_tag="EQB59_RS15445"
     CDS             complement(155..1561)
                     /locus_tag="EQB59_RS15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_127837500.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSAEMRYIYRQREENEREK
                     ALVASERAEIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQHK
                     LERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            complement(1783..2145)
                     /locus_tag="EQB59_RS15450"
     CDS             complement(1783..2145)
                     /locus_tag="EQB59_RS15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098379973.1"
                     /translation="MGVTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAEMGKALRKHF
                     GFESFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYLNESDGLFEVIPNNKSQ
                     MSESKFTSMFSDVDLR"
                     /name="hypothetical protein"
     gene            complement(2198..2446)
                     /locus_tag="EQB59_RS15455"
     CDS             complement(2198..2446)
                     /locus_tag="EQB59_RS15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_213067874.1"
                     /translation="MKLDKQRLYEAVKDNCNCGGNRQLFKAMMVTVYRRLDGENAEAAV
                     ENAYFQVFGEHHSPEWDRAILDDMAEMHEKLWRALND"
                     /name="hypothetical protein"
     gene            complement(2433..3413)
                     /locus_tag="EQB59_RS15460"
     CDS             complement(2433..3413)
                     /locus_tag="EQB59_RS15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355134.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_127837498.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPRMRELMTGCGSY
                     LEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIVEEKQYQFIFMT
                     LTTPNVKADKLNEEIDLFNHALSKMFRRKRVTNVIKGYVRKLEITYNKKRDDYNPHFHL
                     ILAVPKHYFTVKKYYIKQSEWLDLWRDVTGKTGINPDGTDEITQLDVRKVKGFQQEKAV
                     QEVAKYSAKDFEMTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYEAGKLDHYKKKDE
                     NEYFWFLTASWMQKDANYSVAVRELTDDEKKFFAERYDKEGDETG"
                     /name="protein rep"
     Typing          complement(159..1561)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.735"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaagggcgc ccccaaactt cgctaacgct aagttggcca aaaaatggac ttgccatttt
       61 ttagcaaatg tcagcttcag ggattagtgg tttagccgtc aactccttat cctcggcaca
      121 aaccgcctcc ggtaccgttg actgcacatc gtaactacca ctcgatctca tcttcagaac
      181 gctctaattt atgctgtgag acgttttgac gataaaataa ggttttctcc tgctctaata
      241 cttctaattc ctcagaaagc cccatatcct tttctgcaag cccaaaacgg atagacaaac
      301 gagtgatcga tttttgaaat gcgtttttta atcgctctaa acgctggcgt aaagagcgat
      361 tttcttcttt cagcttttta ttttccactt gtaaatctgc taattgtttt ctaccagaca
      421 aatgacgctc ttcttccaat gcagctacat actcatttaa ttctgtattt tgctcaagca
      481 tctgtaaata ttgctcactc atgttatcag catttctctt ggcatttgtt gctctcgtag
      541 ctcgttgata caaatcaaca aaatcattcc cagataaatg atacgaacca tcaggaagcc
      601 tcttgaaact ttctaaagga accatatctt tttggaaaat gaccgccatc tcttttttta
      661 cattcagcat atttttcttc tcttcttcaa tctccgctct ctcagaagcc accagagcct
      721 tctcacgctc gttttcttct ctttgcctat aaatatacct catttccgca gaaacgctct
      781 cacgctcttt tacagccgtt tggcgcaatt cagaaatttc actcatgatt tctttgtatt
      841 cgtgaatatc tttaattttg ttggtaccga caatatccct tttccaaccg taatcattca
      901 ttaatcgttc tatagagtca acttcttgat ttctaaaatt attgaaaagc actcgaccat
      961 tatctttatc agcgtgtatc ccttgttgaa ccagtgcttt attaaagctc ggctgctttt
     1021 gaacaccccg tttataacct tcagcaaccg gcacaatatt caagtgcagg tgcggactta
     1081 tttcgtcatt atgaataaca gcattataaa cagccaaatt agggtttctc tcttgaaact
     1141 ccttgtaata ctgaaccaaa attttattgg caatcactcg attttgttca gcttgttgtt
     1201 tcgatatgcc agtagaagaa ccatctgctc gatttgtctg gaaatcatct aaaatgccta
     1261 tttgcacaat aaattctcgt tgaggttcca actttttatc cttgcgcact ttcgctaaat
     1321 agtcctcgat ttttctatcg gctctttttt gtttcgcatt atatttttct accacttcgc
     1381 caaacaattc ctcatattta tctgctagat tcgtctgctc taaataaata ttttcatccg
     1441 atttcgtgaa gtcaatgtgc ttatgccaat cattggtttt ctggcgatca gttaactcac
     1501 gattattatg agaaatactt gtattcttcg ttcctttttt aaatgaaata ctaaacccca
     1561 agttaaaacc ctccttcgcg ctgtcgctaa taaaaagagt gtaaaccttt ctgttactct
     1621 ttgtcaaaga gtaacatttt cctaattgtg acatggctgt cacaattcca aatgctctcc
     1681 gagggtattc gctgtcacaa gtgacaacga aaaaaggagc cgaaagcgtg tcacgctctc
     1741 aactcctttt acgctttatt ttgcttcacg caaaataatc gcttatcgta aatccacgtc
     1801 actaaacatt gacgtaaatt tactctcact catctgactt ttgttattcg gtatcacttc
     1861 gaataagccg tcagattcgt ttagataatc tcgttcgctt ggcgctgatt tttcatcagc
     1921 aagccactca ttatccactg atttttcgtg ttgctgatcg tcagaaatcg acgatagcca
     1981 ccgctcaaaa tcagcaaaag attcaaaccc aaaatgcttg cgaagcgcct tgcccatttc
     2041 agcattttcc ttctcaatca atcgcttttt ctgttggcga agttttgcct gctctttttt
     2101 caatttttct tcttgtgctt taatatcagc taaagttact cccatgaatt acaactcctt
     2161 acccttagat tcattcctat aaataacacc aatttgatta gtcatttagc gccctccaca
     2221 atttctcgtg catttccgcc atatcatcaa gaatagcacg atcccattca gggctatggt
     2281 gttcaccgaa cacttgaaaa taagcatttt ccacagctgc ctcagcattt tccccatcca
     2341 accgacgata aacagtcacc atcatagcct taaaaagctg acgatttccg ccacaattac
     2401 aattatcttt caccgcttca tatagccttt gcttatccag tttcatcacc ctccttatca
     2461 tagcgttccg caaagaattt cttctcatca tccgtcaact cacgaacggc caccgagtaa
     2521 ttggcatcct tttgcatcca gctagcagtc aaaaaccaga aatattcgtt ctcgtctttt
     2581 ttcttgtagt gatcgagctt gcctgcttcg tatttcttct tgtactcctt gaagaccccg
     2641 ttaaacgtga ttaactggcg ccctttcata gcatgataga atgtgtcaaa cacttcttga
     2701 ctctcggtca tttcaaaatc ttttgccgaa tattttgcaa cttcctgtac tgctttttct
     2761 tgttggaatc ctttgacctt acggacatct aactgagtta tttcgtctgt gccatcagga
     2821 ttaattcccg ttttcccagt cacatcacgc cataaatcaa gccattccga ctgcttgata
     2881 taatattttt tgacagtgaa atagtgttta ggaaccgcca aaatcaaatg aaaatgcgga
     2941 ttatagtcat cacgtttttt gttatacgtg atttccaatt tccggacgta ccccttgatt
     3001 acattagtaa ctcttttcct acgaaacatt ttcgatagcg catgattaaa taaatcaatt
     3061 tcttcgttta gcttgtccgc cttcacatta ggcgttgtta acgtcataaa aataaattga
     3121 tactgctttt cttccacaat ggcttgcatc ataatcgaca acatcatcga atccttacga
     3181 gctttacgcc aagcacataa cggacagaaa cgatttttac aaaagtgagc ttggaccaat
     3241 ttctttttct ccttatcaat cgttgcaata aattctaaat atgaaccaca gcctgtcatc
     3301 aattcacgca ttctgggaga aattcgatta tcactaaatg ccaccacttt tttcaaattt
     3361 ttcttttttt ctcgaaatgt tccgttaatc aaatcttgct ttttcttttt catcttgcta
     3421 tactgaatct acaaattttg tatacaaaaa aggctgaaaa gccgataaca aaaattaaga
     3481 ttgctctcca ttctgattta gttttttttt agtcaactaa aactataaat caaccatatt
     3541 ggaaaagcaa tctttttttt tgttctccct tagagccaca agggatagac ccatactata
     3601 gccccgattt tctgccgaaa atagttacct tatgtatcaa gataagaaaa aacatcaaaa
     3661 aagagggcga aaatcgcccc ctttttttcg ttttaaaacc ccaaaaccca aaggcataaa
     3721 tttcggtcaa ttgaccactg aatttttagg ggggtgtcca taaaagggac acttaaccgc
     3781 ctgttagagg gggctgttaa tttttcccac tcattcttag tcatgcaaac ataaaaccgc
     3841 acccttta
//
