LOCUS       NZ_LR135265             4374 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 8.
ACCESSION   NZ_LR135265
VERSION     NZ_LR135265.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135265.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            <1..988
                     /locus_tag="EQB59_RS15410"
     CDS             <1..988
                     /locus_tag="EQB59_RS15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_127837496.1"
                     /translation="EFQERNPNLAVYNAVIHNDEISPHLHLNIVPVAEGYKRGVQKQPS
                     FNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMNDYGWKRDIVGTNKIKDIHEYKE
                     IMSEISELRQTAVKERESVSAEMRYIYRQREENEREKALVASERAEIEEEKKNMLNVKK
                     EMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQRATRATNAKRNADNMSEQYLQML
                     EQNTELNEYVAALEEERHLSGRKQLADLQVENKKLKEENRSLRQRLERLKNAFQKSITR
                     LSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRKLERSEDEIEW"
                     /name="plasmid recombination protein"
     gene            1435..2403
                     /locus_tag="EQB59_RS15415"
     CDS             1435..2403
                     /locus_tag="EQB59_RS15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002298422.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNMYTPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKWQD
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKIVPPEIETWWRLELQLRRG
                     KATDWHAMVHESLNSFASPHFLPLDVKTNDKVMIFGLIANPEMWGFLERRMKYKLRDIL
                     KRESQNDELTNHLRETFSESADVLKKELDTWLQGLDVTEEE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2406..2639
                     /locus_tag="EQB59_RS15420"
     CDS             2406..2639
                     /locus_tag="EQB59_RS15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298421.1"
                     /translation="MAEKQKHFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYVYGVAESNGEDND"
                     /name="hypothetical protein"
     gene            2632..3051
                     /locus_tag="EQB59_RS15425"
     CDS             2632..3051
                     /locus_tag="EQB59_RS15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298420.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            3060..3368
                     /locus_tag="EQB59_RS15430"
     CDS             3060..3368
                     /locus_tag="EQB59_RS15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298419.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            3372..3734
                     /locus_tag="EQB59_RS15435"
     CDS             3372..3734
                     /locus_tag="EQB59_RS15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298418.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            3956..>4374
                     /locus_tag="EQB59_RS15440"
     CDS             3956..>4374
                     /locus_tag="EQB59_RS15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_197724955.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYY"
                     /name="plasmid recombination protein"
     Typing          1519..2393
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.032"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3..985
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.207"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3864..4372
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.412"
                     /coverage="99.0"
                     /evalue="1.41e-105"
ORIGIN
        1 ggagtttcaa gagagaaacc ctaatttggc tgtttataat gctgttattc ataatgacga
       61 aataagtccg cacctgcact tgaatattgt gccggttgct gaaggttata aacggggtgt
      121 tcaaaagcag ccgagcttta ataaagcact ggttcaacaa gggatacacg ctgataaaga
      181 taatggtcga gtgcttttca ataattttag aaatcaagaa gttgactcta tagaacgatt
      241 aatgaatgat tacggttgga aaagggatat tgtcggtacc aacaaaatta aagatattca
      301 cgaatacaaa gaaatcatga gtgaaatttc tgaattgcgc caaacggctg taaaagagcg
      361 tgagagcgtt tctgcggaaa tgaggtatat ttataggcaa agagaagaaa acgagcgtga
      421 gaaggctctg gtggcttctg agagagcgga gattgaagaa gagaagaaaa atatgctgaa
      481 tgtaaaaaaa gagatggcgg tcattttcca aaaagatatg gttcctttag aaagtttcaa
      541 gaggcttcct gatggttcgt atcatttatc tgggaatgat tttgttgatt tgtatcaacg
      601 agctacgaga gcaacaaatg ccaagagaaa tgctgataac atgagtgagc aatatttaca
      661 gatgcttgag caaaatacag aattaaatga gtatgtagct gcattggaag aagagcgtca
      721 tttgtctggt agaaaacaat tagcagattt acaagtggaa aataaaaagc tgaaagaaga
      781 aaatcgctct ttacgccagc gtttagagcg attaaaaaac gcatttcaaa aatcgatcac
      841 tcgtttgtct atccgttttg gtcttgcaga aaaggatatg gggctttctg aggaattaga
      901 agtgttagag caggagaaaa ccttatttta tcgtcaaaac gtctcacagc gtaaattaga
      961 gcgttctgaa gatgagatcg agtggtagtt acgatgtgca gtcaacggta ccggaggcgg
     1021 tttttgccga ggataaggag ttgacggcta aaccactagc ctattaagct gacatttgct
     1081 aaaaaatggc cagtccattt tttggccaac ctagcgttag cgaagtttgg gggcgccctt
     1141 ttcaaagggt gcggttttat tttttggata tttgcatagc taaagagaag tgctattatt
     1201 gagttagcaa acaaatgaac tgaaaaaaga gtaaaaaaat agtcgagcaa cgctaccaac
     1261 gttctcgacc tttacggaaa ctaatcgcac tagctccgta acttcttaat cattatacga
     1321 atatttttag atttcttcaa gactagagat tttttggtaa tgaattttta agagttacgt
     1381 gaacttttga aaattctttc cagaagtctc tttttctttg aggaggaaca aaatttgagt
     1441 aaaaatggca taatcgtaaa tgagtgggaa aatttaacag ccctatctaa caggcggtta
     1501 aatgtacaaa atatgtacac ccccttaaaa atgcagtggt caattgaccg aattactatc
     1561 gttggaaaac tgaaggaaaa tatttattat cacactcaaa atgatgtgtt gattttagat
     1621 tttgagcaat taatgcgggt gaatgaaggc aatggctatt taaaagcggt tgggaataac
     1681 ggctggcaat tattagacca atacgaagaa aatattgctt atattgagat tttgaaatgg
     1741 caagatggca aagggcggat tgattttaac ccgaataaga ttggtcattt tttagctagt
     1801 tcaatgaaaa atttcattca tgatttattt ctagaaccac atttctcacg tgcggatatt
     1861 gcttgcgata ttatcgatgt gcctgatgag tttattacac aatatcgagt ggttgatcct
     1921 gtttctttca agccaattta tgggcggtct gggaagctgg aaacagctta ttggggcagt
     1981 cgtgccagcg aacgccagat tcggctatac aacaaaaaat tggaacagga aaccaagcgt
     2041 aaaattgtgc caccagaaat agaaacttgg tggcgactgg aattgcagtt aaggcgtggg
     2101 aaagcaacag attggcatgc aatggttcat gaaagtttaa atagttttgc tagccctcat
     2161 tttctaccac ttgatgtaaa aacaaatgat aaggtgatga tttttggact gattgctaac
     2221 cctgaaatgt gggggttttt agagcgtaga atgaaataca aattgagaga tattttgaaa
     2281 agagaaagtc aaaatgatga gttgacgaat catttacgag aaacattttc ggaatcagca
     2341 gatgttttga aaaaagaatt agacacttgg cttcaagggt tagatgtaac ggaggaggaa
     2401 taagaatggc agaaaaacaa aagcattttg gtaagaaaaa ttggttggtg aatttaacag
     2461 gtgataacgt tgtcgttgtt tttttggata gcaaaaaaat gtccggtaag ttacttgatg
     2521 taacgcagta tgagttgatt ttgaaaaata ataaaattga ttccccaatc attatcccta
     2581 agcatgctgt taaatatgtt tatggggtag ctgaaagtaa cggagaagat aatgactgat
     2641 ttgccagaaa aaatgaccat aaaaaacctt gctgatgagt tgggtatatc taaaaataca
     2701 gtgcgcaatc aactggtcaa tgcaggttat gaccttgctc ggtataaaga taatggggtt
     2761 atcatgttag acaaggaatt gattggtgtt gtgcggtcaa tttatcggtc aaaaggtggt
     2821 caattgactg gtgatttacc ggtcaatgat gcaggcaatg atttcccgtt agaaaaagaa
     2881 aatgaatttt tgcgtgaaca attagcggaa aaagataggc atatttctga attaacggaa
     2941 atgattcgtg accaacaaaa attgactttg cttcagcaac aaaaaaacga tcagttgtta
     3001 ctagaaaatc aagaattaaa aaatcgaaaa tggtggcaat tctggaagtg aggtgtgaca
     3061 tggataaaat tgatttgaga gaagaaacag tcgatagaat tgaattgatg tatgaacggt
     3121 tagctgatta tttgaatttg ccggatgaag ataattatag tgaaatgctt gaacgaatga
     3181 tggatgttct caactcggtt atcaatgctg atctagcaaa atcttcagtt ttgactaatg
     3241 aatttgttca taagcattta gcgcaaacaa ctgatgaatc agaaaaagaa ttttggttga
     3301 aattaatgag tcatgttatg cagttaaacc aaatttccgt tattaattcc aaagaaaagg
     3361 agatgtagtt tatgggaatg actttagcag atattaaagc gcaagaagaa aaattaaaaa
     3421 aagaacaagc gaaacttcgc caacagaaaa aacgcttgat tgagaaagaa aatgctgcaa
     3481 tgggcaaggc tcttcgcaag cgttttgggt ttgaaacttt tgctgatttt gagcggtggc
     3541 tatcgtcgat ttctgacgat cagcaacacg aaaaatcagt ggataatgag tggcttgctg
     3601 atgaaaaatc agcgccaagc gaacgagatt atcccgatga atctgacggc ttattcggag
     3661 tgataccgaa taagaaaagt cagatgagtg agagtaaatt tacgtcaata tttaatgacg
     3721 tgaatttacg ataagcgatt attttgcgtg aagcaaaata aagcgtaaaa ggagttgaga
     3781 gcgtgacacg ctttcggctc cttttttcgt tgtcacttgt gacagcgaat accctcggag
     3841 agcatttgga attgtgacag ccatgtcaca attaggaaaa tgttactctt tgacaaagag
     3901 taacagaaag gtttacactc tttttattag cgacagcgcg aaggagggtt ttaacttggg
     3961 gtttagtatt tcatttaaaa aaggaacgaa gaatacaagt atttctcata ataatcgtga
     4021 gttaactgat cgccagaaaa ccaatgattg gcataagcac attgacttca cgaaatcgga
     4081 tgaaaatatt tatttagagc agacgaatct agcagataaa tatgaggaat tgtttggcga
     4141 agtggtagaa aaatataatg cgaaacaaaa aagagccgat agaaaaatcg aggactattt
     4201 agcgaaagtg cgcaaggata aaaagttgga acctcaacga gaatttattg tgcaaatagg
     4261 cattttagat gatttccaga caaatcgagc agatggttct tctactggca tatcgaaaca
     4321 acaagctgaa caaaatcgag tgattgccaa taaaattttg gttcagtatt acaa
//
