LOCUS       NZ_LR135262             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4457 plasmid 5.
ACCESSION   NZ_LR135262
VERSION     NZ_LR135262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884735
            Assembly: GCF_900639405.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:06:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,952
            CDSs (total)                      :: 2,862
            Genes (coding)                    :: 2,745
            CDSs (with protein)               :: 2,745
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 63 of 117
            Pseudo Genes (incomplete)         :: 51 of 117
            Pseudo Genes (internal stop)      :: 27 of 117
            Pseudo Genes (multiple problems)  :: 20 of 117
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135262.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4457"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(22..192)
                     /locus_tag="EQB59_RS15670"
     CDS             complement(22..192)
                     /locus_tag="EQB59_RS15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(208..747)
                     /locus_tag="EQB59_RS15270"
     CDS             complement(208..747)
                     /locus_tag="EQB59_RS15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(775..1512)
                     /locus_tag="EQB59_RS15275"
     CDS             complement(775..1512)
                     /locus_tag="EQB59_RS15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            2157..2243
                     /locus_tag="EQB59_RS15280"
     CDS             2157..2243
                     /locus_tag="EQB59_RS15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(2364..3053)
                     /locus_tag="EQB59_RS15285"
     CDS             complement(2364..3053)
                     /locus_tag="EQB59_RS15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3055..3333)
                     /locus_tag="EQB59_RS16030"
     CDS             complement(3055..3333)
                     /locus_tag="EQB59_RS16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(3293..4207)
                     /locus_tag="EQB59_RS15295"
     CDS             complement(3293..4207)
                     /locus_tag="EQB59_RS15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4189..4569)
                     /gene="mobC"
                     /locus_tag="EQB59_RS15300"
     CDS             complement(4189..4569)
                     /gene="mobC"
                     /locus_tag="EQB59_RS15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(5011..5148)
                     /locus_tag="EQB59_RS15675"
     CDS             complement(5011..5148)
                     /locus_tag="EQB59_RS15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(5262..5882)
                     /locus_tag="EQB59_RS15305"
     CDS             complement(5262..5882)
                     /locus_tag="EQB59_RS15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(5879..8005)
                     /locus_tag="EQB59_RS15310"
     CDS             complement(5879..8005)
                     /locus_tag="EQB59_RS15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(8059..8433)
                     /locus_tag="EQB59_RS15315"
     CDS             complement(8059..8433)
                     /locus_tag="EQB59_RS15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            8566..9027
                     /locus_tag="EQB59_RS15320"
     CDS             8566..9027
                     /locus_tag="EQB59_RS15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     BGC             3059..9317
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(776..1512)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3297..4207)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcggaagagc agtctttttt attatttatc cgtctctttt acaaacaatt cttcgttagg
       61 actgttgtaa taatttggat tcaatttcaa ttcaccatgt ttattccctt tattgattac
      121 tgacaataac atcggactga agcctagaat tcctagtaaa atagaaccga atagaagatt
      181 ttttcttgtc atgaatgttc cctccagtta tttccaaagt tgccaccatt tttgcttctt
      241 cttttttgtg tgtttttcta tcgattctgt ttcttcagaa agaatagtag ttgattcatc
      301 ttctggagtt tcttccgtat aagctaattg taactgttct tgcagttttt cgatctgttg
      361 atttgcctgt agggtgagct gttgttgctg gtcgagtaat ttttgcatat tagaattatt
      421 ttgctctttg agggaaagtt gttctcgtaa aaattcattt tcttcacgca acttatctag
      481 caactcgttt acaatcacgt ttttgcttga tatataaacg tttttatcgt tttcttgttc
      541 atactcatgc aacctatata gtaacctttg gttacctaag ttgctagcaa gttgcaatac
      601 tccagaacgt aatacaacct tataaccacg ctcattttta gctaataagg acgggtctaa
      661 tttatcaaca tgtttagaga ttgcttgcct acttatttca aataattcag ataattcctt
      721 tattgtaaag gtttctttgg gttgcatgat aattgcctcc taaattgtta ggtattattt
      781 ctctaaccaa ttatctaagg taattgaagg tatattttct tcaaaataaa tttgcagaga
      841 tcctatctta tttcctttgt aggctttaat ttttttgatt tcaaaagttt taaaaatagc
      901 agggtgagtt gaattaacgt ttaaaaattc ttttttcgct tgggcaaaaa tttgtttatc
      961 aatatctctc atgcgataac tttctggtat tgctaataga gctctaaaat cttctatttt
     1021 tacaaaccag tgaccttttt tagatcttct gtctttatga gccataagtt ggcgatataa
     1081 ctctttaacg tagctactct ttaagtttgt catattttct aattcgaaac gagtaaaatt
     1141 agttccgatt gaattaagta tatattcaaa ttcttcagaa acctttatta ataatgtttc
     1201 attcacttta tcgattttaa atttttgaaa cagatttaat tgttcatatg ttttttcgtc
     1261 ttcataaata aaagacaaag taatgatttt ttttgacatt tttattaaat cattagcaaa
     1321 acggtcgcca cgtgcattta tattgtaatc aattaattct tttatttgtt taaatgtaaa
     1381 aatttcttct tttgtacctt ttcttttcat tttagaacat attccccaaa ataaatccat
     1441 atctataggg gtaaattttc ttaaagggac agaatttaat ccattattat atcgaactat
     1501 atcgttactc aataaaaatc acctcaataa attatgttat catagacata agaggatgta
     1561 aaacacaatt tatcccccat agtgtgacaa tttatccccc atagtgtgac aatttatccc
     1621 ccatagtgtg acaatttatc ccccatagtg tgacaattta tcccccacta cctatgtcaa
     1681 tacttactct cccataaggt tgctaatccc taacagttat tattaacagt atatctaaca
     1741 gtatatctaa cagtatatct taagagcaca ttgaatttcc cgcctaaaaa actcctactt
     1801 tttcctccaa aaaggttcgc gaatttttct aaactggaaa acttgctccc tacggtcgct
     1861 aaaaaggtgt gatcgctatg ctctcatatt atactgcgca gtcgcctccg gctcctttgc
     1921 taaaaccgtt gttcaaagag gaggtgtgag actctgaaag cttgtctaag ccgttctaag
     1981 acaattcttg attaatttcc cttaatctag tcgagaagcg ccttacaggc ggtcagggca
     2041 agccctaaca accctaaaac aagtgatact taaaaacacc acacaaattt aattttttaa
     2101 gatataataa cttgtcgcct atccgacagt cttgcgactg gaaggaggtg tatctaatgt
     2161 ttgaactgtt catttgcccc cttcttgtag gagtttgtgt ctctttattc agttattggt
     2221 tgaataaccg aaacaagaag taaaatcaat aaaggcgaca tcaacctgaa aaaaagcccc
     2281 agagaatccg acctctctgg ggctttcgtg tatctaatgt ttgatcattt gcacattcag
     2341 tttaacatga ttttaaatca aattcaattt tgttgttgct gatctaacca ttccttgaca
     2401 ctttttagat agtttaaggc ggtttctttt tttgtcggat tttgcatgtt agttgcttgt
     2461 tgaagtaaga tttcatagcg ttcatatgtg acagattgtt gtttttcttt tttttcttct
     2521 tcactggcaa accaagggtt tgtgtctcca aatagatcac tacaggcttc ttctatcttt
     2581 gtatctttta catattgcac gtcttttttg gttcgctcat agttgtcttg aacctctttt
     2641 tgctgttttt ctggcaagtt ttgataaaga cgagtgagtt tttccatgtt tttatccgtc
     2701 aaacgttttt cttgattcac aaaaatatcg ttcatttcct cataacaagt ggtaaactct
     2761 tccatttttt gtgctaatgc ttgctctttt ttctttttat cacgcgctga ttcatacttt
     2821 tgagtgacat ttcctaataa aatacttcta gaaacgcgct tatttccctc taaaacgccg
     2881 aataagccgc ttgaaacgat tatcaaggaa aatatacagc caagaaagaa aaatcgctta
     2941 aatcgaattt tagagccttt taatgaatat cttgtttgaa aaattttata gatgagccaa
     3001 atacttcctg aaatactaac aaaaatgagt agatagggta aataaaatgc cattttagcc
     3061 tccccatact aaccaaaatg cgaacagtgt caggactaag caacataagg gggcaagaaa
     3121 atccaatgat cgttgaataa gcaccttttt ctgttcctta tttcgctggt ctaatagctc
     3181 ttgatggagt tttttaactt gtaaatcaat cgtagtttgt atcctttctt gcgtataagg
     3241 aacgagaaaa gcacgttgtt tctttatttc gttttctatg tcttttaacg ttttagagcg
     3301 ataattcttg ttggtggtct gttcctcttt caacgtgttg ttcatggttt cttcgagccg
     3361 gtctaatttc cggttgttca ggcgtaagag ttcgttgttc tcttcgtact tcttctgcag
     3421 tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgctgttgg ttataggttc
     3481 gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtagtccttt cctagtctgt
     3541 tggatcgtac ttttttcttt ttgtggatca attcgtaagt gaaatttttc ccgcgattga
     3601 agcaatgaat ccccaatgtt ttgagggaat tacaaaattg gtcataggtt ctggtttttt
     3661 tgtttttcat tgtttgatcg atcgcaatcc gaatttcgtc tttccaaaca ggttcatttt
     3721 tttcttttaa ttttatttca gacatggttc gtttttctgg tttctttcct cgctcagtga
     3781 ttggtaagtt atgtgcaaga caaatctcgt catttaggtc ataacatttg tttagaaatg
     3841 cttgaccatg agcttgaaat ttttttccag tctccagatt aacggagtta atgacaatgt
     3901 gattatgaat atggtctttg tctgtatgtg tgtaaatagc tatttggtga tcgggggcta
     3961 ttttttttgc atactctgcc cctagttgat tagctttttc agcggtaatt tctcctggtc
     4021 gaaaagattg aatcaataag tgtgcctgta cacggtcgtc ttttccatac agcatgcgaa
     4081 tttgtttcat ttcacttttg gcataatcga cgtcacagtt tattcctgtt ttaatggttg
     4141 ctcgtggttc tgcgtaatta attgcacgac tacaagaagt ggttcggctt accttaattg
     4201 ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata aacgttgagc
     4261 gtcaccagaa gcaacgtcat gttggtgtgt attgcaccac ttagcaattt gattgatatt
     4321 ccctccagct ttggctaaat cgcgcgcgat tgcctgggca atgtctggcg ccactttcgg
     4381 tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac tcatttgcaa
     4441 agcttcagcc gattgcttca gctttgcaaa ttcggattcg ctcactcgaa aactgatttg
     4501 ttttggctcg gatcgatagg tatatttttt agaagaattg ctagccaaat tttgattttg
     4561 ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg caataccatt
     4621 gtacctaaaa aggtacaatg agtaaagctc actaaaaggg tttggcaagc tctatatttg
     4681 ctagccaaat acctacgtat ttagtgcaaa taaccgcttg tcaaaggggc gcaagctccc
     4741 ccttgaatcc ccctcaaaag ccacgctcca caatacaaaa aaagctagca aatgctagcc
     4801 actttttgta aagtttcgct tatttcaccg aagatgaaat tgttgctttg caacaaaaaa
     4861 cataaatgct tttttgtgta aactactaac tatttagcta aaactgatag ttttattaaa
     4921 cattcagcaa tatttttagc caatttttta tcagactttc ggcgagtcat tatgataata
     4981 tgtccacttt ttgttacttc taccaaaaat ctagcaataa ctctcccatt cttcagttga
     5041 tttagtttca taagtctttt gtaattgacg gtttaatttt tttggcaatt gcaaagaata
     5101 aataagtcta tcataaaatt tttcatactc ttttttcaat aaaatcactt gggtgcctcc
     5161 tttttattaa gtgattattt aaattttggg ataggagaga tggaatataa aataaatacc
     5221 cgtggtgcta gttttaaatc agctagcatc acgggtaaaa attataattc tataatatta
     5281 gttccatagt ttagaacatt taagtcatgt gttactagta caacggcttt tttttctttt
     5341 tctgttaaat ttttcagaaa gttcattata aaagttgagt tttctttatc taaatttcct
     5401 gtaggttcat ctgcaaataa tatatcaaat ggttttaaca tatttctaat taatgctatt
     5461 ctttgttgtt ctccaccgct taagagaaag attttttctt ttaagatatc ttttggcaaa
     5521 gaaaaatctg ttaataaatt ataaatatga tcattggaaa tattactgtt aaactttttt
     5581 gctaattcga aattgtattg tactgtttga ttttcaagaa gagcatagtt ttgaaataaa
     5641 tagctaatat gatttcttcg tagacgttgt atatcatgtt tagaaacttt cttttcaaaa
     5701 tagaataagt taccggtata ttgtttatct attaggctta aattatttaa cagagtagtt
     5761 tttccactac cactttttcc tgttactata ataaaatctt tttctgatat tgaaaagttc
     5821 caattttgtt tgatcttttt tttattaatt gacagggtga aattctcaac tgataatatc
     5881 ataaaatatt ctccttaaaa tatttaaaac tatttttttt ataaataata atagaagtaa
     5941 ttagatttat taaaaagatc ataaaaatta taaaatacaa gtaagtgttt tttgaaaata
     6001 tcaattgaaa acacatagta gcaaataact ctattgtaaa agggagtaaa cataaatcta
     6061 aaacttgact tattggtgaa tatccaaata ttttttttaa agctattttt tttgaattta
     6121 tagtaaaact aacaaaaatc atttgaaaat taattattat aaataatcct aatattaaaa
     6181 gatattgtag atatgcattt aatttattta cttttttttg agataataat tcaccagttt
     6241 cttgaaaaac agaataagaa aaatttatga tattttctag gttaaattct tttatatacg
     6301 gattaatttg attaaaaaag ttttggtttg aagtatgttt ctgatcaaaa aataatccac
     6361 catcactagt caatatattt ccccaaaata atgattctgt tatttctgga ttaataactc
     6421 taactatggg atcagttatt gtatagttat cttttgcctt tccatataag gtattaaaac
     6481 taaaatactt ttggttatta ttaacaaata ttagttttat ttttaaattt ttcaaattag
     6541 gatttttata aggagcttga ggaggagagt cctcaagtct aaactgtatg tcttctaaat
     6601 aatttttttc aatcattttt gcatattctt tatatttttt aggtactaga atgtaagtag
     6661 ttagtggatc agagtttatt aaatttaagt cagtagattc ttcaaaagaa ataggattgt
     6721 aatattttag atagtttaaa tcaacacata ttcctgcacc agcaggttga ctaacatagt
     6781 cttcaaaatt tggataaact ttttgtccaa taaaaaattc tttattattc tcttggatag
     6841 ttgcgtaatt gtatggcgcg ataataaaag ttttataatt cttctgtatt ttataaacaa
     6901 atttactagc ttttttcaag tagttatttt cttctacggt gttgtttcta tttaattgat
     6961 tagtaatatt tgttttgtat aaattagtag ttctttccca atatttaaga gaattatttt
     7021 cggcttcatt atgggtattt attacttttg tagaagctat taacagaagg atcatgctta
     7081 agaatagaca gatatttaat atacaaagat ttaacttaag tggtcgtgaa ttttttagat
     7141 aattttttat actagataaa tgatagatac ttagagtaac tatgataaat aaaaaactaa
     7201 ttataaaatg aaaaatacta catagaagaa atgataggaa aaaattttcg ttactgtaat
     7261 atatgctcaa aaaaatagat aaaatgaaag ttataatcaa agaacaaaat aagaggttta
     7321 actctttaat agaatctttt atagtatcaa aagagttata gcctaacatt ttttttagag
     7381 tgatttcttt catttttgaa aaagcaaata taattgtagt aattacaata aatatactaa
     7441 gatagcagat aacaagaaca atattagtat taatttgaaa agtatctgat tgattgatat
     7501 tggaaatagt aggtgttcca tattttttca gtacaggaat taatttgttt gagtttcctt
     7561 gtacatataa tatttcactt aatccaatat tttccacatg agaaaaagga taaatattta
     7621 tatttaaaaa attatttgga agttttaatt ggtacttaga atttttgtta ttagtataat
     7681 ggactttaga atcgtttctt gctaatatat tttttttaga aaatttccag tgtttgttag
     7741 cgttagggtt tgtagcaaga atagttagtt ggttaacagc agtaaaacta tattgagaaa
     7801 tattaatatt ttcttgtgta gatatttcat acaaagcgtt tatataactg ttaaagtcca
     7861 tatcatcttt atattgcata tgaatttcag cggtattttt atatagcgct tcagaagtct
     7921 ttataccatt tataacatta gtagaaaaaa atagtattgt aatgcagaca aaaagtgata
     7981 aaacagcgtt tatttttttt aacatatttt gaatttcctt tcaaatgtaa ataaaaaaga
     8041 gagtttaaac tctctttttt aataaacatt ccaccaacca gtatttccag taagtgtggc
     8101 attgcatgtt gcataagctc ttactccagc tttctgaact gatcgtactt gaccgcctcg
     8161 tccattttta gctgttgcac tatgtttagc tctagcatgg tagtaatttg cataaacttt
     8221 aaatccatta accccacagt tgaaatcccc gccgccagca gcatatctca atttactaga
     8281 agaaaatcct tcttctgtag cttccattcc tcctccgtaa atggcggtat ggtcttcggg
     8341 tacatctaga tttaaagctt ctgtggcact agctattcct ccactagtta ataagacacc
     8401 cattaaagca gaaagacata aaaatttttt cataacttat ttctcctatc ttttttacta
     8461 gaattatttt attttagtgt gttttataaa ataaagaact tttttggcgc gttagtattt
     8521 ttatgataaa attttatgta aataatcgtt tacataggag aaaaaatgga ggatttagga
     8581 tctaagctaa gagaattaag agtgaaaaaa gggtatactc aagaatatgt ggcaagaaaa
     8641 tttaacgtta cacgtcaaac tatttcaaag tgggaaaata acaaaacaac accagatata
     8701 gagacactaa aaaaaatttg tgaattttat agtactaaca taaattctat tttaaatgaa
     8761 gataattcaa aaaataaaaa aaataattta ctggtattga cagctattat ttttaatata
     8821 tttgttacta tattaggttt atttgaattg attcaaatga atttttttag ctttacacat
     8881 ggaatattta cttggtttgc tctatctgga ttaatagtaa tagatatctt aaattatttt
     8941 tatatttact attcttattt aaaaaatgaa aaattttttt atacaatgtt tttcccccta
     9001 gttattttga ttattttatt aagctaatat gaagtagtta gttatttaat tgcatagttg
     9061 acataatgta cactatgcca agtagcccct ctaccaatgg atagagacgc tcactttgtg
     9121 atttttaatt ggtctagcta aaagcaactt tctaaagctt gatttttcgc ggttatctat
     9181 atctaattct ttgtgcattt ttatctgtca tttttttaag acttgttatt ttttgtaaaa
     9241 tatggtaaat ttttggtagc gcttcataag cgcgtgcttt ttcaagaata aacactcatt
     9301 attttttaac tggctgc
//
