LOCUS       NZ_LR135253             2189 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6988 plasmid 11.
ACCESSION   NZ_LR135253
VERSION     NZ_LR135253.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884873
            Assembly: GCF_900639415.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:25:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,264
            CDSs (total)                      :: 3,173
            Genes (coding)                    :: 3,043
            CDSs (with protein)               :: 3,043
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 55 of 130
            Pseudo Genes (incomplete)         :: 79 of 130
            Pseudo Genes (internal stop)      :: 29 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135253.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6988"
                     /db_xref="taxon:1352"
                     /plasmid="11"
     gene            complement(<252..434)
                     /locus_tag="EQB64_RS16980"
                     /pseudo=""
     CDS             complement(<252..434)
                     /locus_tag="EQB64_RS16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313295.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(893..1858)
                     /locus_tag="EQB64_RS16990"
     CDS             complement(893..1858)
                     /locus_tag="EQB64_RS16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002305972.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVGK
                     LKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKYQE
                     GQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQLRRG
                     KATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYKYRNLL
                     KKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          complement(897..1780)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.588"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 tcagctatcg gctttcacaa tctaacacga tcgttccgct accagtcgcc ttacaggctc
       61 cattttcgct cctctaacgt gttttaacga ttgtgccgat aactaaacga aataaacgct
      121 aaaaccgctc agaacgcaaa atatgccatt ttagagctat tgtagaaaat gtagtttgaa
      181 atgcaatttt cttctttcag ctttttattt cccacttgta aatctgctaa ttattttcta
      241 ccagacaaac cattgatttt ttggctatca gttaactcac gattattatg agaaatgctt
      301 gtattctttg ttcccttttt gaacgaaata ctaaacccta aggtgaaccc ctccttcgcg
      361 ctgtcgctaa taaaaagagt gtaaaccttt ctgttactct ttgacaaaga gtaacatttt
      421 cctaattgtg acatggctgt cacaattcca aacgctctcc gagggttttc gctgtcacaa
      481 gtgacaacga aaaaaggagc cgaaagcgtg tcacgctctc aactcctttt acgctttatt
      541 ttgcttcacg caaaataatc gcttatctta aacattgacg taaatttact ctcacttatc
      601 tgactttttt actcggtatc actccgagta agccgtcata ttcattggga taatctcgtc
      661 tgcttcgctc taattttcca gagtgagggg taactccttc ccccgaatta ccccaaagag
      721 tgacactggg atatccctaa atttattata aaaagccaag tctctttgcg caactgtttt
      781 atccaagtcg gcatttgcgg attcgttagc gttttttcgc tcttgctgca aacgcacgac
      841 ccaccatgcc gacttgtgct gaaaataaaa aagtggacaa aaattcatcc actcattctt
      901 ccgtcacgtc caaccccaaa agccacgtat ccaattcttt tttcaaaaca tctgccgatt
      961 ccgaaaatgt ctctcgtaaa tgattaacta actcatcact ttggctttct tttttcaaca
     1021 aatttcgata tttatatttt gtatgacgag ccaagccagc ccataaattt tgatctgaaa
     1081 tcaatcctgc aatcataatt ctatcagtat gctttgtatc agtcggcaaa aaatgaggac
     1141 ttgcgaaact gtctaaactt tcatgaacca tcgcatacca atcagtagct tttccacgcc
     1201 gtaactgcaa ttccagtcgc caccaagttt ttatttccgg tggtacaatt ttccgtttgg
     1261 tttcttgttc caattttttg ttgtacatcc ttatttgtcg ctcactggca cgactacccc
     1321 aatatgccgt ttccagcttc ccagaccgcc catagattgg tttgaatgaa acagggtcaa
     1381 ccacacgata ttgcgtaata aaatcatcag gcacatcgat aatgtcgcaa gcaatatcag
     1441 cacgtgagaa atgtggttct aaaaataaat catggataaa gttcttcatc gaacccgcca
     1501 aaaattggct aattttattt gggttaaagt caattcgccc ttgcccttct tgatatttca
     1561 aaatttcaat gtaagcaata ttctcatcat gcttatccac aatttgccaa ccatttagac
     1621 caacagattt aacataacca ttattttcat tcagtcgcat taactgttca aagtctaaaa
     1681 tcaatacatc attttgcgtg tgataataaa tattctcttt gagttttcca acaatggtaa
     1741 ttcggtcaat tgaccactgt attttaaggg gggtggtcaa agtttgcaca cttaaccgcc
     1801 tgttagatag ggctgttaaa ttttcccact catttatgat cgtgccacgt ttactcaaat
     1861 tttgttcctc ctcaaagaaa aagagacttt tggaaagaat ttccagagtt cacgtaaccc
     1921 taaaaattca tatccaaaaa tctctagctt gaagaaacct aaaaatattc gtataatgaa
     1981 cctgagttac ggagctaggt gaagtagttt ccgtaaatag tcgagagtgt tagcgcactg
     2041 ctcggctttt attttgctct tttttctcgt tttctttgat tatagcactc ctcctaaaaa
     2101 aataaaaaga cctcagggaa tttcattccc cgaacccctt gagcctcctc ctgccgaaat
     2161 ggaaagggcg cacccgctgt ttatttcat
//
