LOCUS       NZ_LR135252             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6988 plasmid 10.
ACCESSION   NZ_LR135252
VERSION     NZ_LR135252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884873
            Assembly: GCF_900639415.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:25:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,264
            CDSs (total)                      :: 3,173
            Genes (coding)                    :: 3,043
            CDSs (with protein)               :: 3,043
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 55 of 130
            Pseudo Genes (incomplete)         :: 79 of 130
            Pseudo Genes (internal stop)      :: 29 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6988"
                     /db_xref="taxon:1352"
                     /plasmid="10"
     gene            <1..266
                     /locus_tag="EQB64_RS16960"
                     /pseudo=""
     CDS             <1..266
                     /locus_tag="EQB64_RS16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(603..743)
                     /locus_tag="EQB64_RS16965"
     CDS             complement(603..743)
                     /locus_tag="EQB64_RS16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(740..1714)
                     /locus_tag="EQB64_RS16970"
     CDS             complement(740..1714)
                     /locus_tag="EQB64_RS16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2514..2795
                     /locus_tag="EQB64_RS16975"
     CDS             2514..2795
                     /locus_tag="EQB64_RS16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          complement(744..1690)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgactaccag caaccaagcc aaactaaatg ctatgggaaa agaaatttcc atcatgtccg
       61 aaatcattgc agaatttgct gatttatatc taaaagatac aggtattctt attggcacaa
      121 attctaaagt gtatcaagaa acgaaagaaa aaattgaaaa acaacttaat ccctccaaac
      181 gaaatcaacc aaaaaatcgt caaaaagaaa ccaatcaaac atttacttct aaaaatttcg
      241 acccaacaaa ttttctaaaa agataaaaaa taaaccagga gatttaaatt cctggtttat
      301 tttttgggta ggatcaccat cttaatagca acatttacaa taagatagta accaattgaa
      361 tgaaaaaaac gaatttcaga agaaataaca gaaaaaaaga taaaaacaaa aaaattataa
      421 atacatgatt tgaattcata atcgtaaaaa aagctgctcg tacgagcagc taaataagaa
      481 aaagattggt atgttttaag gataaaataa gtataacaaa tttttcacat agagcaaagg
      541 gggcgaaaat cgcccccttt ttttcgtttt aaaaccccaa aacccaaaga cataaatttc
      601 ggtcaattga ccactgcatt tttttcttta tactgagcaa ggtcaaatcc cgaattgacc
      661 agttgattgc gcaccgtatt tttcgataca cccaattcat cagcaagttt ttttagggtc
      721 attttttcta tcgaatcact cattcagcac ctacttccgt cacatcaagt ccttgaagcc
      781 atgtatctaa ttctcgtttc aaatcatctg ccgactccga aaatgtttct cgcaaatgat
      841 tcgtcaactc atcattttga ctttcttgct ttaacaattc acgatattta tattttgaat
      901 ttctatgaat ttgcccccag taactatgtt ccgttataag agcagtaatc atcattctgt
      961 cagttacttt cacatcttct ggcaaatagt gaggacttgc aaagctatct aaactttcat
     1021 gaaccattgc atgccaatca gttgctttcc cccttcgcaa ttgcaattct agtcgccacc
     1081 aagtatcgat ttctttcgga acaactttcc gttttctttc ttgttccaat tttttgttat
     1141 acatccgaat ctgtcgttca ctagcacgac ttccccaata tgctgtttcc agtttcccag
     1201 accgaccgta aattggtttg aaagaaacag gatcaaccac ccgatattgg gtaatgaaat
     1261 catctggcac gtcaataatg tcacaagcaa tatctgcacg tgagaaatgt ggttctaaaa
     1321 ataaatcatg gataaagttc ttcatcgagc ccgccagaaa ttggctaatt ttattggggt
     1381 taaagtcaat ccgccctttt ccctcttgaa acttaagaat ctcaatataa gctatatttt
     1441 cctcatgttg gtctagtaat tgccagccat tatttccaac cgcttttaaa tatccgttac
     1501 cttcgtttag acgcataagc tgttcaaaat ctaaaatcaa cacatcatgg ggagtgtgat
     1561 aataaatatt ttcccttaat gtaccaacaa tggtaattcg gtcaattgac cattgcattt
     1621 ttaagggggt gcacatagtt tgtgcattta accgcctgtt agatatgcgg ttaagttttt
     1681 cccactcatt tatgatcgtg ccacgtttac tcaaattttg ttcctcctca aagaaaaaag
     1741 agatttttgg aaagaatttc cagagttcac gtaactctaa aaatccatat ccaaaaatct
     1801 ctagcttgaa gaaacctaaa aatattcgta taatgaattt gagttacgga actaggtgag
     1861 tagtttccgt aattagccga agggtgcttc caacaccgtt cggcttttat tttgctcttt
     1921 tttctcgttt tctttgatta tagcactcct cttaaaaaaa taaaaagacc tcagggaatt
     1981 tcattccccg aaccccttga gcctcctcct gccgaaatta tttcacgctt ccgctcttca
     2041 tttcattcag ttctcggctt gcacaatcta acacgccgtt tcgctaccag tcgccttaca
     2101 ggctccattc tcgcttctct tacgtgtttt aacgattgtg ccgataacta aacgaaatga
     2161 acgctaaaac gtcttagaac gcaaaatacg cgcttataaa atcatttcat cttgattaca
     2221 tgcccgtcat aatcgttcag tttagtttca ttttcgtaaa ctttttttat ttttacgaac
     2281 agaaaaaagt cgcacatcac cattttataa agagcataag gaaacgtgta cccactttct
     2341 aaaaaaagtg ggtagatagg ctgtgcctat cacgtttctc agaaaaaaat ttgatatcac
     2401 tttttcgcca tcgtggctag cttatgtata gtattttagc aaaaaacagc tatacaaata
     2461 ctatattcgt atgatataca taaattatac acaaatgcga aaggagtttt tctatggaca
     2521 ttttagttcg gaagatcgat tcaaaggtta ttgcaaaaat agacaaacgt tgccaagaac
     2581 tcacaaataa aactggcaaa aaatggagtc gaaatcgcta cttaaaagtc ctaatcgaaa
     2641 acgattttga ccacgcactt atgcattaca agaaagatca attcgattta ttactcgaaa
     2701 aatttatcgc tattcaagaa aaaaatactg aaacgctgaa tgaatatgtt cgtacaaaca
     2761 accaactcat tgaaacactt atcggaggaa gataacatga gaaaaacttc tgttcaaaaa
     2821 cacgttaccc tttatcccag tcacgtggac gttttagatt ccattatcga aagaaatcca
     2881 gatatcaaca attactcgga agccattcgt tttttatgta tgagttatga acctgatacc
     2941 aatacta
//
