LOCUS       NZ_LR135250             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6988 plasmid 8.
ACCESSION   NZ_LR135250
VERSION     NZ_LR135250.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884873
            Assembly: GCF_900639415.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:25:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,264
            CDSs (total)                      :: 3,173
            Genes (coding)                    :: 3,043
            CDSs (with protein)               :: 3,043
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 130
            CDSs (without protein)            :: 130
            Pseudo Genes (ambiguous residues) :: 0 of 130
            Pseudo Genes (frameshifted)       :: 55 of 130
            Pseudo Genes (incomplete)         :: 79 of 130
            Pseudo Genes (internal stop)      :: 29 of 130
            Pseudo Genes (multiple problems)  :: 26 of 130
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135250.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6988"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            <1..344
                     /locus_tag="EQB64_RS16875"
     CDS             <1..344
                     /locus_tag="EQB64_RS16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_127840774.1"
                     /translation="GVLLTSGGIASATEALNLDVPEDHTAIYGGGMEATEEGFSSSKLR
                     YAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGVRAYATCNATLT
                     GNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            398..2524
                     /locus_tag="EQB64_RS16880"
     CDS             398..2524
                     /locus_tag="EQB64_RS16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            2521..3141
                     /locus_tag="EQB64_RS16885"
     CDS             2521..3141
                     /locus_tag="EQB64_RS16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            3352..3513
                     /locus_tag="EQB64_RS17495"
     CDS             3352..3513
                     /locus_tag="EQB64_RS17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305245.1"
                     /translation="MKLNQLKNGRVIARFLVEVTKSGHIIIMTRRKSDKKLAKNIAECL
                     IKLSVLAK"
                     /name="hypothetical protein"
     gene            3834..4214
                     /gene="mobC"
                     /locus_tag="EQB64_RS16890"
     CDS             3834..4214
                     /gene="mobC"
                     /locus_tag="EQB64_RS16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            4196..5110
                     /locus_tag="EQB64_RS16895"
     CDS             4196..5110
                     /locus_tag="EQB64_RS16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5070..5348
                     /locus_tag="EQB64_RS17660"
     CDS             5070..5348
                     /locus_tag="EQB64_RS17660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            5350..6039
                     /locus_tag="EQB64_RS16905"
     CDS             5350..6039
                     /locus_tag="EQB64_RS16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6160..6246)
                     /locus_tag="EQB64_RS16910"
     CDS             complement(6160..6246)
                     /locus_tag="EQB64_RS16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            6891..7628
                     /locus_tag="EQB64_RS16915"
     CDS             6891..7628
                     /locus_tag="EQB64_RS16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            7656..8195
                     /locus_tag="EQB64_RS16920"
     CDS             7656..8195
                     /locus_tag="EQB64_RS16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            8211..8381
                     /locus_tag="EQB64_RS17230"
     CDS             8211..8381
                     /locus_tag="EQB64_RS17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(8693..9154)
                     /locus_tag="EQB64_RS16925"
     CDS             complement(8693..9154)
                     /locus_tag="EQB64_RS16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     BGC             1..5344
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6892..7628
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4197..5107
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgggtgtctt attaactagt ggaggaatag ctagtgccac agaagcttta aatctagatg
       61 tacccgaaga ccataccgcc atttacggag gaggaatgga agctacagaa gaaggatttt
      121 cttctagtaa attgagatat gctgctggcg gcggggattt caactgtggg gttaatggat
      181 ttaaagttta tgcaaattac taccatgcta gagctaaaca tagtgcaaca gctaaaaatg
      241 gacgaggcgg tcaagtacga tcagttcaga aagctggagt aagagcttat gcaacatgca
      301 atgccacact tactggaaat actggttggt ggaatgttta ttaaaaaaga gagtttaaac
      361 tctctttttt atttacattt gaaaggaaat tcaaaatatg ttaaaaaaaa taaacgctgt
      421 tttatcactt tttgtctgca ttacaatact atttttttct actaatgtta taaatggtat
      481 aaagacttct gaagcgctat ataaaaatac cgctgaaatt catatgcaat ataaagatga
      541 tatggacttt aacagttata taaacgcttt gtatgaaata tctacacaag aaaatattaa
      601 tatttctcaa tatagtttta ctgctgttaa ccaactaact attcttgcta caaaccctaa
      661 cgctaacaaa cactggaaat tttctaaaaa aaatatatta gcaagaaacg attctaaagt
      721 ccattatact aataacaaaa attctaagta ccaattaaaa cttccaaata attttttaaa
      781 tataaatatt tatccttttt ctcatgtgga aaatattgga ttaagtgaaa tattatatgt
      841 acaaggaaac tcaaacaaat taattcctgt actgaaaaaa tatggaacac ctactatttc
      901 caatatcaat caatcagata cttttcaaat taatactaat attgttcttg ttatctgcta
      961 tcttagtata tttattgtaa ttactacaat tatatttgct ttttcaaaaa tgaaagaaat
     1021 cactctaaaa aaaatgttag gctataactc ttttgatact ataaaagatt ctattaaaga
     1081 gttaaacctc ttattttgtt ctttgattat aactttcatt ttatctattt ttttgagcat
     1141 atattacagt aacgaaaatt ttttcctatc atttcttcta tgtagtattt ttcattttat
     1201 aattagtttt ttatttatca tagttactct aagtatctat catttatcta gtataaaaaa
     1261 ttatctaaaa aattcacgac cacttaagtt aaatctttgt atattaaata tctgtctatt
     1321 cttaagcatg atccttctgt taatagcttc tacaaaagta ataaataccc ataatgaagc
     1381 cgaaaataat tctcttaaat attgggaaag aactactaat ttatacaaaa caaatattac
     1441 taatcaatta aatagaaaca acaccgtaga agaaaataac tacttgaaaa aagctagtaa
     1501 atttgtttat aaaatacaga agaattataa aacttttatt atcgcgccat acaattacgc
     1561 aactatccaa gagaataata aagaattttt tattggacaa aaagtttatc caaattttga
     1621 agactatgtt agtcaacctg ctggtgcagg aatatgtgtt gatttaaact atctaaaata
     1681 ttacaatcct atttcttttg aagaatctac tgacttaaat ttaataaact ctgatccact
     1741 aactacttac attctagtac ctaaaaaata taaagaatat gcaaaaatga ttgaaaaaaa
     1801 ttatttagaa gacatacagt ttagacttga ggactctcct cctcaagctc cttataaaaa
     1861 tcctaatttg aaaaatttaa aaataaaact aatatttgtt aataataacc aaaagtattt
     1921 tagttttaat accttatatg gaaaggcaaa agataactat acaataactg atcccatagt
     1981 tagagttatt aatccagaaa taacagaatc attattttgg ggaaatatat tgactagtga
     2041 tggtggatta ttttttgatc agaaacatac ttcaaaccaa aactttttta atcaaattaa
     2101 tccgtatata aaagaattta acctagaaaa tatcataaat ttttcttatt ctgtttttca
     2161 agaaactggt gaattattat ctcaaaaaaa agtaaataaa ttaaatgcat atctacaata
     2221 tcttttaata ttaggattat ttataataat taattttcaa atgatttttg ttagttttac
     2281 tataaattca aaaaaaatag ctttaaaaaa aatatttgga tattcaccaa taagtcaagt
     2341 tttagattta tgtttactcc cttttacaat agagttattt gctactatgt gttttcaatt
     2401 gatattttca aaaaacactt acttgtattt tataattttt atgatctttt taataaatct
     2461 aattacttct attattattt ataaaaaaaa tagttttaaa tattttaagg agaatatttt
     2521 atgatattat cagttgagaa tttcaccctg tcaattaata aaaaaaagat caaacaaaat
     2581 tggaactttt caatatcaga aaaagatttt attatagtaa caggaaaaag tggtagtgga
     2641 aaaactactc tgttaaataa tttaagccta atagataaac aatataccgg taacttattc
     2701 tattttgaaa agaaagtttc taaacatgat atacaacgtc tacgaagaaa tcatattagc
     2761 tatttatttc aaaactatgc tcttcttgaa aatcaaacag tacaatacaa tttcgaatta
     2821 gcaaaaaagt ttaacagtaa tatttccaat gatcatattt ataatttatt aacagatttt
     2881 tctttgccaa aagatatctt aaaagaaaaa atctttctct taagcggtgg agaacaacaa
     2941 agaatagcat taattagaaa tatgttaaaa ccatttgata tattatttgc agatgaacct
     3001 acaggaaatt tagataaaga aaactcaact tttataatga actttctgaa aaatttaaca
     3061 gaaaaagaaa aaaaagccgt tgtactagta acacatgact taaatgttct aaactatgga
     3121 actaatatta tagaattata atttttaccc gtgatgctag ctgatttaaa actagcacca
     3181 cgggtattta ttttatattc catctctcct atcccaaaat ttaaataatc acttaataaa
     3241 aaggaggcac cctagtgatt ttattgaaaa aagagtatga aaaattttat gatagactta
     3301 tttattcttt gcaattgcca aaaaaattaa accgtcaatt acaaaagact tatgaaacta
     3361 aatcaactga agaatgggag agttattgct agatttttgg tagaagtaac aaaaagtgga
     3421 catattatca taatgactcg ccgaaagtct gataaaaaat tggctaaaaa tattgctgaa
     3481 tgtttaataa aactatcagt tttagctaaa tagttagtag tttacacaaa aaagcattta
     3541 tgttttttgt tgcaaagtaa caatttcatc ttcggtgaaa taagcgaaac tttacaaaaa
     3601 gtggctagca tttgctagct ttttttgtat tgtggagcgt ggcttttgag ggggattcaa
     3661 gggggagctt gcgccccttt gacaagcggt tatttgcact aaatacgtag gtatttggct
     3721 agcaaatata gagcttgcca aaccctttta gtgagcttta ctcattgtac ctttttaggt
     3781 acaatggtat tgcgaacgat acgtccgtag gacgtactga aagggtggga cttatgagcg
     3841 aacaaaatca aaatttggct agcaattctt ctaaaaaata tacctatcga tccgagccaa
     3901 aacaaatcag ttttcgagtg agcgaatccg aatttgcaaa gctgaagcaa tcggctgaag
     3961 ctttgcaaat gagtgtgccg gctttcgtga aagccaaggc acaaaacgcg cgtctggtga
     4021 caccgaaagt ggcgccagac attgcccagg caatcgcgcg cgatttagcc aaagctggag
     4081 ggaatatcaa tcaaattgct aagtggtgca atacacacca acatgacgtt gcttctggtg
     4141 acgctcaacg tttatccgaa aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc
     4201 aacaattaag gtaagccgaa ccacttcttg tagtcgtgca attaattacg cagaaccacg
     4261 agcaaccatt aaaacaggaa taaactgtga cgtcgattat gccaaaagtg aaatgaaaca
     4321 aattcgcatg ctgtatggaa aagacgaccg tgtacaggca cacttattga ttcaatcttt
     4381 tcgaccagga gaaattaccg ctgaaaaagc taatcaacta ggggcagagt atgcaaaaaa
     4441 aatagccccc gatcaccaaa cagctattta cacacataca gacaaagacc atattcataa
     4501 tcacattgtc attaactccg ttaatctgga gactggaaaa aaatttcaag ctcatggtca
     4561 agcatttcta aacaaatgtt atgacctaaa tgacgagatt tgtcttgcac ataacttacc
     4621 aatcactgag cgaggaaaga aaccagaaaa acgaaccatg tctgaaataa aattaaaaga
     4681 aaaaaatgaa cctgtttgga aagacgaaat tcggattgcg atcgatcaaa caatgaaaaa
     4741 caaaaaaacc agaacctatg accaattttg taattccctc aaaacattgg ggattcattg
     4801 cttcaatcgc gggaaaaatt tcacttacga attgatccac aaaaagaaaa aagtacgatc
     4861 caacagacta ggaaaggact acgaaaagga gacgatttta catgagctgg acagacgaga
     4921 acgaacctat aaccaacagc gaaccaacga gctacgaaga gctcgagcta gcattcgcga
     4981 aactgcagaa gaagtacgaa gagaacaacg aactcttacg cctgaacaac cggaaattag
     5041 accggctcga agaaaccatg aacaacacgt tgaaagagga acagaccacc aacaagaatt
     5101 atcgctctaa aacgttaaaa gacatagaaa acgaaataaa gaaacaacgt gcttttctcg
     5161 ttccttatac gcaagaaagg atacaaacta cgattgattt acaagttaaa aaactccatc
     5221 aagagctatt agaccagcga aataaggaac agaaaaaggt gcttattcaa cgatcattgg
     5281 attttcttgc ccccttatgt tgcttagtcc tgacactgtt cgcattttgg ttagtatggg
     5341 gaggctaaaa tggcatttta tttaccctat ctactcattt ttgttagtat ttcaggaagt
     5401 atttggctca tctataaaat ttttcaaaca agatattcat taaaaggctc taaaattcga
     5461 tttaagcgat ttttctttct tggctgtata ttttccttga taatcgtttc aagcggctta
     5521 ttcggcgttt tagagggaaa taagcgcgtt tctagaagta ttttattagg aaatgtcact
     5581 caaaagtatg aatcagcgcg tgataaaaag aaaaaagagc aagcattagc acaaaaaatg
     5641 gaagagttta ccacttgtta tgaggaaatg aacgatattt ttgtgaatca agaaaaacgt
     5701 ttgacggata aaaacatgga aaaactcact cgtctttatc aaaacttgcc agaaaaacag
     5761 caaaaagagg ttcaagacaa ctatgagcga accaaaaaag acgtgcaata tgtaaaagat
     5821 acaaagatag aagaagcctg tagtgatcta tttggagaca caaacccttg gtttgccagt
     5881 gaagaagaaa aaaaagaaaa acaacaatct gtcacatatg aacgctatga aatcttactt
     5941 caacaagcaa ctaacatgca aaatccgaca aaaaaagaaa ccgccttaaa ctatctaaaa
     6001 agtgtcaagg aatggttaga tcagcaacaa caaaattgaa tttgatttaa aatcatgtta
     6061 aactgaatgt gcaaatgatc aaacattaga tacacgaaag ccccagagag gtcggattct
     6121 ctggggcttt ttttcaggtt gatgtcgcct ttattgattt tacttcttgt ttcggttatt
     6181 caaccaataa ctgaataaag agacacaaac tcctacaaga agggggcaaa tgaacagttc
     6241 aaacattaga tacacctcct tccagtcgca agactgtcgg ataggcgaca agttattata
     6301 tcttaaaaaa ttaaatttgt gtggtgtttt taagtatcac ttgttttagg gttgttaggg
     6361 cttgccctga ccgcctgtaa ggcgcttctc gactagatta agggaaatta atcaagaatt
     6421 gtcttagaac ggcttagaca agctttcaga gtctcacacc tcctctttga acaacggttt
     6481 tagcaaagga gccggaggcg actgcgcagt ataatatgag agcatagcga tcacaccttt
     6541 ttagcgaccg tagggagcaa gttttccagt ttagaaaaat tcgcgaacct ttttggagga
     6601 aaaagtagga gttttttagg cgggaaattc aatgtgctct taagatatac tgttagatat
     6661 actgttagat atactgttaa taataactgt tagggattag caaccttatg ggagagtaag
     6721 tattgacata ggtagtgggg gataaattgt cacactatgg gggataaatt gtcacactat
     6781 gggggataaa ttgtcacact atgggggata aattgtcaca ctatggggga taaattgtgt
     6841 tttacatcct cttatgtcta tgataacata atttattgag gtgattttta ttgagtaacg
     6901 atatagttcg atataataat ggattaaatt ctgtcccttt aagaaaattt acccctatag
     6961 atatggattt attttgggga atatgttcta aaatgaaaag aaaaggtaca aaagaagaaa
     7021 tttttacatt taaacaaata aaagaattaa ttgattacaa tataaatgca cgtggcgacc
     7081 gttttgctaa tgatttaata aaaatgtcaa aaaaaatcat tactttgtct tttatttatg
     7141 aagacgaaaa aacatatgaa caattaaatc tgtttcaaaa atttaaaatc gataaagtga
     7201 atgaaacatt attaataaag gtttctgaag aatttgaata tatacttaat tcaatcggaa
     7261 ctaattttac tcgtttcgaa ttagaaaata tgacaaactt aaagagtagc tacgttaaag
     7321 agttatatcg ccaacttatg gctcataaag acagaagatc taaaaaaggt cactggtttg
     7381 taaaaataga agattttaga gctctattag caataccaga aagttatcgc atgagagata
     7441 ttgataaaca aatttttgcc caagcgaaaa aagaattttt aaacgttaat tcaactcacc
     7501 ctgctatttt taaaactttt gaaatcaaaa aaattaaagc ctacaaagga aataagatag
     7561 gatctctgca aatttatttt gaagaaaata taccttcaat taccttagat aattggttag
     7621 agaaataata cctaacaatt taggaggcaa ttatcatgca acccaaagaa acctttacaa
     7681 taaaggaatt atctgaatta tttgaaataa gtaggcaagc aatctctaaa catgttgata
     7741 aattagaccc gtccttatta gctaaaaatg agcgtggtta taaggttgta ttacgttctg
     7801 gagtattgca acttgctagc aacttaggta accaaaggtt actatatagg ttgcatgagt
     7861 atgaacaaga aaacgataaa aacgtttata tatcaagcaa aaacgtgatt gtaaacgagt
     7921 tgctagataa gttgcgtgaa gaaaatgaat ttttacgaga acaactttcc ctcaaagagc
     7981 aaaataattc taatatgcaa aaattactcg accagcaaca acagctcacc ctacaggcaa
     8041 atcaacagat cgaaaaactg caagaacagt tacaattagc ttatacggaa gaaactccag
     8101 aagatgaatc aactactatt ctttctgaag aaacagaatc gatagaaaaa cacacaaaaa
     8161 agaagaagca aaaatggtgg caactttgga aataactgga gggaacattc atgacaagaa
     8221 aaaatcttct attcggttct attttactag gaattctagg cttcagtccg atgttattgt
     8281 cagtaatcaa taaagggaat aaacatggtg aattgaaatt gaatccaaat tattacaaca
     8341 gtcctaacga agaattgttt gtaaaagaga cggataaata ataaaaaaga ctgctcttcc
     8401 gagcagccag ttaaaaaata atgagtgttt attcttgaaa aagcacgcgc ttatgaagcg
     8461 ctaccaaaaa tttaccatat tttacaaaaa ataacaagtc ttaaaaaaat gacagataaa
     8521 aatgcacaaa gaattagata tagataaccg cgaaaaatca agctttagaa agttgctttt
     8581 agctagacca attaaaaatc acaaagtgag cgtctctatc cattggtaga ggggctactt
     8641 ggcatagtcg acattatgtc aactatgcaa ttaaataact aactacttca tattagctta
     8701 ataaaataat caaaataact agggggaaaa acattgtata aaaaaatttt tcatttttta
     8761 aataagaata gtaaatataa aaataattta agatatctat tactattaat ccagatagag
     8821 caaaccaagt aaatattcca tgtgtaaagc taaaaaaatt catttgaatc aattcaaata
     8881 aacctaatat agtaacaaat atattaaaaa taatagctgt caataccagt aaattatttt
     8941 ttttattttt tgaattatct tcatttaaaa tagaatttat gttagtacta taaaattcac
     9001 aaattttttt tagtgtctct atatctggtg ttgttttgtt attttcccac tttgaaatag
     9061 tttgacgtgt aacgttaaat tttcttgcca catattcttg agtataccct tttttcactc
     9121 ttaattctct tagcttagat cctaaatcct ccattttttc tcctatgtaa acgattattt
     9181 acataaaatt ttatcataaa aatactaacg cgccaaaaaa gttctttatt ttataaaaca
     9241 cactaaaata aaataattct agtaaaaaag ataggagaaa taagttatga aaaaattttt
     9301 atgtctttct gctttaa
//
