LOCUS       NZ_LR135242             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7067 plasmid 8.
ACCESSION   NZ_LR135242
VERSION     NZ_LR135242.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884945
            Assembly: GCF_900639335.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:01:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 61 of 143
            Pseudo Genes (incomplete)         :: 82 of 143
            Pseudo Genes (internal stop)      :: 34 of 143
            Pseudo Genes (multiple problems)  :: 27 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135242.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7067"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            <1..903
                     /locus_tag="EQB63_RS16235"
     CDS             <1..903
                     /locus_tag="EQB63_RS16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_127836251.1"
                     /translation="QTMCTPLKMQWSIDRITIVGTLRENIYYHTPHDVLILDFEQLMRL
                     NEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQEGKGRIDFNPNKISQFLAGSMKNFI
                     HDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASER
                     QIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRGKATDWHAMVHESLDSFASPHYLPE
                     DVKVTDRMMITALITEHSYWGQIHRNSKYKYRELLKQESQNDELTNHLRETFSESADDL
                     KRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            900..1040
                     /locus_tag="EQB63_RS16240"
     CDS             900..1040
                     /locus_tag="EQB63_RS16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(1377..1793)
                     /locus_tag="EQB63_RS16245"
     CDS             complement(1377..1793)
                     /locus_tag="EQB63_RS16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1795..2076)
                     /locus_tag="EQB63_RS16250"
     CDS             complement(1795..2076)
                     /locus_tag="EQB63_RS16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347307.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     Typing          17..900
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.867"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 caaactatgt gcaccccctt aaaaatgcaa tggtcaattg accgaattac cattgttggt
       61 acattaaggg aaaatattta ttatcacact ccccatgatg tgttgatttt agattttgaa
      121 cagcttatgc gtctaaacga aggtaacgga tatttaaaag cggttggaaa taatggctgg
      181 caattactag accaacatga ggaaaatata gcttatattg agattcttaa gtttcaagag
      241 ggaaaagggc ggattgactt taaccccaat aaaattagcc aatttctggc gggctcgatg
      301 aagaacttta tccatgattt atttttagaa ccacatttct cacgtgcaga tattgcttgt
      361 gacattattg acgtgccaga tgatttcatt acccaatatc gggtggttga tcctgtttct
      421 ttcaaaccaa tttacggtcg gtctgggaaa ctggaaacag catattgggg aagtcgtgct
      481 agtgaacgac agattcggat gtataacaaa aaattggaac aagaaagaaa acggaaagtt
      541 gttccgaaag aaatcgatac ttggtggcga ctagaattgc aattgcgaag ggggaaagca
      601 actgattggc atgcaatggt tcatgaaagt ttagatagct ttgcaagtcc tcactatttg
      661 ccagaagatg tgaaagtaac tgacagaatg atgattactg ctcttataac ggaacatagt
      721 tactgggggc aaattcatag aaattcaaaa tataaatatc gtgaattgtt aaagcaagaa
      781 agtcaaaatg atgagttgac gaatcatttg cgagaaacat tttcggagtc ggcagatgat
      841 ttgaaacgag aattagatac atggcttcaa ggacttgatg tgacggaagt aggtgctgaa
      901 tgagtgattc gatagaaaaa atgaccctaa aaaaacttgc tgatgaattg ggtgtatcga
      961 aaaatacggt gcgcaatcaa ctggtcaatt cgggatttga ccttgctcag tataaagaaa
     1021 aaaatgcagt ggtcaattga ccgaaattta tgtctttggg ttttggggtt ttaaaacgaa
     1081 aaaaaggggg cgattttcgc cccctttgct ctatgtgaaa aatttgttat acttatttta
     1141 tccttaaaac ataccaatct ttttcttatt tagctgctcg tacgagcagc tttttttacg
     1201 attatgaatt caaatcatgt atttataatt tttttgtttt tatctttttt tctgttattt
     1261 cttctgaaat tcgttttttt cattcaattg gttactatct tattgtaaat gttgctatta
     1321 agatggtgat cctacccaaa aaataaacca ggaatttaaa tctcctggtt tattttttat
     1381 ctttttagaa aatttgttgg gtcgaaattt ttagaagtaa atgtttgatt ggtttctttt
     1441 tgacgatttt ttggttgatt tcgtttggag ggattaagtt gtttttcaat tttttctttc
     1501 gtttcttgat acactttaga atttgtgcca ataagaatac ctgtatcttt tagatataaa
     1561 tcagcaaatt ctgcaatgat ttcggacatg atggaaattt cttttcccat agcatttagt
     1621 ttggcttggt tgctggtagt cgtagtattg gtatcaggtt cataactcat acataaaaaa
     1681 cgaatggctt ccgagtaatt gttgatatct ggatttcttt cgataatgga atctaaaacg
     1741 tccacgtgac tgggataaag ggtaacgtgt ttttgaacag aagtttttct catgttatct
     1801 tcctccgata agtgtttcaa tgagttggtt gtttgtacga acatattcat tcagcgtttc
     1861 agtatttttt tcttgaatag cgataaattt ttcgagtaat aaatcgaatt gatctttctt
     1921 gtaatgcata agtgcgtggt caaaatcgtt ttcgattagg acttttaagt agcgatttcg
     1981 actccatttt ttgccagttt tatttgtgag ttcttggcaa cgtttgtcta tttttgcaat
     2041 aacctttgaa tcgatcttcc gaactaaaat gtccatagaa aaactccttt cgcatttgtg
     2101 tataatttat gtatatcata cgaatatagt atttgtatag ctgttttttg ctaaaatact
     2161 atacataagc tagccacgat ggcgaaaaag tgatatcaaa tttttttctg agaaacgtga
     2221 taggcacagc ctatctaccc acttttttta gaaagtgggt acacgtttcc ttatgctctt
     2281 tataaaatgg tgatgtgcga cttttttctg ttcgtaaaaa taaaaaaagt ttacgaaaat
     2341 gaaactaaac tgaacgatta tgacgggcat gtaatcaaga tgaaatgatt ttataagcgc
     2401 gtattttgcg ttctaagacg ttttagcgtt catttcgttt agttatcggc acaatcgtta
     2461 aaacacgtaa gagaagcgag aatggagcct gtaaggcgac tggtagcgaa acggcgtgtt
     2521 agattgtgca agccgagaac tgaatgaaat gaagagcgga agcgtgaaat aatttcggca
     2581 ggaggaggct caaggggttc ggggaatgaa attccctgag gtctttttat ttttttaaga
     2641 ggagtgctat aatcaaagaa aacgagaaaa aagagcaaaa taaaagccga acggtgttgg
     2701 aagcaccctt cggctaatta cggaaactac tcacctagtt ccgtaactca aattcattat
     2761 acgaatattt ttaggtttct tcaagctaga gatttttgga tatggatttt tagagttacg
     2821 tgaactctgg aaattctttc caaaaatctc ttttttcttt gaggaggaac aaaatttgag
     2881 taaacgtggc acgatcataa atgagtggga aaaacttaac cgcatatcta acaggcggtt
     2941 aaatgca
//
