LOCUS       NZ_LR135241             9507 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7067 plasmid 7.
ACCESSION   NZ_LR135241
VERSION     NZ_LR135241.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884945
            Assembly: GCF_900639335.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:01:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 61 of 143
            Pseudo Genes (incomplete)         :: 82 of 143
            Pseudo Genes (internal stop)      :: 34 of 143
            Pseudo Genes (multiple problems)  :: 27 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135241.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7067"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            1..945
                     /locus_tag="EQB63_RS16175"
     CDS             1..945
                     /locus_tag="EQB63_RS16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002303559.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="replication initiation protein"
     gene            966..1502
                     /locus_tag="EQB63_RS16180"
     CDS             966..1502
                     /locus_tag="EQB63_RS16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002303560.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            1518..1688
                     /locus_tag="EQB63_RS16465"
     CDS             1518..1688
                     /locus_tag="EQB63_RS16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2001..2462)
                     /locus_tag="EQB63_RS16185"
     CDS             complement(2001..2462)
                     /locus_tag="EQB63_RS16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000389746.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNLKNKQNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTIFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2595..2969
                     /locus_tag="EQB63_RS16190"
     CDS             2595..2969
                     /locus_tag="EQB63_RS16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000713874.1"
                     /translation="MKKFLCLSALMGVLLTSGGIVSATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            3023..5149
                     /locus_tag="EQB63_RS16195"
     CDS             3023..5149
                     /locus_tag="EQB63_RS16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002289343.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINIHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKTPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            5146..5766
                     /locus_tag="EQB63_RS16200"
     CDS             5146..5766
                     /locus_tag="EQB63_RS16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            5880..6017
                     /locus_tag="EQB63_RS16470"
     CDS             5880..6017
                     /locus_tag="EQB63_RS16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            6459..6839
                     /locus_tag="EQB63_RS16205"
     CDS             6459..6839
                     /locus_tag="EQB63_RS16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_002292464.1"
                     /translation="MSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6821..7735
                     /locus_tag="EQB63_RS16210"
     CDS             6821..7735
                     /locus_tag="EQB63_RS16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7695..7973
                     /locus_tag="EQB63_RS16865"
     CDS             7695..7973
                     /locus_tag="EQB63_RS16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            7975..8664
                     /locus_tag="EQB63_RS16220"
     CDS             7975..8664
                     /locus_tag="EQB63_RS16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303565.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     BGC             1..7969
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..945
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6822..7732
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaatg agattgtaaa ataccaccat gaattaaata caattccttt aagaaaattt
       61 acaccggttg aaatgaattt gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat
      121 aagacagttc gttttacgtt tgaccaacta aaagatttaa gtaactacaa agcaactgca
      181 aatgtacgat ttgtagaaga tttaaaagca acttatgaaa aaatcttatc tttacgcttt
      241 ggtcgtagaa gtgagtctgg gctttcttac gatatgtttg taatgtttac agaatttaaa
      301 atcaacgggg acgtagaaaa tccttacgta gatatacgaa tttacgaaaa agcactgcct
      361 ttgttaaata atttagacga gtgggtacgc tattctttac aacaattcaa cgagctacaa
      421 agtggctatg caaagactat gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca
      481 tatttctcca aagaagactt tcttgaatta ttagatattc ctaaaagtta taagcaacct
      541 gatatagata aacgagttat aaaacctata cgtcaagaac taactgcaat cttcaaagga
      601 ctaacaatca aaaagaaata cggaaaagga cggggaaaac ctgttattgg ctatcaattt
      661 acgttcaaac ctgagatcaa aaaagcagac gactttaaca aacatgcacc aaccaaagaa
      721 gaccaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga acccgctaca
      781 acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca acaaatcaaa
      841 caactagaaa aagagaaaaa agctcttgaa aaacaaacag agtccgcact ttcggaaaaa
      901 gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc taggaggcaa
      961 ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagatctt tttaagatga
     1021 gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata gctaaaaatg
     1081 agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctagc aacttaggta
     1141 accaaaggtt actagatagg ttgcatgagt atgaaaaaga caacgataaa aacatttata
     1201 tatcaagcaa aaacgtggtt gtaaacgagt tgctagataa gttgcgtgaa gaaaatgaat
     1261 ttttacgaga acaactttcc cttaaagagc aaaataattc taatatgcaa aaattactcg
     1321 accagcaaca acagctcacc ctacaggcaa atcaacagat cgaaaaactg caagaacagt
     1381 tacaattagt ttatacggaa gaaactccag aagatgaatc aactactatt ctttctgaag
     1441 aaacagaatc gatagaaaaa cacacaaaga agaagcaaaa atggtggcaa ctttggaaat
     1501 aactggaggg aatattcatg acaagaaaaa atcttctatt cggttctatt ttactaggaa
     1561 ttctaggctt cagtccgatg ttattgtcag tgatcaataa agggaataaa catggtgaat
     1621 tgaaattgaa tccaaattat tacaacagtc ctaacgaaga attgtttgta aaagaaacgg
     1681 ataaataata aaaaagactg ctctttcgag cagccagtta aaaaataatg agtgtttatt
     1741 cttgaaaaag caacgcgctt atgaagcgct accaaaaatt tatcatattt tatgaaaaat
     1801 aacaccccag taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaacggca
     1861 gaaaatcaag ccttaaaaag agcttttgac ctagaccaat taaaaatcac aaagtgagcc
     1921 cctgtatccc ttggtagaga ggctacttgg catagtcgac attatgtcaa ctatgcaatt
     1981 aaataactaa ctacttcata ttagcttaat aaaataatca aaatcactag gggaaaaaat
     2041 attgtataaa aaaatttttc attttttaaa taagaatagt aaatataaaa ataatttaag
     2101 atatctatta ctattaatcc agatagagca aaccaagtaa atattccgtg tgtaaagcta
     2161 aaaaaattca tttgaatcaa ttcaaataaa cctaatatag taacaaatat attaaaaata
     2221 atagctgtca ataccagtaa attattttgt ttatttttta aattatcttc atttaaaata
     2281 gaatttatgt tagtactata aaattcacaa atttttttta gtgtctctat atctggtgtt
     2341 gttttgttat tttcccactt tgaaatagtt tgacgtgtaa cattaaattt tcttgccaca
     2401 tattcttgag tatacccttt tttcactctt aattctctta gcttagatcc taaatcctcc
     2461 attttttctc ctatgtaaac gattatttac ataaaatttt atcataaaaa tactaacgcg
     2521 ccaaaaaagt tctttatttt ataaaacaca ctaaaataaa ataattctag taaaaaagat
     2581 aggagaaata agttatgaaa aaatttttat gtctttctgc tttaatgggt gtcttattaa
     2641 ctagtggagg aatagttagt gctacagaag ctttaaatct agatgtaccc gaagaccata
     2701 ccgccattta cggaggagga atggaagcta cagaagaagg attttcttct agtaaattga
     2761 gatatgctgc tggcggcgga gatttcaact gtggggttaa tggatttaaa gtttatgcaa
     2821 attactacca tgctagagct aaacatagtg caacagctaa aaatggacga ggcggtcaag
     2881 tacgatcagt tcagaaagct ggagtaagag cttatgcaac atgcaatgcc acacttactg
     2941 gaaatactgg ttggtggaat gtttattaaa aaagagagtt taaactctct tttttattta
     3001 catttgaaag gaaattcaaa atatgttaaa aaaaataaac gctgttttat cactttttgt
     3061 ctgcattaca atactattct tttctactaa tgttataaat ggtataaaga cttctgaagc
     3121 gctatataaa aataccgctg aaattcatat gcaatataaa gatgatatgg actttaacag
     3181 ttatataaac gctttgtatg aaatatctac acaagaaaat attaatattt ctcaatatag
     3241 ttttactgct gttaaccaac taactattct tgctacaaac cctaacgcta acaaacactg
     3301 gaaattttct aaaaaaaata tattagcaag aaacgattct aaagtccatt atactaataa
     3361 caaaaattct aagtaccaat taaaacttcc aaataatttt ttaaatataa atatttatcc
     3421 tttttctcat gtggaaaata ttggattaag tgaaatatta tatgtacaag gaaactcaaa
     3481 caaattaatt cctgtactga aaaaatatgg aacacctact atttccaata tcaatcaatc
     3541 agatactttt caaattaata ctaatattgt tcttgttatc tgctatctta gtatatttat
     3601 tgtaattact acaattatat ttgctttttc aaaaatgaaa gaaatcactc taaaaaaaat
     3661 gttaggctat aactcttttg atactataaa agattctatt aaagagttaa acctcttatt
     3721 ttgttctttg attataactt tcattttatc tatttttttg agcatatatt acagtaacga
     3781 aaattttttc ctatcatttc ttctatgtag tatttttcat tttataatta gttttttatt
     3841 tatcatagtt actctaagta tctatcattt atctagtata aaaaattatc taaaaaattc
     3901 acgaccactt aagttaaatc tttgtatatt aaatatctgt ctattcttaa gcatgatcct
     3961 tctgttaata gcttctacaa aagtaataaa tatccataat gaagccgaaa ataattctct
     4021 taaatattgg gaaagaacta ctaatttata taaaacaaat attactaatc aattaaatag
     4081 aaacaacacc gtagaagaaa ataactactt gaaaaaagct agtaaatttg tttataaaat
     4141 acagaagaat tataaaactt ttattatcgc gccatacaat tacgcaacta tccaagaaaa
     4201 taataaagaa ttttttattg gacaaaaagt ttatccaaat tttgaagact atgttagtca
     4261 acctgctggt gcaggaatat gtgttgattt aaactatcta aaatattaca atcctatttc
     4321 ttttgaagaa tctactgact taaatttaat aaactctgat ccactaacta cttacattct
     4381 agtacctaaa aaatataaag aatatgcaaa aatgattgaa aaaaattatt tagaagacat
     4441 acagtttaga cttgaagact ctcctcctca agctccttat aaaactccta atttgaaaaa
     4501 tttaaaaata aaactaatat ttgttaataa taaccaaaag tattttagtt ttaatacctt
     4561 atatggaaag gcaaaagata actatacaat aactgatccc atagttagag ttattaatcc
     4621 agaaataaca gaatcattat tttggggaaa tatattgact agtgatggtg gattattttt
     4681 tgatcagaaa catacttcaa accaaaactt ttttaatcaa attaatccgt atataaaaga
     4741 atttaaccta gaaaatatca taaatttttc ttattctgtt tttcaagaaa ctggtgaatt
     4801 attatctcaa aaaaaagtaa ataaattaaa tgcatatcta caatatcttt taatattagg
     4861 attatttata ataattaatt ttcaaatgat ttttgttagt tttactataa attcaaaaaa
     4921 aatagcttta aaaaaaatat ttggatattc accaataagt caagttttag atttatgttt
     4981 actccctttt acaatagagt tatttgctac tatgtgtttt caattgatat tttcaaaaaa
     5041 cacttacttg tattttataa tttttatgat ctttttaata aatctaatta cttctattat
     5101 tatttataaa aaaaatagtt ttaaatattt taaggagaat attttatgat attatcagtt
     5161 gagaatttca ccctgtcaat taataaaaaa aagatcaaac aaaattggaa cttttcaata
     5221 tcagaaaaag attttattat agtaacagga aaaagtggta gtggaaaaac tactctgtta
     5281 aataatttaa gcctaataga taaacaatat accggtaact tattctattt tgaaaagaaa
     5341 gtttctaaac atgatataca acgtctacga agaaatcata ttagctattt atttcaaaac
     5401 tatgctcttc ttgaaaatca aacagtacaa tacaatttcg aattagcaaa aaagtttaac
     5461 agtaatattt ccaatgatca tatttataat ttattaacag atttttcttt gccaaaagat
     5521 atcttaaaag aaaaaatctt tctcttaagc ggtggagaac aacaaagaat agcattaatt
     5581 agaaatatgt taaaaccatt tgatatatta tttgcagatg aacctacagg aaatttagat
     5641 aaagaaaact caacttttat aatgaacttt ctgaaaaatt taacagaaaa agaaaaaaaa
     5701 gccgttgtac tagtaacaca tgacttaaat gttctaaact atggaactaa tattatagaa
     5761 ttataatttt tacccgtgat gctagctgat ttaaaactag caccacgggt atttatttta
     5821 tattccatct ctcctatccc aaaatttaaa taatcactta ataaaaagga ggcacccaag
     5881 tgattttatt gaaaaaagag tatgaaaaat tttatgatag acttatttat tctttgcaat
     5941 tgcccaaaaa attaaaccgt caattacaaa agacttatga aactaaatca actgaagaat
     6001 gggagagtta ttgctagatt tttggtagaa gtaacaaaaa gtggacatat tatcataatg
     6061 actcgccgaa agtctgataa aaaattggct aaaaatattg ctgaatgttt aataaaacta
     6121 tcagttttag ctaaatagtt agtagtttac acaaaaaagc atttatgttt tttgttgcaa
     6181 agcaacaatt tcatcttcgg tgaaataagc gaaactttac aaaaagtggc tagcatttgc
     6241 tagctttttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc
     6301 cctttgacaa gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct
     6361 tgccaaaccc ttttagtgag ctttactcat tgtacctttt taggtataat ggtattgcga
     6421 acgatacgtc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa aatcaaaatt
     6481 tggctagcga tccttctaaa aaatatacct atcgatccga gccaaaacaa atcagttttc
     6541 gagtgagcga atccgaattt gcaaagctga agcagtcagc tgaagctttg caaatgagtg
     6601 tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct ggtgacaccg aaagtggcgc
     6661 cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat atcaatcaaa
     6721 ttgctaagtg gtgcaataca caccaacatg acgttgctcc tggtgacgct caacgtttat
     6781 ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacag ttaaagtaag
     6841 tcgaaccact tcatgtagcc gagcaatcaa ttacgcagaa ccacgagcaa ccgtcaaaac
     6901 aggaataaac tgtgacattg attatgccaa aagtgaaatg aaacaaatac gcatgcttta
     6961 tggaaaagac gatcgtgtac aagcacattt attgattcag tcctttcgac caggagaaat
     7021 taccgctgaa aaagccaacc aactagggaa agaatacgct gaaaaaatcg cgccagaata
     7081 ccaaatcgcg atttacacgc acacagataa agagcatatc cacaatcaca ttgtcattaa
     7141 ttcggtcaat ttagaaacag gaaaaaagtt tcaagcacat ggtcaagcat tcctagataa
     7201 gtgttacgat atcaacgacg aaatttgttt aactcatggt ttaagtatca ctgaacgagg
     7261 aaaaaaagaa gaaaaacgaa ccatggctga aatcaaaata aaagaaaaaa atgaacctgt
     7321 ttggaaagat gaaattcggt ttgcgattga tcaaaccatg aaaaacaaga aaaccagaac
     7381 ctatgaccaa ttttgtgatt ccctcaaaac attcggcatt cattgcttca atcgcgggaa
     7441 aaatttcact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca gactaggaaa
     7501 ggactacgaa aaggagacga ttttacatga gctggacaga cgagaacgaa cctataacca
     7561 acagcgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg cagaagaagt
     7621 acgaagagaa caacgaactc ttacgcctga acaaccggaa attagaccgg ctcgaagaaa
     7681 ccatgaacaa cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc tctaaaacgt
     7741 taaaagacat agaaaacgaa ataaagaaac aacgtgcttt tcttgctccc tacacacaag
     7801 aaaggataca aactacgatt gatttacaag ttaaaaaact ccatcaagag ctattagatc
     7861 agcgaaataa agaacaaaaa aaggcgttca ttcaacgatc aatggatttt cttgccccct
     7921 tatgttgctt agtcctgaca ctgttcgcat tttggttagt atggggaggc taaaatggca
     7981 ttttatttac cctatctact catttttgtt agtatttcag gaagtatttg gctcatctat
     8041 aaaattttcc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgatttttc
     8101 ttgcttggct gtatattttc cttgataatc gtttcaagcg gcttattagg cgttttagag
     8161 ggcaataagc gcgtttctag aagtatttta ttaggaaatg tcactcaaaa gtatgaatca
     8221 gcacgtaata aaaagaaaaa agagcaagcc ttagcacaaa aaatagaaaa atttaccgct
     8281 tgttatgagg acatgaatga tatttttgtg aagcaagaaa agcgtttgac ggataaaaat
     8341 atggaaacac tcactcgact ttatcggaat ttgccagaag aaccgcaaaa agaataccaa
     8401 gaaaaatatg aacaagtaaa aaaggacgtg caatatgtaa aagatacaaa gatagaagaa
     8461 gcctgtagtg atctatttgg agacacaaac ccttggtttg ccagtgaaga agaaaaaaaa
     8521 gaaaaacaac aatctgtcac atatgaacgc tacgaaaacc tgtttcaaca agcaactaac
     8581 atacaaagtc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     8641 ttagatcagc aacagcaaaa ttgaatttga tcggcaatca tgttaaacta aacatgcaaa
     8701 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     8761 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     8821 taaagagaca caaacttcta taagaagggg gcaaatgaac aattcaaaca ttagatacac
     8881 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatatctta aaaaattaaa
     8941 cttgtgtggt gtttttaagt atcgcttatt ttagggttgt tagggcttgc cctgaccgcc
     9001 tgtaaggcgc tttaaagcta gataaggatt tttatccaag actgtcttaa aacggcttag
     9061 ataagcattc acaaccgctc aaaggcctct ttaccaacat gtttttagca aaggagcctt
     9121 taggcgactg cgcagtacaa tgtgagagcg tagcgatcac acctttttgg cgaccgtagg
     9181 aagcaaggtt ttggattggc agttttgaaa aatgcgcgaa cctgtttaaa gaaaaaagaa
     9241 gtggttttta ggcgggaaaa ttagcccccc tctttttata tctcttttta aacctctttt
     9301 taaacctctt taccgtaagc tgtgtcctta ctctaccaat ggatacagaa tgttatgaag
     9361 gacattttgt ctgtttatga aggacatttt gtctgtttat gaaggacatt ttgtctgttt
     9421 atgaaggaca ttttgtctgt ttgaacgtca aatatgaatg tcttgacatt taaaaaatgt
     9481 cattcataat tggattgagg tgattat
//
