LOCUS       NZ_LR135240            13173 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7067 plasmid 6.
ACCESSION   NZ_LR135240
VERSION     NZ_LR135240.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884945
            Assembly: GCF_900639335.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13173)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:01:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,115
            CDSs (total)                      :: 3,022
            Genes (coding)                    :: 2,879
            CDSs (with protein)               :: 2,879
            Genes (RNA)                       :: 93
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 143
            CDSs (without protein)            :: 143
            Pseudo Genes (ambiguous residues) :: 0 of 143
            Pseudo Genes (frameshifted)       :: 61 of 143
            Pseudo Genes (incomplete)         :: 82 of 143
            Pseudo Genes (internal stop)      :: 34 of 143
            Pseudo Genes (multiple problems)  :: 27 of 143
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135240.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7067"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            1..933
                     /locus_tag="EQB63_RS16105"
     CDS             1..933
                     /locus_tag="EQB63_RS16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="EQB63_RS16110"
     CDS             950..1420
                     /locus_tag="EQB63_RS16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002318192.1"
                     /translation="MTDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAKTTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1977
                     /locus_tag="EQB63_RS16685"
     CDS             1504..1977
                     /locus_tag="EQB63_RS16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_232043134.1"
                     /translation="MTISKVIKQKRIEKQLTQEDIAEMLLVSKKNISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLREYKIFCVNEKIHTKKEVPSVE"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2017..3195
                     /locus_tag="EQB63_RS16120"
     CDS             2017..3195
                     /locus_tag="EQB63_RS16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="WP_000997695.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDYE
                     KYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTI
                     IHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFMD
                     ATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRGVQEV
                     LLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAVYQANS
                     KEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIESFNKQI
                     KRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE"
                     /name="IS256-like element ISEf1 family transposase"
     gene            3429..4610
                     /locus_tag="EQB63_RS16125"
     CDS             3429..4610
                     /locus_tag="EQB63_RS16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            4700..4969
                     /locus_tag="EQB63_RS16130"
     CDS             4700..4969
                     /locus_tag="EQB63_RS16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 32"
                     /protein_id="WP_002313303.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKVKMLVNIRGKQTMRTCYLMSWTASSRTAKYYYYI"
                     /name="bacteriocin 32"
     gene            5775..5948
                     /locus_tag="EQB63_RS16460"
     CDS             5775..5948
                     /locus_tag="EQB63_RS16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318189.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(6494..7642)
                     /gene="tnpB"
                     /locus_tag="EQB63_RS16135"
     CDS             complement(6494..7642)
                     /gene="tnpB"
                     /locus_tag="EQB63_RS16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7659..8063)
                     /gene="tnpA"
                     /locus_tag="EQB63_RS16140"
     CDS             complement(7659..8063)
                     /gene="tnpA"
                     /locus_tag="EQB63_RS16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            8244..9398
                     /locus_tag="EQB63_RS16145"
     CDS             8244..9398
                     /locus_tag="EQB63_RS16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_002368070.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            9371..9814
                     /locus_tag="EQB63_RS16150"
     CDS             9371..9814
                     /locus_tag="EQB63_RS16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_223917876.1"
                     /translation="MGQYHYKLVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            10117..10497
                     /locus_tag="EQB63_RS16155"
     CDS             10117..10497
                     /locus_tag="EQB63_RS16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_127836249.1"
                     /translation="MSEQNQNSASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            10479..11393
                     /locus_tag="EQB63_RS16160"
     CDS             10479..11393
                     /locus_tag="EQB63_RS16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002303564.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            11353..11631
                     /locus_tag="EQB63_RS16860"
     CDS             11353..11631
                     /locus_tag="EQB63_RS16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_033627308.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLAPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKAFIQRSMDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            11633..12322
                     /locus_tag="EQB63_RS16170"
     CDS             11633..12322
                     /locus_tag="EQB63_RS16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127836250.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESFKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.786"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10480..11390
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaaccata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaacgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgacagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaaaaaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactttatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatatagctg aaatgctttt agtatctaaa aaaaacattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttcg agagtataaa
     1921 atattttgtg taaatgaaaa aatccataca aaaaaggaag tcccttctgt agaataaagt
     1981 taacgacaac caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt
     2041 gtgcaaactc tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa
     2101 gcgataaaca cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat
     2161 cgcactggtt ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc
     2221 gaatatggtg aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa
     2281 actttaccag cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc
     2341 gaaaaaggtg ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac
     2401 tatactccac aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt
     2461 aaatccagag ccttaaatga taagtatgtc gctattttta tggacgctac ttacattcca
     2521 ctaaaacgtc aaactgtatc caaagaagcg atttatattg ccattggtat acgagaagac
     2581 ggcactaaag aagtactgag ttatgcgatt gctccaactg aatcaacata cgtttggaat
     2641 gagctgctac aggatattaa ctccagagga gttcaagaag tcttgctttt tattacggac
     2701 ggcttaaaag gcatgaaaga tactatccat caaatttatc ctaaagcaaa atatcagcat
     2761 tgttgtatcc atgtatctcg taatatcgct cataaagtac gtgtcaaaga ccgaaaagaa
     2821 atctgtgatg actttaaggc tgtttatcaa gctaactcaa aagaagaagc gaataccttc
     2881 ttatccggca tgattgagaa atggaagaaa aactatccta aagtgacgca gtcactcata
     2941 gaaaaccaag acttattaac tttttatgat tttccaccta gcattcgtag aaccatttac
     3001 tcaaccaatc taatcgagtc tttcaataag caaattaaaa gatacagccg tagaaaagag
     3061 cagtttcaaa atgaagaatc actagaacgc tttctagtca gcatttttga tacatacaat
     3121 caaaaatttc taaacagaag ccataaaggt tttcaacagg taaccgatac attagtttca
     3181 atgtttactg agtaactaat tattttgcag gaggacaatt tatttacaca aaattattga
     3241 cgctcccatt tcttcacagc atatttccaa ttgttttatt aatttttcta tactttatat
     3301 aaaaagatag aactatttag taagataaaa aagtagctaa ttgtaatttt cgtctaaaac
     3361 ttaaaactag taaaaataaa tttattatgt tatttttatt tataaattat gaaaaaggac
     3421 ttttcgaaat gaaaaataat agaatctaca aaaatcagtt gaattatggg cttttattgc
     3481 cagtttttct gctatgtatc attggattac tttctttata tgttgcttta tatcacgact
     3541 cttacactaa ttcaaaaatc acaaaagaat taattaaaca agtattgtgg tatagtattg
     3601 ggttcacttc agcaataact atttattttt ttaatcctaa aattttgtgg aaattaactc
     3661 ctatatttta cggattatgc atcataatgt tattacttct cttgaaattc tataatccac
     3721 aattagcagt cttaactgga gcaaaaaatt ggttttatat tgctggtttt tcgtttcaac
     3781 ctactgagtt agtcaaaata ggatacaccc ttatgctagc taaaataacc gtcaattatc
     3841 agcaaaaatc tattactagt aatacgaaag atgattggat ttatttactt aaaactttta
     3901 ttgctactct tccaatagga atattattat tttttcagaa cgatctagga acaatgttag
     3961 tcttcattgt tatttatatt tttattttgt ttacgtctaa cataaatata aaaataattg
     4021 ccccaataat cgccattggt gttgttttat ccttaatatt tttatatttg gttatatcag
     4081 atacaggaag agatttctta ttaaaaatag gatttcacga ctatcagttt aaaagaatag
     4141 actcatggtt gaatccattt ttagatccaa atggatctag ctatcagtta gcaaatagtt
     4201 taattgctat tggctctggc ggacttttag gtactggttt taatgtatca aacattcacg
     4261 ttcctgttag agaatccgat atgatatttt ctgtaattgg agaaaatttt ggctttatag
     4321 gaagttgtct tgtaatattc ctatactttt atttaattta tcaaatgtat aaaacatgtt
     4381 ttaaaagcaa taacttattt ttcacctata tcggtatagg cattgttgca atgatttttt
     4441 ttcatgtttt tgaaaatata ggtgcgagta tcggattact acctctaact ggtattccac
     4501 tcccctatat tagtcaaggt ggttctgcca ttcttagtaa ttttttgggg ttgggattta
     4561 tgttgtgttg tagtagatat actaacgaaa aaaaaggaac acaaaaatga attcaaaata
     4621 acactttatg aatagaatgt ctatttcaat aaaaattatg ataccttaaa gttaatcatt
     4681 aaaaaaggag tgtcataatt tgaaaaaaac aaaattattg gtagctagtt tgtgtttgtt
     4741 tagcagttta ttagctttca ccccttctgt ttcattttct caaaatggtg gagtggttga
     4801 agctgctgct caaagaggat atatctacaa aaaatatcca aaaggtgcta aagtgccaaa
     4861 taaagttaaa atgttagtaa atattagagg taaacaaact atgcgtactt gttaccttat
     4921 gagttggact gctagtagta gaacagccaa atattattac tacatttagt aaaaacaaaa
     4981 cattctttct atacaataaa atagcgtata gagaggatgt ttttaatttg aaaaggagtg
     5041 gttatcttta tgaaaaatag atcatttgct atattagtat tatccgtaat tttattagat
     5101 atattgttaa taacattagc tttaaaaact ttaaatttag taacaagttt tgtcattata
     5161 ccagtaatta taggaatgtc tatttatttc atcaaaaaaa ataaacaatt tttttgaatg
     5221 aaattacggg cgaaaataat cactcaaaat tattaataaa aacgaatcaa ctactttttc
     5281 tgaagaaaca tacaaccgta aacaacaatt gttgtttacg gttgtaaatt atcaaactat
     5341 atggtgactt gttgcttaaa ataggttgaa atttcgttta ttttttttga gaaattttac
     5401 ccacgtacta cccatgtacc tatccacgta ttacccatgt accccccctg acaaatgggc
     5461 ggaggggctt gcggcgcttg gtattaaagg cattctccgc ctcccaggtt tctgaccatt
     5521 ttctctcaac gtggtaaaaa ccccacttat gagagaaaat ggtcagaaac ctaaacgatc
     5581 ggcaaagaaa acataccaag cgccacaagc aaaaaaagtt agagtaactt ccagaaaaga
     5641 aaaatttttt agaacaactt ttgaaaattt gattttgatt tatatccgtt caatttaaac
     5701 atttgataaa ctgaaggcta gtatttaaaa tgtgtataca agtggtagcg ttaaaaaaag
     5761 tggaggtata acctatgtta aaaaaaactg tgttggtact gggttctatt tctctaggaa
     5821 tattaggttt tagccccatg ctattatccg taattggaaa aaacaattcc aacagagaac
     5881 ttaaattaaa ttcaaattat tacaacagtc ctaacgaaga attgtttgta aaagaaacgg
     5941 ataaataata aaaaagactg ctcttccgag cagccagtta aaaaataatg agtgtttatt
     6001 cttgaaaaag caacgcgctt atgaagcgct accaaaaatt tatcatattt tatgaaaaat
     6061 aacaccccag taaaaaatga ctggtaaaaa ttcacaaaga aacggaaaga agcaacggca
     6121 gaaaatcaag ccttaaaaag agcttttgac ctagaccaat taaaaatcac aaagtgagcc
     6181 cctgtatccc ttggtagaga ggctacttgg catagtcgac attatgtcta ctagctcgat
     6241 aaataacgat tatgttccca aaacaatatt ttatgaacta taacaaaaat ttgttatcgc
     6301 atattgtata ttttaattat aggtgtatat cacgtgaact actcaccact tagctaaacc
     6361 tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta
     6421 tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc
     6481 ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat
     6541 tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc
     6601 gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg
     6661 gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt
     6721 tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca
     6781 acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg
     6841 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta
     6901 ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag
     6961 ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg
     7021 acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag tccataatac
     7081 ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct tctttacaca
     7141 acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcagat ttgataatcc
     7201 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
     7261 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
     7321 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
     7381 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     7441 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     7501 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     7561 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     7621 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     7681 ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag aagacaaaaa
     7741 cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa gtcacgtttt
     7801 atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc tgttttaggt
     7861 tgagctttga acataatatg aacgtggtct ttatcatgat tccactccac taaagtaatg
     7921 tgatacgatt ctgaaattct ttcaaaagta gttttggcat aatcagatat ttcatcatca
     7981 atcacttgtc tacgatattt cgttactaaa acaaggtggt aataaagaag aaatactgaa
     8041 tgattatttg tatctaattc catgttatat caactcattt cttgtataga ctgattgtaa
     8101 catggaacaa aataaaaagg caatacgcct tagttgtcgg tcttcgagtt accaacggta
     8161 accctcatac cgaacggcat tcatcaccca cctatagagg atgggcgact tctgcctaat
     8221 tctgttaaat ttaacacaaa ggcgtgatta ttttgaaacc caaaaagaaa atttatctag
     8281 gtctcctctt ttccttgatg attggaattt ttagtatagg aattacaaat acctatgctt
     8341 cagaaaattc taatttcaat atcaactccc aaccaattga agaacaagca attaattttg
     8401 cgaaaactga ttttgaacac tccataactt caattctttt aatggaaaat aacaccgaaa
     8461 ctgatattaa ctatactctt ggttcgccat ttaaaatgct cgatggagaa aatacggcaa
     8521 atagttctat tttctttcca gtattttcag acgctgataa tacgattaaa tatgtttata
     8581 ccgtcaaaaa aaatacaaat ggagaatatt caggaagtat ttcacaattt ttagcaaaag
     8641 aacttcaaaa tctagtagat agtaatataa cagatacaac cttatttttt caagataata
     8701 gcatttatta ttccgataat ggacaaatta aactactttg gaaatcacct ttaagtacag
     8761 aaaaaaatgg tgtttcttcc cttacagaaa aaaatgatct tatatccgta aatataaaaa
     8821 ataaaattaa aaatttttct aaaaaacaaa attttcctgc ggttaaacgt gtaaaaagtg
     8881 tctcctccgg agataacttt attatgatta attggcgtat agcagaaacc caaacaagtt
     8941 ttccgtggtg cgctgcttac gtagaagctg ctatcttatg taacaaaaca gatgtacgtg
     9001 ctacttcagc tagagccatt atgcattata cctatcctaa tttaaacaac agtcaaattg
     9061 aatcaaaaag tcttaatcaa gatcaaataa taaaatatgc aaattactgt ggtgtttatc
     9121 ctaaaaaaat agcacgaata ctaaaccttt cagaggtcca aacacaacta agaaaaggaa
     9181 atgccattta tatgggaact gctggttatg gaaattatag taacagccgt catgcacttg
     9241 cattatttgg gtgggtcaat caaggtggtt atcaaactta ctatgtgtgg aatccttggt
     9301 ataactaccc aactgtaata agtgcaaatg caggaccagt tacaattcct gtacctggag
     9361 gtggttttac ttgggacaat accattacaa attggtaaat aaactcattg ctttagttat
     9421 gggtctagtg ttagttcttt tcttaactag ttgttcaaaa aaacaagaaa tacaaaagac
     9481 tgatctgatt ccttttctag gaacttggca aactaaagat cattcagaac gtctagtaat
     9541 cactaattta aatacttcaa aaaatataga acaatatacc atcttagtaa aagagaacaa
     9601 tcataaaatt caaatcattg ataaaaattt aaatataaat gaacaagttt tcatttacaa
     9661 agcaacaaat aatttactaa cactgcgctc gaataaaaaa caattagatt acacttttag
     9721 tttggattct ccaaaaaagc tcgtatttat tttgacaacg aatgatggga cagaagggac
     9781 agctaaacca attgaatttt ttaaagctga ttgatgaata gttgtttgat attacaaaac
     9841 aataatttca ccgaaggtga aacaagcgaa actttacaaa aagtggctag catttgctag
     9901 ccttttttgt attgtggagc gtgggttttg agggggattc aagggggagc ttgcgcccct
     9961 ttgacaagcg gttatttgca ccaaatacgt aggtatttgg ctagcaaata tagagcttgc
    10021 caaacccttt tagtgaactt tactcattgt acctaaaaag gtacaatgag tattgtgaac
    10081 gatacgtccg taggacgtac agaaagggtg ggacttatga gcgaacaaaa tcaaaattcg
    10141 gctagcgatc cttctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
    10201 gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg aagctttgca aatgagtgtg
    10261 ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
    10321 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
    10381 gctaagtggt gcaatacaca ccaacatgac gttgctcctg gtgacgctca acgtttatcc
    10441 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt aaagtaagtc
    10501 gaaccacttc atgtagccga gcaatcaatt acgcagaacc acgagcaacc gtcaaaacag
    10561 gaataaactg tgacattgat tatgccaaaa gtgaaatgaa acaaatacgc atgctttatg
    10621 gaaaagacga tcgtgtacaa gcacatttat tgattcagtc ctttcgacca ggagaaatta
    10681 ccgctgaaaa agccaaccaa ctagggaaag aatacgctga aaaaatcgcg ccagaatacc
    10741 aaatcgcgat ttacacgcac acagataaag agcatatcca caatcacatt gtcattaatt
    10801 cggtcaattt agaaacagga aaaaagtttc aagcacatgg tcaagcattc ctagataagt
    10861 gttacgatat caacgacgaa atttgtttaa ctcatggttt aagtatcact gaacgaggaa
    10921 aaaaagaaga aaaacgaacc atggctgaaa tcaaaataaa agaaaaaaat gaacctgttt
    10981 ggaaagatga aattcggttt gcgattgatc aaaccatgaa aaacaagaaa accagaacct
    11041 atgaccaatt ttgtgattcc ctcaaaacat tcggcattca ttgcttcaat cgcgggaaaa
    11101 atttcactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaga ctaggaaagg
    11161 actacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc tataaccaac
    11221 agcgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac
    11281 gaagagaaca acgaactctt acgcctgaac aaccggaaat tagaccggct cgaagaaacc
    11341 atgaacaaca cgttgaaaga ggaacagacc accaacaaga attatcgctc taaaacgtta
    11401 aaagacatag aaaacgaaat aaagaaacaa cgtgcttttc ttgctcccta cacacaagaa
    11461 aggatacaaa ctacgattga tttacaagtt aaaaaactcc atcaagagct attagatcag
    11521 cgaaataaag aacaaaaaaa ggcgttcatt caacgatcaa tggattttct tgccccctta
    11581 tgttgcttag tcctgacact gttcgcattt tggttagtat ggggaggcta aaatggcatt
    11641 ttatttaccc tatctactca tttttgttag tatttcagga agtatttggc tcatctataa
    11701 aattttccaa acaagatatt cattaaaagg ctctaaaatt cgatttaagc gatttttctt
    11761 gcttggctgt atattttcct tgataatcgt ttcaagcggc ttattaggcg ttttagaggg
    11821 caataagcgc gtttctagaa gtattttatt aggaaatgtc actcaaaagt atgaatcagc
    11881 acgtaataaa aagaaaaaag agcaagcctt agcacaaaaa atagaaaaat ttaccgcttg
    11941 ttatgaggac atgaatgata tttttgtgaa gcaagaaaag cgtttgacgg ataaaaatat
    12001 ggaaacactc actcgacttt atcggaattt gccagaagaa ccgcaaaaag aataccaaga
    12061 aaaatatgaa caagtaaaaa aggacgtgca atatgtaaaa gatacaaaga tagaagaagc
    12121 ctgtagtgat ctatttggag acacaaaccc ttggtttgcc agtgaagaag aaaaaaaaga
    12181 aaaacaacaa tctgtcacat atgaacgcta cgaaaacctg tttcaacaag caactaacat
    12241 acaaagtcca acgaaaaaag aaacggcatt aaattatcta gaaagtttca aggaatggtt
    12301 agatcagcaa caacaaaatt gaatttgatt taaaatcatg ttaaactaaa catgcaaata
    12361 ggtaatcatg agatacacga aagccctaga gaagtcggat tctctagggc tttttttttt
    12421 ggctgatgtc gccttagttg attttacgtc ttgtttcggt tattcagcca ataactgaat
    12481 agagagttta gaagctcaac aagaagggcg caaataaagt tggtaatcat aagatacacc
    12541 tccttccagt cgcaagactg tcggataggc gacaagttat tatatcttaa aaaattaaat
    12601 ttgtgaggtg tttttaagta ttacttgttt tagggttgtt agggcttgcc ctgaccgcct
    12661 gtagggcgct ttaaattcca gataaaggtt tttatactaa gactgtctta aaacggctta
    12721 gacaagctct tagagcctca accctccttt ttgaacaatg gttttagcaa aggagctgga
    12781 ggcgactgcg cagtataatg tgagagcgta gcgatcacac ctttttggcg accgtaggga
    12841 gcgaggtttt gaattagcag tttagaaaag tgcgcgaacc tgtttaaaga aaaaagaatt
    12901 gttttttagg cgggaaattt tgtctccctc ttttataatc tttttttaaa cctcttttta
    12961 aacctcttta ccgtcggctg tgtccttact cccccaaggg atacaaaatt ttataggcat
    13021 gaaattatct gtttataggc atgaaattat ctgtttatag gcatgaaatt atctgtttat
    13081 aggcatgaaa ttatctgttt taggtattgt ttttagacat aatatgtcct aaaattatgt
    13141 caataaaatc atttagactg gagtaacaaa taa
//
