LOCUS       NZ_LR135234             2056 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7025 plasmid 9.
ACCESSION   NZ_LR135234
VERSION     NZ_LR135234.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884910
            Assembly: GCF_900639355.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:55:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,136
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 80 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135234.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7025"
                     /db_xref="taxon:1352"
                     /plasmid="9"
     gene            21..278
                     /locus_tag="EQB58_RS16380"
     CDS             21..278
                     /locus_tag="EQB58_RS16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(414..1016)
                     /locus_tag="EQB58_RS16385"
     CDS             complement(414..1016)
                     /locus_tag="EQB58_RS16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_010729575.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1083..1238)
                     /locus_tag="EQB58_RS16390"
     CDS             complement(1083..1238)
                     /locus_tag="EQB58_RS16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1855..>2056
                     /locus_tag="EQB58_RS16395"
     CDS             1855..>2056
                     /locus_tag="EQB58_RS16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127824000.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVI"
                     /name="hypothetical protein"
     Typing          181..356
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(487..1016)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ccaccaaaga aggtgagtaa atgcctaaag aaacaactat caacgttgaa caactagaaa
       61 aattgctaga atcatattcc aatgatttta tcgaattgac cggcggaaaa gtcgaagaaa
      121 ccaccgacct ctttgaccta cttctcacaa atattctgga attaaactct gaagaacaaa
      181 aagagcataa gggaaaactg atagcacctt cctacaggaa ggcggctaag tcggctgtgc
      241 caactggttt tcttacaaaa caaccttata ttttttgata tcacaaaaaa atccattttg
      301 gacctatttt cggtcataat atagtaccta cttttggtca tagtttcgtc cgtagttttg
      361 gtcatagtga ctaagacttt cacgcacaaa aaaagagctg aaattaacag ctcttatcct
      421 ttatttattt ttgaatttac acttccacca tgacgattcg attttatata ttgcccgata
      481 atataagctg aattatcaca aagaagcgga aaccaatcat caaatctttc tgacattgca
      541 tagtcaatta aatctttgat ttcagtgata ttcttagaat caacaaacga catcatctct
      601 ctaatcaact catatcgttc tgcactcgtt actgacaaat aactagcaat ttctgcacca
      661 ccatgagcga taatatcact tttagcatat tgaaattttt ctggattatc catatgagca
      721 aaatatcgca ccataccttt tgcattagca catttttgcg ggtttggaga ccgaatcttt
      781 tgagttatct cacgtatttg atcatacgtc tttttaccgc taaacattat caaaacatgc
      841 caatgaggct ttttcaattc accgtcagga ttcacatctt tatcatgaag tggactctca
      901 acccaaggaa catgtaactc atctaaaaac tccctccaat tttcaggagc cgattcagga
      961 tagactacaa acgtccaatt ccttgtgcgt tcatctttag tttttctact cgtcatgcta
     1021 taatcacctt aattgaacaa cagtctaaca gcaaaagcag tgcgccaaca ctaatgcttt
     1081 tgctattttt tttgctcatt ttcctttccg ttagcattaa cccaaattgt aataagacca
     1141 gacttactaa gacccatttg tttcgcaatt ttatcaagat tatctgccac ttccttagta
     1201 agcgttacaa cgactctctt tttttcatta ctgatcataa ttcgtatcct ccaacgactt
     1261 tttcttaaaa accatagtaa caaaaaagta actaaggtgc aacagtttag atactaatta
     1321 gcgaataata ggccttaaac ccttggcatt actgattttg tcaaaaaaaa acgctgaaac
     1381 ccttgggaga gtaaggctaa acagctatcg accaatgacc aatgaccaat aaggggggtg
     1441 tcgtagtccc cccctcctcc gcccaattca taaacaccaa aaccaaaaaa caaacctcag
     1501 ggctccgccc cgaaccccga cctccttcta ccgaaatata tgcaggcgcc acgcttacta
     1561 tttcgttcag ctatcggctt ttgcaatcta acacgaccgt ttcgctacca gtcgccttac
     1621 aggctccatt ctcgcttctc ttacgtgttt taacgattgc gccgataact aaacgaaata
     1681 gacgctaaat cgcttaaaac gcaaaataga ccctttttag agcattgatt ttttctacta
     1741 gtcatttcga taatataaaa ttgaccacaa cacagaaaac ttatattata agcttatgtc
     1801 gtaagtccga cgtactcacg taatatagac ggacgttaaa tggaaggagc ataaatgaga
     1861 gtgataaaat cagaaaaatt cactttggcc gaaattatga cgaccagcga acttgcacaa
     1921 gaacttgatt acaaccaagc ttacgttttg cgcttagcga aagaacatct gactgaagga
     1981 aaagaatatc gctctgccgg tagaagaaac tatcttttca gcccagaagc ccttaaaaaa
     2041 ctagaaaaag tcataa
//
