LOCUS       NZ_LR135233             2947 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7025 plasmid 8.
ACCESSION   NZ_LR135233
VERSION     NZ_LR135233.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884910
            Assembly: GCF_900639355.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:55:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,136
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 80 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135233.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7025"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            17..433
                     /locus_tag="EQB58_RS16360"
     CDS             17..433
                     /locus_tag="EQB58_RS16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322684.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(770..910)
                     /locus_tag="EQB58_RS16365"
     CDS             complement(770..910)
                     /locus_tag="EQB58_RS16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326822.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="WP_002322683.1"
                     /translation="MSDSIEKMTLKKLADELGVSKNTVRNQLVNSGFDLAQYKEKNAVV
                     N"
                     /name="DeoR family transcriptional regulator"
     gene            complement(907..1881)
                     /locus_tag="EQB58_RS16370"
     CDS             complement(907..1881)
                     /locus_tag="EQB58_RS16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_002337993.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2681..>2947
                     /locus_tag="EQB58_RS16375"
     CDS             2681..>2947
                     /locus_tag="EQB58_RS16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127823999.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETL"
                     /name="hypothetical protein"
     Typing          complement(911..1857)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atcggaggaa gataacatga gaaaaacttc tgttcaaaaa cacgttaccc tttatcccag
       61 tcacgtggac gttttagatt ccattatcga aagaaatcca gatatcaaca attactcgga
      121 agccattcgt tttttatgta tgagttatga acctgatacc aatactacga ctaccagcaa
      181 ccaagccaaa ctaaatgcta tgggaaaaga aatttccatc atgtccgaaa tcattgcaga
      241 atttgctgat ttatatctaa aagatacagg tattcttatt ggcacaaatt ctaaagtgta
      301 tcaagaaacg aaagaaaaaa ttgaaaaaca acttaatccc tccaaacgaa atcaaccaaa
      361 aaatcgtcaa aaagaaacca atcaaacatt tacttctaaa aatttcgacc caacaaattt
      421 tctaaaaaga taaaaaataa accaggagat ttaaattcct ggtttatttt ttgggtagga
      481 tcaccatctt aatagcaaca tttacaataa gatagtaacc aattgaatga aaaaaacgaa
      541 tttcagaaga aataacagaa aaaaagataa aaacaaaaaa attataaata catgatttga
      601 attcataatc gtaaaaaaag ctgctcgtac gagcagctaa ataagaaaaa gattggtatg
      661 ttttaaggat aaaataagta taacaaattt ttcacataga gcaaaggggg cgaaaatcgc
      721 cccctttttt tcgttttaaa accccaaaac ccaaagacat aaatttcggt caattgacca
      781 ctgcattttt ttctttatac tgagcaaggt caaatcccga attgaccagt tgattgcgca
      841 ccgtattttt cgatacaccc aattcatcag caagtttttt tagggtcatt ttttctatcg
      901 aatcactcat tcagcaccta cttccgtcac atcaagtcct tgaagccatg tatctaattc
      961 tcgtttcaaa tcatctgccg actccgaaaa tgtttctcgc aaatgattcg tcaactcatc
     1021 attttgactt tcttgcttta acaattcacg atatttatat tttgaatttc tatgaatttg
     1081 cccccagtaa ctatgttccg ttataagagc agtaatcatc attctgtcag ttactttcac
     1141 atcttctggc aaatagtgag gacttgcaaa gctatctaaa ctttcatgaa ccattgcatg
     1201 ccaatcagtt gctttccccc ttcgcaattg caattctagt cgccaccaag tatcgatttc
     1261 tttcggaaca actttccgtt ttctttcttg ttccaatttt ttgttataca tccgaatctg
     1321 tcgttcacta gcacgacttc cccaatatgc tgtttccagt ttcccagacc gaccgtaaat
     1381 tggtttgaaa gaaacaggat caaccacccg atattgggta atgaaatcat ctggcacgtc
     1441 aataatgtca caagcaatat ctgcacgtga gaaatgtggt tctaaaaata aatcatggat
     1501 aaagttcttc atcgagcccg ccagaaattg gctaatttta ttggggttaa agtcaatccg
     1561 cccttttccc tcttgaaact taagaatctc aatataagct atattttcct catgttggtc
     1621 tagtaattgc cagccattat ttccaaccgc ttttaaatat ccgttacctt cgtttagacg
     1681 cataagctgt tcaaaatcta aaatcaacac atcatgggga gtgtgataat aaatattttc
     1741 ccttaatgta ccaacaatgg taattcggtc aattgaccat tgcattttta agggggtgca
     1801 catagtttgt gcatttaacc gcctgttaga tatgcggtta agtttttccc actcatttat
     1861 gatcgtgcca cgtttactca aattttgttc ctcctcaaag aaaaaagaga tttttggaaa
     1921 gaatttccag agttcacgta actctaaaaa tccatatcca aaaatctcta gcttgaagaa
     1981 acctaaaaat attcgtataa tgaatttgag ttacggaact aggtgagtag tttccgtaat
     2041 tagccgaagg gtgcttccaa caccgttcgg cttttatttt gctctttttt ctcgttttct
     2101 ttgattatag cactcctctt aaaaaaataa aaagacctca gggaatttca ttccccgaac
     2161 cccttgagcc tcctcctgcc gaaattattt cacgcttccg ctcttcattt cattcagttc
     2221 tcggcttgca caatctaaca cgccgtttcg ctaccagtcg ccttacaggc tccattctcg
     2281 cttctcttac gtgttttaac gattgtgccg ataactaaac gaaatgaacg ctaaaacgtc
     2341 ttagaacgca aaatacgcgc ttataaaatc atttcatctt gattacatgc ccgtcataat
     2401 cgttcagttt agtttcattt tcgtaaactt tttttatttt tacgaacaga aaaaagtcgc
     2461 acatcaccat tttataaaga gcataaggaa acgtgtaccc actttctaaa aaaagtgggt
     2521 agataggctg tgcctatcac gtttctcaga aaaaaatttg atatcacttt ttcgccatcg
     2581 tggctagctt atgtatagta ttttagcaaa aaacagctat acaaatacta tattcgtatg
     2641 atatacataa attatacaca aatgcgaaag gagtttttct atggacattt tagttcggaa
     2701 gatcgattca aaggttattg caaaaataga caaacgttgc caagaactca caaataaaac
     2761 tggcaaaaaa tggagtcgaa atcgctactt aaaagtccta atcgaaaacg attttgacca
     2821 cgcacttatg cattacaaga aagatcaatt cgatttatta ctcgaaaaat ttatcgctat
     2881 tcaagaaaaa aatactgaaa cgctgaatga atatgttcgt acaaacaacc aactcattga
     2941 aacactt
//
