LOCUS       NZ_LR135232             7165 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7025 plasmid 7.
ACCESSION   NZ_LR135232
VERSION     NZ_LR135232.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884910
            Assembly: GCF_900639355.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:55:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,136
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 80 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135232.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7025"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            <1..625
                     /locus_tag="EQB58_RS16310"
     CDS             <1..625
                     /locus_tag="EQB58_RS16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127823998.1"
                     /translation="KIFQTRYSLKGSKIRFKRFFFLGCIFSLIIVSSGLFGVLEGNKRV
                     SRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCYKEMNDIFVNQEKRLTDKNMEKL
                     TRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEACSDLFGDTNPWFASEEEKKEKQ
                     QSVTYERYENLFQQATNMQNPTKKETALNYLKSVKEWLDQQQQN"
                     /name="hypothetical protein"
     gene            complement(746..832)
                     /locus_tag="EQB58_RS16315"
     CDS             complement(746..832)
                     /locus_tag="EQB58_RS16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_109746925.1"
                     /translation="MFEQFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1477..2214
                     /locus_tag="EQB58_RS16320"
     CDS             1477..2214
                     /locus_tag="EQB58_RS16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            2243..2764
                     /locus_tag="EQB58_RS16325"
     CDS             2243..2764
                     /locus_tag="EQB58_RS16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_031592680.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEQENDKNVYISRKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEVLTSVNKENNNAI
                     TTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            2848..3300
                     /locus_tag="EQB58_RS16330"
     CDS             2848..3300
                     /locus_tag="EQB58_RS16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_025089292.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKVMLKNHTKTLILLFHFVIFLSLVFTLIQSKFFYFSHS
                     IATWLLIFFLSLLLVINTLIIIFNKHKNLFLHSVFPIILLSFLYFV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3711..4865
                     /locus_tag="EQB58_RS16335"
     CDS             3711..4865
                     /locus_tag="EQB58_RS16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            4838..5281
                     /locus_tag="EQB58_RS16340"
     CDS             4838..5281
                     /locus_tag="EQB58_RS16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            5585..5965
                     /locus_tag="EQB58_RS16345"
     CDS             5585..5965
                     /locus_tag="EQB58_RS16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5947..6861
                     /locus_tag="EQB58_RS16350"
     CDS             5947..6861
                     /locus_tag="EQB58_RS16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6821..7099
                     /locus_tag="EQB58_RS16930"
     CDS             6821..7099
                     /locus_tag="EQB58_RS16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     Typing          1478..2214
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5948..6858
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaattttt caaacaagat attcattaaa aggctctaaa attcgattta agcgattttt
       61 ctttcttggc tgtatatttt ccttgataat cgtttcaagc ggcttattcg gcgttttaga
      121 gggaaataag cgcgtttcta gaagtatttt attaggaaat gtcactcaaa agtatgaatc
      181 agcgcgtgat aaaaagaaaa aagagcaagc attagcacaa aaaatggaag agtttaccac
      241 ttgttataag gaaatgaacg atatttttgt gaatcaagaa aaacgtttga cggataaaaa
      301 catggaaaaa ctcactcgtc tttatcaaaa cttgccagaa aaacagcaaa aagaggttca
      361 agacaactat gagcgaacca aaaaagacgt gcaatatgta aaagatacaa agatagaaga
      421 agcctgtagt gatctatttg gagacacaaa cccttggttt gccagtgaag aagaaaaaaa
      481 agaaaaacaa caatctgtca catatgaacg ctacgaaaac ctgtttcaac aagcaactaa
      541 catgcaaaat ccgacaaaaa aagaaaccgc cttaaactat ctaaaaagtg tcaaggaatg
      601 gttagatcag caacagcaaa attgaatttg attgaaactc atgttaaact gaatgtgcaa
      661 atgatcaaac attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttt
      721 caggttgatg tcgcctttac tgattttact tcttgtttcg gttattcaac caataactga
      781 ataaagagac acaaactcct acaagaaggg ggcaaatgaa ctgttcaaac attagataca
      841 cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attataccct acaaaattaa
      901 atctgtgtgg cgattttaag tataccttat tttcgggttg ttagggcttg ccctgaccgc
      961 ctgtaaggcg cttctcgact agattaaggg agttaatcaa gaattgtctt agaacggctt
     1021 agacaagctc tcagattctc ccacctcctc tttgaacaac ggttttagca aaggagccgg
     1081 aggcgactgc gcaggtacaa tgtgagagcg tagcgatcac acctttttag cgcccgtagg
     1141 gagcaagttt tctagtttag aaaaattcgc gaaccttttt ggaggtaaaa gtaggagttt
     1201 tttaggcggg aaattaaatg tgctcttaag atatactgtt agatatactg ttagatatac
     1261 tgttaataat aactgttagg gattagcaac cttatgggag agtaagtatt gacataggta
     1321 gtgggggata aattgtcaca ctatggggga taaattgtca cactatgggg gataaattgt
     1381 cacgctatgg gggataaatc gtcacactat gggggataaa ttgtatttta caccctctta
     1441 tgtctatgat aacataattt attgaggtga tttttattga gtaacgatat agttcgatat
     1501 aataatggat taaattctgt ccctttaaga aaatttaccc ctatagatat ggatttattt
     1561 tggggaatat gttctaaaat gaaaagaaaa ggtacaaaag aagaaatttt tacatttaaa
     1621 caaataaaag aattaattga ttacaatata aatgcacgtg gcgaccgttt tgctaatgat
     1681 ttaataaaaa tgtcaaaaaa aatcattact ttgtctttta tttatgaaga cgaaaaaaca
     1741 tatgaacaat taaatctgtt tcaaaaattt aaaatcgata aagtgaatga aacattatta
     1801 ataaaggttt ctgaagaatt tgaatatata cttaattcaa tcggaactaa ttttactcgt
     1861 ttcgaattag aaaatatgac aaacttaaag agtagctacg ttaaagagtt atatcgccaa
     1921 cttatggctc ataaagacag aagatctaaa aaaggtcact ggtttgtaaa aatagaagat
     1981 tttagagctc tattagcaat accagaaagt tatcgcatga gagatattga taaacaaatt
     2041 tttgcccaag cgaaaaaaga atttttaaac gttaattcaa ctcaccctgc tatttttaaa
     2101 acttttgaaa tcaaaaaaat taaagcctac aaaggaaata agataggatc tctgcaaatt
     2161 tattttgaag aaaatatacc ttcaattacc ttagataatt ggttagagaa ataataacct
     2221 aacaatttag gaggcaatta tcatgcaacc caaagaaacc tttacaataa aggaattatc
     2281 tgaattattt gaaataagta ggcaagcaat ttctaaacat gttgataaat tagacccgtc
     2341 cttattagct aaaaatgagc gtggttataa ggttgtatta cgttctggag tattgcaact
     2401 tgctagcaac ttaggtaacc aaaggttact agataggttg catgagtatg aacaagaaaa
     2461 cgataaaaac gtttatatat caagaaaaaa cgtggttgta aacgagttgc tagataagtt
     2521 gcgtgaagaa aatgaatttt tacgagaaca actttctctc aaagagcaaa ataattctaa
     2581 tatgcaaaaa ttactcgacc agcaacaaca actcaccttg caggcaaatc aacagatcga
     2641 aaaactgcaa gaacagttac aattagctta tacggaagaa cctgaagaag aggtattaac
     2701 ttctgtcaat aaagaaaaca acaatgcaat aacaacagaa aaaaagtggt ggaaattttg
     2761 gtaacaatta ccaactttcc ataaagaaga aatcaatttc tatagacttt atctttctaa
     2821 aaataaaaaa tatgtttagg tgatcaaatg actataagta aagtaatcaa acaaaaaaga
     2881 atagaaaaac aactaactca agaagattta gctgaaatgc ttttagtatc taaaaaaacc
     2941 atttctaatt gggaaaatgg tagaacaata ccagatacag aaaatttaat tcaatttgct
     3001 aagttatttg atttatcact agacaatatt ttgttaggag aagaaaaagt tatgttaaaa
     3061 aatcatacaa aaacactaat actattattt cattttgtaa tttttttaag tttagttttt
     3121 accttaattc agtccaaatt cttttatttt agccattcaa ttgccacttg gttattaata
     3181 ttttttctaa gtttgctatt agtaatcaat actctgatta ttatttttaa taaacataaa
     3241 aatttatttc ttcatagtgt ttttccaatt attttgctga gttttttata ctttgtataa
     3301 agtataaaaa aagactgctc tttcgagcag ccagttaaaa aaataatgag agtttattct
     3361 tgaaaaagca acgcgcttat aaagcgctac caaaatttta tcatatttta taaaaaataa
     3421 caatccagta aaaaatgact ggtaaaaatt cacaaagaaa cggaaagaag caactgcaga
     3481 aaatcaagcc ttaaaaaaag cttttgacct agaccaatga aaaatcacaa agtgagcagc
     3541 tatagccctt gatagagggg ctacttggca tagtcgacat tatgttaact aacgctataa
     3601 ataacgatta tgttcccaaa acaatatttt atgaactata acaaaaattt gttatcgcat
     3661 attgtatatt ttaattatag gtgtatacca cttaaattta acacaaaggc gtgattattt
     3721 tgaaacccaa aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta
     3781 gtataggaat tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac
     3841 caattgaaga acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa
     3901 ttcttttaat ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta
     3961 aaatgctcga tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg
     4021 ctgataatac gattaaatat atttataccg tcaaaaaaaa tacaaatgga gaatattcag
     4081 gaagtatttc acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag
     4141 atacaacctt attttttcaa gataatagca tttattattc cgataatgga caaattaaac
     4201 tactttggaa atcaccttta agtacagaaa aaaatggtgt ttcttccttt acagaaaaaa
     4261 atgatcttat atccgtaaat ataaaaaata aaattaaaaa tttctctaaa aaacaaaatt
     4321 ttcctgcggt taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt
     4381 ggcgtatagc agaaacccaa acaagttttc cgtggtgtgc tgcttacgta gaagctgcta
     4441 tcttatgtaa caaaacagat gtacgtgcta cttcagctag agccattatg cattatacct
     4501 atcctaattt aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa
     4561 aatatgcaaa ttactgtggt gtttatccta aaaaagtagc acgaatacta aacctttcag
     4621 aggtccaaac acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa
     4681 attatagtaa cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc
     4741 aaacttacta tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag
     4801 gaccagttac aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt
     4861 ggtaaataaa cccattgctt tagttatggg tctagtgtta gttcttttct taactagttg
     4921 ttcaaaaaaa caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac
     4981 taaagatcat tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca
     5041 atataccatc ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa
     5101 tataaatgaa caagttttca tttacaaagc aacaaataat ttactaacac tgcgctctaa
     5161 taaaaaacaa ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt
     5221 gacaacgaat gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg
     5281 atgaatagtt gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact
     5341 ttacaaaaag tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg
     5401 gggattcaag ggggagcttg cgcccctttg aaaagcggtt atttgctagc caaatacgta
     5461 ggtatttggc tagcaaatat agagcttgcc aaaccctttt agtgagcttt actcattgta
     5521 cctttttagg tacaatggta ttgcgaacga tacgtccgta ggacgtactg aaagggtggg
     5581 acttatgagc gaacaaaatc aaaatttggc tagcgatcat tctaaaaaat atacctatcg
     5641 atccgagcca aaacaaatca gttttcgagt gagcgaatct gaatttgcaa agctgaagca
     5701 gtcagctgaa gctttgcaaa tgagtgtgcc ggctttcgta aaagccaagg cacaaaacgc
     5761 gcgtctggtg acaccgaaag tggcgccaga cattgcccag gcaatcgcgc gcgatttagc
     5821 caaagctgga gggaatatca atcaaattgc taagtggtgt aatacacacc aacacgacgt
     5881 tgctcctgga gacgcccaac gcttatccga aaacctaaaa atcatgcaaa aggagctaca
     5941 aaagatatgg caacagttaa agtaagccga accacttctt gtagtcgtgc aattaattac
     6001 gctgaaccac gagcaaccat taaaacagga ataaactgtg acgtcgatta tgccaaaagt
     6061 gaaatgaaac aaattcgcat gctgtatgga aaagacgacc gtgtacaggc acacttattg
     6121 attcaatctt ttcgaccagg agaaattacc gctgaaaaag ctaatcaact aggggcagag
     6181 tatgcaaaaa aaatagcccc cgatcaccaa atagctattt acacacatac agacaaagac
     6241 catattcata atcacattgt cattaactcc gttaatctgg agactggaaa aaaatttcaa
     6301 gctcatggtc aagcatttct aaacaaatgt tatgacctaa atgacgagat ttgtcttgca
     6361 cataacttac caatcactga gcgaggaaag aaaccagaaa aacgaaccat gtctgaaata
     6421 aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa ttcggattgc gatcgatcaa
     6481 acaatgaaaa acaaaaaaac cagaacctat gaccaatttt gtaattccct caaaacattg
     6541 gggattcatt gcttcaatcg cgggaaaaat ttcacttacg aattgatcca caaaaagaaa
     6601 aaagtacgat ccaacagact aggaaaggac tacgaaaagg agacgatttt acatgagctg
     6661 gacagacgag aacgaaccta taaccaacag cgaaccaacg agctacgaag agctcgagct
     6721 agcattcgcg aaactgcaga agaagtacga agagaacaac gaactcttac gcctgaacaa
     6781 ccggaaatta gaccggctcg aagaaaccat gaacaacacg ttgaaagagg aacagaccac
     6841 caacaagaat tatcgctcta aaacgttaaa agacatagaa aacgaaataa agaaacaacg
     6901 tgcttttctc gttccttata cgcaagaaag gatacaaact acgattgatt tacaagttaa
     6961 aaaactccat caagagctat tagaccagcg aaataaggaa cagaaaaagg tgcttattca
     7021 acgatcattg gattttcttg cccccttatg ttgcttagtc ctgacactgt tcgcattttg
     7081 gttagtatgg ggaggctaaa atggcatttt atttacccta tctactcatt tttgttagta
     7141 tttcaggaag tatttggctc atcta
//
