LOCUS       NZ_LR135231             9550 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7025 plasmid 6.
ACCESSION   NZ_LR135231
VERSION     NZ_LR135231.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884910
            Assembly: GCF_900639355.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:55:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,136
            CDSs (total)                      :: 3,044
            Genes (coding)                    :: 2,921
            CDSs (with protein)               :: 2,921
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 44 of 123
            Pseudo Genes (incomplete)         :: 80 of 123
            Pseudo Genes (internal stop)      :: 27 of 123
            Pseudo Genes (multiple problems)  :: 25 of 123
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135231.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7025"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(85..501)
                     /locus_tag="EQB58_RS16260"
     CDS             complement(85..501)
                     /locus_tag="EQB58_RS16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806983.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(503..784)
                     /locus_tag="EQB58_RS16265"
     CDS             complement(503..784)
                     /locus_tag="EQB58_RS16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(1231..2199)
                     /locus_tag="EQB58_RS16270"
     CDS             complement(1231..2199)
                     /locus_tag="EQB58_RS16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(3528..3962)
                     /gene="bacA"
                     /locus_tag="EQB58_RS16275"
     CDS             complement(3528..3962)
                     /gene="bacA"
                     /locus_tag="EQB58_RS16275"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(4367..4540)
                     /locus_tag="EQB58_RS16595"
     CDS             complement(4367..4540)
                     /locus_tag="EQB58_RS16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318189.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5221..5760)
                     /locus_tag="EQB58_RS16285"
     CDS             complement(5221..5760)
                     /locus_tag="EQB58_RS16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(5776..6699)
                     /locus_tag="EQB58_RS16290"
     CDS             complement(5776..6699)
                     /locus_tag="EQB58_RS16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     gene            complement(7828..7983)
                     /locus_tag="EQB58_RS16600"
     CDS             complement(7828..7983)
                     /locus_tag="EQB58_RS16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846310.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRIF
                     EQRKKM"
                     /name="hypothetical protein"
     gene            complement(8002..8571)
                     /locus_tag="EQB58_RS16300"
     CDS             complement(8002..8571)
                     /locus_tag="EQB58_RS16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_002322960.1"
                     /translation="MTIKWIISIAYLVLTFAIPSLGVPSNIYFLFEHSDIFFLVLIIIL
                     FHSTFIREFKEVNLKKLFKYSFFSFSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAA
                     TYGAFLEEGIFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQ
                     TKNIWYSIVAHGFYNTIGILIYLISI"
                     /name="type II CAAX endopeptidase family protein"
     gene            complement(8571..9026)
                     /locus_tag="EQB58_RS16305"
     CDS             complement(8571..9026)
                     /locus_tag="EQB58_RS16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LMxysn_1693 family intestinal colonization
                     protein"
                     /protein_id="WP_002322959.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDDE
                     YYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIARNT
                     SASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
                     /name="LMxysn_1693 family intestinal colonization protein"
     Typing          complement(1236..2199)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.679"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5777..6699)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agttggtacc aacttgcaaa tcttgctgga agactgatcc ctaaaaagaa atcgaggaat
       61 ttttttaggg atcggtttct ttttttatct ttttagaaag tttgttgggt caaaatttcc
      121 agaagtaaag gtttgattgg tttctttttg acgatttttg ggttgatttc gtcgggaagg
      181 attgagttga ttttcaattt tttctttcac ttcttgatac actttagaat ttgtaccgat
      241 aagaacacca gtatctttta gatataaatc agcaaattct gccatgattt ctgtcatgat
      301 agaaatttct tttcccatgg cattgagttt tgcttggtta ctggttgtga ttgtattggt
      361 atcaggttca taactcatac ataaaaaacg aatggcttct gaataattgt tgacgtctgg
      421 atttttttcg atgatggaat ctaaaacgtc aacgtgactg ggataaaggg ttacgtgttt
      481 ttgaacagaa gtttttctca tgttatattc ctccgataag tgtttcaatg agttggttgt
      541 ttgtgtgaac atagtcattc agtatgttcg tattttctgt ttgaatggca ataaattttt
      601 cgagtaataa atcgaattga tcttttttgt aattcataag ttgacggtca aaatcacttt
      661 cgattagaac ttttagatat ttatttcgac tccatttttt gcccgttttg gttgtgagtt
      721 cctgacaacg tttatctatt cttgcaataa ttttgggatt gatatttcga actaaaatgt
      781 ccatggaaat tttcctttcg tatcagcgta taatttatgt atatcataca aatatagtat
      841 ttgtatagct atttttcgcc aaaacactat acatagccta gccacaatgg caaaaaagtg
      901 atagcaaatt tttttctgag aaacgtgata ggcacagcct atctagcacc ttttgtaaga
      961 aagtgatacc acgtttcctt atgctctttc aaaattcaga aaaatcagct gtaaaacgag
     1021 tgcagtccga gtaagattca acgaaaaaac ggggattggg gcgccaccaa aatatcgcaa
     1081 catgggtcca tgttgctctg ctcgcaaaag caaaaagaag aataaaacca gcaatttaaa
     1141 ttaataaaaa ttgctggttt tattgccaga attgccacca ttttttctcc gtctttgatt
     1201 gttttttctg tcataaaatc taaaaacccc tcaatctttg aataagtttt gaagattgtc
     1261 taataagtct ttttttagtt ctgaaggaat atcggaattt atctctgatt tttgtgcatt
     1321 attttcttta ttttgggtat tttgttctct ttctaacttt tctaactgtt gtaaaagttc
     1381 caattttcgc ttttgttctt tgattttttc ttgtaatgaa gtaggtgctt tttcagattg
     1441 aacttgttgg ttagctagtt ctaatgcttt gttttgttct tcttttgttg gggcatgttt
     1501 gttaaagtcg tctgattttt ttatctcagg tttaaatgta aattgatagc caatgacagg
     1561 ctttccccgt ccttttccgt atttcttttt gattgttaaa cctttaaaga ttgcagttag
     1621 ttcttgtcgt attggattga acacacgttg atcaatcgta ccttggttgt aacttttagg
     1681 aatatcaagt aattcatgaa agtcttcttt tgaaaaataa gcgaaacctt tggttctgta
     1741 ttgttttagt aatcgaaaca ttgtttttga ataactgctt tctaattcat tgaattgttg
     1801 gagagaatag cgcacccatt cttcaagatt attaagtaag ggtaaggctt tttcatgaac
     1861 ttgtatttca gcaaaaggaa tatcagattt tccgtcgatt ttaaattgat taaataaaac
     1921 aaaacgttca cgttgcaaac cgtcagcact tcttctacca aaacgtaaat ccattaattt
     1981 gtcatacgtt gtttctaaat catctatgaa tcgaacattt gcagttgctt tataattgct
     2041 taaatctttt aattgctcga atgtaaattg tactttttta tcacctacat cacgcattct
     2101 tgatacgata gaaaagaaca aattcatttc gactggtgta aattttcgca acggaattgt
     2161 atttaattca tgatgatact ttacgatttc attagacata tttaaaatag ctcctttctt
     2221 actcgcacaa gagatacacg ttatgaaata caaaatgcct atttatctac atcttatatt
     2281 aacatcgtcg atgtactaat tcaatgtaca aacagacaaa acgatgtaga taaacagaca
     2341 aaacgatgta gataaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     2401 agcgtcattc ccttgggaga gtaaggatat agccgacggt aaagaggttt aaaaagaggt
     2461 ttaaaaagag attataaaag agggggacaa aatttcccgc ctaaaaaacc acttcttttt
     2521 tctccaaaca gattcgcgca tttttctcaa ctgggaatct tactccctac agtcgcttaa
     2581 aaaggtgtga tcgctctgct ctcacattat actgcgcagt cgcttccggc tcctttgcta
     2641 aaaatattca taaggaactc tttgagaagc tatgagcctt cgtttgagac gttttaagac
     2701 agtcttagta taaatccctt atcttgttct taaagcgcct tacaggcggt cagggcaagc
     2761 cctaacaacc ctaaagttgt ctaaatgccc tctaaaattc gatttaagca ctcttaaacc
     2821 tattcttata tatttatata cttaggcgtt tcaaacgtct taaaacgcaa attagagctt
     2881 gataaagata tttgctcgtg tgcctagact tttaaaaaag tcggcacacg ccatgacaaa
     2941 gctgaaataa actgcttcag ctttgtcatg gcggattcgc tcacttgaaa aagtgtttat
     3001 ttcgctcgat tattagcata tattttttag cttttttgct agccaaattt tgcattaatt
     3061 tgctcataag tcccaccctt tcagtacgtc ctacggacgt atcgttcgta atgtgaaagt
     3121 acctttaaag gtactttcta caaaactcac taaaagggtt tggcaagctc tatatttgct
     3181 agccaaatac ctccgtattt ggtgcaaata accgcttgtc aaaggggcgc aagctccccc
     3241 ttgaatcccc ctcaaaaccc acgctccaca atacaaaaag tggctagaaa atgctagcca
     3301 ctttttgtaa aatttagtat gatttatttt tagctattta gagtttttat ttccgtaaat
     3361 acttctaaaa attgctaaga taaatgctat aggaattaga taaagaatac ttttttgtaa
     3421 gcttatattg aaaattagcc taaatatgaa gaacatagtg attaaaacta ttattgaaat
     3481 aattacagct aaaatctttt tattcatgag atagtttcac tttctgatta ttttattaca
     3541 taacgagcgt aatcattgta ttgatatgat gttgtattgc taaagtgagc attatatgtt
     3601 aattgtacac cttttccttg atttgcagct cttacaaaga cattcattgc atcaacagca
     3661 ctactaaaag catttgtaat aacccatgtt ccaactccac caataggaat aaaagatgca
     3721 atttgagcag gagtctttag cttattataa ttttttttca aatctctaca cattttatta
     3781 gtaacataat agttcatttt atagcaagta aatccacgat gattatgatt atatcgtgaa
     3841 cttgcagctt ccacttttat acctctagca ctaaaaggaa acagatttaa cggagtactt
     3901 gctccaacaa tcataccgaa caaaaataga caagtaataa ttttaataat tttatttttc
     3961 attgttactt cctccttaga tataaaatcc taggaaagcg ctttctgtat atatttagaa
     4021 taatcagaaa attaaatatt gtcaagaggg tttattttat aaaacgtgtt attttaaagg
     4081 ttagtagaca taatgtatag tatgccaagt agcccttgta tcaaccgata caggggctca
     4141 ctttgtgatt tttaattggt ctaggtaaaa agctcttttt aaggcttgat tttctgcagt
     4201 tgcttctttc cgtttctttg tgaattttta ccagtcattt tttactggat tgttattttt
     4261 cataaaatat gataaatttt tggtagcgct ttataagcgc gttgcttttt caagaataaa
     4321 cactcattat tttttaactg gctgctcgga agagcagtct tttttattat ttatccgttt
     4381 cttttacaaa caattcttcg ttaggactgt tgtaataatt tgaattcaat ttaagttctc
     4441 tgttggaatt gttttttcca attacggata atagcatggg gctaaaacct aatattccta
     4501 gagaaataga acccagtacc aacacagttt tttttaacat aggttatacc tccacttttt
     4561 ttaacgctac cacttgtata cacattttaa atactagcct tcagtttatc aaatgtttaa
     4621 attgaacgga tataaatcaa aattaaattt tcaaaagttg ttctaaaaaa tttttctttt
     4681 ttggaagtta ctctaacttt ttttgcttgt ggtgcttggt atgttttctc cgcctcccag
     4741 gttcttgacc attttctctt ataagtggga tttttaccac gttgagagaa aatggtcaag
     4801 aacctgggag gcggagaatg cccttaatac caagcaccgc aagcctttcc catactttat
     4861 tcgatgatcc cacgatgatc tcataatgat tctacaatga ccctacgatg atcaaaattc
     4921 tcaaaaaaaa taaacgaaat ttcaaacgat ttttttgaaa ttttgttaaa atcacatttg
     4981 tttttttatg actttttcaa caaaatcgat tagaatttat tttgtttttc caccaaaaat
     5041 tgagtgagat ttcaacctat tttaagcaat aagtcaccat atagtttgat agtttacaac
     5101 tataaacatt gattgtttac agttgtaaac tattttttct ttctcataaa aatttgagtg
     5161 atctccccat caggtagtac aactttttcc tctactatcc gaactaactt ctagaaaatt
     5221 ttagagattg gttctattag atttccaaat ttgccaccac tttttttcaa taagtggttg
     5281 tgtattgtct gctttttttt cagaaaaggc agttgattcg gttgttggag tttcttctgt
     5341 ataagttaaa tggagctgtt cttgtaggtt ctcaatttgt tggttcgctt gtagagtgag
     5401 ttgttgttgc tggtcgagta atttttgcat ttgttttatt tgattatctt tttcttgtag
     5461 ctgtatatct tttcgattta actgtttttc taggaaagaa aggagtttta attcggaaac
     5521 ttggttggtg tttttatggt ttttggtttt tgatttttcg ccaactagtt ttcgatttaa
     5581 gtttgcgtta tcgactaaaa acattgattt tatagctttt tgaccggttt cgttaatcac
     5641 aaacttgttt ccgttttttg caaaccaaag ttttcgattt ttgtcagaaa cttttttgtt
     5701 aattgctgtt ttactaactc ctaattcttc cgcaagttct ttgattgttt tatctgccat
     5761 aaatactcct taagcttaat cgaataagtt ttgtaaattt cctagtaaat cttgtttgag
     5821 tttgtttgaa agttctcgtt tttctgcctg tttttcttca gtttgttgtt gttgtttaaa
     5881 atcagcttca tgagtgccta agggaagatt tagtattcga tctttagcgc gccatttttc
     5941 ttctcgtgtt aattctccat tgtgatcaat attaaataat tttatacgta aatcttcttg
     6001 tttccctttg gaaaaatcgt cagcatggtt aatttcagct ttccaagtaa aacgataacc
     6061 gataacaggt ttacctcgac ctttaccata ttttttacga atagttaatc ctttaaataa
     6121 gggagtgagt tcttctttaa ttggccttaa gataaatttg tcaatatttg acggactatt
     6181 ccaataactt ttaggaatat ctagtaaatc aaaaaatttt tctttggaaa attcagtcca
     6241 gccttgagtt cgatattgtt ttaataatcg aaacatagtt tttgaatatg tgctatccaa
     6301 attagtaaat tgctctagat taaatcttgt ccagttcttt aattgattaa aaagtccttt
     6361 aaattctgga ttaactgaaa ttataacaag ttgtttatct cgtataatgc tatattttgt
     6421 gaaagcaaca aatttagtta aagtatttcc atcatcattc caagcattaa tacttaataa
     6481 tttgtcatac gtgctactaa gatcttttat aaattgttcc ccacgttctg tataaccgct
     6541 tagttttttc aaatcgtgaa atgaaaaaat aatctcatct acaccttttt cacgtacacg
     6601 tgaagcaatt gaaaaaaata agttcatttc acgactatta aattttctca gaggaatatg
     6661 atttaatcta ttttcatatt ttacaatttc atttgccata agcttctcct acttttataa
     6721 agtatgaaat aaaagtaaca tactttataa aaatactcaa taatgtcgga aaacctactt
     6781 gaaaagcgta gaaaacctac ttgaaaagcg tagaaaacct acttgaaaag cgtagaaaac
     6841 ctacttgaaa aaactctgaa ttgcttggga gagtaaggac acagccgaca taaaagagat
     6901 ataaaaagag atataaaaag agatataaaa agaggggagg ctaattttcc cgcctaaaaa
     6961 atcactcctt ttttctctaa acaggttcgc gaatttatca aaactgccaa ttccaaacct
     7021 tgctccctac ggttcgctaa aaaggtgtga tcgctctgct ctcacattat actgcgcagt
     7081 cgcctccggc tcctttgcta aaagccgttc ataaggaacc ctttgagaag ctctgagcgt
     7141 tcgtttaaga cattttaaga cagtcctagt ataaatccct tatcttgttc ttaaaacgcc
     7201 ttacaggcgg tcagggcaag ccctaacaac cctaaaaatt gtctgaatgc cctctaaaat
     7261 tcgatttaag cactcttaaa cctattctta tatatttata tacttagacg tttcaaacgt
     7321 cttaaaacgc aaattagagc ttgataaaga tatttgctcg tgtgccgact tttttaaaag
     7381 tctaggcaca cgccatgaca aagctgaaat aaactgcttc agctttgtca tggcggattc
     7441 gctcacttga aaaagtgttc atttcgctcg attattagca tatatttttt aacttttttt
     7501 gctagccaaa ttttgcatta gttcgctcat aaatcccacc ctttcagtac gtcctacgga
     7561 cgtatcgttc gtaatgtgaa agtaccttta aaggtacttt ctacaaaact cactaaaggg
     7621 tttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa taaccgcttg
     7681 tcaaaggggc gcaagctccc ccttgaatcc cccctcaaaa cccacgctcc acaatacaaa
     7741 aaaggctagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     7801 ttgttgcttt gcaacgatca ggtagagcta catttttttt cgttgttcaa atattcttac
     7861 gccatttact attaaaatcg taggtattcc aaagtaaaga aaaaattgat tagcaggtaa
     7921 tacaaaatat aaaataatac cagcaataat taccaaactt atagtaagtt ttgttaattt
     7981 catgaagtat gccaccttat cttatataga tatcaaatag attaatatgc cgattgtatt
     8041 ataaaatcca tgagcaacta tggaatacca tatatttttt gtttgtatat aaacaaaact
     8101 taaaataata ccaataaaga aaatacttag gcctccccca tgaacaatag aaaacagaaa
     8161 agaagaaatt agtattgaaa tatattgttg ttttttgttt gcttgaggat caatcataca
     8221 aaatcgaaaa attccttctt ctaaaaaagc tccataagta gcagcattta aaaagaataa
     8281 aaggagagaa ccatttactt cattaggatt tataccttct gaaaaagatg tatttagaat
     8341 attgattagg aataaaacag agaatgaaaa aaaactatat ttaaagagtt tttttagatt
     8401 tacttctttg aattcacgaa tgaaggtaga atgaaatagt ataattatta gcactaaaaa
     8461 aaatatatca ctatgttcaa ataaaaaata tatatttgac ggtactccta atgatgggat
     8521 agcaaaggtc aaaaccaagt aagcaatact aattatccat tttattgtca ttaggcagga
     8581 atataatcaa tatattgtgt atttgctgta gaagtttgag tacgaatata tttattatct
     8641 accttacgat aaactctgta ggttacagtt attcttgcat tttgacgata ttttttaaat
     8701 tttccacttg cagaagtgtt tctggctatt tcgattttca tcccaaatac atttgcactg
     8761 acagataact ttgtacctcg actatgagtt tctataccta aataagtacg gcttccttta
     8821 gcaattgacc agctttgagg tttagaccaa gtccttgttt gaataactgg ttcgtctaac
     8881 acatagtagt actcatcatc agaataagtg gctatatcag aaatgttgtt ctcattagcg
     8941 aataccgctc cgctaggagt aatagcagaa ccaaaagtta aaatagaagt agataataaa
     9001 aaggttttaa ataactgttt tttcatcaaa atcacctcga atataagata cttaagaaaa
     9061 cgctttcttt atataattag aataatcagg aaatttaatg ttgtcaagag ggtttatttt
     9121 ataaaagctg ttatattaaa ggttagtaga cataatgtat atactaccaa gttgcccctc
     9181 taccaatgga tagagacgct aactttgtga tttctaattg gtctagctta aacacttcag
     9241 ccaatgcttg attttatgca cttttctttt ttcatttctt tgtgcatttt gaccggtcat
     9301 tttttagaga tttgttattt ttgtgagcat ttgatatgct ctttttatac ctcaatctct
     9361 tcttttctaa tgttaatgaa taggataatc tcatttttag ctgctcgtga gagcagtctt
     9421 tttttgtttc agggtacgtc ctattctttg gatttccgcg ctattaaata accgatactt
     9481 ggggattggg gtagcgcccc aaaatacaaa aagaagaagt tgcataagcg gatttttctt
     9541 ttttttcgca
//
