LOCUS       NZ_LR135225             9292 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7040 plasmid 7.
ACCESSION   NZ_LR135225
VERSION     NZ_LR135225.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884924
            Assembly: GCF_900639385.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:57:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,106
            CDSs (total)                      :: 3,015
            Genes (coding)                    :: 2,884
            CDSs (with protein)               :: 2,884
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 59 of 131
            Pseudo Genes (incomplete)         :: 72 of 131
            Pseudo Genes (internal stop)      :: 34 of 131
            Pseudo Genes (multiple problems)  :: 27 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135225.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7040"
                     /db_xref="taxon:1352"
                     /plasmid="7"
     gene            <1..352
                     /locus_tag="EQB60_RS16160"
     CDS             <1..352
                     /locus_tag="EQB60_RS16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_127822571.1"
                     /translation="NQTVQYNFELAKKFNSNISNDHIYNLLTDFSLPKDILKEKIFLLS
                     GGEQQRIALIRNMLKPFDILFADEPTGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDL
                     NVLNYGTNIIEL"
                     /name="ATP-binding cassette domain-containing protein"
     gene            466..603
                     /locus_tag="EQB60_RS16425"
     CDS             466..603
                     /locus_tag="EQB60_RS16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            1045..1425
                     /locus_tag="EQB60_RS16165"
     CDS             1045..1425
                     /locus_tag="EQB60_RS16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_127822572.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1407..2321
                     /locus_tag="EQB60_RS16170"
     CDS             1407..2321
                     /locus_tag="EQB60_RS16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2281..2559
                     /locus_tag="EQB60_RS16830"
     CDS             2281..2559
                     /locus_tag="EQB60_RS16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            2561..3250
                     /locus_tag="EQB60_RS16180"
     CDS             2561..3250
                     /locus_tag="EQB60_RS16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127822569.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLLEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            4080..4817
                     /locus_tag="EQB60_RS16190"
     CDS             4080..4817
                     /locus_tag="EQB60_RS16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            4845..5381
                     /locus_tag="EQB60_RS16195"
     CDS             4845..5381
                     /locus_tag="EQB60_RS16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_127822573.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTPILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5397..5567
                     /locus_tag="EQB60_RS16430"
     CDS             5397..5567
                     /locus_tag="EQB60_RS16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(5879..6340)
                     /locus_tag="EQB60_RS16200"
     CDS             complement(5879..6340)
                     /locus_tag="EQB60_RS16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6473..6847
                     /locus_tag="EQB60_RS16205"
     CDS             6473..6847
                     /locus_tag="EQB60_RS16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            6901..9027
                     /locus_tag="EQB60_RS16210"
     CDS             6901..9027
                     /locus_tag="EQB60_RS16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            9024..>9292
                     /locus_tag="EQB60_RS16215"
     CDS             9024..>9292
                     /locus_tag="EQB60_RS16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_127822574.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALL"
                     /name="ATP-binding cassette domain-containing protein"
     BGC             1473..9292
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4081..4817
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1408..2318
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaatcaaaca gtacaataca atttcgaatt agcaaaaaag tttaacagta atatttccaa
       61 tgatcatatt tataatttat taacagattt ttctttgcca aaagatatct taaaagaaaa
      121 aatctttctc ttaagcggtg gagaacaaca aagaatagca ttaattagaa atatgttaaa
      181 accatttgat atattatttg cagatgaacc tacaggaaat ttagataaag aaaactcaac
      241 ttttataatg aactttctga aaaatttaac agaaaaagaa aaaaaagccg ttgtactagt
      301 aacacatgac ttaaatgttc taaactatgg aactaatatt atagaattat aatttttacc
      361 cgtgatgcta gctgatttaa aactagcacc acgggtattt attttatatt ccatctctcc
      421 tatcccaaaa tttaaataat cacttaataa aaaggaggca ccctagtgat tttattgaaa
      481 aaagagtatg aaaaatttta tgatagactt atttattctt tgcaattgcc aaaaaaatta
      541 aaccgtcaat tacaaaagac ttatgaaact aaatcaactg aagaatggga gagttattgc
      601 tagatttttg gtagaagtaa caaaaagtgg acatattatc ataatgactc gccgaaagtc
      661 tgataaaaaa ttggctaaaa atattgctga atgtttaata aaactatcag ttttagctaa
      721 atagttagta gtttacacaa aaaagcattt atgttttttg ttgcaaagta acaatttcat
      781 cttcggtgaa ataagcgaaa ctttacaaaa agtggctagc atttgctagc tttttttgta
      841 ttgtggagcg tggcttttga gggggattca agggggagct tgcgcccctt tgacaagcgg
      901 ttatttgcac caaatacgta ggtatttggc tagcaaatat agagcttgcc aaaccctttt
      961 agtgagcttt actcattgta cctttttagg tacaatggta ttgcgaacga tacgtccgta
     1021 ggacgtactg aaagggtggg acttatgagc gaacaaaatc aaaatttggc tagcaattct
     1081 tctaaaaaat atacctatcg atccgagcca aaacaaatca gttttcgagt gagcgaatcc
     1141 gaatttgcaa agctgaagca gtcagctgaa gctttgcaaa tgagtgtgcc ggctttcgtg
     1201 aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga cattgctcag
     1261 gcaatcgcgc gcgatttagc caaagctgga gggaatatca atcaaattgc taagtggtgc
     1321 aatacacacc aacacgacgt tgctcctgga gacgcccaac gcttatccga aaacctaaaa
     1381 atcatgcaaa aggagctaca aaagatatgg caacaattaa ggtaagccga accacttctt
     1441 gtagtcgtgc aattaattac gcagaaccac gagcaaccat taaaacagga ataaactgtg
     1501 acgtcgatta tgccaaaagt gaaatgaaac aaattcgcat gctgtatgga aaagacgacc
     1561 gtgtacaggc acacttattg attcaatctt ttcgaccagg agaaattacc gctgaaaaag
     1621 ctaatcaact aggggcagag tatgcaaaaa aaatagcccc cgatcaccaa acagctattt
     1681 acacacatac agacaaagac catattcata atcacattgt cattaactcc gttaatctgg
     1741 agactggaaa aaaatttcaa gctcatggtc aagcatttct aaacaaatgt tatgacctaa
     1801 atgacgagat ttgtcttgca cataacttac caatcactga gcgaggaaag aaaccagaaa
     1861 aacgaaccat gtctgaaata aaattaaaag aaaaaaatga acctgtttgg aaagacgaaa
     1921 ttcggattgc gatcgatcaa acaatgaaaa acaaaaaaac cagaacctat gaccaatttt
     1981 gtaattccct caaaacattg gggattcatt gcttcaatcg cgggaaaaat ttcacttacg
     2041 aattgatcca caaaaagaaa aaagtacgat ccaacagact aggaaaggac tacgaaaagg
     2101 agacgatttt acatgagctg gacagacgag aacgaaccta taaccaacag cgaaccaacg
     2161 agctacgaag agctcgagct agcattcgcg aaactgcaga agaagtacga agagaacaac
     2221 gaactcttac gcctgaacaa ccggaaatta gaccggctcg aagaaaccat gaacaacacg
     2281 ttgaaagagg aacagaccac caacaagaat tatcgctcta aaacgttaaa agacatagaa
     2341 aacgaaataa agaaacaacg tgcttttctc gttccttata cgcaagaaag gatacaaact
     2401 acgattgatt tacaagttaa aaaactccat caagagctat tagaccagcg aaataaggaa
     2461 cagaaaaagg tgcttattca acgatcattg gattttcttg cccccttatg ttgcttagtc
     2521 ctgacactgt tcgcattttg gttagtatgg ggaggctaaa atggcatttt atttacccta
     2581 tctactcatt tttgttagta tttcaggaag tatttggctc atctataaaa tttttcaaac
     2641 aagatattca ttaaaaggct ctaaaattcg atttaagcga tttttctttc ttggctgtat
     2701 attttccttg ataatcgttt caagcggctt attcggcgtt ttagagggaa ataagcgcgt
     2761 ttctagaagt attttattag gaaatgtcac tcaaaagtat gaatcagcgc gtgataaaaa
     2821 gaaaaaagag caagcattag cacaaaaaat ggaagagttt accacttgtt atgaggaaat
     2881 gaacgatatt tttgtgaatc aagaaaaacg tttgacggat aaaaacatgg aaaaactcac
     2941 tcgtctttat caaaacttgc tagaaaaaca gcaaaaagag gttcaagaca actatgagcg
     3001 aaccaaaaaa gacgtgcaat atgtaaaaga tacaaagata gaagaagcct gtagtgatct
     3061 atttggagac acaaaccctt ggtttgccag tgaagaagaa aaaaaagaaa aacaacaatc
     3121 tgtcacatat gaacgctatg aaatcttact tcaacaagca actaacatgc aaaatccgac
     3181 aaaaaaagaa accgccttaa actatctaaa aagtgtcaag gaatggttag atcagcaaca
     3241 acaaaattga atttgattta aaatcatgtt aaactgaatg tgcaaatgat caaacattag
     3301 atacacgaaa gccccagaga ggtcggattc tctggggctt tttttcaggt tgatgtcgcc
     3361 tttattgatt ttacttcttg tttcggttat tcaaccaata actgaataaa gagacacaaa
     3421 cttctataag aagggggcaa atgaacaatt caaacattag atacacctcc ttccagtcgc
     3481 aagactgtcg gataggcgac aagttattat atcttaaaaa attaaatttg tgtggtgttt
     3541 ttaagtatca cttgttttag ggttgttagg gcttgccctg accgcctgta aggcgcttct
     3601 cgactagatt aagggaaatt aatcaagaat tgtcttagaa cggcttagac aagctttcag
     3661 agtctcacac ctcctctttg aacaacggtt ttagcaaagg agccggaggc gactgcgcag
     3721 tataatatga gagcatagcg atcacacctt tttagcgacc gtagggagca agttttccag
     3781 tttagaaaaa ttcgcgaacc tttttggagg aaaaagtagg agttttttag gcgggaaatt
     3841 caatgtgctc ttaagatata ctgttagata tactgttaga tatactgtta ataataactg
     3901 ttagggatta gcaaccttat gggagagtaa gtattgacat aggtagtggg ggataaattg
     3961 tcacactatg ggggataaat tgtcacacta tgggggataa attgtcacac tatgggggat
     4021 aaattgtgtt ttacatcctc ttatgtctat gataacataa tttattgagg tgatttttat
     4081 tgagtaacga tatagttcga tataataatg gattaaattc tgtcccttta agaaaattta
     4141 cccctataga tatggattta ttttggggaa tatgttctaa aatgaaaaga aaaggtacaa
     4201 aagaagaaat ttttacattt aaacaaataa aagaattaat tgattacaat ataaatgcac
     4261 gtggcgaccg ttttgctaat gatttaataa aaatgtcaaa aaaaatcatt actttgtctt
     4321 ttatttatga agacgaaaaa acatatgaac aattaaatct gtttcaaaaa tttaaaatcg
     4381 ataaagtgaa tgaaacatta ttaataaagg tttctgaaga atttgaatat atacttaatt
     4441 caatcggaac taattttact cgtttcgaat tagaaaatat gacaaactta aagagtagct
     4501 acgttaaaga gttatatcgc caacttatgg ctcataaaga cagaagatct aaaaaaggtc
     4561 actggtttgt aaaaatagaa gattttagag ctctattagc aataccagaa agttatcgca
     4621 tgagagatat tgataaacaa atttttgccc aagcgaaaaa agaattttta aacgttaatt
     4681 caactcaccc tgctattttt aaaacttttg aaatcaaaaa aattaaagcc tacaaaggaa
     4741 ataagatagg atctctgcaa atttattttg aagaaaatat accttcaatt accttagata
     4801 attggttaga gaaataatac ctaacaattt aggaggcaat tatcatgcaa cccaaagaaa
     4861 cctttacaat aaaggaatta tctgaattat ttgaaataag taggcaagca atctctaaac
     4921 atgttgataa attagacccg tccttattag ctaaaaatga gcgtggttat aaggttgtat
     4981 tacgttctgg agtattgcaa cttgctagca acttaggtaa ccaaaggtta ctatataggt
     5041 tgcatgagta tgaacaagaa aacgataaaa acgtttatat atcaagcaaa aacgtgattg
     5101 taaacgagtt gctagataag ttgcgtgaag aaaatgaatt tttacgagaa caactttccc
     5161 tcaaagagca aaataattct aatatgcaaa aattactcga ccagcaacaa cagctcaccc
     5221 tacaggcaaa tcaacagatc gaaaaactgc aagaacagtt acaattagct tatacggaag
     5281 aaactccaga agatgaatca actcctattc tttctgaaga aacagaatcg atagaaaaac
     5341 acacaaaaaa gaagcaaaaa tggtggcaac tttggaaata actggaggga acattcatga
     5401 caagaaaaaa tcttctattc ggttctattt tactaggaat tctaggattc agtccgatgt
     5461 tattgtcagt gatcaataaa gggaataaac atggtgaatt gaaattgaat ccaaattatt
     5521 acaacagtcc taacgaagaa ttgtttgtaa aagaaacgga taaataataa aaaagactgc
     5581 tcttccgagc agccagttaa aaaataatga gtgtttattc ttgaaaaagc acgcgcttat
     5641 gaagcgctac caaaaattta ccatatttta caaaaaataa caagtcttaa aaaaatgaca
     5701 gataaaaatg cacaaagaat tagatataga taaccgcgaa aaatcaagct ttagaaagtt
     5761 gcttttagct agaccaatta aaaatcacaa agtgagcgtc tctatccatt ggtagagggg
     5821 ctacttggca tagtcgacat tatgtcaact atgcaattaa ataactaact acttcatatt
     5881 agcttaataa aataatcaaa ataactaggg ggaaaaacat tgtataaaaa aatttttcat
     5941 tttttaaata agaatagtaa atataaaaat aatttaagat atctattact attaatccag
     6001 atagagcaaa ccaagtaaat attccatgtg taaagctaaa aaaattcatt tgaatcaatt
     6061 caaataaacc taatatagta acaaatatat taaaaataat agctgtcaat accagtaaat
     6121 tatttttttt attttttgaa ttatcttcat ttaaaataga atttatgtta gtactataaa
     6181 attcacaaat tttttttagt gtctctatat ctggtgttgt tttgttattt tcccactttg
     6241 aaatagtttg acgtgtaacg ttaaattttc ttgccacata ttcttgagta tacccttttt
     6301 tcactcttaa ttctcttagc ttagatccta aatcctccat tttttctcct atgtaaacga
     6361 ttatttacat aaaattttat cataaaaata ctaacgcgcc aaaaaagttc tttattttat
     6421 aaaacacact aaaataaaat aattctagta aaaaagatag gagaaataag ttatgaaaaa
     6481 atttttatgt ctttctgctt taatgggtgt cttattaact agtggaggaa tagctagtgc
     6541 cacagaagct ttaaatctag atgtacccga agaccatacc gccatttacg gaggaggaat
     6601 ggaagctaca gaagaaggat tttcttctag taaattgaga tatgctgctg gcggcgggga
     6661 tttcaactgt ggggttaatg gatttaaagt ttatgcaaat tactaccatg ctagagctaa
     6721 acatagtgca acagctaaaa atggacgagg cggtcaagta cgatcagttc agaaagctgg
     6781 agtaagagct tatgcaacat gcaatgccac acttactgga aatactggtt ggtggaatgt
     6841 ttattaaaaa agagagttta aactctcttt tttatttaca tttgaaagga aattcaaaat
     6901 atgttaaaaa aaataaacgc tgttttatca ctttttgtct gcattacaat actatttttt
     6961 tctactaatg ttataaatgg tataaagact tctgaagcgc tatataaaaa taccgctgaa
     7021 attcatatgc aatataaaga tgatatggac tttaacagtt atataaacgc tttgtatgaa
     7081 atatctacac aagaaaatat taatatttct caatatagtt ttactgctgt taaccaacta
     7141 actattcttg ctacaaaccc taacgctaac aaacactgga aattttctaa aaaaaatata
     7201 ttagcaagaa acgattctaa agtccattat actaataaca aaaattctaa gtaccaatta
     7261 aaacttccaa ataatttttt aaatataaat atttatcctt tttctcatgt ggaaaatatt
     7321 ggattaagtg aaatattata tgtacaagga aactcaaaca aattaattcc tgtactgaaa
     7381 aaatatggaa cacctactat ttccaatatc aatcaatcag atacttttca aattaatact
     7441 aatattgttc ttgttatctg ctatcttagt atatttattg taattactac aattatattt
     7501 gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt taggctataa ctcttttgat
     7561 actataaaag attctattaa agagttaaac ctcttatttt gttctttgat tataactttc
     7621 attttatcta tttttttgag catatattac agtaacgaaa attttttcct atcatttctt
     7681 ctatgtagta tttttcattt tataattagt tttttattta tcatagttac tctaagtatc
     7741 tatcatttat ctagtataaa aaattatcta aaaaattcac gaccacttaa gttaaatctt
     7801 tgtatattaa atatctgtct attcttaagc atgatccttc tgttaatagc ttctacaaaa
     7861 gtaataaata cccataatga agccgaaaat aattctctta aatattggga aagaactact
     7921 aatttataca aaacaaatat tactaatcaa ttaaatagaa acaacaccgt agaagaaaat
     7981 aactacttga aaaaagctag taaatttgtt tataaaatac agaagaatta taaaactttt
     8041 attatcgcgc catacaatta cgcaactatc caagagaata ataaagaatt ttttattgga
     8101 caaaaagttt atccaaattt tgaagactat gttagtcaac ctgctggtgc aggaatatgt
     8161 gttgatttaa actatctaaa atattacaat cctatttctt ttgaagaatc tactgactta
     8221 aatttaataa actctgatcc actaactact tacattctag tacctaaaaa atataaagaa
     8281 tatgcaaaaa tgattgaaaa aaattattta gaagacatac agtttagact tgaggactct
     8341 cctcctcaag ctccttataa aaatcctaat ttgaaaaatt taaaaataaa actaatattt
     8401 gttaataata accaaaagta ttttagtttt aataccttat atggaaaggc aaaagataac
     8461 tatacaataa ctgatcccat agttagagtt attaatccag aaataacaga atcattattt
     8521 tggggaaata tattgactag tgatggtgga ttattttttg atcagaaaca tacttcaaac
     8581 caaaactttt ttaatcaaat taatccgtat ataaaagaat ttaacctaga aaatatcata
     8641 aatttttctt attctgtttt tcaagaaact ggtgaattat tatctcaaaa aaaagtaaat
     8701 aaattaaatg catatctaca atatctttta atattaggat tatttataat aattaatttt
     8761 caaatgattt ttgttagttt tactataaat tcaaaaaaaa tagctttaaa aaaaatattt
     8821 ggatattcac caataagtca agttttagat ttatgtttac tcccttttac aatagagtta
     8881 tttgctacta tgtgttttca attgatattt tcaaaaaaca cttacttgta ttttataatt
     8941 tttatgatct ttttaataaa tctaattact tctattatta tttataaaaa aaatagtttt
     9001 aaatatttta aggagaatat tttatgatat tatcagttga gaatttcacc ctgtcaatta
     9061 ataaaaaaaa gatcaaacaa aattggaact tttcaatatc agaaaaagat tttattatag
     9121 taacaggaaa aagtggtagt ggaaaaacta ctctgttaaa taatttaagc ctaatagata
     9181 aacaatatac cggtaactta ttctattttg aaaagaaagt ttctaaacat gatatacaac
     9241 gtctacgaag aaatcatatt agctatttat ttcaaaacta tgctcttctt ga
//
