LOCUS       NZ_LR135208             9317 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E7171 plasmid 6.
ACCESSION   NZ_LR135208
VERSION     NZ_LR135208.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4885012
            Assembly: GCF_900639365.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9317)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:22:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,172
            CDSs (total)                      :: 3,080
            Genes (coding)                    :: 2,948
            CDSs (with protein)               :: 2,948
            Genes (RNA)                       :: 92
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 47 of 132
            Pseudo Genes (incomplete)         :: 84 of 132
            Pseudo Genes (internal stop)      :: 28 of 132
            Pseudo Genes (multiple problems)  :: 24 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135208.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E7171"
                     /db_xref="taxon:1352"
                     /plasmid="6"
     gene            complement(9..2135)
                     /locus_tag="EQB62_RS16520"
     CDS             complement(9..2135)
                     /locus_tag="EQB62_RS16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            complement(2189..2563)
                     /locus_tag="EQB62_RS16525"
     CDS             complement(2189..2563)
                     /locus_tag="EQB62_RS16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            2696..3157
                     /locus_tag="EQB62_RS16530"
     CDS             2696..3157
                     /locus_tag="EQB62_RS16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3469..3639)
                     /locus_tag="EQB62_RS16765"
     CDS             complement(3469..3639)
                     /locus_tag="EQB62_RS16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3655..4194)
                     /locus_tag="EQB62_RS16535"
     CDS             complement(3655..4194)
                     /locus_tag="EQB62_RS16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4222..4959)
                     /locus_tag="EQB62_RS16540"
     CDS             complement(4222..4959)
                     /locus_tag="EQB62_RS16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            5604..5690
                     /locus_tag="EQB62_RS16545"
     CDS             5604..5690
                     /locus_tag="EQB62_RS16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(5811..6500)
                     /locus_tag="EQB62_RS16550"
     CDS             complement(5811..6500)
                     /locus_tag="EQB62_RS16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127824149.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYEQTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6502..6780)
                     /locus_tag="EQB62_RS17120"
     CDS             complement(6502..6780)
                     /locus_tag="EQB62_RS17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            complement(6740..7654)
                     /locus_tag="EQB62_RS16560"
     CDS             complement(6740..7654)
                     /locus_tag="EQB62_RS16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7636..8016)
                     /gene="mobC"
                     /locus_tag="EQB62_RS16565"
     CDS             complement(7636..8016)
                     /gene="mobC"
                     /locus_tag="EQB62_RS16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            complement(8458..8595)
                     /locus_tag="EQB62_RS16770"
     CDS             complement(8458..8595)
                     /locus_tag="EQB62_RS16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            complement(8709..>9317)
                     /locus_tag="EQB62_RS16570"
     CDS             complement(8709..>9317)
                     /locus_tag="EQB62_RS16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_232044179.1"
                     /translation="VENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLLNNLS
                     LIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKFNSNI
                     SNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGNLDKE
                     NSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     BGC             1..7563
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4223..4959)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6744..7654)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgataatatc ataaaatatt ctccttaaaa tatttaaaac tatttttttt ataaataata
       61 atagaagtaa ttagatttat taaaaagatc ataaaaatta taaaatacaa gtaagtgttt
      121 tttgaaaata tcaattgaaa acacatagta gcaaataact ctattgtaaa agggagtaaa
      181 cataaatcta aaacttgact tattggtgaa tatccaaata ttttttttaa agctattttt
      241 tttgaattta tagtaaaact aacaaaaatc atttgaaaat taattattat aaataatcct
      301 aatattaaaa gatattgtag atatgcattt aatttattta cttttttttg agataataat
      361 tcaccagttt cttgaaaaac agaataagaa aaatttatga tattttctag gttaaattct
      421 tttatatacg gattaatttg attaaaaaag ttttggtttg aagtatgttt ctgatcaaaa
      481 aataatccac catcactagt caatatattt ccccaaaata atgattctgt tatttctgga
      541 ttaataactc taactatggg atcagttatt gtatagttat cttttgcctt tccatataag
      601 gtattaaaac taaaatactt ttggttatta ttaacaaata ttagttttat ttttaaattt
      661 ttcaaattag gatttttata aggagcttga ggaggagagt cctcaagtct aaactgtatg
      721 tcttctaaat aatttttttc aatcattttt gcatattctt tatatttttt aggtactaga
      781 atgtaagtag ttagtggatc agagtttatt aaatttaagt cagtagattc ttcaaaagaa
      841 ataggattgt aatattttag atagtttaaa tcaacacata ttcctgcacc agcaggttga
      901 ctaacatagt cttcaaaatt tggataaact ttttgtccaa taaaaaattc tttattattc
      961 tcttggatag ttgcgtaatt gtatggcgcg ataataaaag ttttataatt cttctgtatt
     1021 ttataaacaa atttactagc ttttttcaag tagttatttt cttctacggt gttgtttcta
     1081 tttaattgat tagtaatatt tgttttgtat aaattagtag ttctttccca atatttaaga
     1141 gaattatttt cggcttcatt atgggtattt attacttttg tagaagctat taacagaagg
     1201 atcatgctta agaatagaca gatatttaat atacaaagat ttaacttaag tggtcgtgaa
     1261 ttttttagat aattttttat actagataaa tgatagatac ttagagtaac tatgataaat
     1321 aaaaaactaa ttataaaatg aaaaatacta catagaagaa atgataggaa aaaattttcg
     1381 ttactgtaat atatgctcaa aaaaatagat aaaatgaaag ttataatcaa agaacaaaat
     1441 aagaggttta actctttaat agaatctttt atagtatcaa aagagttata gcctaacatt
     1501 ttttttagag tgatttcttt catttttgaa aaagcaaata taattgtagt aattacaata
     1561 aatatactaa gatagcagat aacaagaaca atattagtat taatttgaaa agtatctgat
     1621 tgattgatat tggaaatagt aggtgttcca tattttttca gtacaggaat taatttgttt
     1681 gagtttcctt gtacatataa tatttcactt aatccaatat tttccacatg agaaaaagga
     1741 taaatattta tatttaaaaa attatttgga agttttaatt ggtacttaga atttttgtta
     1801 ttagtataat ggactttaga atcgtttctt gctaatatat tttttttaga aaatttccag
     1861 tgtttgttag cgttagggtt tgtagcaaga atagttagtt ggttaacagc agtaaaacta
     1921 tattgagaaa tattaatatt ttcttgtgta gatatttcat acaaagcgtt tatataactg
     1981 ttaaagtcca tatcatcttt atattgcata tgaatttcag cggtattttt atatagcgct
     2041 tcagaagtct ttataccatt tataacatta gtagaaaaaa atagtattgt aatgcagaca
     2101 aaaagtgata aaacagcgtt tatttttttt aacatatttt gaatttcctt tcaaatgtaa
     2161 ataaaaaaga gagtttaaac tctctttttt aataaacatt ccaccaacca gtatttccag
     2221 taagtgtggc attgcatgtt gcataagctc ttactccagc tttctgaact gatcgtactt
     2281 gaccgcctcg tccattttta gctgttgcac tatgtttagc tctagcatgg tagtaatttg
     2341 cataaacttt aaatccatta accccacagt tgaaatcccc gccgccagca gcatatctca
     2401 atttactaga agaaaatcct tcttctgtag cttccattcc tcctccgtaa atggcggtat
     2461 ggtcttcggg tacatctaga tttaaagctt ctgtggcact agctattcct ccactagtta
     2521 ataagacacc cattaaagca gaaagacata aaaatttttt cataacttat ttctcctatc
     2581 ttttttacta gaattatttt attttagtgt gttttataaa ataaagaact tttttggcgc
     2641 gttagtattt ttatgataaa attttatgta aataatcgtt tacataggag aaaaaatgga
     2701 ggatttagga tctaagctaa gagaattaag agtgaaaaaa gggtatactc aagaatatgt
     2761 ggcaagaaaa tttaacgtta cacgtcaaac tatttcaaag tgggaaaata acaaaacaac
     2821 accagatata gagacactaa aaaaaatttg tgaattttat agtactaaca taaattctat
     2881 tttaaatgaa gataattcaa aaaataaaaa aaataattta ctggtattga cagctattat
     2941 ttttaatata tttgttacta tattaggttt atttgaattg attcaaatga atttttttag
     3001 ctttacacat ggaatattta cttggtttgc tctatctgga ttaatagtaa tagatatctt
     3061 aaattatttt tatatttact attcttattt aaaaaatgaa aaattttttt atacaatgtt
     3121 tttcccccta gttattttga ttattttatt aagctaatat gaagtagtta gttatttaat
     3181 tgcatagttg acataatgtc gactatgcca agtagcccct ctaccaatgg atagagacgc
     3241 tcactttgtg atttttaatt ggtctagcta aaagcaactt tctaaagctt gatttttcgc
     3301 ggttatctat atctaattct ttgtgcattt ttatctgtca tttttttaag acttgttatt
     3361 ttttgtaaaa tatggtaaat ttttggtagc gcttcataag cgcgtgcttt ttcaagaata
     3421 aacactcatt attttttaac tggctgctcg gaagagcagt cttttttatt atttatccgt
     3481 ctcttttaca aacaattctt cgttaggact gttgtaataa tttggattca atttcaattc
     3541 accatgttta ttccctttat tgattactga caataacatc ggactgaagc ctagaattcc
     3601 tagtaaaata gaaccgaata gaagattttt tcttgtcatg aatgttccct ccagttattt
     3661 ccaaagttgc caccattttt gcttcttctt ttttgtgtgt ttttctatcg attctgtttc
     3721 ttcagaaaga atagtagttg attcatcttc tggagtttct tccgtataag ctaattgtaa
     3781 ctgttcttgc agtttttcga tctgttgatt tgcctgtagg gtgagctgtt gttgctggtc
     3841 gagtaatttt tgcatattag aattattttg ctctttgagg gaaagttgtt ctcgtaaaaa
     3901 ttcattttct tcacgcaact tatctagcaa ctcgtttaca atcacgtttt tgcttgatat
     3961 ataaacgttt ttatcgtttt cttgttcata ctcatgcaac ctatatagta acctttggtt
     4021 acctaagttg ctagcaagtt gcaatactcc agaacgtaat acaaccttat aaccacgctc
     4081 atttttagct aataaggacg ggtctaattt atcaacatgt ttagagattg cttgcctact
     4141 tatttcaaat aattcagata attcctttat tgtaaaggtt tctttgggtt gcatgataat
     4201 tgcctcctaa attgttaggt attatttctc taaccaatta tctaaggtaa ttgaaggtat
     4261 attttcttca aaataaattt gcagagatcc tatcttattt cctttgtagg ctttaatttt
     4321 tttgatttca aaagttttaa aaatagcagg gtgagttgaa ttaacgttta aaaattcttt
     4381 tttcgcttgg gcaaaaattt gtttatcaat atctctcatg cgataacttt ctggtattgc
     4441 taatagagct ctaaaatctt ctatttttac aaaccagtga ccttttttag atcttctgtc
     4501 tttatgagcc ataagttggc gatataactc tttaacgtag ctactcttta agtttgtcat
     4561 attttctaat tcgaaacgag taaaattagt tccgattgaa ttaagtatat attcaaattc
     4621 ttcagaaacc tttattaata atgtttcatt cactttatcg attttaaatt tttgaaacag
     4681 atttaattgt tcatatgttt tttcgtcttc ataaataaaa gacaaagtaa tgattttttt
     4741 tgacattttt attaaatcat tagcaaaacg gtcgccacgt gcatttatat tgtaatcaat
     4801 taattctttt atttgtttaa atgtaaaaat ttcttctttt gtaccttttc ttttcatttt
     4861 agaacatatt ccccaaaata aatccatatc tataggggta aattttctta aagggacaga
     4921 atttaatcca ttattatatc gaactatatc gttactcaat aaaaatcacc tcaataaatt
     4981 atgttatcat agacataaga ggatgtaaaa cacaatttat cccccatagt gtgacaattt
     5041 atcccccata gtgtgacaat ttatccccca tagtgtgaca atttatcccc catagtgtga
     5101 caatttatcc cccactacct atgtcaatac ttactctccc ataaggttgc taatccctaa
     5161 cagttattat taacagtata tctaacagta tatctaacag tatatcttaa gagcacattg
     5221 aatttcccgc ctaaaaaact cctacttttt cctccaaaaa ggttcgcgaa tttttctaaa
     5281 ctggaaaact tgctccctac ggtcgctaaa aaggtgtgat cgctatgctc tcatattata
     5341 ctgcgcagtc gcctccggct cctttgctaa aaccgttgtt caaagaggag gtgtgagact
     5401 ctgaaagctt gtctaagccg ttctaagaca attcttgatt aatttccctt aatctagtcg
     5461 agaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaaacaag tgatacttaa
     5521 aaacaccaca caaatttaat tttttaagat ataataactt gtcgcctatc cgacagtctt
     5581 gcgactggaa ggaggtgtat ctaatgtttg aactgttcat ttgccccctt cttgtaggag
     5641 tttgtgtctc tttattcagt tattggttga ataaccgaaa caagaagtaa aatcaataaa
     5701 ggcgacatca acctgaaaaa aagccccaga gaatccgacc tctctggggc tttcgtgtat
     5761 ctaatgtttg atcatttgca cattcagttt aacatgattt taaatcaaat tcaattttgt
     5821 tgttgctgat ctaaccattc cttgacactt tttagatagt ttaaggcggt ttcttttttt
     5881 gtcggatttt gcatgttagt tgcttgttga agtaagattt catagcgttc atatgtgaca
     5941 gattgttgtt tttctttttt ttcttcttca ctggcaaacc aagggtttgt gtctccaaat
     6001 agatcactac aggcttcttc tatctttgta tcttttacat attgcacgtc ttttttggtt
     6061 tgctcatagt tgtcttgaac ctctttttgc tgtttttctg gcaagttttg ataaagacga
     6121 gtgagttttt ccatgttttt atccgtcaaa cgtttttctt gattcacaaa aatatcgttc
     6181 atttcctcat aacaagtggt aaactcttcc attttttgtg ctaatgcttg ctcttttttc
     6241 tttttattac gtgctgattc atacttttga gtgacatttc ctaataaaat acttctagaa
     6301 acgcgcttat tgccctctaa aacgcctaat aagccgcttg aaacgattat caaggaaaat
     6361 atacagccaa gcaagaaaaa tcgcttaaat cgaattttag agccttttaa tgaatatctt
     6421 gtttggaaaa ttttatagat gagccaaata cttcctgaaa tactaacaaa aatgagtaga
     6481 tagggtaaat aaaatgccat tttagcctcc ccatactaac caaaatgcga acagtgtcag
     6541 gactaagcaa cataaggggg caagaaaatc caatgatcgt tgaataagca cctttttctg
     6601 ttccttattt cgctggtcta atagctcttg atggagtttt ttaacttgta aatcaatcgt
     6661 agtttgtatc ctttcttgcg tataaggaac gagaaaagca cgttgtttct ttatttcgtt
     6721 ttctatgtct tttaacgttt tagagcgata attcttgttg gtggtctgtt cctctttcaa
     6781 cgtgttgttc atggtttctt cgagccggtc taatttccgg ttgttcaggc gtaagagttc
     6841 gttgttctct tcgtacttct tctgcagttt cgcgaatgct agctcgagct cttcgtagct
     6901 cgttggttcg ctgttggtta taggttcgtt ctcgtctgtc cagctcatgt aaaatcgtct
     6961 ccttttcgta gtcctttcct agtctgttgg atcgtacttt tttctttttg tggatcaatt
     7021 cgtaagtgaa atttttcccg cgattgaagc aatgaatccc caatgttttg agggaattac
     7081 aaaattggtc ataggttctg gtttttttgt ttttcattgt ttgatcgatc gcaatccgaa
     7141 tttcgtcttt ccaaacaggt tcattttttt cttttaattt tatttcagac atggttcgtt
     7201 tttctggttt ctttcctcgc tcagtgattg gtaagttatg tgcaagacaa atctcgtcat
     7261 ttaggtcata acatttgttt agaaatgctt gaccatgagc ttgaaatttt tttccagtct
     7321 ccagattaac ggagttaatg acaatgtgat tatgaatatg gtctttgtct gtatgtgtgt
     7381 aaatagctgt ttggtgatcg ggggctattt tttttgcata ctctgcccct agttgattag
     7441 ctttttcagc ggtaatttct cctggtcgaa aagattgaat caataagtgt gcctgtacac
     7501 ggtcgtcttt tccatacagc atgcgaattt gtttcatttc acttttggca taatcgacgt
     7561 cacagtttat tcctgtttta atggttgctc gtggttctgc gtaattaatt gcacgactac
     7621 aagaagtggt tcggcttacc ttaattgttg ccatatcttt tgtagctcct tttgcatgat
     7681 ttttaggttt tcggataaac gttgagcgtc accagaagca acgtcatgtt ggtgtgtatt
     7741 gcaccactta gcaatttgat tgatattccc tccagctttg gctaaatcgc gcgcgattgc
     7801 ctgggcaatg tctggcgcca ctttcggtgt caccagacgc gcgttttgtg ccttggcttt
     7861 cacgaaagcc ggcacactca tttgcaaagc ttcagccgat tgcttcagct ttgcaaattc
     7921 ggattcgctc actcgaaaac tgatttgttt tggctcggat cgataggtat attttttaga
     7981 agaattgcta gccaaatttt gattttgttc gctcataagt cccacccttt cagtacgtcc
     8041 tacggacgta tcgttcgcaa taccattgta cctaaaaagg tacaatgagt aaagctcact
     8101 aaaagggttt ggcaagctct atatttgcta gccaaatacc tacgtattta gtgcaaataa
     8161 ccgcttgtca aaggggcgca agctccccct tgaatccccc tcaaaagcca cgctccacaa
     8221 tacaaaaaaa gctagcaaat gctagccact ttttgtaaag tttcgcttat ttcaccgaag
     8281 atgaaattgt tactttgcaa caaaaaacat aaatgctttt ttgtgtaaac tactaactat
     8341 ttagctaaaa ctgatagttt tattaaacat tcagcaatat ttttagccaa ttttttatca
     8401 gactttcggc gagtcattat gataatatgt ccactttttg ttacttctac caaaaatcta
     8461 gcaataactc tcccattctt cagttgattt agtttcataa gtcttttgta attgacggtt
     8521 taattttttt ggcaattgca aagaataaat aagtctatca taaaattttt catactcttt
     8581 tttcaataaa atcactaggg tgcctccttt ttattaagtg attatttaaa ttttgggata
     8641 ggagagatgg aatataaaat aaatacccgt ggtgctagtt ttaaatcagc tagcatcacg
     8701 ggtaaaaatt ataattctat aatattagtt ccatagttta gaacatttaa gtcatgtgtt
     8761 actagtacaa cggctttttt ttctttttct gttaaatttt tcagaaagtt cattataaaa
     8821 gttgagtttt ctttatctaa atttcctgta ggttcatctg caaataatat atcaaatggt
     8881 tttaacatat ttctaattaa tgctattctt tgttgttctc caccgcttaa gagaaagatt
     8941 ttttctttta agatatcttt tggcaaagaa aaatctgtta ataaattata aatatgatca
     9001 ttggaaatat tactgttaaa cttttttgct aattcgaaat tgtattgtac tgtttgattt
     9061 tcaagaagag catagttttg aaataaatag ctaatatgat ttcttcgtag acgttgtata
     9121 tcatgtttag aaactttctt ttcaaaatag aataagttac cggtatattg tttatctatt
     9181 aggcttaaat tatttaacag agtagttttt ccactaccac tttttcctgt tactataata
     9241 aaatcttttt ctgatattga aaagttccaa ttttgtttga tctttttttt attaattgac
     9301 agggtgaaat tctcaac
//
