LOCUS       NZ_LR135201             9318 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6055 plasmid 5.
ACCESSION   NZ_LR135201
VERSION     NZ_LR135201.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884801
            Assembly: GCF_900639345.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9318)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:21:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,007
            CDSs (total)                      :: 2,916
            Genes (coding)                    :: 2,797
            CDSs (with protein)               :: 2,797
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 47 of 119
            Pseudo Genes (incomplete)         :: 71 of 119
            Pseudo Genes (internal stop)      :: 26 of 119
            Pseudo Genes (multiple problems)  :: 22 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6055"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            126..746
                     /locus_tag="EQB61_RS15695"
     CDS             126..746
                     /locus_tag="EQB61_RS15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            860..997
                     /locus_tag="EQB61_RS15930"
     CDS             860..997
                     /locus_tag="EQB61_RS15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            1439..1819
                     /gene="mobC"
                     /locus_tag="EQB61_RS15700"
     CDS             1439..1819
                     /gene="mobC"
                     /locus_tag="EQB61_RS15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            1801..2715
                     /locus_tag="EQB61_RS15705"
     CDS             1801..2715
                     /locus_tag="EQB61_RS15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002340563.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2675..2953
                     /locus_tag="EQB61_RS16260"
     CDS             2675..2953
                     /locus_tag="EQB61_RS16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            2955..3644
                     /locus_tag="EQB61_RS15715"
     CDS             2955..3644
                     /locus_tag="EQB61_RS15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3765..3851)
                     /locus_tag="EQB61_RS15720"
     CDS             complement(3765..3851)
                     /locus_tag="EQB61_RS15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110300541.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            4497..5234
                     /locus_tag="EQB61_RS15725"
     CDS             4497..5234
                     /locus_tag="EQB61_RS15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            5262..5801
                     /locus_tag="EQB61_RS15730"
     CDS             5262..5801
                     /locus_tag="EQB61_RS15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            5817..5987
                     /locus_tag="EQB61_RS15935"
     CDS             5817..5987
                     /locus_tag="EQB61_RS15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(6299..6760)
                     /locus_tag="EQB61_RS15735"
     CDS             complement(6299..6760)
                     /locus_tag="EQB61_RS15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6893..7267
                     /locus_tag="EQB61_RS15740"
     CDS             6893..7267
                     /locus_tag="EQB61_RS15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_002305242.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKAGV
                     RAYATCNATLTGNTGWWNVY"
                     /name="lactococcin 972 family bacteriocin"
     gene            7321..>9318
                     /locus_tag="EQB61_RS15745"
     CDS             7321..>9318
                     /locus_tag="EQB61_RS15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127836275.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCF"
                     /name="hypothetical protein"
     BGC             1893..9318
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4498..5234
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1802..2712
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.342"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caattgatat tttcaaaaaa cacttacttg tattttataa tttttatgat ctttttaata
       61 aatctaatta cttctattat tatttataaa aaaaatagtt ttaaatattt taaggagaat
      121 attttatgat attatcagtt gagaatttca ccctgtcaat taataaaaaa aagatcaaac
      181 aaaattggaa cttttcaata tcagaaaaag attttattat agtaacagga aaaagtggta
      241 gtggaaaaac tactctgtta aataatttaa gcctaataga taaacaatat accggtaact
      301 tattctattt tgaaaagaaa gtttctaaac atgatataca acgtctacga agaaatcata
      361 ttagctattt atttcaaaac tatgctcttc ttgaaaatca aacagtacaa tacaatttcg
      421 aattagcaaa aaagtttaac agtaatattt ccaatgatca tatttataat ttattaacag
      481 atttttcttt gccaaaagat atcttaaaag aaaaaatctt tctcttaagc ggtggagaac
      541 aacaaagaat agcattaatt agaaatatgt taaaaccatt tgatatatta tttgcagatg
      601 aacctacagg aaatttagat aaagaaaact caacttttat aatgaacttt ctgaaaaatt
      661 taacagaaaa agaaaaaaaa gccgttgtac tagtaacaca tgacttaaat gttctaaact
      721 atggaactaa tattatagaa ttataatttt tacccgtgat gctagctgat ttaaaactag
      781 caccacgggt atttatttta tattccatct ctcctatccc aaaatttaaa taatcactta
      841 ataaaaagga ggcaccctag tgattttatt gaaaaaagag tatgaaaaat tttatgatag
      901 acttatttat tctttgcaat tgccaaaaaa attaaaccgt caattacaaa agacttatga
      961 aactaaatca actgaagaat gggagagtta ttgctagatt tttggtagaa gtaacaaaaa
     1021 gtggacatat tatcataatg actcgccgaa agtctgataa aaaattggct aaaaatattg
     1081 ctgaatgttt aataaaacta tcagttttag ctaaatagtt agtagtttac acaaaaaagc
     1141 atttatgttt tttgttgcaa agtaacaatt tcatcttcgg tgaaataagc gaaactttac
     1201 aaaaagtggc tagcatttgc tagctttttt tgtattgtgg agcgtggctt ttgaggggga
     1261 ttcaaggggg agcttgcgcc cctttgacaa gcggttattt gcactaaata cgtaggtatt
     1321 tggctagcaa atatagagct tgccaaaccc ttttagtgag ctttactcat tgtacctttt
     1381 taggtacaat ggtattgcga acgatacgtc cgtaggacgt actgaaaggg tgggacttat
     1441 gagcgaacaa aatcaaaatt tggctagcaa ttcttctaaa aaatatacct atcgatccga
     1501 gccaaaacaa atcagttttc gagtgagcga atccgaattt gcaaagctga agcaatcggc
     1561 tgaagctttg caaatgagtg tgccggcttt cgtgaaagcc aaggcacaaa acgcgcgtct
     1621 ggtgacaccg aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc
     1681 tggagggaat atcaatcaaa ttgctaagtg gtgcaataca caccaacatg acgttgcttc
     1741 tggtgacgct caacgtttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat
     1801 atggcaacaa ttaaggtaag ccgaaccact tcttgtagtc gtgcaattaa ttacgcagaa
     1861 ccacgagcaa ccattaaaac aggaataaac tgtgacgtcg attatgccaa aagtgaaatg
     1921 aaacaaattc gcatgctgta tggaaaagac gaccgtgtac aggcacactt attgattcaa
     1981 tcttttcgac caggagaaat taccgctgaa aaagctaatc aactaggggc agagtatgca
     2041 aaaaaaatag cccccgatca ccaaacagct atttacacac atacagacaa agaccatatt
     2101 cataatcaca ttgtcattaa ctccgttaat ctggagactg gaaaaaaatt tcaagctcat
     2161 ggtcaagcat ttctaaacaa atgttatgac ctaaatgacg agatttgtct tgcacataac
     2221 ttaccaatca ctgagcgagg aaagaaacca gaaaaacgaa ccatgtctga aataaaatta
     2281 aaagaaaaaa atgaacctgt ttggaaagac gaaattcgga ttgcgatcga tcaaacaatg
     2341 aaaaacaaaa aaaccagaac ctatgaccaa ttttgtaatt ccctcaaaac attggggatt
     2401 cattgcttca atcgcgggaa aaatttcact tacgaattga tccacaaaaa gaaaaaagta
     2461 cgatccaaca gactaggaaa ggactacgaa aaggagacga ttttacatga gctggacaga
     2521 cgagaacgaa cctataacca acagcgaacc aacgagctac gaagagctcg agctagcatt
     2581 cgcgaaactg cagaagaagt acgaagagaa caacgaactc ttacgcctga acaaccggaa
     2641 attagaccgg ctcgaagaaa ccatgaacaa cacgttgaaa gaggaacaga ccaccaacaa
     2701 gaattatcgc tctaaaacgt taaaagacat agaaaacgaa ataaagaaac aacgtgcttt
     2761 tctcgttcct tatacgcaag aaaggataca aactacgatt gatttacaag ttaaaaaact
     2821 ccatcaagag ctattagacc agcgaaataa ggaacagaaa aaggtgctta ttcaacgatc
     2881 attggatttt cttgccccct tatgttgctt agtcctgaca ctgttcgcat tttggttagt
     2941 atggggaggc taaaatggca ttttatttac cctatctact catttttgtt agtatttcag
     3001 gaagtatttg gctcatctat aaaatttttc aaacaagata ttcattaaaa ggctctaaaa
     3061 ttcgatttaa gcgatttttc tttcttggct gtatattttc cttgataatc gtttcaagcg
     3121 gcttattcgg cgttttagag ggaaataagc gcgtttctag aagtatttta ttaggaaatg
     3181 tcactcaaaa gtatgaatca gcgcgtgata aaaagaaaaa agagcaagca ttagcacaaa
     3241 aaatggaaga gtttaccact tgttatgagg aaatgaacga tatttttgtg aatcaagaaa
     3301 aacgtttgac ggataaaaac atggaaaaac tcactcgtct ttatcaaaac ttgccagaaa
     3361 aacagcaaaa agaggttcaa gacaactatg agcgaaccaa aaaagacgtg caatatgtaa
     3421 aagatacaaa gatagaagaa gcctgtagtg atctatttgg agacacaaac ccttggtttg
     3481 ccagtgaaga agaaaaaaaa gaaaaacaac aatctgtcac atatgaacgc tatgaaatct
     3541 tacttcaaca agcaactaac atgcaaaatc cgacaaaaaa agaaaccgcc ttaaactatc
     3601 taaaaagtgt caaggaatgg ttagatcagc aacaacaaaa ttgaatttga tttaaaatca
     3661 tgttaaactg aatgtgcaaa tgatcaaaca ttagatacac gaaagcccca gagaggtcgg
     3721 attctctggg gctttttttc aggttgatgt cgcctttatt gattttactt cttgtttcgg
     3781 ttattcaacc aataactgaa taaagagaca caaactccta caagaagggg gcaaatgaac
     3841 agttcaaaca ttagatacac ctccttccag tcgcaagact gtcggatagg cgacaagtta
     3901 ttatatctta aaaaattaaa tttgtgtggt gtttttaagt atcacttgtt ttagggttgt
     3961 tagggcttgc cctgaccgcc tgtaaggcgc ttctcgacta gattaaggga aatttaatca
     4021 agaattgtct tagaacggct tagacaagct ttcagagtct cacacctcct ctttgaacaa
     4081 cggttttagc aaaggagccg gaggcgactg cgcagtataa tatgagagca tagcgatcac
     4141 acctttttag cgaccgtagg gagcaagttt tccagtttag aaaaattcgc gaaccttttt
     4201 ggaggaaaaa gtaggagttt tttaggcggg aaattcaatg tgctcttaag atatactgtt
     4261 agatatactg ttagatatac tgttaataat aactgttagg gattagcaac cttatgggag
     4321 agtaagtatt gacataggta gtgggggata aattgtcaca ctatggggga taaattgtca
     4381 cactatgggg gataaattgt cacactatgg gggataaatt gtcacactat gggggataaa
     4441 ttgtgtttta catcctctta tgtctatgat aacataattt attgaggtga tttttattga
     4501 gtaacgatat agttcgatat aataatggat taaattctgt ccctttaaga aaatttaccc
     4561 ctatagatat ggatttattt tggggaatat gttctaaaat gaaaagaaaa ggtacaaaag
     4621 aagaaatttt tacatttaaa caaataaaag aattaattga ttacaatata aatgcacgtg
     4681 gcgaccgttt tgctaatgat ttaataaaaa tgtcaaaaaa aatcattact ttgtctttta
     4741 tttatgaaga cgaaaaaaca tatgaacaat taaatctgtt tcaaaaattt aaaatcgata
     4801 aagtgaatga aacattatta ataaaggttt ctgaagaatt tgaatatata cttaattcaa
     4861 tcggaactaa ttttactcgt ttcgaattag aaaatatgac aaacttaaag agtagctacg
     4921 ttaaagagtt atatcgccaa cttatggctc ataaagacag aagatctaaa aaaggtcact
     4981 ggtttgtaaa aatagaagat tttagagctc tattagcaat accagaaagt tatcgcatga
     5041 gagatattga taaacaaatt tttgcccaag cgaaaaaaga atttttaaac gttaattcaa
     5101 ctcaccctgc tatttttaaa acttttgaaa tcaaaaaaat taaagcctac aaaggaaata
     5161 agataggatc tctgcaaatt tattttgaag aaaatatacc ttcaattacc ttagataatt
     5221 ggttagagaa ataataccta acaatttagg aggcaattat catgcaaccc aaagaaacct
     5281 ttacaataaa ggaattatct gaattatttg aaataagtag gcaagcaatc tctaaacatg
     5341 ttgataaatt agacccgtcc ttattagcta aaaatgagcg tggttataag gttgtattac
     5401 gttctggagt attgcaactt gctagcaact taggtaacca aaggttacta tataggttgc
     5461 atgagtatga acaagaaaac gataaaaacg tttatatatc aagcaaaaac gtgattgtaa
     5521 acgagttgct agataagttg cgtgaagaaa atgaattttt acgagaacaa ctttccctca
     5581 aagagcaaaa taattctaat atgcaaaaat tactcgacca gcaacaacag ctcaccctac
     5641 aggcaaatca acagatcgaa aaactgcaag aacagttaca attagcttat acggaagaaa
     5701 ctccagaaga tgaatcaact actattcttt ctgaagaaac agaatcgata gaaaaacaca
     5761 caaaaaagaa gaagcaaaaa tggtggcaac tttggaaata actggaggga acattcatga
     5821 caagaaaaaa tcttctattc ggttctattt tactaggaat tctaggcttc agtccgatgt
     5881 tattgtcagt aatcaataaa gggaataaac atggtgaatt gaaattgaat ccaaattatt
     5941 acaacagtcc taacgaagaa ttgtttgtaa aagagacgga taaataataa aaaagactgc
     6001 tcttccgagc agccagttaa aaaataatga gtgtttattc ttgaaaaagc acgcgcttat
     6061 gaagcgctac caaaaattta ccatatttta caaaaaataa caagtcttaa aaaaatgaca
     6121 gataaaaatg cacaaagaat tagatataga taaccgcgaa aaatcaagct ttagaaagtt
     6181 gcttttagct agaccaatta aaaatcacaa agtgagcgtc tctatccatt ggtagagggg
     6241 ctacttggca tagtgtacat tatgtcaact atgcaattaa ataactaact acttcatatt
     6301 agcttaataa aataatcaaa ataactaggg ggaaaaacat tgtataaaaa aatttttcat
     6361 tttttaaata agaatagtaa atataaaaat aatttaagat atctattact attaatccag
     6421 atagagcaaa ccaagtaaat attccatgtg taaagctaaa aaaattcatt tgaatcaatt
     6481 caaataaacc taatatagta acaaatatat taaaaataat agctgtcaat accagtaaat
     6541 tatttttttt attttttgaa ttatcttcat ttaaaataga atttatgtta gtactataaa
     6601 attcacaaat tttttttagt gtctctatat ctggtgttgt tttgttattt tcccactttg
     6661 aaatagtttg acgtgtaacg ttaaattttc ttgccacata ttcttgagta tacccttttt
     6721 tcactcttaa ttctcttagc ttagatccta aatcctccat tttttctcct atgtaaacga
     6781 ttatttacat aaaattttat cataaaaata ctaacgcgcc aaaaaagttc tttattttat
     6841 aaaacacact aaaataaaat aattctagta aaaaagatag gagaaataag ttatgaaaaa
     6901 atttttatgt ctttctgctt taatgggtgt cttattaact agtggaggaa tagctagtgc
     6961 cacagaagct ttaaatctag atgtacccga agaccatacc gccatttacg gaggaggaat
     7021 ggaagctaca gaagaaggat tttcttctag taaattgaga tatgctgctg gcggcgggga
     7081 tttcaactgt ggggttaatg gatttaaagt ttatgcaaat tactaccatg ctagagctaa
     7141 acatagtgca acagctaaaa atggacgagg cggtcaagta cgatcagttc agaaagctgg
     7201 agtaagagct tatgcaacat gcaatgccac acttactgga aatactggtt ggtggaatgt
     7261 ttattaaaaa agagagttta aactctcttt tttatttaca tttgaaagga aattcaaaat
     7321 atgttaaaaa aaataaacgc tgttttatca ctttttgtct gcattacaat actatttttt
     7381 tctactaatg ttataaatgg tataaagact tctgaagcgc tatataaaaa taccgctgaa
     7441 attcatatgc aatataaaga tgatatggac tttaacagtt atataaacgc tttgtatgaa
     7501 atatctacac aagaaaatat taatatttct caatatagtt ttactgctgt taaccaacta
     7561 actattcttg ctacaaaccc taacgctaac aaacactgga aattttctaa aaaaaatata
     7621 ttagcaagaa acgattctaa agtccattat actaataaca aaaattctaa gtaccaatta
     7681 aaacttccaa ataatttttt aaatataaat atttatcctt tttctcatgt ggaaaatatt
     7741 ggattaagtg aaatattata tgtacaagga aactcaaaca aattaattcc tgtactgaaa
     7801 aaatatggaa cacctactat ttccaatatc aatcaatcag atacttttca aattaatact
     7861 aatattgttc ttgttatctg ctatcttagt atatttattg taattactac aattatattt
     7921 gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt taggctataa ctcttttgat
     7981 actataaaag attctattaa agagttaaac ctcttatttt gttctttgat tataactttc
     8041 attttatcta tttttttgag catatattac agtaacgaaa attttttcct atcatttctt
     8101 ctatgtagta tttttcattt tataattagt tttttattta tcatagttac tctaagtatc
     8161 tatcatttat ctagtataaa aaattatcta aaaaattcac gaccacttaa gttaaatctt
     8221 tgtatattaa atatctgtct attcttaagc atgatccttc tgttaatagc ttctacaaaa
     8281 gtaataaata cccataatga agccgaaaat aattctctta aatattggga aagaactact
     8341 aatttataca aaacaaatat tactaatcaa ttaaatagaa acaacaccgt agaagaaaat
     8401 aactacttga aaaaagctag taaatttgtt tataaaatac agaagaatta taaaactttt
     8461 attatcgcgc catacaatta cgcaactatc caagagaata ataaagaatt ttttattgga
     8521 caaaaagttt atccaaattt tgaagactat gttagtcaac ctgctggtgc aggaatatgt
     8581 gttgatttaa actatctaaa atattacaat cctatttctt ttgaagaatc tactgactta
     8641 aatttaataa actctgatcc actaactact tacattctag tacctaaaaa atataaagaa
     8701 tatgcaaaaa tgattgaaaa aaattattta gaagacatac agtttagact tgaggactct
     8761 cctcctcaag ctccttataa aaatcctaat ttgaaaaatt taaaaataaa actaatattt
     8821 gttaataata accaaaagta ttttagtttt aataccttat atggaaaggc aaaagataac
     8881 tatacaataa ctgatcccat agttagagtt attaatccag aaataacaga atcattattt
     8941 tggggaaata tattgactag tgatggtgga ttattttttg atcagaaaca tacttcaaac
     9001 caaaactttt ttaatcaaat taatccgtat ataaaagaat ttaacctaga aaatatcata
     9061 aatttttctt attctgtttt tcaagaaact ggtgaattat tatctcaaaa aaaagtaaat
     9121 aaattaaatg catatctaca atatctttta atattaggat tatttataat aattaatttt
     9181 caaatgattt ttgttagttt tactataaat tcaaaaaaaa tagctttaaa aaaaatattt
     9241 ggatattcac caataagtca agttttagat ttatgtttac tcccttttac aatagagtta
     9301 tttgctacta tgtgtttt
//
