LOCUS       NZ_LR135195             5275 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4438 plasmid 5.
ACCESSION   NZ_LR135195
VERSION     NZ_LR135195.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884718
            Assembly: GCF_900638825.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,905
            CDSs (total)                      :: 2,815
            Genes (coding)                    :: 2,731
            CDSs (with protein)               :: 2,731
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 47 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 19 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135195.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4438"
                     /db_xref="taxon:1352"
                     /plasmid="5"
     gene            complement(<1..439)
                     /locus_tag="EL354_RS15210"
     CDS             complement(<1..439)
                     /locus_tag="EL354_RS15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126309547.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRVV
                     FALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQAL
                     KIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELK"
                     /name="hypothetical protein"
     gene            complement(467..922)
                     /locus_tag="EL354_RS15215"
     CDS             complement(467..922)
                     /locus_tag="EL354_RS15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322920.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGYI
                     KVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQVQ
                     KFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
                     /name="hypothetical protein"
     gene            complement(930..1916)
                     /locus_tag="EL354_RS15220"
     CDS             complement(930..1916)
                     /locus_tag="EL354_RS15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002322919.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLAQ
                     TQLEATRRIFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPNHE
                     IYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQPKE
                     KFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNKGKRI
                     GFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVTNDDFS
                     ISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1901..2257)
                     /locus_tag="EL354_RS15225"
     CDS             complement(1901..2257)
                     /locus_tag="EL354_RS15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016113394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002323247.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLCK
                     SVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQQL
                     SQIEKGLIDLWEQL"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            complement(2813..3178)
                     /locus_tag="EL354_RS15230"
     CDS             complement(2813..3178)
                     /locus_tag="EL354_RS15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322917.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFMR
                     NASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLDYK
                     TFYPELLEFFQKLNSLE"
                     /name="hypothetical protein"
     gene            complement(3187..3348)
                     /locus_tag="EL354_RS15400"
     CDS             complement(3187..3348)
                     /locus_tag="EL354_RS15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322916.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLTE
                     IIELDSKE"
                     /name="hypothetical protein"
     gene            complement(3338..3487)
                     /locus_tag="EL354_RS15405"
     CDS             complement(3338..3487)
                     /locus_tag="EL354_RS15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002322915.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGEQ
                     KNDG"
                     /name="hypothetical protein"
     gene            complement(3501..4250)
                     /locus_tag="EL354_RS15235"
     CDS             complement(3501..4250)
                     /locus_tag="EL354_RS15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002322914.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVLD
                     YCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVERP
                     DGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGKYAL
                     IMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELSDKMS
                     VSFFVTTLKRGRKVVGYEITINDTSKE"
                     /name="replication initiation protein"
     gene            4965..5063
                     /locus_tag="EL354_RS15240"
     CDS             4965..5063
                     /locus_tag="EL354_RS15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_110958386.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
                     /name="type I toxin-antitoxin system Fst family toxin"
ORIGIN
        1 ctttcagctc tttatcgggc aaagtagcga tcacgtcacg atttttttgt acccaatccg
       61 ccgtaataac ttcgcttgga tagtcttttg ggttaaaggt tttcagcttt tcttgactgg
      121 caattttcaa tgcctgctcc agttggtccg aaattttatc aatttttttc tgttttgctt
      181 cggctttcgc ttctagttgc tctattttgc tgattttagg gcgtacagac agcgcaacgt
      241 ccggacggta tgaagaaatg ccgaccgaca caaaaagcgc tagaaacgca aaacagaggg
      301 caaaaacgac acgtttcagc tttggttcct caaatttttc taagatcgag cgaaaaatgc
      361 agaataaact atacgaaccg accaaaataa tcacgacagg aagaatatgc caccccattt
      421 tttcaaagaa cagtttcatt tgcgttttaa gccccctctc gttcgattac aggagcttta
      481 ggactgaaac aataagaata gcagtgccac cgataaaaag ccccacagag gctaatacgg
      541 acattaaatt cgctattcga ttttgtttca gctcttcggc agtcgctcga tcatcgttgg
      601 ctaaaaattt ttggacttgt gggcgcactt gccggctcaa ttcgtcagat acagatttgc
      661 tgatattacc gtagctatca agcttttggt cgatttcagc ggttcttttt tgaacgtagg
      721 tggcgtagcg gttcatttca gctttcagct cgctcatttt gggaatatct tcgccacctc
      781 gcaccttgat atagccttgt tttacgctgt tatcaaggct tttcagcgtt tcaatgactt
      841 cttgattttg ttgcttgatt tccgctagtt cctgcttcac tttttgaagc tcctgcttac
      901 ttgcaaaatc atctaacggc atatcgtcac tacctttcca tgccaccacg atcatgatag
      961 aacgggcgag aacgttgttg acgctcgatt tctttttgtc gttgcagctc ctcttttttc
     1021 ttgcgttctt cttgctgacg aatatcatta gcaagatttt tcaaaccgct gatagaaaag
     1081 tcatcattgg tgactttagg cttggtttca agcggttttt cgacttcttt ttcttgtttt
     1141 tgttgagaaa attcaagtgt tcgttcgatc gcagggcgct caaaatcaga acctaatttt
     1201 cgtccgccga ttgtgaaatc tttgccctca tcatcgaccc atttgaagcc aatccttttg
     1261 cctttattgt actcataaac ctcgacagca taggcttctt gaagattttt tttgaaagaa
     1321 tcaaaatcgg tactcatatc ttgtgcgccc aggatcatat gcttcatttt ttctcgatta
     1381 ctcattttgc cctcggcgtg catttcaaaa tcaacttgtt tgaatttttc tttcggctgt
     1441 tcaatgccaa aactcaataa aaccttatcg tttaggtctt gtaggtggta gccgacctcg
     1501 gtatcagagt gaaatttttt accggtttca gtgtcgatac tggcgaaaac aatgtgatta
     1561 tgaatatagc cggattctga atcggtatga gtgcccatgt agatttcatg attgggatag
     1621 ctttttgcga tttcatcaac gagtttagca ccgatctcat gcgctttttg cacgtccgcc
     1681 ggattgtttt tgtcaaacga attatcaaac gattgaaccc aatgaaaggc ttgaataccg
     1741 cctgttttgg cgaaaatccg tctagtcgct tctagctgag tttgagccaa tttattctca
     1801 gctgacagtt tgtgcgtata aatgcggtca cgatcaagct ttccatgccg atttacatag
     1861 ccaatcagcg cagaagcccc acgtttaccg ccttttaaag ctaaagttgc tcccataagt
     1921 caatcaaccc cttttcgatt tgcgaaagtt gctggttcat caaggctaaa tctgaacttg
     1981 aaggcatttg ccccttattc atgttaataa agcgagccat ctgattgaga tttccgccat
     2041 atttgcgcaa ctctttggca atttgtatcg ccccctcccg atcaacgttc ggcgctcgat
     2101 acttcatgcc aagtgccacg ctcttgcaga gctgtggcac actcatacca gtttgatccg
     2161 caatggcggt caaatactgg tactcgtctt cgctcactcg aaatttaatt tgccgactat
     2221 cttttcgatt ttcggcattc aatttctgct cgctcatctc atcactcctc aaataaaaat
     2281 gctccctatt cggtcgcttt tttcttctcg tcattcttcg tggtcgatat tcaaagctac
     2341 aaaaacgctc cctattcagt cgcctttttt ccgcaatgaa atctccctcg gttatgccac
     2401 tacaaaatga aagttcccta ctcggtcact tccctttttc cgctctataa ccgaatgatt
     2461 tattaacctc atcatactcc ctattcggtc gcataacaag gttaataaat catataatac
     2521 gcaagcaccg catttggtac ccaaatacat cgcttgtcga ggtggcgttg ccccctacga
     2581 actcccccaa tcacgcaaca aaaagtagcc tgttcggcta tttttattac gtgatttcgc
     2641 ctcagaaaaa gtagtctgtt cgactatttt tctactggct catgcggtca aacttgcacg
     2701 caagttgacc ttattttttt gtctgcccgc tgttcgcagg caaacaaaaa agcaagccaa
     2761 aaatcggctt gctcgttctt caaaaattga tcgcatggac tagctcgttc cgctattcca
     2821 agctgttcaa tttttggaag aactctagca actcaggata aaatgttttg taatccaact
     2881 tgtttcccca gttgtcttcc atccattttt cgagttgctt ttgaaattgc accgcttcgg
     2941 ctttcagctc gctaggcaat ttttcggcag aagtggccac cggctttgca gctgctggcg
     3001 caggtgcaga tccggcggac gacttcaatg cctttgacgc attccgcatg aaagtattgt
     3061 aggcggaata gctcttgaac ccttgttttt caaccatatt tgtaccgatt ttgactgaaa
     3121 gatcatgccg agcttcagct aatttttttt ccaaatcttc gatatccatt tttgccatgc
     3181 gtaaactcac tccttagaat ctagttcgat aatttccgtt agaagaagat caaattggtt
     3241 gatcgtttct tctgaaagtc caccggtgta ttcaataata gcgtcactat gcctttctaa
     3301 cagcttttca agctgttcaa cgtcaatcgt aactgtctta gccgtcattt ttttgttctc
     3361 ctttttcgtc ataactatga atcaaaaagc cgtcacgttc gccttttgca tagttcatta
     3421 gttctttcat agcttgatct agcgaagaat ttttctttgc catcttttta cgcagtcttt
     3481 ttttcaacta aatcactcct ctattctttg ctggtgtcat tgatcgtgat ttcatatcca
     3541 acgacttttc gcccacgctt taaggttgtg acgaaaaatg aaactgacat cttatcagat
     3601 aattcttcaa tagcacgagt aattactttt tgaaaaaatt gacctgcagg aaggcgtttg
     3661 tctttaccaa gaaaccaaga ttcccattct tccaaatcac ctgtaatcgt tgtgtgtttt
     3721 tcttttcctc tacggtagga ttcccagagt tttaacatta tgagagcata tttcccattg
     3781 atccgagata attcacctaa acgaaagcta taaaactttt ctttgagatc aagaacgtat
     3841 ggagctgaat cagcagaaaa tttgaatctt actaacccgt cagcataaaa atcaatccaa
     3901 gaaaataatt gcgtcattct gagaccttga gtaccatcgg ggcgctcgac acgaagataa
     3961 agcgcagtat tttcattaag ctttttgaaa gcttcagcta ttcgacggta agaatctcca
     4021 gaggtgttca acccaaaatg tttgataatt tctaatgcgg ttacctcgta ttcgtcatct
     4081 agttttgaat ctttagttac aaagctaaaa cagtagtcta aaactcgttg ctcaaacgat
     4141 ttaagccctc caaatgcctt agctaagtcg tttccttgca ctactaaata atcttgacgg
     4201 cttagtaagt cttttaatgc aatatcagct tttttatttg gttgtcccaa actgaaaatc
     4261 ctccttttgt acttttttta aaataatacc atttattcag tttttttgca atatttgaat
     4321 tttcaacgtg gaaaatagta tttttgacgt ggaaaatagt atttttgacg tggaaaatag
     4381 tattttcaac gtggaaaata gtatttatag gcgctacaaa cgctggtata aagccattcc
     4441 ttttccccta aagtatttta aagtatttaa aagtaatcta taaagtattc tctttaagcg
     4501 cgcgggtgag aaaaaaattt aaattcaaaa aaaaaaaaat cccgcccaaa ccagttgtct
     4561 atttttttcc aacaggttcg cgcatgaaat aaactgcttc tcacatcatc gacaatttat
     4621 cccctttacc tcgttcaatc ttacagccac aaggcataaa ggagtgatcg ctacgctctc
     4681 acattttgct tacagcagtc gcctgcgctc cttcgttaag gtcaaaaccg ttgttcaagg
     4741 agcggttggg ataggcgtaa aagcttgttg tagcaagttt ttagactgat ttagattaaa
     4801 tcgttagtct tgttatacag cgcctttaca ggcggtcagg gcaagcccta acgacccaaa
     4861 agccccatct ggtagaccat cttttttttg cagggagtga atgctatatt aatcttggca
     4921 ctaaagccaa ctaacgctta cacaggttgg gaggtgtatt ccgtatgtac gaaaaatttc
     4981 taacgttggt tgtcgcaccg ctatttgtcg ggctagtcat tgagctagtt tctcattggt
     5041 tggacgagaa agacgacaac taaggcttag tgccttccaa ctcacacgcc tagtcgcagg
     5101 gcgaaaaaaa agccagacac cgtcgcatgg tgtctggctt ttgtgcattc cgcactcgaa
     5161 aaaattctaa cgttttcgca tggttattat agcatggacg ttggcaaatc tcaatttcac
     5221 tgactattca gctcaaaata ggattctaca tcgtcaaatc gctttgtgta gcggt
//
