LOCUS       NZ_LR135194            11208 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4438 plasmid 4.
ACCESSION   NZ_LR135194
VERSION     NZ_LR135194.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884718
            Assembly: GCF_900638825.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:54:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,905
            CDSs (total)                      :: 2,815
            Genes (coding)                    :: 2,731
            CDSs (with protein)               :: 2,731
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 42 of 84
            Pseudo Genes (incomplete)         :: 47 of 84
            Pseudo Genes (internal stop)      :: 21 of 84
            Pseudo Genes (multiple problems)  :: 19 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135194.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..11208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4438"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            706..1443
                     /locus_tag="EL354_RS15145"
     CDS             706..1443
                     /locus_tag="EL354_RS15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002305239.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAYKGNK
                     IGSLQIYFEENIPSITLDNWLEK"
                     /name="replication initiation protein"
     gene            1471..2010
                     /locus_tag="EL354_RS15150"
     CDS             1471..2010
                     /locus_tag="EL354_RS15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002289340.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEETESI
                     EKHTKKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            2026..2196
                     /locus_tag="EL354_RS15390"
     CDS             2026..2196
                     /locus_tag="EL354_RS15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002292472.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(2508..2969)
                     /locus_tag="EL354_RS15155"
     CDS             complement(2508..2969)
                     /locus_tag="EL354_RS15155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002305240.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPDI
                     ETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAIIFNIFVTILGLFELIQMNFFSFT
                     HGIFTWFALSGLIVIDILNYFYIYYSYLKNEKFFYTMFFPLVILIILLS"
                     /name="helix-turn-helix domain-containing protein"
     gene            3102..3392
                     /locus_tag="EL354_RS15160"
     CDS             3102..3392
                     /locus_tag="EL354_RS15160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126309545.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGMEA
                     TEEGFSSSKLRYAAGGGDFNCGVNGFNRIRQKSPILYRWVMNAVRYEGYRW"
                     /name="hypothetical protein"
     gene            3478..3882
                     /gene="tnpA"
                     /locus_tag="EL354_RS15165"
     CDS             3478..3882
                     /gene="tnpA"
                     /locus_tag="EL354_RS15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3899..5047
                     /gene="tnpB"
                     /locus_tag="EL354_RS15170"
     CDS             3899..5047
                     /gene="tnpB"
                     /locus_tag="EL354_RS15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            <5218..5367
                     /locus_tag="EL354_RS15625"
                     /pseudo=""
     CDS             <5218..5367
                     /locus_tag="EL354_RS15625"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /name="lactococcin 972 family bacteriocin"
     gene            5421..7547
                     /locus_tag="EL354_RS15180"
     CDS             5421..7547
                     /locus_tag="EL354_RS15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002323538.1"
                     /translation="MLKKINAVLSLFVCITILFFSTNVINGIKTSEALYKNTAEIHMQY
                     KDDMDFNSYINALYEISTQENINISQYSFTAVNQLTILATNPNANKHWKFSKKNILARN
                     DSKVHYTNNKNSKYQLKLPNNFLNINIYPFSHVENIGLSEILYVQGNSNKLIPVLKKYG
                     TPTISNINQSDTFQINTNIVLVICYLSIFIVITTIIFAFSKMKEITLKKMLGYNSFDTI
                     KDSIKELNLLFCSLIITFILSIFLSIYYSNENFFLSFLLCSIFHFIISFLFIIVTLSIY
                     HLSSIKNYLKNSRPLKLNLCILNICLFLSMILLLIASTKVINTHNEAENNSLKYWERTT
                     NLYKTNITNQLNRNNTVEENNYLKKASKFVYKIQKNYKTFIIAPYNYATIQENNKEFFI
                     GQKVYPNFEDYVSQPAGAGICVDLNYLKYYNPISFEESTDLNLINSDPLTTYILVPKKY
                     KEYAKMIEKNYLEDIQFRLEDSPPQAPYKNPNLKNLKIKLIFVNNNQKYFSFNTLYGKA
                     KDNYTITDPIVRVINPEITESLFWGNILTSDGGLFFDQKHTSNQNFFNQINPYIKEFNL
                     ENIINFSYSVFQETGELLSQKKVNKLNAYLQYLLILGLFIIINFQMIFVSFTINSKKIA
                     LKKIFGYSPISQVLDLCLLPFTIELFATMCFQLIFSKNTYLYFIIFMIFLINLITSIII
                     YKKNSFKYFKENIL"
                     /name="hypothetical protein"
     gene            7544..8164
                     /locus_tag="EL354_RS15185"
     CDS             7544..8164
                     /locus_tag="EL354_RS15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]; GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_002303561.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTLL
                     NNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAKKF
                     NSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEPTGN
                     LDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
                     /name="ABC transporter ATP-binding protein"
     gene            8278..8415
                     /locus_tag="EL354_RS15395"
     CDS             8278..8415
                     /locus_tag="EL354_RS15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002303562.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESYC
                     "
                     /name="hypothetical protein"
     gene            8857..9237
                     /gene="mobC"
                     /locus_tag="EL354_RS15190"
     CDS             8857..9237
                     /gene="mobC"
                     /locus_tag="EL354_RS15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_002317440.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASGDA
                     QRLSENLKIMQKELQKIWQQLR"
                     /name="mobC"
     gene            9219..10133
                     /locus_tag="EL354_RS15195"
     CDS             9219..10133
                     /locus_tag="EL354_RS15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002305249.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10093..10371
                     /locus_tag="EL354_RS15750"
     CDS             10093..10371
                     /locus_tag="EL354_RS15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10373..11062
                     /locus_tag="EL354_RS15205"
     CDS             10373..11062
                     /locus_tag="EL354_RS15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002298772.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQATNMQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          707..1443
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9220..10130
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.671"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aataactgaa taaagagaca caaactccta caagaagggg gcaaatgaac agttcaaaca
       61 ttagatacac ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatatctta
      121 aaaaattaaa tttgtgtggt gtttttaagt atcacttgtt ttagggttgt tagggcttgc
      181 cctgaccgcc tgtaaggcgc ttctcgacta gattaaggga aattaatcaa gaattgtctt
      241 agaacggctt agacaagctt tcagagtctc acacctcctc tttgaacaac ggttttagca
      301 aaggagccgg aggcgactgc gcagtataat atgagagcat agcgatcaca cctttttagc
      361 gaccgtaggg agcaagtttt ccagtttaga aaaattcgcg aacctttttg gaggaaaaag
      421 taggagtttt ttaggcggga aattcaatgt gctcttaaga tatactgtta gatatactgt
      481 tagatatact gttaataata actgttaggg attagcaacc ttatgggaga gtaagtattg
      541 acataggtag tgggggataa attgtcacac tatgggggat aaattgtcac actatggggg
      601 ataaattgtc acactatggg ggataaattg tcacactatg ggggataaat tgtgttttac
      661 atcctcttat gtctatgata acataattta ttgaggtgat ttttattgag taacgatata
      721 gttcgatata ataatggatt aaattctgtc cctttaagaa aatttacccc tatagatatg
      781 gatttatttt ggggaatatg ttctaaaatg aaaagaaaag gtacaaaaga agaaattttt
      841 acatttaaac aaataaaaga attaattgat tacaatataa atgcacgtgg cgaccgtttt
      901 gctaatgatt taataaaaat gtcaaaaaaa atcattactt tgtcttttat ttatgaagac
      961 gaaaaaacat atgaacaatt aaatctgttt caaaaattta aaatcgataa agtgaatgaa
     1021 acattattaa taaaggtttc tgaagaattt gaatatatac ttaattcaat cggaactaat
     1081 tttactcgtt tcgaattaga aaatatgaca aacttaaaga gtagctacgt taaagagtta
     1141 tatcgccaac ttatggctca taaagacaga agatctaaaa aaggtcactg gtttgtaaaa
     1201 atagaagatt ttagagctct attagcaata ccagaaagtt atcgcatgag agatattgat
     1261 aaacaaattt ttgcccaagc gaaaaaagaa tttttaaacg ttaattcaac tcaccctgct
     1321 atttttaaaa cttttgaaat caaaaaaatt aaagcctaca aaggaaataa gataggatct
     1381 ctgcaaattt attttgaaga aaatatacct tcaattacct tagataattg gttagagaaa
     1441 taatacctaa caatttagga ggcaattatc atgcaaccca aagaaacctt tacaataaag
     1501 gaattatctg aattatttga aataagtagg caagcaatct ctaaacatgt tgataaatta
     1561 gacccgtcct tattagctaa aaatgagcgt ggttataagg ttgtattacg ttctggagta
     1621 ttgcaacttg ctagcaactt aggtaaccaa aggttactat ataggttgca tgagtatgaa
     1681 caagaaaacg ataaaaacgt ttatatatca agcaaaaacg tgattgtaaa cgagttgcta
     1741 gataagttgc gtgaagaaaa tgaattttta cgagaacaac tttccctcaa agagcaaaat
     1801 aattctaata tgcaaaaatt actcgaccag caacaacagc tcaccctaca ggcaaatcaa
     1861 cagatcgaaa aactgcaaga acagttacaa ttagcttata cggaagaaac tccagaagat
     1921 gaatcaacta ctattctttc tgaagaaaca gaatcgatag aaaaacacac aaaaaagaag
     1981 aagcaaaaat ggtggcaact ttggaaataa ctggagggaa cattcatgac aagaaaaaat
     2041 cttctattcg gttctatttt actaggaatt ctaggcttca gtccgatgtt attgtcagta
     2101 atcaataaag ggaataaaca tggtgaattg aaattgaatc caaattatta caacagtcct
     2161 aacgaagaat tgtttgtaaa agagacggat aaataataaa aaagactgct cttccgagca
     2221 gccagttaaa aaataatgag tgtttattct tgaaaaagca cgcgcttatg aagcgctacc
     2281 aaaaatttac catattttac aaaaaataac aagtcttaaa aaaatgacag ataaaaatgc
     2341 acaaagaatt agatatagat aaccgcgaaa aatcaagctt tagaaagttg cttttagcta
     2401 gaccaattaa aaatcacaaa gtgagcgtct ctatccattg gtagaggggc tacttggcat
     2461 agtgtacatt atgtcaacta tgcaattaaa taactaacta cttcatatta gcttaataaa
     2521 ataatcaaaa taactagggg gaaaaacatt gtataaaaaa atttttcatt ttttaaataa
     2581 gaatagtaaa tataaaaata atttaagata tctattacta ttaatccaga tagagcaaac
     2641 caagtaaata ttccatgtgt aaagctaaaa aaattcattt gaatcaattc aaataaacct
     2701 aatatagtaa caaatatatt aaaaataata gctgtcaata ccagtaaatt atttttttta
     2761 ttttttgaat tatcttcatt taaaatagaa tttatgttag tactataaaa ttcacaaatt
     2821 ttttttagtg tctctatatc tggtgttgtt ttgttatttt cccactttga aatagtttga
     2881 cgtgtaacgt taaattttct tgccacatat tcttgagtat accctttttt cactcttaat
     2941 tctcttagct tagatcctaa atcctccatt ttttctccta tgtaaacgat tatttacata
     3001 aaattttatc ataaaaatac taacgcgcca aaaaagttct ttattttata aaacacacta
     3061 aaataaaata attctagtaa aaaagatagg agaaataagt tatgaaaaaa tttttatgtc
     3121 tttctgcttt aatgggtgtc ttattaacta gtggaggaat agctagtgcc acagaagctt
     3181 taaatctaga tgtacccgaa gaccataccg ccatttacgg aggaggaatg gaagctacag
     3241 aagaaggatt ttcttctagt aaattgagat atgctgctgg cggcggggat ttcaactgtg
     3301 gggttaatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     3361 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     3421 cctttttatt ttgtcccatg ttacaatcag tctatacaag aaatgagttg atataacatg
     3481 gaattagata caaataatca ttcagtattt cttctttatt accaccttgt tttagtaacg
     3541 aaatatcgta gacaagtgat tgatgatgaa atatctgatt atgccaaaac tacttttgaa
     3601 agaatttcag aatcgtatca cattacttta gtggagtgga atcatgataa agaccacgtt
     3661 catattatgt tcaaagctca acctaaaaca gaattgacaa aattcatcaa tgcctataaa
     3721 agtgccagct caagattgat aaaacgtgac tttccaagag tcaaacaatt tctttggaaa
     3781 gaaatgttct ggtctaaaag cttttgtctt ctgacaactg gtggcgcacc aattgatgtg
     3841 attaagaagt atatccaaaa tcaaggaaac aatcataaat agaaagtagg tgaactttat
     3901 ggaacaacta aaagcataca aattcagaat ctatccaaca gaggaacaag aaatcttctt
     3961 tgccaaatct tttggttgtg tccgtaaggt ctacaatcta atgcttgatg accgaaaaaa
     4021 ggcgtatgaa gaagttaaaa atgattcttc taaaaaaatg acttttccga caccagctaa
     4081 atacaagaaa gaatttccgt ttttgaaaga aattgatagc cttgctttag ccaatgccca
     4141 acttaattta gataaagcat acaagaattt ttttcgggat aaatccgttg gatttccacg
     4201 tttcaaaagt aagaaaaatc ctgttcagag ttatacaacg aataatcaaa atggtacagt
     4261 tgctttgatt gatagtaaat tcatcaaagt tcctaaattg aagtcattag taagaattaa
     4321 gcttcataga caaccaaaag ggattatcaa atctgctaca atatcacgtc attctagcgg
     4381 taaatactat atttctttgt tgtgtaaaga agaaattatc gaattaccta aaactaattc
     4441 cacaattggt attgacttag gtattatgga ctttgcgatt ctttctgatg gacaaaaaat
     4501 cgataataat aaattcacgt ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt
     4561 gtctaggcgt gctttattgg ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta
     4621 tcaaaaacaa aaacgcaaag tagctagatt gcatgaaaaa gtaatgaatc aacgtactga
     4681 ttttctgaat aagttgagta cagaaatgat caaaaatcac gatattatct gtattgaaga
     4741 cttaaacaca aaaggtatgt tgcgaaatca taaattagct aaaagtattt ctgatgtttc
     4801 ttggtcaaag tttgtgacta aattacaata caaggctgac tggtatggac gaaaaatcat
     4861 caaagtagat aaatggtttc cgtctagtca gatttgttca gagtgcggac ataaagatgg
     4921 caagaaatct cttgaaattc gagaatggac ttgtcctgtt tgtcatactc atcatgatcg
     4981 tgatatcaat gctagtatca atattctgac cgaaggtcta agactacatt ctatgggatt
     5041 ggcttagatc caatcatcaa gaaccgtagg aactacgggg atagcttggt aaataagaga
     5101 caccgctgtt agtaaagaaa tacgctatca agtatgctct attcccaaga agctcccact
     5161 tcaagcgtta agaaccgtca ggtttagcta agtggtgagt agttcacagt ttatgcaaat
     5221 tactaccatg ctagagctaa acatagtgca acagctaaaa atggacgagg cggtcaagta
     5281 cgatcagttc agaaagctgg agtaagagct tatgcaacat gcaatgccac acttactgga
     5341 aatactggtt ggtggaatgt ttattaaaaa agagagttta aactctcttt tttatttaca
     5401 tttgaaagga aattcaaaat atgttaaaaa aaataaacgc tgttttatca ctttttgtct
     5461 gcattacaat actatttttt tctactaatg ttataaatgg tataaagact tctgaagcgc
     5521 tatataaaaa taccgctgaa attcatatgc aatataaaga tgatatggac tttaacagtt
     5581 atataaacgc tttgtatgaa atatctacac aagaaaatat taatatttct caatatagtt
     5641 ttactgctgt taaccaacta actattcttg ctacaaaccc taacgctaac aaacactgga
     5701 aattttctaa aaaaaatata ttagcaagaa acgattctaa agtccattat actaataaca
     5761 aaaattctaa gtaccaatta aaacttccaa ataatttttt aaatataaat atttatcctt
     5821 tttctcatgt ggaaaatatt ggattaagtg aaatattata tgtacaagga aactcaaaca
     5881 aattaattcc tgtactgaaa aaatatggaa cacctactat ttccaatatc aatcaatcag
     5941 atacttttca aattaatact aatattgttc ttgttatctg ctatcttagt atatttattg
     6001 taattactac aattatattt gctttttcaa aaatgaaaga aatcactcta aaaaaaatgt
     6061 taggctataa ctcttttgat actataaaag attctattaa agagttaaac ctcttatttt
     6121 gttctttgat tataactttc attttatcta tttttttgag catatattac agtaacgaaa
     6181 attttttcct atcatttctt ctatgtagta tttttcattt tataattagt tttttattta
     6241 tcatagttac tctaagtatc tatcatttat ctagtataaa aaattatcta aaaaattcac
     6301 gaccacttaa gttaaatctt tgtatattaa atatctgtct attcttaagc atgatccttc
     6361 tgttaatagc ttctacaaaa gtaataaata cccataatga agccgaaaat aattctctta
     6421 aatattggga aagaactact aatttataca aaacaaatat tactaatcaa ttaaatagaa
     6481 acaacaccgt agaagaaaat aactacttga aaaaagctag taaatttgtt tataaaatac
     6541 agaagaatta taaaactttt attatcgcgc catacaatta cgcaactatc caagagaata
     6601 ataaagaatt ttttattgga caaaaagttt atccaaattt tgaagactat gttagtcaac
     6661 ctgctggtgc aggaatatgt gttgatttaa actatctaaa atattacaat cctatttctt
     6721 ttgaagaatc tactgactta aatttaataa actctgatcc actaactact tacattctag
     6781 tacctaaaaa atataaagaa tatgcaaaaa tgattgaaaa aaattattta gaagacatac
     6841 agtttagact tgaggactct cctcctcaag ctccttataa aaatcctaat ttgaaaaatt
     6901 taaaaataaa actaatattt gttaataata accaaaagta ttttagtttt aataccttat
     6961 atggaaaggc aaaagataac tatacaataa ctgatcccat agttagagtt attaatccag
     7021 aaataacaga atcattattt tggggaaata tattgactag tgatggtgga ttattttttg
     7081 atcagaaaca tacttcaaac caaaactttt ttaatcaaat taatccgtat ataaaagaat
     7141 ttaacctaga aaatatcata aatttttctt attctgtttt tcaagaaact ggtgaattat
     7201 tatctcaaaa aaaagtaaat aaattaaatg catatctaca atatctttta atattaggat
     7261 tatttataat aattaatttt caaatgattt ttgttagttt tactataaat tcaaaaaaaa
     7321 tagctttaaa aaaaatattt ggatattcac caataagtca agttttagat ttatgtttac
     7381 tcccttttac aatagagtta tttgctacta tgtgttttca attgatattt tcaaaaaaca
     7441 cttacttgta ttttataatt tttatgatct ttttaataaa tctaattact tctattatta
     7501 tttataaaaa aaatagtttt aaatatttta aggagaatat tttatgatat tatcagttga
     7561 gaatttcacc ctgtcaatta ataaaaaaaa gatcaaacaa aattggaact tttcaatatc
     7621 agaaaaagat tttattatag taacaggaaa aagtggtagt ggaaaaacta ctctgttaaa
     7681 taatttaagc ctaatagata aacaatatac cggtaactta ttctattttg aaaagaaagt
     7741 ttctaaacat gatatacaac gtctacgaag aaatcatatt agctatttat ttcaaaacta
     7801 tgctcttctt gaaaatcaaa cagtacaata caatttcgaa ttagcaaaaa agtttaacag
     7861 taatatttcc aatgatcata tttataattt attaacagat ttttctttgc caaaagatat
     7921 cttaaaagaa aaaatctttc tcttaagcgg tggagaacaa caaagaatag cattaattag
     7981 aaatatgtta aaaccatttg atatattatt tgcagatgaa cctacaggaa atttagataa
     8041 agaaaactca acttttataa tgaactttct gaaaaattta acagaaaaag aaaaaaaagc
     8101 cgttgtacta gtaacacatg acttaaatgt tctaaactat ggaactaata ttatagaatt
     8161 ataattttta cccgtgatgc tagctgattt aaaactagca ccacgggtat ttattttata
     8221 ttccatctct cctatcccaa aatttaaata atcacttaat aaaaaggagg cacccaagtg
     8281 attttattga aaaaagagta tgaaaaattt tatgatagac ttatttattc tttgcaattg
     8341 ccaaaaaaat taaaccgtca attacaaaag acttatgaaa ctaaatcaac tgaagaatgg
     8401 gagagttatt gctagatttt tggtagaagt aacaaaaagt ggacatatta tcataatgac
     8461 tcgccgaaag tctgataaaa aattggctaa aaatattgct gaatgtttaa taaaactatc
     8521 agttttagct aaatagttag tagtttacac aaaaaagcat ttatgttttt tgttgcaaag
     8581 caacaatttc atcttcggtg aaataagcga aactttacaa aaagtggcta gcatttgcta
     8641 gctttttttg tattgtggag cgtggctttt gagggggatt caagggggag cttgcgcccc
     8701 tttgacaagc ggttatttgc actaaatacg taggtatttg gctagcaaat atagagcttg
     8761 ccaaaccctt ttagtgagct ttactcattg taccttttta ggtacaatgg tattgcgaac
     8821 gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa tcaaaatttg
     8881 gctagcaatt cttctaaaaa atatacctat cgatccgagc caaaacaaat cagttttcga
     8941 gtgagcgaat ccgaatttgc aaagctgaag caatcggctg aagctttgca aatgagtgtg
     9001 ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa agtggcgcca
     9061 gacattgccc aggcaatcgc gcgcgattta gccaaagctg gagggaatat caatcaaatt
     9121 gctaagtggt gcaatacaca ccaacatgac gttgcttctg gtgacgctca acgtttatcc
     9181 gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacaatt aaggtaagcc
     9241 gaaccacttc ttgtagtcgt gcaattaatt acgcagaacc acgagcaacc attaaaacag
     9301 gaataaactg tgacgtcgat tatgccaaaa gtgaaatgaa acaaattcgc atgctgtatg
     9361 gaaaagacga ccgtgtacag gcacacttat tgattcaatc ttttcgacca ggagaaatta
     9421 ccgctgaaaa agctaatcaa ctaggggcag agtatgcaaa aaaaatagcc cccgatcacc
     9481 aaatagctat ttacacacat acagacaaag accatattca taatcacatt gtcattaact
     9541 ccgttaatct ggagactgga aaaaaatttc aagctcatgg tcaagcattt ctaaacaaat
     9601 gttatgacct aaatgacgag atttgtcttg cacataactt accaatcact gagcgaggaa
     9661 agaaaccaga aaaacgaacc atgtctgaaa taaaattaaa agaaaaaaat gaacctgttt
     9721 ggaaagacga aattcggatt gcgatcgatc aaacaatgaa aaacaaaaaa accagaacct
     9781 atgaccaatt ttgtaattcc ctcaaaacat tggggattca ttgcttcaat cgcgggaaaa
     9841 atttcactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaga ctaggaaagg
     9901 actacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgaacc tataaccaac
     9961 agcgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca gaagaagtac
    10021 gaagagaaca acgaactctt acgcctgaac aaccggaaat tagaccggct cgaagaaacc
    10081 atgaacaaca cgttgaaaga ggaacagacc accaacaaga attatcgctc taaaacgtta
    10141 aaagacatag aaaacgaaat aaagaaacaa cgtgcttttc tcgttcctta tacgcaagaa
    10201 aggatacaaa ctacgattga tttacaagtt aaaaaactcc atcaagagct attagaccag
    10261 cgaaataagg aacagaaaaa ggtgcttatt caacgatcat tggattttct tgccccctta
    10321 tgttgcttag tcctgacact gttcgcattt tggttagtat ggggaggcta aaatggcatt
    10381 ttatttaccc tatctactca tttttgttag tatttcagga agtatttggc tcatctataa
    10441 aatttttcaa acaagatatt cattaaaagg ctctaaaatt cgatttaagc gatttttctt
    10501 tcttggctgt atattttcct tgataatcgt ttcaagcggc ttattcggcg ttttagaggg
    10561 aaataagcgc gtttctagaa gtattttatt aggaaatgtc actcaaaagt atgaatcagc
    10621 gcgtgataaa aagaaaaaag agcaagcatt agcacaaaaa atggaagagt ttaccacttg
    10681 ttatgaggaa atgaacgata tttttgtgaa tcaagaaaaa cgtttgacgg ataaaaacat
    10741 ggaaaaactc actcgtcttt atcaaaactt gccagaaaaa cagcaaaaag aggttcaaga
    10801 caactatgag cgaaccaaaa aagacgtgca atatgtaaaa gatacaaaga tagaagaagc
    10861 ctgtagtgat ctatttggag acacaaaccc ttggtttgcc agtgaagaag aaaaaaaaga
    10921 aaaacaacaa tctgtcacat atgaacgcta tgaaatctta cttcaacaag caactaacat
    10981 gcaaaatccg acaaaaaaag aaaccgcctt aaactatcta aaaagtgtca aggaatggtt
    11041 agatcagcaa caacaaaatt gaatttgatt taaaatcatg ttaaactgaa tgtgcaaatg
    11101 atcaaacatt agatacacga aagccccaga gaggtcggat tctctggggc tttttttcag
    11161 gttgatgtcg cctttattga ttttacttct tgtttcggtt attcaacc
//
