LOCUS       NZ_LR135177            10188 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E4402 plasmid 4.
ACCESSION   NZ_LR135177
VERSION     NZ_LR135177.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884683
            Assembly: GCF_900638805.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 03:07:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,819
            CDSs (total)                      :: 2,729
            Genes (coding)                    :: 2,663
            CDSs (with protein)               :: 2,663
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 25 of 66
            Pseudo Genes (incomplete)         :: 37 of 66
            Pseudo Genes (internal stop)      :: 17 of 66
            Pseudo Genes (multiple problems)  :: 10 of 66
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR135177.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E4402"
                     /db_xref="taxon:1352"
                     /plasmid="4"
     gene            118..1611
                     /locus_tag="EL348_RS14715"
     CDS             118..1611
                     /locus_tag="EL348_RS14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            1746..2042
                     /locus_tag="EL348_RS14720"
     CDS             1746..2042
                     /locus_tag="EL348_RS14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(2066..3025)
                     /locus_tag="EL348_RS14725"
     CDS             complement(2066..3025)
                     /locus_tag="EL348_RS14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222573.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            3262..3942
                     /locus_tag="EL348_RS14730"
     CDS             3262..3942
                     /locus_tag="EL348_RS14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            4140..4745
                     /locus_tag="EL348_RS14735"
     CDS             4140..4745
                     /locus_tag="EL348_RS14735"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            4761..5333
                     /locus_tag="EL348_RS14740"
     CDS             4761..5333
                     /locus_tag="EL348_RS14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(5806..6378)
                     /locus_tag="EL348_RS14750"
     CDS             complement(5806..6378)
                     /locus_tag="EL348_RS14750"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_073120187.1"
                     /translation="MCQKNCCHSIISKENLLNQDFSTETICEKWAADITYIPTKKNGWC
                     YLSSIMDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREV
                     EQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGF
                     YNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="IS3 family transposase"
     gene            complement(6479..7641)
                     /locus_tag="EL348_RS14755"
     CDS             complement(join(6479..7365,7365..7641))
                     /locus_tag="EL348_RS14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            complement(8047..8301)
                     /locus_tag="EL348_RS14765"
     CDS             complement(8047..8301)
                     /locus_tag="EL348_RS14765"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000199136.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(8494..8769)
                     /gene="prgO"
                     /locus_tag="EL348_RS14770"
     CDS             complement(8494..8769)
                     /gene="prgO"
                     /locus_tag="EL348_RS14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(8741..9694)
                     /locus_tag="EL348_RS14775"
     CDS             complement(8741..9694)
                     /locus_tag="EL348_RS14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     Typing          119..1611
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttatttag tgtacttaaa ttaaatattc ttaaacactg aagagagaac acatactatg
       61 gctgagatta aagctaaact gtgttctctt tttgtttgag aaaggacgtt acaatcgatg
      121 ttgagcgaaa tttattcagc tattcttaaa aacggcttac gtacatacaa atatcgaaac
      181 agcaccatga aacctgcagg tcatattgat tccagtagga gtggtgctat ctttgcgtac
      241 cgtagcaagc aattaatgaa tgttgggcgt ggtatggtca ttacttcgga agaagctatt
      301 ttagagaacg aaaagaaact aacgcattgg acacctaata cctatcgctt tggtacttat
      361 gcggatgatt atcgaacagt ggttaaaggt cattctgaga aaaacttaag tcaaatcaac
      421 acatttgtag ttgatatcga ttcaaaagag aaccatcaag gcgaaattat tttagcctgt
      481 ttagatcaag tgggctacat gccaacgcta attttagaat cagatcatgg ttatcaagtt
      541 tattttgtac taaagacacc aggctatgtc acaaagaaaa gcaatttcaa ggtgatcgat
      601 gtagcaaaaa aaatttcaaa aacaattcgt ctacagttgg ctgaaaaatt gtccggagtg
      661 gatctaggat gtaaccactt cggaattgct cgattcccac aagaaaataa tattgttttt
      721 aaagaaatga actatcagta ttcttttgct gactggattc aatggtcgat gaagcttgaa
      781 tcagatcaaa aaacaaaaga agatcgagaa cgaaaactaa ttgtctttcc tgagaaaaaa
      841 gaaattcgtc aagtagatga accgtggttc gatatgttac ttcataaagc aaacatcgtc
      901 ggcggagaag gacgtcttgg tcgaaataat gtgattttca cgctttcttt agcttattac
      961 tcttcaggtt atgcattgga tacttgcgaa tacaatatgt tcacattcaa cgaacggtta
     1021 caggaaccgc tatctgaagc agaattagta aagattgtac gtagtgctta ttctggtaat
     1081 tatcaagcag caagcagaga attcattatt gaattgtgcc agcaatgggt ttcaccaacc
     1141 ttaaaaaata atgatctgtt cattcaacga cgtggctggt ggaaatttaa aaagccacga
     1201 gatcaaagaa aatatagtca taaaaatgaa tggaaagaag atctattggc ttatctaagc
     1261 gaaaaaagtc ttgctagtga gccttatttg aacctgtcaa aaaaagaatt ggcttcacaa
     1321 ctaacgatgc ccaagcgaac acttgataag atcttaaatg agttacgaga ggagaataaa
     1381 gtcttctaca aggtaacacg aggaagaaat ggcggaatga tcattgcttc agtcaaaacg
     1441 ttgttcgcga gggttattca actaagtaaa gaaaagagac tagattattc aacaaggatc
     1501 acagaggtat ttggactcaa cgagagctta gtagaagaga catttagaca attatcaacc
     1561 cccttgaacc ctcttaaaga agtgaatctt tttgaagtag atacaggttg aaaaataaaa
     1621 ggtgcaaatt gccttataca atatatctat gatacttctg taggtgttgc tccctttgcc
     1681 tagctacgtg taggtggtat acttataact gcgtatgcag tcttatgtat catggaggaa
     1741 gttgtatgtc gttaaaaaat tatgtctatt cacatcctgt taatgtgttt atcattggtc
     1801 gtttgggatt atctgtggaa gtgttctgtg agctttatgg ttttaagcag gggactttgt
     1861 cttcttgggt taccagggaa aaaacagttg cttctttgcc gattgagttt cttcatgggt
     1921 tgtccttagc ttctggggag actatggatc aggtatatga ttgtttgtgt gtgttagagc
     1981 aggagtatat tgagtataaa atagctaacg agttaagaaa aaggaagaag tatatccagt
     2041 agtagttttt atttactctt aattgtcaaa ttaagttaga caaatcatca atacttacgt
     2101 aactcaaaaa tacttccaaa ggtgttcgat aattcagtga ttttctagga atattatttc
     2161 gtttcgatgc gacagattga ataaaagatt catctactga attgaaatcc atagatttca
     2221 ataaaccatc tctacgtaac aatccgttag aattctcgtt taggcctctt tgagacggcg
     2281 ttcctggatc agcgaaataa atggcaatat cattggcatt actgatctgt ttccaatttg
     2341 aaaattcctt tccacaatca aaagtgatgg atttgaatag atttttcggt acagattcaa
     2401 accaatgatt taatttgttt tcaatatcaa tcgcttgtct accacacggt ttcaatgtga
     2461 tgataacttt tgataatcgc tcaactaagg taattacagc acttttatgt ttcaaaccta
     2521 cgatagtgtc accttcaagg tgaccaaact catttgagaa ttggctataa tctttttcac
     2581 gttcatgaat agagcgacgg aaagcttgtt ttccacgtct ttcttgatgt ccatttggtt
     2641 tacgcttgcc tttcatcggt aagtcagaag gattaaatag cccctttttg aacatacgat
     2701 aaatggtacg agcagaacaa cgaataggaa acgctgcacg accaacaatc acatcgggtg
     2761 tccatccttg aacaaccttt ctttgaatat attctgattg ttcctcaggt aaaacaagcg
     2821 ggcgtctacc acagtttgat ttgtttttct tatactgttg atagtactct aaggctgatt
     2881 ttccttgctt gaagaacagg tatactttat ggatcgtttg tcttgaacga ttcaagcaat
     2941 gcgcagtttt cgcaacagat tgatttattt tgaaataaga ctctatcatt acaagttcat
     3001 ccgttgtaag atgggtatag gtcatttgta ctcactcctt ataattcttt ggacggaact
     3061 attttgagtg tagcacaaat gactttttta gttgtctagc ttaattttac aatcggcgta
     3121 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
     3181 aggaaagggt tctgttgcaa agttttaaat ctactatcaa ataaggtaga ataatagaaa
     3241 aagatagcag gaggaatgac gatgaatcat tttaaaggaa agcaatttca gcaggatgtg
     3301 attattgtag ccgtgggcta ctatcttcgt tataacctta gctatcgtga agttcaagaa
     3361 atcttatatg atcgtggcat taacgtttct catacgacga tttatcgttg ggtgcaagaa
     3421 tatggcaaac tactctatca aatttggaaa aagaaaaata aaaaatcctt ttattcatgg
     3481 aaaatggatg aaacgtacat caaaattaaa ggaaaatggc attatttgta tcgagccatc
     3541 gatgcagatg gtttaacctt ggatatttgg ttacgtaaaa aacgggacac acaagcagcc
     3601 tatgcttttc ttaagcggtt agtgaagcag tttgatgaac cgaaggttgt agtcacagat
     3661 aaagccccct ctattacaag tgcctttaag aaactaaaag aatacggctt ttatcaaggg
     3721 acagaacatc gtaccattaa atacctgaat aatttgattg aacaagacca tcgtccagta
     3781 aagagacgca ataaattcta tcgaagttta cgcactgcct ctaccacgat taaaggcatg
     3841 gaagccattc gaggattata taagaaaacc cgaaaagaag gcactctctt cgggttttcg
     3901 gtctgtactg aaatcaaggt attattggga atcccagctt aaatcataga taccgtaagg
     3961 gattttattc tttatttaaa actttgcaac agaaccaact agactgtgca aaatagagac
     4021 acttgaaaaa tctatcgggg gtcagaaact taatagtttt tgacacataa acaaaatcgc
     4081 gatataatgg ggttgagagt gtttcataaa cttatgtcat aaagttaagg agatttcgca
     4141 tgattttaga gaataaattg ggattaacca atcaagttga gttggctaag gttgaagaaa
     4201 aactcagtaa acaaaaagcg aaagaattgt acgattgtgg aaaaataaat gaaatcgaag
     4261 ttggtacatt taaaggttta tctgaaattc atgaatttct attttcggat atttacgatt
     4321 ttgccggaaa aatccgatcg gtcaatattg caaaaggaaa ttttcgattt gcaccagtta
     4381 tgtatttaga acattcgtta cagcatattg atcagatgcc acaaacttct tttgaagaga
     4441 ttatcaagaa atatgtggag atgaatatcg ctcacccatt tcgagaagga aatggccgaa
     4501 gtactagaat ttggctggat ctcattttaa aggaagaatt gcaaaaagtt gtagattgga
     4561 acttaatcga taaagcagac tatctttctg caatggagcg aagtcccata aacgatttgg
     4621 aaatacgcta tctaatttcc aatgctctga ctgataaaat tgacgatcgc gagttatata
     4681 tgaaaggaat cgatgtcagc tatttctatg aagggtattc tgagtatacg atagatgatt
     4741 tgtaaaagta ggtggaccaa atgacgaaat atggatatgc acgtgtctca accgcttctc
     4801 aaagtacaaa agaacaaatt caacagttgc ttgagaacgg cgtagagaag aagaatattt
     4861 tttcagaaaa atttacgggt acaaaacggg atcggccaaa attcaatcag ctgttggctg
     4921 gtctgaagcc tggagatgaa atagttgtta cgaagcttga tcgatttgct cgtaacacga
     4981 gagaagcatt agagatcatt gaacctttac tcgatgataa cgtaacgatc aaggttttga
     5041 atctaggaac gattgaaaat acgtccatgg ggcgaatggt gacacggacg ttgttgagtg
     5101 tagcagaaat ggaacgtgat atgattgtgg aacgtaccca agaaggaaaa atgtttgcaa
     5161 gaaagaataa tcctaatttc agagaaggac gtccgaaagc aacaatcaca ccgaagaaga
     5221 ggcacgcata tgaactgctt acttcaggta aatcgtataa ggaagttgaa gctataaccg
     5281 gtttcagtcg cagtacgctt tttagaataa agaagaaaat tgaagaaagt taaaaaagaa
     5341 gtctcagcga accagacagg taaatactta atgcgttcta gatatgagtc tattagcatg
     5401 ttgtgaaata taatattttt ttatttactt gatttctgct caaaaaatgt gtatggtaac
     5461 tgtaaaaggt aaagtaatta aaggtatgct agccacgctt actatattag gataccatca
     5521 tatatttata atgaacgatt tgttcgtcta ccaaacaaca catatcgttc agcgtaagtc
     5581 acgtttagga acgtggcttt tttagtgtta ttatcgataa ataatctatt gactctattg
     5641 attttatctc catatcccta cggtgttgat tgtctttaga tctttcattt aagaaccaaa
     5701 taactatcat aaaaatcagc actgctgcta atataataaa cagcattcct attgtgatat
     5761 tactggattt gagtcaatat cttggacaca ttttagtaga gacggttaag ccattttttc
     5821 ttttgctaat tcttcaaatt cctgtggggt tagatagtga atcgagctat ggattcgatt
     5881 tcggttataa aatccttcaa tgtagctaaa tagcgctcga tttgcttctt caaaatctga
     5941 gtagctagtc gtgtagactt cttctttttt caatgaggca tggaaagatt cgattccagc
     6001 attatcataa ggtgttcctt ttctactata agaatgcctt attttgttgg ttttaagcca
     6061 ttgttccact tctcttgctg tgtattggct gcccaagtca gtgtgtagaa tcattccttc
     6121 tggaatgtga taatgtattt ttgctttatt caatgtttga atgacacaat ccacagtcat
     6181 tcgttttgaa aatgtataac taataattct tttcgtgtgt aaatccatga ttgaagataa
     6241 gtaacaccaa ccatttttct tagtaggaat gtacgtaatg tcagctgccc atttctcaca
     6301 gatcgtttca gtagaaaagt cctgatttaa gagattctct tttgaaatga tggaatggca
     6361 acagtttttt tgacaaattt tataaggtgc agaacttctt tccgtatgct attccgattg
     6421 tccttgacaa tgagcctcct cggatgtgac gatcttcggc aggagctaac agttaaagtt
     6481 agactgcctc gctagcgtta gcacatccga gctcattatc aaggattcgc tgcgccaatc
     6541 tctggttacg gtaaccaacc ggtgtctcgt agccaagtgt accgtgcaac cgaaggtggt
     6601 tccaccaatt gacatagtca aataactcca aatccaattg ttgtaaggtt tcaaatgtgt
     6661 attgatagac aaattctact ttcaacgact tataagttga ttcagctacg gcattatcaa
     6721 aaggacagcc tttatgactc aatgatcgat tgatgtcaaa agttgttaat aattcatcaa
     6781 tagcttggtt atcaaactct tttccacgat cagtatgaaa aatctcaacc tctgtcagag
     6841 gttgtttgat acggctaaat gcttttttta ctagaacggc atctttatgt tctccacaag
     6901 aatagccgag aatttctcga ttgaacagat ccaaaatgaa acagacataa ttccattttt
     6961 tcccgactcg tacataagtc aagtctgtta cgatcgcttc taatgggttg tctcttaaga
     7021 atttacgatt caatacgttt gtcgttttgg cttcattgca agtagaatga tgtactttaa
     7081 aataagcaac agtatagctc gattttaatc ctctattttt catgattcta ctaatttttc
     7141 gtcggctgat ctgaatgcct cgttttgata aggctttttt tatttttctt gagccgtagg
     7201 cctttcggct gcggataaat tcttcagcga ctacttcttc aagttctgat tcgtctttct
     7261 ttggttttga ttgataataa taggactgac gtgatagacc taatattctg cacatcgctg
     7321 atatagggta aagatgctta ttcgcatcga ttacttgtct cttcgtccga atatcagcgc
     7381 tgcttgcttt aaaatatcat tttccatttc taattgctgg tttcttttac gtagttctaa
     7441 caattctttt tgttcaggcg taagattatc tttttctttg aatgaaccac tcgttttaaa
     7501 ttgctttacc catttgtcaa atgctgaagc cgttagttca tattctcgaa tgatttctac
     7561 acgtggcttt ccagctaagt aaagattgac gatttgttgc ttgaattctt gtgaaaaagt
     7621 tcttcgtgtt ctcttagaca taaaaattcc tcctggtatg ttttcttcta gtctacacac
     7681 cttaattttt ctgtctagtt aattgtagcc tatccatgat cggtttatta gacctttgag
     7741 gtctatattt tttaactaca atcgacctta aatttaattg tttcattagc ttttggacat
     7801 gtttaagaga tactgaaatc ccttctttta ataaaacttg atggatcttt gtggcaccat
     7861 agcgttgttt attcgtaaaa aaaattgttg aaatcttctt tttcaactta ttatttctga
     7921 tttctgtagc tgtcaacggt ttattcttat aaaaataata gacacttcta gggatttcta
     7981 ataaccgaca caacaaagaa acagtgtaat ccttacacca tttttcaata aattgaacaa
     8041 gcgaatttag gttttccgag agaatatggc tgcggctttt tttaagattt caagttcctc
     8101 atttaaacga gcattttctt ttctcagttc agctacttct tttccagtca gtcctgtgga
     8161 ctgatcaggt aaatataaat tcttccattt gtaaatcgtt gcttcagata agccatattc
     8221 tttcgctaac cctcgaacag aacgtccagt ttgattcagt tcaacaatca tttgtttaaa
     8281 attttcttca tatcgggtca taaaaattca tcctctcgta gataagttta atttattctt
     8341 gtctctacga aaagctgtct aagatttgat actaacacca aatgttgtat acccccaaac
     8401 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
     8461 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
     8521 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
     8581 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
     8641 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
     8701 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
     8761 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
     8821 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
     8881 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
     8941 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
     9001 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
     9061 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
     9121 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
     9181 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
     9241 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
     9301 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     9361 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     9421 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     9481 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     9541 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     9601 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     9661 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     9721 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     9781 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     9841 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     9901 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     9961 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
    10021 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
    10081 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
    10141 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaac
//
