LOCUS       NZ_LR134112             8671 bp    DNA     linear   CON 13-FEB-2024
DEFINITION  Enterococcus faecium isolate E6043 plasmid 8.
ACCESSION   NZ_LR134112
VERSION     NZ_LR134112.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA4884790
            Assembly: GCF_900635415.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Arredondo-Alonso,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2018) UNIVERSITY MEDICAL CENTER UTRECHT,
            Heidelberglaan 100, 3584 CX Utrecht, Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/13/2024 02:29:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,131
            CDSs (total)                      :: 3,040
            Genes (coding)                    :: 2,901
            CDSs (with protein)               :: 2,901
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 65 of 139
            Pseudo Genes (incomplete)         :: 75 of 139
            Pseudo Genes (internal stop)      :: 34 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR134112.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /isolate="E6043"
                     /db_xref="taxon:1352"
                     /plasmid="8"
     gene            complement(547..888)
                     /locus_tag="ELZ65_RS16145"
     CDS             complement(547..888)
                     /locus_tag="ELZ65_RS16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_002347517.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYEN
                     KSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKLNR
                     FIERLQRKV"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(878..1159)
                     /locus_tag="ELZ65_RS16150"
     CDS             complement(878..1159)
                     /locus_tag="ELZ65_RS16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_002347516.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYKQ
                     VVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(1266..1850)
                     /locus_tag="ELZ65_RS16155"
     CDS             complement(1266..1850)
                     /locus_tag="ELZ65_RS16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025478671.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSNE
                     DIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYVAD
                     SYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDEIGM
                     CTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
                     /name="hypothetical protein"
     gene            complement(1867..2106)
                     /locus_tag="ELZ65_RS16160"
     CDS             complement(1867..2106)
                     /locus_tag="ELZ65_RS16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347514.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYMP
                     EERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
                     /name="hypothetical protein"
     gene            complement(2125..2373)
                     /locus_tag="ELZ65_RS16165"
     CDS             complement(2125..2373)
                     /locus_tag="ELZ65_RS16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347513.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLYD
                     DNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
                     /name="hypothetical protein"
     gene            complement(2388..2954)
                     /locus_tag="ELZ65_RS16170"
     CDS             complement(2388..2954)
                     /locus_tag="ELZ65_RS16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347512.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYYF
                     QGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTIEGEVLTGTRCVCCNHEFV
                     EDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELSKRS
                     DDEIEDWCVWLVGTDDCVAYYTTAE"
                     /name="hypothetical protein"
     gene            complement(2955..3500)
                     /locus_tag="ELZ65_RS16175"
     CDS             complement(2955..3500)
                     /locus_tag="ELZ65_RS16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347511.1"
                     /translation="MGERTQALITVKYGNSKTKVSVSIHYQWGYGRFMLMDVLNQGIYL
                     INKAYDLRDESREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIEDVM
                     KFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDFIKG
                     YRLLLENYGINLEEESVK"
                     /name="hypothetical protein"
     gene            complement(3526..4293)
                     /locus_tag="ELZ65_RS16180"
     CDS             complement(3526..4293)
                     /locus_tag="ELZ65_RS16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="WP_002320754.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRIG
                     RSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVENKT
                     ENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLNTKN
                     EVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSFTTNL
                     KNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
                     /name="JAB domain-containing protein"
     gene            complement(5266..5826)
                     /locus_tag="ELZ65_RS16185"
     CDS             complement(5266..5826)
                     /locus_tag="ELZ65_RS16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="WP_002320756.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQI
                     GLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADKLW
                     LIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKSEEE
                     QFFTEEELDKMLPEHLRKKSSKY"
                     /name="SLATT domain-containing protein"
     gene            complement(5846..6703)
                     /locus_tag="ELZ65_RS16190"
     CDS             complement(5846..6703)
                     /locus_tag="ELZ65_RS16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /protein_id="WP_002320757.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAIN
                     REFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVI
                     KNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNW
                     KATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNW
                     KFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLK
                     KIAL"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            complement(6936..8084)
                     /gene="tnpB"
                     /locus_tag="ELZ65_RS16195"
     CDS             complement(6936..8084)
                     /gene="tnpB"
                     /locus_tag="ELZ65_RS16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(8101..8505)
                     /gene="tnpA"
                     /locus_tag="ELZ65_RS16200"
     CDS             complement(8101..8505)
                     /gene="tnpA"
                     /locus_tag="ELZ65_RS16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
ORIGIN
        1 agatgtgtat aagagacagg tgcaatacga gcgaccgctc gctacgcgac cgttcgttcg
       61 tcttttcctc cgtgggactc acccaccgca agcggttggt tgagttgtgg ctcctattca
      121 aaattcacta atatgttatt tttgttttct attcaagtgg gtaacgttcg tttgcaagca
      181 aactctctct atgtaacttg ccagttacat cagtggtaac gctacgtcgc aagcgacttg
      241 ctctaatgaa actaccgttt cattctgcct gttcgttcgt agcaagctac ttcactcact
      301 gtatccgtct gaattattgt tatttattac tattcaatat ccgtcagtca ttttatttat
      361 ttacatacat taaatataaa atgtatctta taattcgtat attactaatt atcactattt
      421 aacaatactt aatattttct taatttttat aatcacgtat atatttacgt gtattttaat
      481 atacataaat acgtttatat aaacgtatat cttaatatac atataaacaa aaaaagaaaa
      541 gccttgttag acttttcttt gtaatctctc gataaaccta tttagctttt tcgtatcaat
      601 agctgatagc ataccaacct ttttaaatga tatgttctct cggcttttgg gaatagcctt
      661 taaagaccct acgtcaatcc atgattgctt ctttagacct actttttccc attctgcaat
      721 aggaaaatac actctcttta tttggtctga tttattctca tattgacttg tgattccaaa
      781 aaattctact ctatcttctt tatcactaac caccaaaata ggtcttcttt taccaccatt
      841 tttatctgta aatgcaacat agaccgttac tatctcatta ggcttcattg aaccactctt
      901 ctaacttttc atctgtatct agaacatcaa ctggcatatc catagttagc tgttgcaatc
      961 tgattccttg cttttcctct tcgcttagct ttatctctac tggaaactca cctctagcga
     1021 ccacttgctt ataaaggatt gtgattgctg ttgttggatt taaccctagc tcttccaata
     1081 tcatttctgc gttttctttt aaatccttgt ctattccgac ttgaattctg cttttttctt
     1141 ttggattttt taccttcatt tcgttcacct cttttgagta taatatactc attattatat
     1201 ccattaacta catttatgat tatattatac tcttaattag ctactttgca aacaatccaa
     1261 tcaaatcaag attctacttt actttttata cgttctttca tcttctggta tgcaacctct
     1321 actctttgga catctatatc caatccactt acaaccgtac acataccaat ctcatcgcta
     1381 tccgacactt tatcatattc ttcttcggtg atttcaatag gcatataccc atcattaaag
     1441 catatctcat catctgaata gttttcccat tcctcttcat catcttctga ataagtgaca
     1501 accctatctg ccccctgttc taccatatca tcgtaactat cagcaacata ctgcgctttt
     1561 aatcctttca gttgtaagtc atataaacca tacaacctct tactatcttg ttcactgaat
     1621 aaaattcttt gacgttctaa aattaattct ggttcatctt ccacctcttc atctggttct
     1681 gtaaaatacc attcgataaa ataatatcta atatcctcat tcgataaact ttcaaagtcc
     1741 atattctcct catcaaatcc atcaaaagcg ccactacttt gcataccatt caaccaatta
     1801 atggttctca atcttgcgta ttcacttaat accatcttat tctcattcat ggttcttcac
     1861 cccatattaa ttattttcaa attgacaaat caatttattt tgagttaaca aaactaatag
     1921 ctgttcacta tcaaaaagaa ccttacaaga ttctttttct attctctctt ctggcatgta
     1981 atagccaatc tgttttaaca tcttatctgg ataacctttt tgtttggctt ttctcaaaag
     2041 ctcttcatca ttcccaacat aataataaga acaccattgc atacctttac ttttgaattt
     2101 tgtcatgttg ctaccctctt tcgtttattg acccaaccat gaaaaaatat attttgctaa
     2161 atcttctttt tctatcccat ataaaacatt ctctaaatcc aatttatact cttcatcaac
     2221 aaggctttct gtattgtcat cataaaggac atagactgca taatttcttt cgtaaaaggc
     2281 attagcaaga cttctgctaa tacctaccat tcctttccat gtataaccaa aatcaatcaa
     2341 atcttcatga tctataaaat ccattccttc catgctgatt cacactccta ttctgctgtt
     2401 gtgtaatatg caacgcaatc atctgtccct actaaccaca cacaccaatc ttcaatttca
     2461 tcatcgcttc tcttactcaa ttcctctttg gtacaaaaaa ctacttcacc attttctgtt
     2521 tccatctcat aatcttgtag catgtattca aaccaacttc tttcggttgg aaattctatc
     2581 tcgtggcaac tctggcaaat aaaatcacca tattcattga ttcggtagca accttcatct
     2641 tctacaaatt catgattaca acaaacacat ctagtacctg ttaggacttc accctcaatc
     2701 gttgttctaa cggtatcttc tcttgctacg tgttcactgc tgacaagcca atcaatgatt
     2761 ttctttgcca attcttcatc agaatcggca aagcctttaa acgtgtttct ctttccttga
     2821 aagtagtatt tgcaacttgc ggtagtttct atggttccat aacttttttg gaataattca
     2881 atcatttcac catcaatcac tttttgactt ttcgcttcta aattagggaa ctggtctttt
     2941 cttttttcaa acacttattt cacgctctct tcttctaggt ttattccata attttctagc
     3001 aataatctgt atcccttaat aaaatctttc gtagcaaact cttttccgct ttcacaaaac
     3061 ttgtttagtc ctatctgttg accctcaaaa tcaaacaatc gcaatctggt tttatcatct
     3121 agactaaatc cattttcatc gaacaccatc tctaaatgag catagccatc attgttatct
     3181 gaaaacttca tcacatcttc aatcgtttct tgataaggtt catagcgccc accaaaccct
     3241 gtttcatcat aagcactcac tccagattca taggtaacaa aattgataac tcctgtactt
     3301 ctatcaaaga ttccttttgc taattcttct cttgattcat ctcgtaagtc atatgcttta
     3361 ttgattaaat atattccttg attcaaaacg tccatcaaca tgaatcgacc atatccccac
     3421 tgataatgaa ttgacacgct tactttcgtt ttgctgttac catatttgac tgtaattaac
     3481 gcttgtgttc tttcgcccat cttgttttcc tcttttctct aatttttata aagaaccatt
     3541 ttcttgaaat gagtaatagc ttgtgttttt acttgttacg atgatatggt ctaacaattc
     3601 aataccaatt gtttttcctg cgtttttcaa attcgtagta aaacttttgt cattttcact
     3661 tggaaattca tatccgctcg gatgattatg tgatactgcg atttttgctg aattgtttag
     3721 caaagctagt tgaaaaatct ctcttggtat cgctactgat tgattaattc cacctttgaa
     3781 aacttcgcta taagcaatga cctcattctt tgtattcaga cacatcacta gcaaagattc
     3841 actagcattg tttccaatta agttagtagc aaagtttgct agaccaaccg agcttgtgac
     3901 tctataaaaa cttctatctt cactgatata tttttgttcg attttcagga cattcttaat
     3961 caatccttca ccttcattct cggtcttgtt ttcaacttca atagattcaa gactgctatt
     4021 tcccatatca gaacccaaca aagcatttct gaaatccaat accttttcaa agttatcagt
     4081 cataaccatt tcataggtgc tattccattc attcaaatat ttctctttga tgagaatgaa
     4141 cgctctcggt ttacttctgc caattctgat attttgtgta tcgtgaaaaa tataattatc
     4201 aagtaaagta ccatcgtact tttcatagtt cccactgata tttgttaact tgtttaccat
     4261 ttcttgatta acttctaaaa ttctatctct catgttgaac gcttctttct gtttttgatt
     4321 tttggttttt caaaattctt ttttgtttgg attgttttag agggaggtgg ttttttcttc
     4381 atcgctattt ggttttgcat tttatgtttc ttcctttcgt tcatttattt atctttttcg
     4441 cctgtcttgc cttgtcggga gtgattagaa tgcgttctta tctgccggat taatacaagt
     4501 gtttaagtaa attattttcg taacgcttgc gtttagaaaa aaatttacgc cacttgtatt
     4561 aatccaaaga tgagataagc tctaaacact tctgacaagg caagacaggc gaaaaagata
     4621 atgggcgtaa ggaattataa atggcaaaac caaataaaga gcgatgaatt tcaatgcttt
     4681 caggcgtagc ttgaaagcct aaaagtcttg aagaatgaaa gactttttac cttcaaagca
     4741 gacctagtaa tcttaaatag gcgatggcta tttaagatta cggtgttgac ccactaaata
     4801 aacagaaatg gaaccgatca agcaaaatcg gactaggcac taggcgatgg ctaggacggt
     4861 ttgcggtggt tttaggaaac gttcatggtg agatgagcat atctagcaat gtcgggctaa
     4921 gctatgctta gaacggcttg cggtggcttt agaaaacttt tatggcgaga tgcactaatc
     4981 tagcgatgtc ggactaggca atggctagga cggcttgcgg tggctttaga aaacttttat
     5041 ggcgagatgc actaatctag cgatgtcgga ctaggcaatg gctaggacgg cttgcggtgg
     5101 ctttaggaaa cgttcatggt gagatgagca tatctagcaa tgtcgggcta agctatgctt
     5161 agaacggctt gcggtggctt taggagatgt ttctatcatc atatttcttc ttaaatatag
     5221 atgaatagaa aaattacaag agaattttaa gcataaaaaa agagttcaat atttagaact
     5281 cttttttcta agatgctctg gtaacatctt atccaattcc tcttctgtaa aaaattgctc
     5341 ttcttcagat tttaatgctt tctgagctga ttcatagctt ttactatttg ttttaggggc
     5401 atttttatat gcttcatgag tttcgttcat taattgatct ctttttttca caatatcttc
     5461 aacacttaaa cgatttaaat ctgttagtaa gcttacgtat tcttctctga tcaaccataa
     5521 tttatcggca acaatttgat gatttttagc ttcttcagca agatcaaaat tcttaaagta
     5581 taagtttaat ccaagagaaa ttgttgaaca taatccagca acaaaggtta actcactatt
     5641 atcagtaata atggtactaa gtacccctcc agtagtaagg gctgacaaac caatttgaag
     5701 acaccttaaa attttattgt cagaaataat attatcggct tgctttaaat gagtggtgta
     5761 agtatacgtt actcgtccat atgcttcacg taattgaact tccaagcgct ttttttcgtc
     5821 tcccatgttg ttcctccata aaactttata aagctatttt ttttaatact ttttctaaac
     5881 atatgatact actatcagaa ttaacaaatt gattgcttcc aggagagtat aaatttaaac
     5941 cgcccataac tttatgtgag ttatagtatt ctaataaagc tgtctcataa gatacagaac
     6001 taatgttttg acccccactg ttattttcaa cgaatttcca atttcccata gcctcatata
     6061 cgaaactgtc aattactatt cctgaaagat gataactcgt aaattctgta tctcgtacat
     6121 gtcgaaaatg tttacacgta gcatataata aattattact ttttgtattc tttaatttca
     6181 tggcttcttg ctcttttttg ggatttgtag ctttccagtt ccctcccatg tttgaatctg
     6241 gataaataaa cccttttgac tcaccccaat aatctttttc attaaaagct ggaactattt
     6301 caaatttgat tccatcatgg aaatttattt taacaacttg tccatcagct ctaatatcac
     6361 tatttggtag tatttcctga aatgcgtttt ttataacttg taataactta gacgggccat
     6421 ttgataaata atttttgaat ctgtcaaatt ctgccatcgg taattcaact attatatcta
     6481 tatcactagt actaattgcc gttcctctcc cataagaacc tacgtataaa ctgtgagcag
     6541 tatcactttc agaattccaa aactctctat tgatggctct agtaatcctt tgatatcttt
     6601 tagcaattaa ttggcgtttc gttaaaggta tagtataagt attagctctc ggaaccagta
     6661 accccattat aatctcatcc tttttaatta atttttcttc cataaattat gatatctaaa
     6721 tataatactt caagcaattt attttatata ttacttttat aaaaaatttg attaagtgaa
     6781 ctactcacca cttagctaaa cctgacggtt cttaacgctt gaagtgggag cttcttggga
     6841 atagagcata cttgatagcg tatttcttta ctaacagcgg tgtctcttat ttaccaagct
     6901 atccccgtag ttcctacggt tcttgatgat tggatctaag ccaatcccat agaatgtagt
     6961 cttagacctt cggtcagaat attgatacta gcattgatat cacgatcatg atgagtatga
     7021 caaacaggac aagtccattc tcgaatttca agagatttct tgccatcttt atgtccgcac
     7081 tctgaacaaa tctgactaga cggaaaccat ttatctactt tgatgatttt tcgtccatac
     7141 cagtcagcct tgtattgtaa tttagtcaca aactttgacc aagaaacatc agaaatactt
     7201 ttagctaatt tatgatttcg caacatacct tttgtgttta agtcttcaat acagataata
     7261 tcgtgatttt tgatcatttc tgtactcaac ttattcagaa aatcagtacg ttgattcatt
     7321 actttttcat gcaatctagc tactttgcgt ttttgttttt gataattttt agcttcaaat
     7381 agattggtac ctttattttt agccaataaa gcacgcctag acaatttgcg ttgttcacgc
     7441 tttagtttct tttccatttt ggacgtgaat ttattattat caattttttg tccatcagaa
     7501 agaatcgcaa agtccataat acctaagtca ataccaattg tggaattagt tttaggtaat
     7561 tcgataattt cttctttaca caacaaagaa atatagtatt taccgctaga atgacgtgat
     7621 attgtagcag atttgataat cccttttggt tgtctatgaa gcttaattct tactaatgac
     7681 ttcaatttag gaactttgat gaatttacta tcaatcaaag caactgtacc attttgatta
     7741 ttcgttgtat aactctgaac aggatttttc ttacttttga aacgtggaaa tccaacggat
     7801 ttatcccgaa aaaaattctt gtatgcttta tctaaattaa gttgggcatt ggctaaagca
     7861 aggctatcaa tttctttcaa aaacggaaat tctttcttgt atttagctgg tgtcggaaaa
     7921 gtcatttttt tagaagaatc atttttaact tcttcatacg ccttttttcg gtcatcaagc
     7981 attagattgt agaccttacg gacacaacca aaagatttgg caaagaagat ttcttgttcc
     8041 tctgttggat agattctgaa tttgtatgct tttagttgtt ccataaagtt cacctacttt
     8101 ctatttatga ttgtttcctt gattttggat atacttctta atcacatcaa ttggtgcgcc
     8161 accagttgtc agaagacaaa aacttttaga ccagaacatt tctttccaaa gaaattgttt
     8221 gactcttgga aagtcacgtt ttatcaatct tgagctggca cttttatagg cattgatgaa
     8281 ttttgtcaat tctgttttag gttgagcttt gaacataata tgaacgtggt ctttatcatg
     8341 attccactcc actaaagtaa tgtgatacga ttctgaaatt ctttcaaaag tagttttggc
     8401 ataatcagat atttcatcat caatcacttg tctacgatat ttcgttacta aaacaaggtg
     8461 gtaataaaga agaaatactg aatgattatt tgtatctaat tccatgttat atcaactcat
     8521 ttcttgtata gactgattgt aacatgggac aaaataaaaa ggcaatacgc cttagttgtc
     8581 ggtcttcgag ttaccaacgg taaccctcat accgaacggc attcatcacc cacctataga
     8641 ggatgggcga cttctgccta attctgttaa a
//
