LOCUS       NZ_LR130566             4082 bp    DNA     linear   CON 18-JUN-2024
DEFINITION  Escherichia coli strain MS14387 isolate MS14387 plasmid 3.
ACCESSION   NZ_LR130566
VERSION     NZ_LR130566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5128445
            Assembly: GCF_900622685.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Seemann,T. and Baines L,S.
  CONSRTM   Bioplatforms Australia Antibiotic Resistant Sepsis Pathogens
            Initiative
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2018) Bioplatforms Australia, Level 4, Bldg F7B,
            Research Park Drive, Macquarie University, NSW, 2109, Australia,
            Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900622685.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 04:38:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,106
            CDSs (total)                      :: 4,990
            Genes (coding)                    :: 4,806
            CDSs (with protein)               :: 4,806
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 70 of 184
            Pseudo Genes (incomplete)         :: 124 of 184
            Pseudo Genes (internal stop)      :: 30 of 184
            Pseudo Genes (multiple problems)  :: 37 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR130566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS14387"
                     /isolate="MS14387"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="3"
                     /geo_loc_name="Australia"
                     /collection_date="2009-09-03"
     gene            <1..702
                     /locus_tag="FVE81_RS25770"
     CDS             <1..702
                     /locus_tag="FVE81_RS25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281397.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(958..3117)
                     /gene="mobQ"
                     /locus_tag="FVE81_RS25775"
     CDS             complement(958..3117)
                     /gene="mobQ"
                     /locus_tag="FVE81_RS25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029343.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3296..3550
                     /gene="mobC"
                     /locus_tag="FVE81_RS25780"
     CDS             3296..3550
                     /gene="mobC"
                     /locus_tag="FVE81_RS25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2760..3118)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3119..3295
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..702
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(962..3204)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcggatc taattgccta taaatccaat gcgctggttg aagccagtta taaattgacc
       61 ctacaggaac agcgattttt gttactttgc atcggtcgat tgaagtctgg ggctgatgca
      121 gaatctccaa aattgcaaaa gacaatgacc attacagctg cggaatattt cgattcattc
      181 cctgacatgg ggcggaaaaa tgccgaggtt cagttacagg aagcaattga tcgtttgtgg
      241 gacaggtcaa tcattctcaa ggatgacgag aaacgtgagg agttccgctg gatccagtat
      301 cgggcacagt acgctaaagg cgaggccaga gcacaaataa cattttctga cgcagtaatg
      361 ccttatttga cacaactaca agggcaattt actagagttg taatcaagaa tatatctaga
      421 ttatcaagaa gttacagtat aaggatatac gaactcctcc agcagttccg cagtactggc
      481 gaacgtatta tcgctctcga tgattttcgc tcaatgcttg atatagagca taaatatcaa
      541 acatataaat cacttaacca gcaattatta agaccctgca ttgacgaact gaacaaaaaa
      601 agcgacctgg ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
      661 tttcgcttca aggaagacaa acaaatcaag atgacgatct aaaatcgatt ttaaggcacc
      721 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
      781 aaattgcgtc aaaagcgctc taatgcagcg aaaaaagaac ccccattttt tgggggcgga
      841 aaaacaaaac tatgcttgca ttatgtgtgt gcttcttgca ttcatccctg tcgatgaaca
      901 tcgaagaaca tcgacgaaca taacacgatg ttaaacaaaa caaaatattg ttcacattca
      961 ctgtgaccaa ccgcgatcat gtctcctctc ggtggcgtgt gttcgtgcat cttcctcgcg
     1021 ccgccattcg tcccaggtac gattaaaacc cagcgagcgg aacagatgat ccggctgttt
     1081 ttctggtgtg ttgacggcag caaaaagcgg tgcctcacgg gttttcagat gaaactccat
     1141 aagttccggc gcaggtgata tgtggctgtc tgcatcgtga tttacggcaa caccataccc
     1201 tgcataccgt aaccccttat cggtcagtgc actgatgacc agctggctgt tatcggctga
     1261 gggaacggca aacgcgggat acagcgtacc attaagccgg acaacaccgc ccagagagtc
     1321 atccacgccc atgaccagca aaagctgaag ctggcgggtt tcccggtcga cagaactcat
     1381 taccggcatc agcgtcggta gcagacgtcc ctgcgtaaaa tccaccttcc ttgctgccag
     1441 tgatggcatg gctggctggc ggtcttctcc aagacagaaa gactccggcc atgtcttcgg
     1501 ctggcggaga ttgcgggtat attgcggcca tgtctggcaa acctgcgtca gaagttgctc
     1561 aaaggtcgca gaggaataaa cacgttgcag tggtggatgt tgaccgattt cgcttcggtc
     1621 acagatataa ccactcctgt cacgcattaa atcaacacgc tcaaagattc tggtgcggta
     1681 tttctccagc tcgttccccc agagcgtgta ttctccgttc cggttgtgaa gcagaaggta
     1741 acaggtcatt tccccgttac caggcatggg ggcctcgcca taccccgtaa cgactgcgct
     1801 cagtacccct gaatccataa cggcattgtc atttttcctg agctgttccg ctcgtcgctc
     1861 cgcgcgacta cgttctgtat cctcctgctc cttccggcgc aattcctctt cacgtttaag
     1921 caactggact ctgagagctg caaggccgga tgccccccac tgctgccact gtttttcaaa
     1981 tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc cggtaatgct gtcgcaattt
     2041 ttccatttgc tggctcagcg actggatttt tccctgttcg ctacggtatt ccggtgccgc
     2101 ccattgcatc aggggaatac gggaaaaacc ggacgaaaac ggcgatgatt ttttcaggct
     2161 ggcagcatgg ctcatcgctt cctttcgttc ggtgttcagc tcttcccagc gtctgcgcgt
     2221 cgcctgcata tctgccatca tcgacatggc attactggtg agtttccatg ccgcattact
     2281 gtccttcggc aacgcttcga tgatgtcacg tagtgtgtac gaagttgcgt tgtcaggaag
     2341 cacgatattc agaaggtcag ccacacggcg ggcaggctct gtcagcccgc tgtttctgag
     2401 ttcggtacgg gaaatctgta tttccaggtt aagtctgtta atcagacgat tctgttcgcc
     2461 gcgttctgtt tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc
     2521 aggcgttttc tcaagcccct gtcgctcgta tgaacgatgg tctatctctt cctgataacc
     2581 ggcgttcgcc agtgccctgt tcgcatggtc agcccatgat gcacgccacg tctcggcatg
     2641 tgtccggtcg ttccagtccc tgacctttcc accgaatcca gcaggtccga cagctctcgt
     2701 ggtcagcatg atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc
     2761 aatcatgccc tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc
     2821 atcctgcggt agttcgcggg ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt
     2881 ccggcgttcg gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg
     2941 ggctgtcgcg ggagtcagaa tcacagcttc agccacaccg cttttgcggg tgtagtcgtg
     3001 agtcagtccg gtacgctcat caagcatcag tgaaccggaa cgataggcag cagaagcaac
     3061 ggcgctgtag ccgctgtttc tggagatgat tttcatgctg agatgataaa tcgccatttc
     3121 tgccgtaatg cctttttgtg gttttctttc agccccgcag ggcgcacaca atcgaaacgg
     3181 agtggagatt gtataagtgc gcacttcgtg attttccgtg cattatgcca gctcagtttt
     3241 taaactacca gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt acatcatgag
     3301 cagaacactc gaacagaaga ttgctgatgc tgaggccaga ttacagcgcc tgaaggcgaa
     3361 gagcaggagt ctggacacag cgcagaaggt cattgtgggt gcagcattgc tggcaaaggt
     3421 cagaaagccg gaggaggtgc agttgcgtgc ctggttactc cagttcctga aggcagaggt
     3481 gacacgacag gctgatgtga cgcgcatact gccgctgatt aacgagctgg aagcgctgcc
     3541 aggacagtga ttgtatcgtc cgctcgccgc agtcccagcg accgagcgtt agcgagcgag
     3601 tgggcgagga agcggaagtg aatccgtcgc ctgattgagc acatacgcac ggtttttcgg
     3661 gaggtgatat gcgctgaacc cgcatgaata ctggatttcc acagggttgg agggggtgta
     3721 ttcggaactt gcaattctgc ttttttgcaa cgcattgttt ttattggttt tttgttttca
     3781 gcactccaag ttatccacag gctcgtcgtt aagtttttag atcttgtttt taagatcttg
     3841 tttttaatac ttgttttagc gccaaaattc cttatagaaa acaaaatgtt acaatgctat
     3901 aggtaatgtg atttaaggta aaggtaatat gatttaaggt gtttaggtgg gggaatttaa
     3961 ggtaaaggca atatgattta aggtaatatt cacagaaaaa gggatattaa aataaaaagg
     4021 cattgccttt cgttgagggt atgtttatct tttattcatt accttttggg gagggatgct
     4081 ct
//
