LOCUS       NZ_LR130560             6850 bp    DNA     linear   CON 18-JUN-2024
DEFINITION  Escherichia coli strain MS14385 isolate MS14385 plasmid 6.
ACCESSION   NZ_LR130560
VERSION     NZ_LR130560.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5128442
            Assembly: GCF_900622655.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Seemann,T. and Baines L,S.
  CONSRTM   Bioplatforms Australia Antibiotic Resistant Sepsis Pathogens
            Initiative
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2018) Bioplatforms Australia, Level 4, Bldg F7B,
            Research Park Drive, Macquarie University, NSW, 2109, Australia,
            Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_900622655.1-RS_2024_06_18
            Annotation Date                   :: 06/18/2024 04:49:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,415
            CDSs (total)                      :: 5,294
            Genes (coding)                    :: 5,080
            CDSs (with protein)               :: 5,080
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 73 of 214
            Pseudo Genes (incomplete)         :: 149 of 214
            Pseudo Genes (internal stop)      :: 44 of 214
            Pseudo Genes (multiple problems)  :: 45 of 214
            Pseudo Genes (short protein)      :: 1 of 214
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR130560.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MS14385"
                     /isolate="MS14385"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="6"
                     /geo_loc_name="Australia"
     gene            <1..775
                     /locus_tag="EW040_RS27340"
                     /pseudo=""
     CDS             <1..775
                     /locus_tag="EW040_RS27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088970.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(844..2364)
                     /locus_tag="EW040_RS27345"
     CDS             complement(844..2364)
                     /locus_tag="EW040_RS27345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="WP_025745078.1"
                     /translation="MFLISIIQMFSVMIIMLNIKIKDAFRAIILLLLLLLSMVISIEIS
                     SIISIGEYIPTLALANISETNSVGWKIKLLPFLVFLSFMIASLILKRLIKEKKIRNIMV
                     VVFLLSLLIPISPINAFFSSSYEVYSEAKEYNMSPHEISDIKSKYTKEIVTNDSYSHEI
                     INGNGKNILVIFIEGMSSLVISKELTPNLYEFRKKSISFNNYYNHTAATFRGLRGQLTS
                     SYQMTGGYYKDNSGLGQASKVEIEHKLNTYNYITKLPIILEENGYHTYFQASNSIDAPL
                     SLMLSTLKFNHIYGREDYNKKSGELTDKESFDLLFKKLNEAKQPFFYGVYTVGTHLGLD
                     SPDVKFYDGRNEYLNHFHQLDHWFGDFISHFNKTSLSKDTIVIITSDHASYPSEDYKKT
                     FKTDNTFFVDQIPLIIWSNGVEFKDINAHGLNSLSLTPTILDILGIKESHNMFIGESLF
                     EKEKNKNELNCTSAIGDLYVKTCNGKMEIEKRKEKIELIKQLQLFGDR"
                     /name="LTA synthase family protein"
     gene            2657..3348
                     /locus_tag="EW040_RS27350"
     CDS             join(2657..2906,2906..3348)
                     /locus_tag="EW040_RS27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225905542.1"
                     /translation="MASISISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARFIGVGLNTIFRHFKKLRPQSVTSRIQPGRDVIV
                     CAEMDEQWSYVGAKSRQRWLFYAYDRVRRTVVAHVFGERTMFTLERLLSLLSAFDVVVL
                     MTDGWPLYESRLKEKLHVISKRYTQRIERHNLRQHLARPGRKSLSFSKSVELHDKVIGH
                     YLNIKHYQ"
                     /name="IS1 family transposase"
     gene            3593..3742
                     /locus_tag="EW040_RS27640"
     CDS             3593..3742
                     /locus_tag="EW040_RS27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162842177.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            4127..4495
                     /locus_tag="EW040_RS27355"
     CDS             4127..4495
                     /locus_tag="EW040_RS27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001273383.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLAGVDSVSVSETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(4892..5209)
                     /locus_tag="EW040_RS27365"
     CDS             complement(4892..5209)
                     /locus_tag="EW040_RS27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000718045.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAANVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="hypothetical protein"
     gene            6203..>6283
                     /locus_tag="EW040_RS28365"
                     /pseudo=""
     CDS             6203..>6283
                     /locus_tag="EW040_RS28365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="plasmid mobilization relaxosome protein MobC"
     gene            6511..6747
                     /locus_tag="EW040_RS27390"
     CDS             6511..6747
                     /locus_tag="EW040_RS27390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000138111.1"
                     /translation="MTEMERQLLSALESLQTSYEQQQQAWQDSYSSLQRMFETTSQALE
                     RNGRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          6081..6166
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.552"
                     /coverage="83.0"
                     /evalue="2.14e-32"
     Typing          complement(5607..6114)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.725"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcgcatcg tagggaaaga acgcgctatt ctggcgaaat acatccagtt caatcagcct
       61 cacgccatgt tctggctggg atttgatgtt gaccgcatcg gcgcagccat tgactggagc
      121 gaccgcaacg ctcccgcacc tacgctgacc atcaccaacc ccgaaaacgg acacgcgcac
      181 ctgctttatg cgctggaaac ctctatacgc acagcacctg acggaaaaat gaaaccgtta
      241 cgctacgcag ctgccgtaga gaatgcgcta cgtagaaaat tgggagctga cacgggatat
      301 tcggggctaa tttgcaagaa tccgaaccac agtcactgga aaattgccgc cgattatggt
      361 ttaggccgta actgcactct gttcgacaaa acccgcaagt gggcatatcg tgccatccgt
      421 caggggtggc ctgagtatga gcagtggtta caagcctgct atgaacgagc ctgtgcctac
      481 aatttacaat tcgtatcacc attaagtgaa aatgaagttc ttggaatatc aaaaagcatc
      541 tcaaaatgga cagcgaataa tttttcagag gatcgcttta ttgagtatgt aaaaaatacg
      601 caccgttcag aaattcaaag aaagagaggg ggtaaaggag gaaagctaag caattcagga
      661 ggacggccta aaggtagttt taatattaat tcctataact ttaaaaaaag aatgggagtc
      721 tttaggtgtt agtcgagcca cattctacag gcggctcaag aatgaaaaaa agtaagtttt
      781 attatcattt taaaataacc ccacgtggac taataatcaa catggggtaa aaccgccttt
      841 taattatcta tcaccaaaaa gttgcaattg ttttattaat tctatttttt cttttctttt
      901 ctcgatttcc attttcccat tacacgtttt cacatacagg tcacctattg cagatgtaca
      961 gttgagttca tttttatttt tttctttttc aaatagagac tcacctatga acatgttgtg
     1021 tgactccttg atcccaagaa tatctagtat tgttggcgtt aaagatagtg aatttaaacc
     1081 gtgtgcatta atgtctttga actcaacacc attactccat attattaatg gaatctgatc
     1141 aacaaaaaaa gtattatcag ttttgaatgt ttttttgtaa tcttctgatg ggtaacttgc
     1201 atggtcactt gtgattatga caattgtatc tttcgataag cttgtcttat taaaatgaga
     1261 tataaaatca ccaaaccaat ggtctaattg atgaaagtga tttaaatatt catttctccc
     1321 atcataaaac tttacatcag ggctatctaa gccaaggtgt gtacctacag tataaacacc
     1381 atagaagaaa ggttgtttag cctcatttaa ttttttaaat aataaatcga aagactcttt
     1441 gtctgtgagt tcacctgact ttttgttata gtcctccctt ccgtatatat gattaaactt
     1501 taaagttgat agcattaaac tcaatggggc atctattgaa ttagatgctt gaaaatatgt
     1561 atggtaacca ttttcctcaa gtatgattgg tagttttgtt atgtaattgt aagtatttag
     1621 tttatgttcg atctctactt tagatgcttg gccaaggcca gaattatctt tatagtaacc
     1681 accggtcatt tgatatgatg aggttaactg tcctcttaaa cctctaaatg tagctgcggt
     1741 atggttgtaa tagttgttaa atgatattga tttttttcta aattcatata aattcggggt
     1801 tagctcctta gatattacca atgaagacat accttcaata aatattacta aaatattttt
     1861 tccgtttcca tttattattt catgtgagta actgtcgttt gttactattt cttttgtgta
     1921 ttttgacttt atatcactta tttcatgtgg tgacatattg tattcttttg cttccgaata
     1981 aacttcatat gatgaagaaa aaaaagcatt tattggagat attggtatta acaatgataa
     2041 taaaaaaaca acaaccatta tatttcttat ttttttttct tttatcaatc ttttcaaaat
     2101 caatgatgca atcataaatg ataagaacac aagaaaaggt agtagtttta ttttccagcc
     2161 aacactattt gtctctgata tatttgctag tgcaagtgtg ggtatgtact cacctattga
     2221 tataatgctg cttatttcaa tagatataac catggatagt aatagaagta aaagcagaat
     2281 aattgctcta aatgcatctt ttatttttat attaagcatt ataatcataa ccgagaacat
     2341 ttgaataatt gaaattaaaa acatttataa aaaccttttt tttttggggg cgttgttgaa
     2401 taaatagata ttatgctgtg gatctacaaa aatgtcggtc ttcctcaacc taaaatcagt
     2461 aagttaagtt tagactcggg agcttatcac aatattgata agcgcatttg actaaataag
     2521 acatagaaaa cagcataatt ctccattatt caacaaatct ggagatggat tatacttttt
     2581 taattaactg tctgctagtg cggtgatgct tccaacttac tgatttagtg gatgacggtg
     2641 cttttgaggt gctccggtgg cttccatttc catcagctgt ccctcctgtt ccgctactga
     2701 cggcgttgtg cgtaacggta aaagtactgc cggacatcag cgctatctct gctctcactg
     2761 ccgtaaaaca tggcagctac agttcactta caccgcctct caacccggta cacaccagaa
     2821 aatcattgat atggccatga atggcgtcgg atgccgggca accgcccgct ttattggcgt
     2881 tggcctcaac acgattttcc gccatttaaa aaactcaggc cgcagtcggt aacctcgcgc
     2941 atacagccgg gcagggacgt catcgtctgc gcggaaatgg acgaacagtg gagctacgtc
     3001 ggggctaaat cacgccagcg ctggctgttt tacgcgtatg acagggtacg caggactgtc
     3061 gtggcccacg tttttggtga acgcacaatg ttcacgctgg agcgtcttct gagcctgcta
     3121 tcggcctttg acgtggtggt attgatgacg gacggatggc cgctttatga atcccgcctg
     3181 aaggaaaagc tgcacgttat cagcaagcga tatacgcagc gaattgagcg gcataatctg
     3241 aggcagcatc tggcaaggcc gggacggaag tcactgtcgt tctcaaaatc ggtggagctg
     3301 catgacaaag tcatcgggca ttatctaaac ataaaacact atcagtaagt tggattcatt
     3361 atcctaccta cctcttggca ggaaaaagcg ctcgcaaaaa cggtgagaaa aaaacaagcc
     3421 tatatcagat aacagccctc tcctagacag ggctgttact ttgccgtaac cacagaatct
     3481 gtcatactac actaccgcct aagtcagtat acactccgct agcgctacgt gactggttta
     3541 gggctgcgcc ccgaaacccg ctaaccacct ctcagctact ggatatcacc ccatgccatc
     3601 tgaacatgat gaacaactgg attcgactga tacgcagctt cccggcgaat atgacctttt
     3661 tgatttaatg gcgcaggtta ctgaagagaa cctgcacgcc ccggtggatt ttgggccgcc
     3721 tgtcggccgg gaagcgttat agctttttcg cttacccgcg tacgtggtaa tacgccggta
     3781 ttacatctta tttctttatt ttcatgtaat tacaggtggt tgtaatacgt tgcgtattac
     3841 ataaataaac ggggacttca gtttttacct tttaattcat gcaattgcga tgaaaatcgc
     3901 ggttgcgtat ggtgtatgac catttaaggg ataatgtaac ctgactttta aagaatgaat
     3961 ctatctttgg ctgcgcatga gatcgataaa aagcgttcca catcaggcgg ttcatggata
     4021 catgaaccgg aaaggacaac aataagacaa ttgtaagaca tttttttgcg tttcaatctt
     4081 atttttggac tattatgaga caaatttaag acattatggg cggtatatgg cgaattttaa
     4141 ccctggtcca agaacagcca ttgcggctgt cagggattcc aagaacgata tagaaacctg
     4201 gaagaaaaat ggtatccgta gggaagagat tgtgactgtg ctgaatgaac gttacggcct
     4261 gaatatctca ctggttgcgt ttgataaggc tctttacaga ttgagaaaaa gtacagatga
     4321 accgtctctt gctggtgtgg attctgtgtc tgtttcagaa acgaatttct ctgaagccag
     4381 tgggcctgac aatggttttt ttctgtccgg gaatgacttc atgcgagcag aagaagaatc
     4441 tcaaagagtg gttgagtcat tcagagatag ccttagcagg aagaaaggat tctgattttg
     4501 aattctaagt agtgaaatag tggatcctct actcgaaatt gtcggatacc tgcaattacc
     4561 taccgtgcct tacggcttgg tcaatgcctc acggccacgc aataaattgc gtccccgttc
     4621 accgttttcg gatgggaaga atgccggaaa caggacttac cctggctgaa tggggtgaat
     4681 tttcgcgcta tgcacttgcg tgcatactca tgcatgccgt aaaaacagag cctgcgcgtt
     4741 tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca gaaacgccct
     4801 gagagcctct caggcggtgc acgcaacgga tgttatggta aatccagctg attggcaggt
     4861 acaaaaaagc cccgcagggc ttttttgtac cttattgatc tgcacacttt gcagccttca
     4921 gggcttccag ctgatttagc cggttagctg cttttttgcg ggcttcgcct tttgccatgc
     4981 catttcctat cccaaaatca cccaggaaac ctaatacggt gcgcccgtca aattcgccgg
     5041 tttcctcaat ttcttgttgt acactgcgcg ttttggcgat ttccagagct accgctttgc
     5101 aatccattgc ggaagattct ggaccggtga cgttcgcggc ctgtggatat tgctttgttg
     5161 cgcatcctgc catgattagc gctgccgata ggatgaatat ttttttcaaa attatcctta
     5221 tttaggcata gttaagagat acccatttta tcggcaggaa atgagggttt tttagttgaa
     5281 ttttgggagg ggcaaggttc catattggtc gaggattctg gagctgaccg gcaacggcca
     5341 gcgcggctat gagccatgcg gtggttgagc atagaaagga tcttcttgag ttcctttttt
     5401 tctacgcgta atctcttgct ctgaaaacga aaaaaccacc tggggaggtg gtttttcgaa
     5461 ggttaagtca gttggggaac tgcttaacct ggtaactggc ttcagcagag cgcagatacc
     5521 aaatactgtc cttccagtgt agccgtagtt aggccaccac ttcaagaact ctatagcacc
     5581 acctacatta actcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgctttgt
     5641 cgtgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag cagtcggact
     5701 gaacggggga ttcgtgcata cagtccagct tggagcgaac tgccttcccg gaactgagtg
     5761 tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaacg
     5821 gcaggaacag gagagcgcac gagggagcca ccagggggaa acgcctggta tctttaagtc
     5881 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     5941 ggagcctatg gaaaaacggc tttgccgcgg ccctatcgct tccctgttaa gtattttcct
     6001 ggcatcttcc aggaaatctc cgccccgttc gtaagccatt tccgctcgcc gcagtcgaac
     6061 gaccgagcgt agcgagtcag tgagcgagga agcggaatat atcctgtatc acatattctg
     6121 ctgacgcact ggtgccgcct tttttgtcct gccacatgaa gcactttctc acagtggtgc
     6181 gaattttttc tcagcggtgc ctatggaaaa gcgaacgaaa gaaatcaaaa tccgcatgac
     6241 cgagactgag catcagcgtc tgcttgaacg gtgtgaccgt acagccagca actggagagc
     6301 ctgaaagccc agagcggcgc aggggtagcg atttacaagg acgacaagca gaagaacacc
     6361 tatctggtgg tactgccgaa gaaagcgcat ggcaccgaga cctatacatc gacagccgga
     6421 aacaccgtga tgaagtacac ctcgaaataa ccccgtcaga gcccacagaa aagccacagc
     6481 acgtttttat cactgaggac aggcaaaacc atgaccgaga tggaaaggca gcttctgagc
     6541 gccttagaaa gcttacaaac aagttatgaa caacagcaac aggcgtggca ggacagttac
     6601 agcagcttgc aacgcatgtt cgagactacc tcacaggcac tggagcgcaa cggcagggta
     6661 tgtctgaact tgagcaatca ggtgaacgcc ttgcacgaac aggtcgaacg cttgagcagc
     6721 gatctgagcc gattaacacg ctcgtaaagc agcaggaacg gcagttacag caggagaagc
     6781 aagcaacccg ctatcgaggt cagtccatgt accacacaag ccgtacttct cggatgattt
     6841 gcactttggt
//
