LOCUS       NZ_LR130551            13841 bp    DNA     linear   CON 26-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KPC2 isolate KPC2 plasmid 4.
ACCESSION   NZ_LR130551
VERSION     NZ_LR130551.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA5128438
            Assembly: GCF_900622645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Seemann,T. and Baines L,S.
  CONSRTM   Bioplatforms Australia Antibiotic Resistant Sepsis Pathogens
            Initiative
  TITLE     Direct Submission
  JOURNAL   Submitted (24-NOV-2018) Bioplatforms Australia, Level 4, Bldg F7B,
            Research Park Drive, Macquarie University, NSW, 2109, Australia,
            Australia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/26/2023 02:44:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,631
            CDSs (total)                      :: 5,506
            Genes (coding)                    :: 5,396
            CDSs (with protein)               :: 5,396
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 54 of 110
            Pseudo Genes (incomplete)         :: 62 of 110
            Pseudo Genes (internal stop)      :: 12 of 110
            Pseudo Genes (multiple problems)  :: 15 of 110
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LR130551.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPC2"
                     /isolate="KPC2"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="4"
     gene            1..558
                     /locus_tag="EW042_RS28650"
     CDS             1..558
                     /locus_tag="EW042_RS28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            792..1346
                     /gene="aac(6')-Ib"
                     /locus_tag="EW042_RS28655"
     ARG             793..1343
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1416..>1631
                     /locus_tag="EW042_RS28660"
                     /pseudo=""
     CDS             1416..>1631
                     /locus_tag="EW042_RS28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     gene            1697..2401
                     /locus_tag="EW042_RS28665"
     CDS             1697..2401
                     /locus_tag="EW042_RS28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <2456..2626
                     /locus_tag="EW042_RS28670"
                     /pseudo=""
     CDS             <2456..2626
                     /locus_tag="EW042_RS28670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            2742..3563
                     /locus_tag="EW042_RS28675"
     CDS             2742..3563
                     /locus_tag="EW042_RS28675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199321.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_004199321.1"
                     /translation="MSFRSTERQTPTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDK
                     AKAELDAEDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEY
                     GSGWQDQVRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKD
                     AENKLNEEKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYG
                     DKKRKIYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            3573..3830
                     /locus_tag="EW042_RS28680"
     CDS             3573..3830
                     /locus_tag="EW042_RS28680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            3915..4064
                     /locus_tag="EW042_RS28685"
     CDS             3915..4064
                     /locus_tag="EW042_RS28685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            5545..7509
                     /locus_tag="EW042_RS28695"
     CDS             5545..7509
                     /locus_tag="EW042_RS28695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            7509..8240
                     /locus_tag="EW042_RS28700"
     CDS             7509..8240
                     /locus_tag="EW042_RS28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            8247..8777
                     /locus_tag="EW042_RS28705"
     CDS             8247..8777
                     /locus_tag="EW042_RS28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8805..8984)
                     /locus_tag="EW042_RS28710"
     CDS             complement(8805..8984)
                     /locus_tag="EW042_RS28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9053..9430
                     /locus_tag="EW042_RS28715"
     CDS             9053..9430
                     /locus_tag="EW042_RS28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(9492..9791)
                     /locus_tag="EW042_RS28720"
     CDS             complement(9492..9791)
                     /locus_tag="EW042_RS28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9781..10044)
                     /locus_tag="EW042_RS28725"
     CDS             complement(9781..10044)
                     /locus_tag="EW042_RS28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(10350..>10640)
                     /locus_tag="EW042_RS28730"
                     /pseudo=""
     CDS             complement(10350..>10640)
                     /locus_tag="EW042_RS28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(10675..13680)
                     /locus_tag="EW042_RS28735"
     CDS             complement(10675..13680)
                     /locus_tag="EW042_RS28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143775.1"
                     /translation="MPVDFLTTEQVESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHDEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISQSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLGENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASDLVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     ARG             1429..1685
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             742..1346
                     /name="AAC(6')-Ib10"
                     /gene="AAC(6')-Ib10"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.97"
                     /coverage="99.01"
     Typing          complement(4168..4746)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7510..8237
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 atgcgaattt ttggttatgc gcgggtctca accagccagc agtccctcga tattcagatc
       61 agagcgctca aagatgcagg ggtaaaagct aaccgcatct ttaccgacaa ggcatccggc
      121 agttcaacag atcgggaagg gctggatttg ctgaggatga aggtggagga aggtgatgtc
      181 attctggtga agaagctcga ccgtcttggc cgcgacaccg ccgacatgat ccaactgata
      241 aaagagtttg atgctcaggg tgtagcggtt cggtttattg acgacgggat cagtaccgac
      301 ggtgatatgg ggcaaatggt ggtcaccatc ctgtcggctg tggcacaggc tgaacgccgg
      361 aggatcctag agcgcacgaa tgagggccga caggaagcaa agctgaaagg aatcaaattt
      421 ggccgcaggc gtaccgtgga caggaacgtc gtgctgacgc ttcatcagaa gggcactggt
      481 gcaacggaaa ttgctcatca gctcagtatt gcccgctcca cggtttataa aattcttgaa
      541 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      601 cttagacgtc aggtggcact tttcggggaa aagtgcgcgg aacccctatt tgtttatttt
      661 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      721 aatattgaaa aaggaagagt atgagtattc aacatttcca aacaaagtta ggcatcacaa
      781 agtacagcat cgtgaccaac agcaacgatt ccgtcacact gcgcctcatg actgagcatg
      841 accttgcgat gctctatgag tggctaaatc gatctcatat cgtcgagtgg tggggcggag
      901 aagaagcacg cccgacactt gctgacgtac aggaacagta cttgccaagc gttttagcgc
      961 aagagtccgt cactccatac attgcaatgc tgaatggaga gccgattggg tatgcccagt
     1021 cgtacgttgc tcttggaagc ggggacggat ggtgggaaga agaaaccgat ccaggagtac
     1081 gcggaataga ccagttactg gcgaatgcat cacaactggg caaaggcttg ggaaccaagc
     1141 tggttcgagc tctggttgag ttgctgttca atgatcccga ggtcaccaag atccaaacgg
     1201 acccgtcgcc gagcaacttg cgagcgatcc gatgctacga gaaagcgggg tttgagaggc
     1261 aaggtaccgt aaccacccca gatggtccag ccgtgtacat ggttcaaaca cgccaggcat
     1321 tcgagcgaac acgcagtgtt gcctaaccct tccatcgagg gggacgtcca agggctggcg
     1381 cccttggccg cccctcatgt caaacgttaa acatcatgag ggaagcggtg atcgccgaag
     1441 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1501 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1561 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1621 accttttgga aacggcactg ttgcaaatag tcggtggtga taaacttatc atcccctttt
     1681 gctgatggag ctgcacatga acccattcaa aggccggcat tttcagcgtg acatcattct
     1741 gtgggccgta cgctggtact gcaaatacgg catcagttac cgtgagctgc aggagatgct
     1801 ggctgaacgc ggagtgaatg tcgatcactc cacgatttac cgctgggttc agcgttatgc
     1861 gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac ccttccgatc tttgcccgtg
     1921 gcacatggat gaaacctacg tgaaggtcaa tggccgctgg gcgtatctgt accgggccgt
     1981 cgacagccgg ggccgcactg tcgattttta tctctcctcc cgtcgtaaca gcaaagctgc
     2041 ataccggttt ctgggtaaaa tcctcaacaa cgtgaagaag tggcagatcc cgcgattcat
     2101 caacacggat aaagcgcccg cctatggtcg cgcgcttgct ctgctcaaac gcgaaggccg
     2161 gtgcccgtct gacgttgaac accgacagat taagtaccgg aacaacgtga ttgaatgcga
     2221 tcatggcaaa ctgaaacgga taatcggcgc cacgctggga tttaaatcca tgaagacggc
     2281 ttacgccacc atcaaaggta ttgaggtgat gcgtgcacta cgcaaaggcc aggcctcagc
     2341 attttattat ggtgatcccc tgggcgaaat gcgcctggta agcagagttt ttgaaatgta
     2401 aggcctttga ataagacaaa aggctgcctc atcgctaact ttgcaacagt gccctgataa
     2461 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2521 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2581 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2641 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2701 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2761 agcgtcagac ccctactccg gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc
     2821 agcaggcatg ggacgctgct cacccggaag aggggctgaa aagagagtat gataaagcga
     2881 aagctgagct ggatgctgaa gataaaaata ttgcgacctt aaacagtcgc attgcatcga
     2941 cagagaaggc gatccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag
     3001 aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg
     3061 ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa
     3121 aagctgccca gacatcttta aacgaaatga atgaatcctt atccagggat aaggctgcgc
     3181 tttccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata
     3241 aattaaatga ggaaaagaaa aaacctcgta agggaactaa agattacggc catgattatt
     3301 ttcctgatcc caagactgaa gatattaagg ggttgggaga gttgaaagag ggtaaaccta
     3361 aaacccctaa acaaggtggt ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta
     3421 aaatttatga atgggattcc cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg
     3481 aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac
     3541 gaaacattaa aaaatatctt taagaggtaa atatggggct taaattacat attcattggt
     3601 ttgataagaa aaccgaagag tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg
     3661 gttctgtcat tgaaagtctg gggatgcctt taaaggataa tattaataat ggttggtttg
     3721 atgttgaaaa atcatgggtt tcgatattac agccacactt taaaaatgta atcgatatta
     3781 gtaaatttga ttactttgta tcctttgttt accgggatgg taactggtaa gcaaaagtta
     3841 tcaaggatga tttgaaatac agacttccgg tgttcaggga tgaataccgg ggtttttaaa
     3901 tacagggaag gtatatgaaa aaggtaaagg ttgtttttct ttttatattg attttttcag
     3961 gctttcttct ggtcgcatgt caggcaaact atatccggga tgttcagggt ggaacggtgg
     4021 caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc
     4081 ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg
     4141 gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc
     4201 tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact
     4261 tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc
     4321 tgctgccagt ggcgttttgc gtgcttttcc gggttggact caagtgaaca gttaccggat
     4381 aaggcgcagc agtcgggctg aacggggggt tcttgcttac agcccagctt ggagcgaacg
     4441 acctacaccg agccgagata ccagagtgtg agctatgaga aagcgccaca cttcccgcaa
     4501 gggagaaagg cggaacaggt atccgggaaa cggcagggtt ggaacaggag agcgcaagag
     4561 ggagcgattc atcggaaacg gtgatgatct ttaagtcctg tcgggtttcg ccactcctga
     4621 ctgattcatg gttgagccac cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg
     4681 aactcagtca ggagggcgga gcctatggaa aaacgccacc tgcgtggcct tgccgttttg
     4741 cctcacatgt tagtcccctg cttattcacg gaatctgtgg gtaactttgc atgtgtccgc
     4801 agcgcccgcc gcagtctcac ggccggagcg tagcgaccga gtgagcgagg aggcgcaatt
     4861 tccccggcac ctacaagacg gacaccggac gcgctgatgt ggcatattgt cccctgatta
     4921 agtctttata ttccctgata ataaattacc aggataggtg aatcatatcg gtttttcgct
     4981 gaacatttta cgttaaccta tctctgttgc ttttttctcc ttacggagaa atacaggtca
     5041 tccccgcgag ggcgtggctg acctcagggg gatatcccct gcttacgctg gctggacgcc
     5101 accgacgcac ggtctctccc cctctttctg gctccgtccg gctggacgcc ggccacgcca
     5161 gaaattcacc cggcccccgt caggctcacg cctgaccacc cacaaaaatc cacggtgctg
     5221 gacgcacctg gcgaagcagc aaagctgctc ctgattttgc gggtggtcag ccgctcgcta
     5281 tggcgaactc tgacgggggc cggaacaccg gaaaagaccg cactggggtg ccgccttttc
     5341 ctctcacacg ctggggcgtg ctcgttgttc ctccggggct gcgccttttc cgcatggggc
     5401 ggaaagaggc tggcctccgt ggtgaaagct gaacgcaatc accacggacg tgaccgccgc
     5461 cgccatgcct tggggcatgt ccgcggcttg tgcagcgggg gctgcacatc acgccggcaa
     5521 accatcgtct gaaggagaga gaacatgttt aatacggatt cgcttgcctg gaagtggcac
     5581 attccggagc tggcccggca gatttatccg tgggtcagct ggctgtacca ggagtattac
     5641 ggtctggccg gggctttcct agttatcggc ctgtttttcg gtctggctga actttgggcc
     5701 ggacggatgc gttttgtggg gctgagtcat aaggagcgac gggagtatgc cgatcgctat
     5761 aagcgcgata ataaacggcc agcgccattt tcggtgctgg cctcgctgga ttcgcccggg
     5821 cggatggtca gggcacgtcg acatgtctgg aactacgcgt actggctgac cggcgcggcc
     5881 ggtctggccc ttctgggggg gctggttgtc gccgtgttta tcgagagctg gggagaaacc
     5941 gtcctgcgtc cggacctcta cgcaaacgca gattattacc tgctgcagcc ggctaacctg
     6001 ttcgggattg cctggtgcgc gggcgggctg gctgtcggga ttgtgttggc gcggctgctg
     6061 gcgttcctgc tgcttgacgg tcattttgcg gctgctgacg aggcggtgaa cgcgaaactg
     6121 aatcaggaga gccggcgcag cagccagcgg acaggagaga tgaccgacgt gcgccacctg
     6181 catttcgggg agccggtacc ggttaatgcg ctggccgatt tttcctcgga gcaggcccgg
     6241 caggagcagg cggtgtttct gggtaaggat gagcagggca agccggtgct ggttccgcgt
     6301 gatacctggc gcaagaccaa cattcagatc ctcggcctgc ccgggagcgg caagagcgtg
     6361 atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgcggt ggtgtacttc
     6421 gaccccaaag gggacgcatg ggcgccgcac gtcttccggg cgcactgtcc ggacttcacc
     6481 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag
     6541 tacgcgctga aaaacctgct gattgccggg cttaacctgg gcgagaccgg cgacgtggcg
     6601 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gtttccgcag
     6661 ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgc tgccggaggc cctgaaaaag
     6721 gacgtgaagg ggttgatcac taggctggaa aacgtcgctg acctgagcgt cctgcagacg
     6781 gacagtggca tcgatgtggc ggggattatc aacggcggcg gctgcctgta cgttatcggc
     6841 tcgatggacg acgaggcggt gatccgtgtg caaaaaatgc tgtttgcccg ctgcgcgcaa
     6901 atcatcattg cgcgggacga gttccgggag tggccacacg tcagcgtgat gctcgatgaa
     6961 attaaatatc tgctgtcgaa gtatgtactg aacgcactgg ggacgctgcg cagccgggac
     7021 tgcaatctgt tgctcgcgca ccagtcgctg ggcgatttcg ggcagtgcgg gcaggatctg
     7081 cccgctgaat ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttttaccgg
     7141 gcggccagcc tggagtccgc gcagtgggcg gcggggcaga ccggggaaat cagggtggac
     7201 gtggagcgcc gccgtgccag ccgggaggcg gggaacgtgg agcatatcag cggggacagc
     7261 ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgccggac
     7321 gggttcgcgg tcatgaccgg gctgggtgtg gcccggatgg cgttcagcag tccgctgcgg
     7381 gttgagcgcc gggaaatccc gttgcagcgt ttcccggtgc tggcgaaaac cgatcccctg
     7441 gcggagttcc ggcccccggc cgacggcggc gggccgaaag cggatgatga ttttgcgggg
     7501 atttactgat ggcgctggag ctgtacaacg tcagccacgc gaaacgacag gcgcggaacg
     7561 cggagaaaac ccggctgacc ctgcgctggt tgcgggaaga gctgtgttcc acggcggagc
     7621 tggttgcgcg gcggctgggg attgcggcgg tgcagccggt ctaccgcttc ctcgacagcc
     7681 tggtggcgaa agggctgctg gtccggacca aatatccggt ggacggccgt caggtcagcg
     7741 tgtgggggct gacgccgcat ggcgtggcgt tctcgttcga cgaggacgaa ccgctggcgg
     7801 atgttattcc gtttcagccg tcaagggtgt cggcagcaca tctgccgcac cggctggccg
     7861 tgcaatccct gcgccttgat atggaagcca gaggggccac cggctggcgc attctgcacc
     7921 gtctgagtct taaaggcatg aaagtgccgg acgccctggc ggagctggac ggtaaaacgg
     7981 tggcgtttga ggtggagcgc accgtcaaga gccgtcgccg gtatcaggaa gtggtgtccg
     8041 ggtatctgtt caaccgcaaa gctaacggca tcgatgaaat ctggtacatc tgcccggacc
     8101 gggcgacgca ggtcagggtg cagcgggcaa tcctgtcggt ggatgaaatc gtgaacccac
     8161 agaccggcga ggcccgcaaa acggcagagc tggatcgtga acggttgttt gcgtgcttta
     8221 aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt gaagacgcgg
     8281 aggaccggaa cggcgagcgc tgcatcatgc cgaccagcga gcatctggaa gatatggtgg
     8341 tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg
     8401 cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt
     8461 actttttcac tggtaaaaat ccgcaatggg aacggaacaa acccacagga atgaagctgt
     8521 gtgattacct ctatcgcaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg
     8581 atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgacgtggtg agtgagcggc
     8641 acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc
     8701 gctattttaa cgtgccggta cctgaactgc cggaggacaa acctgcccgc cggtggtggc
     8761 cgttcggcaa aaaataaatg ccagcaccgg ccatgccggt gctgttattc ctccccgagc
     8821 ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gtagccgctc gcacatgtcg
     8881 gccggggtat ccatatccag ctcattcagt ttatcgagca atagcagcga ctgctcctta
     8941 ataaatttag ccatattgag ggctgttttt tcctgttttg tcatgattct ttccttccgg
     9001 gcatggtgtg cgcctgtcag gtatactgtc tcaaaaatag cggggtgaga caatggcgtg
     9061 ggtgacggtg agacaggcga cggaactgac gggtaaagcc cgaagttccc tgtatcggga
     9121 tatggcgaaa ggccgcgtat cataccgtac ggaagctgat ggcggccggg tggtggatac
     9181 cagcgagctt atccgggtgt acggtgagtt aagacaggat gagactcacg agcgggacga
     9241 gttgagactg tcgggtgaga ctgaaaaaat ggcctccgac gcgttgatcg cggagataaa
     9301 agcactcagg gaggaggtgg ccgggctacg gcaggaaatg cagtccatga gactgctgga
     9361 acacaaaccg gagacagcag agaccccggc cggaacaccg cgccggtggt ggccgtgggg
     9421 aaaacgctga cgcgctgtgc ttgtccaacc ggcgacggcc acgcaatgaa ttgcgtcccc
     9481 gtcaccggtt attagagaac aatatgggga ttctggcgct ggccgaccac ggccagaacc
     9541 cggacttttc tggtctggtc atcaacatca tagaggacat gctgtcccat cagactgatc
     9601 cggcgaacgt cctcgccata gcgtgagtcc acttcataga gtcgcgggaa gtcggccagt
     9661 ttgtccgccg cagcgagaac ccggtcggta acgttctgcg catcccacag ggatgaattt
     9721 tccgcgatat agtcaaacag cgtcgttaca tcctcgatgg ctccctgctt ccattccacg
     9781 tcatacggca tcgcgcagtt cctgataacg gccatgcagg cgctggcgga gttgttccgt
     9841 ggtcataccc ggacgcgtgt cagcacgggc ggcggtcact ttggcgcgaa cctgatcttc
     9901 atactctgcc tgggccagtt cttcgttagc cagtcgctgc aacagcgcag ctttgtccgg
     9961 gccaccttcc ggcagtatgc ggggcaggct ttcagccagt tcccgaagaa agttggcgtt
    10021 ttcgtagtgt gtagtgttgc tcattgcgga gcctcccggg ttaatgcggc aatatgaagg
    10081 aagtttagca gggggcggtc tgaaagaata tggtcgtgtc agtcaggctg atgaagcaga
    10141 aagtcggtaa aaaagggctt acggcatcca ttttacgtca aaacatatgc catgcagttg
    10201 cgtgcatagg catgcattag gataaaattt atcgggcgcg tttccggcag ttttccgggg
    10261 gggttgttgc ctgttttatc ccgtagccgc cggaaacgcc ctgagcccgt ctgagcggtg
    10321 cgcgtaatga cgcgttatgg taaatccact tactgtctgc cttcatagcc atcgagataa
    10381 acagctgaaa tggtgtcagc cagtagcctt gattttttcg gtgtgaggtc tttgctgcgg
    10441 taatcggtac ccgcaaattg tgttgtgcct tggagtgagt ttaaaaattc ctgactgtga
    10501 aactgctgaa tgcgttgctg cacatcactc cgtgattttc cggtcgcttt atcctttgcg
    10561 cccatatccc agaactgccg gaaaaaaggc accatcattc gtgttgtgtc agctttctgg
    10621 gtggaaaaat tgggatcaaa aggggtctga cgctcagtgg aacgaaaact cacgttaagc
    10681 aacgttttct gcctctgacg cctcttttaa tggtctcaga tgacctttgg tcaccagttc
    10741 tgccagcgtg aaggaataat ggccgagcat attgatatgt ccgtggcaaa gcggggagag
    10801 gcgtgcgata tcttcatcat tcagtgtttc accctgcgcc cggagatgat ccagagctgc
    10861 ctgcatataa atagtgttcc ataacacgac ggcgttagtg accagcccca gagctcccaa
    10921 ctgatcttcc tgaccgtcgg tatatcgttt tcttatctca cctttttgac cgtgacagat
    10981 ggctctggca actgcatgac ggctttctcc ccgattaagc tgggtcagaa tgcgccggcg
    11041 gtaatcttca tcatcaatat aattaagcag atacagcgtt ttgttgatgc gccccacttc
    11101 aatgattgcc tgagtcagtc cggagggacg ttcacttttc agcaatgaac ggaccagcac
    11161 tgaggcctgt actttgccca gcttcaggga gcctgcggtc cggatcattt cgtcccactg
    11221 aaggactatt tttcggggat ctgattgccc tctggcaata tcattcagca cgccatagtc
    11281 ggcatcatgg tccattcgcc agaaaaccga agcaccggca tcagccaggc gtggagaaaa
    11341 ctggtatccc agcagccaga aaaggccaaa gacaagatcg ctggcacctg ccgtatcggt
    11401 cataatttcg gttggattca gcccggtctc ctgttccaga aggccttcca gcacaaagat
    11461 agagtccctc agcgtccccg gtataacgat gccatgaaag ccggaatact gatcggacac
    11521 aaagttgtac caggtgatcc ctctgttatt accaaagtat ttgcggttcg gtccggcatt
    11581 gattgttctg actggcgtaa caaagcgcat tccatctgca gatgccactt ctcctccacc
    11641 ccatatctgt gccagtggca gcgttgcctg aaaatcaacc agtctggcat tagcgctggt
    11701 gatagtttca gcccgcagat agttcgcttt tgtccagttc agccggtgtc gggtcagtgc
    11761 aggaacattt gatctgatca gtggttccag accgatattg caggcttcag ccatcagcac
    11821 ggcgctgatg ctgacgggca gatcatcaac tctggcactg gcttcactgg catggaaaaa
    11881 ctcatcagca aatccggtat gggcgttaat ttcgagcagc aactccgtta aatccaccgg
    11941 agggagcaga tcactgatca ttttgctcag tcgtttcaga ctgtccggct catcaagact
    12001 ggcgagggga gaaattgtca accggggctt cgggccagaa acatcgagtt cgacagcctc
    12061 attttcgcca agacgtgcag caacctgtct gtaacgacta tcaagctgat ggcccagaga
    12121 ttttattgct tcctgcgggt ctgtcgggtg ccccaaagaa cgataaacct taatccgatt
    12181 tgcctgccag tcagcaccct gtagtaatct tgcacgagga tctccccacc ggttactgcc
    12241 ggtaacgtag acatccctcc gtctcagact atcctgcagt ttactgagaa agcagagcgt
    12301 gtatcccctt cgggtgatat gtttttcctt gttaatcacc agccgtttcc atgactgact
    12361 gatgatttcc gttggtgcgt cgtcaaaaaa ctgccgccgt gagctgaact cccggctgag
    12421 gtagtcacag gcattcagag tggtaacccc ggcaggtgcg gatgaaaatt taacggtatt
    12481 cagcagatgg ggcaggaaac gacgaacgcg cccgtactgc tccaccattt cgtcatgaaa
    12541 attatcgtct gagggccggg caatttcacg gacaagcgtg atgatttcag ccagcttttg
    12601 cctagggatg tagctgaaca cctcagcacg aatcgattcg tccggtgttt cttctttcag
    12661 caagtacgaa catgcgctgg cgagcgccaa tgcagattta tccagatcct tcagcgagcg
    12721 gagccgtttt ttctgcccaa tctttctggc gtcacggatg ataacggcca gcatggcgtc
    12781 cagaacgtcc agtgcatcat ccagcgccag cgtttcccat gcaaggacaa aggcaaccag
    12841 aaccgccatc cttttctgcg gtgacatcct ggcaatattg aacaccgaag tcataccagc
    12901 ataacgtgcg agatttttca ggcgcacagc cgggagtgta ctcaggtttt cagcatgcag
    12961 gccaaaatcg ttcagagttt tccagcgttc aattgcttca ttaaacgccg gaccactgat
    13021 ggtcacaggg ccttttttca gtgattccag taaagacagg cggctgcaat cagttggccc
    13081 cagcagcatc tccagctgtg aacgctgttc ggctgacggt atcagtgcca gtttgttcca
    13141 caggcgcaac gtcgcctttt cccttacctc tgaaatcaac cgggtcagcg tggtggctcc
    13201 ggggagaata atacgatgtt gcataagcca ccctgtcgcc aggtcgaaaa gcaggccagg
    13261 acgttcgttg cttatccagc tccgggtata taaaagacgg gtaaggcgaa atgtccaggg
    13321 ccaggcaaat tcacgatact gatagtgctg acgtatcagc gctgcatgct cacggcgggt
    13381 attttccctc tgaccgtatt ctgcaagaac ggtgatatca cgaatcccga gctgtctggc
    13441 ggtaaaatgc cggacgccgg aaggaatatg attcatgtca gtaagaaaag tgcccagaaa
    13501 acgcacacac ccgatttgca gggcaatacc aagtcgattg tgatcacccc ggcttttccc
    13561 gataaattct ttatccgctt catcaagatg aaaataacgc gccagctgaa gttcatcggg
    13621 ttcgccagtg aacctgccat aactctcaac ctgctcagtg gtcaaaaaat caacgggcat
    13681 atcggcctcc ctgcctgacg gcttttttaa cacaactgca accgttcgaa atattataaa
    13741 ttatcagaca tagtaaaacg gcttcgtttg agtgtccatt aaatcgtcat tttggcataa
    13801 tagacacatc gtgtctgata ttcgatttaa ggtacatttt t
//
