LOCUS       NZ_LC543864            10060 bp    DNA     circular CON 12-JAN-2024
DEFINITION  Serratia marcescens strain Sm4 plasmid pSm4Col, complete sequence.
ACCESSION   NZ_LC543864
VERSION     NZ_LC543864.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Serratia marcescens
  ORGANISM  Serratia marcescens
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1
  AUTHORS   Hayashi,W., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     TEM-61-producing Serratia marcescens isolates causing
            healthcare-associated infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Hayashi,W., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-APR-2020) Contact:Wataru Hayashi Shinshu University
            Graduate School of Medicine, Science and Technology, Medical
            Sciences, Health and Medical Science Unit; 3-1-1 Asahi, Matsumoto,
            Nagano 390-8621, Japan
COMMENT     ##Assembly-Data-START##
            Sequencing Technology        :: illumina Novaseq6000
            Assembly Method              :: A5-miSeq pipeline v. 20160825
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/12/2024 02:29:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LC543864.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Serratia marcescens"
                     /mol_type="genomic DNA"
                     /strain="Sm4"
                     /isolation_source="pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:615"
                     /plasmid="pSm4Col"
                     /geo_loc_name="Japan"
                     /collection_date="2009"
     gene            complement(1..258)
                     /locus_tag="I0P76_RS00005"
     CDS             complement(1..258)
                     /locus_tag="I0P76_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(268..1953)
                     /locus_tag="I0P76_RS00010"
     CDS             complement(268..1953)
                     /locus_tag="I0P76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            2280..2525
                     /locus_tag="I0P76_RS00015"
     CDS             2280..2525
                     /locus_tag="I0P76_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2799..3170
                     /gene="tolA"
                     /locus_tag="I0P76_RS00020"
     CDS             2799..3170
                     /gene="tolA"
                     /locus_tag="I0P76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3167..3532
                     /locus_tag="I0P76_RS00070"
     CDS             3167..3532
                     /locus_tag="I0P76_RS00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3707..3985
                     /locus_tag="I0P76_RS00030"
     CDS             3707..3985
                     /locus_tag="I0P76_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            3973..4287
                     /locus_tag="I0P76_RS00035"
     CDS             3973..4287
                     /locus_tag="I0P76_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4451..4879
                     /locus_tag="I0P76_RS00040"
     CDS             4451..4879
                     /locus_tag="I0P76_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            4905..5084
                     /locus_tag="I0P76_RS00045"
     CDS             4905..5084
                     /locus_tag="I0P76_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5111..5641)
                     /locus_tag="I0P76_RS00050"
     CDS             complement(5111..5641)
                     /locus_tag="I0P76_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5648..6379)
                     /gene="mobC"
                     /locus_tag="I0P76_RS00055"
     CDS             complement(5648..6379)
                     /gene="mobC"
                     /locus_tag="I0P76_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6379..8343)
                     /locus_tag="I0P76_RS00060"
     CDS             complement(6379..8343)
                     /locus_tag="I0P76_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(9827..9976)
                     /locus_tag="I0P76_RS00065"
     CDS             complement(9827..9976)
                     /locus_tag="I0P76_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..6953
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9146..9724
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5652..6379)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ttaccagtta ccgtcccgat aatcaaaagc tatataacaa ttatccacat caggttttat
       61 ttgattttta aaaaatggct gtagtaaagg aacccattct ttcttcaaat caaactcacc
      121 attattaata ttatccttta aaggcattcc caagctttca atgacagaac catcgtcacc
      181 aaagtcttta gaatactctt cccctttaaa ctcttcagtt ttcttatcaa accatgtcaa
      241 acgtaattta agccccatat ttacctctca aagatatttt ttaatgttcc gttttggatc
      301 tggcccttta acctgcttac ccgttttggg atcaaatgcg cctaggtgtt cgccatcact
      361 ggcacgatac ccttcaagct caccgtgctg ggaatcccat tcataaattt tacgtttttt
      421 atcgccatac catcgggccc gcttaccacc accaccttgt ttaggtgttt taggatcacc
      481 tcgctttaac tctccaagtc cctttatgtc ttctgtttta ggtactggat gataatcatg
      541 gccgtagtct ttagtcccct tgcgaggctt gttcttttcc tcatttaact tattttctgc
      601 atccttcgct tttttctcct tttgtttccg gctctccatc gccccggtaa gtgcagcctt
      661 atctctggat aaggattcat tcattgcgtt taaagatgcc tgagccgctt ttaatttctc
      721 attctgattc tgaatatcct tatcaagata gcgaacctga tcctgccacc cggagccata
      781 ttcatgaggc gggttataag tcacaaaatc atctttattt gcctctgcct cttttacctt
      841 tttatcggct tcctgaacag cagcccttgc accggggagc gccttctctg tcgatgcaat
      901 gcggctgttt aaggtcgcaa tatttttatc ttcggcatcc agctcagctt tcgctttatc
      961 atagtctctt ttcagcccct cttccggatg agcggcgtcc catgcctgct ggcgacgctt
     1021 ttcttcctcc tgacgctgtt ttacctgtgc cggggtaaga acatctgtca ccgacacata
     1081 aaccggcttc tgtccggtct ctttcgggaa acgaataacg gcctcacggg agttaccacc
     1141 ggctgtaaaa ccagccggac gtgaatcacc tttttcagca acaatgcctt ttggcggggc
     1201 tttcgctgtc ggaacacctt tcggtacgct cacctgcaga gacgggagac ccggaatcga
     1261 cacactgaat acccccggac gttttgtcgg tttcgcatcc accacaggga cactcattgg
     1321 ccgacctgcg acaaccgcaa tatgctgccg ctcatccttc accacatcca caacccgttg
     1381 tctgacacga accgtcgcct ggtccagtgg taaagtactt gccggagtct ccgtcaccgc
     1441 atcggccggt aatgacgtca taatttttga catcattttc ggatcatctt ttgcaatctc
     1501 agaaggcagc acaccgtaga tcgcaatccc ccacagacca aacttaaacg gccccttcag
     1561 ggcagccagc acatcagcaa cggcggccga caacgcatcg ccggaaactg ataaagccag
     1621 tccttcagcc cccggagtag ccagggcagg cagaccgaag gccatcgcag tggccgaaga
     1681 ctgtccgcca ccagagctac cgctatgatt gccgctgtta ccaccattac caccgggttt
     1741 actcccgccg ttaccaaact cccccggatt atagttatga atatctccat tcggcgtatg
     1801 cgtcgtaccc cagcccggcc cgctgtttgg atccgtacca ccacctgatg aagggccacc
     1861 tttaccagac gtaccgttca cattcccact ggcctgacca ccattatgtc ccagacctga
     1921 attacccgga cctcgaccgt ctccaccact cataccgttt tcctctttga catttaaaac
     1981 aataaattaa agccaacata ctgtacatat acacagtata attattacct tactcctccc
     2041 gttttcaggt gtcaatatat gagattagtt ctcatcttca ggcacaaaaa agcgcccgga
     2101 gccacctgta agcagatcgc gtcttttccc gaaaatttct catggcaaca aattttatta
     2161 tttaagacat tcatcacact tacatttcag atggtataga cacatctgaa agctaactgg
     2221 catgaggaga ggacctgcgg ttaaactgtt cgggaaacct gagacgcccg gaggccacta
     2281 tgacgcacct gaaactgact cccgctgaag aaaagcgcct ggcggacgcc tttgtcgctc
     2341 aggcactggc catgggcctg aaagggaaca aaacgctgcc aaaggcggta aaacaacagg
     2401 tgcgggaaag ggtgcttcgt gccattcagg acgaacgtga agccaggcgg gcgcaggaaa
     2461 tgcgtcggga gaaatacgag gctacggaaa ccaccttcac ctggcagcca cagcgccgcc
     2521 ggtaaggggt atttaccata acgtcgcatt gcccgcaccg ctcagacggt ctgagggcgt
     2581 ttccggcggc tacgggataa aacaggcaac aaaccacccg gaaaaccgcc ggaaacgcgc
     2641 ccggtaaatt gtaacccgct gcatgcctat gcacgcaact gcatggcggg ccgtttggtg
     2701 taaaaacgat acgataagcc gtgtttatgt aaatgaacgt ggccgtaaag agtgacaaat
     2761 atacatgtaa cattttttac tgacaggtca tcaacccgat gcgtttaata ccctttatcc
     2821 tgctggcatt gtgcagcccc ttactcctga cggcctgtaa gcattccacc acccagccgc
     2881 cgaaaattta tactctggat aagtcatcta aaatcagtag ctacgctgcg gccatcaaga
     2941 ccgctgttga aatgcagatg tttgaaccag aggcctatgc gggaaaaatg tgcaccgtgc
     3001 ggatatccat acaacgtgat ggcactctga acagcgccat tgctgaagga ggagatccga
     3061 aactctgtaa agcagcaata tcagccgtta cccacgccaa aattccgccg gcaccggatg
     3121 aaaatacctg gcaaatattc aaaaacgccc ctctggattt cagaccatga aagcttttac
     3181 tctgacagtg ctggcggcca tgttcctcac tccggtacat gcgataccac taaaacccgt
     3241 aaccacactg cagccaacct acccggtaca ggccgcagca aaacgcatcg aaggtcaggt
     3301 gaccgccacg tttgatattc tggacgatgg acgagtggac aacgtgtcgc ttaccggctg
     3361 tcaccttttt tttcgggaaa cgctgacggc aatgaggcac tggctatatc ccgcagggca
     3421 cccggcaatg cgacaggtta taaattttga attcagtctg aaaaatgacg gcttacagga
     3481 tccgcgacca gtcaccaccc aaaagctcaa tgatgatggt tgccgccagt aacaggtcct
     3541 ttaacccgca ggctgcaata gtcctggatg atgagtgagc ctgtagcagc cctgattagt
     3601 ggaagcgaag ctgaaaaatg ggcagcaaaa gccgtatttc tggcaaatag cgccgccacg
     3661 gattaaagta gtcatgtgta atacatatga ctacaaaggg gcttttatgt catcagcaac
     3721 atccatcagg ctcgatgaag aacttaagga ccggctgaaa acgctggccg atgaccggca
     3781 ccgctcagcg cacgcgctaa tgctggaggc aatcaccgaa tacatcgacc gggaagagaa
     3841 gcgcagccag tacctgcggg acggacaggc cgcatggcag cactatcagg aaacgggtct
     3901 gcaccttact gcggaagaag cggaagcctg gataagtacc tggggaactg agaacgagca
     3961 ggatgctccg ccatgccaca ggtaacgatt tccgcgctgg cacagcgcga tttacaacgc
     4021 cttcaggact ttctcaaaac caaaaatcgg ctggcagcca gaaaaggagg tgaggtgatc
     4081 gtccgggcta tccagcaact gaaaacattg ccagacattg gccgcccggt gccgtttctg
     4141 ccgctggaat ataaggaact ggtgatcggg tttggcgaca gtggctatgt gatgctctac
     4201 cgccacgacc gggaaatgga ccagattgtg atcgtcaccg tcaggcatca gaaagaatcc
     4261 gggtatccgg gggcagacag cctctaataa ccgatgacgg gggcgcaatt cattgcgtgg
     4321 ccgtcgccgg tttgacaagc gcagcgcgtc agaaaaccac atcctgaacg gaaaaacggg
     4381 taaaattcag cgatacattt accgtgagaa attacacacc tttcacaccc gataaaatca
     4441 ggggtataca gtggcgaaaa cattgctcaa cctttcacag gcagcacaag ccgcgggcat
     4501 taccagaaga acgctataca atcatgtaaa acagggaaag gttaccgtgt cacgtgacgg
     4561 gaaaaacaac cctgtcgtgg atgtgtcaga actgatccgt gtatacggaa atgtgaatat
     4621 ccctgagaaa caaattcccg gcatttcaca ccgtgaaaat acacaaaaaa atttcccaca
     4681 ggaacagctt ctggcaatgc aaaaggaact ggccgatctc cggcaggctg tcaccctgat
     4741 gctggaagat aaaacatccc gggaagaaga acgccgccag cacgatgatg agcgccggaa
     4801 actccaggcc gaagtggaca gattgacaac agaactgaca cagaagaaaa aacgcttctg
     4861 gtccggctgg tttagctgag aacttaccga agggaatgga gaacatgacg aagcaggaaa
     4921 aaactgccct caatatggct aaattcatta aggatcagtc gctgctgttg ctggaaaagc
     4981 tgaacgagct ggacatggat accccggccg acatgtgcga acggcttcac gaggatgcgg
     5041 aaacactctg gcaggtgatg cgggataagc tcggggaaga ataacagcac cggccatgcc
     5101 ggtgctggcg ttattttttg ccgaacggcc accaccggcg ggcgggtttg tcctccggca
     5161 gttcaggcac cggcacgtta aaatagcggc tgttatattc ccgcatcagc tccgctttca
     5221 gcttctcgtt caccgcgctt tccccgtgcc gctcgctgac cacctcaatc atcgcgttaa
     5281 tctgctccgg tgagcacttc ccggcatcaa ccacctgcac accgttctcg atggcgaacc
     5341 gctccacctt tcgatagagg taatcacaca gcttcattcc tgtgggtttg tcccgttccc
     5401 attgcggatt ttcaccggtg aaaaagtacg tggctttttc gtcgctgttg agccgctcgt
     5461 catacatccc gatggatttc atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa
     5521 catacgcccc ggtctgccag gcgagcacca ccatgtcttc cagatgctcg ctggttggca
     5581 taatgcagcg ctcgccgtct ttgtcttcca cgtcctcaat aaataccatg tacagcgcca
     5641 taatccctca ctccgttgtc ataaatttaa agcacgcgaa cagcctctcc cggtccagct
     5701 ccaccgtttt gcgggcctcg ccggtctgtg gattcacgat ttcatccacc gccagaatcg
     5761 cccgctgtac ccggacaagc gtcggccggt cagggcagat gtaccagatt tcatcgatgc
     5821 cgttcgcccg gcggttgaac agatacccgg acaccacttc ctgataccgc ctgcggctct
     5881 tgacggtgcg ctctacctca aatgccaccg ttctgccgtc cagctccgcc agcgcgtccg
     5941 gcactttcat ccctttcagg gccatacggt gcaggtaccg ccagccggtg gccccccggc
     6001 cctccatgga gaggcgcagg gactgcacgg ccagccggtg cggcagctgc gccgccgaca
     6061 cccttgacgg ctgaaacgga ataacgtccg tcagcggctc gtcctcgtcg aacgagaacg
     6121 ccaccccgtg cggcgtcagc ccccacacgc tgacctgacg gccgtcaacc gggtatttcg
     6181 cccggaccag cagccctttc gccaccagac tgtccaggaa gcggtaaacc ggctgtaccg
     6241 ccgcaatccc cagccgtcgc gcaaccagct ccgccgtgga acacagctct tcccgcagcc
     6301 agcgcagggt cagccgtgtt ttctccgcgt tccgcgcctg ccgcttcgcg tggctgacgt
     6361 tgtactgctc cagtgtcatc agtaaagccc cgcaaaatca tcatccgctt ttggtccgcc
     6421 gccgtcggcc ggaggcaggt actccgccag cgggtccgtt ttcgccagca ccgggaaggt
     6481 tttcagcgaa atttcccggc gctcaacccg cagcgggctg ctgaacgcca gccgcgccac
     6541 gcccagcccg gtcatcaccg cgaacccgtc aggcaggtgt tgcagggtgt tcacgtcaaa
     6601 cagcggccgc gcctccttct ggatgaagct gtcgccgctg atgtgctcca cgttccccgc
     6661 ctcccggctg gcccggcggc gctccacgtc cacccggatc tccccggtct ggcccgccgc
     6721 ccactgcgcg gactcctggc tggccgcgcg atagaaccag cggatcggcg tgttatccag
     6781 taccgtggtt ttcacaaaat cagcgggcag atcctgcccg cactgaccga agtcgcccag
     6841 cgactggtgc gcgagcagca ggttgcagtc ccggctgcgc agcgtcccca gcgcgttcag
     6901 cacgtacttc gacagcagat atttaatctc atcgagcatg atgctcgcgt gcggccacct
     6961 ccggaactca tcccgcgcaa tgatgatttg cgcgcagcgg gcaaacagca tcttctgcac
     7021 ccggatcacc gcctcatcgt ccatcgagcc gatgacgtac aggcagccgc cgccgttgat
     7081 tatccccgcc acatcgatac cgctgtccgt ctgcaggacg ctcaggtcgg ccacgttctc
     7141 cagcttggtg atcagcccct tcacgtcctt tttcagggcc tccggcaacg cgtacgccgc
     7201 cgccagcacc tgctgaatgt ttgccccctg cgggaactgc tccgcaatca gcttcgccgc
     7261 tttctgctcg ctgatgcggt agtggtccgc cacgtcgccg gtctcactca ggttaaaccc
     7321 ggcaaccagc agattcttca gggcgtactg gtcgagatcg cggaacagat tcagctgcgc
     7381 cggtttcccc ggccgcagat ccagcagggt gaagtccggg cagtgcgccc ggaagacgtg
     7441 cggcgcccac gcgtcccctt tggggtcgaa gtacaccaca gcgtccccga aatcgcgtac
     7501 gcagcgaatg agcgcgttgg tggccatcgc gctcttgccg ctcccgggca ggccgaggat
     7561 ctgaatattg gtcttgcgcc aggtatcacg agggaccagc accggctggc cctgctcatc
     7621 cttacccaga aacaccgcct gctgtttccg ggcctgctcc gtggaaaaat cggccagcgc
     7681 gttaaccggt accggctccc cgaaatgcag gtggcggacg tctgtcattt ctccggtccg
     7741 ctggctgctg cgccggctct cctgattcag tttcgcgttc accgcctcgt cagcggccgc
     7801 aaaatgacca tcaagcagca ggaacgccag cagccgcgcc agaaggacgc cggcgacaag
     7861 ccctccggca caccaggcta tcccgaacag gttagccggc tgcagcaggt aataatctgc
     7921 gttcgcatag aggtccgggc gcaggacggt ttctccccag ctctcgataa acacggcgac
     7981 aaccagcccc cccagaaggg ccagaccggc agcgccggtc agccagtacg cgtagttcca
     8041 gacatgtcgc cgtgccctga ccatccgccc gggtgaatcc agcgaggcca gcaccgaaaa
     8101 tggcacagtc cggttattat cgcgcttata gcgatcggca tactcccgcc gcgccttatg
     8161 gctcagcccc acaaaacgca ttcggccggc ccagagttca gccagaccga aaaacaggcc
     8221 gataaccaga aaaaaaccgg ccagactgta atactcccgg tacagccagc tgacccacgg
     8281 gtaaatctgc cgggccagct ccgggatatg ccactgccag gcaagcgaat ccgtattaaa
     8341 catgcaatct ctctccttca gacgacgggt ttaccggcgt gatgcgcagc ccccgctgca
     8401 caagccgcgg acatgcccca aggcatggcg gcggcggtca cgtccgtggt gattgcgttc
     8461 agctttcacc gcggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag
     8521 gaacaacgag cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc
     8581 cggtgttccg gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag
     8641 gagcagcttt gctgcttcgt caggtgcgtc cagcaccgtg gatttttgtg ggtggtcagg
     8701 cgtgagcctg acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacagagc
     8761 cagaaagagg ggaaaagacc gtgcgtcggt ggcgtccagc cagcgtaagc aaggtctttt
     8821 cccctgaagt cagccacgcc ccagcgggga tgacttgcat ttctccgcaa ggagaaaaaa
     8881 gcaacagaga taggttaacg taaaatgttc agcgaaaaac cgacatggtt cacctatcct
     8941 gataattgat cgtcagggaa tagaaagact taatcagggg acaatatccc acatcagcgc
     9001 gtccggtgac cgtcttgcag gtgccgggga aattgcgcct cctcgctcac tcggtcgcta
     9061 cgctccgggc gtgagactgc ggcgggcgct gcggacacat acaaagttac ccacagattc
     9121 cgtggataag caggggacta acatgtgagg caaaacggca gggccgcgcc ggtggcgttt
     9181 ttccataggc tccgccctcc tgccagagtt cacataaaca gacgcttttt cggtgcatct
     9241 gtgggagccg tgaggctcaa ccatgaatct gacagtacgg gcgaaacccg acaggactta
     9301 aagatcccca ccgtttccgg taggtcgctc cctcttgcgc tctcctgttc cgaccctgcc
     9361 gtttaccgga tacctgtccg ccgttctccc ttgcgggaag tgtggcgctt tctcatagct
     9421 cacacactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     9481 actccccgtt cagcccgact gctgcgcctt atccggtaac tgttcacttg agtccaaccc
     9541 gggaaagcac gcaaaacgcc actggcagca gccattggta actgggagtt cgcagaggat
     9601 ttgtttagct aaacacgcgg ttgctcttga agtgtgcgcc aaagtccggc tacactggaa
     9661 ggacagattt ggttgctgtg ctctgcgaaa gccagttacc acggttaagc agttccccaa
     9721 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     9781 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga
     9841 tccccgtcag ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat
     9901 aatttgcctg acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag
     9961 tctttacctt tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca
    10021 ccggaagtca gtatttcaaa tcatccttga taattttttg
//
