LOCUS       NZ_LC505603             3549 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain A1-1 plasmid pCol440I-A1-1, complete
            sequence.
ACCESSION   NZ_LC505603
VERSION     NZ_LC505603.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hayashi,W., Tanaka,H., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     Colistin-resistant Klebsiella spp. isolates from influent municipal
            wastewater in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Hayashi,W., Tanaka,H., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2019) Contact:Wataru Hayashi Shinshu University
            Graduate School of Medicine, Science and Technology, Medical
            Sciences, Health and Medical Science Unit; 3-1-1 Asahi, Matsumoto,
            Nagano 390-8621, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: A5-miSeq pipeline v. 20160825
            Sequencing Technology             :: Illumina HiSeq 4000
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 03:05:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LC505603.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="A1-1"
                     /isolation_source="influent municipal wastewater"
                     /db_xref="taxon:573"
                     /plasmid="pCol440I-A1-1"
                     /geo_loc_name="Japan:Nagano, Matsumoto"
                     /collection_date="2017"
     gene            complement(1..156)
                     /locus_tag="H5A76_RS00005"
     CDS             complement(1..156)
                     /locus_tag="H5A76_RS00005"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(264..521)
                     /locus_tag="H5A76_RS00010"
     CDS             complement(264..521)
                     /locus_tag="H5A76_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_025714833.1"
                     /translation="MAGNKISDFTEEEFTAFVSKIIAADFPTDKENDDAIYSFAQLTEH
                     PAGWNLIFKPKVGEDSSAKGIVKTVKEWRAANGKPGFKKA"
                     /name="bacteriocin immunity protein"
     gene            complement(523..>1125)
                     /locus_tag="H5A76_RS00015"
                     /pseudo=""
     CDS             complement(523..>1125)
                     /locus_tag="H5A76_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178051.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="klebicin B-related nuclease bacteriocin"
                     /name="klebicin B-related nuclease bacteriocin"
     gene            <1124..1465
                     /locus_tag="H5A76_RS00020"
                     /pseudo=""
     CDS             <1124..1465
                     /locus_tag="H5A76_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178052.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(2213..2635)
                     /locus_tag="H5A76_RS00025"
     CDS             complement(2213..2635)
                     /locus_tag="H5A76_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_025714834.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTSINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKREPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2635..2826)
                     /locus_tag="H5A76_RS00030"
     CDS             complement(2635..2826)
                     /locus_tag="H5A76_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3231..3392
                     /locus_tag="H5A76_RS00035"
     CDS             3231..3392
                     /locus_tag="H5A76_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049165871.1"
                     /translation="MHEPPVQSDCCVLSGNYHLESNPERHDKAPLAAATGNGFRGVGLE
                     VMRLLRLN"
                     /name="hypothetical protein"
     Typing          2875..3341
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.979"
                     /coverage="92.0"
                     /evalue="2.73e-170"
ORIGIN
        1 ttacggattg ccgttcgtac cgctatcaag cgtgactccg gtcaccgtcg atgaaggtga
       61 aacagcccca cctccggtgt ctcgaacatt attgacctga catgctgcca gaaccacaga
      121 aacggtaaca acccctagga aaagtaattt tttcatatat caccttttat ttttattatt
      181 ctaggcaaat ggatatctag taattattct tcaaatccag agttacaaat gagcgggctc
      241 ataacttgaa cccgcaaatt caatcacgct tttttaaaac ctggcttacc gttagcagct
      301 cgccattcct ttacggtttt aacaattcct ttcgccgaag aatcttcgcc taccttaggc
      361 ttgaagatga ggttccaacc agcaggatgc tctgttagtt gggcgaacga ataaatagca
      421 tcatcgttct ctttgtcagt tggaaaatca gcagcgatta ttttactaac aaaagcagta
      481 aattcttctt ccgtaaaatc tgaaatttta ttaccagcca ttttattttc ctttatgtgt
      541 ctcaatatga tttttaggtg ttagcgcctt aatgttatcg acatccataa cagcccctcc
      601 ttgagatatt tctttttcgt ggtgtaattc aattttaaca cgtttcccta ctgattctaa
      661 aaagtccgca gtaggtgccc ttccagcttt catctgatta atatcagctt cactaaattg
      721 cttgctcagt gcagaatcgt cagcaaccgc tttccagaac gcccgacgga aagaatcaaa
      781 actaccgaaa gtcttcccgc gtaatttatc cgcaatctgg gaaggaacag gagcaccatc
      841 cccggtactg gcccccccca tccagttatt gccaacgttc tgccctttgc cgctcactgt
      901 accgggcatg ttacgagggc tacggtacat cacatacagc ggttttaggc cggattccgg
      961 aggaaacacc agaataatat cgtcaaaatc gaggtcatcc gctaccggcg tcacggtttt
     1021 atctgtagcc tgtggcgccg aaatctgatc accggtatgc agaatacgct ccggtagtgg
     1081 taccggcccg cttagcgtca gtggtccgtt tgcaccaggt gcgtcaagtg cactagattt
     1141 caccaggatg gcgcttaatc aggaaaaata cctcgatgac tttacatgga aagaactgaa
     1201 cgacgttatc aacttcatac gcaaagcaga aggtagtgca tctgaccaca gctatgcact
     1261 ccagttactg gagattaatt tcaaggcaaa cccactggat ctcatctatc accctgactg
     1321 ctggttcaat gatgaagcat tgtttcatgc ccggctgact accgaagaaa ttggtggcta
     1381 cttaatgaaa aaatcaggtc gatggctcaa cgatgcaccg gatatccaac tggtgtatgc
     1441 catcccccag gatgtttatg attaaccaag tcaccattta ccataatccc ttaattgtac
     1501 gcaccgctaa aacggcctca ggacgtttct gacgggaaac cggtaaaaca ggcaacaaac
     1561 cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat gagtatgcgc
     1621 gcaagtgcat agcgcggaaa ttcacgccat tcagccaggg taagtcctgt ttccggcgtt
     1681 cttctgatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg aggcgttgcc
     1741 ggcgcagccg gaaaaccggt tttcagcacg ttcaggaatc gggttcgtcg acggccggca
     1801 atcacgttat ggcaagtcgc cggacgactc accacacgat tcaccggcga ttcgaatcgt
     1861 gaattgaatc aagagtcacc atgaacaaaa aacgcccaaa ttgatgaatt gaatcaagag
     1921 tcgattcatc ataacaacat gataataaag cgtgattcac atatgaatcg tgggcaattc
     1981 gtaaacgatt cgccatagag tcgtccacga cttggatctg cactttgtaa cacatcactg
     2041 cacattaatt tttcattaac caattgtttt aaataaggaa tctgcaaaat tttaattttg
     2101 cgtagtgtgt gggtattttt agtcgtgagg agcgttcaga gatctggcga tttttgggaa
     2161 ggttcaggaa ctccctggga tggccggaga ggaaaactca agcatggcag gatcaggatt
     2221 ttttccagaa tttccaccac ggggaatggg gtggtgaaga aggctcgcgc ttgtgctcca
     2281 gaagctgcat agcctgacga agggaatcaa tatgctcatc tcgagcctta acagcccctt
     2341 taagaccatc gttttcagct tcaagtagag cgattctatg ttgtaaatca tgtaaaccaa
     2401 cgctgttaat acttgtagat tctgcgtcac tttttacatc atattttaca gtatcgatga
     2461 caccatcaag agaaccaaaa actctaataa gctctgaagt atcaataact ttaatagcct
     2521 tcccgtgctt tacaacagag gtgtaactta acttcccact gttaatcatc ttgtaaattg
     2581 tgggacggct aaccttaaca agccgtgccg cttcactaat agacactctg gccatcaatt
     2641 gccatcctgt aattcatcca gtctcgagga cagtgtacgg aaaagatgct ccgcatcatc
     2701 gtggagtttc tcacaaaggt cggcctcaac atcgagatca agctcgttaa gcttctccag
     2761 caggagcaga gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt
     2821 cgtcataaag ggcctcagag cgtcagtgct caatgtcaga cagatctctt ttccgcttcc
     2881 tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg cgagcggtcg caacgcgcac
     2941 ataacgacat agagcagaca gctcggagag ggagaaggca gcacagcggc cgtttttcca
     3001 taggctccgc ccccctgaca agcatcacag aatttgacgc tcaaatcagt ggtggcgaaa
     3061 cccgacagga ctataaagat accaggcgtt tccccctgat ggctccctcg tgcgctctcc
     3121 tgttcctgcc attcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     3181 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     3241 cccccgttca gtccgactgc tgcgtcttat ccggtaacta tcatcttgag tccaacccgg
     3301 aaagacacga caaagcgcca ctggcagcag ccactggtaa cggatttaga ggagttggtc
     3361 ttgaagtcat gcgcctgtta aggctaaact gaaagaacaa tttttggtga ctgcgctcct
     3421 ctaagccagt taccttggtt caatcacctg caagtgtttg aaccttcgga aaaaccgccc
     3481 tgcaaggcgg ttttttcgtt tacagagcaa gagattacga cgatccaaat gggatctcaa
     3541 gaagatcct
//
