LOCUS       NZ_LC505458             9295 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain A1-1 plasmid pColRNAI-A1-1, complete
            sequence.
ACCESSION   NZ_LC505458
VERSION     NZ_LC505458.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Hayashi,W., Tanaka,H., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     Colistin-resistant Klebsiella spp. isolates from influent municipal
            wastewater in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9295)
  AUTHORS   Hayashi,W., Tanaka,H., Iimura,M., Nagano,Y. and Nagano,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-OCT-2019) Contact:Wataru Hayashi Shinshu University
            Graduate School of Medicine, Science and Technology, Medical
            Sciences, Health and Medical Science Unit; 3-1-1 Asahi, Matsumoto,
            Nagano 390-8621, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: A5-miSeq pipeline v. 20160825
            Sequencing Technology        :: Illumina HiSeq 4000
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/09/2024 03:10:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LC505458.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="A1-1"
                     /isolation_source="influent municipal wastewater"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI-A1-1"
                     /geo_loc_name="Japan:Nagano, Matsumoto"
                     /collection_date="2017"
     gene            1..258
                     /locus_tag="H5B12_RS00005"
     CDS             1..258
                     /locus_tag="H5B12_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            343..492
                     /locus_tag="H5B12_RS00010"
     CDS             343..492
                     /locus_tag="H5B12_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1973..3937
                     /locus_tag="H5B12_RS00015"
     CDS             1973..3937
                     /locus_tag="H5B12_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3937..4668
                     /locus_tag="H5B12_RS00020"
     CDS             3937..4668
                     /locus_tag="H5B12_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            4675..5205
                     /locus_tag="H5B12_RS00025"
     CDS             4675..5205
                     /locus_tag="H5B12_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5233..5412)
                     /locus_tag="H5B12_RS00030"
     CDS             complement(5233..5412)
                     /locus_tag="H5B12_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5481..5858
                     /locus_tag="H5B12_RS00035"
     CDS             5481..5858
                     /locus_tag="H5B12_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5920..6219)
                     /locus_tag="H5B12_RS00040"
     CDS             complement(5920..6219)
                     /locus_tag="H5B12_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6209..6472)
                     /locus_tag="H5B12_RS00045"
     CDS             complement(6209..6472)
                     /locus_tag="H5B12_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6778..7197)
                     /locus_tag="H5B12_RS00050"
     CDS             complement(6778..7197)
                     /locus_tag="H5B12_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7601..9286
                     /locus_tag="H5B12_RS00055"
     CDS             7601..9286
                     /locus_tag="H5B12_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2601..9295
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(596..1174)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3938..4665
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atggggctta aattacatat tcattggttt gataagaaaa ccgaagagtt taaaggtggt
       61 gaatactcaa aagacttcgg tgatgatggt tctgtcattg aaagtctggg gatgccttta
      121 aaggataata ttaataatgg ttggtttgat gttgaaaaat catgggtttc gatattacag
      181 ccacacttta aaaatgtaat cgatattagt aaatttgatt actttgtatc ctttgtttac
      241 cgggatggta actggtaagc aaaagttatc aaggatgatt tgaaatacag acttccggtg
      301 ttcagggatg aataccgggg tttttaaata cagggaaggt atatgaaaaa ggtaaaggtt
      361 gtttttcttt ttatattgat tttttcaggc tttcttctgg tcgcatgtca ggcaaactat
      421 atccgggatg ttcagggtgg aacggtggca ccatcgtcct cctctgaact gacggggatc
      481 gcggttcagt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
      541 tttgccctgt aaacgaaaaa accacctggg gaggtggttt gatcgaaggt taagtcagtt
      601 ggggaactgc ttaaccgtgg taactggctt tcgcagagca cagcaaccaa atctgtcctt
      661 ccagtgtagc cggactttgg cgcacacttc aagagcaacc gcgtgtttag ctaaacaaat
      721 cctctgcgaa ctcccagtta ccaatggctg ctgccagtgg cgttttgcgt gcttttccgg
      781 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggggttc
      841 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agagtgtgag
      901 ctatgagaaa gcgccacact tcccgcaagg gagaaaggcg gaacaggtat ccgggaaacg
      961 gcagggttgg aacaggagag cgcaagaggg agcgattcat cggaaacggt gatgatcttt
     1021 aagtcctgtc gggtttcgcc actcctgact gattcatggt tgagccaccg gctcccacag
     1081 atgcaccgaa aaagcgtctg tttatgtgaa ctcagtcagg agggcggagc ctatggaaaa
     1141 acgccacctg cgtggccttg ccgttttgcc tcacatgtta gtcccctgct tattcacgga
     1201 atctgtgggt aactttgcat gtgtccgcag cgcccgccgc agtctcacgg ccggagcgta
     1261 gcgaccgagt gagcgaggag gcgcaatttc cccggcacct acaagacgga caccggacgc
     1321 gctgatgtgg catattgtcc cctgattaag tctttatatt ccctgataat aaattaccag
     1381 gataggtgaa tcatatcggt ttttcgctga acattttacg ttaacctatc tctgttgctt
     1441 ttttctcctt acggagaaat acaggtcatc cccgcgaggg cgtggctgac ctcaggggga
     1501 tatcccctgc ttacgctggc tggacgccac cgacgcacgg tctctccccc tctttctggc
     1561 tccgtccggc tggacgccgg ccacgccaga aattcacccg gcccccgtca ggctcacgcc
     1621 tgaccaccca caaaaatcca cggtgctgga cgcacctggc gaagcagcaa agctgctcct
     1681 gattttgcgg gtggtcagcc gctcgctatg gcgaactctg acgggggccg gaacaccgga
     1741 aaagaccgca ctggggtgcc gccttttcct ctcacacgct ggggcgtgct cgttgttcct
     1801 ccggggctgc gccttttccg catggggcgg aaagaggctg gcctccgtgg tgaaagctga
     1861 acgcaatcac cacggacgtg accgccgccg ccatgccttg gggcatgtcc gcggcttgtg
     1921 cagcgggggc tgcacatcac gccggcaaac catcgtctga aggagagaga acatgtttaa
     1981 tacggattcg cttgcctgga agtggcacat tccggagctg gcccggcaga tttatccgtg
     2041 ggtcagctgg ctgtaccagg agtattacgg tctggccggg gctttcctag ttatcggcct
     2101 gtttttcggt ctggctgaac tttgggccgg acggatgcgt tttgtggggc tgagtcataa
     2161 ggagcgacgg gagtatgccg atcgctataa gcgcgataat aaacggccag cgccattttc
     2221 ggtgctggcc tcgctggatt cgcccgggcg gatggtcagg gcacgtcgac atgtctggaa
     2281 ctacgcgtac tggctgaccg gcgcggccgg tctggccctt ctgggggggc tggttgtcgc
     2341 cgtgtttatc gagagctggg gagaaaccgt cctgcgtccg gacctctacg caaacgcaga
     2401 ttattacctg ctgcagccgg ctaacctgtt cgggattgcc tggtgcgcgg gcgggctggc
     2461 tgtcgggatt gtgttggcgc ggctgctggc gttcctgctg cttgacggtc attttgcggc
     2521 tgctgacgag gcggtgaacg cgaaactgaa tcaggagagc cggcgcagca gccagcggac
     2581 aggagagatg accgacgtgc gccacctgca tttcggggag ccggtaccgg ttaatgcgct
     2641 ggccgatttt tcctcggagc aggcccggca ggagcaggcg gtgtttctgg gtaaggatga
     2701 gcagggcaag ccggtgctgg ttccgcgtga tacctggcgc aagaccaaca ttcagatcct
     2761 cggcctgccc gggagcggca agagcgtgat ggccaccaac gcgctcattc gctgcgtacg
     2821 cgatttcggg gacgcggtgg tgtacttcga ccccaaaggg gacgcatggg cgccgcacgt
     2881 cttccgggcg cactgtccgg acttcaccct gctggatctg cggccgggga aaccggcgca
     2941 gctgaatctg ttccgcgatc tcgaccagta cgcgctgaaa aacctgctga ttgccgggct
     3001 taacctgggc gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc
     3061 gaagctgatt gcggagcagt ttccgcaggg ggcaaacatt cagcaggtgc tggcggcggc
     3121 gtacgcgctg ccggaggccc tgaaaaagga cgtgaagggg ttgatcacta ggctggaaaa
     3181 cgtcgctgac ctgagcgtcc tgcagacgga cagtggcatc gatgtggcgg ggattatcaa
     3241 cggcggcggc tgcctgtacg ttatcggctc gatggacgac gaggcggtga tccgtgtgca
     3301 aaaaatgctg tttgcccgct gcgcgcaaat catcattgcg cgggacgagt tccgggagtg
     3361 gccacacgtc agcgtgatgc tcgatgaaat taaatatctg ctgtcgaagt atgtactgaa
     3421 cgcactgggg acgctgcgca gccgggactg caatctgttg ctcgcgcacc agtcgctggg
     3481 cgatttcggg cagtgcgggc aggatctgcc cgctgaattt gtgaaaacca cggtactgga
     3541 taacacgccg atccgctggt tttaccgggc ggccagcctg gagtccgcgc agtgggcggc
     3601 ggggcagacc ggggaaatca gggtggacgt ggagcgccgc cgtgccagcc gggaggcggg
     3661 gaacgtggag catatcagcg gggacagctt catccagaag gaggcgcggc cgctgtttga
     3721 cgtgaacacc ctgcaacacc tgccggacgg gttcgcggtc atgaccgggc tgggtgtggc
     3781 ccggatggcg ttcagcagtc cgctgcgggt tgagcgccgg gaaatcccgt tgcagcgttt
     3841 cccggtgctg gcgaaaaccg atcccctggc ggagttccgg cccccggccg acggcggcgg
     3901 gccgaaagcg gatgatgatt ttgcggggat ttactgatgg cgctggagct gtacaacgtc
     3961 agccacgcga aacgacaggc gcggaacgcg gagaaaaccc ggctgaccct gcgctggttg
     4021 cgggaagagc tgtgttccac ggcggagctg gttgcgcggc ggctggggat tgcggcggtg
     4081 cagccggtct accgcttcct cgacagcctg gtggcgaaag ggctgctggt ccggaccaaa
     4141 tatccggtgg acggccgtca ggtcagcgtg tgggggctga cgccgcatgg cgtggcgttc
     4201 tcgttcgacg aggacgaacc gctggcggat gttattccgt ttcagccgtc aagggtgtcg
     4261 gcagcacatc tgccgcaccg gctggccgtg caatccctgc gccttgatat ggaagccaga
     4321 ggggccaccg gctggcgcat tctgcaccgt ctgagtctta aaggcatgaa agtgccggac
     4381 gccctggcgg agctggacgg taaaacggtg gcgtttgagg tggagcgcac cgtcaagagc
     4441 cgtcgccggt atcaggaagt ggtgtccggg tatctgttca accgcaaagc taacggcatc
     4501 gatgaaatct ggtacatctg cccggaccgg gcgacgcagg tcagggtgca gcgggcaatc
     4561 ctgtcggtgg atgaaatcgt gaacccacag accggcgagg cccgcaaaac ggcagagctg
     4621 gatcgtgaac ggttgtttgc gtgctttaaa tttatgacaa cggagtgagg gattatggcg
     4681 ctgtacatgg tatttattga agacgcggag gaccggaacg gcgagcgctg catcatgccg
     4741 accagcgagc atctggaaga tatggtggtg ctcgcctggc agaccggggc gtatgttttc
     4801 tgttttgtcg accgggccca gcatctggcg atgatgaaat ccatcgggat gtatgacgag
     4861 cggctcaaca gcgacgaaaa agccacgtac tttttcactg gtaaaaatcc gcaatgggaa
     4921 cggaacaaac ccacaggaat gaagctgtgt gattacctct atcgcaaggt ggagcggttc
     4981 gccatcgaga acggtgtgca ggtggttgat gccgggaagt gctcaccgga gcagattaac
     5041 gcgatgattg acgtggtgag tgagcggcac ggggaaagcg cggtgaacga gaagctgaaa
     5101 gcggagctga tgcgggaata taacagccgc tattttaacg tgccggtacc tgaactgccg
     5161 gaggacaaac ctgcccgccg gtggtggccg ttcggcaaaa aataaatgcc agcaccggcc
     5221 atgccggtgc tgttattcct ccccgagctt atcccgcatc acctgccaga gtgtttccgc
     5281 atcctcgtgt agccgctcgc acatgtcggc cggggtatcc atatccagct cattcagttt
     5341 atcgagcaat agcagcgact gctccttaat aaatttagcc atattgaggg ctgttttttc
     5401 ctgttttgtc atgattcttt ccttccgggc atggtgtgcg cctgtcaggt atactgtctc
     5461 aaaaatagcg gggtgagaca atggcgtggg tgacggtgag acaggcgacg gaactgacgg
     5521 gtaaagcccg aagttccctg tatcgggata tggcgaaagg ccgcgtatca taccgtacgg
     5581 aagctgatgg cggccgggtg gtggatacca gcgagcttat ccgggtgtac ggtgagttaa
     5641 gacaggatga gactcacgag cgggacgagt tgagactgtc gggtgagact gaaaaaatgg
     5701 cctccgacgc gttgatcgcg gagataaaag cactcaggga ggaggtggcc gggctacggc
     5761 aggaaatgca gtccatgaga ctgctggaac acaaaccgga gacagcagag accccggccg
     5821 gaacaccgcg ccggtggtgg ccgtggggaa aacgctgacg cgctgtgctt gtccaaccgg
     5881 cgacggccac gcaatgaatt gcgtccccgt caccggttat tagagaacaa tatggggatt
     5941 ctggcgctgg ccgaccacgg ccagaacccg gacttttctg gtctggtcat caacatcata
     6001 gaggacatgc tgtcccatca gactgatccg gcgaacgtcc tcgccatagc gtgagtccac
     6061 ttcatagagt cgcgggaagt cggccagttt gtccgccgca gcgagaaccc ggtcggtaac
     6121 gttctgcgca tcccacaggg atgaattttc cgcgatatag tcaaacagcg tcgttacatc
     6181 ctcgatggct ccctgcttcc attccacgtc atacggcatc gcgcagttcc tgataacggc
     6241 catgcaggcg ctggcggagt tgttccgtgg tcatacccgg acgcgtgtca gcacgggcgg
     6301 cggtcacttt ggcgcgaacc tgatcttcat actctgcctg ggccagttct tcgttagcca
     6361 gtcgctgcaa cagcgcagct ttgtccgggc caccttccgg cagtatgcgg ggcaggcttt
     6421 cagccagttc ccgaagaaag ttggcgtttt cgtagtgtgt agtgttgctc attgcggagc
     6481 ctcccgggtt aatgcggcaa tatgaaggaa gtttagcagg gggcggtctg aaagaatatg
     6541 gtcgtgtcag tcaggctgat gaagcagaaa gtcggtaaaa aagggcttac ggcatccatt
     6601 ttacgtcaaa acatatgcca tgcagttgcg tgcataggca tgcattagga taaaatttat
     6661 cgggcgcgtt tccggcagtt ttccgggggg gttgttgcct gttttatccc gtagccgccg
     6721 gaaacgccct gagcccgtct gagcggtgcg cgtaatgacg cgttatggta aatccactta
     6781 ctgtctgcct tcatagccat cgagataaac agctgaaatg gtgtcagcca gtagccttga
     6841 ttttttcggt gtgaggtctt tgctgcggta atcggtaccc gcaaattgtg ttgtgccttg
     6901 gagtgagttt aaaaattcct gactgtgaaa ctgctgaatg cgttgctgca catcactccg
     6961 tgattttccg gtcgctttat cctttgcgcc catatcccag aactgccgga aaaaaggcac
     7021 catcattcgt gttgtgtcag ctttctgggt ggaaaaattg ggatcaaaac cgtcatctga
     7081 agcatatacg taatgccggg tatgctgttc cacgctgccc tttggagcac aggcagtgag
     7141 agttgctgtc gctgttatca tggcgataag acacagtttg ctgccggaac gtctcatgag
     7201 cgtctcctta cctttaattc ttttttctca ttgagttata tatccgcagg ctatcttatc
     7261 atcagtttct gaccgtgttc agtgaccgaa caacccgaac cagacccccc gtttcttttc
     7321 ctgtcccgga gggggctttc tggtatctca tataatgatt tcagtaaaac atactgaggc
     7381 cggagaacgg cgccgtttgt gctggttttt gttgcatctg gaggggattg cgcatggtgt
     7441 gagtggctgt ttttttgatc attttcacaa aaacgcaaat ttacagactt gacatgaaaa
     7501 acaaggcagc gtaatttttt atactgtata taaaaccagt ggttatatgt acagtatttt
     7561 attttagttt attgttttaa aagtcaaaga ggaaaagtat atgagtggcg gagacggacg
     7621 aggtccgggt aattcaggtc tgggacacaa tggtggtcag gccagtggga atgtgaacgg
     7681 tacatcaggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg
     7741 ctggggtacg acgcatacac ctaacgggga tattcataac tataacccgg gggagtttgg
     7801 taacggcggg agtaaacccg gtggtaatgg cggtaacagc ggcaatcata gcgggagctc
     7861 tggtggcgga cagtcttcgg ccaccgcgat ggccttcggt ctgcctgcct tggccactcc
     7921 gggggctgaa ggactggctt tatccgtttc cggcgatgcg ttgtcggccg ctgttgctga
     7981 tgtgctggct gccctgaaag gtccgtttaa gtttggtctg tgggggattg cgatttacgg
     8041 agtgctgcct tctgagatag caaaagatga tccgaaaatg atgtcaaaaa tcgtgacgtc
     8101 attgccggca gacacggtaa cggagacgcc ggtcagctcc ctgccgctgg accaggcgac
     8161 ggtcagcgtc actaaacgtg tggcggatat tgtgaaggac gagcggcagc atattgcggt
     8221 tgtcaccggc cggccaatga gtgttcctgt ggtggatgcg aaaccgacaa aacgtccggg
     8281 ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac ctaaaggtgt
     8341 tccggccgcg aaagccccgc caaaaggcat tattgctgaa aaaggtgatt cacgtccggc
     8401 tggttttacg gccggtggta actcccgtga agccgttatt cgtttcccga aagagagcgg
     8461 acagaaaccg gtctatgtgt cggtgacgga tgttcttact ccggcacagg taaaacagcg
     8521 tctggaggaa gaaaagcgtc gccagcaggc atgggacgct gctcacccgg aagaggggct
     8581 gaaaagagag tatgataaag cgaaagctga gctggatgct gaagataaaa atattgcgac
     8641 cttaaacagt cgcattgcat cgacagagaa ggcgatcccc ggtgcaaggg ctgctgttca
     8701 ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa
     8761 cccgcctcat gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat
     8821 tcagaatcag aatgagaaat taaaagctgc ccagacatct ttaaacgaaa tgaatgaatc
     8881 cttatccagg gataaggctg cgctttccgg ggcgatggag agccggaaac aaaaggagaa
     8941 aaaagcgaag gatgcagaaa ataaattaaa tgaggaaaag aaaaaacctc gtaagggaac
     9001 taaagattac ggccatgatt attttcctga tcccaagact gaagatatta aggggttggg
     9061 agagttgaaa gagggtaaac ctaaaacccc taaacaaggt ggtggtggta agcgggcccg
     9121 atggtatggt gataaaaagc gtaaaattta tgaatgggat tcccagcacg gtgagcttga
     9181 agggtaccgc gccagtgatg gcgaacacct cggggcattc gatccaaaaa cgggtaagca
     9241 ggttaaaggg ccggatccaa aacgaaacat taaaaaatat ctttaagagg taaat
//
