LOCUS       NZ_LC438465             6646 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain SN38 plasmid pSN38_s1, complete sequence.
ACCESSION   NZ_LC438465
VERSION     NZ_LC438465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMD00080513
            Sequence Read Archive: DRR092875
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Matsumura,Y.
  TITLE     Molecular epidemiology of Escherichia coli sequence type 131
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Matsumura,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-DEC-2018) Contact:Yasufumi Matsumura Kyoto University
            Graduate School of Medicine, Clinical Laboratory Medicine; 54
            Shogoin-kawaharacho, Sakyo-ku, Kyoto, Kyoto 606-8507, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.6
            Sequencing Technology             :: MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009974905.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 02:01:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LC438465.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SN38"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pSN38_s1"
                     /geo_loc_name="Japan:Shiga"
                     /collection_date="2010"
     gene            complement(join(5329..6646,1..251))
                     /gene="cea"
                     /locus_tag="CB779_RS00005"
     CDS             complement(join(5329..6646,1..251))
                     /gene="cea"
                     /locus_tag="CB779_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            511..948
                     /locus_tag="CB779_RS00010"
     CDS             511..948
                     /locus_tag="CB779_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1029..1448
                     /locus_tag="CB779_RS00015"
     CDS             1029..1448
                     /locus_tag="CB779_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1765..1998)
                     /locus_tag="CB779_RS00020"
     CDS             complement(1765..1998)
                     /locus_tag="CB779_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2005..2523)
                     /locus_tag="CB779_RS00025"
     CDS             complement(2005..2523)
                     /locus_tag="CB779_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2561..3259)
                     /locus_tag="CB779_RS00060"
                     /pseudo=""
     CDS             complement(<2561..3259)
                     /locus_tag="CB779_RS00060"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3201..3524)
                     /locus_tag="CB779_RS00035"
     CDS             complement(3201..3524)
                     /locus_tag="CB779_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3588..3779
                     /locus_tag="CB779_RS00040"
     CDS             3588..3779
                     /locus_tag="CB779_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4449..4607
                     /locus_tag="CB779_RS00065"
     CDS             4449..4607
                     /locus_tag="CB779_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4806..4943)
                     /locus_tag="CB779_RS00045"
     CDS             complement(4806..4943)
                     /locus_tag="CB779_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            5105..5332
                     /locus_tag="CB779_RS00050"
     CDS             5105..5332
                     /locus_tag="CB779_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(3865..3952)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3963..4521
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1662..3211)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttgctttcg cctgtgcttc cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt
       61 ttctttaatt gagcagtaga ccatttagca gttgcatgaa tagctgcaga actttcactt
      121 ttactgcctc cttttccacc tccgccgcca gagccactcc cgtcaggagt accattcaaa
      181 agagtaataa ttacctgtcc cttatcatca taaggaacac catctttata gtacgctacc
      241 gcggtttcca ttataaaatc ctctttgact tttaaaacaa taagttaaaa ataaatactg
      301 tacatataac cactggtttt atatacagca taaaagctac gccgctgcat tttccctgtc
      361 aagactgtgg acttccattt ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg
      421 ctattctcgc ccgatgccac aaaaaccagc acaaacatta ccgttctcag acctcattat
      481 gttttactga aactatgaga tgagacatct atgggacact gtcactttat ggcatggcac
      541 acactccggg acgcactaaa aatgacaggc agatcgcgtt cacagtttta ccgtgatatg
      601 cgcgcaggcc ttgtcagtta ccgtaccggc agggacggac gacgggagtt tgaaaccagt
      661 gaactgatcc gggcatacgg cgaattaaag cagaatgaga caccagaaag gcacagtgag
      721 ggacatgcag aaaatccaca tgatcagcag acagaacgca ttctccggga actgaatgag
      781 ctgaaacaat gcctgacgct gatgcttgag gataaacagg cacaggatat ggatcgcaga
      841 cgccaggaag cagaacggga acagctacaa aatgagatag cccagctcag gcaggcactg
      901 gaactggaaa agaaacgggg attctggtcc aggttgttcg gtcgctgaac gctgtcagag
      961 actgatgata aaatagtctt cggataataa ctcaccgaga ataaatactt taaggtaggg
     1021 agacactcat gagacgtacc ggaaacaaac tttgtcttat cgccatgata acagcaacag
     1081 tagctctcac agcctgtacc ccaaagggca gcgtggaaca acatacccgg cattacgtat
     1141 atgcttctga tgacggtttt gatcccaact tttccaccca aaaagccgac acaacacgaa
     1201 tgatggtgcc tttttttcgg cagttctggg atatgggagc taaagataaa gcgacaggaa
     1261 aatcacggag tgatgtgcaa caacgcattc agcagtttca cagccaggaa tttttaaact
     1321 cactccgggg cacaactcaa tttgcgggta ctgattaccg cagcaaagac cttaccccga
     1381 aaaaatccag gctgctggct gacacgattt ctgcggttta tctcgatggc tacgagggca
     1441 gacagtaagt ggatttacca taatccctta ttgtacgcac cgctaaaacg cgttcagcgc
     1501 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     1561 gcctgataaa ttttaaccgt atgaatacct atgcaaccag agggtacagg ccacattacc
     1621 cccacttaat ccactgaagc tgccattttt catggtttca ccatcccagc gaagggccat
     1681 ccagcgtgcg ttcctgtatt tccggctgac gctcccgttc cagggataac acatgttcgc
     1741 gctcctgtat cagccgttcc tctcttatct ccagttctcg ctgtataact ggctcaagcg
     1801 ttctgtctgc tcgctcaagt gctgcacctg ctgactcaac tgcatgaccc gctcgttcag
     1861 catcgcgttg tcccgttgcg taagcgaaaa catcttctgc aattccacga aggcgctctc
     1921 ccattcgctc agccgctgca tatagtcctg ttgcagctgc tctaaggcgt tcagcaaatg
     1981 tgtttccagc tctgtcactc tgtgtcactc cttcagatgt acccactctt tcccctgaaa
     2041 gggaatcacc tccgctgatt tcccgtacgg aaggacaagg aatttcctgt tcccgtcctg
     2101 cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct
     2161 gatttcttcc aggttcgcct gtatcctccc tccaagatac cagagcgtcc cgccactcgc
     2221 ggtaaacagg agaaagacta tccccagtaa catcatgccc gtattccctg ccagctttaa
     2281 cacgtccctc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt tttccagcga
     2341 ttcctctgtc gaggctgtga acagggctat agcgtctctg attttcgtct cgtttgatgt
     2401 cacagcctcg cttacagatt cgccgagcct cctgaactcg ttgttcagca ttttctctgt
     2461 agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgca aaagattgct
     2521 cataaaatgc tcctttcagc ctgatattct tcccgccgtt cggatctgca atgctgatac
     2581 tgcttcgcgt caccctgacc acttccagcc ccgcctcagt gagcgcctga atcacatcct
     2641 gacggccttt tatctctccg gcatggtaaa gtgcatctat acctcgcgtg acgccctcag
     2701 caagcgcctg tttcgtttca ggcaggttat cagggagtgt cagcgtcctg cggttctccg
     2761 gggcgttcgg gtcatgcagc ccgtaatggt gatttaccag cgtctgccag gcatcaattc
     2821 taggcctgtc tgcgcggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt
     2881 tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc
     2941 tgtactgatt tttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacttttt
     3001 cacgtcctcc cggcggcagc tccttctccg cgaacgacag aacaccggac gtgtatttct
     3061 tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg
     3121 ttgcgccttc gcggttacgc tccctcccca gcaggtaatc aaccggacca ctgccaccac
     3181 cttttcccct ggcatgaaat ttaactatca tcccgcgccc cctgttccct gacagccaga
     3241 cgcagccggc gcagctcatc cccgatggcc atcagtgcgg ccaccacctg aacccggtca
     3301 ccggaagacc actgcccgct gttcacctta cgggctgtct gattcaggtt atttccgatg
     3361 gcggccagct gacgcagtaa cggcggtgcc agtgtcggca gttttccgga acgggcaacc
     3421 ggctccccca ggcagacccg ccgcatccat accgccagtt gtttaccctc acagcgttca
     3481 agtaaccggg catgttcatc atcagtaacc cgtattgtga gcatcctctc gcgtttcatc
     3541 ggtatcatta ccccatgaac agaaatcccc cttacacgga ggcatcagtg actaaacagg
     3601 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg ctgctggaga
     3661 agctcaacga actggacgca gatgaacagg ccgatatttg tgaatcgctt cacgaccacg
     3721 ccgatgagct ttaccgcagc tgcctcgcac gtttcgggga tgacggtgaa aacctctgac
     3781 acatgcagct cccggagacg gtcacagctt gtctgtgagc ggatgccggg agctgacaag
     3841 cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccctg acccagtcac
     3901 gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcgga ttgtatgaaa
     3961 agtacgccat gccgggtgtg aaatgccgca cagatgcgta aggagaaaat gcacgtccag
     4021 gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     4081 ggtactgact cacacaaaaa cggtaacaca gttatccaca gaatcagggg ataaggccgg
     4141 aaagaacatg tgagcaaaag accaggaaca ggaagaaggc cacgtagcag gcgtttttcc
     4201 ataggctccg cccccctgac gagcatcaca aaaatagacg ctcaagtcag aggtggcgaa
     4261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4381 cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc
     4441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     4501 gtcttgagtc caacccggta aggcacgcct taacgccact ggcagcagcc actggtaacc
     4561 ggattagcag agcgatgatg gcacaaacgg tgctacagag ttcttgaagt agtggcccga
     4621 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4681 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgttggta gcggtggttt
     4741 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttaat
     4801 cttttctact gaaccgcgat ccccgtcagt ttagaagagg aggatggtgc gatggtccct
     4861 ccctgaacat cacgtatata gttagcctga catccaacaa ggaggtttat cgcgaatatt
     4921 cccacaaaaa atcttttcct cataactcga tccttataaa atgaaaagaa tatatggcga
     4981 ggtttaattt atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac
     5041 acttatatat atatacctta taacaaaaaa cagcgaaggg tattatttcc ttgtgtcaga
     5101 taagatgcta tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat
     5161 tgaatacata gcttttaact tcataaagaa agattttttc gaaagaagaa aaaacctaaa
     5221 taacgccccc gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct
     5281 agcaatccca tttggattgc taggactttt tatatcaata aagaataatt aaatccctaa
     5341 cacctcattt atagtattaa gtttattctt atcaatatag gagcatagaa ttcctgtaac
     5401 aatagcaata ccccaaatac ctaatgtagt tccagcaagc aagctaaaaa gtaaagcaac
     5461 aacataactc acccctgcat ctgcagcttt cttctctaat gtaagaaata gtggcttcca
     5521 gtcacctgta tccttaattt ttaggatatc agataccaca tcatatccaa aagaaacatg
     5581 ccccgtaatc ttcaagtact tggcaaactg atctaaatgt ttagcccagt catcatactt
     5641 caccgatgcc aacgcattaa aaatagcatc acgatcggct ttgctgaatt tcttatttaa
     5701 aacatccttg tatttttcaa aagcagcgag agcttcattc acattgccga ttttcttacc
     5761 tttagactta tcagcaagtt cctgtgccat tttcgaatat ttttcaccat atttttcagt
     5821 cagcgtttga taaaagctaa ctgttgcatc aacagcatcc ttaatctgtg aattaaggag
     5881 attattctgt gcttttttca aattttcttc agcttcatga acacgagcga taccggcatt
     5941 acgattatta ctgacctgag aaatagcctt ctggatctga gttatatcag catttatccg
     6001 gttaatacgt gtttctgatg ctgttacctg tttttgtttt tcttctctaa tcttaccggc
     6061 cccaacccgt cgtctggttg cttcaaaaaa aggacggttc tgaagcggat cattggctct
     6121 tggtgatagt tttttgacca gctcatccag ttctttatat ttagcggatg cctgagccag
     6181 ttcatttcgt tttccagcga gcgttttcat ttctgcatca cgggcatgga tactggagct
     6241 taaacgagaa ttgagagtct taatctctcc atccattttc accacttcag attgtgcagc
     6301 agaaagtttt ttttgggcga tctcaacagc tttagcttct tcactcaatg cagccagtcg
     6361 tttctcttca gcttcagcca gtttcaactg gcgttctgtt tcagccttct cccgttcaat
     6421 ctctttacgt cgttgttctg cttcctgaaa agccttttct gctgcttccg cttctttacg
     6481 ggctttttct tctgctttcg caaggcgcaa acgctctgct tccgcctgca tagctgcatt
     6541 attagcatga gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac gaagagcctc
     6601 attcacgata tccttcaggc gctgagtcag cgcatccctg tttgcc
//
