LOCUS       NZ_LC086373             2473 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Staphylococcus aureus strain KT1 plasmid pWKT1, complete sequence.
ACCESSION   NZ_LC086373
VERSION     NZ_LC086373.1
DBLINK      BioProject: PRJNA224116
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Yamamoto,T.
  TITLE     Erythromycin/clindamycin resistance plasmid sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Yamamoto,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2015) Contact:Tatsuo Yamamoto International
            Medical Education and Research Center; Fukusumi Building II, 1-86-12
            Higashinakadori, Chuo-ku, Niigata 951-8116, Japan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009974665.1-RS_2024_05_27
            Annotation Date              :: 05/27/2024 02:46:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LC086373.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="KT1"
                     /db_xref="taxon:1280"
                     /plasmid="pWKT1"
                     /note="ST121/agr4 CA-MRSA from pneumonia"
     gene            389..865
                     /locus_tag="HXG58_RS00005"
     CDS             389..865
                     /locus_tag="HXG58_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1560..1619
                     /gene="ermCL"
                     /locus_tag="HXG58_RS00010"
     CDS             1560..1619
                     /gene="ermCL"
                     /locus_tag="HXG58_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            1680..2414
                     /gene="erm(C)"
                     /locus_tag="HXG58_RS00015"
     ARG             1681..2411
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1681..2414
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          390..875
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgattatg tcttttgcgc attcacttct tttctatata aatatgagcg aagcgaataa
       61 gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg ttcagggggt
      121 gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt acgttagata
      181 accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg taaaatctta
      241 atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt tcactcattt
      301 tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga tatagttaga
      361 atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag tctataaagg
      421 ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca agttataccg
      481 taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa gtatgttaga
      541 tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg tccacttaag
      601 taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa caagtctaca
      661 aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa gaaaaactgg
      721 agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac aaaaatacct
      781 cttactcgaa tttgggaact ttgagcaaga ggcaaatgaa aaacaagaaa atgcattatc
      841 tgattattat tctttcaagg actagtataa cataaaatcg tctacaaata gacaaaaaac
      901 ctgcacgctt aatgtagatc aaaagcttaa cgcaaatgaa atagattgac ctcccaataa
      961 caccacgtag ttattgggag gtcaatctat gaaatgcgat taagcttttt ctaattcaca
     1021 taagcgtgca ggtttaaagt acataaaaaa tataatgaaa aaaagcatca ttatactaac
     1081 gttataccaa cattatactc tcattatact aattgcttat tccaatttcc tattggttgg
     1141 aaccaacagg cgttagtgtg ttgttgagtt ggtactttca tgggattaat cccatgaaac
     1201 ccccaaccaa ctcgccaaag ctttggctaa cacacacgcc attccaacca atagttttct
     1261 cggcataaag ccatgctctg acgcttaaat gcactaatgc cttaaaaaaa cattaaagtc
     1321 taacacacta gacttattta cttcgtaatt aagtcgttaa accgtgtgct ctacgaccaa
     1381 aagtataaaa cctttaagaa ctttcttttt tcttgtaaaa aaagaaacta gataaatctc
     1441 tcatatcttt tattcaataa tcgcatcaga ttgcagtata aatttaacga tcactcatca
     1501 tgttcatatt tatcagagct cgtgctataa ttatactaat tttataagga ggaaaaaata
     1561 tgggcatttt tagtattttt gtaatcagca cagttcatta tcaaccaaac aaaaaataag
     1621 tggttataat gaatcgttaa taagcaaaat tcattataac caaattaaag agggttataa
     1681 tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat aatatagata
     1741 aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc ggctcaggaa
     1801 aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc attgaaatag
     1861 accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat ttccaagttt
     1921 taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa atatttggta
     1981 atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat agtatagctg
     2041 atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat acaaaacgct
     2101 cattggcatt atttttaatg gcagaagttg atatttctat attaagtatg gttccaagag
     2161 aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat agaaaaaaat
     2221 caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa tgggttaaca
     2281 aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa catgcaggaa
     2341 ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat agctataaat
     2401 tatttaataa gtaagttaag ggatgcataa actgcatccc ttaacttgtt tttcgtgtac
     2461 ctattttttg tga
//
