LOCUS       NZ_KY770023            33051 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain ICBEC2AM plasmid pICBEC2AM, complete
            sequence.
ACCESSION   NZ_KY770023
VERSION     NZ_KY770023.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227851
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33051)
  AUTHORS   Fernandes,M.R., Sellera,F.P., Esposito,F., Cerdeira,L. and
            Lincopan,N.
  TITLE     Colistin-Resistant mcr-1-Positive Escherichia coli in Public
            Beaches, an Infectious Threat Emerging in Recreational Waters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33051)
  AUTHORS   Fernandes,M.R., Sellera,F.P., Esposito,F., Cerdeira,L. and
            Lincopan,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2017) Microbiology, USP, Av. Prof. Lineu Prestes,
            1374, Sao Paulo, SP 05508-900, Brazil
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: Spades v. 3.7.0
            Assembly Name                     :: ICBEC2AM
            Coverage                          :: 68
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009973915.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:40:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 40
            CDSs (total)                      :: 40
            Genes (coding)                    :: 39
            CDSs (with protein)               :: 39
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KY770023.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ICBEC2AM"
                     /isolation_source="seawater"
                     /db_xref="taxon:562"
                     /plasmid="pICBEC2AM"
                     /geo_loc_name="Brazil"
     gene            50..565
                     /locus_tag="HTM09_RS00005"
                     /old_locus_tag="ICBEC2AM_00001"
     CDS             50..565
                     /locus_tag="HTM09_RS00005"
                     /old_locus_tag="ICBEC2AM_00001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            670..1212
                     /locus_tag="HTM09_RS00010"
                     /old_locus_tag="ICBEC2AM_00002"
     CDS             670..1212
                     /locus_tag="HTM09_RS00010"
                     /old_locus_tag="ICBEC2AM_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            1209..1460
                     /locus_tag="HTM09_RS00015"
                     /old_locus_tag="ICBEC2AM_00003"
     CDS             1209..1460
                     /locus_tag="HTM09_RS00015"
                     /old_locus_tag="ICBEC2AM_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            1447..1674
                     /locus_tag="HTM09_RS00020"
                     /old_locus_tag="ICBEC2AM_00004"
     CDS             1447..1674
                     /locus_tag="HTM09_RS00020"
                     /old_locus_tag="ICBEC2AM_00004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            1970..2188
                     /locus_tag="HTM09_RS00025"
                     /old_locus_tag="ICBEC2AM_00005"
     CDS             1970..2188
                     /locus_tag="HTM09_RS00025"
                     /old_locus_tag="ICBEC2AM_00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            2279..2629
                     /locus_tag="HTM09_RS00030"
                     /old_locus_tag="ICBEC2AM_00006"
     CDS             2279..2629
                     /locus_tag="HTM09_RS00030"
                     /old_locus_tag="ICBEC2AM_00006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            2934..3182
                     /locus_tag="HTM09_RS00035"
                     /old_locus_tag="ICBEC2AM_00008"
     CDS             2934..3182
                     /locus_tag="HTM09_RS00035"
                     /old_locus_tag="ICBEC2AM_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            3179..3526
                     /locus_tag="HTM09_RS00040"
                     /old_locus_tag="ICBEC2AM_00009"
     CDS             3179..3526
                     /locus_tag="HTM09_RS00040"
                     /old_locus_tag="ICBEC2AM_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            3887..4390
                     /gene="taxA"
                     /locus_tag="HTM09_RS00045"
                     /old_locus_tag="ICBEC2AM_00010"
     CDS             3887..4390
                     /gene="taxA"
                     /locus_tag="HTM09_RS00045"
                     /old_locus_tag="ICBEC2AM_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            4394..5623
                     /gene="taxC"
                     /locus_tag="HTM09_RS00050"
                     /old_locus_tag="ICBEC2AM_00011"
     CDS             4394..5623
                     /gene="taxC"
                     /locus_tag="HTM09_RS00050"
                     /old_locus_tag="ICBEC2AM_00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            5700..5993
                     /locus_tag="HTM09_RS00055"
                     /old_locus_tag="ICBEC2AM_00012"
     CDS             5700..5993
                     /locus_tag="HTM09_RS00055"
                     /old_locus_tag="ICBEC2AM_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            5990..6205
                     /locus_tag="HTM09_RS00060"
                     /old_locus_tag="ICBEC2AM_00013"
     CDS             5990..6205
                     /locus_tag="HTM09_RS00060"
                     /old_locus_tag="ICBEC2AM_00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            6561..7181
                     /locus_tag="HTM09_RS00065"
                     /old_locus_tag="ICBEC2AM_00015"
     CDS             6561..7181
                     /locus_tag="HTM09_RS00065"
                     /old_locus_tag="ICBEC2AM_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            7195..7521
                     /locus_tag="HTM09_RS00070"
                     /old_locus_tag="ICBEC2AM_00016"
     CDS             7195..7521
                     /locus_tag="HTM09_RS00070"
                     /old_locus_tag="ICBEC2AM_00016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            7540..10287
                     /locus_tag="HTM09_RS00075"
                     /old_locus_tag="ICBEC2AM_00017"
     CDS             7540..10287
                     /locus_tag="HTM09_RS00075"
                     /old_locus_tag="ICBEC2AM_00017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            10299..11015
                     /locus_tag="HTM09_RS00080"
                     /old_locus_tag="ICBEC2AM_00018"
     CDS             10299..11015
                     /locus_tag="HTM09_RS00080"
                     /old_locus_tag="ICBEC2AM_00018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            11027..12121
                     /locus_tag="HTM09_RS00085"
                     /old_locus_tag="ICBEC2AM_00019"
     CDS             11027..12121
                     /locus_tag="HTM09_RS00085"
                     /old_locus_tag="ICBEC2AM_00019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            12148..12297
                     /locus_tag="HTM09_RS00090"
                     /old_locus_tag="ICBEC2AM_00020"
     CDS             12148..12297
                     /locus_tag="HTM09_RS00090"
                     /old_locus_tag="ICBEC2AM_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            12636..13322
                     /locus_tag="HTM09_RS00095"
                     /old_locus_tag="ICBEC2AM_00021"
     CDS             12636..13322
                     /locus_tag="HTM09_RS00095"
                     /old_locus_tag="ICBEC2AM_00021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            13319..14227
                     /gene="virB9"
                     /locus_tag="HTM09_RS00100"
                     /old_locus_tag="ICBEC2AM_00022"
     CDS             13319..14227
                     /gene="virB9"
                     /locus_tag="HTM09_RS00100"
                     /old_locus_tag="ICBEC2AM_00022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            14220..15338
                     /gene="virB10"
                     /locus_tag="HTM09_RS00105"
                     /old_locus_tag="ICBEC2AM_00023"
     CDS             14220..15338
                     /gene="virB10"
                     /locus_tag="HTM09_RS00105"
                     /old_locus_tag="ICBEC2AM_00023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            15340..16368
                     /gene="virB11"
                     /locus_tag="HTM09_RS00110"
                     /old_locus_tag="ICBEC2AM_00024"
     CDS             15340..16368
                     /gene="virB11"
                     /locus_tag="HTM09_RS00110"
                     /old_locus_tag="ICBEC2AM_00024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            16355..18217
                     /gene="taxB"
                     /locus_tag="HTM09_RS00115"
                     /old_locus_tag="ICBEC2AM_00025"
     CDS             16355..18217
                     /gene="taxB"
                     /locus_tag="HTM09_RS00115"
                     /old_locus_tag="ICBEC2AM_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            18226..18648
                     /locus_tag="HTM09_RS00120"
                     /old_locus_tag="ICBEC2AM_00026"
     CDS             18226..18648
                     /locus_tag="HTM09_RS00120"
                     /old_locus_tag="ICBEC2AM_00026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            18632..18937
                     /locus_tag="HTM09_RS00125"
                     /old_locus_tag="ICBEC2AM_00027"
     CDS             18632..18937
                     /locus_tag="HTM09_RS00125"
                     /old_locus_tag="ICBEC2AM_00027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <19050..19244
                     /locus_tag="HTM09_RS00130"
                     /pseudo=""
     CDS             <19050..19244
                     /locus_tag="HTM09_RS00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            19248..19820
                     /locus_tag="HTM09_RS00135"
                     /old_locus_tag="ICBEC2AM_00028"
     CDS             19248..19820
                     /locus_tag="HTM09_RS00135"
                     /old_locus_tag="ICBEC2AM_00028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            19825..21978
                     /locus_tag="HTM09_RS00140"
                     /old_locus_tag="ICBEC2AM_00029"
     CDS             19825..21978
                     /locus_tag="HTM09_RS00140"
                     /old_locus_tag="ICBEC2AM_00029"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            21975..22181
                     /gene="hha"
                     /locus_tag="HTM09_RS00145"
                     /old_locus_tag="ICBEC2AM_00030"
     CDS             21975..22181
                     /gene="hha"
                     /locus_tag="HTM09_RS00145"
                     /old_locus_tag="ICBEC2AM_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            22199..22663
                     /locus_tag="HTM09_RS00150"
                     /old_locus_tag="ICBEC2AM_00031"
     CDS             22199..22663
                     /locus_tag="HTM09_RS00150"
                     /old_locus_tag="ICBEC2AM_00031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            22736..23440
                     /locus_tag="HTM09_RS00155"
                     /old_locus_tag="ICBEC2AM_00032"
     CDS             22736..23440
                     /locus_tag="HTM09_RS00155"
                     /old_locus_tag="ICBEC2AM_00032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            24223..24318
                     /gene="dqlB"
                     /locus_tag="HTM09_RS00200"
     CDS             24223..24318
                     /gene="dqlB"
                     /locus_tag="HTM09_RS00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            24981..25628
                     /locus_tag="HTM09_RS00160"
                     /old_locus_tag="ICBEC2AM_00034"
     CDS             24981..25628
                     /locus_tag="HTM09_RS00160"
                     /old_locus_tag="ICBEC2AM_00034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            25703..25972
                     /locus_tag="HTM09_RS00165"
                     /old_locus_tag="ICBEC2AM_00035"
     CDS             25703..25972
                     /locus_tag="HTM09_RS00165"
                     /old_locus_tag="ICBEC2AM_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            26133..26837
                     /locus_tag="HTM09_RS00170"
                     /old_locus_tag="ICBEC2AM_00036"
     CDS             26133..26837
                     /locus_tag="HTM09_RS00170"
                     /old_locus_tag="ICBEC2AM_00036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            26931..28556
                     /locus_tag="HTM09_RS00175"
                     /old_locus_tag="ICBEC2AM_00037"
     CDS             26931..28556
                     /locus_tag="HTM09_RS00175"
                     /old_locus_tag="ICBEC2AM_00037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            28604..29350
                     /locus_tag="HTM09_RS00180"
                     /old_locus_tag="ICBEC2AM_00038"
     CDS             28604..29350
                     /locus_tag="HTM09_RS00180"
                     /old_locus_tag="ICBEC2AM_00038"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_077248209.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(29741..30268)
                     /locus_tag="HTM09_RS00185"
                     /old_locus_tag="ICBEC2AM_00039"
     CDS             complement(29741..30268)
                     /locus_tag="HTM09_RS00185"
                     /old_locus_tag="ICBEC2AM_00039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(30403..30807)
                     /locus_tag="HTM09_RS00190"
                     /old_locus_tag="ICBEC2AM_00040"
     CDS             complement(30403..30807)
                     /locus_tag="HTM09_RS00190"
                     /old_locus_tag="ICBEC2AM_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(30820..31686)
                     /locus_tag="HTM09_RS00195"
                     /old_locus_tag="ICBEC2AM_00041"
     CDS             complement(30820..31686)
                     /locus_tag="HTM09_RS00195"
                     /old_locus_tag="ICBEC2AM_00041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     ARG             26932..28553
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             26935..28556
                     /name="MCR-1.26"
                     /gene="MCR-1.26"
                     /gene_name="MCR phosphoethanolamine transferase"
                     /tool="rgi"
                     /drug_class="peptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(30821..31692)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4395..5620
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          11028..12094
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          12637..13319
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          13320..14224
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          16356..18214
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggggaatggc ccctctcaca atagcccagc agggcaggag attaaaaaaa tgaatatcca
       61 ggaagcatta aacgtttttg gattatccgg cgaattaact gaaaaagata tcaaagcagc
      121 atacagaaaa gccgctttaa aatatcatcc agatcgtaac ccgttagggg ctgaactgat
      181 gaaagcggta aatgcagctt ttgatgtatt gatggcaaat attgataaaa taaatcagtt
      241 ccagagcact gatgaacatg cacgatataa ttacggtgat gacctggaaa aagtattaaa
      301 cgttctttct ggtttatctg gtctggtatt tgaagtgata ggtaactggg tctggattag
      361 tggagaaacc attacacata aggaaacttt aaaagaaatc gggtgtaaat gggcggcaaa
      421 gaaaaaacaa tggttttatc gtccagacga acataaaagt tactggaatc gtgaagaaca
      481 cacgatagaa gaaatccgcg caaaatacgg tacaaccgga cagcgcaggg cgacagggtg
      541 gcaacgcgtg gaaaccagag cgtaaccaga acgggggcga gaagcccccg atattatagc
      601 cagcccgtct tttggggcat cgaacccaaa aagacgggaa cagcagccac gtagcaaaaa
      661 gggaaacaga tgaactcttt ttttgaacaa tatcaccctg tctttgaagt tgtctgccgt
      721 atcctgggga acggctggcg cgtgaacaaa cttgatgatt gttcgtctcg tataaaactg
      781 acgtcaccgc agtttaaaaa ttactctgtg catattcgga tggaaaaaga ccgcttttct
      841 gtggtgggga gtgtggatag tcgttcctgg cgtagtccgc atcatgtttg cacgttatcc
      901 agaaaacgga atcccgttga tattgcagcc gatatcgagc gaaaaattct ggtaaatgct
      961 tcgcaggagg tgttacaggc gattgagtat gagaaacacc aggtggaaaa aaaggacgaa
     1021 attctgatcc tgaaaggtat gttatcgcaa cttgtccagc ttgaaagctg gtatggggca
     1081 ttgacaggct ttaaagctga aaacggattg aacggtaaag tcaccgaaca gggcgacagt
     1141 tatgatttac agattcgggg cttgagtata gatcaacttg ttaaaattac agggtatttg
     1201 aaacagttat gagaaaaaat aaaggtgatg tgacttattt tcttgaaaaa gaaggtgaca
     1261 attacaggct tacaaaaagg ataaaagcca gaacaaatgt taaaatcggg aacaaaacta
     1321 cgaaaattac attgtatgat gctgtactga atgagaatga attacaacat attgatttta
     1381 cgtgtgccgg attaagggaa gacgatgaga cgccagttaa gaatttaatt aaggagttta
     1441 tgttaaatga aacccgataa tacacctgaa aatgtaaaaa aattacgttt aaaagcagga
     1501 ttaacacaaa aggaatgcag tcatatttat ggtgttgggc taagaacctg gcagaaaaaa
     1561 gaggaagtta atactcaaaa cagtcaaagt ctgtcactgg ttgagtttga gtttcttttg
     1621 ttacttgcag gagaacatcc tgaatacgtg ctctgcaaaa gggagtcaaa ataaaatgac
     1681 ctgtgatggt tttatattag tacctggagt tgcgctcgta gctcgctgac gctcacacgc
     1741 gcagtacagc gccccgccat gctgcaatgc accagggcgg ggtaaactcg cttgcgctcg
     1801 cgctaatacg acttaaaggt cgtattattg tttcatcgaa aggggaatgg cccctctcac
     1861 aatagcccag cagggcagga gatttaaaat gaaatgctca tctgttttta cttcaaccac
     1921 taatcatgtt ttcacattcg aacgagtaac gctttgcact attatcctta tgcataagga
     1981 tactggacaa cagtatgttg tcatatttac tgataacaat aaaattcgtg actataaagc
     2041 cggaattgtt cctcagtttg gcgaacttaa gcagagtgat gttgatttag tccttttcta
     2101 cagggacgaa tatgaaaaat attttgattc attaaaagat ggcgatgaat gcttgagttt
     2161 taaagatttt attgagtgtc tttgttaagc attatgaatg caggggcatc cctgcatttt
     2221 ctggaaattt caatattgat tctgaaattg tctattaaca tcaatgcaag ggaaattcat
     2281 gaaaacgtta aaacaagccg caatgcagtt tgcatctgat ctgcgtaata atcgctgttt
     2341 taaagctgct tataatgatg ctctgttaga gcttgatcgg gaagatttaa acgccattgt
     2401 acaatgcaca tttctttttg agccaaaacg aacattagcc gaacttgata aattaattaa
     2461 aaagatagaa gtagaagaag attatgagtt ttgtgtatcc cttgataaag gtaaaggatt
     2521 acacgaagtc agaacattta aaagtgagcg tgaggcgttg gatgcttatc gcattttgac
     2581 tctttacgga tacacagtaa ctatagaaaa gaaggagggt aatgactgac tattcaaggt
     2641 gtgatcaggt tgttccacca acctgatcac gttatcgaca atcaaaactg aatgaggttt
     2701 ttaaatgtca acgcggcaga tcgtacagct tgctgattcc cttgtcaaag gctgggaaac
     2761 cagaattccg gcaatgaaat ttagcgaatg ggatcaattc cggtggtggt taaactactt
     2821 gcaagggtat gaaatgtttt aaataaaaaa aggggctttc tgcccctttg tattttttga
     2881 caaaaatgcc atgtgtaact ataattagtt gcatatctat aggggatgga tttatgggga
     2941 aaacagataa gctactggca aagtttttaa acagtaaaaa aacgtttgaa tgggatgaac
     3001 tggtcgtttt gttttcctct ttgggatatg tcaaaaagga aatgcagggg tcaagagtgc
     3061 ggtttttcaa tgctgaaatc aatcacacca tattaatgca tcgtccacat ccggaaagtt
     3121 atatcaaagg tggtacgctg aaagcaatta aacagaatct gaaagaggct ggattattat
     3181 gaaacattta aaatataaag gatatttagg cacggttgaa cccgattttg aaaataatgt
     3241 cctgtatgga aaactggcgt ttattcggga cctggtaact tatgaggctt ctacattagc
     3301 tgaactggaa caggagttta aaacatctgt tgatctgtat ttacagtctt gtgtggagga
     3361 tggaaaggaa cctgataccc cgtttaaagg cgtgtttaac gtcaggcttg atccagaact
     3421 gcatcgtcgg gtagcagaaa tggcgatgga agaagattta tcgttgaatg cctttgttaa
     3481 taaggctctg gaaaaagaag tcagcaatca tcgcgcaggg gcttaattgc ccttgttttt
     3541 atgccctgta agggcatggg aggcgctttg cgccggaagc cccgcagcgc agcgaggact
     3601 taaaaagcgt tacggcttca gccgtaacaa ttcccagccc tgccaggcct gttttttctt
     3661 tcacaaatct ttcagctata cgtttgcctg atggtcgaag acgaaagaat ctttcagcaa
     3721 cgaaatctgg acatatgcag cgacgatttt atagccaggc acagtgccac atatggtaca
     3781 atcaatgcag gatagacaat ccgatgtcaa atgttaacat ttgaatcaga ttgtccaaaa
     3841 acgatgtttt tgcctgtgcc tgccgcaggc tctgttgggg tgacttatga agatgatagc
     3901 gttcaggcta agtgatgatg agttaaaatt tgcagaacat aacgctattt taagtggatt
     3961 taccagtatc aatgcttttg ctaagcataa cgtgcttaat attgaaacca agcctgttaa
     4021 catcccagtt aataatgaac cagcaaagct ggtatcagtg cgtctttacc ctcatgaaat
     4081 agaacttgtt aaacgtaatg ctgctcttca cggcatgagc atgagcagag aaattgctat
     4141 tcgtgtgcgt cagtctttgt taaagagtga agtctgtctt taccctgatg aagttaagga
     4201 acttaaaaaa ctatcaactg ctgtagatcg tgttggtcgc aacattcatt ttatcataaa
     4261 aggtgaaaga ttctgtacgg ttaacgatcc agatttcaga aaagacgtta ttgaagtgat
     4321 tgaactgtgt aagcaaattg attcaaaact tgaaacgtta accaagagtg ttgttaacag
     4381 atttgggtga tttatgggtg tgtatgtaga acaggaatac cgtattaaac gcgctaaagg
     4441 gactgggcgt gatccgaaat ctcctaagtt atcgggaaga catatacatg ccagtaaatc
     4501 ggcttttaat cataaagtca gacatggtgc ggacaagaaa gcatatacct ggacacctac
     4561 aaaagaagtc acttttaaaa tcactggctc aggtaaaaca gccgcaggca tcaaaaatgg
     4621 tattgattac attacccgta atggtgaact tgaggcttac tgttatgatg gaaaaggttc
     4681 agagcaaaca gggaaaggag aagactttaa tagggagttt acttcaactt tatctaaagg
     4741 aaatgattac tccaggacat atcgtggaga aaacatcgat catgtgaaaa acatggtctt
     4801 ttcaccacca ccagaagctg gagtaagcag ggaagacgct ctgaaagcta cggtagaatt
     4861 tcttaaagag acttatccaa accatgcttt cgttgccgta taccatgatg ataaagaaga
     4921 tcatcctcat gtacatgtta atattaaact tcgtgatgaa gaaacaggaa gaagattaag
     4981 acttactaaa tctgaaacca ggaagttcag aaacggtttt catagaaaac tgaaaggtat
     5041 gggatatgat gttaccgcta cctggaaaaa agatcccgaa cgtaaacggg agattgagcg
     5101 attacaggct gaaaatccga aaagactccg gaatgtttac aaggtggtgg attttgggga
     5161 gacttcatat cagaataagg caggggaaaa acgaacacct ttcctcactt atgaaacttt
     5221 aaaaggtgga aaacaggtta cgatttgggg taaagacctg aaaaatcatt tcgagtctga
     5281 aaagctccag ccaggaacgc ttatcaaagt aaaaaaactg gctcctacgc ttgtacgtag
     5341 tccaatgttt aatgatgatg gaactgttgc aggctacagg gaaacgcatc gcaataactg
     5401 gcagattgaa aatattgccc ttgagcggaa cagagaacgt caggttcatg agcgtgaaac
     5461 caggcagccg gaagagagcg acgttaaaaa gcagctatcc agaaaacatg agcaaggtca
     5521 caatatcggg tttgctcttg aacacgggtt tacaaaggat tcagaagaac ataaaaaact
     5581 aagaatacag caagagagga actggaaagg cctgggattt taatttgatt ttggcgcagg
     5641 tgaacaaagc ggctactttg ctcacctgct gatcacaaca acctacgagg gaggttatca
     5701 tgactgagca tgatgctatc tgcatttctg gacttcatca aatttttagt gatgaagaac
     5761 atttgagtga gcaacaaaaa gacattattc tgatgtacgc ttatggctat accctgaatg
     5821 aaattgctga tttcaaagga ctaaagcctt caactgtcag aaaatatctc gattctgtaa
     5881 gagctgaact gggaggtgtc tcgttggcag ggatcagaac actggtactc attcggacta
     5941 atgcccttct cgtgtcaagt ctttccagga tcagtgagag ggggaattta tgaaaaaata
     6001 tctgcttttt gctttgccgt tcttcgttgt tggttgtagt gaagaagtaa aaagtgtgga
     6061 ttggtggggg cagcatctta cggaagctaa acaaaagcag gcagagtgtg agaagtctgg
     6121 ctctgattct cagaactgta agaatgttaa acaagcattg tttattcagt ctcagaagga
     6181 tgctccagtc cctacattcg attaactaat gtttcaatca tactagagcg cccatatggg
     6241 cgcttttttt tgcatgatca cacgaatcat aatatgacca ttttgtcaat gttatttgat
     6301 cgtattgttg agcattgtta ttttgcgaat catgttcgca tctaaaacaa atccaaaaca
     6361 cacttatagt ggattgatta aatgaaggag aaatcagata agtcatcgga aaaaggggtt
     6421 tatgtcacga taaaactcga tcccaggttg aatgcattta tcgaagagaa aacgaaaaca
     6481 tctttgctta gtaagaggaa gcaaattatt tacttattaa tgcagagtat gcgtattgat
     6541 ggatatatgg agcgttagct atggcgtctt tattcccagc cttgttgctg tgtgcggcgt
     6601 cagttcatcc ggatacgata aatgatattg cacgggtcga gagtggcttc aatccttatg
     6661 ctgtggctga aattattcct gaaagggaac gcggtagcta tggcaaaagt gttatcagtc
     6721 atatgcccaa aagtaaggag gaagctctaa cagttatcag agaaattgaa aacagaaaaa
     6781 gacgttattc tgtcggattg atgcagatta ccagcagtaa tttttctttt tattccacgt
     6841 ccgcagaaaa actccttgat tcgtgtgaaa acttgtctgt tttcgaaaag attatcgtgg
     6901 attgttataa acgagggagg agcctggaga atgcgcttag ttgttactat acaggaaatt
     6961 ttagtaacgg taagcgaaaa gaaaaagaat ttaataatac aagttatgtt gagcgaattg
     7021 gttatacagg gaacgaaaaa aaatatgttg taccaggtac aagaagcaac gggggagaac
     7081 agcgaaaaaa tcgttcacac aatgcgtcag ttatctggcc cgaaaccatt ctgaaatctg
     7141 cttttgttga taactcacat ccaacaaaag ttattaatta acaggactta taaaatgtca
     7201 tttaagaaat cagtatgtaa tcgtatcagt tcatctgtca ctgctgtaat gcagcgtaaa
     7261 aataaggtag ttatggcagt cgccggggca acagttgcat caccggcctt tgcggatgga
     7321 ttttcaaaag cggaaacact gctggaaaag gttaaaacag ggcttagtgg cctttcactg
     7381 gtcacggtga ctattgcctg tctgtgggtc ggctacaagg ttctgtttgg tggaagcacg
     7441 attcgtgaat gctcacctat catcatcggg gcaattgtta tcgccagtgc ggctgaagtt
     7501 gcatcaatga tggttaacta ataaagcggg gacgatagta tgtctacact ttataaagcg
     7561 atgacacgcc ctgctatgta tgtgggtgtt cctgtcgtcc ctcttactgt ggttgcaggg
     7621 gcgttatttc tggcgggggt ttatatcagc aagctgatct ggctggctat tcctgtcgcg
     7681 gtttttgttc tcagaatgat cactaaacag gacgaccaca tttttaacct ttactttctt
     7741 aagctgaaaa tgctcggaaa ctcagtgtgt aatcgttttt ttggcgcacg agcgttcctc
     7801 tccggacaat atgaagctgt tgaaattgat gagtttgtta atgccatgaa actgaatgag
     7861 cgaatcacta ctggtaaata tataccctat tcaagccatg ttgataaaaa tattgtcaaa
     7921 acaaagaacg gtgattatgt agcgacatgg caactgatgg ggattaactt tgagtcaatc
     7981 tctgcggaga tgttagaaac tattgactca caggttgcca ctcttgttcg ttcattttcc
     8041 ggactgcccg tttcatttta taaccattca tgcagggcga gtttttatga tgcctttacc
     8101 acaaaatcag gtaataaata tgctgatata atatcagatt gttactatgg ttcgatgaag
     8161 aaaaataaat tcaaggggaa cactctttac tttacgctga tctacagacc agatgggcgt
     8221 gtagaaaaac tggaaaaacg caaaaaaagt attaaagaaa agaaagatga tattaacatt
     8281 catgtgaaga gaatgaatga aatgattaat acattctctg gcgctcttga taaatttacc
     8341 tgtaaacttc ttggtatgta tgaggagaac ggaaaggtat tttcttctca actgtcattc
     8401 tataattatc ttctgacagg gaagttgcaa aaaatcagag tcactgattc tccggtgtat
     8461 aacgttcttg gtggagtgga tgtatttttt aatcatgata ccggacagat atgccgcatt
     8521 gatggaaata aattctttcg ttctattgaa attaaagatt tctgtagtga gactgcatcc
     8581 ggtgtttttg atgtgctcca gtattctgat gctgactata ttattacgca ttcatataca
     8641 tccatgagca aatcagaagc attgagtacc attaaacgtg cggaaaaaca gttaaagagt
     8701 acagaagatg atgcggttac tcaattgcag gaactggaaa aggcaaaaaa tgatattgtg
     8761 tccggtgata tatcctttgg ttactaccat ttcacgctaa tggtaatggc ggacagtatc
     8821 agggagcttg acgaatctgt aagtaaaatt acggcagact ttactgacct gggcattatt
     8881 ccggcattgt caaccatgtc attgccagcg gcatattttg cccaacttcc ggcggttttt
     8941 catttaaggc cgcgtttatc tccggtatcg aatgtaaatt ttgttgaact ggcatcattc
     9001 cataatttct accaggggaa acgcgataaa aactgctgga cagaagcggt tgctattctc
     9061 aaaacaccaa gcaaacaggc gtattatctg aatttacata actcagtact gtttaaagat
     9121 gaaacaggtg aaaagaatct tgctaatact aaagttatag gaacggcggg ttcaggtaag
     9181 acaatgtttc tgtcatatct tgcctgttca ttacagaaat acaataaccc cgaaactttt
     9241 gctgattctg caaaaaataa gaagttaacg tgtgtctttc ttgataagga cagaggggct
     9301 gaattatgta tccgaatgct tggtggggaa tattacaccg taaaaagcgg tgagccgaca
     9361 ggatggaatc cttttgctct ggaggcaaca aaacgtaacc gtatttttgt caagcaactg
     9421 atggagattc tgtgtaccag aaatggtgaa agattatcga caagagaaag attgcttatt
     9481 agcgaaagtg ttgatgctgt gatggatttt cctcctggcg aaatgcggga atatggcatt
     9541 accagaatgc ttgagcatct tatgcagaga gatgatcggg atgagcagga gaacgggatt
     9601 atccttcgtt tgtcccaatg ggctaatggt caggctcatg gctgggtttt tgataatgct
     9661 aaagacacat tcaatattca gcatgtcaat aattttggca ttgatggcac cgagtttctt
     9721 gatgatccga tggtatgtgc accaattact ttctatctgc tgtatcgcat tacgcagctt
     9781 ctggacggtc ggcgtcttgt tatcttcctg gatgagttct ggaaatggct acaggatgaa
     9841 gcattcagcg actttgtcta taacaagctg aaaacaattc gtaagctgaa cgggctggtt
     9901 attccggcaa ctcagtctcc ggatgaaata ctgaagaata agatttcacg ggctgtggtt
     9961 gaagtatgca gcaccagtat ttatctggct aaccctgatg ccgattacaa tgattatgtg
    10021 gaagggctta agctgacacc agaagaattt aatattgtta aaaatcttga tccaatgtcg
    10081 agacagttcc tgattaaaaa aagcagtctt aaaaaggggg atggtaaatc attttcagca
    10141 cttgcgacac tcgatttatc gggacttggt ggatatctga aaatattgtc tgccagtgca
    10201 gataatctgg aaatttttga aagtatttat catgaaggaa tggagcctga tgactgggtt
    10261 cctgaatatc tcgaacgggc aatctgacag gtgaatttat gaaacgtgta aaaacattga
    10321 tgctgatatc attattgacg gcttcatttt atagtagcgc aggaattccg gttgctattg
    10381 atgccagtcc ggaatgggct gttgaagccg caagatggac agaacgactc aaacaatggt
    10441 ccgaaactgc gcaacattat cagagtcaga ttcaggcata taaagaccag ttagccacgg
    10501 caacaggtat aaggaatatt gcggcattca caaatgaact aagtaatcta caatcagaac
    10561 tgacaaatat ttataagcag gggaacagtt atatttctga ttttaccagt aatcccgaag
    10621 gtgcattgag cagccaggct aaaaatctgt tcagcaagta tggagccttc gatatgtgta
    10681 atacgggcta tgaacgtaat gataatctct gtaaagcacg gattgtgtca acggctgcca
    10741 gtattgaaca gggaaatgag atcaataagc agttatcaag tgcaatgtct cagattcaga
    10801 gtttaagtag ccgaattgag gcttcaaagg atatcaaaga atcccaggat ttggctaacg
    10861 cattacaggc gcagtcgtta aaaatgcagg cgataaaaat gcagtatgat gtctggaata
    10921 ataaaaataa agcagatcat gagatgttgg tcacacaaga acaagaggcc tttattaaac
    10981 agcagaaaga agctgaacca ttaacttttg attaaaaggt ggatatatgg ctcaggggtt
    11041 tttcgttaaa tataattcta ccgttatgga ttcggtagat aaaatcagct ccagttatca
    11101 aacacaattt gctaatgata ttatgtcgtt agcaacggta tcggttacgt tatatgtact
    11161 atggaaagga tatcaaatac ttgccagtaa aactcagacg cccttgcagg atttagtatg
    11221 ggatctatca aaatttgcaa tcataatcat gtttataact aatgcggatg ggtatttaac
    11281 tgcggctact gatgcgttgc aaggcatgaa agatggtttt tctggagggg tgagtgtctg
    11341 gcaaacttta gataacttat ggaaaagtac gcaaaatctt ggtgcggaaa tatactcttt
    11401 agataaatca acatatgtta aagatcaagg tgtagttggt cagtttttaa tatggactgg
    11461 ctcgcttatt ttaatggctg ttagtgtggt tgttttttta acagcagatg taacgatgaa
    11521 actcctgatt attacagcac cgatatttat cttttgtctc atgtttggtt ttattcggag
    11581 catgtttaat aattggttac aatcgttatt ctccagtatt ctgacagttc tatttgccag
    11641 tttagtgatt cggatagcaa tggattttca agggatgatc ctttcccatg ctattcgtgc
    11701 tgcacaaaca gggaatgtaa atcttgtatc tacaggtgct atgggcttta tggctgggtt
    11761 tttgggggct ttattggttc ttatagctaa aggttttgct gtccagttag caggtgcagg
    11821 tgttgaaggt gctgttcagg gcgcagccat gatggggctg ggtgctgctg gtatggctac
    11881 tggtaagtct ctgatgcttg gcggtcgtgc tggtttaggt tttggcttag ggatggccgg
    11941 aagaacgggc atgaattctt tatcagggaa agcagggaat ctgatggggc gtggtgcgcg
    12001 aacagccgct gaatgggcgg gtgaaaaggg atatcaggcg ctggctccga caggtgcttt
    12061 ggggatgaag gcgagaagac ttgcatccct ggaaaaagca cgagcgagga acgccgcatg
    12121 aaaataatga aacgtttttt ttatgtgatg tccctgaact ggatactggt gttcgttgat
    12181 aaacatatca cctccggatg gagcaggaag agaaagctaa taacccagct tgttatttta
    12241 atcacgggaa ttaccttatt taaggtctta ggtggaatca gtcagagctg gttgtgacat
    12301 gcttcattat tttgtcatga tactttattt tgtcctaatc cttcttttag gagtgtttac
    12361 ctgcttcgca ggaataagac ttggtttagg gcttgcgggg aaatggagaa agggtatgga
    12421 tcttcgcttt ttgcttctta ttggtgggat tctttttctt catcttggcc tttatctctt
    12481 aaaggtgttc gtgaacgctt aataaggtga atttatgcga tgtgtgattc ttttgttttt
    12541 cctgtttcag atttccggat gtaccacaag gaataacctt ccttctgatg tttccggcga
    12601 actggaaccc attaaccaca gccaggtaat cagttatgag tgaaaaaatc gaaaagaaga
    12661 ttgagacagc taaatccttc gaaagacaac tctatgcaga aaatgaacgg tctaagaaaa
    12721 atgcatggcg tgttgcagtg gcagcatcat tactgtcctt atgtcttggg gctgcaattt
    12781 gtgttcttgt gccattaaaa gaaaaagagc ttgctattgt ggctgttggc gaaaaaacag
    12841 gccgtacaga actgattact caggttaaac aggaaaagat cctgcaatct gaagcacttg
    12901 ggcggtattt tgtaaacacg tatattactt tacgtgaagg ttataattat ccgtctcttc
    12961 agtatgatta tgagactgta cagctatata gctcgaatac agtaaaggac gattatcttc
    13021 gtctgtataa cagcgatatg gctcctgata aaatttatca taataacggt tcttacgttg
    13081 cggttgaggt catctccaat attatttctg acgcaaccgc gccagataaa ctggcctctg
    13141 tgcgctttaa aaaaacgacg agaaacttca cgaccggaca ggtttcagtt tcatactgga
    13201 cggctcgtgt gacatatcgt tttgaaccag aaaagtcggt taaaagttcc agtcgtgagc
    13261 ttaatcctct cggttttact gtaaccagtt accagacaga tcgggaagtc aggggggaat
    13321 gatgaagaaa ttatttattg ctacatgttt attgcttcct gggcttgtgt attcagcagc
    13381 gactccgtta ccttctggat ttgatgcaag gatgcagact gtgagttata acggtgcgaa
    13441 tacgaccgtt atccggtcaa agacaggatt tctcacatca gttgtttttg atgaaggtga
    13501 ggctgttatc agtgcaaagg ctggttttcc tgccggatgg gaaatcacaa ccgatgataa
    13561 tgttgtttac atcaatccgc gtcccgttgt tcaggaacag gaaggcgatg aaggcgaaaa
    13621 actgaaaaag gttttccagc caacagaaaa ggaatgggat acgaatcttt ttgtcagaac
    13681 aacaaagcgg atttacagtc tggatctaat ccttctgtca gaagaaaaac aagcgcaacc
    13741 tgcttatgtt gtacaattcc gctatccatc agaaatagcg aaaaagaatg cagaagaagt
    13801 aaggctggca aaagaaaaac aggaaaaact cagacagaag aaactgattt ctgaaagttt
    13861 tgaaaaagcc gatgcgccta aaaactggaa ttatttcatg cgagtcaatg aaaaatatga
    13921 cagtcgtcgt atcgcccctg attttgctta tgataatggc atatttacct ttttaggttt
    13981 taactcaggg aaagtattcc cagctccatt tgctgtaaga gatggtcagg aacagacact
    14041 tgcttttaat gtggaaacaa aaggcaagta taaaattatg gttatccaca acgttaatga
    14101 caaatttgtt ttacgctatg gaaacagcgt tgttggtgtt gtgaataaat ctttcggaaa
    14161 agtattaacc gaccagagga atacatcttc tcctgcggta gaacgtgttg aggtgaataa
    14221 tgactgatat gaaaaaaaca gatgatttag ttgatgatgc gaatcagtca aagctggaaa
    14281 gtaaacccgt tcatgatatc ggtgatatca ggaacaaaaa taatcgtatg cgttctggct
    14341 ttctgtttgg tctgatggct gtattagtta ttggtatttt tgcgctgaaa acatataaaa
    14401 attattttgc agatgaccag cagaaagcat cagaatcaac aggcgatacc agcatttctc
    14461 aggtaagtaa aatccgtact ggtcttggtc agaattttga ccctgttgag aacaaagcaa
    14521 ttatcaatcc aggcgctact ggttctggta atactgtttc tgacggtcat aaagaagtgc
    14581 cacaggaggg gttcaggaaa tatttgtcaa tacctgttgc tggtcaggga gggagtcaga
    14641 ctggcgcgag tggcagcagc aggacatcgc agacatcaga gccacaggaa gaaaggaaag
    14701 aatcaaaaga aaatgtgcct ggtaaatcag gaatgaaagt aactgcgatt aatctcgatc
    14761 ctgatctcta tattgaggaa aacaggctta taccgtgtgc tttaaccaca cgctttgttt
    14821 ctgatgttgc aggtcgtatc agttgtgttt ttacagaaga tgtatggagt gctaatcacc
    14881 acacaaaatt actggaacag ggaactaagg cttttggtcg ttaccagacc ggaacgctga
    14941 atcatggaca gggcagaatg ttcgttatgt gggagcagtt gcggacgcct gataataaac
    15001 gaatcgatat ggtaaataca gcagcagctg gtcccctggg agaagcgggc attgatggat
    15061 ggatcgattc tcacttctgg gagcgttttg gtggttctct tatgctcagt atggttcagg
    15121 atgtagcggc ggcggcggcg gataatgcac ctggtaaaga tcgtaatgtg gattataccg
    15181 aaaactcacg acaggcaatg gctgaaatgg ctaaagtcgc acttgagaat tcgataaata
    15241 ttcctccaac aatgtataaa aaccagggtg acataatcac tattatggtc ggggaagaca
    15301 ttgatttttc tgatatctat gagttaaagg taaaatagta tgagtggcgt tattcctgtc
    15361 agcattagca ataaatcgct cgatttttat aaaaatcaag tattccagtc ttttcttgat
    15421 attgaagggc taacagaaat tgctgtcaac cgtcctggtg aaatctggac ggaaattaat
    15481 ggtgactgga cattccataa taatgacagt gtgacctttg atttttgtaa acgtttttct
    15541 catacgctgg catcatttcg gggagatgaa atcggcgaga ccaagccatt actttcagca
    15601 acactggaat caggagaacg tgtacaggtc gtttttcctc ctgcatgtga gagaaatact
    15661 atttcaatca ctatacgtaa accatcgaca cggcagataa cacataaaga atatgtggcg
    15721 aacggttttt atgattatgt ccagtcagga aaaaaacaca ggacatacga tgatgagctt
    15781 cttgatttat tcagagcgag aaatatcgct gcttttatgg agcttgccgt agcagcaggg
    15841 aaaaccattg tatttgccgg agcaacgggt tccggtaaaa caacttatat gaagagtctt
    15901 atcgatttca taccattgaa tacacggctg attacgattg aagatgcaga ggaaatcaaa
    15961 ttttttatcc ataaaaatta tgtgcatctt ttttaccctt cggaatccgg cagtgattcc
    16021 ggctctatca taacagcagc gaaattaatc aaatcctgtt tgaggatgaa acctgacaga
    16081 attttactgg cggaaattaa aggtggcgat gcctgggatt ttgtgaaagt tgctggttct
    16141 ggtcacagtg gaagcatgac gtccattcac gcggcctcgg caaaagatgc catcattcag
    16201 atggtgacaa aatgttatca gaacagagaa tgtcagaatc ttccttttga tgttcttcgt
    16261 aaaatcatta tggacagtat tgatattgtt gttcatgtag gcagggatgg tgttgtcagg
    16321 catatgagcg acatctatta caaaggggct gaatgtgaaa acttttaata agtcgcaatc
    16381 tgtttttata ttcgctgtta tgcttgttgc ggcatggttt gcaggttcat ttattttctt
    16441 tgtgctttac ttcagccaga taaagaatct gaaaccattg aaggctgtgt tcagatccat
    16501 tgatgcgtac aggatggata tttttacaga cagccttact ttatctgttg tgactacgga
    16561 tatcagagtt ttagcctttg ttgcacttgg agcaggattt gtcgtgtctc ttatcatccc
    16621 tgttgcggcg ttgataaaac tgaacgagaa gaaagaaaat attttcggtg atgcgcgatt
    16681 tgcaacaatt aatgatattc gtgagtctaa cagtttcacg cttgacggtg acgaaaaaga
    16741 cgggattatc gttggtataa aagacaagaa aatcatacgc tatgtgggag cagcattcag
    16801 tgctatggga gccggaacaa gggctggtaa aggtgctggt atagtcatca ctaatcttat
    16861 gaagtactgg tggagtgtta ttattctcga tccaaaaagg gagtgtttta atatcaccag
    16921 tcttatcagg aaggtgattt taggtcatga ggtttataaa tttgatccct ttagttcggt
    16981 aacgcaccgc tttaaccctt tgtattatgt cagcatggga acgagtgaag gttttaatca
    17041 gcttgaaaat ctttcgttga ttatttaccc ctataagaca gatggtgcgg atgctggtag
    17101 ctatcttaat aagacggcgg gtggtgtttt taaatcttat gctgtcgctt tatggtttat
    17161 gattaagaat gataaggctg gactaaaaac gcttgatatt gagcctgtct tctcaatgtc
    17221 taaaattaat cagctttttg agcgtgcaga acccgaacat cttctttcat tcatgaatga
    17281 tattcgttca gaattaaggg ggaaagacaa aacgcttgct gatattggtg tcgctggcct
    17341 taaggcattc attgagatgg aggataaaac aaaagcacaa ctcaaaagca actttcttaa
    17401 tggattatca ccgtttgcta accccaatgt ggcaaatgct actgatggaa atgattttga
    17461 tttgcggcag gtgcggaaaa agcggatgac aatttatttc tgtatttctg gtgataatgc
    17521 caggttggct gaaaaaataa caaatatttt ctttcagctt gctatacagg tgaatcttga
    17581 aaagatgcca acggatgatc cggaaataaa gcatgactgc ctttttctac tggatgaatt
    17641 cccgtcaatt ggtgctgtag attacatcaa gagtaaatct ggtctcattg ctggttacaa
    17701 gctgaaattg ttgattgttt accaggtcgg ctctcagctt gaggaaatct acagttatgc
    17761 aggcagtaaa acattactgg caagtgcacc ctgcaaaatt gtttattccg cttctgatgt
    17821 taaagatgcg cgggagttat cagaagcgat ggggacacga acagtaacta tcggttcgaa
    17881 aagcaaaagt cgtagtcgtg gaggaacatc gagatctgaa tcagaaagcc ttattgagcg
    17941 tccactggta acaacaaatg aactacttac actgaaattt agtgaagaaa tcctggcaat
    18001 gaagggagaa aacccgattc gttgtgaaaa ggcattctac tatctcaatg agtatttctt
    18061 tggtgacttt gtgaaggttg ctccggaact tgcaaacatt ttccccagga agaaaggaaa
    18121 gctggttatg cctccacaga aagtatttga aaatgaaatc gtcgcaaaag ggtatctggt
    18181 agtaaaagat gttcctgatc tcgataaagc ggcataaagg agaacatgat gaagaagtta
    18241 gtgtttttat tgatttccat tcttgctggt tgttcatcac cgccagagcc aacacccgtg
    18301 cagtttgaaa aggcaaatga agtgatcaat ccctcattgc catatgtccc cgattttcac
    18361 ggtgtcataa agtcagacgt tagcggtaaa ggatgggttt atgagatcac ttccttatcc
    18421 ggagtacagg ccagaacgcc gacgttttat tacgctctgg ctcatgcgga caggattgta
    18481 gtgacaacac atgatgctgg gctgtggttt cgaatcagag atctgttgaa gatggaaggg
    18541 gcaacagcag tcgtggaatg gcgaaacgag aaaagttttt tacctgaaca ggtaaaaata
    18601 gttttcatta aagcacaaaa tgaggtgaaa aatgactgga aaaaatagag tggttttacc
    18661 agccttgctg gcgttaggat taatatcaac aaatgctaat gcatctgacc cttgcgcttc
    18721 ggtactttgc ttatatggaa aagcagtcgg gcaaggtgga gggagtgaat gcagaagtgc
    18781 tgaaaaggat ttttttaata ttctgaaaaa gaaaaaagga agcattcgct ggagtaaaac
    18841 ttttgatgcc cgtaaagcat ttctgaatca gtgttcaacg gctgatcctg ctgctatatc
    18901 aaaaatcatg agtaagtttg gtcgtgtcag aggttaatta aaacctgata accgcgccaa
    18961 cgggtatcag ggtactgcaa gtatcacatt tcgcgccaac gaaatgtgat gattagttac
    19021 aatcactgag gttagaaatg aaagtaacca aatctattat agctatttta ttgtgcttat
    19081 ctgctggtag tgcataagtg gatgtaataa aaaatgctcg tgcctttttg aaaggaaccc
    19141 tcgatctgag cgttcaggaa gcagatgaag atatagagga aagggaagaa ctttataaaa
    19201 agaacggggc acaacctgac tatttaagtt acctggagga ttaacacatg caatatgcat
    19261 tgtttgatgg atttgaacga aagtttttgc tggatgctct tgaattcggt gttctgaagg
    19321 actggaaaga aaatccggta aaagaacttc ctgatattga tgaatctgct caccccttcc
    19381 atatctgtta tggtggatat ttattaaacc ctggtgtttc agattcagat attagcagaa
    19441 aaataaaaga ccaggcagga ttctggctgg cagctattga tgatacccgc atggattgtc
    19501 attcaatagc ttattatgat attcacacac tccctctgat ttcgtgtggt catcaaaaga
    19561 tagttccttt tgccgcgtta ataaaagctg atgaatgcat catttcaaaa attgcttcgt
    19621 attctggttt tgctgtaaca gcctttttga gaattaaaga gtgggatatt gcaactaata
    19681 tacttaaccg tgagggaatt tttgccttta atggctgtga gcgcagattc agagtagtaa
    19741 gtaaagataa ctggcaacat acagtatcgg aagaacgcgc tatccgttgt gccaaaagat
    19801 taattcaatg taaaggataa caaaatgaga ctttttatcg cagaaaaacc cgcagtagca
    19861 aatgatattg ttaaggcact tggtggcaat tttacccgcc atgatggctg gtttgaaagt
    19921 gataacgcca ttgtgactaa ctgttttggt catattatcg aatcacaacc gccggaaaac
    19981 tataatcctg aatacaaagc ctggaaggtt gaaacgcttc ctttacgtct ttatcccgtg
    20041 aagtatcagc ctgttgaaag tgcagcaaaa caggttaaaa cgattctcga acttatcaga
    20101 cgtggagacg tgactgaaat tgttcacgct ggcgatcctg atgatgaggg acagctactg
    20161 gttgatgaag tcctggaata tgcaggcaac acaaaacccg taaagcgcgt tctgattaac
    20221 gacaacacgc ttccggcagt gaaaaaggca ctggcaaatc ttaaagacaa tcgtgatttc
    20281 aaaggacttt accttaaggc gttggcgcgg tcagttgccg atgccgtcta tggcttctct
    20341 atgacacgtg cgtacaccat tccggcaaaa gccagaggat atcagggcgt tctgtctgtc
    20401 gggcgcgtcc agactcccgt tcttggcctg attgtgaatc gtacccgtgc taaccagaac
    20461 cataaatcca gtttttacta caccatgacc ggagtctttc agcgtggtgc tgatgttatc
    20521 agggcgaact ggaagccagg ggaatttgct ccgctgacag accgtaaatt acttgataaa
    20581 gcgtgggcag acggaacggc tgcatccctt gcaggaaaac cggctacagt tgaagcagca
    20641 gcaactgatg ataaaaaaac ggctgcgccg ttgccgttta atctggtcag actccagcaa
    20701 tacatgaaca agaagtttaa aatgacggca caaaaaacgc tggatattac gcaacaacta
    20761 cgcgaaaaat ataaagcaat tacgtataac cgctctgact gctcatatct ttcagatgaa
    20821 caattcagcg aagcgccgca ggttatcgat gccctgaaat cagtctttcc tcagtcgctg
    20881 gatattgatt ctgcacgtaa aagcaaagcg tttaacagtg caaaggtgac tgcgcatact
    20941 gcgataatcc cgaccgccag tgtgcctgat gttaacgcac tcagcaccga cgagcgcaat
    21001 gtttacctgg cgatcgcaca acactatctt gttcagttca tgcctgaaaa agcataccag
    21061 gaagtatcgg ttgccattca gtgtggtgat gagtcgttct atgcccgtgc cagaaaaaca
    21121 actgacagcg gatttgaggc gtttcttggc gcggaaacca cagacgaagg tgaatcagaa
    21181 gataatgatg attccgcttt tgaactgctc tgtaaaattc gcacaggaga aacactgacg
    21241 acaaaagaag ttattgttaa tgagaagaaa acaacaccgc cgccgttatt caccgaagcc
    21301 tccttgcttg ctgcgcttgt tcgtgtcgcg gattttgtca ctgatccaac aattaaaaaa
    21361 ttgttgaagg ataaggataa agacaaaaaa gatgaacatg gtggcattgg tacgccagct
    21421 acccgcgcag ccattctgga aacgctgaag aaaagaaact atatcacgct ggaaaaaggg
    21481 aaacttattc ctactgatac cggatatgcg cttattgatg ccctgcctgg tatagcggtt
    21541 aatcctgata tgacagcatt atggtctgaa aagcaggctg ccatagaaaa tggcgacctg
    21601 acggttgaac agtttattaa tgatctgtac ggtgaactga caggcatgat ttctgatgtt
    21661 gacctgggcg agatgaagat tgaacccgct gcgccagcag ggcagtttca acgcctggac
    21721 tctccctgcc cttcctgtgg taaacatatt gttatcaggc cgaaaggtta tttctgtacc
    21781 ggatgtgaat ttaaaatctg gagtgagttt tctggtaaga aaatcaccca ggcacaggcc
    21841 gaaaaactgg ttaaatcagg gaaaaccgat ttgattaagg gatttaaaaa gaaaagtggt
    21901 ggaacgtatg atacagttct tgtccttgag gataagaaaa cagggaagct gggttttccg
    21961 gcaagggcta agaagtgaaa acaaagcagg aatggctttt tcagttaaga aaatgtacat
    22021 caagagatac tcttgaaaaa gttattgaga ttaaccgtta caaactgcct ttatcagaat
    22081 cagaggcatt ttattctgcc gcagatcatc gccgagcaga actggtgatg aataaacttt
    22141 atgataaggt gccttccggc gtctggaagt acgtacacta aataagagga ttaattatat
    22201 gagcgaactg actaaagaag atgaatacgg cattatcagc cgtactatga tgaatattcg
    22261 ttcattgcgt gtttttgccc gtgagattga ttttgagcag ttgctcgaaa tgcaggaaaa
    22321 gctcaacgtt gttattgaag aacgtcgtga agatgctgaa cgtgaagcgg ctgaacgcca
    22381 ggaacttgaa gccaaacgtc agcaggccat tgaatacatc atatctttag gtcttgatcc
    22441 cgaatcactc cttgctcctg taacagcaga tacagtaaaa accaggcgga aagccaaagg
    22501 cggagtaaga aaggctaaat accgctttaa ggatgaaaat ggcgagatca gagaatggtc
    22561 tggtaatggc aagcgtccgc ttgctttaca gaagctgctt gatgaggggc atttcatgga
    22621 agatttcctc attgagaaaa ctaagccgga acaggcagaa taacccgcca ggggcactgt
    22681 tgcaaatagt cggtggtgat aaacttatca tccccttttg ctgatggagc tgcacatgaa
    22741 cccattcaaa ggccggcatt ttcagcgtga catcattctg tgggccgtac gctggtactg
    22801 caaatacggc atcagttacc gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt
    22861 cgatcactcc acgatttacc gctgggttca gcgttatgcg cctgaaatgg aaaaacggct
    22921 gcgctggtac tggcgtaacc cttccgatct ttgcccgtgg cacatggatg aaacctacgt
    22981 gaaggtcaat ggccgctggg cgtatctgta ccgggccgtc gacagccggg gccgcactgt
    23041 cgatttttat ctctcctccc gtcgtaacag caaagctgca taccggtttc tgggtaaaat
    23101 cctcaacaac gtgaagaagt ggcagatccc gcgattcatc aacacggata aagcgcccgc
    23161 ctatggtcgc gcgcttgctc tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca
    23221 ccgacagatt aagtaccgga acaacgtgat tgaatgcgat catggcaaac tgaaacggat
    23281 aatcggcgcc acgctgggat ttaaatccat gaagacggct tacgccacca tcaaaggtat
    23341 tgaggtgatg cgtgcactac gcaaaggcca ggcctcagca ttttattatg gtgatcccct
    23401 gggcgaaatg cgcctggtaa gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa
    23461 ggctgcctca tcgctaactt tgcaacagtg cctcacacag acatacacat aggtatgtct
    23521 gtgtgacata cctatgtaca gtgccagatg gcgttgctac gtgaaaaata acctacccta
    23581 cccctgcttt tttcgcagat tttacgtagc acaccgttgc tatagcattc atctaaaaag
    23641 ctgaatgtcc ttttctgtcg ctgttgcatt ttaattcact gaaaaatatt gagtaatgaa
    23701 aatgccggta acgcgtcaag ctgttggcca acgcgacagg cagtattgga agcaacaaaa
    23761 aaaatgaaag aaaaatccct caaatgaagg agaaaaaacg gaactacagc cgaacgacag
    23821 gcacaaaaaa acccgactgg taatcgggct tttttgcgtt ttccagtctt gagttggtgg
    23881 ctctgaccgg agaaggttac tctacaccta acgtttacat tttattcact atggtgctgt
    23941 aaagcaacta aatgtggtta aggaaccaca tttagtggtg taacgggtgt tttgtgtagc
    24001 gtataatcag tcagttagtc atgtagttgg cttgcaatcc tgctgttccc gtatctgcgg
    24061 gtatgaacga ttttaaagtc ctggctggat aaacaggtgg tgctgtattc ggtgctctgg
    24121 caggaggagg tcactctaca cctaacaatg cgagagagcc gttgtgacag gcagtgtatg
    24181 agcaagcaac ggtgatttcc tccagtttta ctggagaaac ggatgattaa tatgttaatc
    24241 atcgttctca gagctgtggt cgccgttgca aacgcgctga ttgcagttct ggaactgatc
    24301 cgccaattca tcgattgatg acaacggaaa cgtaactaag ggcaggaagt gaaccgcttc
    24361 ctgcccttta acaaaaaagg aggatttatg gattttatga gcattttggg gcgctgttta
    24421 cttcttcctt tccctgttgc gatgtttgtc ggttgcctgt ttcctggcat cgatgatcgc
    24481 ttatccggaa tgcttatgag tgttgctgtg ggatgtttat cgtggtatgt caccagaccg
    24541 aagaaccgac agacaaaggc cgcgtaagtg gcctttaatt gggataccct ttagttacaa
    24601 cattctaata gctcattcca tcctccgctc atccagcaaa cataacctcc ctgcccctcc
    24661 ccactacaga aagtggcgcg ccccgcccgt acgggcggaa tctaatcgct taattttgaa
    24721 tgttgtaact aaacatactc attgctgtat tgccacaagg tttcgcagca agggaggata
    24781 ccgaaagtaa cgctgtcact tttgctttca agtgaccaac caacgggtac atcacactga
    24841 catacaataa tgtatttctt catgatgtac atcattgtaa ttcattgtga catacactat
    24901 agtatgtcca tgtgacatac gcatgcgtac atcacataga aatacacatg agtaaatcac
    24961 tgtgacatac aaggggtact atgattatcg taatcggtgg tgataaaggc ggtacaggta
    25021 aatcacatct ggcaaccaac ttaactgttt gcctctcaca acagggaaag agaacggggc
    25081 ttgttgaaac agacctgaat ggctcgacca aaaaatggaa taaacgcaga gaacaggccg
    25141 gattaccgcc cgtcgcatta aatgaagcct atggcgacat cagtgccaaa ataacaaaaa
    25201 tggcagatgt agcagaaatc ctcattatag atactgccgg atatgacagc acggagttca
    25261 gaactgcact aaaagttgca gacattgtga ttgtcccgat cgacccgctt gcccaggtgg
    25321 aagcagacag tcttcagact gtgacaaaaa ttgtcaggga tgcgcaaaaa ataaatccca
    25381 ggctggctgc acatgtgctc ctgtataaat gccagccaaa tacgttcagt gaacaacaag
    25441 aattaagaga ctctctcaac agccatgatt actggcttaa accaatgaaa agcacggttt
    25501 cttttctccg ggcctttgta agagcaatga atcagggaat gggggttcac gagttaaaaa
    25561 gtaatgttag cggtgccagc caggcaaaag cacaaatcga actcttgctg aaagaactgg
    25621 aactgtaaat catagtgaca tacactaatg tacatcatca tgatatacat tagtgtgaaa
    25681 caatcagatt agaaggtaca tcatggcaaa gctggaacta ccagaactag aacaaaacca
    25741 ggaagtgaac gccacaaaat ttgttcagga cgcaggaaaa cgccccacag agaacaaaac
    25801 aaaactggct tcattccgca taccgaatga attgcttgaa ttcattgata acgaatcaaa
    25861 aagactgcat cgtcccaaga caaaaataat caaagcagca ttactggctt atcaggatct
    25921 ggacgaaaac gaactcatta atttgtggtt cagagctgac agaaatgatt aatcatagca
    25981 agtggcccaa taaactcttt atcattttct tttagatact tatctgaatg tactaacttc
    26041 atcactttta aatgtagttg tgattcattt ttagttactc ttgcaataaa atacccttgt
    26101 acagttgttg cacgggtatt taggagcact agatgagcct tatcgattta agtttatcag
    26161 gattatcaga accaggaaca aaacttattg aaaaaataag tgatgctatt ggtgtgcttt
    26221 atgaacctac aagaatcagg aaaaaagcga aggctgaagc tgaagctaaa cgcacagagc
    26281 taatttctag gttagagctt gagggaatag aaaaacgggc agttgagcgt tttctcaaac
    26341 gtgaaacgaa acgacaggaa aacattgaaa atataacaat gcaagcagca caaagtctat
    26401 ctgagagtga taatgtttct gatattgacg aagattggat agaggctttc ttcagggagt
    26461 gtgaggatat tagtgacgaa caaatgcaaa tgctttgggg aagaatttta tccgaagaag
    26521 caaagtcaaa aggttcgttt agtcgtagaa ccttaaaact attatccact ataagcaaag
    26581 aagaagcgaa ccttattact tattttggaa agtttgtttg gcaggcaaac aaactcactc
    26641 caattttatt taccgatgag aatggagata ctgaaggtat aactttcgat aaactttcgg
    26701 ttttagactc cttaggcgtt attcaacaag gcattggtta tagtttacaa attataaata
    26761 ctctcaagtg tatattcagt atgggattgc gcaatgattg cctaataaaa tttctgaaat
    26821 atttctgtat cgcataattt tttatatcag ataaattgta ctggatttct taaaaaattg
    26881 cagtataatt gccgcaatta tcccaccgtt tattttttga gtagtttctc attatgcagc
    26941 atacttctgt gtggtaccga cgctcggtca gtccgtttgt tcttgtggcg agtgttgccg
    27001 ttttcttgac cgcgaccgcc aatcttacct tttttgataa aatcagccaa acctatccca
    27061 tcgcggacaa tctcggcttt gtgctgacga tcgctgtcgt gctctttggc gcgatgctac
    27121 tgatcaccac gctgttatca tcgtatcgct atgtgctaaa gcctgtgttg attttgctat
    27181 taatcatggg cgcggtgacc agttatttta ctgacactta tggcacggtc tatgatacga
    27241 ccatgctcca aaatgcccta cagaccgacc aagccgagac caaggatcta ttaaacgcag
    27301 cgtttatcat gcgtatcatt ggtttgggtg tgctaccaag tttgcttgtg gcttttgtta
    27361 aggtggatta tccgacttgg ggcaagggtt tgatgcgccg attgggcttg atcgtggcaa
    27421 gtcttgcgct gattttactg cctgtggtgg cgttcagcag tcattatgcc agtttctttc
    27481 gcgtgcataa gccgctgcgt agctatgtca atccgatcat gccaatctac tcggtgggta
    27541 agcttgccag tattgagtat aaaaaagcca gtgcgccaaa agataccatt tatcacgcca
    27601 aagacgcggt acaagcaacc aagcctgata tgcgtaagcc acgcctagtg gtgttcgtcg
    27661 tcggtgagac ggcacgcgcc gatcatgtca gcttcaatgg ctatgagcgc gatactttcc
    27721 cacagcttgc caagatcgat ggcgtgacca attttagcaa tgtcacatcg tgcggcacat
    27781 cgacggcgta ttctgtgccg tgtatgttca gctatctggg cgcggatgag tatgatgtcg
    27841 ataccgccaa ataccaagaa aatgtgctgg atacgctgga tcgcttgggc gtaagtatct
    27901 tgtggcgtga taataattcg gactcaaaag gcgtgatgga taagctgcca aaagcgcaat
    27961 ttgccgatta taaatccgcg accaacaacg ccatctgcaa caccaatcct tataacgaat
    28021 gccgcgatgt cggtatgctc gttggcttag atgactttgt cgctgccaat aacggcaaag
    28081 atatgctgat catgctgcac caaatgggca atcacgggcc tgcgtatttt aagcgatatg
    28141 atgaaaagtt tgccaaattc acgccagtgt gtgaaggtaa tgagcttgcc aagtgcgaac
    28201 atcagtcctt gatcaatgct tatgacaatg ccttgcttgc caccgatgat ttcatcgctc
    28261 aaagtatcca gtggctgcag acgcacagca atgcctatga tgtctcaatg ctgtatgtca
    28321 gcgatcatgg cgaaagtctg ggtgagaacg gtgtctatct acatggtatg ccaaatgcct
    28381 ttgcaccaaa agaacagcgc agtgtgcctg catttttctg gacggataag caaactggca
    28441 tcacgccaat ggcaaccgat accgtcctga cccatgacgc gatcacgccg acattattaa
    28501 agctgtttga tgtcaccgcg gacaaagtca aagaccgcac cgcattcatc cgctgatttc
    28561 tccctgtatt ttttccaaac ccaccgcaca ctccattcgt attatgggcg gtggggtggg
    28621 gtttgttatg ccgtatttat caaataaacg cctacttgct gagatgagta tcgctcttgt
    28681 catggcgatc gttgccacgc tgacccttga gcacagtcag attgatctga tggtcgctga
    28741 ttggttttat ctgggtatgg ggcattggat ggttgccaag caagcttttt tgccagattt
    28801 gctactgtat tctggactaa aaaagctgct gatggcgatg ctgatctact tgctggttgc
    28861 gaccatttgc cgtgcttatc atgagaaaaa gggcaatgct atcactgcca agtggcttgt
    28921 cccagtgaca aaatttcgcg tgcgtgagct tgcgtatctg gtgctgactt tgatcctagt
    28981 gccgacagtt gtcgcgtcat tgaaggcata tactcatgtg gtctgccctg tgcatttgac
    29041 gatttttgat ggtacgctgc cgtatttgcc gatgcttgat agtatgcgta acaccattcc
    29101 tgataagtgc tttcctgcgg cgcatgccag tagcggattt gcgctgtttg cctttgcgtt
    29161 tgcgccaagt ttgcgccgcc gtcgtggtgc gatcatcatc gtggtgatgg cattgggctg
    29221 ggcgatgggc tgctataaga tgattattgg cgatcatttt ttgagccata cggtggtgtc
    29281 gatgatgctt gcgtgggcga tgtcggcagg gcttgcgtgg gtgtttttta agaagggtga
    29341 acaagtttaa tcgcctttaa aagtaaaaac ggtcgtatcg gttattacgg aacggttttt
    29401 tcagttgagt ttcaaagcga tgattcatca acctggaaac taccaatagg aacggcttta
    29461 cttacgccta tcggtcaaga attgatgaaa atatgcggtt ccactccaga tattgcgtat
    29521 ttaaataaat tcctcaacaa aataaatgtt gaaggaagcc aggtaaaact atcaattatt
    29581 aatatgtaaa gaggtcgagt tacctgtctg agttccacta accataatta aaagcgaccg
    29641 caaaaactgc ccagtgaaaa ctgtgttgcc agtaatcaca aatgcagcaa gtactaataa
    29701 agagcctgtt aagcccgcaa atatgtgggc ttatttttaa ttactcttca tcatcccgca
    29761 ggaactgaca aaaatcttcg ctccccatca tatcgacagt gtttttaaat tcgaaaaaag
    29821 aaattcctgt catagcacac acgcgctgga taatcagttt tttcttttgc tgtctgacgg
    29881 ttgcggtttc accaaactgg ataacagcca ggactgtttc atcatatgat ttcaggacaa
    29941 aatcacaggt tatattctgc acttcacgga ttaaatcttt tacagcttcc tcgctaccat
    30001 gaggacgaac aaattcaatg agccgtattt tgggaaaaac ggaataatca ggtataatat
    30061 tacgtgaaag aatgcctaaa aactctgatt cattatttga gagtaagggt actttagtaa
    30121 aacaatcaac ctccccaatg gctgtaaaag cattggttgt caaagctgga ttattcttaa
    30181 taaaatcaga gaatacattt tcagctgatg gcaaaacaac agcagaatta tgttgttcat
    30241 ctcgtttttt attacggtta aatatcattt catattcctt ttaaaagagc ctgcggcagg
    30301 cacaggcaaa aacatcgttt ttggacaatc tgattcaaat gttaacattt gacatcggat
    30361 tgtctatcct gcattgttct ttactgtctg taagatcggc gctcattctt ccggcgttct
    30421 cccgatacca aaaagacgga acgcaaaacg gctttcgatc caccattcaa aaatctgctc
    30481 tgcgcaacca caaacagtta aggcgatcca tgcagcaaga acaacgtaac tactgacatc
    30541 tgcacccatg taccaggcga tagttaaacc catcactgcg ataccgccta aaaactgcaa
    30601 caagaaacga aatcccgcaa gaatccccaa tggaataaca acaaccagat aaagggaagc
    30661 agccagaagg tatctgacag gtgtcagcag ataaaagaac ctggatattt tctttttgtc
    30721 agaataaacg gcatcatttg caacatcggt tgaatgatca ttcatgctgt ccctgattct
    30781 ttgaaaatct acgatattat ccttcatatt gttatgacct caattttcag gtgggtacat
    30841 tttatcaaac atattagtaa actctgagtc ttccctggta tcgccctcat aaccaattgt
    30901 ataagagaac tttaatttat aaacttttct cccaattttt tctgaaacta cgaaacttaa
    30961 gttctttact tccgtatgtt tcatgatttc ttttactgat ttattaagaa catctctttt
    31021 aaaagcagga taatctggat acttataatc cttcaccccc cccgccccta ttgtataaag
    31081 attcaattca tctttaagtt catctattgt catttcaaaa ctattcgcgc gagaattatt
    31141 actatatatt ttacgaatga gttgatatag tgatgcagca ttaacacttg tcagtcgaac
    31201 aacagataat aaaacttgcg ttgtataacg tttttcttca ccaataagtt tacataaaaa
    31261 tcttttcgct tctttcgtaa acttgatccc cactctacct tcattcctgt aataagtaga
    31321 aaaaacagtc agactcaaat tcattgaatc aggtacatta cttttagtga acttaaatcc
    31381 caaatcacta ctcagttcta atatttcatc atgtttcagc ttaagaagcg tcgtatctaa
    31441 tatattagag ccatccctca aagcaagata agccgcatca ggcttaacct gaacccattt
    31501 aatgtaatcg gcaactgtca cataaaatat gtgatcatca tcgaattcat ttttagagtt
    31561 tatctggcac atggctaaaa ataagacttt tttagcagcc ataggcaatg ttgacagcgt
    31621 tgaattcaat tcatttcgat gtctcacttt tgttaagcga gaaagggaag tcttatttgt
    31681 cgtcattctc attgttccaa cactcttgat aatcagactt taacaccaga aacatgatat
    31741 tacaacacaa tcaatatcat gtttaaccga gtatcttctc atgtttcata tctgtgattt
    31801 atctcacaaa ggtaatccat gctcaaaatc cacactggat tcaaggcaga ttaacaacag
    31861 attcaaaata gattcaccag aaagaacaac gaattagtat tcttatgaca cattttgtgg
    31921 atatcaccga ggaagctctc atgtttaacc gaggaagctc tcatgtttaa ccgaggaagc
    31981 tctcatgttt aaccgaggaa gctctcatgt ttaaccgagg aagttatcat gtttaaccga
    32041 ggaagctctc atgtttagac aaaaaacacg cttaaaatca ataaattaga gctactctaa
    32101 gtttttaagt ttttaagaaa aacaagaaaa gataaggctt ttaatgcgtg cgaatattca
    32161 ggaactgtgg ataactcaac aaaaccagca acgatgatgg ttccttacca taaatctgtc
    32221 ccccgatgat tattacccgc aagctaaaac aaacatcatc aatcattaaa actcataact
    32281 ttcaataaca taaaatcatc aaaaaaatat ttgatgcctt atgtatctca attttcaaga
    32341 ccataaattt gtccctagtt aactttaact tattgaaata aatataatat tcgtgcgtac
    32401 tgatcctaat gtcacatcgc aaaaattccg cttcctcgct cactcagtcg ctacgctcct
    32461 gccgttcggc tgcggcgagc ggtatctcct ttctcaaaac cagagagatt cagatcatat
    32521 gatccgttat ttgatccttt ttggatcttc cactaagaag ccctgaatcg cgctctgccg
    32581 cttcgttcga tacaaccatg aggaaaacca tgcgaaatga aaaaaatcgc ttaaaatgcg
    32641 attcagagcg ttttagggag gttctgttag ttctcctgca ttgcgcccgt agttcgctaa
    32701 cgctcacacg cgcagtacag cgccccgcca tgctgcaatg caccagggcg gggtaaactc
    32761 gcttgcgctc gcgctaactg cggcattctg cggcgcaaat cagcataatc atggcctgct
    32821 ggtcagttgg gctttgcagg gtgaagggat ggcctgtgca ccctggtacg ttcgtcagcg
    32881 gctgcgccgc gcttcggggc gatgtggctc ctgtgctgcc ctggtatttt cgttcgcccc
    32941 ttccatccgc ttacgcggat taaaaaacgt gataacaatc acttatcgag taaaaaaacg
    33001 ggagaaaact tgcaaatacg acttaaaggt cgtattattg tttcatcgaa a
//
