LOCUS       NZ_KY595965             3221 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Enterococcus faecium strain VREfm1 plasmid pPEC013, complete
            sequence.
ACCESSION   NZ_KY595965
VERSION     NZ_KY595965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227885
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Melegh,S., Nyul,A., Kovacs,K., Kovacs,T., Ghidan,A., Dombradi,Z.,
            Szabo,J., Berta,B., Lesinszki,V., Paszti,J., Toth,A. and Mestyan,G.
  TITLE     Dissemination of VanA-Type Enterococcus faecium Isolates in Hungary
  JOURNAL   Microb Drug Resist 24 (9), 1376-1390 (2018)
   PUBMED   29750597
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Melegh,S., Kovacs,K., Nyul,A. and Mestyan,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2017) Department for Medical Microbiology and
            Immunology, University of Pecs Clinical Centre, 12 Szigeti Street,
            Pecs 7624, Hungary
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: SPAdes v. 3.8.1
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/06/2024 04:14:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KY595965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VREfm1"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pPEC013"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            84..803
                     /locus_tag="HTL36_RS00005"
     CDS             84..803
                     /locus_tag="HTL36_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002311815.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLKK
                     VRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINK
                     TKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVR
                     IEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFS
                     SLTSDLPKISLRNWLEE"
                     /name="replication initiation protein"
     gene            complement(940..1356)
                     /locus_tag="HTL36_RS00010"
     CDS             complement(940..1356)
                     /locus_tag="HTL36_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001232859.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKIEA
                     KLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(1359..1643)
                     /locus_tag="HTL36_RS00015"
     CDS             complement(1359..1643)
                     /locus_tag="HTL36_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000353844.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESDF
                     DRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
                     /name="hypothetical protein"
     gene            1988..2233
                     /locus_tag="HTL36_RS00020"
     CDS             1988..2233
                     /locus_tag="HTL36_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002311817.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMT
                     PERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
                     /name="hypothetical protein"
     Typing          85..803
                     /name="rep_cluster_1763"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatccttttg tataataaca accaacctat ttacgagttg gttgttaaag gttattattt
       61 atttgtaagg ggggaagaaa ggaatggatg aaaaaacgga attaacagtc aaatatcaaa
      121 atgaattgaa tttagtaccg ctgaaaaatt tcaatgctaa gcagatggat ttattttttg
      181 cattatgcgc tcgtatgaaa gatcgtggtt tgaaaaaagt tagattttct ttcgaggaat
      241 taaaagagtt aagtgattat aagatgactg ctacaaaagc ctttgtcggt gatttagaaa
      301 agctctataa agatatgctt aatcttactt accgtactga agatgaggat tctattcgct
      361 actttgtttt gtttactggt tttgaaatca ataaaacaaa aaagtttgtt gaagtcagtg
      421 ttaatccgga cctagaatat attattaatg gtttaacagc tgaatttagt agatttgaat
      481 tatctgcttt cacgtcgttg cgttctacgt atgcaaaaac tctattccga ctgcttatgc
      541 agtaccgttc tactggttat tatgtagttc gtattgaaga gtttaggagc ttaatggata
      601 tcccagaata ctatcaaatg ggtaacattg atcaaaaagt attaaatcct gcattacgag
      661 aactaaaaaa atacttcaat gatcttaaag tcacaaaaat aaaagcaaaa aagggcaata
      721 aaatcgccaa attagaattt actttttcaa gcctaacgtc agatttgcca aagatttctc
      781 tgcgtaactg gttagaagaa taaataaaaa agaactcgcc ccccttacat taagcaagta
      841 gacaagtccc ttacaaccga ctaagattgc tagtacattc tagcatattg tatggggctt
      901 ctaaataaaa aacagatagg aataactcct atctgttttt tatcgtttta gaaaatttgt
      961 tgggtcgaaa tttctagaag tgactgtttg actggtttct ttttgatgat tctttggtcg
     1021 atttcgattt gatggattta gtttagcttc gattttttct ttcacttctt gatatacttt
     1081 agaatttgtg ccaataagaa cgcctgtatc ttttagatat aaatcagcaa attctgcgat
     1141 gatttcagtc atgatggaaa tttcttttcc catagcattt agttttgcct gattgctggt
     1201 agtggtcgtg ttggtatcgg gttcataact catacataaa aagcgaatgg cttccgagta
     1261 gttattgata tctggatttt tttcgatgat ggaatctaaa acgtcaacat gactagggta
     1321 caaagtaaca tgtttttgga cagaagtttt tctcatggtt atatcattcc tccgataagc
     1381 atttcaatga tttgattatt tgtgtagaca tactcgctta atatttgagt attttctgtt
     1441 tggatagcaa taaatttatc gagcaataaa tcgaattggt cttttttgta agtcgtaagt
     1501 tgacggtcaa aatcactttc gattaaaact tttagatatt tatttcgact ccatttttct
     1561 cctgttttat tcgtgagttc ttggcaacgt ttgtctattg ttgcaacaaa ctttggatca
     1621 atattccgaa ctaaaatgtc cataaaaaat ctccttttgc aattatgtat aatttatgta
     1681 taccatacaa atactatatt tgtatagctg ttttttgcca aaatgctata cataagctac
     1741 ccacgatggc gaaaaagtga tatcaaattt ttttctgaga aacgtgatag gcacagccta
     1801 tctacccact ttttttagaa agtgggtaca cgtttcctta tgctctttaa aattcggggg
     1861 ggagtgctgc acgtgtaatc gataaccaaa tttaaaaatg cacgatcaaa aatttattta
     1921 gtttttcaaa cgacattttt tttgagagta tagacggcaa agaagtgatt ttatttctag
     1981 aaaaattgtg agactggaga aagaaaaaat ggaaaaattt ccttacgaat attttcaaaa
     2041 cttagaaaaa ttagagcaag caattcaagc aatcaagttc gatttaacag acgaaaaatt
     2101 gttggaatat aaaaatatga cacccgaaag aaaacgatcg ttggaaaaat taatgcaact
     2161 agtgaacgaa gacggcggaa tttcagacgc agcttttgac gcatggagcg aatttcatgg
     2221 agaatataat taagtgggtt taaatggctc taaaatcgct tctaagagtt ttttagcttg
     2281 gaccaatgaa atacaccaaa aactaaataa aacgtcttaa atcgaaaaat acgagctctc
     2341 agagcgactt tatttgtaaa tgttgcatat aagaaataat tacggatttt agacttttgt
     2401 aggatatttt tgaaataaat atttatttct gttataattt gattgcaaat aagtgaacac
     2461 gaaacacacg aaagccccag agaggtcgga ttctctgggg ctttttttgt gggctgagtc
     2521 gccttaatta agttttactt cttgtttcgg ttattcaacc agtaactgaa caaagcgctt
     2581 aaaagcccaa caagaaggga gcaaataaag ttagtgaaca cgaaacacac ctccttccag
     2641 tcgcaagact gtcggatagg cgacaatcaa ttatacaata aaaaagcaaa aataagccta
     2701 agttttttat tgccttgggg ttgttagggc ttgccctgac cgcctgtaag gcgctttaga
     2761 acgaaggaca tagtttttat cgaatcttgt cttagaacgg cttagacgag ctctcaggac
     2821 gccacaaagg agtccctaat gaattatttt tggcgaagga gcctaaaggc gactgctgca
     2881 agtaaaatgt gagagcgtag cgatcactcc ttcttacagc gaccataggg agctagattg
     2941 gcagtttaat atcatgcgcg aacctgttta ttttaaaaag tgggtgattt tttggcggga
     3001 aattttgctc gcgcgcgcgc gtttatttta tttatttttt aaagattttt tagaaccctt
     3061 tttagggggc agttgagcct tactctccca acggatacag aagggtacta acaacttttt
     3121 atctgagtac taacaacttt ttatctgagt actaacaact ttttatctga gtactaacaa
     3181 ctttttatct gagtactaac aactttttat ctgagtacta a
//
