LOCUS       NZ_KY595964             4465 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Enterococcus faecium strain VREfm1 plasmid pPEC012, complete
            sequence.
ACCESSION   NZ_KY595964
VERSION     NZ_KY595964.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227886
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Melegh,S., Nyul,A., Kovacs,K., Kovacs,T., Ghidan,A., Dombradi,Z.,
            Szabo,J., Berta,B., Lesinszki,V., Paszti,J., Toth,A. and Mestyan,G.
  TITLE     Dissemination of VanA-Type Enterococcus faecium Isolates in Hungary
  JOURNAL   Microb Drug Resist 24 (9), 1376-1390 (2018)
   PUBMED   29750597
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Melegh,S., Kovacs,K., Nyul,A. and Mestyan,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2017) Department for Medical Microbiology and
            Immunology, University of Pecs Clinical Centre, 12 Szigeti Street,
            Pecs 7624, Hungary
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: SPAdes v. 3.8.1
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 03/06/2024 04:14:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KY595964.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="VREfm1"
                     /isolation_source="wound"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pPEC012"
                     /geo_loc_name="Hungary"
                     /collection_date="2012"
     gene            complement(663..1097)
                     /gene="bacA"
                     /locus_tag="HTL66_RS00005"
     CDS             complement(663..1097)
                     /gene="bacA"
                     /locus_tag="HTL66_RS00005"
                     /inference="COORDINATES: protein motif:HMM:NF041453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin 51 precursor BacA"
                     /protein_id="WP_002351079.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="bacA"
     gene            complement(1502..1675)
                     /locus_tag="HTL66_RS00010"
     CDS             complement(1502..1675)
                     /locus_tag="HTL66_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010729871.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYNS
                     PNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1752..1919)
                     /locus_tag="HTL66_RS00015"
     CDS             complement(1752..1919)
                     /locus_tag="HTL66_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172689816.1"
                     /translation="MVKIPLIRENGQEPKRSAKKTYQAPQAKKVRVTSKKEKFFRTTFE
                     NLILIYIRSI"
                     /name="hypothetical protein"
     gene            complement(2360..2899)
                     /locus_tag="HTL66_RS00020"
     CDS             complement(2360..2899)
                     /locus_tag="HTL66_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002351084.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINET
                     GQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQEK
                     DNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNTQPL
                     IEKKWWQIWKSNRTNL"
                     /name="DUF536 domain-containing protein"
     gene            complement(2915..3838)
                     /locus_tag="HTL66_RS00025"
     CDS             complement(2915..3838)
                     /locus_tag="HTL66_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002351085.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEIIF
                     SFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDKQL
                     VIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKEKFF
                     DLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTWKAE
                     INHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQQQQTE
                     EKQAEKRELSNKLKQDLLGNLQNLFD"
                     /name="replication initiation protein"
     Typing          complement(2916..3838)
                     /name="rep_cluster_1291"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcaaattag agcttgataa agatatttgc tcgtgtgcct agacttttaa aaaagtcggc
       61 acacgccatg acaaagctga aataaactgc ttcagctttg tcatggcgga ttcgctcact
      121 tgaaaaagtg tttatttcgc tcgattatta gcatatattt tttagctttt ttgctagcca
      181 aattttgcat taatttgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
      241 tcgtaatgtg aaagtacctt taaaggtact ttctacaaaa ctcactaaaa gggtttggca
      301 agctctatat ttgctagcca aatacctccg tatttggtgc aaataaccgc ttgtcaaagg
      361 ggcgcaagct cccccttgaa tccccctcaa aacccacgct ccacaataca aaaagtggct
      421 agaaaatgct agccactttt tgtaaaattt agtatgattt atttttagct atttagagtt
      481 tttatttccg taaatacttc taaaaattgc taagataaat gctataggaa ttagataaag
      541 aatacttttt tgtaagctta tattgaaaat tagcctaaat atgaagaaca tagtgattaa
      601 aactattatt gaaataatta cagctaaaat ctttttattc atgagatagt ttcactttct
      661 gattatttta ttacataacg agcgtaatca ttgtattgat atgatgttgt attgctaaag
      721 tgagcattat atgttaattg tacacctttt ccttgatttg cagctcttac aaagacattc
      781 attgcatcaa cagcactact aaaagcattt gtaataaccc atgttccaac tccaccaata
      841 ggaataaaag atgcaatttg agcaggagtc tttagcttat tataattttt tttcaaatct
      901 ctacacattt tattagtaac ataatagttc attttatagc aagtaaatcc acgatgatta
      961 tgattatatc gtgaacttgc agcttccact tttatacctc tagcactaaa aggaaacaga
     1021 tttaacggag tacttgctcc aacaatcata ccgaacaaaa atagacaagt aataatttta
     1081 ataattttat ttttcattgt tacttcctcc ttagatataa aatcctagga aagcgctttc
     1141 tgtatatatt tagaataatc agaaaattaa atattgtcaa gagggtttat tttataaaac
     1201 gtgttatttt aaaggttagt agacataatg tgtactatgc caagtagcct ctctaccaag
     1261 ggatacaggg gctcactttg tgattttcga ttggtctaag tcaaaagctc tttttaaggc
     1321 ttgattttct gcagttgctt ctttccgttt ctttgtgaat ttttaccagt cattttttac
     1381 tggggtgtta tttttcataa aatatgataa atttttggta gcgcttcata agcgcgttgc
     1441 tttttcaaga ataaacactc attatttttt aactggctgc tcgaaagagc agtctttttt
     1501 attatttatc cgtctctttt acaaacaatt cttcgttagg actgttgtaa taatttgaat
     1561 ttaatttaag ttctccgttg gaattgtttt ttccaattac ggataatagc atggggctaa
     1621 aacctaatat tcctagagaa atagaaccca gtaccaacac agtttttttt aacataggtt
     1681 atacctccac tttttttaac gctaccactt gtatacacat tttaaatact agccttcagt
     1741 ttatcaaatg tttaaattga acggatataa atcaaaatta aattttcaaa agttgttcta
     1801 aaaaattttt cttttttgga agttactcta actttttttg cttgtggtgc ttggtatgtt
     1861 ttcttcgccg accgtttagg ttcttgacca ttttctctta taagtgggat ttttaccacg
     1921 ttgagagaaa atggtcaaga acctgggagg cggagaatgc ccttaatacc aagcaccgca
     1981 agcctttccc atactttatt cgatgatccc acgatgatct cataatgatt ctacaatgac
     2041 cctacgatga tcaaaattct caaaaaaaat aaacgaaatt tcaaacgatt tttttgaaat
     2101 tttgttaaaa tcacatttgt ttttttatga ctttttcaac aaaatcgatt agaatttatt
     2161 ttgtttttcc accaaaaatt gagtgagatt tcaacctatt ttaagcaata agtcaccata
     2221 tagtttgata gtttacaact ataaacattg attgtttaca gttgtaaact attttttctt
     2281 tctcataaaa atttgagtga tctccccatc aggtagtaca actttttcct ctactatccg
     2341 aactaacttc tagaaaattt tagagattgg ttctattaga tttccaaatt tgccaccact
     2401 ttttttcaat aagtggttgt gtattgtctg cttttttttc agaaaaggca gttgattcgg
     2461 ttgttggagt ttcttctgta taagttaaat ggagctgttc ttgtaggttc tcaatttgtt
     2521 ggttcgcttg tagagtgagt tgttgttgct ggtcgagtaa tttttgcatt tgttttattt
     2581 gattatcttt ttcttgtagc tgtatatctt ttcgatttaa ctgtttttct aggaaagaaa
     2641 ggagttttaa ttcggaaact tggttggtgt ttttatggtt tttggttttt gatttttcgc
     2701 caactagttt tcgatttaag tttgcgttat cgactaaaaa cattgatttt atagcttttt
     2761 gaccggtttc gttaatcaca aacttgtttc cgttttttgc aaaccaaagt tttcgatttt
     2821 tgtcagaaac ttttttgtta attgctgttt tactaactcc taattcttcc gcaagttctt
     2881 tgattgtttt atctgccata aatactcctt aagcttaatc gaataagttt tgtaaatttc
     2941 ctagtaaatc ttgtttgagt ttgtttgaaa gttctcgttt ttctgcctgt ttttcttcag
     3001 tttgttgttg ttgtttaaaa tcagcttcat gagtgcctaa gggaagattt agtattcgat
     3061 ctttagcgcg ccatttttct tctcgtgtta attctccatt gtgatcaata ttaaataatt
     3121 ttatacgtaa atcttcttgt ttccctttgg aaaaatcgtc agcatggtta atttcagctt
     3181 tccaagtaaa acgataaccg ataacaggtt tacctcgacc tttaccatat tttttacgaa
     3241 tagttaatcc tttaaataag ggagtgagtt cttctttaat tggccttaag ataaatttgt
     3301 caatatttga cggactattc caataacttt taggaatatc tagtaaatca aaaaattttt
     3361 ctttggaaaa ttcagtccag ccttgagttc gatattgttt taataatcga aacatagttt
     3421 ttgaatatgt gctatccaaa ttagtaaatt gctctagatt aaatcttgtc cagttcttta
     3481 attgattaaa aagtccttta aattctggat taactgaaat tataacaagt tgtttatctc
     3541 gtataatgct atattttgtg aaagcaacaa atttagttaa agtatttcca tcatcattcc
     3601 aagcattaat acttaataat ttgtcatacg tgctactaag atcttttata aattgttccc
     3661 cacgttctgt ataaccgctt agttttttca aatcgtgaaa tgaaaaaata atctcatcta
     3721 cacctttttc acgtacacgt gaagcaattg aaaaaaataa gttcatttca cgactattaa
     3781 attttctcag aggaatatga tttaatctat tttcatattt tacaatttca tttgccataa
     3841 gcttctccta cttttataaa gtatgaaata aaagtaacat actttataaa aatactcaat
     3901 aatgtcggaa aacctacttg aaaagcgtag aaaacctact tgaaaagcgt agaaaaccta
     3961 cttgaaaagc gtagaaaacc tacttgaaaa aactctgaat tgcttgggag agtaaggaca
     4021 cagccgacat aaaagagata taaaaagaga tataaaaaga gatataaaaa gaggggaggc
     4081 taattttccc gcctaaaaaa tcactccttt tttctctaaa caggttcgcg aatttatcaa
     4141 aactgccaat tccaaacctt gctccctacg gttcgctaaa aaggtgtgat cgctctgctc
     4201 tcacattata ctgcgcagtc gcctccggct cctttgctaa aagccgttca taaggaaccc
     4261 tttgagaagc tctgagcgtt cgtttaagac attttaagac agtcctagta taaatccctt
     4321 atcttgttct taaaacgcct tacaggcggt cagggcaagc cctaacaacc ctaaaattgt
     4381 ctaaatgccc tctaaaattc gatttaagca ctcttaaacc tattcttata tatttatata
     4441 cttaggcgtt tcaaacgtct taaaa
//
