LOCUS       NZ_KY463454            33309 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain WCHEC050618 plasmid pMCR_WCHEC1618, complete
            sequence.
ACCESSION   NZ_KY463454
VERSION     NZ_KY463454.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN06197016
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     The complete sequence of an mcr-1.4-carrying plasmid pMCR_WCHEC1618
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Zhao,F., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.0
            Coverage                          :: 100x
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009973095.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 02:01:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 40
            CDSs (total)                      :: 40
            Genes (coding)                    :: 39
            CDSs (with protein)               :: 39
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KY463454.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="WCHEC050618"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="pMCR_WCHEC1618"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2015-11"
     gene            731..826
                     /gene="dqlB"
                     /locus_tag="BVJ50_RS00200"
     CDS             731..826
                     /gene="dqlB"
                     /locus_tag="BVJ50_RS00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            1489..2136
                     /locus_tag="BVJ50_RS00005"
                     /old_locus_tag="pMCR_WCHEC1618_00002"
     CDS             1489..2136
                     /locus_tag="BVJ50_RS00005"
                     /old_locus_tag="pMCR_WCHEC1618_00002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            2211..2480
                     /locus_tag="BVJ50_RS00010"
                     /old_locus_tag="pMCR_WCHEC1618_00003"
     CDS             2211..2480
                     /locus_tag="BVJ50_RS00010"
                     /old_locus_tag="pMCR_WCHEC1618_00003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            2641..3270
                     /locus_tag="BVJ50_RS00015"
                     /old_locus_tag="pMCR_WCHEC1618_00004"
     CDS             2641..3270
                     /locus_tag="BVJ50_RS00015"
                     /old_locus_tag="pMCR_WCHEC1618_00004"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            3444..5069
                     /locus_tag="BVJ50_RS00020"
                     /old_locus_tag="pMCR_WCHEC1618_00005"
     CDS             3444..5069
                     /locus_tag="BVJ50_RS00020"
                     /old_locus_tag="pMCR_WCHEC1618_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            5117..5863
                     /locus_tag="BVJ50_RS00025"
                     /old_locus_tag="pMCR_WCHEC1618_00006"
     CDS             5117..5863
                     /locus_tag="BVJ50_RS00025"
                     /old_locus_tag="pMCR_WCHEC1618_00006"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_077248209.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(6254..6781)
                     /locus_tag="BVJ50_RS00030"
                     /old_locus_tag="pMCR_WCHEC1618_00007"
     CDS             complement(6254..6781)
                     /locus_tag="BVJ50_RS00030"
                     /old_locus_tag="pMCR_WCHEC1618_00007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(6916..7320)
                     /locus_tag="BVJ50_RS00035"
                     /old_locus_tag="pMCR_WCHEC1618_00008"
     CDS             complement(6916..7320)
                     /locus_tag="BVJ50_RS00035"
                     /old_locus_tag="pMCR_WCHEC1618_00008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(7333..8199)
                     /locus_tag="BVJ50_RS00040"
                     /old_locus_tag="pMCR_WCHEC1618_00009"
     CDS             complement(7333..8199)
                     /locus_tag="BVJ50_RS00040"
                     /old_locus_tag="pMCR_WCHEC1618_00009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            9614..10129
                     /locus_tag="BVJ50_RS00045"
                     /old_locus_tag="pMCR_WCHEC1618_00010"
     CDS             9614..10129
                     /locus_tag="BVJ50_RS00045"
                     /old_locus_tag="pMCR_WCHEC1618_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            10234..10776
                     /locus_tag="BVJ50_RS00050"
                     /old_locus_tag="pMCR_WCHEC1618_00011"
     CDS             10234..10776
                     /locus_tag="BVJ50_RS00050"
                     /old_locus_tag="pMCR_WCHEC1618_00011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            10773..11024
                     /locus_tag="BVJ50_RS00055"
                     /old_locus_tag="pMCR_WCHEC1618_00012"
     CDS             10773..11024
                     /locus_tag="BVJ50_RS00055"
                     /old_locus_tag="pMCR_WCHEC1618_00012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            11011..11238
                     /locus_tag="BVJ50_RS00060"
                     /old_locus_tag="pMCR_WCHEC1618_00013"
     CDS             11011..11238
                     /locus_tag="BVJ50_RS00060"
                     /old_locus_tag="pMCR_WCHEC1618_00013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            11773..11991
                     /locus_tag="BVJ50_RS00065"
                     /old_locus_tag="pMCR_WCHEC1618_00014"
     CDS             11773..11991
                     /locus_tag="BVJ50_RS00065"
                     /old_locus_tag="pMCR_WCHEC1618_00014"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            12082..12432
                     /locus_tag="BVJ50_RS00070"
                     /old_locus_tag="pMCR_WCHEC1618_00015"
     CDS             12082..12432
                     /locus_tag="BVJ50_RS00070"
                     /old_locus_tag="pMCR_WCHEC1618_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            12737..12985
                     /locus_tag="BVJ50_RS00075"
                     /old_locus_tag="pMCR_WCHEC1618_00017"
     CDS             12737..12985
                     /locus_tag="BVJ50_RS00075"
                     /old_locus_tag="pMCR_WCHEC1618_00017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            12982..13329
                     /locus_tag="BVJ50_RS00080"
                     /old_locus_tag="pMCR_WCHEC1618_00018"
     CDS             12982..13329
                     /locus_tag="BVJ50_RS00080"
                     /old_locus_tag="pMCR_WCHEC1618_00018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            13690..14193
                     /gene="taxA"
                     /locus_tag="BVJ50_RS00085"
                     /old_locus_tag="pMCR_WCHEC1618_00019"
     CDS             13690..14193
                     /gene="taxA"
                     /locus_tag="BVJ50_RS00085"
                     /old_locus_tag="pMCR_WCHEC1618_00019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            14197..15426
                     /gene="taxC"
                     /locus_tag="BVJ50_RS00090"
                     /old_locus_tag="pMCR_WCHEC1618_00020"
     CDS             14197..15426
                     /gene="taxC"
                     /locus_tag="BVJ50_RS00090"
                     /old_locus_tag="pMCR_WCHEC1618_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            15503..15796
                     /locus_tag="BVJ50_RS00095"
                     /old_locus_tag="pMCR_WCHEC1618_00021"
     CDS             15503..15796
                     /locus_tag="BVJ50_RS00095"
                     /old_locus_tag="pMCR_WCHEC1618_00021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            15793..16008
                     /locus_tag="BVJ50_RS00100"
                     /old_locus_tag="pMCR_WCHEC1618_00022"
     CDS             15793..16008
                     /locus_tag="BVJ50_RS00100"
                     /old_locus_tag="pMCR_WCHEC1618_00022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            16364..16984
                     /locus_tag="BVJ50_RS00105"
                     /old_locus_tag="pMCR_WCHEC1618_00024"
     CDS             16364..16984
                     /locus_tag="BVJ50_RS00105"
                     /old_locus_tag="pMCR_WCHEC1618_00024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            16998..17324
                     /locus_tag="BVJ50_RS00110"
                     /old_locus_tag="pMCR_WCHEC1618_00025"
     CDS             16998..17324
                     /locus_tag="BVJ50_RS00110"
                     /old_locus_tag="pMCR_WCHEC1618_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            17343..20090
                     /locus_tag="BVJ50_RS00115"
                     /old_locus_tag="pMCR_WCHEC1618_00026"
     CDS             17343..20090
                     /locus_tag="BVJ50_RS00115"
                     /old_locus_tag="pMCR_WCHEC1618_00026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            20102..20818
                     /locus_tag="BVJ50_RS00120"
                     /old_locus_tag="pMCR_WCHEC1618_00027"
     CDS             20102..20818
                     /locus_tag="BVJ50_RS00120"
                     /old_locus_tag="pMCR_WCHEC1618_00027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            20830..21924
                     /locus_tag="BVJ50_RS00125"
                     /old_locus_tag="pMCR_WCHEC1618_00028"
     CDS             20830..21924
                     /locus_tag="BVJ50_RS00125"
                     /old_locus_tag="pMCR_WCHEC1618_00028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            21951..22100
                     /locus_tag="BVJ50_RS00130"
                     /old_locus_tag="pMCR_WCHEC1618_00029"
     CDS             21951..22100
                     /locus_tag="BVJ50_RS00130"
                     /old_locus_tag="pMCR_WCHEC1618_00029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            22439..23125
                     /locus_tag="BVJ50_RS00135"
                     /old_locus_tag="pMCR_WCHEC1618_00030"
     CDS             22439..23125
                     /locus_tag="BVJ50_RS00135"
                     /old_locus_tag="pMCR_WCHEC1618_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            23122..24030
                     /gene="virB9"
                     /locus_tag="BVJ50_RS00140"
                     /old_locus_tag="pMCR_WCHEC1618_00031"
     CDS             23122..24030
                     /gene="virB9"
                     /locus_tag="BVJ50_RS00140"
                     /old_locus_tag="pMCR_WCHEC1618_00031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            24023..25141
                     /gene="virB10"
                     /locus_tag="BVJ50_RS00145"
                     /old_locus_tag="pMCR_WCHEC1618_00032"
     CDS             24023..25141
                     /gene="virB10"
                     /locus_tag="BVJ50_RS00145"
                     /old_locus_tag="pMCR_WCHEC1618_00032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            25143..26171
                     /gene="virB11"
                     /locus_tag="BVJ50_RS00150"
                     /old_locus_tag="pMCR_WCHEC1618_00033"
     CDS             25143..26171
                     /gene="virB11"
                     /locus_tag="BVJ50_RS00150"
                     /old_locus_tag="pMCR_WCHEC1618_00033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            26158..28020
                     /gene="taxB"
                     /locus_tag="BVJ50_RS00155"
                     /old_locus_tag="pMCR_WCHEC1618_00034"
     CDS             26158..28020
                     /gene="taxB"
                     /locus_tag="BVJ50_RS00155"
                     /old_locus_tag="pMCR_WCHEC1618_00034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            28029..28451
                     /locus_tag="BVJ50_RS00160"
                     /old_locus_tag="pMCR_WCHEC1618_00035"
     CDS             28029..28451
                     /locus_tag="BVJ50_RS00160"
                     /old_locus_tag="pMCR_WCHEC1618_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            28435..28740
                     /locus_tag="BVJ50_RS00165"
                     /old_locus_tag="pMCR_WCHEC1618_00036"
     CDS             28435..28740
                     /locus_tag="BVJ50_RS00165"
                     /old_locus_tag="pMCR_WCHEC1618_00036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <28853..29047
                     /locus_tag="BVJ50_RS00170"
                     /pseudo=""
     CDS             <28853..29047
                     /locus_tag="BVJ50_RS00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            29051..29623
                     /locus_tag="BVJ50_RS00175"
                     /old_locus_tag="pMCR_WCHEC1618_00037"
     CDS             29051..29623
                     /locus_tag="BVJ50_RS00175"
                     /old_locus_tag="pMCR_WCHEC1618_00037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            29628..31781
                     /locus_tag="BVJ50_RS00180"
                     /old_locus_tag="pMCR_WCHEC1618_00038"
     CDS             29628..31781
                     /locus_tag="BVJ50_RS00180"
                     /old_locus_tag="pMCR_WCHEC1618_00038"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            31778..31984
                     /gene="hha"
                     /locus_tag="BVJ50_RS00185"
                     /old_locus_tag="pMCR_WCHEC1618_00039"
     CDS             31778..31984
                     /gene="hha"
                     /locus_tag="BVJ50_RS00185"
                     /old_locus_tag="pMCR_WCHEC1618_00039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            32002..32466
                     /locus_tag="BVJ50_RS00190"
                     /old_locus_tag="pMCR_WCHEC1618_00040"
     CDS             32002..32466
                     /locus_tag="BVJ50_RS00190"
                     /old_locus_tag="pMCR_WCHEC1618_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            32553..33257
                     /locus_tag="BVJ50_RS00195"
                     /old_locus_tag="pMCR_WCHEC1618_00041"
     CDS             32553..33257
                     /locus_tag="BVJ50_RS00195"
                     /old_locus_tag="pMCR_WCHEC1618_00041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             3445..5066
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(7334..8205)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          14198..15423
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          20831..21897
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          22440..23122
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          23123..24027
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          26159..28017
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcacacagac atacacatag gtatgtctgt gtgacatacc tatgtacagt gccagatggc
       61 gttgctacgt gaaaaataac ctaccctacc cctgcttttt tcgcagattt tacgtagcac
      121 accgttgcta tagcattcat ctaaaaagct gaatgtcctt ttctgtcgct gttgcatttt
      181 aattcactga aaaatattga gtaatgaaaa tgccggtaac gcgtcaagct gttggccaac
      241 gcgacaggca gtattggaag caacaaaaaa aatgaaagaa aaatccctca aatgaaggag
      301 aaaaaacgga actacagccg aacgacaggc acaaaaaaac ccgactggta atcgggcttt
      361 tttgcgtttt ccagtcttga gttggtggct ctgaccggag aaggttactc tacacctaac
      421 gtttacattt tattcactat ggtgctgtaa agcaactaaa tgtggttaag gaaccacatt
      481 tagtggtgta acgggtgttt tgtgtagcgt ataatcagtc agttagtcat gtagttggct
      541 tgcaatcctg ctgttcccgt atctgcgggt atgaacgatt ttaaagtcct ggctggataa
      601 acaggtggtg ctgtattcgg tgctctggca ggaggaggtc actctacacc taacaatgcg
      661 agagagccgt tgtgacaggc agtgtatgag caagcaacgg tgatttcctc cagttttact
      721 ggagaaacgg atgattaata tgttaatcat cgttctcaga gctgtggtcg ccgttgcaaa
      781 cgcgctgatt gcagttctgg aactgatccg ccaattcatc gattgatgac aacggaaacg
      841 taactaaggg caggaagtga accgcttcct gccctttaac aaaaaaggag gatttatgga
      901 ttttatgagc attttggggc gctgtttact tcttcctttc cctgttgcga tgtttgtcgg
      961 ttgcctgttt cctggcatcg atgatcgctt atccggaatg cttatgagtg ttgctgtggg
     1021 atgtttatcg tggtatgtca ccagaccgaa gaaccgacag acaaaggccg cgtaagtggc
     1081 ctttaattgg gatacccttt agttacaaca ttctaatagc tcattccatc ctccgctcat
     1141 ccagcaaaca taacctccct gcccctcccc actacagaaa gtggcgcgcc ccgcccgtac
     1201 gggcggaatc taatcgctta attttgaatg ttgtaactaa acatactcat tgctgtattg
     1261 ccacaaggtt tcgcagcaag ggaggatacc gaaagtaacg ctgtcacttt tgctttcaag
     1321 tgaccaacca acgggtacat cacactgaca tacaataatg tatttcttca tgatgtacat
     1381 cattgtaatt cattgtgaca tacactatag tatgtccatg tgacatacgc atgcgtacat
     1441 cacatagaaa tacacatgag taaatcactg tgacatacaa ggggtactat gattatcgta
     1501 atcggtggtg ataaaggcgg tacaggtaaa tcacatctgg caaccaactt aactgtttgc
     1561 ctctcacaac agggaaagag aacggggctt gttgaaacag acctgaatgg ctcgaccaaa
     1621 aaatggaata aacgcagaga acaggccgga ttaccgcccg tcgcattaaa tgaagcctat
     1681 ggcgacatca gtgccaaaat aacaaaaatg gcagatgtag cagaaatcct cattatagat
     1741 actgccggat atgacagcac ggagttcaga actgcactaa aagttgcaga cattgtgatt
     1801 gtcccgatcg acccgcttgc ccaggtggaa gcagacagtc ttcagactgt gacaaaaatt
     1861 gtcagggatg cgcaaaaaat aaatcccagg ctggctgcac atgtgctcct gtataaatgc
     1921 cagccaaata cgttcagtga acaacaagaa ttaagagact ctctcaacag ccatgattac
     1981 tggcttaaac caatgaaaag cacggtttct tttctccggg cctttgtaag agcaatgaat
     2041 cagggaatgg gggttcacga gttaaaaagt aatgttagcg gtgccagcca ggcaaaagca
     2101 caaatcgaac tcttgctgaa agaactggaa ctgtaaatca tagtgacata cactaatgta
     2161 catcatcatg atatacatta gtgtgaaaca atcagattag aaggtacatc atggcaaagc
     2221 tggaactacc agaactagaa caaaaccagg aagtgaacgc cacaaaattt gttcaggacg
     2281 caggaaaacg ccccacagag aacaaaacaa aactggcttc attccgcata ccgaatgaat
     2341 tgcttgaatt cattgataac gaatcaaaaa gactgcatcg tcccaagaca aaaataatca
     2401 aagcagcatt actggcttat caggatctgg acgaaaacga actcattaat ttgtggttca
     2461 gagctgacag aaatgattaa tcatagcaag tggcccaata aactctttat cattttcttt
     2521 tagatactta tctgaatgta ctaacttcat cacttttaaa tgtagttgtg attcattttt
     2581 agttactctt gcaataaaat acccttgtac agttgttgca cgggtattta ggagcactag
     2641 atgagcctta tcgatttaag tttatcagga ttatcagaac caggaacaaa acttattgaa
     2701 aaaataagtg atgctattgg tgtgctttat gaacctacaa gaatcaggaa aaaagcgaag
     2761 gctgaagctg aagctaaacg cacagagcta atttctaggt tagagcttga gggaatagaa
     2821 aaacgggcag ttgagcgttt tctcaaacgt gaaacgaaac gacaggaaaa cattgaaaat
     2881 ataacaatgc aagcagcaca aagtctatct gagagtgata atgtttctga tattgacgaa
     2941 gattggatag aggctttctt cagggagtgt gaggatatta gtgacgaaca aatgcaaatg
     3001 ctttggggaa gaattttatc cgaagaagca aagtcaaaag gttcgtttag tcgtagaacc
     3061 ttaaaactat tatccactat aagcaaagaa gaagcgaacc ttattactta ttttggaaag
     3121 tttgtttggc aggcaaacaa actcactcca attttattta ccgatgagaa tggagatact
     3181 gaaggtataa ctttcgataa actttcggtt ttagactcct taggcgttat tcaacaaggc
     3241 attggttata gtttaagata caaattataa atactctcaa gtgtatattc agtatgggat
     3301 tgcgcaatga ttgcctaata aaatttctga aatatttctg tatcgcataa ttttttatat
     3361 cagataaatt gtactggatt tcttaaaaaa ttgcagtatg attgccgcaa ttatcccacc
     3421 gtttattttt tgagtagttt ctcatgatgc agcatacttc tgtgtggtac cgacgctcgg
     3481 tcagtccgtt tgttcttgtg gcgagtgttg ccgttttctt gaccgcgacc gccaatctta
     3541 ccttttttga taaaatcagc caaacctatc ccatcgcgga caatctcggc tttgtgctga
     3601 cgatcgctgt cgtgctcttt ggcgcgatgc tactgatcac cacgctgtta tcatcgtatc
     3661 gctatgtgct aaagcctgtg ttgattttgc tattaatcat gggcgcggtg accagttatt
     3721 ttactgacac ttatggcacg gtctatgata cgaccatgct ccaaaatgcc ctacagaccg
     3781 accaagccga gaccaaggat ctattaaacg cagcgtttat catgcgtatc attggtttgg
     3841 gtgtgctacc aagtttgctt gtggcttttg ttaaggtgga ttatccgact tggggcaagg
     3901 gtttgatgcg ccgattgggc ttgatcgtgg caagtcttgc gctgatttta ctgcctgtgg
     3961 tggcgttcag cagtcattat gccagtttct ttcgcgtgca taagccgctg cgtagctatg
     4021 tcaatccgat catgccaatc tactcggtgg gtaagcttgc cagtattgag tataaaaaag
     4081 ccagtgcgcc aaaagatacc atttatcacg ccaaagacgc ggtacaagca accaagcctg
     4141 atatgcgtaa gccacgccta gtggtgttcg tcgtcggtga gacggcacgc gccgatcatg
     4201 tcagcttcaa tggctatgag cgcgatactt tcccacagct tgccaagatc gatggcgtga
     4261 ccaattttag caatgtcaca tcgtgcggca catcgacggc gtattctgtg ccgtgtatgt
     4321 tcagctatct gggcgcggat gagtatgatg tcgataccgc caaataccaa gaaaatgtgc
     4381 tggatacgct ggatcgcttg ggcgtaagta tcttgtggcg tgataataat tcggactcaa
     4441 aaggcgtgat ggataagctg ccaaaagcgc aatttgccga ttataaatcc gcgaccaaca
     4501 acgccatctg caacaccaat ccttataacg aatgccgcga tgtcggtatg ctcgttggct
     4561 tagatgactt tgtcgctgcc aataacggca aagatatgct gatcatgctg caccaaatgg
     4621 gcaatcacgg gcctgcgtat tttaagcgat atgatgaaaa gtttgccaaa ttcacgccag
     4681 tgtgtgaagg taatgagctt gccaagtgcg aacatcagtc cttgatcaat gcttatgaca
     4741 atgccttgct tgccaccgat gatttcatcg ctcaaagtat ccagtggctg cagacgcaca
     4801 gcaatgccta tgatgtctca atgctgtatg tcagcgatca tggcgaaagt ctgggtgaga
     4861 acggtgtcta tctacatggt atgccaaatg cctttgcacc aaaagaacag cgcagtgtgc
     4921 ctgcattttt ctggacggat aagcaaactg gcatcacgcc aatggcaacc gataccgtcc
     4981 tgacccatga cgcgatcacg ccgacattat taaagctgtt tgatgtcacc gcggacaaag
     5041 tcaaagaccg caccgcattc atccgctgat ttctccctgt attttttcca aacccaccgc
     5101 acactccatt cgtattatgg gcggtggggt ggggtttgtt atgccgtatt tatcaaataa
     5161 acgcctactt gctgagatga gtatcgctct tgtcatggcg atcgttgcca cgctgaccct
     5221 tgagcacagt cagattgatc tgatggtcgc tgattggttt tatctgggta tggggcattg
     5281 gatggttgcc aagcaagctt ttttgccaga tttgctactg tattctggac taaaaaagct
     5341 gctgatggcg atgctgatct acttgctggt tgcgaccatt tgccgtgctt atcatgagaa
     5401 aaagggcaat gctatcactg ccaagtggct tgtcccagtg acaaaatttc gcgtgcgtga
     5461 gcttgcgtat ctggtgctga ctttgatcct agtgccgaca gttgtcgcgt cattgaaggc
     5521 atatactcat gtggtctgcc ctgtgcattt gacgattttt gatggtacgc tgccgtattt
     5581 gccgatgctt gatagtatgc gtaacaccat tcctgataag tgctttcctg cggcgcatgc
     5641 cagtagcgga tttgcgctgt ttgcctttgc gtttgcgcca agtttgcgcc gccgtcgtgg
     5701 tgcgatcatc atcgtggtga tggcattggg ctgggcgatg ggctgctata agatgattat
     5761 tggcgatcat tttttgagcc atacggtggt gtcgatgatg cttgcgtggg cgatgtcggc
     5821 agggcttgcg tgggtgtttt ttaagaaggg tgaacaagtt taatcggctt taaaagtaaa
     5881 aacggtcgta tcggttatta cggaacggtt ttttcagttg agtttcaaag cgatgattca
     5941 tcaacctgga aactaccaat aggaacggct ttacttacgc ctatcggtca agaattgatg
     6001 aaaatatgcg gttccactcc agatattgcg tatttaaata aattcctcaa caaaataaat
     6061 gttgaaggaa gccaggtaaa actatcaatt attaatatgt aaagaggtcg agttacctgt
     6121 ctgagttcca ctaaccataa ttaaaagcga ccgcaaaaac tgcccagtga aaactgtgtt
     6181 gccagtaatc acaaatgcag caagtactaa taaagagcct gttaagcccg caaatatgtg
     6241 ggcttatttt taattactct tcatcatccc gcaggaactg acaaaaatct tcgctcccca
     6301 tcatatcgac agtgttttta aattcgaaaa aagaaattcc tgtcatagca cacacgcgct
     6361 ggataatcag ttttttcttt tgctgtctga cggttgcggt ttcaccaaac tggataacag
     6421 ccaggactgt ttcatcatat gatttcagga caaaatcaca ggttatattc tgcacttcac
     6481 ggattaaatc ttttacagct tcctcgctac catgaggacg aacaaattca atgagccgta
     6541 ttttgggaaa aacggaataa tcaggtataa tattacgtga aagaatgcct aaaaactctg
     6601 attcattatt tgagagtaag ggtactttag taaaacaatc aacctcccca atggctgtaa
     6661 aagcattggt tgtcaaagct ggattgttct taataaaatc agagaataca ttttcagctg
     6721 atggcaaaac aacagcagaa ttatgttgtt catctcgttt tttattacgg ttaaatatca
     6781 tttcatattc cttttaaaag agcctgcggc aggcacaggc aaaaacatcg tttttggaca
     6841 atctgattca aatgttaaca tttgacatcg gattgtctat cctgcattgt tctttactgt
     6901 ctgtaagatc ggcgctcatt cttccggcgt tctcccgata ccaaaaagac ggaacgcaaa
     6961 acggctttcg atccaccatt caaaaatctg ctctgcgcaa ccacaaacag ttaaggcgat
     7021 ccatgcagca agaacaacgt aactactgac atctgcaccc atgtaccagg cgatagttaa
     7081 acccatcact gcgataccgc ctaaaaactg caacaagaaa cgaaatcccg caagaatccc
     7141 caatggaata acaacaacca gataaaggga agcagccaga aggtatctga caggtgtcag
     7201 cagataaaag aacctggata ttttcttttt gtcagaataa acggcatcat ttgcaacatc
     7261 ggttgaatga tcattcatgc tgtccctgat tctttgaaaa tctacgatat tatccttcat
     7321 attgttatga cctcaatttt caggtgggta cattttatca aacatattag taaactctga
     7381 gtcttccctg gtatcgccct cataaccaat tgtataagag aactttaatt tataaacttt
     7441 tctcccaatt ttttctgaaa ctacgaaact taagttcttt acttccgtat gtttcatgat
     7501 ttcttttact gatttattaa gaacatctct tttaaaagca ggataatctg gatacttata
     7561 atccttcacc ccccccgccc ctattgtata aagattcaat tcatctttaa gttcatctat
     7621 tgtcatttca aaactattcg cgcgagaatt attactatat attttacgaa tgagttgata
     7681 tagtgatgca gcattaacac ttgtcagtcg aacaacagat aataaaactt gcgttgtata
     7741 acgtttttct tcaccaataa gtttacataa aaatcttttc gcttctttcg taaacttgat
     7801 ccccactcta ccttcattcc tgtaataagt agaaaaaaca gtcagactca aattcattga
     7861 atcaggtaca ttacttttag tgaacttaaa tcccaaatca ctactcagtt ctaatatttc
     7921 atcatgtttc agcttaagaa gcgtcgtatc taatatatta gagccatccc tcaaagcaag
     7981 ataagccgca tcaggcttaa cctgaaccca tttaatgtaa tcggcaactg tcacataaaa
     8041 tatgtgatca tcatcgaatt catttttaga gtttatctgg cacatggcta aaaataagac
     8101 ttttttagca gccataggca atgttgacag cgttgaattc aattcatttc gatgtctcac
     8161 ttttgttaag cgagaaaggg aagtcttatt tgtcgtcatt ctcattgttc caacactctt
     8221 gataatcaga ctttaacacc agaaacatga tattacaaca caatcaatat catgtttaac
     8281 cgagtatctt ctcatgtttc atatctgtga tttatctcac aaaggtaatc catgctcaaa
     8341 atccacactg gattcaaggc agattaacaa cagattcaaa atagattcac cagaaagaac
     8401 aacgaattag tattcttatg acacattttg tggatatcac cgaggaagct ctcatgttta
     8461 accgaggaag ctctcatgtt taaccgagga agctctcatg tttaaccgag gaagctctca
     8521 tgtttaaccg aggaagttat catgtttaac cgaggaagct ctcatgttta gacaaaaaac
     8581 acgcttaaaa tcaataaatt agagctactc taagttttta agtttttaag aaaaacaaga
     8641 aaagataagg cttttaatgc gtgcgaatat tcaggaactg tggataactc aacaaaacca
     8701 gcaacgatga tggttcctta ccataaatct gtcccctgat gattattacc cgcaagctaa
     8761 aacaaacatc atcaatcatt aaaactcata actttcaata acataaaatc atcaaaaaaa
     8821 tatttgatgc cttatgtatc tcaattttca agaccataaa tttgtcccta gttaacttta
     8881 acttattgaa ataaatataa tattcgtgcg tactgatcct aatgtcacat cgcaaaaatt
     8941 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggtatct
     9001 cctttctcaa aaccagagag attcagatca tatgatccgt tatttgatcc tttttggatc
     9061 ttccactaag aagccctgaa tcgcgctctg ccgcttcgtt cgatacaacc atgaggaaaa
     9121 ccatgcgaaa tgaaaaaaat cgcttaaaat gcgattcaga gcgttttagg gaggttctgt
     9181 tagttctcct gcattgcgcc cgtagttcgc taacgctcac acgcgcagta cagcgccccg
     9241 ccatgctgca atgcaccagg gcggggtaaa ctcgcttgcg ctcgcgctaa ctgcggcatt
     9301 ctgcggcgca aatcagcata atcatggcct gctggtcagt tgggctttgc agggtgaagg
     9361 gatggcctgt gcaccctggt acgttcgtca gcggctgcgc cgcgcttcgg ggcgatgtgg
     9421 ctcctgtgct gccctggtat tttcgttcgc cccttccatc cgcttacgcg gattaaaaaa
     9481 cgtgataaca atcacttatc gagtaaaaaa acgggagaaa acttgcaaat acgacttaaa
     9541 ggtcgtatta ttgtttcatc gaaaggggaa tggcccctct cacaatagcc cagcagggca
     9601 ggagattaaa aaaatgaata tccaggaagc attaaacgtt tttggattat ccggcgaatt
     9661 aactgaaaaa gatatcaaag cagcatacag aaaagccgct ttaaaatatc atccagatcg
     9721 taacccgtta ggggctgaac tgatgaaagc ggtaaatgca gcttttgatg tattgatggc
     9781 aaatattgat aaaataaatc agttccagag cactgatgaa catgcacgat ataattacgg
     9841 tgatgacctg gaaaaagtat taaacgttct ttctggttta tctggtctgg tatttgaagt
     9901 gataggtaac tgggtctgga ttagtggaga aaccattaca cataaggaaa ctttaaaaga
     9961 aatcgggtgt aaatgggcgg caaagaaaaa acaatggttt tatcgtccag acgaacataa
    10021 aagttactgg aatcgtgaag aacacacgat agaagaaatc cgcgcaaaat acggtacaac
    10081 cggacagcgc agggcgacag ggtggcaacg cgtggaaacc agagcgtaac cagaacgggg
    10141 gcgagaagcc cccgatatta tagccagccc gtcttttggg gcatcgaacc caaaaagacg
    10201 ggaacagcag ccacgtagca aaaagggaaa cagatgaact ctttttttga acaatatcac
    10261 cctgtctttg aagttgtctg ccgtatcctg gggaacggct ggcgcgtgaa caaacttgat
    10321 gattgttcgt ctcgtataaa actgacgtca ccgcagttta aaaattactc tgtgcatatt
    10381 cggatggaaa aagaccgctt ttctgtggtg gggagtgtgg atagtcgttc ctggcgtagt
    10441 ccgcatcatg tttgcacgtt atccagaaaa cggaatcccg ttgatattgc agccgatatc
    10501 gagcgaaaaa ttctggtaaa tgcttcgcag gaggtgttac aggcgattga gtatgagaaa
    10561 caccaggtgg aaaaaaagga cgaaattctg atcctgaaag gtatgttatc gcaacttgtc
    10621 cagcttgaaa gctggtatgg ggcattgaca ggctttaaag ctgaaaacgg attgaacggt
    10681 aaagtcaccg aacagggcga cagttatgat ttacagattc ggggcttgag tatagatcaa
    10741 cttgttaaaa ttacagggta tttgaaacag ttatgagaaa aaataaaggt gatgtgactt
    10801 attttcttga aaaagaaggt gacaattaca ggcttacaaa aaggataaaa gccagaacaa
    10861 atgttaaaat cgggaacaaa actacgaaaa ttacattgta tgatgctgta ctgaatgaga
    10921 atgaattaca acatattgat tttacgtgtg ccggattaag ggaagacgat gagacgccag
    10981 ttaagaattt aattaaggag tttatgttaa atgaaacccg ataatacacc tgaaaatgta
    11041 aaaaaattac gtttaaaagc aggattaaca caaaaggaat gcagtcatat ttatggtgtt
    11101 gggctaagaa cctggcagaa aaaagaggaa gttaatactc aaaacagtca aagtctgtca
    11161 ctggttgagt ttgagtttct tttgttactt gcaggagaac atcctgaata cgtgctctgc
    11221 aaaagggagt caaaataaaa tgacctgtga tggttttata ttagtacctg gagttgcgct
    11281 cgtagctcgc tgacgctcac acgcgcagta cagcgccccg ccatgctgca atgcaccagg
    11341 gcggggtaaa ctcgcttgcg ctcgcgctaa ctgcggcatt ctgcggcgca aatcagcata
    11401 atcatggcct gctggtcagt tgggctttgc agggtgaagg gatggcctgt gcaccctggt
    11461 acgttcgtca gcggctgcgc cgcgcttcgg ggcgatgtgg ctcctgtgct gccctggtat
    11521 tttcgttcgc cccttccatc cgcttacgcg gattaaaaaa cgtgataaca atcacttatc
    11581 gagtaaaaaa acgggagaaa acttgcaaat acgacttaaa ggtcgtatta ttgtttcatc
    11641 gaaaggggaa tggcccctct cacaatagcc cagcagggca ggagatttaa aatgaaatgc
    11701 tcatctgttt ttacttcaac cactaatcat gttttcacat tcgaacgagt aacgctttgc
    11761 actattatcc ttatgcataa ggatactgga caacagtatg ttgtcatatt tactgataac
    11821 aataaaattc gtgactataa agccggaatt gttcctcagt ttggcgaact taagcagagt
    11881 gatgttgatt tagtcctttt ctacagggac gaatatgaaa aatattttga ttcattaaaa
    11941 gatggcgatg aatgcttgag ttttaaagat tttattgagt gtctttgtta agcattatga
    12001 atgcaggggc atccctgcat tttctggaaa tttcaatatt gattctgaaa ttgtctatta
    12061 acatcaatgc aagggaaatt catgaaaacg ttaaaacaag ccgcaatgca gtttgcatct
    12121 gatctgcgta ataatcgctg ttttaaagct gcttataatg atgctctgtt agagcttgat
    12181 cgggaagatt taaacgccat tgtacaatgc acatttcttt ttgagccaaa acgaacatta
    12241 gccgaacttg ataaattaat taaaaagata gaagtagaag aagattatga gttttgtgta
    12301 tcccttgata aaggtaaagg attacacgaa gtcagaacat ttaaaagtga gcgtgaggcg
    12361 ttggatgctt atcgcatttt gactctttac ggatacacag taactataga aaagaaggag
    12421 ggtaatgact gactattcaa ggtgtgatca ggttgttcca ccaacctgat cacgttatcg
    12481 acaatcaaaa ctgaatgagg tttttaaatg tcaacgcggc agatcgtaca gcttgctgat
    12541 tcccttgtca aaggctggga aaccagaatt ccggcaatga aatttagcga atgggatcaa
    12601 ttccggtggt ggttaaacta cttgcaaggg tatgaaatgt tttaaataaa aaaaggggct
    12661 ttctgcccct ttgtattttt tgacaaaaat gccatgtgta actataatta gttgcatatc
    12721 tataggggat ggatttatgg ggaaaacaga taagctactg gcaaagtttt taaacagtaa
    12781 aaaaacgttt gaatgggatg aactggtcgt tttgttttcc tctttgggat atgtcaaaaa
    12841 ggaaatgcag gggtcaagag tgcggttttt caatgctgaa atcaatcaca ccatattaat
    12901 gcatcgtcca catccggaaa gttatatcaa aggtggtacg ctgaaagcaa ttaaacagaa
    12961 tctgaaagag gctggattat tatgaaacat ttaaaatata aaggatattt aggcacggtt
    13021 gaacccgatt ttgaaaataa tgtcctgtat ggaaaactgg cgtttattcg ggacctggta
    13081 acttatgagg cttctacatt agctgaactg gaacaggagt ttaaaacatc tgttgatctg
    13141 tatttacagt cttgtgtgga ggatggaaag gaacctgata ccccgtttaa aggcgtgttt
    13201 aacgtcaggc ttgatccaga actgcatcgt cgggtagcag aaatggcgat ggaagaagat
    13261 ttatcgttga atgcctttgt taataaggct ctggaaaaag aagtcagcaa tcatcgcgca
    13321 ggggcttaat tgcccttgtt tttatgccct gtaagggcat gggaggcgct ttgcgccgga
    13381 agccccgcag cgcagcgagg acttaaaaag cgttacggct tcagccgtaa caattcccag
    13441 ccctgccagg cctgtttttt ctttcacaaa tctttcagct atacgtttgc ctgatggtcg
    13501 aagacgaaag aatctttcag caacgaaatc tggacatatg cagcgacgat tttatagcca
    13561 ggcacagtgc cacatatggt acaatcaatg caggatagac aatccgatgt caaatgttaa
    13621 catttgaatc agattgtcca aaaacgatgt ttttgcctgt gcctgccgca ggctctgttg
    13681 gggtgactta tgaagatgat agcgttcagg ctaagtgatg atgagttaaa atttgcagaa
    13741 cataacgcta ttttaagtgg atttaccagt atcaatgctt ttgctaagca taacgtgctt
    13801 aatattgaaa ccaagcctgt taacatccca gttaataatg aaccagcaaa gctggtatca
    13861 gtgcgtcttt accctcatga aatagaactt gttaaacgta atgctgctct tcacggcatg
    13921 agcatgagca gagaaattgc tattcgtgtg cgtcagtctt tgttaaagag tgaagtctgt
    13981 ctttaccctg atgaagttaa ggaacttaaa aaactatcaa ctgctgtaga tcgtgttggt
    14041 cgcaacattc attttatcat aaaaggtgaa agattctgta cggttaacga tccagatttc
    14101 agaaaagacg ttattgaagt gattgaactg tgtaagcaaa ttgattcaaa acttgaaacg
    14161 ttaaccaaga gtgttgttaa cagatttggg tgatttatgg gtgtgtatgt agaacaggaa
    14221 taccgtatta aacgcgctaa agggactggg cgtgatccga aatctcctaa gttatcggga
    14281 agacatatac atgccagtaa atcggctttt aatcataaag tcagacatgg tgcggacaag
    14341 aaagcatata cctggacacc tacaaaagaa gtcactttta aaatcactgg ctcaggtaaa
    14401 acagccgcag gcatcaaaaa tggtattgat tacattaccc gtaatggtga acttgaggct
    14461 tactgttatg atggaaaagg ttcagagcaa acagggaaag gagaagactt taatagggag
    14521 tttacttcaa ctttatctaa aggaaatgat tactccagga catatcgtgg agaaaacatc
    14581 gatcatgtga aaaacatggt cttttcacca ccaccagaag ctggagtaag cagggaagac
    14641 gctctgaaag ctacggtaga atttcttaaa gagacttatc caaaccatgc tttcgttgcc
    14701 gtataccatg atgataaaga agatcatcct catgtacatg ttaatattaa acttcgtgat
    14761 gaagaaacag gaagaagatt aagacttact aaatctgaaa ccaggaagtt cagaaacggt
    14821 tttcatagaa aactgaaagg tatgggatat gatgttaccg ctacctggaa aaaagatccc
    14881 gaacgtaaac gggagattga gcgattacag gctgaaaatc cgaaaagact ccggaatgtt
    14941 tacaaggtgg tggattttgg ggagacttca tatcagaata aggcagggga aaaacgaaca
    15001 cctttcctca cttatgaaac tttaaaaggt ggaaaacagg ttacgatttg gggtaaagac
    15061 ctgaaaaatc atttcgagtc tgaaaagctc cagccaggaa cgcttatcaa agtaaaaaaa
    15121 ctggctccta cgcttgtacg tagtccaatg tttaatgatg atggaactgt tgcaggctac
    15181 agggaaacgc atcgcaataa ctggcagatt gaaaatattg cccttgagcg gaacagagaa
    15241 cgtcaggttc atgagcgtga aaccaggcag ccggaagaga gcgacgttaa aaagcagcta
    15301 tccagaaaac atgagcaagg tcacaatatc gggtttgctc ttgaacacgg gtttacaaag
    15361 gattcagaag aacataaaaa actaagaata cagcaagaga ggaactggaa aggcctggga
    15421 ttttaatttg attttggcgc aggtgaacaa agcggctact ttgctcacct gctgatcaca
    15481 acaacctacg agggaggtta tcatgactga gcatgatgct atctgcattt ctggacttca
    15541 tcaaattttt agtgatgaag aacatttgag tgagcaacaa aaagacatta ttctgatgta
    15601 cgcttatggc tataccctga atgaaattgc tgatttcaaa ggactaaagc cttcaactgt
    15661 cagaaaatat ctcgattctg taagagctga actgggaggt gtctcgttgg cagggatcag
    15721 aacactggta ctcattcgga ctaatgccct tctcgtgtca agtctttcca ggatcagtga
    15781 gagggggaat ttatgaaaaa atatctgctt tttgctttgc cgttcttcgt tgttggttgt
    15841 agtgaagaag taaaaagtgt ggattggtgg gggcagcatc ttacggaagc taaacaaaag
    15901 caggcagagt gtgagaagtc tggctctgat tctcagaact gtaagaatgt taaacaagca
    15961 ttgtttattc agtctcagaa ggatgctcca gtccctacat tcgattaact aatgtttcaa
    16021 tcatactaga gcgcccatat gggcgctttt ttttgcatga tcacacgaat cataatatga
    16081 ccattttgtc aatgttattt gatcgtattg ttgagcattg ttattttgcg aatcatgttc
    16141 gcatctaaaa caaatccaaa acacacttat agtggattga ttaaatgaag gagaaatcag
    16201 ataagtcatc ggaaaaaggg gtttatgtca cgataaaact cgatcccagg ttgaatgcat
    16261 ttatcgaaga gaaaacgaaa acatctttgc ttagtaagag gaagcaaatt atttacttat
    16321 taatgcagag tatgcgtatt gatggatata tggagcgtta gctatggcgt ctttattccc
    16381 agccttgttg ctgtgtgcgg cgtcagttca tccggatacg ataaatgata ttgcacgggt
    16441 cgagagtggc ttcaatcctt atgctgtggc tgaaattatt cctgaaaggg aacgcggtag
    16501 ctatggcaaa agtgttatca gtcatatgcc caaaagtaag gaggaagctc taacagttat
    16561 cagagaaatt gaaaacagaa aaagacgtta ttctgtcgga ttgatgcaga ttaccagcag
    16621 taatttttct ttttattcca cgtccgcaga aaaactcctt gattcgtgtg aaaacttgtc
    16681 tgttttcgaa aagattatcg tggattgtta taaacgaggg aggagcctgg agaatgcgct
    16741 tagttgttac tatacaggaa attttagtaa cggtaagcga aaagaaaaag aatttaataa
    16801 tacaagttat gttgagcgaa ttggttatac agggaacgaa aaaaaatatg ttgtaccagg
    16861 tacaagaagc aacgggggag aacagcgaaa aaatcgttca cacaatgcgt cagttatctg
    16921 gcccgaaacc attctgaaat ctgcttttgt tgataactca catccaacaa aagttattaa
    16981 ttaacaggac ttataaaatg tcatttaaga aatcagtatg taatcgtatc agttcatctg
    17041 tcactgctgt aatgcagcgt aaaaataagg tagttatggc agtcgccggg gcaacagttg
    17101 catcaccggc ctttgcggat ggattttcaa aagcggaaac actgctggaa aaggttaaaa
    17161 cagggcttag tggcctttca ctggtcacgg tgactattgc ctgtctgtgg gtcggctaca
    17221 aggttctgtt tggtggaagc acgattcgtg aatgctcacc tatcatcatc ggggcaattg
    17281 ttatcgccag tgcggctgaa gttgcatcaa tgatggttaa ctaataaagc ggggacgata
    17341 gtatgtctac actttataaa gcgatgacac gccctgctat gtatgtgggt gttcctgtcg
    17401 tccctcttac tgtggttgca ggggcgttat ttctggcggg ggtttatatc agcaagctga
    17461 tctggctggc tattcctgtc gcggtttttg ttctcagaat gatcactaaa caggacgacc
    17521 acatttttaa cctttacttt cttaagctga aaatgctcgg aaactcagtg tgtaatcgtt
    17581 tttttggcgc acgagcgttc ctctccggac aatatgaagc tgttgaaatt gatgagtttg
    17641 ttaatgccat gaaactgaat gagcgaatca ctactggtaa atatataccc tattcaagcc
    17701 atgttgataa aaatattgtc aaaacaaaga acggtgatta tgtagcgaca tggcaactga
    17761 tggggattaa ctttgagtca atctctgcgg agatgttaga aactattgac tcacaggttg
    17821 ccactcttgt tcgttcattt tccggactgc ccgtttcatt ttataaccat tcatgcaggg
    17881 cgagttttta tgatgccttt accacaaaat caggtaataa atatgctgat ataatatcag
    17941 attgttacta tggttcgatg aagaaaaata aattcaaggg gaacactctt tactttacgc
    18001 tgatctacag accagatggg cgtgtagaaa aactggaaaa acgcaaaaaa agtattaaag
    18061 aaaagaaaga tgatattaac attcatgtga agagaatgaa tgaaatgatt aatacattct
    18121 ctggcgctct tgataaattt acctgtaaac ttcttggtat gtatgaggag aacggaaagg
    18181 tattttcttc tcaactgtca ttctataatt atcttctgac agggaagttg caaaaaatca
    18241 gagtcactga ttctccggtg tataacgttc ttggtggagt ggatgtattt tttaatcatg
    18301 ataccggaca gatatgccgc attgatggaa ataaattctt tcgttctatt gaaattaaag
    18361 atttctgtag tgagactgca tccggtgttt ttgatgtgct ccagtattct gatgctgact
    18421 atattattac gcattcatat acatccatga gcaaatcaga agcattgagt accattaaac
    18481 gtgcggaaaa acagttaaag agtacagaag atgatgcggt tactcaattg caggaactgg
    18541 aaaaggcaaa aaatgatatt gtgtccggtg atatatcctt tggttactac catttcacgc
    18601 taatggtaat ggcggacagt atcagggagc ttgacgaatc tgtaagtaaa attacggcag
    18661 actttactga cctgggcatt attccggcat tgtcaaccat gtcattgcca gcggcatatt
    18721 ttgcccaact tccggcggtt tttcatttaa ggccgcgttt atctccggta tcgaatgtaa
    18781 attttgttga actggcatca ttccataatt tctaccaggg gaaacgcgat aaaaactgct
    18841 ggacagaagc ggttgctatt ctcaaaacac caagcaaaca ggcgtattat ctgaatttac
    18901 ataactcagt actgtttaaa gatgaaacag gtgaaaagaa tcttgctaat actaaagtta
    18961 taggaacggc gggttcaggt aagacaatgt ttctgtcata tcttgcctgt tcattacaga
    19021 aatacaataa ccccgaaact tttgctgatt ctgcaaaaaa taagaagtta acgtgtgtct
    19081 ttcttgataa ggacagaggg gctgaattat gtatccgaat gcttggtggg gaatattaca
    19141 ccgtaaaaag cggtgagccg acaggatgga atccttttgc tctggaggca acaaaacgta
    19201 accgtatttt tgtcaagcaa ctgatggaga ttctgtgtac cagaaatggt gaaagattat
    19261 cgacaagaga aagattgctt attagcgaaa gtgttgatgc tgtgatggat tttcctcctg
    19321 gcgaaatgcg ggaatatggc attaccagaa tgcttgagca tcttatgcag agagatgatc
    19381 gggatgagca ggagaacggg attatccttc gtttgtccca atgggctaat ggtcaggctc
    19441 atggctgggt ttttgataat gctaaagaca cattcaatat tcagcatgtc aataattttg
    19501 gcattgatgg caccgagttt cttgatgatc cgatggtatg tgcaccaatt actttctatc
    19561 tgctgtatcg cattacgcag cttctggacg gtcggcgtct tgttatcttc ctggatgagt
    19621 tctggaaatg gctacaggat gaagcattca gcgactttgt ctataacaag ctgaaaacaa
    19681 ttcgtaagct gaacgggctg gttattccgg caactcagtc tccggatgaa atactgaaga
    19741 ataagatttc acgggctgtg gttgaagtat gcagcaccag tatttatctg gctaaccctg
    19801 atgccgatta caatgattat gtggaagggc ttaagctgac accagaagaa tttaatattg
    19861 ttaaaaatct tgatccaatg tcgagacagt tcctgattaa aaaaagcagt cttaaaaagg
    19921 gggatggtaa atcattttca gcacttgcga cactcgattt atcgggactt ggtggatatc
    19981 tgaaaatatt gtctgccagt gcagataatc tggaaatttt tgaaagtatt tatcatgaag
    20041 gaatggagcc tgatgactgg gttcctgaat atctcgaacg ggcaatctga caggtgaatt
    20101 tatgaaacgt gtaaaaacat tgatgctgat atcattattg acggcttcat tttatagtag
    20161 cgcaggaatt ccggttgcta ttgatgccag tccggaatgg gctgttgaag ccgcaagatg
    20221 gacagaacga ctcaaacaat ggtccgaaac tgcgcaacat tatcagagtc agattcaggc
    20281 atataaagac cagttagcca cggcaacagg tataaggaat attgcggcat tcacaaatga
    20341 actaagtaat ctacaatcag aactgacaaa tatttataag caggggaaca gttatatttc
    20401 tgattttacc agtaatcccg aaggtgcatt gagcagccag gctaaaaatc tgttcagcaa
    20461 gtatggagcc ttcgatatgt gtaatacggg ctatgaacgt aatgataatc tctgtaaagc
    20521 acggattgtg tcaacggctg ccagtattga acagggaaat gagatcaata agcagttatc
    20581 aagtgcaatg tctcagattc agagtttaag tagccgaatt gaggcttcaa aggatatcaa
    20641 agaatcccag gatttggcta acgcattaca ggcgcagtcg ttaaaaatgc aggcgataaa
    20701 aatgcagtat gatgtctgga ataataaaaa taaagcagat catgagatgt tggtcacaca
    20761 agaacaagag gcctttatta aacagcagaa agaagctgaa ccattaactt ttgattaaaa
    20821 ggtggatata tggctcaggg gtttttcgtt aaatataatt ctaccgttat ggattcggta
    20881 gataaaatca gctccagtta tcaaacacaa tttgctaatg atattatgtc gttagcaacg
    20941 gtatcggtta cgttatatgt actatggaaa ggatatcaaa tacttgccag taaaactcag
    21001 acgcccttgc aggatttagt atgggatcta tcaaaatttg caatcataat catgtttata
    21061 actaatgcgg atgggtattt aactgcggct actgatgcgt tgcaaggcat gaaagatggt
    21121 ttttctggag gggtgagtgt ctggcaaact ttagataact tatggaaaag tacgcaaaat
    21181 cttggtgcgg aaatatactc tttagataaa tcaacatatg ttaaagatca aggtgtagtt
    21241 ggtcagtttt taatatggac tggctcgctt attttaatgg ctgttagtgt ggttgttttt
    21301 ttaacagcag atgtaacgat gaaactcctg attattacag caccgatatt tatcttttgt
    21361 ctcatgtttg gttttattcg gagcatgttt aataattggt tacaatcgtt attctccagt
    21421 attctgacag ttctatttgc cagtttagtg attcggatag caatggattt tcaagggatg
    21481 atcctttccc atgctattcg tgctgcacaa acagggaatg taaatcttgt atctacaggt
    21541 gctatgggct ttatggctgg gtttttgggg gctttattgg ttcttatagc taaaggtttt
    21601 gctgtccagt tagcaggtgc aggtgttgaa ggtgctgttc agggcgcagc catgatgggg
    21661 ctgggtgctg ctggtatggc tactggtaag tctctgatgc ttggcggtcg tgctggttta
    21721 ggttttggct tagggatggc cggaagaacg ggcatgaatt ctttatcagg gaaagcaggg
    21781 aatctgatgg ggcgtggtgc gcgaacagcc gctgaatggg cgggtgaaaa gggatatcag
    21841 gcgctggctc cgacaggtgc tttggggatg aaggcgagaa gacttgcatc cctggaaaaa
    21901 gcacgagcga ggaacgccgc atgaaaataa tgaaacgttt tttttatgtg atgtccctga
    21961 actggatact ggtgttcgtt gataaacata tcacctccgg atggagcagg aagagaaagc
    22021 taataaccca gcttgttatt ttaatcacgg gaattacctt atttaaggtc ttaggtggaa
    22081 tcagtcagag ctggttgtga catgcttcat tattttgtca tgatacttta ttttgtccta
    22141 atccttcttt taggagtgtt tacctgcttc gcaggaataa gacttggttt agggcttgcg
    22201 gggaaatgga gaaagggtat ggatcttcgc tttttgcttc ttattggtgg gattcttttt
    22261 cttcatcttg gcctttatct cttaaaggtg ttcgtgaacg cttaataagg tgaatttatg
    22321 cgatgtgtga ttcttttgtt tttcctgttt cagatttccg gatgtaccac aaggaataac
    22381 cttccttctg atgtttccgg cgaactggaa cccattaacc acagccaggt aatcagttat
    22441 gagtgaaaaa atcgaaaaga agattgagac agctaaatcc ttcgaaagac aactctatgc
    22501 agaaaatgaa cggtctaaga aaaatgcatg gcgtgttgca gtggcagcat cattactgtc
    22561 cttatgtctt ggggctgcaa tttgtgttct tgtgccatta aaagaaaaag agcttgctat
    22621 tgtggctgtt ggcgaaaaaa caggccgtac agaactgatt actcaggtta aacaggaaaa
    22681 gatcctgcaa tctgaagcac ttgggcggta ttttgtaaac acgtatatta ctttacgtga
    22741 aggttataat tatccgtctc ttcagtatga ttatgagact gtacagctat atagctcgaa
    22801 tacagtaaag gacgattatc ttcgtctgta taacagcgat atggctcctg ataaaattta
    22861 tcataataac ggttcttacg ttgcggttga ggtcatctcc aatattattt ctgacgcaac
    22921 cgcgccagat aaactggcct ctgtgcgctt taaaaaaacg acgagaaact tcacgaccgg
    22981 acaggtttca gtttcatact ggacggctcg tgtgacatat cgttttgaac cagaaaagtc
    23041 ggttaaaagt tccagtcgtg agcttaatcc tctcggtttt actgtaacca gttaccagac
    23101 agatcgggaa gtcagggggg aatgatgaag aaattattta ttgctacatg tttattgctt
    23161 cctgggcttg tgtattcagc agcgactccg ttaccttctg gatttgatgc aaggatgcag
    23221 actgtgagtt ataacggtgc gaatacgacc gttatccggt caaagacagg atttctcaca
    23281 tcagttgttt ttgatgaagg tgaggctgtt atcagtgcaa aggctggttt tcctgccgga
    23341 tgggaaatca caaccgatga taatgttgtt tacatcaatc cgcgtcccgt tgttcaggaa
    23401 caggaaggcg atgaaggcga aaaactgaaa aaggttttcc agccaacaga aaaggaatgg
    23461 gatacgaatc tttttgtcag aacaacaaag cggatttaca gtctggatct aatccttctg
    23521 tcagaagaaa aacaagcgca acctgcttat gttgtacaat tccgctatcc atcagaaata
    23581 gcgaaaaaga atgcagaaga agtaaggctg gcaaaagaaa aacaggaaaa actcagacag
    23641 aagaaactga tttctgaaag ttttgaaaaa gccgatgcgc ctaaaaactg gaattatttc
    23701 atgcgagtca atgaaaaata tgacagtcgt cgtatcgccc ctgattttgc ttatgataat
    23761 ggcatattta cctttttagg ttttaactca gggaaagtat tcccagctcc atttgctgta
    23821 agagatggtc aggaacagac acttgctttt aatgtggaaa caaaaggcaa gtataaaatt
    23881 atggttatcc acaacgttaa tgacaaattt gttttacgct atggaaacag cgttgttggt
    23941 gttgtgaata aatctttcgg aaaagtatta accgaccaga ggaatacatc ttctcctgcg
    24001 gtagaacgtg ttgaggtgaa taatgactga tatgaaaaaa acagatgatt tagttgatga
    24061 tgcgaatcag tcaaagctgg aaagtaaacc cgttcatgat atcggtgata tcaggaacaa
    24121 aaataatcgt atgcgttctg gctttctgtt tggtctgatg gctgtattag ttattggtat
    24181 ttttgcgctg aaaacatata aaaattattt tgcagatgac cagcagaaag catcagaatc
    24241 aacaggcgat accagcattt ctcaggtaag taaaatccgt actggtcttg gtcagaattt
    24301 tgaccctgtt gagaacaaag caattatcaa tccaggcgct actggttctg gtaatactgt
    24361 ttctgacggt cataaagaag tgccacagga ggggttcagg aaatatttgt caatacctgt
    24421 tgctggtcag ggagggagtc agactggcgc gagtggcagc agcaggacat cgcagacatc
    24481 agagccacag gaagaaagga aagaatcaaa agaaaatgtg cctggtaaat caggaatgaa
    24541 agtaactgcg attaatctcg atcctgatct ctatattgag gaaaacaggc ttataccgtg
    24601 tgctttaacc acacgctttg tttctgatgt tgcaggtcgt atcagttgtg tttttacaga
    24661 agatgtatgg agtgctaatc accacacaaa attactggaa cagggaacta aggcttttgg
    24721 tcgttaccag accggaacgc tgaatcatgg acagggcaga atgttcgtta tgtgggagca
    24781 gttgcggacg cctgataata aacgaatcga tatggtaaat acagcagcag ctggtcccct
    24841 gggagaagcg ggcattgatg gatggatcga ttctcacttc tgggagcgtt ttggtggttc
    24901 tcttatgctc agtatggttc aggatgtagc ggcggcggcg gcggataatg cacctggtaa
    24961 agatcgtaat gtggattata ccgaaaactc acgacaggca atggctgaaa tggctaaagt
    25021 cgcacttgag aattcgataa atattcctcc aacaatgtat aaaaaccagg gtgacataat
    25081 cactattatg gtcggggaag acattgattt ttctgatatc tatgagttaa aggtaaaata
    25141 gtatgagtgg cgttattcct gtcagcatta gcaataaatc gctcgatttt tataaaaatc
    25201 aagtattcca gtcttttctt gatattgaag ggctaacaga aattgctgtc aaccgtcctg
    25261 gtgaaatctg gacggaaatt aatggtgact ggacattcca taataatgac agtgtgacct
    25321 ttgatttttg taaacgtttt tctcatacgc tggcatcatt tcggggagat gaaatcggcg
    25381 agaccaagcc attactttca gcaacactgg aatcaggaga acgtgtacag gtcgtttttc
    25441 ctcctgcatg tgagagaaat actatttcaa tcactatacg taaaccatcg acacggcaga
    25501 taacacataa agaatatgtg gcgaacggtt tttatgatta tgtccagtca ggaaaaaaac
    25561 acaggacata cgatgatgag cttcttgatt tattcagagc gagaaatatc gctgctttta
    25621 tggagcttgc cgtagcagca gggaaaacca ttgtatttgc cggagcaacg ggttccggta
    25681 aaacaactta tatgaagagt cttatcgatt tcataccatt gaatacacgg ctgattacga
    25741 ttgaagatgc agaggaaatc aaatttttta tccataaaaa ttatgtgcat cttttttacc
    25801 cttcggaatc cggcagtgat tccggctcta tcataacagc agcgaaatta atcaaatcct
    25861 gtttgaggat gaaacctgac agaattttac tggcggaaat taaaggtggc gatgcctggg
    25921 attttgtgaa agttgctggt tctggtcaca gtggaagcat gacgtccatt cacgcggcct
    25981 cggcaaaaga tgccatcatt cagatggtga caaaatgtta tcagaacaga gaatgtcaga
    26041 atcttccttt tgatgttctt cgtaaaatca ttatggacag tattgatatt gttgttcatg
    26101 taggcaggga tggtgttgtc aggcatatga gcgacatcta ttacaaaggg gctgaatgtg
    26161 aaaactttta ataagtcgca atctgttttt atattcgctg ttatgcttgt tgcggcatgg
    26221 tttgcaggtt catttatttt ctttgtgctt tacttcagcc agataaagaa tctgaaacca
    26281 ttgaaggctg tgttcagatc cattgatgcg tacaggatgg atatttttac agacagcctt
    26341 actttatctg ttgtgactac ggatatcaga gttttagcct ttgttgcact tggagcagga
    26401 tttgtcgtgt ctcttatcat ccctgttgcg gcgttgataa aactgaacga gaagaaagaa
    26461 aatattttcg gtgatgcgcg atttgcaaca attaatgata ttcgtgagtc taacagtttc
    26521 acgcttgacg gtgacgaaaa agacgggatt atcgttggta taaaagacaa gaaaatcata
    26581 cgctatgtgg gagcagcatt cagtgctatg ggagccggaa caagggctgg taaaggtgct
    26641 ggtatagtca tcactaatct tatgaagtac tggtggagtg ttattattct cgatccaaaa
    26701 agggagtgtt ttaatatcac cagtcttatc aggaaggtga ttttaggtca tgaggtttat
    26761 aaatttgatc cctttagttc ggtaacgcac cgctttaacc ctttgtatta tgtcagcatg
    26821 ggaacgagtg aaggttttaa tcagcttgaa aatctttcgt tgattattta cccctataag
    26881 acagatggtg cggatgctgg tagctatctt aataagacgg cgggtggtgt ttttaaatct
    26941 tatgctgtcg ctttatggtt tatgattaag aatgataagg ctggactaaa aacgcttgat
    27001 attgagcctg tcttctcaat gtctaaaatt aatcagcttt ttgagcgtgc agaacccgaa
    27061 catcttcttt cattcatgaa tgatattcgt tcagaattaa gggggaaaga caaaacgctt
    27121 gctgatattg gtgtcgctgg ccttaaggca ttcattgaga tggaggataa aacaaaagca
    27181 caactcaaaa gcaactttct taatggatta tcaccgtttg ctaaccccaa tgtggcaaat
    27241 gctactgatg gaaatgattt tgatttgcgg caggtgcgga aaaagcggat gacaatttat
    27301 ttctgtattt ctggtgataa tgccaggttg gctgaaaaaa taacaaatat tttctttcag
    27361 cttgctatac aggtgaatct tgaaaagatg ccaacggatg atccggaaat aaagcatgac
    27421 tgcctttttc tactggatga attcccgtca attggtgctg tagattacat caagagtaaa
    27481 tctggtctca ttgctggtta caagctgaaa ttgttgattg tttaccaggt cggctctcag
    27541 cttgaggaaa tctacagtta tgcaggcagt aaaacattac tggcaagtgc accctgcaaa
    27601 attgtttatt ccgcttctga tgttaaagat gcgcgggagt tatcagaagc gatggggaca
    27661 cgaacagtaa ctatcggttc gaaaagcaaa agtcgtagtc gtggaggaac atcgagatct
    27721 gaatcagaaa gccttattga gcgtccactg gtaacaacaa atgaactact tacactgaaa
    27781 tttagtgaag aaatcctggc aatgaaggga gaaaacccga ttcgttgtga aaaggcattc
    27841 tactatctca atgagtattt ctttggtgac tttgtgaagg ttgctccgga acttgcaaac
    27901 attttcccca ggaagaaagg aaagctggtt atgcctccac agaaagtatt tgaaaatgaa
    27961 atcgtcgcaa aagggtatct ggtagtaaaa gatgttcctg atctcgataa agcggcataa
    28021 aggagaacat gatgaagaag ttagtgtttt tattgatttc cattcttgct ggttgttcat
    28081 caccgccaga gccaacaccc gtgcagtttg aaaaggcaaa tgaagtgatc aatccctcat
    28141 tgccatatgt ccccgatttt cacggtgtca taaagtcaga cgttagcggt aaaggatggg
    28201 tttatgagat cacttcctta tccggagtac aggccagaac gccgacgttt tattacgctc
    28261 tggctcatgc ggacaggatt gtagtgacaa cacatgatgc tgggctgtgg tttcgaatca
    28321 gagatctgtt gaagatggaa ggggcaacag cagtcgtgga atggcgaaac gagaaaagtt
    28381 ttttacctga acaggtaaaa atagttttca ttaaagcaca aaatgaggtg aaaaatgact
    28441 ggaaaaaata gagtggtttt accagccttg ctggcgttag gattaatatc aacaaatgct
    28501 aatgcatctg acccttgcgc ttcggtactt tgcttatatg gaaaagcagt cgggcaaggt
    28561 ggagggagtg aatgcagaag tgctgaaaag gattttttta atattctgaa aaagaaaaaa
    28621 ggaagcattc gctggagtaa aacttttgat gcccgtaaag catttctgaa tcagtgttca
    28681 acggctgatc ctgctgctat atcaaaaatc atgagtaagt ttggtcgtgt cagaggttaa
    28741 ttaaaacctg ataaccgcgc caacgggtat cagggtactg caagtatcac atttcgcgcc
    28801 aacgaaatgt gatgattagt tacaatcact gaggttagaa atgaaagtaa ccaaatctat
    28861 tatagctatt ttattgtgct tatctgctgg tagtgcataa gtggatgtaa taaaaaatgc
    28921 tcgtgccttt ttgaaaggaa ccctcgatct gagcgttcag gaagcagatg aagatataga
    28981 ggaaagggaa gaactttata aaaagaacgg ggcacaacct gactatttaa gttacctgga
    29041 ggattaacac atgcaatatg cattgtttga tggatttgaa cgaaagtttt tgctggatgc
    29101 tcttgaattc ggtgttctga aggactggaa agaaaatccg gtaaaagaac ttcctgatat
    29161 tgatgaatct gctcacccct tccatatctg ttatggtgga tatttattaa accctggtgt
    29221 ttcagattca gatattagca gaaaaataaa agaccaggca ggattctggc tggcagctat
    29281 tgatgatacc cgcatggatt gtcattcaat agcttattat gatattcaca cactccctct
    29341 gatttcgtgt ggtcatcaaa agatagttcc ttttgccgcg ttaataaaag ctgatgaatg
    29401 catcatttca aaaattgctt cgtattctgg ttttgctgta acagcctttt tgagaattaa
    29461 agagtgggat attgcaacta atatacttaa ccgtgaggga atttttgcct ttaatggctg
    29521 tgagcgcaga ttcagagtag taagtaaaga taactggcaa catacagtat cggaagaacg
    29581 cgctatccgt tgtgccaaaa gattaattca atgtaaagga taacaaaatg agacttttta
    29641 tcgcagaaaa acccgcagta gcaaatgata ttgttaaggc acttggtggc aattttaccc
    29701 gccatgatgg ctggtttgaa agtgataacg ccattgtgac taactgtttt ggtcatatta
    29761 tcgaatcaca accgccggaa aactataatc ctgaatacaa agcctggaag gttgaaacgc
    29821 ttcctttacg tctttatccc gtgaagtatc agcctgttga aagtgcagca aaacaggtta
    29881 aaacgattct cgaacttatc agacgtggag acgtgactga aattgttcac gctggcgatc
    29941 ctgatgatga gggacagcta ctggttgatg aagtcctgga atatgcaggc aacacaaaac
    30001 ccgtaaagcg cgttctgatt aacgacaaca cgcttccggc agtgaaaaag gcactggcaa
    30061 atcttaaaga caatcgtgat ttcaaaggac tttaccttaa ggcgttggcg cggtcagttg
    30121 ccgatgccgt ctatggcttc tctatgacac gtgcgtacac cattccggca aaagccagag
    30181 gatatcaggg cgttctgtct gtcgggcgcg tccagactcc cgttcttggc ctgattgtga
    30241 atcgtacccg tgctaaccag aaccataaat ccagttttta ctacaccatg accggagtct
    30301 ttcagcgtgg tgctgatgtt atcagggcga actggaagcc aggggaattt gctccgctga
    30361 cagaccgtaa attacttgat aaagcgtggg cagacggaac ggctgcatcc cttgcaggaa
    30421 aaccggctac agttgaagca gcagcaactg atgataaaaa aacggctgcg ccgttgccgt
    30481 ttaatctggt cagactccag caatacatga acaagaagtt taaaatgacg gcacaaaaaa
    30541 cgctggatat tacgcaacaa ctacgcgaaa aatataaagc aattacgtat aaccgctctg
    30601 actgctcata tctttcagat gaacaattca gcgaagcgcc gcaggttatc gatgccctga
    30661 aatcagtctt tcctcagtcg ctggatattg attctgcacg taaaagcaaa gcgtttaaca
    30721 gtgcaaaggt gactgcgcat actgcgataa tcccgaccgc cagtgtgcct gatgttaacg
    30781 cactcagcac cgacgagcgc aatgtttacc tggcgatcgc acaacactat cttgttcagt
    30841 tcatgcctga aaaagcatac caggaagtat cggttgccat tcagtgtggt gatgagtcgt
    30901 tctatgcccg tgccagaaaa acaactgaca gcggatttga ggcgtttctt ggcgcggaaa
    30961 ccacagacga aggtgaatca gaagataatg atgattccgc ttttgaactg ctctgtaaaa
    31021 ttcgcacagg agaaacactg acgacaaaag aagttattgt taatgagaag aaaacaacac
    31081 cgccgccgtt attcaccgaa gcctccttgc ttgctgcgct tgttcgtgtc gcggattttg
    31141 tcactgatcc aacaattaaa aaattgttga aggataagga taaagacaaa aaagatgaac
    31201 atggtggcat tggtacgcca gctacccgcg cagccattct ggaaacgctg aagaaaagaa
    31261 actatatcac gctggaaaaa gggaaactta ttcctactga taccggatat gcgcttattg
    31321 atgccctgcc tggtatagcg gttaatcctg atatgacagc attatggtct gaaaagcagg
    31381 ctgccataga aaatggcgac ctgacggttg aacagtttat taatgatctg tacggtgaac
    31441 tgacaggcat gatttctgat gttgacctgg gcgagatgaa gattgaaccc gctgcgccag
    31501 cagggcagtt tcaacgcctg gactctccct gcccttcctg tggtaaacat attgttatca
    31561 ggccgaaagg ttatttctgt accggatgtg aatttaaaat ctggagtgag ttttctggta
    31621 agaaaatcac ccaggcacag gccgaaaaac tggttaaatc agggaaaacc gatttgatta
    31681 agggatttaa aaagaaaagt ggtggaacgt atgatacagt tcttgtcctt gaggataaga
    31741 aaacagggaa gctgggtttt ccggcaaggg ctaagaagtg aaaacaaagc aggaatggct
    31801 ttttcagtta agaaaatgta catcaagaga tactcttgaa aaagttattg agattaaccg
    31861 ttacaaactg cctttatcag aatcagaggc attttattct gccgcagatc atcgccgagc
    31921 agaactggtg atgaataaac tttatgataa ggtgccttcc ggcgtctgga agtacgtaca
    31981 ctaaataaga ggattaatta tatgagcgaa ctgactaaag aagatgaata cggcattatc
    32041 agccgtacta tgatgaatat tcgttcattg cgtgtttttg cccgtgagat tgattttgag
    32101 cagttgctcg aaatgcagga aaagctcaac gttgttattg aagaacgtcg tgaagatgct
    32161 gaacgtgaag cggctgaacg ccaggaactt gaagccaaac gtcagcaggc cattgaatac
    32221 atcatatctt taggtcttga tcccgaatca ctccttgctc ctgtaacagc agatacagta
    32281 aaaaccaggc ggaaagccaa aggcggagta agaaaggcta aataccgctt taaggatgaa
    32341 aatggcgaga tcagagaatg gtctggtaat ggcaagcgtc cgcttgcttt acagaagctg
    32401 cttgatgagg ggcatttcat ggaagatttc ctcattgaga aaactaagcc ggaacaggca
    32461 gaataacccg ccaagtatac atcacacagg gcactgttgc aaatagtcgg tggtgataaa
    32521 cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc
    32581 agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg
    32641 agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg atttaccgct
    32701 gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg cgtaaccctt
    32761 ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc cgctgggcgt
    32821 atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc tcctcccgtc
    32881 gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg aagaagtggc
    32941 agatcccgcg attcatcaac acggataaag cgcccgccta tggtcgcgcg cttgctctgc
    33001 tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag taccggaaca
    33061 acgtgattga atgcgatcat ggcaaactga aacggataat cggcgccacg ctgggattta
    33121 aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt gcactacgca
    33181 aaggccaggc ctcagcattt tattatggtg atcccctggg cgaaatgcgc ctggtaagca
    33241 gagtttttga aatgtaaggc ctttgaataa gacaaaaggc tgcctcatcg ctaactttgc
    33301 aacagtgcc
//
