LOCUS       NZ_KY454616             6141 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain kNICU5 plasmid pkNICU5, complete
            sequence.
ACCESSION   NZ_KY454616
VERSION     NZ_KY454616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227542
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Yin,D.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2017) Department of Nosocomial Infection Control,
            The Clinical Microbiology Laboratory, Wanyuan Road 399, Shanghai
            200000, China
COMMENT     ##Assembly-Data-START##
            Sequencing Technology             :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 01:46:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KY454616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="kNICU5"
                     /isolation_source="sputum"
                     /db_xref="taxon:573"
                     /plasmid="pkNICU5"
                     /geo_loc_name="China"
                     /collection_date="2016-04-13"
     gene            join(6000..6141,1..197)
                     /locus_tag="HTK86_RS00005"
     CDS             join(6000..6141,1..197)
                     /locus_tag="HTK86_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            292..1632
                     /locus_tag="HTK86_RS00050"
     CDS             292..1632
                     /locus_tag="HTK86_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_228283704.1"
                     /translation="MIEALIDSSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLP
                     GLEGNQYAVLWVEHRDKGRLELNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGE
                     LKLHDPDDPLNRQAVITPKDLPRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAG
                     FSVVRETKNSLSIADPDGGRNIRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIH
                     EARESYLRGVEIKRGENERRYCRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGG
                     IDSGAASHSPSLGREGRQAKADPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDG
                     IGAGIALRVRGFAERIRAAAQGMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLN
                     AASGRLEQAAGGLDRASQQVAKEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1914..2195)
                     /locus_tag="HTK86_RS00025"
     CDS             complement(1914..2195)
                     /locus_tag="HTK86_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            2539..3336
                     /locus_tag="HTK86_RS00030"
     CDS             2539..3336
                     /locus_tag="HTK86_RS00030"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            complement(3743..>3895)
                     /locus_tag="HTK86_RS00035"
                     /pseudo=""
     CDS             complement(3743..>3895)
                     /locus_tag="HTK86_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            4271..5218
                     /locus_tag="HTK86_RS00040"
     CDS             4271..5218
                     /locus_tag="HTK86_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            5626..6018
                     /locus_tag="HTK86_RS00045"
     CDS             5626..6018
                     /locus_tag="HTK86_RS00045"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     ARG             2540..3333
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4284..5205
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          179..1629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agcgccgaac gccggcgcct accgtagatc cggcgctctt gcggcagttg gccggaatgg
       61 gcaacaacct gaaccagatc gcccggcgcg ttaattccgg ggagtggggg ccgttggatc
      121 ggctgcgcat catagcggag ctgtccgcta taggccgcga gctggaggaa ctgcaccatg
      181 atcatcaaat tccataaccg tggggtaggc cggggctccg ggcctgttgg ctacttgttg
      241 ggccgtgacg ggaaccggga aggcgcaacg ctcgaccggg gcaaccctgc ggtgattgaa
      301 gccctgattg attccagtcc ctacgccaaa aaatacacct ctggcgttct gtcgtttgcc
      361 gaggctgatt tacccagggc gaccaaggac aagctgatga gcgactttga aaaggcgctc
      421 ctgcccggac tggagggcaa ccagtacgct gttttgtggg tagagcaccg cgacaagggg
      481 cggctggagc tgaattttgt agtcccgaac atcgaattgc agagcgggaa gcggctgcaa
      541 ccctacttcc acaaggccga caatccgcgc ctcgatgcgt ggcgaaccgt ggtaaacggg
      601 gagctgaaat tgcacgaccc ggacgatccg ttaaaccgtc aggccgttat cacgccaaag
      661 gatttgccga gggggcacaa ggaggccgct caggccatta cagacgggct tttacgcatg
      721 gccggagcag gggagatcca gagccgcgat gatgtgcgca aagccttgca gggtgcgggt
      781 ttcagcgtgg tacgcgaaac caaaaacagc ctgagcattg ccgacccgga cggggggcgg
      841 aacatccgat taaagggggt gctgtatgag caagatttta gacatggcga aggacttcga
      901 gcagagctcg aagcagcagg ccaacgctat cgagaagcgt ctgaccgaag aattcacgag
      961 gcacgagaaa gctatctccg gggcgttgaa atcaagcgag gagaaaatga gcgccgctat
     1021 tgccgagcac agccagaaaa tgggctggct gttgctcaaa acgtggatct ggatgctggt
     1081 cagcatcgtc ctgttcttga gcgcaacgag cgggatcctc tggtatcagg gggaattgat
     1141 agcggagcgg caagccactc tccaagcctt ggcagagaag ggcggcaagc taaagctgac
     1201 ccagtgcggg aagaaattgg agcacctttg cgtgcagatc tccgacccca atccaaccgt
     1261 ttgggggaag gatacctatt ggatcgtgcc gaaggggtac taaatgacgg gattggagcg
     1321 ggaattgctc tgcgcgttcg aggctttgca gagcgcatac gagcagcagc acaaggcatg
     1381 gcaggaaagc tacgcgaact tggcgagcat gtacgagagc accaaacaag aggccgagat
     1441 cagcagggca gcagcagagc gattgacgag gcaggtgaac ggcttaacgc agcaagtgga
     1501 cggcttgagc aagcagctgg agggcttgac cgggctagtc agcaagttgc aaaagaaagg
     1561 gaacacaggc tcctgaaggc actggatcga ggctgggagc gtggagacag atcgcgaggc
     1621 tttgagcgtt gacggaacaa aacggccgtc agttataaaa aaaggaaagt gctatcactt
     1681 tccttttatc ggcgggaatt atcgttgagt tcttctatgc catttttcat agtagtaact
     1741 gtaatcttca tctctaccac cagattcaat ccagtcatca tactcagggc tatttttgaa
     1801 atctgaatca gacatgctaa ctccttgtta ttagttggct tctatggaag ctagcgcacc
     1861 gtgaagttat gatcaacgat gtagtccaca tcattgtcga aatgtgcgtt cagctagtct
     1921 tcggtgtttt ctgctttatt tctagcggcg attctcgcgt ctgcccatgc ggcagacttt
     1981 ttagcctgtt ctcgttgttc ttcggttgaa gactcgctgt tgagaacttt gttgctcgtt
     2041 tgtttgacca ccgaatccaa acctgttgtt gctgcaaaat gagctaggtt aacaactccc
     2101 ttacctaaca acttggcact agcgattgcg gtttctttag ccccaaaagc atcagacacc
     2161 tcctcaagcg ctttagatag gttaaaaaat cccatactcc ccctaagaat atcatcaata
     2221 aaattgagtg ttgctctgtg gataacttgc agagtttatt aagtatcatt gcagcaaaga
     2281 tgaaatcaat gatttatcaa aaatgattga aaggtggttg taaataatgt tacaatgtgt
     2341 gagaagcagt ctaaattctt cgtgaaatag tgatttttga agctaataaa aaacacacgt
     2401 ggaatttagg taatagttaa agcttgtgcc tgattgttgc cacaattggt cagcagtttg
     2461 ctagggaatg agagggcaga gaatgataag cttagcactt aatttgtatg gatatattgc
     2521 attaagcaag gggacgttat gcgtgtatta gccttatcgg ctgtgttttt ggtggcatcg
     2581 attatcggaa tgccagcggt agcaaaggaa tggcaagaaa acaaaagttg gaatgctcac
     2641 tttactgaac ataaatcaca gggcgtagtt gtgctctgga atgagaataa gcagcaagga
     2701 tttaccaata atcttaaacg ggcgaaccaa gcatttttac ccgcatctac ctttaaaatt
     2761 cccaatagct tgatcgccct cgatttgggc gtggttaagg atgaacacca agtctttaag
     2821 tgggatggac agacgcgtga tatcgccgct tggaatcgtg accatgactt aattaccgcg
     2881 atgaagtact cagttgtgcc tgtttatcaa gaatttgccc gccaaattgg tgaggcacgt
     2941 atgagtaaaa tgctgcacgc cttcgattat ggcaatgagg atatctcggg caatgtagac
     3001 agtttttggc tcgatggtgg tattcgcatt tcggctaccc agcaaatcgc ttttttacgc
     3061 aagctgtatc acaacaagct gcacgtttct gagcgtagtc agcgcatcgt gaaacaagcc
     3121 atgctgaccg aagccaatgg cgactatatt attcgggcta aaacgggata ctcgactagt
     3181 atcgaaccta agattggctg gtgggttggt tgggttgaac ttgatgataa tgtgtggttt
     3241 tttgcgatga atatggatat gcccacatcg gatggtttag ggctgcgcca agccatcaca
     3301 aaagaagtgc tcaaacagga gaaaattatt ccctagaagt ggttagcgcg tatttgtgtg
     3361 aaatagccgt catataagct gtaaagatat atggacaaaa tgctgatgct cgatgcgctt
     3421 gtagttaatg gctcatactt aatcggtcag gcctaatcga cttaagctcc gcgcgttaac
     3481 tttcagcctg agcattagca tcacattcct aaacgtaaaa agcctagcca atttgctagg
     3541 cttttttgtt atctcaatgg ccatggtcac tcaacatggg ggtgtgaata aaggagtggc
     3601 gatgtcagta aaggtgaatg ccgctgcgat aaagtgccta gcccatcaag gattgtaggc
     3661 tttcttggct gctaaagcgt ttgacgaaaa agtccaagaa ggcgctgata ttggttctcc
     3721 atcattttca tctaccggtt tactataggt caaccaggcc gtccttgccg gcgcttggga
     3781 cgcagacgat ggcatcaaat gcctcgctca aattcgtttc tacagaggcg ctttgggacc
     3841 gcattcgctt tgctttcatg ggggcatctg tcaatgtcag acatgaatcc tccgatatag
     3901 gttgattttt gatctcttca acacttggtg ttgtccgact agagagactt atatggatca
     3961 gtattcttgc actgacaatg gtgccactca atcccaagga tcgacctagt tagaggcgta
     4021 gatcaagcaa ttcaaaacag cgcagaagca gaaggaggtg gaaatatgtt ccagggtgaa
     4081 aggttacatg caactaacgt tgctctctag gatgtgcctg ctgtaacgta ttgtccagaa
     4141 aagacaaacc cccgttatcg cttgcatctt ggcggagctg cgaaaaacgg gggccgtgac
     4201 gaatcacaag agaagtctat cacatggatc atgccaaaca accccacgcc gccaataagc
     4261 gcggtggtgc ttgagaaagt gcgcacagct tgagctgttt cgggagcgcc tgccccggaa
     4321 accctactat tccgacgagc ttacaacggg cctacggatt gcggatgttg cccgcgccct
     4381 tggtgcgcgt tacatccagc cgaacgggcc aacgcaccgc cactggatcg tttttgacgt
     4441 agaccatgca gccgctacgt tatcttggga tgacgtaggc gctccagccc ctaacattac
     4501 cgtcacgaac aaggccaacg gccacgcgca tctgatctac ggactggata cgccgatccg
     4561 cactgccccg gatggaaatg cagcccctct gcggtatgcc gccgccatcg aggccgcgct
     4621 ccgggagaaa ttaggggcgg atatgggcta ctccgggctg atctgcaaga accccctgca
     4681 tgagcactgg ctggtgcagg tatgggaacc ccggctctac gatttagcct ggctatccga
     4741 ttacctcgat ctgagctcct acaatggccg caaatcgctt cctgagtacg ggcttgggcg
     4801 aaactgtacc ctgttcgaga aaacgcgcct gtgggcctat aaagcgatcc ggcagggctg
     4861 gccggattac cccgaatggt tggccgcttg tgttgaccgg gcatcaggat ataacgccca
     4921 gttcgagcag ccgctccccg caaacgaagt ccggcacacg gccaagtcca ttgccaaatg
     4981 gacgcaccag cacctaagcc ccgcagggtt cagagaggag caggcccgga gaggtgcgaa
     5041 gggcggcaag gtatccaagg gagggggaag gccgagcaat agcggtaagg acaagtccga
     5101 tctattgccg gaggttctgc ggcttaaggc tcaggggtac accaaccgcg atatagcgga
     5161 cgatcttcaa atctccccca gtacggtgag cgtttaccta aagcgtgatc gtccgtgatc
     5221 gaaacaagcc ttatcagata atagccccct ttaggggggt tcctgcgctc ttgtctttta
     5281 gccttaaaaa atccttttag cgcggatctt tggcgaggtg gacaactgcg cctgtccgtc
     5341 catcgccccc ctgttttttc ggggggtgtg ggcggcagca gttatccacg gagccgctct
     5401 ttcccttgat cttgctctag ggcttgcagt atcccgccca tggttgaata atgtactaag
     5461 caaggctaca cagaggcccc tctacctgct tcgcggtctc gggcctctgt ggccttgttc
     5521 aggggtgcta cgcccccccg aaaccccctg tggcccccgc tcgatccgcc ccttcgggac
     5581 ggtctgcgcg gtggcttctt ggctctggag aatggacgag gccctatgtc aacaacacag
     5641 atagagaaag attcgagaag gaaggcagtc aatggcgtta tttctagttt ccaactagag
     5701 ggggaggcca taaccgatga attcaaaatt ctacttgaag attatatcaa tggaaaaatt
     5761 tccaccgatg aggtgttagc tatggtaagt gacgaaccca caacaacgga atacgactac
     5821 gaaatcaccc cccgcactct cggtcttggc gggggctgga atctgcgttt gctggagaac
     5881 ggcgaagagg tcggcggggg tgttttcccc ttgccggagc actgcgattt tcgggatgaa
     5941 aaggcgctcc agacgctact cgatagcctc tatgaggacg ccttagcgga ggcctctgca
     6001 tggctggcca gccgttaaag cgcctccgct ctatcaagat ccgggtctca gatgcggagc
     6061 ttgagcgcct gcgggagctc tgccccaagg cccaactggc ggagtggatg cgggagcaat
     6121 gtctcggtgt tgcgcaaccc c
//
