LOCUS       NZ_KX555452            34149 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Escherichia coli plasmid pMCR-13EC-C962A, complete sequence.
ACCESSION   NZ_KX555452
VERSION     NZ_KX555452.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227062
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34149)
  AUTHORS   Ho,P.L., Lo,W.U., Wang,Y. and Chow,K.H.
  TITLE     Complete sequencing of a IncX4 plasmid, pMCR-13EC-C962A encoding
            MCR-1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34149)
  AUTHORS   Ho,P.L., Lo,W.U., Wang,Y. and Chow,K.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2016) Microbiology, The University of Hong Kong,
            University Pathology Building, 102 Pokfulam Road, Hong Kong, Nil
            Nil, Hong Kong
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: CLC genomic workbench v. 8.5.1
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:40:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 41
            CDSs (total)                      :: 41
            Genes (coding)                    :: 39
            CDSs (with protein)               :: 39
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 1 of 2
            Pseudo Genes (multiple problems)  :: 1 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX555452.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolation_source="chicken feces"
                     /db_xref="taxon:562"
                     /plasmid="pMCR-13EC-C962A"
                     /collection_date="2013"
     gene            863..1729
                     /locus_tag="HTJ20_RS00005"
                     /old_locus_tag="pMCR-13EC-C962A_01"
     CDS             863..1729
                     /locus_tag="HTJ20_RS00005"
                     /old_locus_tag="pMCR-13EC-C962A_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            1742..2146
                     /locus_tag="HTJ20_RS00010"
                     /old_locus_tag="pMCR-13EC-C962A_02"
     CDS             1742..2146
                     /locus_tag="HTJ20_RS00010"
                     /old_locus_tag="pMCR-13EC-C962A_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            2281..2808
                     /locus_tag="HTJ20_RS00015"
                     /old_locus_tag="pMCR-13EC-C962A_03"
     CDS             2281..2808
                     /locus_tag="HTJ20_RS00015"
                     /old_locus_tag="pMCR-13EC-C962A_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(3199..3945)
                     /locus_tag="HTJ20_RS00020"
                     /old_locus_tag="pMCR-13EC-C962A_04"
     CDS             complement(3199..3945)
                     /locus_tag="HTJ20_RS00020"
                     /old_locus_tag="pMCR-13EC-C962A_04"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_104772353.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQINLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(3993..5618)
                     /locus_tag="HTJ20_RS00025"
                     /old_locus_tag="pMCR-13EC-C962A_05"
     CDS             complement(3993..5618)
                     /locus_tag="HTJ20_RS00025"
                     /old_locus_tag="pMCR-13EC-C962A_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            complement(5792..6421)
                     /locus_tag="HTJ20_RS00030"
                     /old_locus_tag="pMCR-13EC-C962A_06"
     CDS             complement(5792..6421)
                     /locus_tag="HTJ20_RS00030"
                     /old_locus_tag="pMCR-13EC-C962A_06"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            complement(6582..6851)
                     /locus_tag="HTJ20_RS00035"
                     /old_locus_tag="pMCR-13EC-C962A_08"
     CDS             complement(6582..6851)
                     /locus_tag="HTJ20_RS00035"
                     /old_locus_tag="pMCR-13EC-C962A_08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(6926..7573)
                     /locus_tag="HTJ20_RS00040"
                     /old_locus_tag="pMCR-13EC-C962A_09"
     CDS             complement(6926..7573)
                     /locus_tag="HTJ20_RS00040"
                     /old_locus_tag="pMCR-13EC-C962A_09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(8236..8331)
                     /gene="dqlB"
                     /locus_tag="HTJ20_RS00210"
     CDS             complement(8236..8331)
                     /gene="dqlB"
                     /locus_tag="HTJ20_RS00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(9164..9628)
                     /locus_tag="HTJ20_RS00045"
                     /old_locus_tag="pMCR-13EC-C962A_12"
     CDS             complement(9164..9628)
                     /locus_tag="HTJ20_RS00045"
                     /old_locus_tag="pMCR-13EC-C962A_12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(9646..9852)
                     /gene="hha"
                     /locus_tag="HTJ20_RS00050"
                     /old_locus_tag="pMCR-13EC-C962A_13"
     CDS             complement(9646..9852)
                     /gene="hha"
                     /locus_tag="HTJ20_RS00050"
                     /old_locus_tag="pMCR-13EC-C962A_13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            complement(9849..12002)
                     /locus_tag="HTJ20_RS00055"
                     /old_locus_tag="pMCR-13EC-C962A_14"
     CDS             complement(9849..12002)
                     /locus_tag="HTJ20_RS00055"
                     /old_locus_tag="pMCR-13EC-C962A_14"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            complement(12007..12579)
                     /locus_tag="HTJ20_RS00060"
                     /old_locus_tag="pMCR-13EC-C962A_15"
     CDS             complement(12007..12579)
                     /locus_tag="HTJ20_RS00060"
                     /old_locus_tag="pMCR-13EC-C962A_15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(12583..>12777)
                     /locus_tag="HTJ20_RS00065"
                     /pseudo=""
     CDS             complement(12583..>12777)
                     /locus_tag="HTJ20_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            complement(12890..13195)
                     /locus_tag="HTJ20_RS00070"
                     /old_locus_tag="pMCR-13EC-C962A_16"
     CDS             complement(12890..13195)
                     /locus_tag="HTJ20_RS00070"
                     /old_locus_tag="pMCR-13EC-C962A_16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(13179..13601)
                     /locus_tag="HTJ20_RS00075"
     CDS             complement(13179..13601)
                     /locus_tag="HTJ20_RS00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(13610..15472)
                     /gene="taxB"
                     /locus_tag="HTJ20_RS00080"
                     /old_locus_tag="pMCR-13EC-C962A_17"
     CDS             complement(13610..15472)
                     /gene="taxB"
                     /locus_tag="HTJ20_RS00080"
                     /old_locus_tag="pMCR-13EC-C962A_17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            complement(15459..16487)
                     /gene="virB11"
                     /locus_tag="HTJ20_RS00085"
                     /old_locus_tag="pMCR-13EC-C962A_18"
     CDS             complement(15459..16487)
                     /gene="virB11"
                     /locus_tag="HTJ20_RS00085"
                     /old_locus_tag="pMCR-13EC-C962A_18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(16489..17607)
                     /gene="virB10"
                     /locus_tag="HTJ20_RS00090"
                     /old_locus_tag="pMCR-13EC-C962A_19"
     CDS             complement(16489..17607)
                     /gene="virB10"
                     /locus_tag="HTJ20_RS00090"
                     /old_locus_tag="pMCR-13EC-C962A_19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            complement(17600..18508)
                     /gene="virB9"
                     /locus_tag="HTJ20_RS00095"
                     /old_locus_tag="pMCR-13EC-C962A_20"
     CDS             complement(17600..18508)
                     /gene="virB9"
                     /locus_tag="HTJ20_RS00095"
                     /old_locus_tag="pMCR-13EC-C962A_20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(18505..19191)
                     /locus_tag="HTJ20_RS00100"
                     /old_locus_tag="pMCR-13EC-C962A_21"
     CDS             complement(18505..19191)
                     /locus_tag="HTJ20_RS00100"
                     /old_locus_tag="pMCR-13EC-C962A_21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            complement(19530..19679)
                     /locus_tag="HTJ20_RS00105"
     CDS             complement(19530..19679)
                     /locus_tag="HTJ20_RS00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            complement(19706..20800)
                     /locus_tag="HTJ20_RS00110"
                     /old_locus_tag="pMCR-13EC-C962A_23"
     CDS             complement(19706..20800)
                     /locus_tag="HTJ20_RS00110"
                     /old_locus_tag="pMCR-13EC-C962A_23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            complement(20812..21528)
                     /locus_tag="HTJ20_RS00115"
                     /old_locus_tag="pMCR-13EC-C962A_24"
     CDS             complement(20812..21528)
                     /locus_tag="HTJ20_RS00115"
                     /old_locus_tag="pMCR-13EC-C962A_24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000832283.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            complement(21540..24287)
                     /locus_tag="HTJ20_RS00120"
                     /old_locus_tag="pMCR-13EC-C962A_25"
     CDS             complement(21540..24287)
                     /locus_tag="HTJ20_RS00120"
                     /old_locus_tag="pMCR-13EC-C962A_25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(24306..24632)
                     /locus_tag="HTJ20_RS00125"
                     /old_locus_tag="pMCR-13EC-C962A_26"
     CDS             complement(24306..24632)
                     /locus_tag="HTJ20_RS00125"
                     /old_locus_tag="pMCR-13EC-C962A_26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(24646..25266)
                     /locus_tag="HTJ20_RS00130"
                     /old_locus_tag="pMCR-13EC-C962A_27"
     CDS             complement(24646..25266)
                     /locus_tag="HTJ20_RS00130"
                     /old_locus_tag="pMCR-13EC-C962A_27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(25622..25837)
                     /locus_tag="HTJ20_RS00135"
                     /old_locus_tag="pMCR-13EC-C962A_29"
     CDS             complement(25622..25837)
                     /locus_tag="HTJ20_RS00135"
                     /old_locus_tag="pMCR-13EC-C962A_29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(25834..26127)
                     /locus_tag="HTJ20_RS00140"
                     /old_locus_tag="pMCR-13EC-C962A_30"
     CDS             complement(25834..26127)
                     /locus_tag="HTJ20_RS00140"
                     /old_locus_tag="pMCR-13EC-C962A_30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(26204..27433)
                     /gene="taxC"
                     /locus_tag="HTJ20_RS00145"
                     /old_locus_tag="pMCR-13EC-C962A_31"
     CDS             complement(26204..27433)
                     /gene="taxC"
                     /locus_tag="HTJ20_RS00145"
                     /old_locus_tag="pMCR-13EC-C962A_31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(27437..27940)
                     /gene="taxA"
                     /locus_tag="HTJ20_RS00150"
                     /old_locus_tag="pMCR-13EC-C962A_32"
     CDS             complement(27437..27940)
                     /gene="taxA"
                     /locus_tag="HTJ20_RS00150"
                     /old_locus_tag="pMCR-13EC-C962A_32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            complement(28301..28648)
                     /locus_tag="HTJ20_RS00155"
                     /old_locus_tag="pMCR-13EC-C962A_33"
     CDS             complement(28301..28648)
                     /locus_tag="HTJ20_RS00155"
                     /old_locus_tag="pMCR-13EC-C962A_33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(28645..28893)
                     /locus_tag="HTJ20_RS00160"
                     /old_locus_tag="pMCR-13EC-C962A_34"
     CDS             complement(28645..28893)
                     /locus_tag="HTJ20_RS00160"
                     /old_locus_tag="pMCR-13EC-C962A_34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            complement(29198..29548)
                     /locus_tag="HTJ20_RS00165"
                     /old_locus_tag="pMCR-13EC-C962A_35"
     CDS             complement(29198..29548)
                     /locus_tag="HTJ20_RS00165"
                     /old_locus_tag="pMCR-13EC-C962A_35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(29639..29857)
                     /locus_tag="HTJ20_RS00170"
                     /old_locus_tag="pMCR-13EC-C962A_36"
     CDS             complement(29639..29857)
                     /locus_tag="HTJ20_RS00170"
                     /old_locus_tag="pMCR-13EC-C962A_36"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(30392..30619)
                     /locus_tag="HTJ20_RS00175"
                     /old_locus_tag="pMCR-13EC-C962A_38"
     CDS             complement(30392..30619)
                     /locus_tag="HTJ20_RS00175"
                     /old_locus_tag="pMCR-13EC-C962A_38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(30606..30857)
                     /locus_tag="HTJ20_RS00180"
                     /old_locus_tag="pMCR-13EC-C962A_39"
     CDS             complement(30606..30857)
                     /locus_tag="HTJ20_RS00180"
                     /old_locus_tag="pMCR-13EC-C962A_39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(30854..31396)
                     /locus_tag="HTJ20_RS00185"
                     /old_locus_tag="pMCR-13EC-C962A_40"
     CDS             complement(30854..31396)
                     /locus_tag="HTJ20_RS00185"
                     /old_locus_tag="pMCR-13EC-C962A_40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(31501..32016)
                     /locus_tag="HTJ20_RS00190"
     CDS             complement(31501..32016)
                     /locus_tag="HTJ20_RS00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            32541..32732
                     /locus_tag="HTJ20_RS00200"
                     /old_locus_tag="pMCR-13EC-C962A_43"
                     /pseudo=""
     CDS             32541..32732
                     /locus_tag="HTJ20_RS00200"
                     /old_locus_tag="pMCR-13EC-C962A_43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            32742..33941
                     /locus_tag="HTJ20_RS00205"
                     /old_locus_tag="pMCR-13EC-C962A_44"
     CDS             32742..33941
                     /locus_tag="HTJ20_RS00205"
                     /old_locus_tag="pMCR-13EC-C962A_44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000948429.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     ARG             complement(3997..5618)
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          858..1729
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(26208..27433)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13614..15472)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(17604..18508)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18509..19191)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(19734..20800)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.6999999999999997e-172"
ORIGIN
        1 ccctaaaacg ctctgaatcg cattttaagc gatttttttc atttcgcatg gttttcctca
       61 tggttgtatc gaacgaagcg gcagagcgcg attcagggct tcttagtgga agatccaaaa
      121 aggatcaaat aacggatcat atgatctgaa tctctctggt tttgagaaag gagataccgc
      181 tcgccgcagc cgaacggcag gagcgtagcg actgagtgag cgaggaagcg gaatttttgc
      241 gatgtgacat taggatcagt acgcacgaat attatattta tttcaataag ttaaagttaa
      301 ctagggacaa atttatggtc ttgaaaattg agatacataa ggcatcaaat atttttttga
      361 tgattttatg ttattgaaag ttatgagttt taatgattga tgatgtttgt tttagcttgc
      421 gggtaataat catcagggga cagatttatg gtaaggaacc atcatcgttg ctggttttgt
      481 tgagttatcc acagttcctg aatattcgca cgcattaaaa gccttatctt ttcttgtttt
      541 tcttaaaaac ttaaaaactt agagtagctc taatttattg attttaagcg tgttttttgt
      601 ctaaacatga gagcttcctc ggtgatatcc acaaaatgtg tcataagaat actaattcgt
      661 tgttctttct ggtgaaccta ttttgaatct gttgttaatc tgccttgaat ccagtgtgga
      721 ttttgagcat ggattacctt tgtgagataa atcacagata tgaaacatga gaagatactc
      781 ggttaaacat gatattgatt gtgttgtaat atcatgtttc tggtgttaaa gtctgattat
      841 caagagtgtt ggaacaatga gaatgacgac aaataagact tccctttctc gcttaacaaa
      901 agtgagacat cgaaatgaat tgaattcaac gctgtcaaca ttgcctatgg ctgctaaaaa
      961 agtcttattt ttagccatgt gccagataaa ctctaaaaat gaattcgatg atgatcacat
     1021 attttatgtg acagttgccg attacattaa atgggttcag gttaagcctg atgcggctta
     1081 tcttgctttg agggatggct ctaatatatt agatacgacg cttcttaagc tgaaacatga
     1141 tgaaatatta gaactgagta gtgatttggg atttaagttc actaaaagta atgtacctga
     1201 ttcaatgaat ttgagtctga ctgttttttc tacttattac aggaatgaag gtagagtggg
     1261 gatcaagttt acgaaagaag cgaaaagatt tttatgtaaa cttattggtg aagaaaaacg
     1321 ttatacaacg caagttttat tatctgttgt tcgactgaca agtgttaatg ctgcatcact
     1381 atatcaactc attcgtaaaa tatatagtaa taattctcgc gcgaatagtt ttgaaatgac
     1441 aatagatgaa cttaaagatg aattgaatct ttatacaata ggggcggggg gggtgaagga
     1501 ttataagtat ccagattatc ctgcttttaa aagagatgtt cttaataaat cagtaaaaga
     1561 aatcatgaaa catacggaag taaagaactt aagtttcgta gtttcagaaa aaattgggag
     1621 aaaagtttat aaattaaagt tctcttatac aattggttat gagggcgata ccagggaaga
     1681 ctcagagttt actaatatgt ttgataaaat gtacccacct gaaaattgag gtcataacaa
     1741 tatgaaggat aatatcgtag attttcaaag aatcagggac agcatgaatg atcattcaac
     1801 cgatgttgca aatgatgccg tttattctga caaaaagaaa atatccaggt tcttttatct
     1861 gctgacacct gtcagatacc ttctggctgc ttccctttat ctggttgttg ttattccatt
     1921 ggggattctt gcgggatttc gtttcttgtt gcagttttta ggcggtatcg cagtgatggg
     1981 tttaactatc gcctggtaca tgggtgcaga tgtcagtagt tacgttgttc ttgctgcatg
     2041 gatcgcctta actgtttgtg gttgcgcaga gcagattttt gaatggtgga tcgaaagccg
     2101 ttttgcgttc cgtctttttg gtatcgggag aacgccggaa gaatgagcgc cgatcttaca
     2161 gacagtaaag aacaatgcag gatagacaat ccgatgtcaa atgttaacat ttgaatcaga
     2221 ttgtccaaaa acgatgtttt tgcctgtgcc tgccgcaggc tcttttaaaa ggaatatgaa
     2281 atgatattta accgtaataa aaaacgagat gaacaacata attctgctgt tgttttgcca
     2341 tcagctgaaa atgtattctc tgattttatt aagaacaatc cagctttgac aaccaatgct
     2401 tttacagcca ttggggaggt tgattgtttt actaaagtac ccttactctc aaataatgaa
     2461 tcagagtttt taggcattct ttcacgtaat attatacctg attattccgt ttttcccaaa
     2521 atacggctca ttgaatttgt tcgtcctcat ggtagcgagg aagctgtaaa agatttaatc
     2581 cgtgaagtgc agaatataac ctgtgatttt gtcctgaaat catatgatga aacagtcctg
     2641 gctgttatcc agtttggtga aaccgcaacc gtcagacagc aaaagaaaaa actgattatc
     2701 cagcgcgtgt gtgctatgac aggaatttct tttttcgaat ttaaaaacac tgtcgatatg
     2761 atggggagcg aagatttttg tcagttcctg cgggatgatg aagagtaatt aaaaataagc
     2821 ccacatattt gcgggcttaa caggctcttt attagtactt gctgcatttg tgattactgg
     2881 caacacagtt ttcactgggc agtttttgcg gtcgctttta attatggtta gtggaactca
     2941 gacaggtaac tcgacctctt tacatattaa taattgatag ttttacctgg cttccttcaa
     3001 catttatttt gttgaggaat ttatttaaat acgcaatatc tggagtggaa ccgcatattt
     3061 tcatcaattc ttgaccgata ggcgtaagta aagccgttcc tattggtagt ttccaggttg
     3121 atgaatcatc gctttgaaac tcaactgaaa aaaccgttcc gtaataaccg atacgaccgt
     3181 ttttactttt aaagccgatt aaacttgttc acccttctta aaaaacaccc acgcaagccc
     3241 tgccgacatc gcccacgcaa gcatcatcga caccaccgta tggctcaaaa aatgatcgcc
     3301 aataatcatc ttatagcagc ccatcgccca gcccaatgcc atcaccacga tgatgatcgc
     3361 accacgacgg cggcgcaaac ttggcgcaaa cgcaaaggca aacagcgcaa atccgctact
     3421 ggcatgcgcc gcaggaaagc acttatcagg aatggtgtta cgcatactat caagcatcgg
     3481 caaatacggc agcgtaccat caaaaatcgt caaatgcaca gggcagacca catgagtata
     3541 tgccttcaat gacgcgacaa ctgtcggcac taggatcaaa gtcagcacca gatacgcaag
     3601 ctcacgcacg cgaaattttg tcactgggac aagccacttg gcagtgatag cattgccctt
     3661 tttctcatga taagcacggc aaatggtcgc aaccagcaag tagatcagca tcgccatcag
     3721 cagctttttt agtccagaat acagtagcaa atctggcaaa aaagcttgct tggcaaccat
     3781 ccaatgcccc atacccagat aaaaccaatc agcgaccatc agattaatct gactgtgctc
     3841 aagggtcagc gtggcaacga tcgccatgac aagagcgata ctcatctcag caagtaggcg
     3901 tttatttgat aaatacggca taacaaaccc caccccaccg cccataatac gaatggagtg
     3961 tgcggtgggt ttggaaaaaa tacagggaga aatcagcgga tgaatgcggt gcggtctttg
     4021 actttgtccg cggtgacatc aaacagcttt aataatgtcg gcgtgatcgc gtcatgggtc
     4081 aggacggtat cggttgccat tggcgtgatg ccagtttgct tatccgtcca gaaaaatgca
     4141 ggcacactgc gctgttcttt tggtgcaaag gcatttggca taccatgtag atagacaccg
     4201 ttctcaccca gactttcgcc atgatcgctg acatacagca ttgagacatc ataggcattg
     4261 ctgtgcgtct gcagccactg gatactttga gcgatgaaat catcggtggc aagcaaggca
     4321 ttgtcataag cattgatcaa ggactgatgt tcgcacttgg caagctcatt accttcacac
     4381 actggcgtga atttggcaaa cttttcatca tatcgcttaa aatacgcagg cccgtgattg
     4441 cccatttggt gcagcatgat cagcatatct ttgccgttat tggcagcgac aaagtcatct
     4501 aagccaacga gcataccgac atcgcggcat tcgttataag gattggtgtt gcagatggcg
     4561 ttgttggtcg cggatttata atcggcaaat tgcgcttttg gcagcttatc catcacgcct
     4621 tttgagtccg aattattatc acgccacaag atacttacgc ccaagcgatc cagcgtatcc
     4681 agcacatttt cttggtattt ggcggtatcg acatcatact catccgcgcc cagatagctg
     4741 aacatacacg gcacagaata cgccgtcgat gtgccgcacg atgtgacatt gctaaaattg
     4801 gtcacgccat cgatcttggc aagctgtggg aaagtatcgc gctcatagcc attgaagctg
     4861 acatgatcgg cgcgtgccgt ctcaccgacg acgaacacca ctaggcgtgg cttacgcata
     4921 tcaggcttgg ttgcttgtac cgcgtctttg gcgtgataaa tggtatcttt tggcgcactg
     4981 gcttttttat actcaatact ggcaagctta cccaccgagt agattggcat gatcggattg
     5041 acatagctac gcagcggctt atgcacgcga aagaaactgg cataatgact gctgaacgcc
     5101 accacaggca gtaaaatcag cgcaagactt gccacgatca agcccaatcg gcgcatcaaa
     5161 cccttgcccc aagtcggata atccacctta acaaaagcca caagcaaact tggtagcaca
     5221 cccaaaccaa tgatacgcat gataaacgct gcgtttaata gatccttggt ctcggcttgg
     5281 tcggtctgta gggcattttg gagcatggtc gtatcataga ccgtgccata agtgtcagta
     5341 aaataactgg tcaccgcgcc catgattaat agcaaaatca acacaggctt tagcacatag
     5401 cgatacgatg ataacagcgt ggtgatcagt agcatcgcgc caaagagcac gacagcgatc
     5461 gtcagcacaa agccgagatt gtccgcgatg ggataggttt ggctgatttt atcaaaaaag
     5521 gtaagattgg cggtcgcggt caagaaaacg gcaacactcg ccacaagaac aaacggactg
     5581 accgagcgtc ggtaccacac agaagtatgc tgcatcatga gaaactactc aaaaaataaa
     5641 cggtgggata attgcggcaa tcatactgca attttttaag aaatccagta caatttatct
     5701 gatataaaaa attatgcgat acagaaatat ttcagaaatt ttattaggca atcattgcgc
     5761 aatcccatac tgaatataca cttgagagta tttataattt gtatcttaaa ctataaccaa
     5821 tgccttgttg aataacgcct aaggagtcta aaaccgaaag tttatcgaaa gttatacctt
     5881 cagtatctcc attctcatcg gtaaataaaa ttggagtgag tttgtttgcc tgccaaacaa
     5941 actttccaaa ataagtaata aggttcgctt cttctttgct tatagtggat aatagtttta
     6001 aggttctacg actaaacgaa ccttttgact ttgcttcttc ggataaaatt cttccccaaa
     6061 gcatttgcat ttgttcgtca ctaatatcct cacactccct gaagaaagcc tctatccaat
     6121 cttcgtcaat atcagaaaca ttatcactct cagatagact ttgtgctgct tgcattgtta
     6181 tattttcaat gttttcctgt cgtttcgttt cacgtttgag aaaacgctca actgcccgtt
     6241 tttctattcc ctcaagctct aacctagaaa ttagctctgt gcgtttagct tcagcttcag
     6301 ccttcgcttt tttcctgatt cttgtaggtt cataaagcac accaatagca tcacttattt
     6361 tttcaataag ttttgttcct ggttctgata atcctgataa acttaaatcg ataaggctca
     6421 tctagtgctc ctaaataccc gtgcaacaac tgtacaaggg tattttattg caagagtaac
     6481 taaaaatgaa tcacaactac atttaaaagt gatgaagtta gtacattcag ataagtatct
     6541 aaaagaaaat gataaagagt ttattgggcc acttgctatg attaatcatt tctgtcagct
     6601 ctgaaccaca aattaatgag ttcgttttcg tccagatcct gataagccag taatgctgct
     6661 ttgattattt ttgtcttggg acgatgcagt ctttttgatt cgttatcaat gaattcaagc
     6721 aattcattcg gtatgcggaa tgaagccagt tttgttttgt tctctgtggg gcgttttcct
     6781 gcgtcctgaa caaattttgt ggcgttcact tcctggtttt gttctagttc tggtagttcc
     6841 agctttgcca tgatgtacct tctaatctga ttgtttcaca ctaatgtata tcatgatgat
     6901 gtacattagt gtatgtcact atgatttaca gttccagttc tttcagcaag agttcgattt
     6961 gtgcttttgc ctggctggca ccgctaacat tactttttaa ctcgtgaacc cccattccct
     7021 gattcattgc tcttacaaag gcccggagaa aagaaaccgt gcttttcatt ggtttaagcc
     7081 agtaatcatg gctgttgaga gagtctctta attcttgttg ttcactgaac gtatttggct
     7141 ggcatttata caggagcaca tgtgcagcca gcctgggatt tattttttgc gcatccctga
     7201 caatttttgt cacagtctga agactgtctg cttccacctg ggcaagcggg tcgatcggga
     7261 caatcacaat gtctgcaact tttagtgcag ttctgaactc cgtgctgtca tatccggcag
     7321 tatctataat gaggatttct gctacatctg ccatttttgt tattttggca ctgatgtcgc
     7381 cataggcttc atttaatgcg acgggcggta atccggcctg ttctctgcgt ttattccatt
     7441 ttttggtcga gccattcagg tctgtttcaa caagccccgt tctctttccc tgttgtgaga
     7501 ggcaaacagt taagttggtt gccagatgtg atttacctgt accgccttta tcaccaccga
     7561 ttacgataat catagtaccc cttgtatgtc acagtgattt actcatgtgt atttctatgt
     7621 gatgtacgca tgcgtatgtc acatggacat actatagtgt atgtcacaat gaattacaat
     7681 gatgtacatc atgaagaaat acattattgt atgtcagtgt gatgtacccg ttggttggtc
     7741 acttgaaagc aaaagtgaca gcgttacttt cggtatcctc ccttgctgcg aaaccttgtg
     7801 gcaatacagc aatgagtatg tttagttaca acattcaaaa ttaagcgatt agattccgcc
     7861 cgtacgggcg gggcgcgcca ctttctgtag tggggagggg cagggaggtt atgtttgctg
     7921 gatgagcgga ggatggaatg agctattaga atgttgtaac taaagggtat cccaattaaa
     7981 ggccacttac gcggcctttg tctgtcggtt cttcggtctg gtgacatacc acgataaaca
     8041 tcccacagca acactcataa gcattccgga taagcgatca tcgatgccag gaaacaggca
     8101 accgacaaac atcgcaacag ggaaaggaag aagtaaacag cgccccaaaa tgctcataaa
     8161 atccataaat cctccttttt tgttaaaggg caggaagcgg ttcacttcct gcccttagtt
     8221 acgtttccgt tgtcatcaat cgatgaattg gcggatcagt tccagaactg caatcagcgc
     8281 gtttgcaacg gcgaccacag ctctgagaac gatgattaac atattaatca tccgtttctc
     8341 cagtaaaact ggaggaaatc accgttgctt gctcatacac tgcctgtcac aacggctctc
     8401 tcgcattgtt aggtgtagag tgacctcctc ctgccagagc accgaataca gcaccacctg
     8461 tttatccagc caggacttta aaatcgttca tacccgcaga tacgggaaca gcaggattgc
     8521 aagccaacta catgactaac tgactgatta tacgctacac aaaacacccg ttacaccact
     8581 aaatgtggtt ccttaaccac atttagttgc tttacagcac catagtgaat aaaatgtaaa
     8641 cgttaggtgt agagtaacct tctccggtca gagccaccaa ctcaagactg gaaaacgcaa
     8701 aaaagcccga ttaccagtcg ggtttttttg tgcctgtcgt tcggctgtag ttccgttttt
     8761 tctccttcat ttgagggatt tttctttcat tttttttgtt gcttccaata ctgcctgtcg
     8821 cgttggccaa cagcttgacg cgttaccggc attttcatta ctcaatattt ttcagtgaat
     8881 taaaatgcaa cagcgacaga aaaggacatt cagcttttta gatgaatgct atagcaacgg
     8941 tgtgctacgt aaaatctgcg aaaaaagcag gggtagggta ggttattttt cacgtagcaa
     9001 cgccatctgg cactgtacat aggtatgtca cacagacata cctatgtgta tgtctgtgtg
     9061 aggcactgtt gcaaagttag cgatgaggca gccttttgtc ttattcaaag gccttacatt
     9121 cgactatttg caacagtgcc ctgtgtgatg tatacttggc gggttattct gcctgttccg
     9181 gcttagtttt ctcaatgagg aaatcttcca tgaaatgccc ctcatcaagc agcttctgta
     9241 aagcaagcgg acgcttgcca ttaccagacc attctctgat ctcgccattt tcatccttaa
     9301 agcggtattt agcctttctt actccgcctt tggctttccg cctggttttt actgtatctg
     9361 ctgttacagg agcaaggagt gattcgggat caagacctaa agatatgatg tattcaatgg
     9421 cctgctgacg tttggcttca agttcctggc gttcagccgc ttcacgttca gcatcttcac
     9481 gacgttcttc aataacaacg ttgagctttt cctgcatttc gagcaactgc tcaaaatcaa
     9541 tctcacgggc aaaaacacgc aatgaacgaa tattcatcat agtacggctg ataatgccgt
     9601 attcatcttc tttagtcagt tcgctcatat aattaatcct cttatttagt gtacgtactt
     9661 ccagacgccg gaaggcacct tatcataaag tttattcatc accagttctg ctcggcgatg
     9721 atctgcggca gaataaaatg cctctgattc tgataaaggc agtttgtaac ggttaatctc
     9781 aataactttt tcaagagtat ctcttgatgt acattttctt aactgaaaaa gccattcctg
     9841 ctttgttttc acttcttagc ccttgccgga aaacccagct tccctgtttt cttatcctca
     9901 aggacaagaa ctgtatcata cgttccacca cttttctttt taaatccctt aatcaaatcg
     9961 gttttccctg atttaaccag tttttcggcc tgtgcctggg tgattttctt accagaaaac
    10021 tcactccaga ttttaaattc acatccggta cagaaataac ctttcggcct gataacaata
    10081 tgtttaccac aggaagggca gggagagtcc aggcgttgaa actgccctgc tggcgcagcg
    10141 ggttcaatct tcatctcgcc caggtcaaca tcagaaatca tgcctgtcag ttcaccgtac
    10201 agatcattaa taaactgttc aaccgtcagg tcgccatttt ctatggcagc ctgcttttca
    10261 gaccataatg ctgtcatatc aggattaacc gctataccag gcagggcatc aataagcgca
    10321 tatccggtat cagtaggaat aagtttccct ttttccagcg tgatatagtt tcttttcttc
    10381 agcgtttcca gaatggctgc gcgggtagct ggcgtaccaa tgccaccatg ttcatctttt
    10441 ttgtctttat ccttatcctt caacaatttt ttaattgttg gatcagtgac aaaatccgcg
    10501 acacgaacaa gcgcagcaag caaggaggct tcggtgaata acggcggcgg tgttgttttc
    10561 ttctcattaa caataacttc ttttgtcgtc agtgtttctc ctgtgcgaat tttacagagc
    10621 agttcaaaag cggaatcatc attatcttct gattcacctt cgtctgtggt ttccgcgcca
    10681 agaaacgcct caaatccgct gtcagttgtt tttctggcac gggcatagaa cgactcatca
    10741 ccacactgaa tggcaaccga tacttcctgg tatgcttttt caggcatgaa ctgaacaaga
    10801 tagtgttgtg cgatcgccag gtaaacattg cgctcgtcgg tgctgagtgc gttaacatca
    10861 ggcacactgg cggtcgggat tatcgcagta tgcgcagtca cctttgcact gttaaacgct
    10921 ttgcttttac gtgcagaatc aatatccagc gactgaggaa agactgattt cagggcatcg
    10981 ataacctgcg gcgcttcgct gaattgttca tctgaaagat atgagcagtc agagcggtta
    11041 tacgtaattg ctttatattt ttcgcgtagt tgttgcgtaa tatccagcgt tttttgtgcc
    11101 gtcattttaa acttcttgtt catgtattgc tggagtctga ccagattaaa cggcaacggc
    11161 gcagccgttt ttttatcatc agttgctgct gcttcaactg tagccggttt tcctgcaagg
    11221 gatgcagccg ttccgtctgc ccacgcttta tcaagtaatt tacggtctgt cagcggagca
    11281 aattcccctg gcttccagtt cgccctgata acatcagcac cacgctgaaa gactccggtc
    11341 atggtgtagt aaaaactgga tttatggttc tggttagcac gggtacgatt cacaatcagg
    11401 ccaagaacgg gagtctggac gcgcccgaca gacagaacgc cctgatatcc tctggctttt
    11461 gccggaatgg tgtacgcacg tgtcatagag aagccataga cggcatcggc aactgaccgc
    11521 gccaacgcct taaggtaaag tcctttgaaa tcacgattgt ctttaagatt tgccagtgcc
    11581 tttttcactg ccggaagcgt gttgtcgtta atcagaacgc gctttacggg ttttgtgttg
    11641 cctgcatatt ccaggacttc atcaaccagt agctgtccct catcatcagg atcgccagcg
    11701 tgaacaattt cagtcacgtc tccacgtctg ataagttcga gaatcgtttt aacctgtttt
    11761 gctgcacttt caacaggctg atacttcacg ggataaagac gtaaaggaag cgtttcaacc
    11821 ttccaggctt tgtattcagg attatagttt tccggcggtt gtgattcgat aatatgacca
    11881 aaacagttag tcacaatggc gttatcactt tcaaaccagc catcatggcg ggtaaaattg
    11941 ccaccaagtg ccttaacaat atcatttgct actgcgggtt tttctgcgat aaaaagtctc
    12001 attttgttat cctttacatt gaattaatct tttggcacaa cggatagcgc gttcttccga
    12061 tactgtatgt tgccagttat ctttacttac tactctgaat ctgcgctcac agccattaaa
    12121 ggcaaaaatt ccctcacggt taagtatatt agttgcaata tcccactctt taattctcaa
    12181 aaaggctgtt acagcaaaac cagaatacga agcaattttt gaaatgatgc attcatcagc
    12241 ttttattaac gcggcaaaag gaactatctt ttgatgacca cacgaaatca gagggagtgt
    12301 gtgaatatca taataagcta ttgaatgaca atccatgcgg gtatcatcaa tagctgccag
    12361 ccagaatcct gcctggtctt ttatttttct gctaatatct gaatctgaaa caccagggtt
    12421 taataaatat ccaccataac agatatggaa ggggtgagca gattcatcaa tatcaggaag
    12481 ttcttttacc ggattttctt tccagtcctt cagaacaccg aattcaagag catccagcaa
    12541 aaactttcgt tcaaatccat caaacaatgc atattgcatg tgttaatcct ccaggtaact
    12601 taaatagtca ggttgtgccc cgttcttttt ataaagttct tccctttcct ctatatcttc
    12661 atctgcttcc tgaacgctca gatcgagggt tcctttcaaa aaggcacgag cattttttat
    12721 tacatccact tatgcactac cagcagataa gcacaataaa atagctataa tagatttggt
    12781 tactttcatt tctaacctca gtgattgtaa ctaatcatca catttcgttg gcgcgaaatg
    12841 tgatacttgc agtaccctga tacccgttgg cgcggttatc aggttttaat taacctctga
    12901 cacgaccaaa cttactcatg atttttgata tagcagcagg atcagccgtt gaacactgat
    12961 tcagaaatgc tttacgggca tcaaaagttt tactccagcg aatgcttcct tttttctttt
    13021 tcagaatatt aaaaaaatcc ttttcagcac ttctgcattc actccctcca ccttgcccga
    13081 ctgcttttcc atataagcaa agtaccgaag cgcaagggtc agatgcatta gcatttgttg
    13141 atattaatcc taacgccagc aaggctggta aaaccactct attttttcca gtcatttttc
    13201 acctcatttt gtgctttaat gaaaactatt tttacctgtt caggtaaaaa acttttctcg
    13261 tttcgccatt ccacgactgc tgttgcccct tccatcttca acagatctct gattcgaaac
    13321 cacagcccag catcatgtgt tgtcactaca atcctgtccg catgagccag agcgtaataa
    13381 aacgtcggcg ttctggcctg tactccggat aaggaagtga tctcataaac ccatccttta
    13441 ccgctaacgt ctgactttat gacaccgtga aaatcgggga catatggcaa tgagggattg
    13501 atcacttcat ttgccttttc aaactgcacg ggtgttggct ctggcggtga tgaacaacca
    13561 gcaagaatgg aaatcaataa aaacactaac ttcttcatca tgttctcctt tatgccgctt
    13621 tatcgagatc aggaacatct tttactacca gatacccttt tgcgacgatt tcattttcaa
    13681 atactttctg tggaggcata accagctttc ctttcttcct ggggaaaatg tttgcaagtt
    13741 ccggagcaac cttcacaaag tcaccaaaga aatactcatt gagatagtag aatgcctttt
    13801 cacaacgaat cgggttttct cccttcattg ccaggatttc ttcactaaat ttcagtgtaa
    13861 gtagttcatt tgttgttacc agtggacgct caataaggct ttctgattca gatctcgatg
    13921 ttcctccacg actacgactt ttgcttttcg aaccgatagt tactgttcgt gtccccatcg
    13981 cttctgataa ctcccgcgca tctttaacat cagaagcgga ataaacaatt ttgcagggtg
    14041 cacttgccag taatgtttta ctgcctgcat aactgtagat ttcctcaagc tgagagccga
    14101 cctggtaaac aatcaacaat ttcagcttgt aaccagcaat gagaccagat ttactcttga
    14161 tgtaatctac agcaccaatt gacgggaatt catccagtag aaaaaggcag tcatgcttta
    14221 tttccggatc atccgttggc atcttttcaa gattcacctg tatagcaagc tgaaagaaaa
    14281 tatttgttat tttttcagcc aacctggcat tatcaccaga aatacagaaa taaattgtca
    14341 tccgcttttt ccgcacctgc cgcaaatcaa aatcatttcc atcagtagca tttgccacat
    14401 tggggttagc aaacggtgat aatccattaa gaaagttgct tttgagttgt gcttttgttt
    14461 tatcctccat ctcaatgaat gccttaaggc cagcgacacc aatatcagca agcgttttgt
    14521 ctttccccct taattctgaa cgaatatcat tcatgaatga aagaagatgt tcgggttctg
    14581 cacgctcaaa aagctgatta attttagaca ttgagaagac aggctcaata tcaagcgttt
    14641 ttagtccagc cttatcattc ttaatcataa accataaagc gacagcataa gatttaaaaa
    14701 caccacccgc cgtcttatta agatagctac cagcatccgc accatctgtc ttataggggt
    14761 aaataatcaa cgaaagattt tcaagctgat taaaaccttc actcgttccc atgctgacat
    14821 aatacaaagg gttaaagcgg tgcgttaccg aactaaaggg atcaaattta taaacctcat
    14881 gacctaaaat caccttcctg ataagactgg tgatattaaa acactccctt tttggatcga
    14941 gaataataac actccaccag tacttcataa gattagtgat gactatacca gcacctttac
    15001 cagcccttgt tccggctccc atagcactga atgctgctcc cacatagcgt atgattttct
    15061 tgtcttttat accaacgata atcccgtctt tttcgtcacc gtcaagcgtg aaactgttag
    15121 actcacgaat atcattaatt gttgcaaatc gcgcatcacc gaaaatattt tctttcttct
    15181 cgttcagttt tatcaacgcc gcaacaggga tgataagaga cacgacaaat cctgctccaa
    15241 gtgcaacaaa ggctaaaact ctgatatccg tagtcacaac agataaagta aggctgtctg
    15301 taaaaatatc catcctgtac gcatcaatgg atctgaacac agccttcaat ggtttcagat
    15361 tctttatctg gctgaagtaa agcacaaaga aaataaatga acctgcaaac catgccgcaa
    15421 caagcataac agcgaatata aaaacagatt gcgacttatt aaaagttttc acattcagcc
    15481 cctttgtaat agatgtcgct catatgcctg acaacaccat ccctgcctac atgaacaaca
    15541 atatcaatac tgtccataat gattttacga agaacatcaa aaggaagatt ctgacattct
    15601 ctgttctgat aacattttgt caccatctga atgatggcat cttttgccga ggccgcgtga
    15661 atggacgtca tgcttccact gtgaccagaa ccagcaactt tcacaaaatc ccaggcatcg
    15721 ccacctttaa tttccgccag taaaattctg tcaggtttca tcctcaaaca ggatttgatt
    15781 aatttcgctg ctgttatgat agagccggaa tcactgccgg attccgaagg gtaaaaaaga
    15841 tgcacataat ttttatggat aaaaaatttg atttcctctg catcttcaat cgtaatcagc
    15901 cgtgtattca atggtatgaa atcgataaga ctcttcatat aagttgtttt accggaaccc
    15961 gttgctccgg caaatacaat ggttttccct gctgctacgg caagctccat aaaagcagcg
    16021 atatttctcg ctctgaataa atcaagaagc tcatcatcgt atgtcctgtg tttttttcct
    16081 gactggacat aatcataaaa accgttcgcc acatattctt tatgtgttat ctgccgtgtc
    16141 gatggtttac gtatagtgat tgaaatagta tttctctcac atgcaggagg aaaaacgacc
    16201 tgtacacgtt ctcctgattc cagtgttgct gaaagtaatg gcttggtctc gccgatttca
    16261 tctccccgaa atgatgccag cgtatgagaa aaacgtttac aaaaatcaaa ggtcacactg
    16321 tcattattat ggaatgtcca gtcaccatta atttccgtcc agatttcacc aggacggttg
    16381 acagcaattt ctgttagccc ttcaatatca agaaaagact ggaatacttg atttttataa
    16441 aaatcgagcg atttattgct aatgctgaca ggaataacgc cactcatact attttacctt
    16501 taactcatag atatcagaaa aatcaatgtc ttccccgacc ataatagtga ttatgtcacc
    16561 ctggttttta tacattgttg gaggaatatt tatcgaattc tcaagtgcga ctttagccat
    16621 ttcagccatt gcctgtcgtg agttttcggt ataatccaca ttacgatctt taccaggtgc
    16681 attatccgcc gccgccgccg ctacatcctg aaccatactg agcataagag aaccaccaaa
    16741 acgctcccag aagtgagaat cgatccatcc atcaatgccc gcttctccca ggggaccagc
    16801 tgctgctgta tttaccatat cgattcgttt attatcaggc gtccgcaact gctcccacat
    16861 aacgaacatt ctgccctgtc catgattcag cgttccggtc tggtaacgac caaaagcctt
    16921 agttccctgt tccagtaatt ttgtgtggtg attagcactc catacatctt ctgtaaaaac
    16981 acaactgata cgacctgcaa catcagaaac aaagcgtgtg gttaaagcac acggtataag
    17041 cctgttttcc tcaatataga gatcaggatc gagattaatc gcagttactt tcattcctga
    17101 tttaccaggc acattttctt ttgattcttt cctttcttcc tgtggctctg atgtctgcga
    17161 tgtcctgctg ctgccactcg cgccagtctg actccctccc tgaccagcaa caggtattga
    17221 caaatatttc ctgaacccct cctgtggcac ttctttatga ccgtcagaaa cagtattacc
    17281 agaaccagta gcgcctggat tgataattgc tttgttctca acagggtcaa aattctgacc
    17341 aagaccagta cggattttac ttacctgaga aatgctggta tcgcctgttg attctgatgc
    17401 tttctgctgg tcatctgcaa aataattttt atatgttttc agcgcaaaaa taccaataac
    17461 taatacagcc atcagaccaa acagaaagcc agaacgcata cgattatttt tgttcctgat
    17521 atcaccgata tcatgaacgg gtttactttc cagctttgac tgattcgcat catcaactaa
    17581 atcatctgtt tttttcatat cagtcattat tcacctcaac acgttctacc gcaggagaag
    17641 atgtattcct ctggtcggtt aatacttttc cgaaagattt attcacaaca ccaacaacgc
    17701 tgtttccata gcgtaaaaca aatttgtcat taacgttgtg gataaccata attttatact
    17761 tgccttttgt ttccacatta aaagcaagtg tctgttcctg accatctctt acagcaaatg
    17821 gagctgggaa tactttccct gagttaaaac ctaaaaaggt aaatatgcca ttatcataag
    17881 caaaatcagg ggcgatacga cgactgtcat atttttcatt gactcgcatg aaataattcc
    17941 agtttttagg cgcatcggct ttttcaaaac tttcagaaat cagtttcttc tgtctgagtt
    18001 tttcctgttt ttcttttgcc agccttactt cttctgcatt ctttttcgct atttctgatg
    18061 gatagcggaa ttgtacaaca taagcaggtt gcgcttgttt ttcttctgac agaaggatta
    18121 gatccagact gtaaatccgc tttgttgttc tgacaaaaag attcgtatcc cattcctttt
    18181 ctgttggctg gaaaaccttt ttcagttttt cgccttcatc gccttcctgt tcctgaacaa
    18241 cgggacgcgg attgatgtaa acaacattat catcggttgt gatttcccat ccggcaggaa
    18301 aaccagcctt tgcactgata acagcctcac cttcatcaaa aacaactgat gtgagaaatc
    18361 ctgtctttga ccggataacg gtcgtattcg caccgttata actcacagtc tgcatccttg
    18421 catcaaatcc agaaggtaac ggagtcgctg ctgaatacac aagcccagga agcaataaac
    18481 atgtagcaat aaataatttc ttcatcattc ccccctgact tcccgatctg tctggtaact
    18541 ggttacagta aaaccgagag gattaagctc acgactggaa cttttaaccg acttttctgg
    18601 ttcaaaacga tatgtcacac gagccgtcca gtatgaaact gaaacctgtc cggtcgtgaa
    18661 gtttctcgtc gtttttttaa agcgcacaga ggccagttta tctggcgcgg ttgcgtcaga
    18721 aataatattg gagatgacct caaccgcaac gtaagaaccg ttattatgat aaattttatc
    18781 aggagccata tcgctgttat acagacgaag ataatcgtcc tttactgtat tcgagctata
    18841 tagctgtaca gtctcataat catactgaag agacggataa ttataacctt cacgtaaagt
    18901 aatatacgtg tttacaaaat accgcccaag tgcttcagat tgcaggatct tttcctgttt
    18961 aacctgagta atcagttctg tacggcctgt tttttcgcca acagccacaa tagcaagctc
    19021 tttttctttt aatggcacaa gaacacaaat tgcagcccca agacataagg acagtaatga
    19081 tgctgccact gcaacacgcc atgcattttt cttagaccgt tcattttctg catagagttg
    19141 tctttcgaag gatttagctg tctcaatctt cttttcgatt ttttcactca taactgatta
    19201 cctggctgtg gttaatgggt tccagttcgc cggaaacatc agaaggaagg ttattccttg
    19261 tggtacatcc ggaaatctga aacaggaaaa acaaaagaat cacacatcgc ataaattcac
    19321 cttattaagc gttcacgaac acctttaaga gataaaggcc aagatgaaga aaaagaatcc
    19381 caccaataag aagcaaaaag cgaagatcca taccctttct ccatttcccc gcaagcccta
    19441 aaccaagtct tattcctgcg aagcaggtaa acactcctaa aagaaggatt aggacaaaat
    19501 aaagtatcat gacaaaataa tgaagcatgt cacaaccagc tctgactgat tccacctaag
    19561 accttaaata aggtaattcc cgtgattaaa ataacaagct gggttattag ctttctcttc
    19621 ctgctccatc cggaggtgat atgtttatca acgaacacca gtatccagtt cagggacatc
    19681 acataaaaaa aacgtttcat tattttcatg cggcgttcct cgctcgtgct ttttccaggg
    19741 atgcaagtct tctcgccttc atccccaaag cacctgtcgg agccagcgcc tgatatccct
    19801 tttcacccgc ccattcagcg gctgttcgcg caccacgccc catcagattc cctgctttcc
    19861 ctgataaaga attcatgccc gttcttccgg ccatccctaa gccaaaacct aaaccagcac
    19921 gaccgccaag catcagagac ttaccagtag ccataccagc agcacccagc cccatcatgg
    19981 ctgcgccctg aacagcacct tcaacacctg cacctgctaa ctggacagca aaacctttag
    20041 ctataagaac caataaagcc cccaaaaacc cagccataaa gcccatagca cctgtagata
    20101 caagatttac attccctgtt tgtgcagcac gaatagcatg ggaaaggatc atcccttgaa
    20161 aatccattgc tatccgaatc actaaactgg caaatagaac tgtcagaata ctggagaata
    20221 acgattgtaa ccaattatta aacatgctcc gaataaaacc aaacatgaga caaaagataa
    20281 atatcggtgc tgtaataatc aggagtttca tcgttacatc tgctgttaaa aaaacaacca
    20341 cactaacagc cattaaaata agcgagccag tccatattaa aaactgacca actacacctt
    20401 gatctttaac atatgttgat ttatctaaag agtatatttc cgcaccaaga ttttgcgtac
    20461 ttttccataa gttatctaaa gtttgccaga cactcacccc tccagaaaaa ccatctttca
    20521 tgccttgcaa cgcatcagta gccgcagtta aatacccatc cgcattagtt ataaacatga
    20581 ttatgattgc aaattttgat agatcccata ctaaatcctg caagggcgtc tgagttttac
    20641 tggcaagtat ttgatatcct ttccatagta catataacgt aaccgatacc gttgctaacg
    20701 acataatatc attagcaaat tgtgtttgat aactggagct gattttatct accgaatcca
    20761 taacggtaga attatattta acgaaaaacc cctgagccat atatccacct tttaatcaaa
    20821 agttaatggt tcagcttctt tctgctgttt aataaaggcc tcttgttctt gtgtgaccaa
    20881 catctcatga tctgctttat ttttattatt ccagacatca tactgcattt ttatcgcctg
    20941 catttttaac gactgcgcct gtaatgcgtt agccaaatcc tgggattctt tgatatcctt
    21001 tgaagcctca attcggctac ttaaactctg aatctgagac attgcacttg ataactgctt
    21061 attgatctca tttccctgtt caatactggc agccgttgac acaatccgtg ctttacagag
    21121 attatcatta cgttcatagc ccgtattaca catatcgaag gctccatact tgctgaacag
    21181 atttttagcc tggctgctca atgcaccttc gggattactg gtaaaatcag aaatataact
    21241 gttcccctgc ttataaatat ttgtcagttc tgattgtaga ttacttagtt catttgtgaa
    21301 tgccgcaata ttccttatac ctgttgccgt ggctaactgg tctttatatg cctgaatctg
    21361 actctgataa tgttgcgcag tttcggacca ttgtttgagt cgttctgtcc atcttgcggc
    21421 ttcaacagcc cattccggac tggcatcaat agcaaccgga attcctgcgc tactataaaa
    21481 tgaagccgtc aataatgata tcagcatcaa tgtttttaca cgtttcataa attcacctgt
    21541 cagattgccc gttcgagata ttcaggaacc cagtcatcag gctccattcc ttcatgataa
    21601 atactttcaa aaatttccag attatctgca ctggcagaca atattttcag atatccacca
    21661 agtcccgata aatcgagtgt cgcaagtgct gaaaatgatt taccatcccc ctttttaaga
    21721 ctgctttttt taatcaggaa ctgtctcgac attggatcaa gatttttaac aatattaaat
    21781 tcttctggtg tcagcttaag cccttccaca taatcattgt aatcggcatc agggttagcc
    21841 agataaatac tggtgctgca tacttcaacc acagcccgtg aaatcttatt cttcagtatt
    21901 tcatccggag actgagttgc cggaataacc agcccgttca gcttacgaat tgttttcagc
    21961 ttgttataga caaagtcgct gaatgcttca tcctgtagcc atttccagaa ctcatccagg
    22021 aagataacaa gacgccgacc gtccagaagc tgcgtaatgc gatacagcag atagaaagta
    22081 attggtgcac ataccatcgg atcatcaaga aactcggtgc catcaatgcc aaaattattg
    22141 acatgctgaa tattgaatgt gtctttagca ttatcaaaaa cccagccatg agcctgacca
    22201 ttagcccatt gggacaaacg aaggataatc ccgttctcct gctcatcccg atcatctctc
    22261 tgcataagat gctcaagcat tctggtaatg ccatattccc gcatttcgcc aggaggaaaa
    22321 tccatcacag catcaacact ttcgctaata agcaatcttt ctcttgtcga taatctttca
    22381 ccatttctgg tacacagaat ctccatcagt tgcttgacaa aaatacggtt acgttttgtt
    22441 gcctccagag caaaaggatt ccatcctgtc ggctcaccgc tttttacggt gtaatattcc
    22501 ccaccaagca ttcggataca taattcagcc cctctgtcct tatcaagaaa gacacacgtt
    22561 aacttcttat tttttgcaga atcagcaaaa gtttcggggt tattgtattt ctgtaatgaa
    22621 caggcaagat atgacagaaa cattgtctta cctgaacccg ccgttcctat aactttagta
    22681 ttagcaagat tcttttcacc tgtttcatct ttaaacagta ctgagttatg taaattcaga
    22741 taatacgcct gtttgcttgg tgttttgaga atagcaaccg cttctgtcca gcagttttta
    22801 tcgcgtttcc cctggtagaa attatggaat gatgccagtt caacaaaatt tacattcgat
    22861 accggagata aacgcggcct taaatgaaaa accgccggaa gttgggcaaa atatgccgct
    22921 ggcaatgaca tggttgacaa tgccggaata atgcccaggt cagtaaagtc tgccgtaatt
    22981 ttacttacag attcgtcaag ctccctgata ctgtccgcca ttaccattag cgtgaaatgg
    23041 tagtaaccaa aggatatatc accggacaca atatcatttt ttgccttttc cagttcctgc
    23101 aattgagtaa ccgcatcatc ttctgtactc tttaactgtt tttccgcacg tttaatggta
    23161 ctcaatgctt ctgatttgct catggatgta tatgaatgcg taataatata gtcagcatca
    23221 gaatactgga gcacatcaaa aacaccggat gcagtctcac tacagaaatc tttaatttca
    23281 atagaacgaa agaatttatt tccatcaatg cggcatatct gtccggtatc atgattaaaa
    23341 aatacatcca ctccaccaag aacgttatac accggagaat cagtgactct gattttttgc
    23401 aacttccctg tcagaagata attatagaat gacagttgag aagaaaatac ctttccgttc
    23461 tcctcataca taccaagaag tttacaggta aatttatcaa gagcgccaga gaatgtatta
    23521 atcatttcat tcattctctt cacatgaatg ttaatatcat ctttcttttc tttaatactt
    23581 tttttgcgtt tttccagttt ttctacacgc ccatctggtc tgtagatcag cgtaaagtaa
    23641 agagtgttcc ccttgaattt atttttcttc atcgaaccat agtaacaatc tgatattata
    23701 tcagcatatt tattacctga ttttgtggta aaggcatcat aaaaactcgc cctgcatgaa
    23761 tggttataaa atgaaacggg cagtccggaa aatgaacgaa caagagtggc aacctgtgag
    23821 tcaatagttt ctaacatctc cgcagagatt gactcaaagt taatccccat cagttgccat
    23881 gtcgctacat aatcaccgtt ctttgttttg acaatatttt tatcaacatg gcttgaatag
    23941 ggtatatatt taccagtagt gattcgctca ttcagtttca tggcattaac aaactcatca
    24001 atttcaacag cttcatattg tccggagagg aacgctcgtg cgccaaaaaa acgattacac
    24061 actgagtttc cgagcatttt cagcttaaga aagtaaaggt taaaaatgtg gtcgtcctgt
    24121 ttagtgatca ttctgagaac aaaaaccgcg acaggaatag ccagccagat cagcttgctg
    24181 atataaaccc ccgccagaaa taacgcccct gcaaccacag taagagggac gacaggaaca
    24241 cccacataca tagcagggcg tgtcatcgct ttataaagtg tagacatact atcgtccccg
    24301 ctttattagt taaccatcat tgatgcaact tcagccgcac tggcgataac aattgccccg
    24361 atgatgatag gtgagcattc acgaatcgtg cttccaccaa acagaacctt gtagccgacc
    24421 cacagacagg caatagtcac cgtgaccagt gaaaggccac taagccctgt tttaaccttt
    24481 tccagcagtg tttccgcttt tgaaaatcca tccgcaaagg ccggtgatgc aactgttgcc
    24541 ccggcgactg ccataactac cttattttta cgctgcatta cagcagtgac agatgaactg
    24601 atacgattac atactgattt cttaaatgac attttataag tcctgttaat taataacttt
    24661 tgttggatgt gagttatcaa caaaagcaga tttcagaatg gtttcgggcc agataactga
    24721 cgcattgtgt gaacgatttt ttcgctgttc tcccccgttg cttcttgtac ctggtacaac
    24781 atattttttt tcgttccctg tataaccaat tcgctcaaca taacttgtat tattaaattc
    24841 tttttctttt cgcttaccgt tactaaaatt tcctgtatag taacaactaa gcgcattctc
    24901 caggctcctc cctcgtttat aacaatccac gataatcttt tcgaaaacag acaagttttc
    24961 acacgaatca aggagttttt ctgcggacgt ggaataaaaa gaaaaattac tgctggtaat
    25021 ctgcatcaat ccgacagaat aacgtctttt tctgttttca atttctctga taactgttag
    25081 agcttcctcc ttacttttgg gcatatgact gataacactt ttgccatagc taccgcgttc
    25141 cctttcagga ataatttcag ccacagcata aggattgaag ccactctcga cccgtgcaat
    25201 atcatttatc gtatccggat gaactgacgc cgcacacagc aacaaggctg ggaataaaga
    25261 cgccatagct aacgctccat atatccatca atacgcatac tctgcattaa taagtaaata
    25321 atttgcttcc tcttactaag caaagatgtt ttcgttttct cttcgataaa tgcattcaac
    25381 ctgggatcga gttttatcgt gacataaacc cctttttccg atgacttatc tgatttctcc
    25441 ttcatttaat caatccacta taagtgtgtt ttggatttgt tttagatgcg aacatgattc
    25501 gcaaaataac aatgctcaac aatacgatca aataacattg acaaaatggt catattatga
    25561 ttcgtgtgat catgcaaaaa aaagcgccca tatgggcgct ctagtatgat tgaaacatta
    25621 gttaatcgaa tgtagggact ggagcatcct tctgagactg aataaacaat gcttgtttaa
    25681 cattcttaca gttctgagaa tcagagccag acttctcaca ctctgcctgc ttttgtttag
    25741 cttccgtaag atgctgcccc caccaatcca cactttttac ttcttcacta caaccaacaa
    25801 cgaagaacgg caaagcaaaa agcagatatt ttttcataaa ttccccctct cactgatcct
    25861 ggaaagactt gacacgagaa gggcattagt ccgaatgagt accagtgttc tgatccctgc
    25921 caacgagaca cctcccagtt cagctcttac agaatcgaga tattttctga cagttgaagg
    25981 ctttagtcct ttgaaatcag caatttcatt cagggtatag ccataagcgt acatcagaat
    26041 aatgtctttt tgttgctcac tcaaatgttc ttcatcacta aaaatttgat gaagtccaga
    26101 aatgcagata gcatcatgct cagtcatgat aacctccctc gtaggttgtt gtgatcagca
    26161 ggtgagcaaa gtagccgctt tgttcacctg cgccaaaatc aaattaaaat cccaggcctt
    26221 tccagttcct ctcttgctgt attcttagtt ttttatgttc ttctgaatcc tttgtaaacc
    26281 cgtgttcaag agcaaacccg atattgtgac cttgctcatg ttttctggat agctgctttt
    26341 taacgtcgct ctcttccggc tgcctggttt cacgctcatg aacctgacgt tctctgttcc
    26401 gctcaagggc aatattttca atctgccagt tattgcgatg cgtttccctg tagcctgcaa
    26461 cagttccatc atcattaaac attggactac gtacaagcgt aggagccagt ttttttactt
    26521 tgataagcgt tcctggctgg agcttttcag actcgaaatg atttttcagg tctttacccc
    26581 aaatcgtaac ctgttttcca ccttttaaag tttcataagt gaggaaaggt gttcgttttt
    26641 cccctgcctt attctgatat gaagtctccc caaaatccac caccttgtaa acattccgga
    26701 gtcttttcgg attttcagcc tgtaatcgct caatctcccg tttacgttcg ggatcttttt
    26761 tccaggtagc ggtaacatca tatcccatac ctttcagttt tctatgaaaa ccgtttctga
    26821 acttcctggt ttcagattta gtaagtctta atcttcttcc tgtttcttca tcacgaagtt
    26881 taatattaac atgtacatga ggatgatctt ctttatcatc atggtatacg gcaacgaaag
    26941 catggtttgg ataagtctct ttaagaaatt ctaccgtagc tttcagagcg tcttccctgc
    27001 ttactccagc ttctggtggt ggtgaaaaga ccatgttttt cacatgatcg atgttttctc
    27061 cacgatatgt cctggagtaa tcatttcctt tagataaagt tgaagtaaac tccctattaa
    27121 agtcttctcc tttccctgtt tgctctgaac cttttccatc ataacagtaa gcctcaagtt
    27181 caccattacg ggtaatgtaa tcaataccat ttttgatgcc tgcggctgtt ttacctgagc
    27241 cagtgatttt aaaagtgact tcttttgtag gtgtccaggt atatgctttc ttgtccgcac
    27301 catgtctgac tttatgatta aaagccgatt tactggcatg tatatgtctt cccgataact
    27361 taggagattt cggatcacgc ccagtccctt tagcgcgttt aatacggtat tcctgttcta
    27421 catacacacc cataaatcac ccaaatctgt taacaacact cttggttaac gtttcaagtt
    27481 ttgaatcaat ttgcttacac agttcaatca cttcaataac gtcttttctg aaatctggat
    27541 cgttaaccgt acagaatctt tcacctttta tgataaaatg aatgttgcga ccaacacgat
    27601 ctacagcagt tgatagtttt ttaagttcct taacttcatc agggtaaaga cagacttcac
    27661 tctttaacaa agactgacgc acacgaatag caatttctct gctcatgctc atgccgtgaa
    27721 gagcagcatt acgtttaaca agttctattt catgagggta aagacgcact gataccagct
    27781 ttgctggttc attattaact gggatgttaa caggcttggt ttcaatatta agcacgttat
    27841 gcttagcaaa agcattgata ctggtaaatc cacttaaaat agcgttatgt tctgcaaatt
    27901 ttaactcatc atcacttagc ctgaacgcta tcatcttcat aagtcacccc aacagagcct
    27961 gcggcaggca caggcaaaaa catcgttttt ggacaatctg attcaaatgt taacatttga
    28021 catcggattg tctatcctgc attgattgta ccatatgtgg cactgtgcct ggctataaaa
    28081 tcgtcgctgc atatgtccag atttcgttgc tgaaagattc tttcgtcttc gaccatcagg
    28141 caaacgtata gctgaaagat ttgtgaaaga aaaaacaggc ctggcagggc tgggaattgt
    28201 tacggctgaa gccgtaacgc tttttaagtc ctcgctgcgc tgcggggctt ccggcgcaaa
    28261 gcgcctccca tgcccttaca gggcataaaa acaagggcaa ttaagcccct gcgcgatgat
    28321 tgctgacttc tttttccaga gccttattaa caaaggcatt caacgataaa tcttcttcca
    28381 tcgccatttc tgctacccga cgatgcagtt ctggatcaag cctgacgtta aacacgcctt
    28441 taaacggggt atcaggttcc tttccatcct ccacacaaga ctgtaaatac agatcaacag
    28501 atgttttaaa ctcctgttcc agttcagcta atgtagaagc ctcataagtt accaggtccc
    28561 gaataaacgc cagttttcca tacaggacat tattttcaaa atcgggttca accgtgccta
    28621 aatatccttt atattttaaa tgtttcataa taatccagcc tctttcagat tctgtttaat
    28681 tgctttcagc gtaccacctt tgatataact ttccggatgt ggacgatgca ttaatatggt
    28741 gtgattgatt tcagcattga aaaaccgcac tcttgacccc tgcatttcct ttttgacata
    28801 tcccaaagag gaaaacaaaa cgaccagttc atcccattca aacgtttttt tactgtttaa
    28861 aaactttgcc agtagcttat ctgttttccc cataaatcca tcccctatag atatgcaact
    28921 aattatagtt acacatggca tttttgtcaa aaaatacaaa ggggcagaaa gccccttttt
    28981 ttatttaaaa catttcatac ccttgcaagt agtttaacca ccaccggaat tgatcccatt
    29041 cgctaaattt cattgccgga attctggttt cccagccttt gacaagggaa tcagcaagct
    29101 gtacgatctg ccgcgttgac atttaaaaac ctcattcagt tttgattgtc gataacgtga
    29161 tcaggttggt ggaacaacct gatcacacct tgaatagtca gtcattaccc tccttctttt
    29221 ctatagttac tgtgtatccg taaagagtca aaatgcgata agcatccaac gcctcacgct
    29281 cacttttaaa tgttctgact tcgtgtaatc ctttaccttt atcaagggat acacaaaact
    29341 cataatcttc ttctacttct atctttttaa ttaatttatc aagttcggct aatgttcgtt
    29401 ttggctcaaa aagaaatgtg cattgtacaa tggcgtttaa atcttcccga tcaagctcta
    29461 acagagcatc attataagca gctttaaaac agcgattatt acgcagatca gatgcaaact
    29521 gcattgcggc ttgttttaac gttttcatga atttcccttg cattgatgtt aatagacaat
    29581 ttcagaatca atattgaaat ttccagaaaa tgcagggatg cccctgcatt cataatgctt
    29641 aacaaagaca ctcaataaaa tctttaaaac tcaagcattc atcgccatct tttaatgaat
    29701 caaaatattt ttcatattcg tccctgtaga aaaggactaa atcaacatca ctctgcttaa
    29761 gttcgccaaa ctgaggaaca attccggctt tatagtcacg aattttattg ttatcagtaa
    29821 atatgacaac atactgttgt ccagtatcct tatgcataag gataatagtg caaagcgtta
    29881 ctcgttcgaa tgtgaaaaca tgattagtgg ttgaagtaaa aacagatgag catttcattt
    29941 taaatctcct gccctgctgg gctattgtga gaggggccat tcccctttcg atgaaacaat
    30001 aatacgacct ttaagtcgta tttgcaagtt ttctcccgtt tttttactcg ataagtgatt
    30061 gttatcacgt tttttaatcc gcgtaagcgg atggaagggg cgaacgaaaa taccagggca
    30121 gcacaggagc cacatcgccc cgaagcgcgg cgcagccgct gacgaacgta ccagggtgca
    30181 caggccatcc cttcaccctg caaagcccaa ctgaccagca ggccatgatt atgctgattt
    30241 gcgccgcaga atgccgcagt tagcgcgagc gcaagcgagt ttaccccgcc ctggtgcatt
    30301 gcagcatggc ggggcgctgt actgcgcgtg tgagcgtcag cgagctacga gcgcaactcc
    30361 aggtactaat ataaaaccat cacaggtcat tttattttga ctcccttttg cagagcacgt
    30421 attcaggatg ttctcctgca agtaacaaaa gaaactcaaa ctcaaccagt gacagacttt
    30481 gactgttttg agtattaact tcctcttttt tctgccaggt tcttagccca acaccataaa
    30541 tatgactgca ttccttttgt gttaatcctg cttttaaacg taattttttt acattttcag
    30601 gtgtattatc gggtttcatt taacataaac tccttaatta aattcttaac tggcgtctca
    30661 tcgtcttccc ttaatccggc acacgtaaaa tcaatatgtt gtaattcatt ctcattcagt
    30721 acagcatcat acaatgtaat tttcgtagtt ttgttcccga ttttaacatt tgttctggct
    30781 tttatccttt ttgtaagcct gtaattgtca ccttcttttt caagaaaata agtcacatca
    30841 cctttatttt ttctcataac tgtttcaaat accctgtaat tttaacaagt tgatctatac
    30901 tcaagccccg aatctgtaaa tcataactgt cgccctgttc ggtgacttta ccgttcaatc
    30961 cgttttcagc tttaaagcct gtcaatgccc cataccagct ttcaagctgg acaagttgcg
    31021 ataacatacc tttcaggatc agaatttcgt cctttttttc cacctggtgt ttctcatact
    31081 caatcgcctg taacacctcc tgcgaagcat ttaccagaat ttttcgctcg atatcggctg
    31141 caatatcaac gggattccgt tttctggata acgtgcaaac atgatgcgga ctacgccagg
    31201 aacgactatc cacactcccc accacagaaa agcggtcttt ttccatccga atatgcacag
    31261 agtaattttt aaactgcggt gacgtcagtt ttatacgaga cgaacaatca tcaagtttgt
    31321 tcacgcgcca gccgttcccc aggatacggc agacaacttc aaagacaggg tgatattgtt
    31381 caaaaaaaga gttcatctgt ttcccttttt gctacgtggc tgctgttccc gtctttttgg
    31441 gttcgatgcc ccaaaagacg ggctggctat aatatcgggg gcttctcgcc cccgttctgg
    31501 ttacgctctg gtttccacgc gttgccaccc tgtcgccctg cgctgtccgg ttgtaccgta
    31561 ttttgcgcgg atttcttcta tcgtgtgttc ttcacgattc cagtaacttt tatgttcgtc
    31621 tggacgataa aaccattgtt ttttctttgc cgcccattta cacccgattt cttttaaagt
    31681 ttccttatgt gtaatggttt ctccactaat ccagacccag ttacctatca cttcaaatac
    31741 cagaccagat aaaccagaaa gaacgtttaa tactttttcc aggtcatcac cgtaattata
    31801 tcgtgcatgt tcatcagtgc tctggaactg atttatttta tcaatatttg ccatcaatac
    31861 atcaaaagct gcatttaccg ctttcatcag ttcagcccct aacgggttac gatctggatg
    31921 atattttaaa gcggcttttc tgtatgctgc tttgatatct ttttcagtta attcgccgga
    31981 taatccaaaa acgtttaatg cttcctggat attcattttt ttaatctcct gccctgctgg
    32041 gctattgtga gaggggccat tcccctttcg atgaaacaat aatacgacct ttaagtcgta
    32101 tttgcaagtt ttctcccgtt tttttactcg ataagtgatt gttatcacgt tttttaatcc
    32161 gcgtaagcgg atggaagggg cgaacgaaaa taccagggca gcacaggagc cacatcgccc
    32221 cgaagcgcgg cgcagccgct gacgaacgta ccagggtgca caggccatcc cttcaccctg
    32281 caaagcccaa ctgaccagca ggccatgatt atgctgattt gcgccgcaga atgccgcagt
    32341 tagcgcgagc gcaagcgagt ttaccccgcc ctggtgcatt gcagcatggc ggggcgctgt
    32401 actgcgcgtg tgagcgttag cgaaccacgg cagcctggcg gctgcaaatt gaaaaccgcc
    32461 ccggttcgtc ctgttgttgc cgcacgcccc gcagaatatg tatgctctga ggacctgttt
    32521 tattaaggac tcatcccctt atgttgaccc gcaaaagtat cgantacagn ttctgctctc
    32581 tgttggtgct gagaagctct cacaacgaga antgggactg gatgaaaatg ctcaaaccca
    32641 tggacccacc acctgcgatg gtgacgacct ccatcctgaa gcgccgtggc gatacagcag
    32701 cccttaccct gctacaggat accggggttt aatccatcct catgttgtcc ggttttactc
    32761 cccgccctct gaaacgtctg ttcacagcca accagtgctg gacgtccttc ctggatgcgg
    32821 gcggtctgcg cgatatcgag gttgaagccg tcaccaaaat gctggcctgc ggcacacgga
    32881 tactgggtgt aaaagagttc gggtgtgata acccggactg tcagcacgta aagtacctga
    32941 ccaactcatg cggcagccgt gcctgcccgt cctgcggaaa gaaggccaca gacctgtgga
    33001 cagcaacaca gctgaatcgt cttcctgact gcgactgggt acatctggtc ttcaccctgc
    33061 cggacacgct gtggccggtg ttcgaaagca accgctggct gctgaatgac gtgtgccgtc
    33121 tggcggtgga gaatctgctg tatgccgccc gaaaacgggg gctggaaccc ggtatcttct
    33181 gcgccatcca cacgtatggc cgtcgtctca actggcatcc gcatgtacat gtgtctgtaa
    33241 cctgtggagg tctgaataag catggtcagt ggaaaaagct gagcttcctg aaagacgcga
    33301 tgcgttcacg gtggatgtgg aatatgcggc agctgcttct gaaagcgtgg tcagagggga
    33361 tggcaatgcc ggagtcgttg tcacatatca cgacggaatc acagtggaga agcctggtgc
    33421 tgaaatccgg cggaaaatac tggcatgtgt acatgtcgaa gaagacagcc ggagggcgga
    33481 atacggcacg ctacctgggt cgttatctga agaagccacc aatagcggct tcccgactgg
    33541 ctcattacaa cggaggggca agcctgagct tccgttacct ggaccacaaa acgggagaaa
    33601 cggcgacgga aacgctgaca cagcgtgagc tggtcgcgag gctgaaacag cacatcccgg
    33661 agaagttttt taagatggtg aggtacttcg ggttccttgc caaccgtgtg tgtggagaga
    33721 agctgccgca ggtgtaccgt gcactgggga tggataaacc ggaaccagtg gcgaaagtgt
    33781 gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc gttcgaatgc gtgctgtgtg
    33841 gctgccggat ggtataccgc cgggccattg cggggctgaa tgtatcaggg ctgaagaaaa
    33901 acgcgcggga tatcagtcta ctgaggtata tgccggcctg acacaggcta agtgtgcccg
    33961 ggaatcggtg aaagtataaa aaacagcctg ttacggtata aaaatcgacc atacagagag
    34021 ggaaaccacc ggagtggtgg gaaaagcaga aaggctcacg tggcataagg cgtggacaat
    34081 ggtatcgact ggtttttcaa tttcctatac gtgaactacg ggcgcaatgc aggagaacta
    34141 acagaacct
//
