LOCUS       NZ_KX452394             2989 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Escherichia coli strain FA27 plasmid pFA27, complete sequence.
ACCESSION   NZ_KX452394
VERSION     NZ_KX452394.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227083
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Cunha,M.P.V., Cerdeira,L., Lincopan,N. and Knobl,T.
  TITLE     Coexistente of CTX-M-55, CTX-M-2, CMY-2, fosA3 and qnrB19 in
            different virulent clones of ExPEC isolated from chickens in Brazil
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Cunha,M.P.V., Cerdeira,L., Lincopan,N. and Knobl,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JUN-2016) Pathology, USP, Av. Prof. Orlando Marques de
            Paiva 87, Sao Paulo, SP 05508270, Brazil
COMMENT     ##Assembly-Data-START##
            Assembly Method :: clcbio genomics workbench v. 7.5
            Assembly Name                     :: pFA27_1
            Coverage                          :: 1000x
            Sequencing Technology             :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:35:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX452394.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FA27"
                     /host="Gallus gallus"
                     /db_xref="taxon:562"
                     /plasmid="pFA27"
                     /geo_loc_name="Brazil"
     gene            730..879
                     /locus_tag="HTJ68_RS00005"
     CDS             730..879
                     /locus_tag="HTJ68_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(1888..2532)
                     /locus_tag="HTJ68_RS00010"
                     /old_locus_tag="pFA27_1_02"
     CDS             complement(1888..2532)
                     /locus_tag="HTJ68_RS00010"
                     /old_locus_tag="pFA27_1_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2626..>2781)
                     /locus_tag="HTJ68_RS00015"
                     /old_locus_tag="pFA27_1_03"
                     /pseudo=""
     CDS             complement(2626..>2781)
                     /locus_tag="HTJ68_RS00015"
                     /old_locus_tag="pFA27_1_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1892..2532)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          522..695
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(52..543)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 agtttagcct taacaggcgc ataacttcaa gactaactcc tctaaatcag ttaccagtgg
       61 ctgctgccag tggcgataag tcgtgtcttt ccgggttgga ctcaagatga tagttaccgg
      121 ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccatc caggagcgaa
      181 ctgccttcgc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga
      241 atgacaccgg taaaccgaaa ggcaggaatg cgggggagca cgagggagcc accaggggga
      301 aacgcctggt atctttaagc cgcatcgggt ttcgccacca ctgatttgag cgtcagattt
      361 catgatgttt gtcagggggg cggagcctat ggaaaaacgg cttcgccgcg gccttatctc
      421 ttccctgtta agctccttcc tggtatctcc caggaaattt ccgccccgcc tgtaagccgg
      481 caccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaata
      541 tatcctgtac tacatatcct gctgacgcgc tggtgccgcc ttttttctcc tgctacatga
      601 agcacttcac tgatttccac atccgtgcca acatagtcag ccagtataca ctccgctagc
      661 gctacgtgac tggttcaggg ctgcgccccg aaacccgcta acaacctctc tgctactgga
      721 tatcacccca tgccatctga atatgatgaa caacggggtt cgactgatac gcagcttccc
      781 ggcgaatatg acctttttga tttaatggcg aaggttactg aagagatcct gcatgcccct
      841 gtggattctg ggccgcctgt cggccgggaa gcgttatagc tttttcgctt gcccgcgtac
      901 gtggtaatac gccggtatta ccttttattt ctttattttc atgtaattac agtgggtttt
      961 taatacctgg gtattactcg tgaaaacgaa acattacttt tttttctttt aattcatgca
     1021 gttgcgatga aaatcgcggc tgcgttaggt gtatgaccat tttgaggttg aaaaataatc
     1081 aaccatggcg ggtgatttac gcaggttaag gggagatcgt gacaaaaaaa agcgttcctc
     1141 actgtatcgt tcatgcctgc atgaacgggt tattcttcct gactacagaa ttacatcgct
     1201 ccttcagttc atacatcact tttacaacac ttttacaaca ctccttcggc ctgtacatca
     1261 cttttacaac acttttgcat cacttttaca tcatttatac atcactttta catcattcct
     1321 tcagctcata cgtcactttc atcctctgtg ccatttgtac atcaccccct tcctgaacgt
     1381 gctgacagcg ggttttccgg ctgcgccggc aacgcttcac ggccacgcaa taaattgcgt
     1441 ccccgttcac cgttttcgga tgggaagaac gccggaaaca ggacttcccc tggctgaatg
     1501 gtgtgaattt ccgcgctatg cacttgcgcg catactcatg catgccgtaa aaacagagcc
     1561 tgcgcgtttc tggcgggttt tcgggtggtt tgttgcctgt tttaccggtt tcccgtcaga
     1621 aacgccctca gagcctctca ggcggtgcac gcaagggatg ttatgtttaa tcggtctgac
     1681 cgtcaggtac aaaaaaaagc ccttaggggg cttttttttt gctatgagtt agccatattt
     1741 tcgtgcgaat ttttcatcgg aatcgcatag ataaataata aactcaataa acgcgatgat
     1801 cgcgggtatg aatgtccagc aaaaaattag atacaggaac cccttaaaag taaatcgtca
     1861 aacgcatctc ccggtgtaaa cgctgaacta accaatcaca gcgatgccaa gccgctccat
     1921 gagcaacgat gcctggtagc tgtctaactt aacgccttgt aaatcaacac cccgaatatc
     1981 taagtcacct aactccgaat tggtcagatc gcaatgtgtg aagtttgctg ctcgccagtc
     2041 gaaagtcgaa aactcgccgc cggagagatc tgaaccactg aacgtcgcac ccagtacctg
     2101 agtccccatc cagcggtttt cccacagctc acacttttcc aacacgactt tcgaaaaatt
     2161 ggcgtagctt agattagtat tagtgatata tgcgctgcaa aaccaggtgc gcgtggtgat
     2221 catattcata aagcttgcac cgcggaaatc tgcgccttgt gcgcggcagt ggcgaatttc
     2281 aatgcccaag gcactgacgt tgcggaaatc tgccattgat aaatcacagc ttttgaaaat
     2341 ggcatctttc agcattgcgc gactaaaatt gcaccctttc tgactttcgc gatcatagaa
     2401 ctggcagccg ataaattcag tgccgctcag gtcggcacct gaaaaatcgc agttaaaaaa
     2461 tgtactattt tcaactttct caccggtgaa gcgatttctg tcaatttttt cgccaactaa
     2521 tgccagagtc atatctatgc ctgtgttttt atacagtaat gacgccatgg taaaccttat
     2581 gacgttatgc gtcaaacccg ccagtatgac acctgcaaat gcgtattaga tctggtgttt
     2641 cttcagcaag gcacgtagct gatgataagt cagacccagt aattcagcgg cttttttctg
     2701 gttaaattta gcctgctgta agctggtttg tagaaagtct ttctcttgct gctgctggaa
     2761 ttcacgcaga tccagcgtta taacgatgtg tatcctatgt gctcggattg gatagatttc
     2821 cattacggtg aaggatcttc ttgagatcct ttctgtggcg tcgtaatctc ttgctctgta
     2881 aacgaaaaaa ccgccctgca aggcggtttt tcgaaggttc tcagagcagc aactctttga
     2941 accgtggtaa ctggcttacg aggagcgcag ctgttctttc agtttagcc
//
