LOCUS       NZ_KX257264             2702 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Escherichia coli strain Ec17183 plasmid pEc17183, complete sequence.
ACCESSION   NZ_KX257264
VERSION     NZ_KX257264.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227136
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Falcone-Dias,M.F.
  TITLE     Dynamic flux of ColE1-type replication plasmids from the open
            environment to the nosocomial niche
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Falcone-Dias,M.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Biological sciences, School of
            Pharmaceutical Sciences, UNESP- Univ Estadual Paulista,
            Rod.Araraquara-Jau km 1, Araraquara, SP 14801 - 902, Brasil
COMMENT     ##Assembly-Data-START##
            Sequencing Technology             :: ABIPrism 3100 BioAnalyzer
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:05:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX257264.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec17183"
                     /isolation_source="clinical"
                     /db_xref="taxon:562"
                     /plasmid="pEc17183"
                     /geo_loc_name="Argentina"
                     /note="qnrB19-harboring small ColE1-type plasmid"
     gene            1016..1252
                     /locus_tag="HTH90_RS00005"
     CDS             1016..1252
                     /locus_tag="HTH90_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            complement(1757..2401)
                     /locus_tag="HTH90_RS00010"
     CDS             complement(1757..2401)
                     /locus_tag="HTH90_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(2495..>2650)
                     /locus_tag="HTH90_RS00015"
                     /pseudo=""
     CDS             complement(2495..>2650)
                     /locus_tag="HTH90_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(1761..2401)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          678..851
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(208..699)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gatcttcttg agatcctttc tgtggcgtcg taatctcttg ctctgtaaac gaaaaaaccg
       61 ccctgcaagg cggtttttcg aaggttctca gagcagcaac tctttgaacc gtggtaactg
      121 gcttacgagg agcgcagtaa ccaaatctgt tctttcagtt tagccttaac aggcgcataa
      181 cttcaagact aactcctcta aatcagttac cagtggctgc tgccagtggc gataagtcgt
      241 gtctttccgg gttggactca agatgatagt taccggataa ggcgcagcag tcggactgaa
      301 cggggggttc gtgcatacag tccatccagg agcgaactgc cttcgcggaa ctgagtgtca
      361 ggcgtggaat gagataaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
      421 ggaatgcggg ggagcacgag ggagccacca gggggaaacg cctggtatct ttaagccgca
      481 tcgggtttcg ccaccactga tttgagcgtc agatttcatg atgtttgtca ggggggcgga
      541 gcctatggaa aaacggcttc gccgcggcct tatctcttcc ctgttaagct ccttcctggt
      601 atctcccagg aaatttccgc cccgcctgta agccggcacc gctcgccgca gccgaacgac
      661 cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc ctgtactaca tatcctgctg
      721 acgcgctggt gccgcctttt ttctcctgct acatgaagca cttcactgat ttccacatcc
      781 gtgccaacat agtcagccag tatacactcc gctagcgcta cgtgactggt tcagggctgc
      841 gccccgaaac cggctaaaag ctaatgacgc gcctgcggct tgtccaacgc ctcacggcac
      901 cggattaaaa tccgctcccg ttcaccgttt ctgactgcct ttcaccgtcg ctggcgcttc
      961 atcgtttcta ctgtgtctgg cagactgtgc tgatttattc tgactggaga acgttatggc
     1021 cagactgaca aacttgacgc ctgcagagaa aaaatttctt gatgatgctg ttgccgcagc
     1081 tgaaagagcg tcaggtaaaa agctaaatca gcctaaccgg cacatcgtcc tgaacagagc
     1141 gcgtgcgcaa attgagttgc agcgttacgc cgaccgccag cgtgcattac gtgaggatga
     1201 gcggcagcag tccgattttg cctggtcacg tccgcgcgcg ccgcgccgct gattttggct
     1261 tttggcggtt tagtggtgct gacgctgcga aatcacctgc cattcacctg cgtgcatagc
     1321 catgcagtcg cgcagaaatc atcaggggcg tttttccggg atactggtga ggaaaggtgc
     1381 tggaaacggc cctgatgcag caggattgcg ctgagggcgc ctgcgggggt gcgggcaagg
     1441 gatgttatgt ttaatcggtc tgaccgtcag gtacaaaaaa aagcccttag ggggcttttt
     1501 ttttgctatg agttagccat attttcgtgc gaatttttca tcggaatcgc atagataaat
     1561 aataaactca ataaacgcga tgatcgcggg ttagatattc atcaaattcc tgtccagaat
     1621 gcagcattag atggcatgtc gttgatgcgg taggtcagga tattcgtcca ctggagaaaa
     1681 cgtttcacaa tgcttgccag gagtcgtact ggttaaaagt aaatcgtcaa acgcatctcc
     1741 cggtgtaaac gctgaactaa ccaatcacag cgatgccaag ccgctccatg agcaacgatg
     1801 cctggtagct gtctaactta acgccttgta aatcaacacc ccgaatatct aagtcaccta
     1861 actccgaatt ggtcagatcg caatgtgtga agtttgctgc tcgccagtcg aaagtcgaaa
     1921 actcgccgcc ggagagatct gaaccactga acgtcgcacc cagtacctga gtccccatcc
     1981 agcggttttc ccacagctca cacttttcca acacgacttt cgaaaaattg gcgtagctta
     2041 gattagtatt agtgatatat gcgctgcaaa accaggtgcg cgtggtgatc atattcataa
     2101 agcttgcacc gcggaaatct gcgccttgtg cgcggcagtg gcgaatttca atgcccaagg
     2161 cactgacgtt gcggaaatct gccattgata aatcacagct tttgaaaatg gcatctttca
     2221 gcattgcgcg actaaaattg caccctttct gactttcgcg atcatagaac tggcagccga
     2281 taaattcagt gccgctcagg tcggcacctg aaaaatcgca gttaaaaaat gtactatttt
     2341 caactttctc accggtgaag cgatttctgt caattttttc gccaactaat gccagagtca
     2401 tatctatgcc tgtgttttta tacagtaatg acgccatggt aaaccttatg acgttatgcg
     2461 tcaaacccgc cagtatgaca cctgcaaatg cgtattagat ctggtgtttc ttcagcaagg
     2521 cacgtagctg atgataagtc agacccagta attcagcggc ttttttctgg ttaaatttag
     2581 cctgctgtaa gctggtttgt agaaagtctt tctcttgctg ctgctggaat tcacgcagat
     2641 ccagcgttat aacgatgtgt atcctatgtg ctcggattgg atagatttcc attacggtga
     2701 ag
//
