LOCUS       NZ_KX129783            34640 bp    DNA     circular CON 05-NOV-2023
DEFINITION  Escherichia coli strain OW3E1 plasmid pOW3E1, complete sequence.
ACCESSION   NZ_KX129783
VERSION     NZ_KX129783.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227158
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34640)
  AUTHORS   Zurfluh,K., Klumpp,J., Nuesch-Inderbinen,M. and Stephan,R.
  TITLE     Full-Length Nucleotide Sequences of mcr-1-Harboring Plasmids
            Isolated from Extended-Spectrum-beta-Lactamase-Producing Escherichia
            coli Isolates of Different Origins
  JOURNAL   Antimicrob Agents Chemother 60 (9), 5589-5591 (2016)
   PUBMED   27324774
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34640)
  AUTHORS   Zurfluh,K. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2016) Institute for Food Safety and Hygiene
            Zuerich, University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: SMRTanalysis v. version 2.3
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/05/2023 12:58:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 41
            CDSs (total)                      :: 41
            Genes (coding)                    :: 40
            CDSs (with protein)               :: 40
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX129783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OW3E1"
                     /db_xref="taxon:562"
                     /plasmid="pOW3E1"
     gene            7..873
                     /locus_tag="HTI86_RS00005"
     CDS             7..873
                     /locus_tag="HTI86_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            886..1290
                     /locus_tag="HTI86_RS00010"
     CDS             886..1290
                     /locus_tag="HTI86_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            1425..1952
                     /locus_tag="HTI86_RS00015"
     CDS             1425..1952
                     /locus_tag="HTI86_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(2343..3089)
                     /locus_tag="HTI86_RS00020"
     CDS             complement(2343..3089)
                     /locus_tag="HTI86_RS00020"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_077248209.1"
                     /translation="MGGGVGFVMPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVA
                     DWFYLGMGHWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKW
                     LVPVTKFRVRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRN
                     TIPDKCFPAAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSH
                     TVVSMMLAWAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(3137..4762)
                     /locus_tag="HTI86_RS00025"
     CDS             complement(3137..4762)
                     /locus_tag="HTI86_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            complement(4856..5560)
                     /locus_tag="HTI86_RS00030"
     CDS             complement(4856..5560)
                     /locus_tag="HTI86_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_225236207.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLQIINTLKCIFSMGLRND
                     CLIKFLKYFCIA"
                     /name="DUF2806 domain-containing protein"
     gene            complement(5601..6829)
                     /locus_tag="HTI86_RS00035"
     CDS             complement(join(5601..6514,6514..6829))
                     /locus_tag="HTI86_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_085959875.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASN
                     GLSDNRCLEI"
                     /name="IS3 family transposase"
     gene            complement(7057..7326)
                     /locus_tag="HTI86_RS00040"
     CDS             complement(7057..7326)
                     /locus_tag="HTI86_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            complement(7401..8048)
                     /locus_tag="HTI86_RS00045"
     CDS             complement(7401..8048)
                     /locus_tag="HTI86_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            complement(8711..8806)
                     /gene="dqlB"
                     /locus_tag="HTI86_RS00205"
     CDS             complement(8711..8806)
                     /gene="dqlB"
                     /locus_tag="HTI86_RS00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            complement(9589..10293)
                     /locus_tag="HTI86_RS00050"
     CDS             complement(9589..10293)
                     /locus_tag="HTI86_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(10380..10844)
                     /locus_tag="HTI86_RS00055"
     CDS             complement(10380..10844)
                     /locus_tag="HTI86_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(10862..11068)
                     /gene="hha"
                     /locus_tag="HTI86_RS00060"
     CDS             complement(10862..11068)
                     /gene="hha"
                     /locus_tag="HTI86_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            complement(11065..13218)
                     /locus_tag="HTI86_RS00065"
     CDS             complement(11065..13218)
                     /locus_tag="HTI86_RS00065"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            complement(13223..13795)
                     /locus_tag="HTI86_RS00070"
     CDS             complement(13223..13795)
                     /locus_tag="HTI86_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            complement(13799..>13993)
                     /locus_tag="HTI86_RS00075"
                     /pseudo=""
     CDS             complement(13799..>13993)
                     /locus_tag="HTI86_RS00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            complement(14106..14411)
                     /locus_tag="HTI86_RS00080"
     CDS             complement(14106..14411)
                     /locus_tag="HTI86_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            complement(14395..14817)
                     /locus_tag="HTI86_RS00085"
     CDS             complement(14395..14817)
                     /locus_tag="HTI86_RS00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            complement(14826..16688)
                     /gene="taxB"
                     /locus_tag="HTI86_RS00090"
     CDS             complement(14826..16688)
                     /gene="taxB"
                     /locus_tag="HTI86_RS00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            complement(16675..17703)
                     /gene="virB11"
                     /locus_tag="HTI86_RS00095"
     CDS             complement(16675..17703)
                     /gene="virB11"
                     /locus_tag="HTI86_RS00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            complement(17705..18823)
                     /gene="virB10"
                     /locus_tag="HTI86_RS00100"
     CDS             complement(17705..18823)
                     /gene="virB10"
                     /locus_tag="HTI86_RS00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            complement(18816..19724)
                     /gene="virB9"
                     /locus_tag="HTI86_RS00105"
     CDS             complement(18816..19724)
                     /gene="virB9"
                     /locus_tag="HTI86_RS00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            complement(19721..20407)
                     /locus_tag="HTI86_RS00110"
     CDS             complement(19721..20407)
                     /locus_tag="HTI86_RS00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            complement(20746..20895)
                     /locus_tag="HTI86_RS00115"
     CDS             complement(20746..20895)
                     /locus_tag="HTI86_RS00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227648764.1"
                     /translation="MSLNWILVFVDKHITSGWSRKRKLITQLVILITGITLFKVLGGIS
                     QSWL"
                     /name="hypothetical protein"
     gene            complement(20922..22016)
                     /locus_tag="HTI86_RS00120"
     CDS             complement(20922..22016)
                     /locus_tag="HTI86_RS00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            complement(22028..22744)
                     /locus_tag="HTI86_RS00125"
     CDS             complement(22028..22744)
                     /locus_tag="HTI86_RS00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_172688279.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARILSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            complement(22756..25503)
                     /locus_tag="HTI86_RS00130"
     CDS             complement(22756..25503)
                     /locus_tag="HTI86_RS00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            complement(25522..25848)
                     /locus_tag="HTI86_RS00135"
     CDS             complement(25522..25848)
                     /locus_tag="HTI86_RS00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            complement(25862..26482)
                     /locus_tag="HTI86_RS00140"
     CDS             complement(25862..26482)
                     /locus_tag="HTI86_RS00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            complement(26838..27053)
                     /locus_tag="HTI86_RS00145"
     CDS             complement(26838..27053)
                     /locus_tag="HTI86_RS00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            complement(27050..27343)
                     /locus_tag="HTI86_RS00150"
     CDS             complement(27050..27343)
                     /locus_tag="HTI86_RS00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(27420..28649)
                     /gene="taxC"
                     /locus_tag="HTI86_RS00155"
     CDS             complement(27420..28649)
                     /gene="taxC"
                     /locus_tag="HTI86_RS00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            complement(28653..29156)
                     /gene="taxA"
                     /locus_tag="HTI86_RS00160"
     CDS             complement(28653..29156)
                     /gene="taxA"
                     /locus_tag="HTI86_RS00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            complement(29517..29864)
                     /locus_tag="HTI86_RS00165"
     CDS             complement(29517..29864)
                     /locus_tag="HTI86_RS00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            complement(29861..30109)
                     /locus_tag="HTI86_RS00170"
     CDS             complement(29861..30109)
                     /locus_tag="HTI86_RS00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            complement(30414..30764)
                     /locus_tag="HTI86_RS00175"
     CDS             complement(30414..30764)
                     /locus_tag="HTI86_RS00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            complement(30855..31073)
                     /locus_tag="HTI86_RS00180"
     CDS             complement(30855..31073)
                     /locus_tag="HTI86_RS00180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005058042.1"
                     /translation="MHKDTGQQYVVIFTDNNKIRDYKAGIVPQFGELKQSDVDLVLFYR
                     DEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            complement(31608..31835)
                     /locus_tag="HTI86_RS00185"
     CDS             complement(31608..31835)
                     /locus_tag="HTI86_RS00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            complement(31822..32073)
                     /locus_tag="HTI86_RS00190"
     CDS             complement(31822..32073)
                     /locus_tag="HTI86_RS00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            complement(32070..32612)
                     /locus_tag="HTI86_RS00195"
     CDS             complement(32070..32612)
                     /locus_tag="HTI86_RS00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            complement(32717..33232)
                     /locus_tag="HTI86_RS00200"
     CDS             complement(32717..33232)
                     /locus_tag="HTI86_RS00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     ARG             complement(3141..4762)
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2..873
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(27424..28649)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(14830..16688)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(18820..19724)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19725..20407)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          complement(20950..22016)
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.6999999999999997e-172"
ORIGIN
        1 atgagaatga cgacaaataa gacttccctt tctcgcttaa caaaagtgag acatcgaaat
       61 gaattgaatt caacgctgtc aacattgcct atggctgcta aaaaagtctt atttttagcc
      121 atgtgccaga taaactctaa aaatgaattc gatgatgatc acatatttta tgtgacagtt
      181 gccgattaca ttaaatgggt tcaggttaag cctgatgcgg cttatcttgc tttgagggat
      241 ggctctaata tattagatac gacgcttctt aagctgaaac atgatgaaat attagaactg
      301 agtagtgatt tgggatttaa gttcactaaa agtaatgtac ctgattcaat gaatttgagt
      361 ctgactgttt tttctactta ttacaggaat gaaggtagag tggggatcaa gtttacgaaa
      421 gaagcgaaaa gatttttatg taaacttatt ggtgaagaaa aacgttatac aacgcaagtt
      481 ttattatctg ttgttcgact gacaagtgtt aatgctgcat cactatatca actcattcgt
      541 aaaatatata gtaataattc tcgcgcgaat agttttgaaa tgacaataga tgaacttaaa
      601 gatgaattga atctttatac aataggggcg gggggggtga aggattataa gtatccagat
      661 tatcctgctt ttaaaagaga tgttcttaat aaatcagtaa aagaaatcat gaaacatacg
      721 gaagtaaaga acttaagttt cgtagtttca gaaaaaattg ggagaaaagt ttataaatta
      781 aagttctctt atacaattgg ttatgagggc gataccaggg aagactcaga gtttactaat
      841 atgtttgata aaatgtaccc acctgaaaat tgaggtcata acaatatgaa ggataatatc
      901 gtagattttc aaagaatcag ggacagcatg aatgatcatt caaccgatgt tgcaaatgat
      961 gccgtttatt ctgacaaaaa gaaaatatcc aggttctttt atctgctgac acctgtcaga
     1021 taccttctgg ctgcttccct ttatctggtt gttgttattc cattggggat tcttgcggga
     1081 tttcgtttct tgttgcagtt tttaggcggt atcgcagtga tgggtttaac tatcgcctgg
     1141 tacatgggtg cagatgtcag tagttacgtt gttcttgctg catggatcgc cttaactgtt
     1201 tgtggttgcg cagagcagat ttttgaatgg tggatcgaaa gccgttttgc gttccgtctt
     1261 tttggtatcg ggagaacgcc ggaagaatga gcgccgatct tacagacagt aaagaacaat
     1321 gcaggataga caatccgatg tcaaatgtta acatttgaat cagattgtcc aaaaacgatg
     1381 tttttgcctg tgcctgccgc aggctctttt aaaaggaata tgaaatgata tttaaccgta
     1441 ataaaaaacg agatgaacaa cataattctg ctgttgtttt gccatcagct gaaaatgtat
     1501 tctctgattt tattaagaac aatccagctt tgacaaccaa tgcttttaca gccattgggg
     1561 aggttgattg ttttactaaa gtacccttac tctcaaataa tgaatcagag tttttaggca
     1621 ttctttcacg taatattata cctgattatt ccgtttttcc caaaatacgg ctcattgaat
     1681 ttgttcgtcc tcatggtagc gaggaagctg taaaagattt aatccgtgaa gtgcagaata
     1741 taacctgtga ttttgtcctg aaatcatatg atgaaacagt cctggctgtt atccagtttg
     1801 gtgaaaccgc aaccgtcaga cagcaaaaga aaaaactgat tatccagcgc gtgtgtgcta
     1861 tgacaggaat ttcttttttc gaatttaaaa acactgtcga tatgatgggg agcgaagatt
     1921 tttgtcagtt cctgcgggat gatgaagagt aattaaaaat aagcccacat atttgcgggc
     1981 ttaacaggct ctttattagt acttgctgca tttgtgatta ctggcaacac agttttcact
     2041 gggcagtttt tgcggtcgct tttaattatg gttagtggaa ctcagacagg taactcgacc
     2101 tctttacata ttaataattg atagttttac ctggcttcct tcaacattta ttttgttgag
     2161 gaatttattt aaatacgcaa tatctggagt ggaaccgcat attttcatca attcttgacc
     2221 gataggcgta agtaaagccg ttcctattgg tagtttccag gttgatgaat catcgctttg
     2281 aaactcaact gaaaaaaccg ttccgtaata accgatacga ccgtttttac ttttaaaggc
     2341 gattaaactt gttcaccctt cttaaaaaac acccacgcaa gccctgccga catcgcccac
     2401 gcaagcatca tcgacaccac cgtatggctc aaaaaatgat cgccaataat catcttatag
     2461 cagcccatcg cccagcccaa tgccatcacc acgatgatga tcgcaccacg acggcggcgc
     2521 aaacttggcg caaacgcaaa ggcaaacagc gcaaatccgc tactggcatg cgccgcagga
     2581 aagcacttat caggaatggt gttacgcata ctatcaagca tcggcaaata cggcagcgta
     2641 ccatcaaaaa tcgtcaaatg cacagggcag accacatgag tatatgcctt caatgacgcg
     2701 acaactgtcg gcactaggat caaagtcagc accagatacg caagctcacg cacgcgaaat
     2761 tttgtcactg ggacaagcca cttggcagtg atagcattgc cctttttctc atgataagca
     2821 cggcaaatgg tcgcaaccag caagtagatc agcatcgcca tcagcagctt ttttagtcca
     2881 gaatacagta gcaaatctgg caaaaaagct tgcttggcaa ccatccaatg ccccataccc
     2941 agataaaacc aatcagcgac catcagatca atctgactgt gctcaagggt cagcgtggca
     3001 acgatcgcca tgacaagagc gatactcatc tcagcaagta ggcgtttatt tgataaatac
     3061 ggcataacaa accccacccc accgcccata atacgaatgg agtgtgcggt gggtttggaa
     3121 aaaatacagg gagaaatcag cggatgaatg cggtgcggtc tttgactttg tccgcggtga
     3181 catcaaacag ctttaataat gtcggcgtga tcgcgtcatg ggtcaggacg gtatcggttg
     3241 ccattggcgt gatgccagtt tgcttatccg tccagaaaaa tgcaggcaca ctgcgctgtt
     3301 cttttggtgc aaaggcattt ggcataccat gtagatagac accgttctca cccagacttt
     3361 cgccatgatc gctgacatac agcattgaga catcataggc attgctgtgc gtctgcagcc
     3421 actggatact ttgagcgatg aaatcatcgg tggcaagcaa ggcattgtca taagcattga
     3481 tcaaggactg atgttcgcac ttggcaagct cattaccttc acacactggc gtgaatttgg
     3541 caaacttttc atcatatcgc ttaaaatacg caggcccgtg attgcccatt tggtgcagca
     3601 tgatcagcat atctttgccg ttattggcag cgacaaagtc atctaagcca acgagcatac
     3661 cgacatcgcg gcattcgtta taaggattgg tgttgcagat ggcgttgttg gtcgcggatt
     3721 tataatcggc aaattgcgct tttggcagct tatccatcac gccttttgag tccgaattat
     3781 tatcacgcca caagatactt acgcccaagc gatccagcgt atccagcaca ttttcttggt
     3841 atttggcggt atcgacatca tactcatccg cgcccagata gctgaacata cacggcacag
     3901 aatacgccgt cgatgtgccg cacgatgtga cattgctaaa attggtcacg ccatcgatct
     3961 tggcaagctg tgggaaagta tcgcgctcat agccattgaa gctgacatga tcggcgcgtg
     4021 ccgtctcacc gacgacgaac accactaggc gtggcttacg catatcaggc ttggttgctt
     4081 gtaccgcgtc tttggcgtga taaatggtat cttttggcgc actggctttt ttatactcaa
     4141 tactggcaag cttacccacc gagtagattg gcatgatcgg attgacatag ctacgcagcg
     4201 gcttatgcac gcgaaagaaa ctggcataat gactgctgaa cgccaccaca ggcagtaaaa
     4261 tcagcgcaag acttgccacg atcaagccca atcggcgcat caaacccttg ccccaagtcg
     4321 gataatccac cttaacaaaa gccacaagca aacttggtag cacacccaaa ccaatgatac
     4381 gcatgataaa cgctgcgttt aatagatcct tggtctcggc ttggtcggtc tgtagggcat
     4441 tttggagcat ggtcgtatca tagaccgtgc cataagtgtc agtaaaataa ctggtcaccg
     4501 cgcccatgat taatagcaaa atcaacacag gctttagcac atagcgatac gatgataaca
     4561 gcgtggtgat cagtagcatc gcgccaaaga gcacgacagc gatcgtcagc acaaagccga
     4621 gattgtccgc gatgggatag gtttggctga ttttatcaaa aaaggtaaga ttggcggtcg
     4681 cggtcaagaa aacggcaaca ctcgccacaa gaacaaacgg actgaccgag cgtcggtacc
     4741 acacagaagt atgctgcatc atgagaaact actcaaaaaa taaacggtgg gataattgcg
     4801 gcaattatac tgcaattttt taagaaatcc agtacaattt atctgatata aaaaattatg
     4861 cgatacagaa atatttcaga aattttatta ggcaatcatt gcgcaatccc atactgaata
     4921 tacacttgag agtatttata atttgtaaac tataaccaat gccttgttga ataacgccta
     4981 aggagtctaa aaccgaaagt ttatcgaaag ttataccttc agtatctcca ttctcatcgg
     5041 taaataaaat tggagtgagt ttgtttgcct gccaaacaaa ctttccaaaa taagtaataa
     5101 ggttcgcttc ttctttgctt atagtggata atagttttaa ggttctacga ctaaacgaac
     5161 cttttgactt tgcttcttcg gataaaattc ttccccaaag catttgcatt tgttcgtcac
     5221 taatatcctc acactccctg aagaaagcct ctatccaatc ttcgtcaata tcagaaacat
     5281 tatcactctc agatagactt tgtgctgctt gcattgttat attttcaatg ttttcctgtc
     5341 gtttcgtttc acgtttgaga aaacgctcaa ctgcccgttt ttctattccc tcaagctcta
     5401 acctagaaat tagctctgtg cgtttagctt cagcttcagc cttcgctttt ttcctgattc
     5461 ttgtaggttc ataaagcaca ccaatagcat cacttatttt ttcaataagt tttgttcctg
     5521 gttctgataa tcctgataaa cttaaatcga taaggctcat ctagtgctcc taaatacccg
     5581 tgcaacaact ggatttgccc ctatatttcc agacacctgt tatcacttaa cccattactg
     5641 gcttgctgcc gtagatattc ccgtggcgag cgataaccca gtgcactatg cggatgccat
     5701 tcgttataat gctcgaatgc ctctgcaagg ttctttgctg ccgttaaccc gtctggtttg
     5761 ggcatgacac tgatgtagtc acgctttatc gttttcacga agctctctgc tatgccgtta
     5821 ctctccggac tccgcaccgc cgtgttcttc ggttcaagcc ccaacatccg ggcaaactgc
     5881 cgtgtttcat tagcccggta gcatgaacca ttatccgtca gccactctac tggagacgcc
     5941 ggaagctcgt tgccgaaacg gcgttccacc gctcccagca tgacgtcctg tactgtttca
     6001 ctgttgaagc cgcccgtagt gaccgcccag tgcagtgcct cacggtcaca gcagtccagc
     6061 gcgaacgtga ctcgcagttt ttctccgtta tcacagcgga actcgaaccc gtcagagcac
     6121 catcgctgat tactttcttt cacagccact ctgccggtat gtgcccgttt cgatggcggt
     6181 acagcgggtt ttcgctcaag caacagcgca ttctggcgca tgatccggta aatacgtttg
     6241 gcattgatcg caggcatacc atcaagttcg gcctgtctgc gaagcagcgc ccatacccga
     6301 cgataaccat atgtgggcag ctctccgata acatggtgta tacggagaag cacatccgta
     6361 tcatctgagt gacggctgcg gcgaccatct ttccagtcat cggttcgtct gagaatgacg
     6421 tgcaactgcg cacgcgacac ccggagacaa cggctgacta agcttactcc ccatccccgg
     6481 gcaataaggg cgcgtgcgct atccactttt ttgcacgccc gtattcaacg gcttctttaa
     6541 ggagttcatt ttccatcgtt tttttgccga gcaggcgctg gagttcttta atctgcttca
     6601 tggcggcagc aagttcagag gcaggaacga cctgttctcc tgcggccaca gcagtaagac
     6661 ttccctcctg gtattgcttg cgccagagaa ataactggct ggctgccaca ccgtgttgcc
     6721 gggcaacaag ggagactgtc attcccggtt caaaactctg ctgaacaata gcgatctttt
     6781 cctgtgtagt acgccgtctg cgtttctccg gtcctaagac atcaatcatc tgctctccaa
     6841 tgactagtct aaaaactagt attaagacta tcacttaaat aagtgatatt ggttgtctgg
     6901 agattcaggg ggccagtcta acaactgtac aagggtattt tattgcaaga gtaactaaaa
     6961 atgaatcaca actacattta aaagtgatga agttagtaca ttcagataag tatctaaaag
     7021 aaaatgataa agagtttatt gggccacttg ctatgattaa tcatttctgt cagctctgaa
     7081 ccacaaatta atgagttcgt tttcgtccag atcctgataa gccagtaatg ctgctttgat
     7141 tatttttgtc ttgggacgat gcagtctttt tgattcgtta tcaatgaatt caagcaattc
     7201 attcggtatg cggaatgaag ccagttttgt tttgttctct gtggggcgtt ttcctgcgtc
     7261 ctgaacaaat tttgtggcgt tcacttcctg gttttgttct agttctggta gttccagctt
     7321 tgccatgatg taccttctaa tctgattgtt tcacactaat gtatatcatg atgatgtaca
     7381 ttagtgtatg tcactatgat ttacagttcc agttctttca gcaagagttc gatttgtgct
     7441 tttgcctggc tggcaccgct aacattactt tttaactcgt gaacccccat tccctgattc
     7501 attgctctta caaaggcccg gagaaaagaa accgtgcttt tcattggttt aagccagtaa
     7561 tcatggctgt tgagagagtc tcttaattct tgttgttcac tgaacgtatt tggctggcat
     7621 ttatacagga gcacatgtgc agccagcctg ggatttattt tttgcgcatc cctgacaatt
     7681 tttgtcacag tctgaagact gtctgcttcc acctgggcaa gcgggtcgat cgggacaatc
     7741 acaatgtctg caacttttag tgcagttctg aactccgtgc tgtcatatcc ggcagtatct
     7801 ataatgagga tttctgctac atctgccatt tttgttattt tggcactgat gtcgccatag
     7861 gcttcattta atgcgacggg cggtaatccg gcctgttctc tgcgtttatt ccattttttg
     7921 gtcgagccat tcaggtctgt ttcaacaagc cccgttctct ttccctgttg tgagaggcaa
     7981 acagttaagt tggttgccag atgtgattta cctgtaccgc ctttatcacc accgattacg
     8041 ataatcatag taccccttgt atgtcacagt gatttactca tgtgtatttc tatgtgatgt
     8101 acgcatgcgt atgtcacatg gacatactat agtgtatgtc acaatgaatt acaatgatgt
     8161 acatcatgaa gaaatacatt attgtatgtc agtgtgatgt acccgttggt tggtcacttg
     8221 aaagcaaaag tgacagcgtt actttcggta tcctcccttg ctgcgaaacc ttgtggcaat
     8281 acagcaatga gtatgtttag ttacaacatt caaaattaag cgattagatt ccgcccgtac
     8341 gggcggggcg cgccactttc tgtagtgggg aggggcaggg aggttatgtt tgctggatga
     8401 gcggaggatg gaatgagcta ttagaatgtt gtaactaaag ggtatcccaa ttaaaggcca
     8461 cttacgcggc ctttgtctgt cggttcttcg gtctggtgac ataccacgat aaacatccca
     8521 cagcaacact cataagcatt ccggataagc gatcatcgat gccaggaaac aggcaaccga
     8581 caaacatcgc aacagggaaa ggaagaagta aacagcgccc caaaatgctc ataaaatcca
     8641 taaatcctcc ttttttgtta aagggcagga agcggttcac ttcctgccct tagttacgtt
     8701 tccgttgtca tcaatcgatg aattggcgga tcagttccag aactgcaatc agcgcgtttg
     8761 caacggcgac cacagctctg agaacgatga ttaacatatt aatcatccgt ttctccagta
     8821 aaactggagg aaatcaccgt tgcttgctca tacactgcct gtcacaacgg ctctctcgca
     8881 ttgttaggtg tagagtgacc tcctcctgcc agagcaccga atacagcacc acctgtttat
     8941 ccagccagga ctttaaaatc gttcataccc gcagatacgg gaacagcagg attgcaagcc
     9001 aactacatga ctaactgact gattatacgc tacacaaaac acccgttaca ccactaaatg
     9061 tggttcctta accacattta gttgctttac agcaccatag tgaataaaat gtaaacgtta
     9121 ggtgtagagt aaccttctcc ggtcagagcc accaactcaa gactggaaaa cgcaaaaaag
     9181 cccgattacc agtcgggttt ttttgtgcct gtcgttcggc tgtagttccg ttttttctcc
     9241 ttcatttgag ggatttttct ttcatttttt ttgttgcttc caatactgcc tgtcgcgttg
     9301 gccaacagct tgacgcgtta ccggcatttt cattactcaa tatttttcag tgaattaaaa
     9361 tgcaacagcg acagaaaagg acattcagct ttttagatga atgctatagc aacggtgtgc
     9421 tacgtaaaat ctgcgaaaaa agcaggggta gggtaggtta tttttcacgt agcaacgcca
     9481 tctggcactg tacataggta tgtcacacag acatacctat gtgtatgtct gtgtgaggca
     9541 ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt caaaggcctt acatttcaaa
     9601 aactctgctt accaggcgca tttcgcccag gggatcacca taataaaatg ctgaggcctg
     9661 gcctttgcgt agtgcacgca tcacctcaat acctttgatg gtggcgtaag ccgtcttcat
     9721 ggatttaaat cccagcgtgg cgccgattat ccgtttcagt ttgccatgat cgcattcaat
     9781 cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg
     9841 tttgagcaga gcaagcgcgc gaccataggc gggcgcttta tccgtgttga tgaatcgcgg
     9901 gatctgccac ttcttcacgt tgttgaggat tttacccaga aaccggtatg cagctttgct
     9961 gttacgacgg gaggagagat aaaaatcgac agtgcggccc cggctgtcga cggcccggta
    10021 cagatacgcc cagcggccat tgaccttcac gtaggtttca tccatgtgcc acgggcaaag
    10081 atcggaaggg ttacgccagt accagcgcag ccgtttttcc atttcaggcg cataacgctg
    10141 aacccagcgg taaatcgtgg agtgatcgac attcactccg cgttcagcca gcatctcctg
    10201 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
    10261 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
    10321 ataagtttat caccaccgac tatttgcaac agtgccctgt gtgatgtata cctggcgggt
    10381 tattctgcct gttccggctt agttttctca atgaggaaat cttccatgaa atgcccctca
    10441 tcaagcagct tctgtaaagc aagcggacgc ttgccattac cagaccattc tctgatctcg
    10501 ccattttcat ccttaaagcg gtatttagcc tttcttactc cgcctttggc tttccgcctg
    10561 gtttttactg tatctgctgt tacaggagca aggagtgatt cgggatcaag acctaaagat
    10621 atgatgtatt caatggcctg ctgacgtttg gcttcaagtt cctggcgttc agccgcttca
    10681 cgttcagcat cttcacgacg ttcttcaata acaacgttga gcttttcctg catttcgagc
    10741 aactgctcaa aatcaatctc acgggcaaaa acacgcaatg aacgaatatt catcatagta
    10801 cggctgataa tgccgtattc atcttcttta gtcagttcgc tcatataatt aatcctctta
    10861 tttagtgtac gtacttccag acgccggaag gcaccttatc ataaagttta ttcatcacca
    10921 gttctgctcg gcgatgatct gcggcagaat aaaatgcctc tgattctgat aaaggcagtt
    10981 tgtaacggtt aatctcaata actttttcaa gagtatctct tgatgtacat tttcttaact
    11041 gaaaaagcca ttcctgcttt gttttcactt cttagccctt gccggaaaac ccagcttccc
    11101 tgttttctta tcctcaagga caagaactgt atcatacgtt ccaccacttt tctttttaaa
    11161 tcccttaatc aaatcggttt tccctgattt aaccagtttt tcggcctgtg cctgggtgat
    11221 tttcttacca gaaaactcac tccagatttt aaattcacat ccggtacaga aataaccttt
    11281 cggcctgata acaatatgtt taccacagga agggcaggga gagtccaggc gttgaaactg
    11341 ccctgctggc gcagcgggtt caatcttcat ctcgcccagg tcaacatcag aaatcatgcc
    11401 tgtcagttca ccgtacagat cattaataaa ctgttcaacc gtcaggtcgc cattttctat
    11461 ggcagcctgc ttttcagacc ataatgctgt catatcagga ttaaccgcta taccaggcag
    11521 ggcatcaata agcgcatatc cggtatcagt aggaataagt ttcccttttt ccagcgtgat
    11581 atagtttctt ttcttcagcg tttccagaat ggctgcgcgg gtagctggcg taccaatgcc
    11641 accatgttca tcttttttgt ctttatcctt atccttcaac aattttttaa ttgttggatc
    11701 agtgacaaaa tccgcgacac gaacaagcgc agcaagcaag gaggcttcgg tgaataacgg
    11761 cggcggtgtt gttttcttct cattaacaat aacttctttt gtcgtcagtg tttctcctgt
    11821 gcgaatttta cagagcagtt caaaagcgga atcatcatta tcttctgatt caccttcgtc
    11881 tgtggtttcc gcgccaagaa acgcctcaaa tccgctgtca gttgtttttc tggcacgggc
    11941 atagaacgac tcatcaccac actgaatggc aaccgatact tcctggtatg ctttttcagg
    12001 catgaactga acaagatagt gttgtgcgat cgccaggtaa acattgcgct cgtcggtgct
    12061 gagtgcgtta acatcaggca cactggcggt cgggattatc gcagtatgcg cagtcacctt
    12121 tgcactgtta aacgctttgc ttttacgtgc agaatcaata tccagcgact gaggaaagac
    12181 tgatttcagg gcatcgataa cctgcggcgc ttcgctgaat tgttcatctg aaagatatga
    12241 gcagtcagag cggttatacg taattgcttt atatttttcg cgtagttgtt gcgtaatatc
    12301 cagcgttttt tgtgccgtca ttttaaactt cttgttcatg tattgctgga gtctgaccag
    12361 attaaacggc aacggcgcag ccgttttttt atcatcagtt gctgctgctt caactgtagc
    12421 cggttttcct gcaagggatg cagccgttcc gtctgcccac gctttatcaa gtaatttacg
    12481 gtctgtcagc ggagcaaatt cccctggctt ccagttcgcc ctgataacat cagcaccacg
    12541 ctgaaagact ccggtcatgg tgtagtaaaa actggattta tggttctggt tagcacgggt
    12601 acgattcaca atcaggccaa gaacgggagt ctggacgcgc ccgacagaca gaacgccctg
    12661 atatcctctg gcttttgccg gaatggtgta cgcacgtgtc atagagaagc catagacggc
    12721 atcggcaact gaccgcgcca acgccttaag gtaaagtcct ttgaaatcac gattgtcttt
    12781 aagatttgcc agtgcctttt tcactgccgg aagcgtgttg tcgttaatca gaacgcgctt
    12841 tacgggtttt gtgttgcctg catattccag gacttcatca accagtagct gtccctcatc
    12901 atcaggatcg ccagcgtgaa caatttcagt cacgtctcca cgtctgataa gttcgagaat
    12961 cgttttaacc tgttttgctg cactttcaac aggctgatac ttcacgggat aaagacgtaa
    13021 aggaagcgtt tcaaccttcc aggctttgta ttcaggatta tagttttccg gcggttgtga
    13081 ttcgataata tgaccaaaac agttagtcac aatggcgtta tcactttcaa accagccatc
    13141 atggcgggta aaattgccac caagtgcctt aacaatatca tttgctactg cgggtttttc
    13201 tgcgataaaa agtctcattt tgttatcctt tacattgaat taatcttttg gcacaacgga
    13261 tagcgcgttc ttccgatact gtatgttgcc agttatcttt acttactact ctgaatctgc
    13321 gctcacagcc attaaaggca aaaattccct cacggttaag tatattagtt gcaatatccc
    13381 actctttaat tctcaaaaag gctgttacag caaaaccaga atacgaagca atttttgaaa
    13441 tgatgcattc atcagctttt attaacgcgg caaaaggaac tatcttttga tgaccacacg
    13501 aaatcagagg gagtgtgtga atatcataat aagctattga atgacaatcc atgcgggtat
    13561 catcaatagc tgccagccag aatcctgcct ggtcttttat ttttctgcta atatctgaat
    13621 ctgaaacacc agggtttaat aaatatccac cataacagat atggaagggg tgagcagatt
    13681 catcaatatc aggaagttct tttaccggat tttctttcca gtccttcaga acaccgaatt
    13741 caagagcatc cagcaaaaac tttcgttcaa atccatcaaa caatgcatat tgcatgtgtt
    13801 aatcctccag gtaacttaaa tagtcaggtt gtgccccgtt ctttttataa agttcttccc
    13861 tttcctctat atcttcatct gcttcctgaa cgctcagatc gagggttcct ttcaaaaagg
    13921 cacgagcatt ttttattaca tccacttatg cactaccagc agataagcac aataaaatag
    13981 ctataataga tttggttact ttcatttcta acctcagtga ttgtaactaa tcatcacatt
    14041 tcgttggcgc gaaatgtgat acttgcagta ccctgatacc cgttggcgcg gttatcaggt
    14101 tttaattaac ctctgacacg accaaactta ctcatgattt ttgatatagc agcaggatca
    14161 gccgttgaac actgattcag aaatgcttta cgggcatcaa aagttttact ccagcgaatg
    14221 cttccttttt tctttttcag aatattaaaa aaatcctttt cagcacttct gcattcactc
    14281 cctccacctt gcccgactgc ttttccatat aagcaaagta ccgaagcgca agggtcagat
    14341 gcattagcat ttgttgatat taatcctaac gccagcaagg ctggtaaaac cactctattt
    14401 tttccagtca tttttcacct cattttgtgc tttaatgaaa actattttta cctgttcagg
    14461 taaaaaactt ttctcgtttc gccattccac gactgctgtt gccccttcca tcttcaacag
    14521 atctctgatt cgaaaccaca gcccagcatc atgtgttgtc actacaatcc tgtccgcatg
    14581 agccagagcg taataaaacg tcggcgttct ggcctgtact ccggataagg aagtgatctc
    14641 ataaacccat cctttaccgc taacgtctga ctttatgaca ccgtgaaaat cggggacata
    14701 tggcaatgag ggattgatca cttcatttgc cttttcaaac tgcacgggtg ttggctctgg
    14761 cggtgatgaa caaccagcaa gaatggaaat caataaaaac actaacttct tcatcatgtt
    14821 ctcctttatg ccgctttatc gagatcagga acatctttta ctaccagata cccttttgcg
    14881 acgatttcat tttcaaatac tttctgtgga ggcataacca gctttccttt cttcctgggg
    14941 aaaatgtttg caagttccgg agcaaccttc acaaagtcac caaagaaata ctcattgaga
    15001 tagtagaatg ccttttcaca acgaatcggg ttttctccct tcattgccag gatttcttca
    15061 ctaaatttca gtgtaagtag ttcatttgtt gttaccagtg gacgctcaat aaggctttct
    15121 gattcagatc tcgatgttcc tccacgacta cgacttttgc ttttcgaacc gatagttact
    15181 gttcgtgtcc ccatcgcttc tgataactcc cgcgcatctt taacatcaga agcggaataa
    15241 acaattttgc agggtgcact tgccagtaat gttttactgc ctgcataact gtagatttcc
    15301 tcaagctgag agccgacctg gtaaacaatc aacaatttca gcttgtaacc agcaatgaga
    15361 ccagatttac tcttgatgta atctacagca ccaattgacg ggaattcatc cagtagaaaa
    15421 aggcagtcat gctttatttc cggatcatcc gttggcatct tttcaagatt cacctgtata
    15481 gcaagctgaa agaaaatatt tgttattttt tcagccaacc tggcattatc accagaaata
    15541 cagaaataaa ttgtcatccg ctttttccgc acctgccgca aatcaaaatc atttccatca
    15601 gtagcatttg ccacattggg gttagcaaac ggtgataatc cattaagaaa gttgcttttg
    15661 agttgtgctt ttgttttatc ctccatctca atgaatgcct taaggccagc gacaccaata
    15721 tcagcaagcg ttttgtcttt cccccttaat tctgaacgaa tatcattcat gaatgaaaga
    15781 agatgttcgg gttctgcacg ctcaaaaagc tgattaattt tagacattga gaagacaggc
    15841 tcaatatcaa gcgtttttag tccagcctta tcattcttaa tcataaacca taaagcgaca
    15901 gcataagatt taaaaacacc acccgccgtc ttattaagat agctaccagc atccgcacca
    15961 tctgtcttat aggggtaaat aatcaacgaa agattttcaa gctgattaaa accttcactc
    16021 gttcccatgc tgacataata caaagggtta aagcggtgcg ttaccgaact aaagggatca
    16081 aatttataaa cctcatgacc taaaatcacc ttcctgataa gactggtgat attaaaacac
    16141 tccctttttg gatcgagaat aataacactc caccagtact tcataagatt agtgatgact
    16201 ataccagcac ctttaccagc ccttgttccg gctcccatag cactgaatgc tgctcccaca
    16261 tagcgtatga ttttcttgtc ttttatacca acgataatcc cgtctttttc gtcaccgtca
    16321 agcgtgaaac tgttagactc acgaatatca ttaattgttg caaatcgcgc atcaccgaaa
    16381 atattttctt tcttctcgtt cagttttatc aacgccgcaa cagggatgat aagagacacg
    16441 acaaatcctg ctccaagtgc aacaaaggct aaaactctga tatccgtagt cacaacagat
    16501 aaagtaaggc tgtctgtaaa aatatccatc ctgtacgcat caatggatct gaacacagcc
    16561 ttcaatggtt tcagattctt tatctggctg aagtaaagca caaagaaaat aaatgaacct
    16621 gcaaaccatg ccgcaacaag cataacagcg aatataaaaa cagattgcga cttattaaaa
    16681 gttttcacat tcagcccctt tgtaatagat gtcgctcata tgcctgacaa caccatccct
    16741 gcctacatga acaacaatat caatactgtc cataatgatt ttacgaagaa catcaaaagg
    16801 aagattctga cattctctgt tctgataaca ttttgtcacc atctgaatga tggcatcttt
    16861 tgccgaggcc gcgtgaatgg acgtcatgct tccactgtga ccagaaccag caactttcac
    16921 aaaatcccag gcatcgccac ctttaatttc cgccagtaaa attctgtcag gtttcatcct
    16981 caaacaggat ttgattaatt tcgctgctgt tatgatagag ccggaatcac tgccggattc
    17041 cgaagggtaa aaaagatgca cataattttt atggataaaa aatttgattt cctctgcatc
    17101 ttcaatcgta atcagccgtg tattcaatgg tatgaaatcg ataagactct tcatataagt
    17161 tgttttaccg gaacccgttg ctccggcaaa tacaatggtt ttccctgctg ctacggcaag
    17221 ctccataaaa gcagcgatat ttctcgctct gaataaatca agaagctcat catcgtatgt
    17281 cctgtgtttt tttcctgact ggacataatc ataaaaaccg ttcgccacat attctttatg
    17341 tgttatctgc cgtgtcgatg gtttacgtat agtgattgaa atagtatttc tctcacatgc
    17401 aggaggaaaa acgacctgta cacgttctcc tgattccagt gttgctgaaa gtaatggctt
    17461 ggtctcgccg atttcatctc cccgaaatga tgccagcgta tgagaaaaac gtttacaaaa
    17521 atcaaaggtc acactgtcat tattatggaa tgtccagtca ccattaattt ccgtccagat
    17581 ttcaccagga cggttgacag caatttctgt tagcccttca atatcaagaa aagactggaa
    17641 tacttgattt ttataaaaat cgagcgattt attgctaatg ctgacaggaa taacgccact
    17701 catactattt tacctttaac tcatagatat cagaaaaatc aatgtcttcc ccgaccataa
    17761 tagtgattat gtcaccctgg tttttataca ttgttggagg aatatttatc gaattctcaa
    17821 gtgcgacttt agccatttca gccattgcct gtcgtgagtt ttcggtataa tccacattac
    17881 gatctttacc aggtgcatta tccgccgccg ccgccgctac atcctgaacc atactgagca
    17941 taagagaacc accaaaacgc tcccagaagt gagaatcgat ccatccatca atgcccgctt
    18001 ctcccagggg accagctgct gctgtattta ccatatcgat tcgtttatta tcaggcgtcc
    18061 gcaactgctc ccacataacg aacattctgc cctgtccatg attcagcgtt ccggtctggt
    18121 aacgaccaaa agccttagtt ccctgttcca gtaattttgt gtggtgatta gcactccata
    18181 catcttctgt aaaaacacaa ctgatacgac ctgcaacatc agaaacaaag cgtgtggtta
    18241 aagcacacgg tataagcctg ttttcctcaa tatagagatc aggatcgaga ttaatcgcag
    18301 ttactttcat tcctgattta ccaggcacat tttcttttga ttctttcctt tcttcctgtg
    18361 gctctgatgt ctgcgatgtc ctgctgctgc cactcgcgcc agtctgactc cctccctgac
    18421 cagcaacagg tattgacaaa tatttcctga acccctcctg tggcacttct ttatgaccgt
    18481 cagaaacagt attaccagaa ccagtagcgc ctggattgat aattgctttg ttctcaacag
    18541 ggtcaaaatt ctgaccaaga ccagtacgga ttttacttac ctgagaaatg ctggtatcgc
    18601 ctgttgattc tgatgctttc tgctggtcat ctgcaaaata atttttatat gttttcagcg
    18661 caaaaatacc aataactaat acagccatca gaccaaacag aaagccagaa cgcatacgat
    18721 tatttttgtt cctgatatca ccgatatcat gaacgggttt actttccagc tttgactgat
    18781 tcgcatcatc aactaaatca tctgtttttt tcatatcagt cattattcac ctcaacacgt
    18841 tctaccgcag gagaagatgt attcctctgg tcggttaata cttttccgaa agatttattc
    18901 acaacaccaa caacgctgtt tccatagcgt aaaacaaatt tgtcattaac gttgtggata
    18961 accataattt tatacttgcc ttttgtttcc acattaaaag caagtgtctg ttcctgacca
    19021 tctcttacag caaatggagc tgggaatact ttccctgagt taaaacctaa aaaggtaaat
    19081 atgccattat cataagcaaa atcaggggcg atacgacgac tgtcatattt ttcattgact
    19141 cgcatgaaat aattccagtt tttaggcgca tcggcttttt caaaactttc agaaatcagt
    19201 ttcttctgtc tgagtttttc ctgtttttct tttgccagcc ttacttcttc tgcattcttt
    19261 ttcgctattt ctgatggata gcggaattgt acaacataag caggttgcgc ttgtttttct
    19321 tctgacagaa ggattagatc cagactgtaa atccgctttg ttgttctgac aaaaagattc
    19381 gtatcccatt ccttttctgt tggctggaaa acctttttca gtttttcgcc ttcatcgcct
    19441 tcctgttcct gaacaacggg acgcggattg atgtaaacaa cattatcatc ggttgtgatt
    19501 tcccatccgg caggaaaacc agcctttgca ctgataacag cctcaccttc atcaaaaaca
    19561 actgatgtga gaaatcctgt ctttgaccgg ataacggtcg tattcgcacc gttataactc
    19621 acagtctgca tccttgcatc aaatccagaa ggtaacggag tcgctgctga atacacaagc
    19681 ccaggaagca ataaacatgt agcaataaat aatttcttca tcattccccc ctgacttccc
    19741 gatctgtctg gtaactggtt acagtaaaac cgagaggatt aagctcacga ctggaacttt
    19801 taaccgactt ttctggttca aaacgatatg tcacacgagc cgtccagtat gaaactgaaa
    19861 cctgtccggt cgtgaagttt ctcgtcgttt ttttaaagcg cacagaggcc agtttatctg
    19921 gcgcggttgc gtcagaaata atattggaga tgacctcaac cgcaacgtaa gaaccgttat
    19981 tatgataaat tttatcagga gccatatcgc tgttatacag acgaagataa tcgtccttta
    20041 ctgtattcga gctatatagc tgtacagtct cataatcata ctgaagagac ggataattat
    20101 aaccttcacg taaagtaata tacgtgttta caaaataccg cccaagtgct tcagattgca
    20161 ggatcttttc ctgtttaacc tgagtaatca gttctgtacg gcctgttttt tcgccaacag
    20221 ccacaatagc aagctctttt tcttttaatg gcacaagaac acaaattgca gccccaagac
    20281 ataaggacag taatgatgct gccactgcaa cacgccatgc atttttctta gaccgttcat
    20341 tttctgcata gagttgtctt tcgaaggatt tagctgtctc aatcttcttt tcgatttttt
    20401 cactcataac tgattacctg gctgtggtta atgggttcca gttcgccgga aacatcagaa
    20461 ggaaggttat tccttgtggt acatccggaa atctgaaaca ggaaaaacaa aagaatcaca
    20521 catcgcataa attcacctta ttaagcgttc acgaacacct ttaagagata aaggccaaga
    20581 tgaagaaaaa gaatcccacc aataagaagc aaaaagcgaa gatccatacc ctttctccat
    20641 ttccccgcaa gccctaaacc aagtcttatt cctgcgaagc aggtaaacac tcctaaaaga
    20701 aggattagga caaaataaag tatcatgaca aaataatgaa gcatgtcaca accagctctg
    20761 actgattcca cctaagacct taaataaggt aattcccgtg attaaaataa caagctgggt
    20821 tattagcttt ctcttcctgc tccatccgga ggtgatatgt ttatcaacga acaccagtat
    20881 ccagttcagg gacatcacat aaaaaaaacg tttcattatt ttcatgcggc gttcctcgct
    20941 cgtgcttttt ccagggatgc aagtcttctc gccttcatcc ccaaagcacc tgtcggagcc
    21001 agcgcctgat atcccttttc acccgcccat tcagcggctg ttcgcgcacc acgccccatc
    21061 agattccctg ctttccctga taaagaattc atgcccgttc ttccggccat ccctaagcca
    21121 aaacctaaac cagcacgacc gccaagcatc agagacttac cagtagccat accagcagca
    21181 cccagcccca tcatggctgc gccctgaaca gcaccttcaa cacctgcacc tgctaactgg
    21241 acagcaaaac ctttagctat aagaaccaat aaagccccca aaaacccagc cataaagccc
    21301 atagcacctg tagatacaag atttacattc cctgtttgtg cagcacgaat agcatgggaa
    21361 aggatcatcc cttgaaaatc cattgctatc cgaatcacta aactggcaaa tagaactgtc
    21421 agaatactgg agaataacga ttgtaaccaa ttattaaaca tgctccgaat aaaaccaaac
    21481 atgagacaaa agataaatat cggtgctgta ataatcagga gtttcatcgt tacatctgct
    21541 gttaaaaaaa caaccacact aacagccatt aaaataagcg agccagtcca tattaaaaac
    21601 tgaccaacta caccttgatc tttaacatat gttgatttat ctaaagagta tatttccgca
    21661 ccaagatttt gcgtactttt ccataagtta tctaaagttt gccagacact cacccctcca
    21721 gaaaaaccat ctttcatgcc ttgcaacgca tcagtagccg cagttaaata cccatccgca
    21781 ttagttataa acatgattat gattgcaaat tttgatagat cccatactaa atcctgcaag
    21841 ggcgtctgag ttttactggc aagtatttga tatcctttcc atagtacata taacgtaacc
    21901 gataccgttg ctaacgacat aatatcatta gcaaattgtg tttgataact ggagctgatt
    21961 ttatctaccg aatccataac ggtagaatta tatttaacga aaaacccctg agccatatat
    22021 ccacctttta atcaaaagtt aatggttcag cttctttctg ctgtttaata aaggcctctt
    22081 gttcttgtgt gaccaacatc tcatgatctg ctttattttt attattccag acatcatact
    22141 gcatttttat cgcctgcatt tttaacgact gcgcctgtaa tgcgttagcc aaatcctggg
    22201 attctttgat atcctttgaa gcctcaattc ggctacttaa actctgaatc tgagacattg
    22261 cacttgataa ctgcttattg atctcatttc cctgttcaat actggcagcc gttgacaaaa
    22321 tccgtgcttt acagagatta tcattacgtt catagcccgt attacacata tcgaaggctc
    22381 catacttgct gaacagattt ttagcctggc tgctcaatgc accttcggga ttactggtaa
    22441 aatcagaaat ataactgttc ccctgcttat aaatatttgt cagttctgat tgtagattac
    22501 ttagttcatt tgtgaatgcc gcaatattcc ttatacctgt tgccgtggct aactggtctt
    22561 tatatgcctg aatctgactc tgataatgtt gcgcagtttc ggaccattgt ttgagtcgtt
    22621 ctgtccatct tgcggcttca acagcccatt ccggactggc atcaatagca accggaattc
    22681 ctgcgctact ataaaatgaa gccgtcaata atgatatcag catcaatgtt tttacacgtt
    22741 tcataaattc acctgtcaga ttgcccgttc gagatattca ggaacccagt catcaggctc
    22801 cattccttca tgataaatac tttcaaaaat ttccagatta tctgcactgg cagacaatat
    22861 tttcagatat ccaccaagtc ccgataaatc gagtgtcgca agtgctgaaa atgatttacc
    22921 atcccccttt ttaagactgc tttttttaat caggaactgt ctcgacattg gatcaagatt
    22981 tttaacaata ttaaattctt ctggtgtcag cttaagccct tccacataat cattgtaatc
    23041 ggcatcaggg ttagccagat aaatactggt gctgcatact tcaaccacag cccgtgaaat
    23101 cttattcttc agtatttcat ccggagactg agttgccgga ataaccagcc cgttcagctt
    23161 acgaattgtt ttcagcttgt tatagacaaa gtcgctgaat gcttcatcct gtagccattt
    23221 ccagaactca tccaggaaga taacaagacg ccgaccgtcc agaagctgcg taatgcgata
    23281 cagcagatag aaagtaattg gtgcacatac catcggatca tcaagaaact cggtgccatc
    23341 aatgccaaaa ttattgacat gctgaatatt gaatgtgtct ttagcattat caaaaaccca
    23401 gccatgagcc tgaccattag cccattggga caaacgaagg ataatcccgt tctcctgctc
    23461 atcccgatca tctctctgca taagatgctc aagcattctg gtaatgccat attcccgcat
    23521 ttcgccagga ggaaaatcca tcacagcatc aacactttcg ctaataagca atctttctct
    23581 tgtcgataat ctttcaccat ttctggtaca cagaatctcc atcagttgct tgacaaaaat
    23641 acggttacgt tttgttgcct ccagagcaaa aggattccat cctgtcggct caccgctttt
    23701 tacggtgtaa tattccccac caagcattcg gatacataat tcagcccctc tgtccttatc
    23761 aagaaagaca cacgttaact tcttattttt tgcagaatca gcaaaagttt cggggttatt
    23821 gtatttctgt aatgaacagg caagatatga cagaaacatt gtcttacctg aacccgccgt
    23881 tcctataact ttagtattag caagattctt ttcacctgtt tcatctttaa acagtactga
    23941 gttatgtaaa ttcagataat acgcctgttt gcttggtgtt ttgagaatag caaccgcttc
    24001 tgtccagcag tttttatcgc gtttcccctg gtagaaatta tggaatgatg ccagttcaac
    24061 aaaatttaca ttcgataccg gagataaacg cggccttaaa tgaaaaaccg ccggaagttg
    24121 ggcaaaatat gccgctggca atgacatggt tgacaatgcc ggaataatgc ccaggtcagt
    24181 aaagtctgcc gtaattttac ttacagattc gtcaagctcc ctgatactgt ccgccattac
    24241 cattagcgtg aaatggtagt aaccaaagga tatatcaccg gacacaatat cattttttgc
    24301 cttttccagt tcctgcaatt gagtaaccgc atcatcttct gtactcttta actgtttttc
    24361 cgcacgttta atggtactca atgcttctga tttgctcatg gatgtatatg aatgcgtaat
    24421 aatatagtca gcatcagaat actggagcac atcaaaaaca ccggatgcag tctcactaca
    24481 gaaatcttta atttcaatag aacgaaagaa tttatttcca tcaatgcggc atatctgtcc
    24541 ggtatcatga ttaaaaaata catccactcc accaagaacg ttatacaccg gagaatcagt
    24601 gactctgatt ttttgcaact tccctgtcag aagataatta tagaatgaca gttgagaaga
    24661 aaataccttt ccgttctcct catacatacc aagaagttta caggtaaatt tatcaagagc
    24721 gccagagaat gtattaatca tttcattcat tctcttcaca tgaatgttaa tatcatcttt
    24781 cttttcttta atactttttt tgcgtttttc cagtttttct acacgcccat ctggtctgta
    24841 gatcagcgta aagtaaagag tgttcccctt gaatttattt ttcttcatcg aaccatagta
    24901 acaatctgat attatatcag catatttatt acctgatttt gtggtaaagg catcataaaa
    24961 actcgccctg catgaatggt tataaaatga aacgggcagt ccggaaaatg aacgaacaag
    25021 agtggcaacc tgtgagtcaa tagtttctaa catctccgca gagattgact caaagttaat
    25081 ccccatcagt tgccatgtcg ctacataatc accgttcttt gttttgacaa tatttttatc
    25141 aacatggctt gaatagggta tatatttacc agtagtgatt cgctcattca gtttcatggc
    25201 attaacaaac tcatcaattt caacagcttc atattgtccg gagaggaacg ctcgtgcgcc
    25261 aaaaaaacga ttacacactg agtttccgag cattttcagc ttaagaaagt aaaggttaaa
    25321 aatgtggtcg tcctgtttag tgatcattct gagaacaaaa accgcgacag gaatagccag
    25381 ccagatcagc ttgctgatat aaacccccgc cagaaataac gcccctgcaa ccacagtaag
    25441 agggacgaca ggaacaccca catacatagc agggcgtgtc atcgctttat aaagtgtaga
    25501 catactatcg tccccgcttt attagttaac catcattgat gcaacttcag ccgcactggc
    25561 gataacaatt gccccgatga tgataggtga gcattcacga atcgtgcttc caccaaacag
    25621 aaccttgtag ccgacccaca gacaggcaat agtcaccgtg accagtgaaa ggccactaag
    25681 ccctgtttta accttttcca gcagtgtttc cgcttttgaa aatccatccg caaaggccgg
    25741 tgatgcaact gttgccccgg cgactgccat aactacctta tttttacgct gcattacagc
    25801 agtgacagat gaactgatac gattacatac tgatttctta aatgacattt tataagtcct
    25861 gttaattaat aacttttgtt ggatgtgagt tatcaacaaa agcagatttc agaatggttt
    25921 cgggccagat aactgacgca ttgtgtgaac gattttttcg ctgttctccc ccgttgcttc
    25981 ttgtacctgg tacaacatat tttttttcgt tccctgtata accaattcgc tcaacataac
    26041 ttgtattatt aaattctttt tcttttcgct taccgttact aaaatttcct gtatagtaac
    26101 aactaagcgc attctccagg ctcctccctc gtttataaca atccacgata atcttttcga
    26161 aaacagacaa gttttcacac gaatcaagga gtttttctgc ggacgtggaa taaaaagaaa
    26221 aattactgct ggtaatctgc atcaatccga cagaataacg tctttttctg ttttcaattt
    26281 ctctgataac tgttagagct tcctccttac ttttgggcat atgactgata acacttttgc
    26341 catagctacc gcgttccctt tcaggaataa tttcagccac agcataagga ttgaagccac
    26401 tctcgacccg tgcaatatca tttatcgtat ccggatgaac tgacgccgca cacagcaaca
    26461 aggctgggaa taaagacgcc atagctaacg ctccatatat ccatcaatac gcatactctg
    26521 cattaataag taaataattt gcttcctctt actaagcaaa gatgttttcg ttttctcttc
    26581 gataaatgca ttcaacctgg gatcgagttt tatcgtgaca taaacccctt tttccgatga
    26641 cttatctgat ttctccttca tttaatcaat ccactataag tgtgttttgg atttgtttta
    26701 gatgcgaaca tgattcgcaa aataacaatg ctcaacaata cgatcaaata acattgacaa
    26761 aatggtcata ttatgattcg tgtgatcatg caaaaaaaag cgcccatatg ggcgctctag
    26821 tatgattgaa acattagtta atcgaatgta gggactggag catccttctg agactgaata
    26881 aacaatgctt gtttaacatt cttacagttc tgagaatcag agccagactt ctcacactct
    26941 gcctgctttt gtttagcttc cgtaagatgc tgcccccacc aatccacact ttttacttct
    27001 tcactacaac caacaacgaa gaacggcaaa gcaaaaagca gatatttttt cataaattcc
    27061 ccctctcact gatcctggaa agacttgaca cgagaagggc attagtccga atgagtacca
    27121 gtgttctgat ccctgccaac gagacacctc ccagttcagc tcttacagaa tcgagatatt
    27181 ttctgacagt tgaaggcttt agtcctttga aatcagcaat ttcattcagg gtatagccat
    27241 aagcgtacat cagaataatg tctttttgtt gctcactcaa atgttcttca tcactaaaaa
    27301 tttgatgaag tccagaaatg cagatagcat catgctcagt catgataacc tccctcgtag
    27361 gttgttgtga tcagcaggtg agcaaagtag ccgctttgtt cacctgcgcc aaaatcaaat
    27421 taaaatccca ggcctttcca gttcctctct tgctgtattc ttagtttttt atgttcttct
    27481 gaatcctttg taaacccgtg ttcaagagca aacccgatat tgtgaccttg ctcatgtttt
    27541 ctggatagct gctttttaac gtcgctctct tccggctgcc tggtttcacg ctcatgaacc
    27601 tgacgttctc tgttccgctc aagggcaata ttttcaatct gccagttatt gcgatgcgtt
    27661 tccctgtagc ctgcaacagt tccatcatca ttaaacattg gactacgtac aagcgtagga
    27721 gccagttttt ttactttgat aagcgttcct ggctggagct tttcagactc gaaatgattt
    27781 ttcaggtctt taccccaaat cgtaacctgt tttccacctt ttaaagtttc ataagtgagg
    27841 aaaggtgttc gtttttcccc tgccttattc tgatatgaag tctccccaaa atccaccacc
    27901 ttgtaaacat tccggagtct tttcggattt tcagcctgta atcgctcaat ctcccgttta
    27961 cgttcgggat cttttttcca ggtagcggta acatcatatc ccataccttt cagttttcta
    28021 tgaaaaccgt ttctgaactt cctggtttca gatttagtaa gtcttaatct tcttcctgtt
    28081 tcttcatcac gaagtttaat attaacatgt acatgaggat gatcttcttt atcatcatgg
    28141 tatacggcaa cgaaagcatg gtttggataa gtctctttaa gaaattctac cgtagctttc
    28201 agagcgtctt ccctgcttac tccagcttct ggtggtggtg aaaagaccat gtttttcaca
    28261 tgatcgatgt tttctccacg atatgtcctg gagtaatcat ttcctttaga taaagttgaa
    28321 gtaaactccc tattaaagtc ttctcctttc cctgtttgct ctgaaccttt tccatcataa
    28381 cagtaagcct caagttcacc attacgggta atgtaatcaa taccattttt gatgcctgcg
    28441 gctgttttac ctgagccagt gattttaaaa gtgacttctt ttgtaggtgt ccaggtatat
    28501 gctttcttgt ccgcaccatg tctgacttta tgattaaaag ccgatttact ggcatgtata
    28561 tgtcttcccg ataacttagg agatttcgga tcacgcccag tccctttagc gcgtttaata
    28621 cggtattcct gttctacata cacacccata aatcacccaa atctgttaac aacactcttg
    28681 gttaacgttt caagttttga atcaatttgc ttacacagtt caatcacttc aataacgtct
    28741 tttctgaaat ctggatcgtt aaccgtacag aatctttcac cttttatgat aaaatgaatg
    28801 ttgcgaccaa cacgatctac agcagttgat agttttttaa gttccttaac ttcatcaggg
    28861 taaagacaga cttcactctt taacaaagac tgacgcacac gaatagcaat ttctctgctc
    28921 atgctcatgc cgtgaagagc agcattacgt ttaacaagtt ctatttcatg agggtaaaga
    28981 cgcactgata ccagctttgc tggttcatta ttaactggga tgttaacagg cttggtttca
    29041 atattaagca cgttatgctt agcaaaagca ttgatactgg taaatccact taaaatagcg
    29101 ttatgttctg caaattttaa ctcatcatca cttagcctga acgctatcat cttcataagt
    29161 caccccaaca gagcctgcgg caggcacagg caaaaacatc gtttttggac aatctgattc
    29221 aaatgttaac atttgacatc ggattgtcta tcctgcattg attgtaccat atgtggcact
    29281 gtgcctggct ataaaatcgt cgctgcatat gtccagattt cgttgctgaa agattctttc
    29341 gtcttcgacc atcaggcaaa cgtatagctg aaagatttgt gaaagaaaaa acaggcctgg
    29401 cagggctggg aattgttacg gctgaagccg taacgctttt taagtcctcg ctgcgctgcg
    29461 gggcttccgg cgcaaagcgc ctcccatgcc cttacagggc ataaaaacaa gggcaattaa
    29521 gcccctgcgc gatgattgct gacttctttt tccagagcct tattaacaaa ggcattcaac
    29581 gataaatctt cttccatcgc catttctgct acccgacgat gcagttctgg atcaagcctg
    29641 acgttaaaca cgcctttaaa cggggtatca ggttcctttc catcctccac acaagactgt
    29701 aaatacagat caacagatgt tttaaactcc tgttccagtt cagctaatgt agaagcctca
    29761 taagttacca ggtcccgaat aaacgccagt tttccataca ggacattatt ttcaaaatcg
    29821 ggttcaaccg tgcctaaata tcctttatat tttaaatgtt tcataataat ccagcctctt
    29881 tcagattctg tttaattgct ttcagcgtac cacctttgat ataactttcc ggatgtggac
    29941 gatgcattaa tatggtgtga ttgatttcag cattgaaaaa ccgcactctt gacccctgca
    30001 tttccttttt gacatatccc aaagaggaaa acaaaacgac cagttcatcc cattcaaacg
    30061 tttttttact gtttaaaaac tttgccagta gcttatctgt tttccccata aatccatccc
    30121 ctatagatat gcaactaatt atagttacac atggcatttt tgtcaaaaaa tacaaagggg
    30181 cagaaagccc ctttttttat ttaaaacatt tcataccctt gcaagtagtt taaccaccac
    30241 cggaattgat cccattcgct aaatttcatt gccggaattc tggtttccca gcctttgaca
    30301 agggaatcag caagctgtac gatctgccgc gttgacattt aaaaacctca ttcagttttg
    30361 attgtcgata acgtgatcag gttggtggaa caacctgatc acaccttgaa tagtcagtca
    30421 ttaccctcct tcttttctat agttactgtg tatccgtaaa gagtcaaaat gcgataagca
    30481 tccaacgcct cacgctcact tttaaatgtt ctgacttcgt gtaatccttt acctttatca
    30541 agggatacac aaaactcata atcttcttct acttctatct ttttaattaa tttatcaagt
    30601 tcggctaatg ttcgttttgg ctcaaaaaga aatgtgcatt gtacaatggc gtttaaatct
    30661 tcccgatcaa gctctaacag agcatcatta taagcagctt taaaacagcg attattacgc
    30721 agatcagatg caaactgcat tgcggcttgt tttaacgttt tcatgaattt cccttgcatt
    30781 gatgttaata gacaatttca gaatcaatat tgaaatttcc agaaaatgca gggatgcccc
    30841 tgcattcata atgcttaaca aagacactca ataaaatctt taaaactcaa gcattcatcg
    30901 ccatctttta atgaatcaaa atatttttca tattcgtccc tgtagaaaag gactaaatca
    30961 acatcactct gcttaagttc gccaaactga ggaacaattc cggctttata gtcacgaatt
    31021 ttattgttat cagtaaatat gacaacatac tgttgtccag tatccttatg cataaggata
    31081 atagtgcaaa gcgttactcg ttcgaatgtg aaaacatgat tagtggttga agtaaaaaca
    31141 gatgagcatt tcattttaaa tctcctgccc tgctgggcta ttgtgagagg ggccattccc
    31201 ctttcgatga aacaataata cgacctttaa gtcgtatttg caagttttct cccgtttttt
    31261 tactcgataa gtgattgtta tcacgttttt taatccgcgt aagcggatgg aaggggcgaa
    31321 cgaaaatacc agggcagcac aggagccaca tcgccccgaa gcgcggcgca gccgctgacg
    31381 aacgtaccag ggtgcacagg ccatcccttc accctgcaaa gcccaactga ccagcaggcc
    31441 atgattatgc tgatttgcgc cgcagaatgc cgcagttagc gcgagcgcaa gcgagtttac
    31501 cccgccctgg tgcattgcag catggcgggg cgctgtactg cgcgtgtgag cgtcagcgag
    31561 ctacgagcgc aactccaggt actaatataa aaccatcaca ggtcatttta ttttgactcc
    31621 cttttgcaga gcacgtattc aggatgttct cctgcaagta acaaaagaaa ctcaaactca
    31681 accagtgaca gactttgact gttttgagta ttaacttcct cttttttctg ccaggttctt
    31741 agcccaacac cataaatatg actgcattcc ttttgtgtta atcctgcttt taaacgtaat
    31801 ttttttacat tttcaggtgt attatcgggt ttcatttaac ataaactcct taattaaatt
    31861 cttaactggc gtctcatcgt cttcccttaa tccggcacac gtaaaatcaa tatgttgtaa
    31921 ttcattctca ttcagtacag catcatacaa tgtaattttc gtagttttgt tcccgatttt
    31981 aacatttgtt ctggctttta tcctttttgt aagcctgtaa ttgtcacctt ctttttcaag
    32041 aaaataagtc acatcacctt tattttttct cataactgtt tcaaataccc tgtaatttta
    32101 acaagttgat ctatactcaa gccccgaatc tgtaaatcat aactgtcgcc ctgttcggtg
    32161 actttaccgt tcaatccgtt ttcagcttta aagcctgtca atgccccata ccagctttca
    32221 agctggacaa gttgcgataa catacctttc aggatcagaa tttcgtcctt tttttccacc
    32281 tggtgtttct catactcaat cgcctgtaac acctcctgcg aagcatttac cagaattttt
    32341 cgctcgatat cggctgcaat atcaacggga ttccgttttc tggataacgt gcaaacatga
    32401 tgcggactac gccaggaacg actatccaca ctccccacca cagaaaagcg gtctttttcc
    32461 atccgaatat gcacagagta atttttaaac tgcggtgacg tcagttttat acgagacgaa
    32521 caatcatcaa gtttgttcac gcgccagccg ttccccagga tacggcagac aacttcaaag
    32581 acagggtgat attgttcaaa aaaagagttc atctgtttcc ctttttgcta cgtggctgct
    32641 gttcccgtct ttttgggttc gatgccccaa aagacgggct ggctataata tcgggggctt
    32701 ctcgcccccg ttctggttac gctctggttt ccacgcgttg ccaccctgtc gccctgcgct
    32761 gtccggttgt accgtatttt gcgcggattt cttctatcgt gtgttcttca cgattccagt
    32821 aacttttatg ttcgtctgga cgataaaacc attgtttttt ctttgccgcc catttacacc
    32881 cgatttcttt taaagtttcc ttatgtgtaa tggtttctcc actaatccag acccagttac
    32941 ctatcacttc aaataccaga ccagataaac cagaaagaac gtttaatact ttttccaggt
    33001 catcaccgta attatatcgt gcatgttcat cagtgctctg gaactgattt attttatcaa
    33061 tatttgccat caatacatca aaagctgcat ttaccgcttt catcagttca gcccctaacg
    33121 ggttacgatc tggatgatat tttaaagcgg cttttctgta tgctgctttg atatcttttt
    33181 cagttaattc gccggataat ccaaaaacgt ttaatgcttc ctggatattc atttttttaa
    33241 tctcctgccc tgctgggcta ttgtgagagg ggccattccc ctttcgatga aacaataata
    33301 cgacctttaa gtcgtatttg caagttttct cccgtttttt tactcgataa gtgattgtta
    33361 tcacgttttt taatccgcgt aagcggatgg aaggggcgaa cgaaaatacc agggcagcac
    33421 aggagccaca tcgccccgaa gcgcggcgca gccgctgacg aacgtaccag ggtgcacagg
    33481 ccatcccttc accctgcaaa gcccaactga ccagcaggcc atgattatgc tgatttgcgc
    33541 cgcagaatgc cgcagttagc gcgagcgcaa gcgagtttac cccgccctgg tgcattgcag
    33601 catggcgggg cgctgtactg cgcgtgtgag cgttagcgaa ctacgggcgc aatgcaggag
    33661 aactaacaga acctccctaa aacgctctga atcgcatttt aagcgatttt tttcatttcg
    33721 catggttttc ctcatggttg tatcgaacga agcggcagag cgcgattcag ggcttcttag
    33781 tggaagatcc aaaaaggatc aaataacgga tcatatgatc tgaatctctc tggttttgag
    33841 aaaggagata ccgctcgccg cagccgaacg gcaggagcgt agcgactgag tgagcgagga
    33901 agcggaattt ttgcgatgtg acattaggat cagtacgcac gaatattata tttatttcaa
    33961 taagttaaag ttaactaggg acaaatttat ggtcttgaaa attgagatac ataaggcatc
    34021 aaatattttt ttgatgattt tatgttattg aaagttatga gttttaatga ttgatgatgt
    34081 ttgttttagc ttgcgggtaa taatcatcgg gggacagatt tatggtaagg aaccatcatc
    34141 gttgctggtt ttgttgagtt atccacagtt cctgaatatt cgcacgcatt aaaagcctta
    34201 tcttttcttg tttttcttaa aaacttaaaa acttagagta gctctaattt attgatttta
    34261 agcgtgtttt ttgtctaaac atgagagctt cctcggttaa acatgataac ttcctcggtt
    34321 aaacatgaga gcttcctcgg ttaaacatga gagcttcctc ggttaaacat gagagcttcc
    34381 tcggttaaac atgagagctt cctcggtgat atccacaaaa tgtgtcataa gaatactaat
    34441 tcgttgttct ttctggtgaa tctattttga atctgttgtt aatctgcctt gaatccagtg
    34501 tggattttga gcatggatta cctttgtgag ataaatcaca gatatgaaac atgagaagat
    34561 actcggttaa acatgatatt gattgtgttg taatatcatg tttctggtgt taaagtctga
    34621 ttatcaagag tgttggaaca
//
