LOCUS       NZ_KX009021             1639 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli O25b:H4-ST131 plasmid pFV9873_2, complete sequence.
ACCESSION   NZ_KX009021
VERSION     NZ_KX009021.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227189
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Plasmid flux in Escherichia coli ST131 sublineages, analyzed by
            plasmid constellation network (PLACNET), a new method for plasmid
            reconstruction from whole genome sequences
  JOURNAL   PLoS Genet 10 (12), e1004766 (2014)
   PUBMED   25522143
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Instituto de Biomedicina y Biotecnologia de
            Cantabria, Universidad de Cantabria, C/ Albert Einstein No. 22,
            PCTCAN, Santander, Cantabria 39011, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: VelvetOptimiser
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009968825.1-RS_2024_05_16
            Annotation Date              :: 05/16/2024 01:52:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1
            CDSs (total)                 :: 1
            Genes (coding)               :: 1
            CDSs (with protein)          :: 1
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX009021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:941322"
                     /plasmid="pFV9873_2"
                     /geo_loc_name="Spain"
     gene            778..1257
                     /locus_tag="HTH62_RS00005"
     CDS             778..1257
                     /locus_tag="HTH62_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000275450.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERIR
                     ELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLESKNA
                     LVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          318..398
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(1508..1639)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.737"
                     /coverage="100.0"
                     /evalue="4.27e-51"
ORIGIN
        1 tgaaacgctc tgtaagcgtt tttcgcgcct tccatgccct gacgggcatt taaaccccgt
       61 ttcgtgcgtc tgagagcttc tgagcgcgtt tgaagggcta tctgacgact tatccccgaa
      121 agaacaacag cggaatcacc gacaacatgc gagaggtggc accgctcgcc gcagccccag
      181 cgaccgaacg ttagtgagcg agtgggcgag gaagcggaag agaaccggaa accacattga
      241 gcacgtacgc actgatgcgg ggtgtcgggg cgaagccctg accagatggc aaacggaata
      301 tcgtcgcggg tcggggcgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac
      361 ggtattacat ttgcacatcc tgtcccgatt tctgaggcgt tttagcggtg aacggacggc
      421 aaaaccatga tggagctgtt gaacgttgag agcgtgaaat ctggcaaaaa tcacagctgg
      481 taaggcttgc agaggagtaa gccagtatac actccgctat cgctacgtga ctgggtcagg
      541 gcttcgcccc gacaccccaa aaaagtgttc cgctttgcgc aacactttca gcagaaaaga
      601 tatttaaggg caattttgta agttattgat tttttagcta cttgcattat gggttagctc
      661 acgcatgtgt ggtctaaggt tagctcacgc atgtgtgagc taggtcgacc tatcatggtt
      721 tacgtttgag ctatgatggc ttatatttga gccatcatta gaccgtggag atttaccatg
      781 tatatcggga ctgaagagca gcgaaaaaag cgtctaaaag aactggaaaa attaaacaaa
      841 gaatcggaat cagatccgaa aaataacggc ccattcaccc aggtttatca gaagggctgg
      901 gaaaggatcc gagaactgtc gaaggacaag cagggagttg tagcaatacc gctttactcg
      961 tttttagctg agcacataga tccctcatgc ggtgctgttg ttgccgatca gcagttttta
     1021 gcagataaat tgggtgttag ccgaagcaca attaagcgct ggctcaacta cttagaatca
     1081 aaaaacgcgt tagtcagaat tcctgttgct ggtaaggttt gtgcgtatgc actcgatcca
     1141 catgaagtct ggaagggata caacacatct aaaaactacg cggcgtttgt cactaaaaca
     1201 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagcc
     1261 agcggcaggc acaggcaaaa accccgtttt tggacaatct gattcaaatg ttaacatttg
     1321 acatcgggtt gtctatcctg cattgctctt ttgccgcctc aaaatccctt gcgtgttttt
     1381 gctccccgat ctccagaacg atcgagccgc cacggttccg gcagcgcctt gagcgcaacc
     1441 ggaaaccgtc tggggagaac cccagacccc catcgctgta acaccgacgg tctacgacca
     1501 cctaccaacc gagtggctgc ctgtaccgtg caaatgggct ttgcaggggg ctggcgcggt
     1561 tcctggcgac ctgaaagccc ctggctaggg gtatcggcgc gcctgacctc ctgaaacgct
     1621 ctgtaagcgt ttttcgcgc
//
