LOCUS       NZ_KX009020             1598 bp    DNA     circular CON 16-MAY-2024
DEFINITION  Escherichia coli O25b:H4-ST131 plasmid pE2022_5, complete sequence.
ACCESSION   NZ_KX009020
VERSION     NZ_KX009020.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227190
KEYWORDS    RefSeq.
SOURCE      Escherichia coli O25b:H4-ST131
  ORGANISM  Escherichia coli O25b:H4-ST131
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Plasmid flux in Escherichia coli ST131 sublineages, analyzed by
            plasmid constellation network (PLACNET), a new method for plasmid
            reconstruction from whole genome sequences
  JOURNAL   PLoS Genet 10 (12), e1004766 (2014)
   PUBMED   25522143
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Lanza,V.F., de Toro,M., Garcillan-Barcia,M.P., Mora,A., Blanco,J.,
            Coque,T.M. and de la Cruz,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2016) Instituto de Biomedicina y Biotecnologia de
            Cantabria, Universidad de Cantabria, C/ Albert Einstein No. 22,
            PCTCAN, Santander, Cantabria 39011, Spain
COMMENT     ##Assembly-Data-START##
            Assembly Method              :: VelvetOptimiser
            Sequencing Technology        :: Illumina
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009968815.1-RS_2024_05_16
            Annotation Date              :: 05/16/2024 01:49:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 1
            CDSs (total)                 :: 1
            Genes (coding)               :: 1
            CDSs (with protein)          :: 1
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KX009020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli O25b:H4-ST131"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:941322"
                     /plasmid="pE2022_5"
                     /geo_loc_name="Spain"
     gene            complement(906..1382)
                     /locus_tag="HTI68_RS00005"
     CDS             complement(906..1382)
                     /locus_tag="HTI68_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(221..302)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="100.0"
                     /evalue="6.23e-34"
     Typing          1462..1570
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          417..674
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="99.0"
                     /evalue="3.93e-111"
ORIGIN
        1 acttacaaat cggcgtaaaa gatctattct aggcaagggt gttgcgcgca acgcaacgcc
       61 cttttggggt gtcggggcga agccctgacc aggtcacgta gcgatagcgg agtgtatact
      121 gacttactcc tctgcaagcc tcgtcagcca tgattttgcc cgatttcgcg atctccacgt
      181 tcaacagctc gatcatgttt ttgccgtccg ttcaccgtta aaacgcctca gaaatcggga
      241 caggatgtgg gaatgtctga ccgcgcacgc gcactcagac attccccacc tggtcagggc
      301 ttcaccccga caccccgcat ccgtgcgtaa gtgctcaatg taacttccgg ttctcttccg
      361 cttcctcgtc gattcgctcg ctacgctcgg tcatcgactt gcggcgagcg ggatcacctc
      421 tcgcatgttg tcggttatcc cgttgttgtt ctttcgggga tgagtcgtca gatagccctt
      481 caaacacgct cagaagctct cagacgcacg aaacgggttt aaatgcccgc cagggcatgg
      541 aaggcgcgaa aaacgcttac agaggctctc aggaggtcag gcgcgccgat acccctaacc
      601 aggggctttc aggtcgctag gaaccgcgtc agctccctgc aaagcctatt tgcacggcac
      661 aggcagctat tcggctggtt ggtggtcgta gaccatcggt gttacagcga tgggggtctg
      721 gggttctccc cagacggttt ccggttgcgc tcaaggcgct gccggaaccg tggcggcgcg
      781 gtcgttctgg ggatcgggga gcaaaaacac gcaagggatt ttgaggcggc aaaagagcaa
      841 tgcaggatag acaatcatgg tcaaaacgtt aacacgtagc ccctgattgt ccgcctgccg
      901 ctggctcaat ttgaaaacat ggccatgatt cggcgctgaa tatcaccgtc tttgttgacc
      961 agtgttttag tgacaaacgc tgcatggttt ttcgtagtgt tgtatccctt ccagacttca
     1021 tgtggatcga gggcatacgc acaaacctta ccagcaacgg ggattctaac taatgcattt
     1081 tttgattcta agtaattgag ccaccgaata attgtgcttc tgctaactcc aagtttttca
     1141 gctaggaatt gctgatccgc aacgacagcg ccacacgtag gatcgatatg ctccgctaaa
     1201 aatgagtaca gcttcagtgc tgatatgcct tggctatcct tcagcagctc tcgaacgcgt
     1261 tcccaacctt ttggggatac ctgtgtaaac cttgaattct tctttgcttc ttcgagatcc
     1321 tcggtgtttt tctttgcctc atctgaaagt tgtttcagtc gtttttctga atcaataaac
     1381 atgatttttt ccctctgaaa atcccatgca ctaacgttgg ttcaaatcta agccatcatg
     1441 acacatctgt gcaacacctt gttgcgtgtt gtcgcatcca ggatgcgaca acgttgtgtc
     1501 accctggatg cgacaggtaa acaaaaaaca cactaaaaaa acaacaactt acaaatcggc
     1561 gtaaaagatc tattctaggc aagggtgttg cgcgcaac
//
