LOCUS       NZ_KU932034             7939 bp    DNA     circular CON 06-MAR-2024
DEFINITION  Escherichia coli plasmid pEC16II, complete sequence.
ACCESSION   NZ_KU932034
VERSION     NZ_KU932034.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227204
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Mattila,S., Ojala,V., Ruotsalainen,P., Tuononen,T., Bamford,J.K.H.
            and Jalasvuori,M.
  TITLE     Characterization of plasmids acquired from Finnish patients derived
            ESBL strains
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Mattila,S., Ojala,V., Ruotsalainen,P., Tuononen,T., Bamford,J.K.H.
            and Jalasvuori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2016) Institute of Biotechnology, DNA Sequencing
            and Genomics, University of Helsinki, Viikinkaari 5D, Helsinki
            00014, Finland
COMMENT     ##Assembly-Data-START##
            Assembly Method                   :: HGAP3 v. 29-OCT-2014
            Sequencing Technology             :: PacBio
            ##Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/06/2024 03:04:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 12
            CDSs (total)                      :: 12
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 1 of 2
            Pseudo Genes (incomplete)         :: 1 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KU932034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pEC16II"
     gene            487..726
                     /locus_tag="HTH38_RS00005"
     CDS             487..726
                     /locus_tag="HTH38_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            723..1085
                     /locus_tag="HTH38_RS00010"
     CDS             723..1085
                     /locus_tag="HTH38_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_000181070.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="DUF4440 domain-containing protein"
     gene            complement(1110..2003)
                     /locus_tag="HTH38_RS00015"
     CDS             complement(1110..2003)
                     /locus_tag="HTH38_RS00015"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001061104.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     gene            complement(2006..3598)
                     /locus_tag="HTH38_RS00020"
     CDS             complement(2006..3598)
                     /locus_tag="HTH38_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(3637..4320)
                     /locus_tag="HTH38_RS00025"
     CDS             complement(3637..4320)
                     /locus_tag="HTH38_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228355625.1"
                     /translation="MNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEEADDIF
                     NETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAYLHHYP
                     NTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVRYSIKN
                     INTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKGCGAGC
                     SCNSR"
                     /name="hypothetical protein"
     gene            complement(4883..5041)
                     /locus_tag="HTH38_RS00060"
     CDS             complement(4883..5041)
                     /locus_tag="HTH38_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            5371..5558
                     /locus_tag="HTH38_RS00065"
                     /pseudo=""
     CDS             5371..5558
                     /locus_tag="HTH38_RS00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5701..5892)
                     /locus_tag="HTH38_RS00030"
     CDS             complement(5701..5892)
                     /locus_tag="HTH38_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5957..6280
                     /locus_tag="HTH38_RS00035"
     CDS             5957..6280
                     /locus_tag="HTH38_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955993.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6489..>6920
                     /locus_tag="HTH38_RS00055"
                     /pseudo=""
     CDS             6489..>6920
                     /locus_tag="HTH38_RS00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6958..7476
                     /locus_tag="HTH38_RS00045"
     CDS             6958..7476
                     /locus_tag="HTH38_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            7483..7716
                     /locus_tag="HTH38_RS00050"
     CDS             7483..7716
                     /locus_tag="HTH38_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          5538..5625
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4970..5518)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.479"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5925..6274
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.42e-67"
     Typing          6271..7820
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.595"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaaaacgca tacgggcgcg attctggtgt gtcttttgtt gctgtgtttt gcctctattg
       61 ccagatcgcg ctcattcccc tgaacttctg ttttcccgct ccggtccccg tcttcttcgc
      121 ttccgaaagc atgaaaaaaa tccgtttcag ctgtctctgg ctggcaattc ctgcgtgatt
      181 cacatggctg catagctatg catgtggtta aaatttaccg ggtgcgatcg cggcagtttt
      241 tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgccga acgcgtttca
      301 gcggtgcgta caataaggga ttatggtaaa ttatcttgtt ccttagagta tgattcgagg
      361 tggtatcctg cacttattta aatatgcttg cttgggaaat tccaggttgc agtatggttg
      421 agttgtaatt ttcctatgat aggaaaaatg ttatattatt aatttatttg tgaggcttca
      481 taaagaatga aaatttctga tattgaaaaa aacatatata aaggactagc tgaatttatt
      541 tcacaaaaaa tcagagatga acatgatata tgccttggac agtttgaatc agaggaatta
      601 cttgatgaga ttcttaaaag agtaatccca gtcatatata atgtggctat tgatgatgct
      661 ataaaatcag taaggaatgt tggggataga ttagaggagg aattagatat gaggaaggta
      721 gtgtgactaa ttttaaatca gacttaaatc taataagaaa agaatggtat aatgcatttt
      781 attcaggtaa tactgaaatg ctagattact tggaagctga ttggtttatt tcaacaaatg
      841 gtcaaaaagt tatctataaa aaacatcagt tacgaaagat tgatatgcgt ccagcgagga
      901 ttttacctag catagaaaga tatgaatatg atgtcgtaat gcgtgagttt aaaggcattg
      961 cttgtgtttc agggaaagct gaaataaagc aaaatgatgg aaatatatta atggcattta
     1021 ttgagaattg gataaaggtt aatggtggat ggaaaatgca gtttatatcg tatgaaaaaa
     1081 tctaagggga agccccctta gattaatggt tatatatgtt caagttttat ttttttatag
     1141 cgataagttc tgctttgagc tttaagcatg ttttctgcaa actgaatatc aaaactattt
     1201 atagcattag ttattatagt ttttattttt tctttatcag ataatgaatc accatgctct
     1261 ataggttccc gcatatatgg attgtttttt aattcatcaa ataatgtttt aaatagttct
     1321 aatcgacgag gtcttataac tgtaaaagat atatgaagag agtgtttgct ctgagtcgta
     1381 acattgtgcc agtatccagc aggaagatat atggcctgac ctggggttat atcaaactca
     1441 taatctgggg aggtattagg tggtagataa tcgaagcttc tttcatcttc gagagggtat
     1501 gaataggtag ggttgtaaac tttccatttt tttattccct caatttgaac agctattaca
     1561 tcatgattgt caaaatgaag cccaaagctt ggtgttgctg taaatgcagc atatagatta
     1621 gcactacatg tacattctaa ctctttagat aaccatagtc tgctttcatc tacagattca
     1681 aagaatgact ggcatcgatc tactataagg gttgcgccat cacgtaagca tttatatagc
     1741 tgatggcgat ttatatgtgg tgttctatcg cctgtttggc tatatgtgta tcttataaat
     1801 cctttatacc ccctaatttc gggaaagttg tcatttgcca ttctaactct aggatagtgc
     1861 aatatatctt tttcaagcaa tttattaaga tcattccagt tgattaagtt tttataatta
     1921 acatccgtga atacaaacgg atatttctgg aaattatatt tcttaaattt ttctatgatt
     1981 tcctttttta taagattatt catttttaaa cctcatgttt tgtgatatct ataatctgtg
     2041 ctttaggtat attatttata agttgttcat catgagatat aacaatgata tgcttttcct
     2101 tgttttcctc ttttatataa tcactgatta tttttatacc aagtgaatca aacccttttg
     2161 tcggttcatc gaaaataatc aagtctacgt tttttccaga tgtaagagat actcctaatt
     2221 tttgtttttg tccaccagat aaattatgtc cgtaaccttt gatctcataa tctagtggtt
     2281 tgtcgaactt aaatctgtgt agcagctttt taattgtatt taaattatta atgccggaat
     2341 aatgtaatat attttcctct atagttccag gcatgagatc tagatcttga ggaacgtata
     2401 agacattaac aaaaggagaa tttgcttttt cttgttgtat accattgatt tttattgtgt
     2461 tttcttctgt tgttatattg ccagagagaa tttttgcaat agttgttttt cccgaaccat
     2521 tctcaccggt tataaaggta acaacgcctt ttgttaatat taatttatgg ttgaatgatt
     2581 tgttgtatat ttcaatattg caaatattgt cttttacatg aagatattgc ttgtctgtgt
     2641 aatttgtttt ttttatgtcg tttatttcgg cacagtataa atcaatttct gttcttgcca
     2701 tttttatttg tctaaataga taaccaatgc gatttaatgg gagaaaaact tggaataaaa
     2761 aagtggcaag cataataata ctaccacggc ttgctctacc atgaagaaac atccagtatg
     2821 ctgataggat aatcagacta ctaaaaatga ctgaaagaaa agcggagctg ataccaaaca
     2881 ggaaaaattc attatttact tttctttgtt tttctgaatg tgtatttaca gcttgaatta
     2941 ctcgtttacg acttttatcc gtggtatgga aagctttatc cataggatac atgtgcatca
     3001 tatcaaacat tcttccagat gcgaatttgg agctaagtgc aactgatttc atcattggaa
     3061 atctacgttg tgtcattttt atgacaaata ttatatagat tgccattatt attataaact
     3121 caatggctat taatgtatta acacttatac aaattgttat gattattcca atcatttgta
     3181 ttaataatgg tactacatca gaacatattg tataaaataa agatgatgca gaagaaagca
     3241 tgctttctat aaccttattt agttctcctt tatttttctt taaaaaatgc tcttggtgag
     3301 aattttgtat attatcaata acttgctctc taagctttac agataattct atatttgttt
     3361 tttgagtcaa gtattccgat atgctatatc cagcaggcat aaggaatcta attgataaga
     3421 aacatgctat aaatattaat aatgagttta tttctcctat tgaatcttta ttgtcaataa
     3481 ctgtttttat taaatatccg aatagaacga aaagaaaact acttatagtt gataataata
     3541 taataaaaaa caaattacat ttattaaatg tgatgatgta cttttcaata aaattcaatt
     3601 tataatcctt aaagcccgta tattacgggc tttatgttat ctagaattac atgagcaccc
     3661 agcgccacat cctttaccgc tgcaaccaca aacagaacca ttatcattcc aaacatcttt
     3721 tcgcattatt atttttacaa attctgcggc tggaaggtga gtgatactct ttactttttc
     3781 gatgtaagaa tcactggata tggattcttt tggaattctt atagtattaa tgttctttat
     3841 gctgtatctt acagcataat caccatacca ctttgcgaca gtttcatgac ctaggtaaaa
     3901 agcgattttt tcaatttgat ttttatattc gttaaaacga tattcttgga atgttatacc
     3961 atgctcatta acatgcctta tctcattttt tagcatgtca tttgtatttg gataatggtg
     4021 gaggtaagca ccaagatatt tttcacaaaa gctggtataa gcatctgtaa attgaataaa
     4081 cgtatgccac atttcatcta taatttgtaa ttgctcgtga acttttatat tgtagcattc
     4141 cgttaggcat gttgaagcta gccagatgaa tttctttgtt tcattaaaga tatcatcagc
     4201 ttcttctggt gttacattcc attctttacg aaattttaga agaacatcat cattgtgata
     4261 actcatgata tcttctaatg agctaacatt atgaactttt gcataagaac tgctgttcat
     4321 cttatgttgt agcataacac ccccaattat atatttgaat gtagatgaga atagttatca
     4381 tttgttatag tattgttaaa tttgtgcttg tgtcaatatg cctaattcta taaatgagat
     4441 agtaatcaag ttttttaatg gttataggat ccattagtaa tacaaggttt ttgcagccag
     4501 tttttacggg taggggctgc aaggaaaaca aaattttccc actctaagag aagagaattg
     4561 agttggttga atgagtggat gcatctaggg ccgctaattc accgagtgta aaggctgtac
     4621 gaaatgtgtt gcagtccgat aatagccccc tccattgacg ttttacacat gtgttgtagg
     4681 ttccaggagt gggcggatct caaaaagatc aaaggatctt cttgagatcc tttttttctg
     4741 cgcgtaatct gctgccagta aacgaaaaaa ccgcctgggg aggcggttta gtcgaaggtt
     4801 aagtcagttg gggactgctt aaccgggtaa ctggcttggt ggagcacaga taccaaatac
     4861 tgtccttcta gtgtagccgc agttaggcca ccacttcaag aactcttaat atctcaatcc
     4921 accttgtcca gttaccagtg gctgctgcca gtggcgcttt gtcgtgtctt accgggttgg
     4981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5041 cacagcccag cttggagcga acgacctaca ccgagccgag atacctacag cgtgagctat
     5101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt atgatgctcg tcaggggggc
     5281 ggagcctatg gaaaaacgcc aacggcgcgg ccttttgttg gcctttgcct ttttgctcac
     5341 atgttctttc cggccttatc ccctgattct gtggataacc gtattacgcc tttgagtgcg
     5401 ctgataccgc tcgccgcagc cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
     5461 aagagcgcct gatgcggcat tttctcctta cgcatctgtg cggtatttca caccgcatat
     5521 ggtgctcttt tcttataatc tgcactgatg ccgcatagtt aagccagtat acactccgct
     5581 atccgctacg tgactggttc agggcttcgc cccgaaaccc cctgacgcgc ccttacgggc
     5641 ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccggga gctgtatgtg
     5701 tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcggc
     5761 gtggtcgtga agtgattcac aaatatcggc ctgttcatct gcgtccagtt cgttgagttt
     5821 ctccagcagc gttaatgtct ggcttctgat aaaacgggcc atgttaaggg cggttttttc
     5881 ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttttatgggg taatgatacc
     5941 gatgaaacgc gagaggatgc tcaccatacg ggttactgat gatgaacatg cccggttact
     6001 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     6061 ggttgcacgc tccgggaagc tgccgacact ggcaccgccg ttactgcgtc agctggccgc
     6121 catcgggaat aacctgaatc agacagcgag gaaggtgaac agcgggcagt ggtcttccgg
     6181 tgatcgggtt caggtgttgg ctgcactgat ggccatcggg gaggagctgc gtcggctgcg
     6241 tctggccgtc agggaacagg gcgcgcggga tgattgttaa atttcatgcc aggggaaaag
     6301 gcggtggcag tggtccggtt gattacctgc tgggaaggga gcgaaaccgg gaaggggcaa
     6361 cggtgcttca gggtgatccg gaagaagttc gggaactcat cgatgccacg ccatttgcga
     6421 agaaatacac gtcaggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg
     6481 aaaaggtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaag aatcagtaca
     6541 gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt gtcatcccga
     6601 acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgggca gacaggccga
     6661 gaattgatgc ctggcagacg ctggttaatc accattacgg gctgcatgac cccaacgccc
     6721 cggagaaccg ccggatactg actctccctg ataacctgcc ggaaacgaaa caggcgcttg
     6781 cagagagcgt cacacgcggg atagacgctc tttatcatgt cggggagata aaaggccgtc
     6841 aggatgtgat tcaggcgctc acagaggcag ggcttgaagt ggtcagggtg atgcgaagca
     6901 gtatcagcat tgcagacccg aacggcggga agaatatcag gctgaaagga gcattttatg
     6961 agcaatcttt tacagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct
     7021 acagagacaa tgctgaacga cgagttcagc aagctcgaaa aatctgtaag caaggctgtg
     7081 acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc gacagaggaa
     7141 tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag gaaggacgtg
     7201 ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcctttt cctgtttacc
     7261 gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct ggaagaaatc
     7321 aggatacagg aagaaacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg
     7381 caggacggga gaaggaaatt tctggttatc ccgcagggga aatcggcaac ggtgattccc
     7441 tatcagggga aggactgggt acaactgacg gagtgacaca gagtgacgga gctggaaaga
     7501 catttgctga acgccttaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     7561 gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     7621 ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga gcagacagaa
     7681 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atacaggaac
     7741 gcgaacatga gttatccctg gagcgtgagc gccagctgga aatacaggaa cggacacagg
     7801 atggcccttc gctgggatgg taatcagaga caggctaaaa tgttgtcagc gcgtttccgg
     7861 tgcatttttt gggataatgg ttggttcggg gcaccgggaa cgcgcttcag tcagaccttg
     7921 ctgaaaacac aggaacgcg
//
